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12. DIABETES EXPERIMENTAL

Author

Chan, J. S. d., primary, Abdo, S., additional, Ghosh, A., additional, Alquier, T., additional, Chenier, I., additional, Filep, J. G., additional, Ingelfinger, J. R., additional, Zhang, S.-L., additional, Ross, E. A., additional, Willenberg, B. J., additional, Oca-Cossio, J., additional, Clapp, W. L., additional, Terada, N., additional, Abrahamson, D. R., additional, Ellison, G. W., additional, Matthews, C. E., additional, Batich, C. D., additional, Ihoriya, C., additional, Satoh, M., additional, Sasaki, T., additional, Kashihara, N., additional, Piwkowska, A., additional, Rogacka, D., additional, Angielski, S., additional, Jankowski, M., additional, Pontrelli, P., additional, Conserva, F., additional, Papale, M., additional, Accetturo, M., additional, Gigante, M., additional, Vocino, G., additional, Dipalma, A. M., additional, Grandaliano, G., additional, Di Paolo, S., additional, Gesualdo, L., additional, Franzen, S., additional, Pihl, L., additional, Khan, N., additional, Gustafsson, H., additional, Palm, F., additional, Koszegi, S., additional, Hodrea, J., additional, Lenart, L., additional, Hosszu, A., additional, Wagner, L., additional, Vannay, A., additional, Tulassay, T., additional, Szabo, A., additional, Fekete, A., additional, Aoki, R., additional, Sekine, F., additional, Kikuchi, K., additional, Miyazaki, S., additional, Yamashita, Y., additional, Itoh, Y., additional, Kolling, M., additional, Park, J.-K., additional, Haller, H., additional, Thum, T., additional, Lorenzen, J., additional, Hirayama, A., additional, Yoh, K., additional, Ueda, A., additional, Itoh, H., additional, Owada, S., additional, Kokeny, G., additional, Szabo, L., additional, Fazekas, K., additional, Rosivall, L., additional, Mozes, M. M., additional, Kim, Y., additional, Koh, E. S., additional, Lim, J. H., additional, Kim, M. Y., additional, Chang, Y. S., additional, Park, C. W., additional, Kim, H. W., additional, Shin, B. C., additional, Kim, H. L., additional, Chung, J. H., additional, Chan, J.-S., additional, Wu, T.-C., additional, Chen, J.-W., additional, Clotet, S., additional, Soler, M. J., additional, Rebull, M., additional, Pascual, J., additional, Riera, M., additional, Patinha, D., additional, Afonso, J., additional, Sousa, T., additional, Morato, M., additional, Albino-Teixeira, A., additional, Kim, H., additional, Min, H. S., additional, Kang, M.-J., additional, Kim, J. E., additional, Lee, J.-E., additional, Kang, Y. S., additional, Cha, D. R., additional, Jo, Y.-I., additional, Seo, E.-H., additional, Kim, J.-D., additional, Lee, S.-H., additional, Jorge, L., additional, Silva, K. A. S., additional, Luiz, R. S., additional, Rampaso, R. R., additional, Lima, W., additional, Cunha, T. S., additional, Schor, N., additional, Lee, H. J., additional, Park, J. Y., additional, Kim, S. K., additional, Moon, J. Y., additional, Lee, S. H., additional, Ihm, C. G., additional, Lee, T. W., additional, Jeong, K. H., additional, Moon, J.-Y., additional, Kim, S., additional, Park, J.-Y., additional, Kim, S.-Y., additional, Kim, Y.-G., additional, Jeong, K.-H., additional, Ihm, C.-G., additional, Marques, C., additional, Mega, C., additional, Goncalves, A., additional, Rodrigues-Santos, P., additional, Teixeira-Lemos, E., additional, Teixeira, F., additional, Fontes Ribeiro, C., additional, Reis, F., additional, Fernandes, R., additional, Sutariya, B. K., additional, Badgujar, L. B., additional, Kshtriya, A. A., additional, Saraf, M. N., additional, Chiu, C.-H., additional, Lee, W.-C., additional, Chau, Y.-Y., additional, Lee, L.-C., additional, Lee, C.-T., additional, Chen, J.-B., additional, Dahan, I., additional, Nakhoul, F., additional, Thawho, N., additional, Ben-Itzhaq, O., additional, Levy, A. P., additional, Cordisco, G., additional, Fiorentino, L., additional, Federici, M., additional, Wystrychowski, G., additional, Havel, P. J., additional, Graham, J. L., additional, Zukowska-Szczechowska, E., additional, Obuchowicz, E., additional, Psurek, A., additional, Grzeszczak, W., additional, Wystrychowski, A., additional, Gimeno, J., additional, Almeida, B. Z. d., additional, Seraphim, D. C. C., additional, Punaro, G., additional, Nascimento, M., additional, Mouro, M., additional, Lanzoni, V. P., additional, Lopes, G. S., additional, Higa, E. M. S., additional, Roca-Ho, H., additional, and Marquez, E., additional

Published
2014
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14. Diabetes - experimental models

Author

Blanco-Gozalo, V., primary, Blazquez-Medela, A., additional, Garcia-Sanchez, O., additional, Quiros, Y., additional, Montero, M., additional, Martinez-Salgado, C., additional, Lopez-Hernandez, F., additional, Lopez-Novoa, J., additional, Yao, L., additional, Qing, Z., additional, Hua, X., additional, Min, F., additional, Fei, M., additional, Ning, W., additional, Cantaluppi, V., additional, Figliolini, F., additional, Delena, M., additional, Beltramo, S., additional, Medica, D., additional, Tetta, C., additional, Segoloni, G., additional, Biancone, L., additional, Camussi, G., additional, Cunha, J. S., additional, Ferreira, V. M., additional, Naves, M. A., additional, Boim, M. A., additional, Zitman-Gal, T., additional, Golan, E., additional, Green, J., additional, Pasmanik-Chor, M., additional, Bernheim, J., additional, Benchetrit, S., additional, Riera, M., additional, Clotet, S., additional, Pascual, J., additional, Soler, M., additional, Nakai, K., additional, Fujii, H., additional, Kono, K., additional, Goto, S., additional, Hirata, M., additional, Shinohara, M., additional, Fukagawa, M., additional, Nishi, S., additional, Fan, Q., additional, Du, S., additional, Jiang, Y., additional, Wang, L., additional, Fang, L., additional, Radovits, T., additional, Mozes, M. M., additional, Rosivall, L., additional, Kokeny, G., additional, Aoki, R., additional, Tateoka, R., additional, Sekine, F., additional, Kikuchi, K., additional, Yamashita, Y., additional, Itoh, Y., additional, Cappuccino, L., additional, Garibotto, G., additional, D'Amato, E., additional, Villaggio, B., additional, Gianiorio, F., additional, Mij, M., additional, Viazzi, F., additional, Salvidio, G., additional, Verzola, D., additional, Piwkowska, A., additional, Rogacka, D., additional, Audzeyenka, I., additional, Kasztan, M., additional, Angielski, S., additional, Jankowski, M., additional, Gaber, E. W., additional, El-Attar, H. A., additional, Liu, J., additional, Zhang, W., additional, He, Y., additional, Macsai, E., additional, Takats, Z., additional, Derzbach, L., additional, Korner, A., additional, Vasarhelyi, B., additional, Huang, M. S., additional, Bo, H., additional, Liu, F., additional, Fu, P., additional, Tsotakos, N. E., additional, Tsilibary, E. C., additional, Drossopoulou, G. I., additional, Thawho, N., additional, Farid, N., additional, Peleg, A., additional, Levy, A., additional, Nakhoul, N., additional, Lenghel, A. R., additional, Borza, G., additional, Catoi, C., additional, Bondor, C. I., additional, Muresan, A., additional, Kacso, I. M., additional, Song, J.-S., additional, Song, J.-H., additional, Ahn, S.-H., additional, Choi, B. S., additional, Hong, Y. a., additional, Kim, M. Y., additional, Lim, J. H., additional, Yang, K.-S., additional, Chung, S., additional, Shin, S. J., additional, Kim, H. W., additional, Chang, Y. S., additional, Kim, Y. S., additional, Park, C. W., additional, Takayanagi, K., additional, Hasegawa, H., additional, Shimizu, T., additional, Ikari, A., additional, Noiri, C., additional, Iwashita, T., additional, Tayama, Y., additional, Asakura, J., additional, Anzai, N., additional, Kanozawa, K., additional, Kato, H., additional, Mitarai, T., additional, Huang, M., additional, Ashour, R. H., additional, Fouda, A. E.-M. M., additional, Saad, M. A., additional, El-Banna, F. M., additional, Moustafa, F. A., additional, Fouda, M. I., additional, Sanchez-Nino, M. D., additional, Sanz, A. B., additional, Poveda, J., additional, Saleem, M., additional, Mathieson, P., additional, Ruiz-Ortega, M., additional, Selgas, R., additional, Egido, J., additional, Ortiz, A., additional, Soler, M. J., additional, Rebull, M., additional, Marquez, E., additional, Okazaki, S., additional, Kogure, Y., additional, Sano, T., additional, Hatano, M., additional, Kreft, E., additional, Kowalski, R., additional, Szczepansk-Konkel, M., additional, Liu, X., additional, Yang, G., additional, Osman, N. A., additional, NasrAllah, M. M., additional, Kamal, M. M., additional, Ahmed, A. I., additional, Fekih-Mrissa, N., additional, Mrad, M., additional, Baffoun, A., additional, Sayeh, A., additional, Hmida, J., additional, Gritli, N., additional, Galchinskaya, V., additional, Topchii, I., additional, Semenovykh, P., additional, Yefimova, N., additional, Zheng, D., additional, Hu, D., additional, Li, X., additional, Peng, A. I., additional, Olea-Herrero, N., additional, Arenas, M., additional, Munoz-Moreno, C., additional, Moreno-Gomez-Toledano, R., additional, Gonzalez-Santander, M., additional, Arribas, I., additional, and Bosch, R., additional

Published
2013
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15. Experimental models of CKD

Author

Kanlaya, R., primary, Sintiprungrat, K., additional, Thongboonkerd, V., additional, Torremade, N., additional, Bindels, R., additional, Hoenderop, J., additional, Fernandez, E., additional, Dusso, A., additional, Valdivielso, J. M., additional, Krueger, T., additional, Boor, P., additional, Schafer, C., additional, Westenfeld, R., additional, Brandenburg, V., additional, Schlieper, G., additional, Jahnen-Dechent, W., additional, Ketteler, M., additional, Jee, W., additional, Li, X., additional, Richards, B., additional, Floege, J., additional, Goncalves, J. G., additional, Canale, D., additional, de Braganca, A. C., additional, Shimizu, M. H. M., additional, Moyses, R. M. A., additional, Andrade, L., additional, Seguro, A. C., additional, Volpini, R. A., additional, Romoli, S., additional, Migliorini, A., additional, Anders, H.-J., additional, Eskova, O., additional, Neprintseva, N., additional, Tchebotareva, N., additional, Bobkova, I., additional, Kozlovskaya, L., additional, Simic, I., additional, Tabatabaeifar, M., additional, Wlodkowski, T., additional, Denc, H., additional, Mollet, G., additional, Antignac, C., additional, Schaefer, F., additional, Ekaterina, I. A., additional, Giardino, L., additional, Rastaldi, M. P., additional, Van den Heuvel, L., additional, Levtchenko, E., additional, Okina, C., additional, Okamoto, T., additional, Kamata, M., additional, Murano, J., additional, Kobayashi, K., additional, Takeuchi, K., additional, Kamata, F., additional, Sakai, T., additional, Naito, S., additional, Aoyama, T., additional, Sano, T., additional, Takeuchi, Y., additional, Kamata, K., additional, Thomasova, D., additional, Bruns, H. A., additional, Liapis, H., additional, Iwashita, T., additional, Hasegawa, H., additional, Takayanagi, K., additional, Shimizu, T., additional, Asakura, J., additional, Okazaki, S., additional, Kogure, Y., additional, Hatano, M., additional, Hara, H., additional, Inamura, M., additional, Iwanaga, M., additional, Mitani, T., additional, Mitarai, T., additional, Savin, V. J., additional, Sharma, M., additional, Wei, C., additional, Reiser, J., additional, McCarthy, E. T., additional, Sharma, R., additional, Gauchat, J.-F., additional, Eneman, B., additional, Freson, K., additional, Van Geet, C., additional, Choi, D. E., additional, Jeong, J. Y., additional, Chang, Y. K., additional, Na, K.-R., additional, Lee, K. W., additional, Shin, Y. T., additional, Ni, H.-F., additional, Chen, J.-F., additional, Zhang, M.-H., additional, Pan, M.-M., additional, Liu, B.-C., additional, Kim, S. S., additional, Suzuki, T., additional, Iyoda, M., additional, Matsumoto, K., additional, Shindo-Hirai, Y., additional, Kuno, Y., additional, Wada, Y., additional, Yamamoto, Y., additional, Shibata, T., additional, Akizawa, T., additional, Munoz-Felix, J. M., additional, Lopez-Novoa, J. M., additional, Martinez-Salgado, C., additional, Ehling, J., additional, Babickova, J., additional, Gremse, F., additional, Kiessling, F., additional, Lammers, T., additional, Lech, M., additional, Gunthner, R., additional, Lorenz, G., additional, Ryu, M., additional, Grobmayr, R., additional, Susanti, H., additional, Kobayashi, K. S., additional, Flavell, R. A., additional, Rayego-Mateos, S., additional, Morgado, J., additional, Sanz, A. B., additional, Eguchi, S., additional, Pato, J., additional, Keri, G., additional, Egido, J., additional, Ortiz, A., additional, Ruiz-Ortega, M., additional, Leduc, M., additional, Geerts, L., additional, Grouix, B., additional, Sarra-Bournet, F., additional, Felton, A., additional, Gervais, L., additional, Abbott, S., additional, Duceppe, J.-S., additional, Zacharie, B., additional, Penney, C., additional, Laurin, P., additional, Gagnon, L., additional, Detsika, M. G., additional, Duann, P., additional, Lianos, E. A., additional, Leong, K. I., additional, Chiang, C.-K., additional, Yang, C.-C., additional, Wu, C.-T., additional, Chen, L.-P., additional, Hung, K.-Y., additional, Liu, S.-H., additional, Carvalho, F. F., additional, Teixeira, V. P., additional, Almeida, W. S., additional, Schor, N., additional, Small, D. M., additional, Bennett, N. C., additional, Coombes, J., additional, Johnson, D. W., additional, Gobe, G. C., additional, Montero, N., additional, Prada, A., additional, Riera, M., additional, Orfila, M., additional, Pascual, J., additional, Rodriguez, E., additional, Barrios, C., additional, Kokeny, G., additional, Fazekas, K., additional, Rosivall, L., additional, Mozes, M. M., additional, Hornigold, N., additional, Hughes, J., additional, Mooney, A., additional, Benardeau, A., additional, Riboulet, W., additional, Vandjour, A., additional, Jacobsen, B., additional, Apfel, C., additional, Conde-Knape, K., additional, Bienvenu, J.-F., additional, Tanaka, T., additional, Yamaguchi, J., additional, Nangaku, M., additional, Niwa, T., additional, Bolati, D., additional, Shimizu, H., additional, Yisireyili, M., additional, Nishijima, F., additional, Brocca, A., additional, Virzi, G., additional, de Cal, M., additional, Ronco, C., additional, Priante, G., additional, Musacchio, E., additional, Valvason, C., additional, Sartori, L., additional, Piccoli, A., additional, Baggio, B., additional, Perkuhn, M., additional, Weibrecht, M., additional, Zok, S., additional, Martin, I. V., additional, Schoth, F., additional, Ostendorf, T., additional, Kuhl, C., additional, Karabaeva, A., additional, Essaian, A., additional, Beresneva, O., additional, Parastaeva, M., additional, Kayukov, I., additional, Smirnov, A., additional, Audzeyenka, I., additional, Kasztan, M., additional, Piwkowska, A., additional, Rogacka, D., additional, Angielski, S., additional, Jankowski, M., additional, Bockmeyer, C. L., additional, Kokowicz, K., additional, Agustian, P. A., additional, Zell, S., additional, Wittig, J., additional, Becker, J. U., additional, Nishizono, R., additional, Venkatareddy, M. P., additional, Chowdhury, M. A., additional, Wang, S. Q., additional, Fukuda, A., additional, Wickman, L. T., additional, Yang, Y., additional, Wiggins, R. C., additional, Fazio, M. R., additional, Donato, V., additional, Lucisano, S., additional, Cernaro, V., additional, Lupica, R., additional, Trimboli, D., additional, Montalto, G., additional, Aloisi, C., additional, Mazzeo, A. T., additional, Buemi, M., additional, Gawrys, O., additional, Olszynski, K. H., additional, Kuczeriszka, M., additional, Gawarecka, K., additional, Swiezewska, E., additional, Chmielewski, M., additional, Masnyk, M., additional, Rafalowska, J., additional, Kompanowska-Jezierska, E., additional, Lee, W.-C., additional, Chau, Y.-Y., additional, Lee, L.-C., additional, Chiu, C.-H., additional, Lee, C.-T., additional, Chen, J.-B., additional, Kim, W.-K., additional, and Shin, S. J., additional

Published
2013
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23. Uncontrolled-rate freezing and storage at -80 degrees C, with only 3.5-percent DMSO in cryoprotective solution for 109 autologous peripheral blood progenitor cell transplantations.

Author

Halle, P, Tournilhac, O, Knopinska-Posluszny, W, Kanold, J, Gembara, P, Boiret, N, Rapatel, C, Berger, M, Travade, P, Angielski, S, Bonhomme, J, and Deméocq, F

Subjects
AUTOGRAFTS, COMPARATIVE studies, CRYOPRESERVATION of organs, tissues, etc., DIMETHYL sulfoxide, FREEZING, HEMATOPOIETIC stem cells, HEMATOPOIETIC stem cell transplantation, RESEARCH methodology, MEDICAL cooperation, RESEARCH, EVALUATION research
Abstract

Background: Although controlled-rate freezing and storage in liquid nitrogen are the standard procedure for peripheral blood progenitor cell (PBPC) cryopreservation, uncontrolled-rate freezing and storage at -80 degrees C have been reported.Study Design and Methods: The prospective evaluation of 109 autologous PBPC transplantations after uncontrolled-rate freezing and storage at -80 degrees C of apheresis products is reported. The cryoprotectant solution contained final concentrations of 1-percent human serum albumin, 2.5-percent hydroxyethyl starch, and 3.5-percent DMSO.Results: With in vitro assays, the median recoveries of nucleated cells (NCs), CD34+ cells, CFU-GM, and BFU-E were 60.8 percent (range, 11.2-107.1%), 79.6 percent (6.3-158.1%), 35.6 percent (0.3-149.5%), and 32.6 percent (1.7-151.1%), respectively. The median length of storage was 7 weeks (range, 1-98). The median cell dose, per kg of body weight, given to patients after the preparative regimen was 6.34 x 10(8) NCs (range, 0.02-38.3), 3.77 x 10(6) CD34+ cells (0.23-58.5), and 66.04 x 10(4) CFU-GM (1.38-405.7). The median time to reach 0.5 x 10(9) granulocytes per L, 20 x 10(9) platelets per L, and 50 x 10(9) reticulocytes per L was 11 (range, 0-37), 11 (0-129), and 17 (0-200) days, respectively. Hematopoietic reconstitution did not differ in patients undergoing myeloablative or nonmyeloablative conditioning regimens before transplantation.Conclusion: This simple and less expensive cryopreservation procedure can produce successful engraftment, comparable to that obtained with the standard storage procedure. [ABSTRACT FROM AUTHOR]

Published
2001
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30. Localization of nucleotide pyrophosphatase in the rat kidney.

Author

Hir, M., Dubach, U., and Angielski, S.

Abstract

Hydrolysis of NAD by a nucleotide pyrophosphatase of renal membrane fractions has been reported previously. The aim of the present study was to localize this enzyme in the rat kidney. Nucleotide pyrophosphatase was assayed in glomeruli, in three parts of the proximal tubule and in four parts of the distal tubule dissected form freezedried sections. Nucleotide pyrophosphatase activity, expressed in μmol·min·mg protein, ranged between 9.8 and 32.3 in the proximal tubular segments and between 1.1 and 2.7 in the distal tubular segments. It was 3.4 in the glomeruli. The enrichement of the activity during the purification of brush border vesicles was measured. A tenfold higher specific activity was found in the brush border vesicles as compared to the renal cortical homogenates. Thus, most of the renal nucleotide pyrophosphatase appears to be localized in the luminal membrane of the proximal tubule. A permeabilization of the membrane did not increase the activity of brush border vesicles. This indicates that all catalytic sites are accessible at the outer surface of the membrane. [ABSTRACT FROM AUTHOR]

Published
1986
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31. Properties of an Ecto-5’-Nucleotidase of the Renal Brush Border

Author

Le Hir M, Dubach Uc, and Angielski S

Subjects
Male, Brush border, Renal cortex, In Vitro Techniques, Kidney, 5'-nucleotidase, Nucleotidases, medicine, Animals, 5'-Nucleotidase, chemistry.chemical_classification, Microvilli, Catabolism, Hydrolysis, Vesicle, Rats, Inbred Strains, General Medicine, Adenosine, Adenosine Monophosphate, Rats, Enzyme, medicine.anatomical_structure, chemistry, Biochemistry, Nephrology, Cardiology and Cardiovascular Medicine, medicine.drug
Abstract

A decrease of glomerular filtration rate can be observed during accelerated catabolism of ATP in kidney. It has been proposed that this effect is due to the increase in the renal production of adenosine from ATP. The last reaction in the pathway concerned is the conversion of 5’-AMP to adenosine. We found that brush border membranes purified from homogenates of the rat renal cortex carry out this reaction. The enzyme involved in the hydrolysis has the characteristic properties of ecto-5’-nucleotidases: It is inhibited by ATP, ADP, and by α, β-methyleneadenosine-5’-diphosphate, and it is not stimulated by magnesium. All catalytic sites are accessible from the outside of the vesicles. The Km of the enzyme for 5’-AMP is 5.77 µM. The enrichment of the 5’-AMP-hydrolyzing activity in the brush border fraction as compared to the homogenate is 9.2 ± 1.5 times. Histochemical staining of kidney sections reveals hydrolysis of 5’-AMP only at the brush border of the proximal tubule. We conclude that the brush border of the proximal tubule of the rat kidney possesses an ecto-5’-nucleotidase which has the same properties as the ecto-5’-nucleotidases in other tissues.

Published
1985
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32. The effect of (-)hydroxycitrate on pyruvate metabolism in rat brain synaptosomes

Author

Andrzej Szutowicz, Angielski S, and Lysiak W

Subjects
Male, L-Lactate Dehydrogenase, Chemistry, Brain, Pyruvate Dehydrogenase Complex, Citrate (si)-Synthase, Rat brain, Biochemistry, Choline O-Acetyltransferase, Mitochondria, Rats, Cellular and Molecular Neuroscience, Adenosine Triphosphate, Glutamate Dehydrogenase, ATP Citrate (pro-S)-Lyase, Animals, Magnesium, Citrates, Pyruvates, Pyruvate Metabolism, Synaptosomes
Published
1977
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33. PURIFICATION AND KINETIC PROPERTIES OF ATP: CITRATE OXALOACETATE LYASE FROM RAT BRAIN

Author

Andrzej Szutowicz, Lysiak W, Angielski S, and M Stepień

Subjects
Isocitrates, Physiological significance, Cations, Divalent, Biochemistry, Cellular and Molecular Neuroscience, Adenosine Triphosphate, Non-competitive inhibition, Allosteric Regulation, Pi, Animals, Citrate synthase, Citrate lyase activity, Coenzyme A, Magnesium, Citrates, Pyruvates, Chromatography, biology, Nucleotides, Chemistry, Aconitic Acid, Brain, Oxo-Acid-Lyases, Hydrogen-Ion Concentration, Lyase, Rat brain, Rats, Adenosine Diphosphate, Kinetics, Liver, Metals, ATP Citrate (pro-S)-Lyase, biology.protein, Ketoglutaric Acids, Sugar Phosphates
Abstract

— A method for a partial purification of ATP:citrate oxaloacetate lyase from rat brain is described. The Lineweaver–Burk plots of velocity vs citrate concentration are biphasic in the presence of fixed concentrations of MgCl2. Therefore two values of Km, corresponding to low and high concentrations of citrate, can be determined. When MgCl2 is added in equimolar concentrations with citrate, a monophasic plot with one Km of 0.13 mm is obtained. The Km value for MgATP2- was independent of citrate concentration, being equal to 0.40–0.43 mm. The Km for CoA was 0.0007 mm. ADP and Pi are competitive inhibitors with respect to ATP. Ki for MgADP− is equal to 0.13 mm. dl-isocitrate and cis-aconitate are partially competitive inhibitors with respect to citrate with Ki values of 5.8 and 4.8 mm, respectively. α-Ketoglutarate and pyruvate are noncompetitive inhibitors with respect to ATP and citrate, with Ki values equal to 9 and 45 mm, respectively. The physiological significance of these effectors for the regulation of citrate lyase activity in brain is discussed.

Published
1975
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34. Metabolism of tricarboxylic acid cycle in rat kidney medulla in vitro

Author

Angielski S, Rogulski J, and Mikulski P

Subjects
Isocitrates, Membranes, Chemistry, Citric Acid Cycle, Malates, Rat kidney, Metabolism, In Vitro Techniques, Kidney, NAD, Permeability, In vitro, Mitochondria, Rats, Citric acid cycle, Biochemistry, Physiology (medical), Animals, Dicarboxylic Acids, Citrates, Oxidoreductases, NADP, Medulla
Published
1972
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36. The purine nucleotide cycle and ammonia formation from glutamine by rat kidney slices

Author

Strzelecki, T, Rogulski, J, and Angielski, S

Abstract

To test the significance of the purine nucleotide cycle in renal ammoniagenesis, studies were conducted with rat kidney cortical slices using glutamate or glutamine labelled in the alpha-amino group with 15N. Glucose production by normal kidney slices with 2 mM-glutamine was equal to that with 3 mM-glutamate. With L-[15N]glutamate as sole substrate, one-third of the total ammonia produced by kidney slices was labelled, indicating significant deamination of glutamate or other amino acids from the cellular pool. Ammonia produced from the amino group of L-[alpha-15N]glutamine was 4-fold higher than from glutamate at similar glucose production rates. Glucose and ammonia formation from glutamine by kidney slices obtained from rats with chronic metabolic acidosis was found to be 70% higher than by normal kidney slices. The contribution of the amino group of glutamine to total ammonia production was similar in both types of kidneys. No 15N was found in the amino group of adenine nucleotides after incubation of kidney slices from normal or chronically acidotic rats with labelled glutamine. Addition of Pi, a strong inhibitor of AMP deaminase, had no effect on ammonia formation from glutamine. Likewise, fructose, which may induce a decrease in endogenous Pi, had no effect on ammonia formation. The data obtained suggest that the contribution of the purine nucleotide cycle to ammonia formation from glutamine in rat renal tissue is insignificant.

Published
1983
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38. Inhibition of alanine and aspartate aminotransferases by alpha-oxoderivatives of the branched-chain amino acids

Author

Andrzej Szutowicz, Angielski S, Lysiak W, and Maria Pienkowska‐Vogel

Subjects
Azoles, Alpha (ethology), Biology, Biochemistry, Cellular and Molecular Neuroscience, chemistry.chemical_compound, Maple Syrup Urine Disease, Leucine, medicine, Valerates, Animals, Humans, Aspartate Aminotransferases, Binding site, Amino Acids, Caproates, Transaminases, Alanine, chemistry.chemical_classification, Binding Sites, Maple syrup urine disease, Brain, Alanine Transaminase, Metabolism, medicine.disease, Keto Acids, Amino acid, Rats, Kinetics, Enzyme, chemistry, Liver, Organ Specificity, Spectrophotometry, Ultraviolet, Pyridoxamine, Protein Binding
Abstract

— 1 L-Alanine: α-oxoglutarate aminotransferase was partly purified from rat brain and liver. The enzyme from the brain has about 10 times less activity than that from the liver. 2 Both enzymes have identical apparent Km values for L-alanine, L-glutamate, α-oxoglutarate and pyruvate. Moreover they are competitively inhibited by L-leucine. α-oxoisocaproate and α-oxotsovalerate. Obtained K, values are very similar and do not depend on the course of reaction. 3 α-Oxoisocaproate inhibits the activity of crystalline L-aspartate: α-oxoglutarate aminotransferase; Kj is about 4–7 mM. 4 The pyridoxamine form of L-alanine: α-oxoglutarate aminotransferase seems to be more sensitive to the inhibitory effect of the compounds investigated. 5 The effect of branched-chain amino acids and their α-oxoanalogues on the metabolism of amino groups in maple syrup urine disease is discussed.

Published
1974

39. Lipogenetic and glycolytic enzyme activities in carcinoma and nonmalignant diseases of the human breast

Author

Angielski S, J Kwiatkowski, and A Szutowicz

Subjects
Cancer Research, medicine.medical_specialty, ATP citrate lyase, Breast Neoplasms, Biology, chemistry.chemical_compound, Breast Diseases, Internal medicine, Lactate dehydrogenase, medicine, Humans, Glycolysis, Breast, skin and connective tissue diseases, chemistry.chemical_classification, NADPH dehydrogenase, Lipids, Endocrinology, Enzyme, Oncology, chemistry, Lipogenesis, Female, Breast carcinoma, Phosphofructokinase, Research Article
Abstract

Activities of some enzymes associated with carbohydrate and lipid metabolism were determined in 48 human breast carcinomas and compared with those found in 35 nonmalignant breast tumours and also in 13 normal breast tissues. In fibrocystic disease only the activity of citrate lyase was markedly higher (14-fold) than in normal tissue. The activities of the remaining enzymes did not differ significantly from those in normal tissue. Enzyme activities in breast carcinoma were 4--160 x those determined in normal tissue according to the following sequence : phosphofructokinase less than malate NADP dehydrogenase less than hexokinase less than lactate dehydrogenase less than isocitrate NADP dehydrogenase less than ATP citrate lyase. Activity of citrate lyase, very low in normal breast (0.0017 mumol/min/g of tissue) rose gradually to 0.039, 0.072 and 0.258 mumol/min/g of tissue in localized fibrocystic disease, fibroadenomas and carcinomas respectively. These data support the idea that citrate lyase may play an important role in lipogenesis in hyperplastic human breast tissues.

Published
1979

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