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Chickpea and lentils are one of the most important legumes not only as sources of food and nutrients but also for enrichment of soil as a nitrogen fixating crop. An early onset of higher temperatures and drought are affecting chickpea and lentil growth and flowering leading to reduction of yield. In search for a tolerant varieties presented study performed a large-scale screening of two legume varieties (chickpea and lentils) investigating phenotypical response to early onset of drought under heat stress. Under heat stress and two different irrigation conditions, 19 chickpea and 18 lentil accessions were examined. The evaluation focused on their growth, biomass production, and flowering rate in comparison to commercially available varieties. Six chickpea accessions showed tolerance to water stress while only two lentil accessions differed from the rest of tested accessions. Generally, lentils genotypes were less stressed by decreased water availability compared to chickpea. Large scale screening of legume accessions could be a valuable tool to identify new varieties that could show phenotypical traits more adaptable to climate related environmental stresses. To improve the reproductive efficiency in chickpeas and lentils under adverse conditions associated to climate change an extensive breeding effort should be focused on investigation of more tolerant genotypes and cultivation in crop systems.

HIV-positive pregnant women are more vulnerable to infection and persistence of human papillomavirus (HPV). The aim of this study was to determine the prevalence and genotypes of HPV in a cohort of HIV-positive pregnant women during pregnancy and one year after delivery, analyzing risk factors for persistence. A prospective study was performed with pregnant women followed-up in the Integral Assistance Program for HIV-positive Pregnant Women of the Federal University of Rio de Janeiro, between 2009 and 2011. Demographic, clinical and laboratorial data were evaluated. Cervical samples were collected during pregnancy and one year after delivery. HPV DNA and p53 gene were amplified by polymerase chain reaction (PCR) technique and cloning. HPV direct PCR sequences were typed by submission to Blast Search tool at the National Center for Biotechnology Information (NCBI) website. The prevalence of HPV during pregnancy was 84.3% (118/140), and 106 samples were genotyped. Thirty different HPV types were founded, of which 81.1% were high-risk type. The most prevalent types were: 16, 58, 35, and 53. Infection by multiple types occurred in 18.9%. Persistent infection was noted in 50%, being 30% type-specific and 20% reinfection cases. Persistence was correlated with a TCD4+ count ≤ 350 cells/mm³ (p = 0.05). No correlation was observed between p53 polymorphism and infection by HPV or persistence of HPV infection. In conclusion, there was a high rate of HPV prevalence in this cohort of HIV-positive pregnant women, with a predominance of oncogenic types and persistence of infection in half of the cases, which was correlated with immune suppression.

Air quality monitoring data are useful in different areas of research and have varied applications, especially with a focus on the relationship between air pollution, respiratory problems, and other health hazards. The main atmospheric pollutants are: ozone (O3), sulfur dioxide (SO2), carbon monoxide (CO), nitrogen dioxide (NO2), and particulate matter (PM). PM is one of the main objects of study when one intends to protect people from exposure to pollutants. This study contributes to the analysis of PM2.5 in 21 stations in the state of São Paulo monitored by the Environmental Company of São Paulo State (CETESB). It employs cluster analysis, a prominent data mining method for detecting patterns and discovering similarities which is important for assessing air pollution, especially in a geographically vast area such as that of the state of São Paulo, which does not follow a single pattern. Another data mining technique (association rules) supports the analysis of the relationship between pollutants and meteorological variables, as it allows identifying changes between elements that occur together, in a wide variety of data. Our objectives include determining stations with similar behaviors and exploring the temporal variety of the pollutant as it relates to the dominant meteorological factors in the periods of high concentration. The clustering algorithm automatically separates stations according to their monthly averages of PM2.5 concentration between 2017 and 2019. The clusters of stations that showed the highest pollution rates essentially included urban centers with emissions by industries and vehicles, while those with the lowest rates were located further inland. A cyclical behavior in pollutant variation was also observed in the three years under study and for both clusters. For the months with the highest concentration of PM2.5, association rule learning was applied to connect air temperature, relative humidity, and wind speed with PM2.5 and carbon monoxide (CO) concentrations. The obtained results are useful to analyze the temporal and geolocation profiles of pollution by particulate matter, since they identify the behavior of the meteorological factors that predominate in periods of greater concentration.

Introduction: Percutaneous nephrolithotomy is the first therapeutic option for staghorn kidney stones. Objective: To characterize patients with complications of percutaneous nephrolithotomy for the treatment of staghorn renal lithiasis. Method: A series of 191 patients, operated by percutaneous nephrolithotomy, was studied. Variables studied: type of staghorn lithiasis, position for the technique, stone-free condition after the operation, presence of complications, time, type and grade according to the Clavien-Dindo classification. Absolute and relative frequencies were found and the chi-square test was used to determine the association between variables. Results: 86.9 % were less than 60 years old, 67,0 % were male, 61,7 % had comorbidities. The staghorn lithiasis was partial or total (30,3 % and 46,5 %, respectively). In 60,2 % it affected the left kidney; 58.1 % underwent supine surgery and 70,2 % were stone free with monotherapeutic percutaneous nephrolithotomy. Complications occurred in 19,9 %; 16,2 % were postoperative, 14,1 % infectious, 7,8 % Clavien-Dindo I, and 5,2 % IIIb. The type of lithiasis and the position of the percutaneous nephrolithotomy were not associated with complications (p> 0,05). The degree of complication was not related to the type of lithiasis (p> 0,05). Conclusions: The most frequent postoperative complications are those related to infection and bleeding; they slightly predominate in patients with partial and total staghorn stones and in those operated on in the supine position; the Clavien-Dindo grade of complications is only slightly higher in the more complex staghorn stones.

The histological diagnosis of sarcoma can be difficult as it sometimes requires the combination of morphological and immunophenotypic analyses with molecular tests. A total of 2705 tissue samples of sarcoma consecutively collected from 2006 until 2020 that had undergone molecular analysis were assessed to evaluate their diagnostic utility compared with histological assessments. A total of 3051 molecular analyses were performed, including 1484 gene fusions tested by c/qRT–PCR, 992 gene rearrangements analysed by FISH, 433 analyses of the gene status of MDM2, 126 mutational analyses and 16 NGS analysis. Of the samples analysed, 68% were from formalin-fixed, paraffin-embedded tissue and 32% were from frozen tissue. C/qRT–PCR and FISH analyses were conclusive on formalin-fixed, paraffin-embedded tissue in 74% and 76% of samples, respectively, but the combination of the two methods gave us conclusive results in 96% and 89% of frozen and formalin-fixed, paraffin-embedded tissues, respectively. We demonstrate the utility of c/qRT–PCR and FISH for sarcoma diagnosis and that each has advantages in specific contexts. We conclude that it is possible to accurately predict the sarcoma subtype using a panel of different subtype-specific FISH probes and c/qRT–PCR assays, thereby greatly facilitating the differential diagnosis of these tumours.

The collection and conservation of chickpea and lentil germplasm has great importance due to their role as a staple food for millions of people. Nowadays, the largest chickpea collection, 41.2% of the total accessions, is held by ICRISAT, while the main lentil collection is stored by ICARDA. The main fraction of both collections is constituted by Indian landraces. In recent decades, several efforts have been made to integrate all genebanks into a global conservation system of plant genetic resources. The release of informatics platforms allowed the creation of virtual genebanks, which are powerful tools routinely consulted by germplasm users. The information available for the chickpea and lentil accessions included in the Genesys and EURISCO platforms has been taken into consideration in this mini review. This review also presents and discusses the strengths and drawbacks of the worldwide collections of both species emerging from the analysis of data obtained by enquiring these two platforms. The availability of core collections is of great utility for germplasm users, while the lack of origin and biological status for a significant fraction of accessions are the main drawbacks. Moreover, this review highlights the necessity of sharing as much as possible of the non-omic and omic characterization data.

RESUMEN Introducción: Las comorbilidades se han relacionado con mayor riesgo de contraer COVID-19, de tener mala evolución y de mortalidad. Objetivo: Identificar factores de riesgo asociados a la mortalidad al final de la estadía hospitalaria de los pacientes con diagnóstico de COVID-19 en Cuba. Métodos: Investigación retrospectiva, de la totalidad de pacientes confirmados con COVID-19 en Cuba, con 18 años o más, diagnosticados entre el 11 de marzo al 15 de octubre de 2020. La fuente de información fue la base de datos nacional del Ministerio de Salud Pública. Se recopilaron edad, sexo y comorbilidades. Clasificados en recuperados y fallecidos. Estudio analítico de factores y comorbilidades asociadas a la mortalidad. Resultados: Se analizaron 5 490 pacientes (97,7 % recuperados vs 2,3 % fallecidos). Los fallecidos tenían significativamente más edad (72,8 vs 44,5 años). Las comorbilidades más frecuentes en fallecidos fueron hipertensión (47,6 % vs 7,8 %), diabetes (27,4 % vs 2,6 %), cardiopatía isquémica (14,5 % vs 0,9 %), enfermedad renal crónica (11,3 % vs 0,3 %) y EPOC (11,3 % vs 0,5 %). Se encontró riesgo independiente de mortalidad a la edad de 60 años o más (OR:10,090, IC 95 %: 6,247-16,299), enfermedad renal crónica (OR:8,434, IC 95 %: 3,400-20,919), cáncer (OR:7,169, IC 95 %: 2,920-17,601), enfermedad pulmonar obstructiva crónica (OR:5,300, IC95 %: 2,376-11,822), obesidad (OR:4,230, IC 95 %: 1,362-13,139), insuficiencia cardiaca (OR:4,197, IC 95 %: 1,085-16,244), diabetes (OR:2,360, IC 95 %: 1,339-4,159), hipertensión (OR:2,264, IC 95 %: 1,398-3,668) y cardiopatía isquémica (OR:2,321, IC 95 %: 1,168-4,610). Se encontró asociación significativa de comorbilidades, con dos, tres y más de tres (OR: 22,9, IC 95 %: 13,4-39.2), (OR:72,5, IC 95 %: 39,8-132,1) y (OR:88,9, IC 95 %: 36,2-217,8), respectivamente. La combinación de comorbilidades más frecuente entre los fallecidos fue: hipertensión con diabetes (17,7 % vs 1,9 %) e hipertensión con enfermedad renal crónica (8,1 % vs 0,2 %). Conclusiones: La edad avanzada, la enfermedad renal crónica, cardiopatía isquémica, insuficiencia cardiaca, hipertensión, diabetes, cáncer, enfermedad pulmonar obstructiva crónica, obesidad y el número de comorbilidades se relacionaron significativamente con la mortalidad y podrían ayudar a identificar a los pacientes con mayor riesgo.

ABSTRACT Introduction: Sexually transmitted infections (STI) remain a major public health problem and surveillance is crucial for prevention and control strategies. Objective: Our aim was to assess the prevalence of STI in a reference center for gynecology in Rio de Janeiro, Brazil. Materials and methods: It is a cross-sectional study conducted between August 2016 and June 2017. Whole blood and cervical cells were collected from 62 women and tested for human papillomavirus (HPV), human immunodeficiency virus (HIV), syphilis, chlamydia, gonorrhea and herpes simples virus 1 and 2 (HSV-1/2). Cervical lesions were diagnosed by cytopathology and in some patients by colposcopy (79%). Other STI were evaluated during clinical examination. Results: Cervical lesions were detected by cytopathology examinations in 46.8% of patients; those with a history of four sexual partners were at higher risk of developing them. There was moderate agreement between the cytopathology e colposcopy results (Kappa = 0.69). The prevalence of HSV (96.7%), syphilis (6.4%) and HIV (3.2%) were higher than that described in the literature, while the prevalence of chlamydia (6.4%) and gonorrhea (1.6%) were similar. HPV was detected in 53.2% of women, 32.3% of which were infected by HPV 16. In the context of co-infections, 38 women (61.3%) presented more than one STI. Conclusion: Since most of the women analyzed were affected by more than one STI, our results suggest that routine screening for these infections at health centers would help in early detection, treatment and prevention of these infections. These measures would also impact on patients’ cervical cancer control.

RESUMEN Introducción: El ácido zoledrónico mejora la calidad de vida en pacientes con metástasis óseas por cáncer prostático. Objetivo: Evaluar la calidad de vida relacionada con la salud con el cuestionario EORTC QLQ-BM22 en pacientes con metástasis óseas por cáncer prostático tratados con ácido zoledrónico. Métodos: Se realizó un estudio prospectivo-descriptivo de 71 pacientes con cáncer prostático metastásico a hueso tratados en el servicio de oncología del Hospital Clínico Quirúrgico “Hermanos Ameijeiras” con: edades 18-80 años, ECOG6 meses, y seguimiento de al menos doce meses. Se administró ácido zoledrónico cada 21-28 días. Se aplicó la escala visual análoga y el módulo EORTC QLQ-BM22. Resultados: Los pacientes tenían una mediana de 71 años de edad, Gleason ≥ 8: en 57,7 % de los pacientes, PSA al diagnóstico ≥ 20 ng/mL: 70,4 %, ECOG 1: 67,6 %, y estadio IV como presentación inicial: 50,7 %. Metástasis óseas sin toma visceral: 84,5 %, en vértebras 36,6 %,

Apulia (southeast of Italy) has always been an agricultural region in the Italian landscape. Nowadays, the countryside is a mix of natural environments and artificial landscapes. From 2014 to 2016, the region has been the object of a project aimed to collect information about geographic position and surface devoted to legume and/or cereal landrace cultivation. The collection missions carried out allowed the acquisition of 352 samples belonging to eight legumes and six cereal species. Chickpeas predominated among legumes, while durum and common wheat landraces prevailed among cereals. The cultivation of these landraces was mainly located in the marginal areas of central and southern Apulia region. The GIS technology, used in order to geo-reference the collection sites, allowed investigating the changes in land cover/land use (LCLU) occurred over the last three decades at each collecting site. In addition to the evidences collected at field level, the comparison of old ortho-photos and recent satellite images, available on Copernicus Land Monitoring Service of European Environment Agency, shown that very limited changes of LCLU have occurred. Near the collection sites, the agrarian landscape is remained highly fragmented as it was three decades ago. The cultivation side by side of irregular patches with legumes, cereals, olive trees, and grapevine characterize the agricultural landscape. Based on these evidences it can be infer that the absence of significant changes in LCLU has play a positive role in the safeguard of on farm conservation of Apulian landraces.

Introducción: La insuficiencia cardiaca es una enfermedad devastadora por su alta mortalidad. La asociación entre obesidad y mortalidad en esta enfermedad resulta polémica. Objetivo: Evaluar la influencia de la obesidad en la mortalidad de los pacientes del sexo masculino con insuficiencia cardiaca crónica. Método: Se realizó un estudio prospectivo en 109 pacientes del sexo masculino atendidos en el Hospital Hermanos Ameijeiras por un periodo de diez años. Se estimó la probabilidad de estar libre de muerte, mediante el empleo del método de Kaplan Meier. Para identificar la asociación entre las variables clínicas y de pronóstico en relación a la mortalidad se determinó el riesgo relativo (RR). Para evaluar el valor pronóstico se utilizó el modelo de regresión de Cox, se determinaron Odd Ration (OR). Resultados: La probabilidad de sobrevivir al año de seguimiento para los obesos fue 0,86 y a los diez años 0,5; los de peso normal 0,69 al año y ninguno a diez años. La presencia de fracción de eyección del ventrículo izquierdo menor a 40% se relacionó en el análisis univariado con el riesgo de muerte significativamente, p= 0,05; RR: 7,14 (IC-95%: 1,18-43,1). El riesgo proporcional de mortalidad en el tiempo para los obesos presentó un coeficiente beta de - 180 y OR: 0,835. Conclusiones: La presencia de obesidad en los pacientes del sexo masculino con insuficiencia cardiaca crónica no aumentó la mortalidad, tuvieron mejor supervivencia a cinco años.

Introducción: Se desconoce qué marcadores de riesgo nutricional estratifican mejor el riesgo en pacientes críticos. Objetivo: Evaluar el riesgo nutricional en pacientes críticos mediante dos escalas. Material y métodos: Estudio descriptivo, prospectivo, transversal, con 222 pacientes ingresados en la Terapia 8B del Hospital “Hermanos Ameijeiras” (septiembre 2017 / mayo 2018). Se utilizaron el Control Nutricional (CONUT) y el Nutrition Risk in the Critically ill modificado (mNutric). Resultados: Según CONUT el 96,4 % estaba desnutrido; según mNutric el 27,5 % eran pacientes en alto riesgo nutricional. Hubo asociación entre las escalas (Kappa = 0,102). El 78.3 % de los sobrevivientes eran bajo riesgo por mNutric (p=0,013). Hubo asociación entre la sepsis y la desnutrición por mNutric (p=0,013), no así entre la ventilación mecánica artificial (VMA) y la desnutrición estimada por dicha escala (p=0,116). No se encontraron diferencias entre la sepsis y la desnutrición según CONUT (p=0,126). Hubo diferencias entre la VMA en relación con la desnutrición según CONUT (p=0,027). La frecuencia de fallecidos se incrementó paralelo al grado de desnutrición según CONUT (p=0,004). La variable que más influyó sobre la mortalidad fue la VMA (OR= 8,5). Conclusiones: Según el CONUT, la mayoría de los pacientes estaban desnutridos, y según el mNutric, predominaron los pacientes en bajo riesgo nutricional. Se demostró el valor predictivo de muerte de la presencia de VMA. La desnutrición ligera y moderada y la variable no desnutrido de la escala CONUT, se consideraron categorías de menor riesgo de muerte con respecto a la desnutrición grave.

Introducción: La asociación entre obesidad e incremento del riesgo cardiovascular en prevención primaria está bien establecida; después que se diagnostica insuficiencia cardiaca, paradójicamente, la obesidad parece conferir una ventaja en la supervivencia y existen resultados controversiales respecto a la paradoja en subgrupos de poblaciones seleccionadas, como la diabética. Objetivo: Evaluar la influencia del índice de masa corporal en la mortalidad de pacientes diabéticos tipo 2 con insuficiencia cardiaca crónica. Método: estudio observacional de cohorte prospectivo en el periodo comprendido entre enero del año 2006 y diciembre de 2018, la muestra quedó conformada por 153 pacientes diabéticos tipo 2 con insuficiencia cardiaca crónica. Se evaluó la supervivencia, mediante el método de Kaplan-Meier. Se utilizó el modelo de regresión de Cox para estimar el efecto del índice de masa corporal sobre el pronóstico en relación a la mortalidad. Resultados: observamos que las categorías sobrepeso y obesidad tuvieron mejor supervivencia que los que presentaron bajo peso (al año 0,41, a los tres y cinco años 0,33), la curva de éstos se distanció del resto significativamente, Log Rank p= 0,000. En el modelo de regresión de Cox la variable bajo peso presentó una relación significativa en relación al tiempo en que ocurre el evento de muerte, con OR= 4,33 (intervalo de confianza de 1,69-11,09). Conclusión: En los pacientes diabéticos tipo 2 con insuficiencia cardiaca crónica se observó el fenómeno de obesidad paradójica.

Environmental degradation and the decrease of ecosystem service provision are currently of major concern, with current agricultural systems being a major driver. To meet our future environmental and sustainability targets a transformation of the agro-food systems and current agricultural value chain are crucial. One approach to redesign farming systems is the concept of biodiversity-based agriculture (BBA) which relies on sustainable diversification of biological components and their natural interactions in farming systems to maximize fertility, productivity, and resilience to external perturbations. Despite minimizing anthropogenic inputs, BBA is not yet able to meet all beneficial environmental objectives. BBA applied in the Mediterranean basin requires urgent innovation in approaches, methodologies, and models for small-holder traditional farming systems to ensure a stable provision of ecosystem services and better resilience to environmental stresses linked to climate change. Legumes are the backbone of the Mediterranean agro-ecosystems from ancient times, but their unique and wide biodiversity was not sufficiently valorized, especially by North-African countries. Here, we present LEGU-MED, a three-year international project funded by PRIMA initiative 2019. An international consortium was established involving five universities, 5 research institutes, and one private company from 8 countries: Italy, Germany, Spain, Algeria, Tunisia, Turkey, Lebanon, and Croatia. The main objective of this project is to put forward an international and well-integrated plan to valorize the legume agrobiodiversity of the Mediterranean in biodiversity-based farming systems and consequently enhance agro-ecosystem functions and services in the Mediterranean basin. The successful completion of LEGU-MED will have the following impacts on Mediterranean legume-based farming systems: (1) improve water use efficiency, (2) reduce the use of anthropogenic inputs through the maintenance of soil fertility, (3) enhance pollination and improve ecological connectivity with flora and fauna, (4) protect close-by wildland ecosystems, (5) enhance other ecosystem services (e.g., pest, disease, and weed suppression), and (6) provide healthier and safer protein-rich food.

Archaeological remains and historical documents demonstrate that a single-flower vetch has been cultivated in Italy from the early stages of agriculture. Some Italian communities have perpetuated the custom to eat its seeds still to the present. This is the case of people living in some villages of the southern Apulia region. In consequence of the high resemblance of the single-flower vetch (Vicia articulata Hornem.) seeds with those of lentils, the Apulian landrace is locally named “lenticchia nera di Soleto” (black lentil from Soleto). The evaluation of seed nutritional traits of this landrace revealed good macronutrient contents (proteins and starch, 28.4 and 42.4 g/100 g respectively), low trypsin inhibitor levels (4.08 TIU/mg), short cooking times after soaking (24–25 min) and a lack of broken seeds at the end of cooking. The coat content of total phenolic compounds (TPC) of the Apulian black lentil was comparable with that of the lentil cv. Beluga (68.23 vs. 66.14 mg GAE/g, respectively).

Existe en la actualidad un Plan Nacional de Control y Erradicación de la Tuberculosis Bovina, en el que uno de los métodos más importantes para estimar la prevalencia es a partir de la detección de animales enfermos mediante el diagnóstico anatomopatológico macroscópico en frigoríficos.Para hacer confiables estos datos, se realizan otros estudios entre los que se encuentra el histopatológico. De acuerdo con la bibliografía y trabajos anteriores de los autores, la relación de proporciones entre los diagnósticos macroscópico y microscópico variaba entre el 54 y el 90%. Esta variación era excesivamente amplia para estimar la prevalencia de la enfermedad en el ganado bovino a partir de los datos obtenidos en frigoríficos. El objetivo del presente trabajo consistió en analizar uno de los factores que inciden en la amplitud de las correlaciones halladas, aumentando el número de muestras de cada individuo sospechado de estar enfermo. Fueron confirmados por histopatología el 60,40% de las muestras estudiadas (considerando una sola submuestra), este porcentaje aumentó al 69,80% cuando se recurrió al diagnóstico de las otras submuestras.Para conocer la sensibilidad y la especificidad del diagnóstico histopatológico se decidió realizar cultivos específicos tanto en las muestras positivas como las negativas a dicho estudio. Los resultados fueron los siguientes: la sensibilidad del diagnóstico histopatológico fue del 71,15% y la especificidad del 97,77%.Este primer muestreo reveló que la prevalencia de la tuberculosis en la población estudiada y en el período analizado fue del 2,64%

os protozoos son células eucariotas simples (organismos cuyas células tienen membrana nuclear) con características del reino animal, ya que son móviles y heterótrofos. El nombre, que proviene del griego proto: primero y zoo: animal, avala la hipótesis de que son los seres vivos más antiguos, que fueron las primeras células que existieron. Debido a su tamaño pequeño y a la producción de quistes que les permiten resistir a las condiciones medioambientales adversas, muchas especies son cosmopolitas (Cairns y Ruthven, 1972), mientras que otras son de distribución limitada.

El inmunodiagnóstico en el ovino presenta problemas de sensibilidad y especificidad que limitan su aplicación en sistemas de vigilancia de la enfermedad. El Objetivo del trabajo fue desarrollar una técnica sensible, específica y económica para el diagnóstico de la echinococcosis quística en ovinos naturalmente infectados y evaluar la validez de la necropsia como prueba de referencia. 247 ovinos fueron estudiados en sala de faena confirmándose los diagnósticos parasitológicos mediante histología. Los sueros obtenidos fueron procesados mediante enzimo inmuno ensayo con tres preparaciones antigénicas: LHT (líquido hidatídico total), S2B (fracción purificada de LHT) y B y confirmados mediante western blot. EIE con las tres preparaciones antigénicas fue eficaz para discriminar E. granulosus de Cisticercos tenuícolis y Taenia spp. Sueros de ovinos negativos macroscópicamente resultaron reactivos a EIE y positivos a WB. La sensibilidad fue de 89.2% para LHT, 80.0% para S2B y 86.4% para B. La especificidad fue del 89.5% para LHT, 93.9% para S2B y 92.8% para B en el total de la majada. La sensibilidad en corderos resultó de 78.6 con LHT, 75.0 con S2B y 64.3 con B. El diagnóstico macroscópico en sala de faena demostró tener limitaciones como prueba de referencia para el inmunodiagnóstico de la equinococcosis quística en el ovino, resultando necesario incluir histología y WB como pruebas de referencia. EIE resultó una técnica con sensibilidad y especificidad adecuadas para su uso en sistemas de vigilancia y evaluación de programas de control, resultando LHT la preparación antigénica de mayor valor.

In a work developed in Argentine, La Pampa Province, Maracó Department, to proved the relationship between the microscopic and macroscopic diagnosis of bovine tuberculosis (BT) which on observed an important quantity (30,20%) that granulomatous macroscopic lesions diagnosed as tuberculosis were negative in the hystopathology diagnosis. The present work was realized to determinate what is the agents that cause this lesions. Which the 46,66% of this cases resulted actinomycosis and wooden tongue. The remainder (53,44%), were lesions compatibles with the mentionated lesions. This findings proved the importance of actinomycosis and wooden tongue as endemic disease for the studied zone

There is an increasing attention towards common bean due to its health benefits, prevention to human diseases and as ingredient for functional or fortified foods. Phosphorus, an essential element for plant growth, is mainly stored in seeds as phytic acid (Phy). Phy is negatively associated with mineral bioavailability, but, at the same time, is a natural antioxidant. Accumulation and partitioning of phosphorus were analysed in seeds of ten Italian common bean landraces for three subsequent growing seasons. Some important seed quality traits were also evaluated. For comparative purposes, the landrace harvests of two growing locations were analysed. A wide variation of total and phytic phosphorus contents was recorded among the landraces. Moreover, P accumulation and partitioning between Phy and inorganic P, as well as seed quality traits, resulted strongly affected by growing location. Statistically significant increases of Phy levels were recorded for harvests obtained outside the traditional area of cultivation. These results highlight how the cultivation of a landrace outside of its traditional area will appreciably affect harvest quality.

Se describen los criterios, el proceso metodológico y Las Fuentes de información par media de Los cuales la Facultad de Educación de la Universidad del Magdalena determino la selección de las Líneas de Investigación que fortalecen la investigación y la estrategia interna de formación de formadores de los programas de Educación de las modalidades presenciaI y a distancia.

The genetic structure of the “Badda” common bean cultivated at Polizzi Generosa, a village of Sicily (Palermo, Italy), was investigated using biochemical and molecular markers. Seed storage protein analysis by using SDS-PAGE, confirmed the attribution to the Andean gene pool. Simple Sequence Repeats (SSR) (or microsatellite) molecular markers provided useful information on genetic variation and relationships between “Badda bianco” and “Badda nero” morphotypes. Based on SSR data, the nine accessions examined were grouped in three sub-clusters. The first sub-cluster included all the accessions belonging to the “Badda bianco”. Conversely, “Badda nero” was constituted by two well-distinguished sub-clusters, one of them forming a well-separated branch. This result suggests that two constitutive nuclei contributed to the genetic background of “Badda nero”. Moreover, technological and nutritional data evidenced a good seed protein content (mean value 240.7 g kg−1) and differences in seed hydration rate among accessions. Knowledge of genetic structure appear to be fundamental in planning safeguard strategies of an appreciate landrace such as the “Badda” bean.

Introducción: el SWIFT es un índice creado para predecir eventos adversos (reingresos y mortalidad oculta) tras el alta de la Unidad de Cuidados Intensivos. Objetivo: evaluar la utilidad de la escala pronóstica SWIFT en la predicción de eventos adversos tras el alta de la unidad. Métodos: se realizó un estudio de cohorte en la Unidad de Cuidados Intensivos polivalente 8B del Hospital Clínico Quirúrgico “Hermanos Ameijeiras”, de provincia La Habana, en el periodo comprendido desde el 1ro de marzo de 2009 hasta el 28 de febrero de 2011. Los pacientes fueron divididos en dos grupos según el resultado de la escala: < 15 puntos y otro con ≥ 15, evaluándose la ocurrencia de mortalidad oculta y reingresos. Como variables principales se midieron: la mortalidad, los eventos adversos, la puntuación de la escala SAPS- 3 y la comparación de ésta con la escala SWIFT. Resultados: la escala SWIFT resultó ser útil en la predicción de eventos adversos tras el alta de UCI sin discriminar en el tiempo. La mayor puntuación de SAPS-3 al ingreso se correspondió con un mayor valor del Índice de SWIFT al egreso de la Unidad de Cuidados Intensivos. Conclusiones: los pacientes egresados de la Unidad de Cuidados Intensivos con una puntuación SWIFT ≥15 puntos presentaron una estadía discretamente superior aquellos con SWIFT‹15 puntos no teniendo relevancia estadística.

Objective. To analyze the quality of life of undergraduate students of a nursing course. Methods. This was a descriptive and cross-sectional study with a quantitative approach conducted in a public university in a municipality of Paraná State, Brazil; the study used the World Health Organization Quality of Life questionnaire (WHOQOL-BREF) of the World Health Organization. Results. The study included 95 students, predominantly young (83.2%), single (89.4%), females (91.6%), living with friends (47.4%), receiving allowances (59%), with enough monthly income (77.9%), and dedication of seven to eight daily hours to academic activities (25.2%). Among the areas of quality of life, social relationships showed the highest score (77.20) followed by psychological (67.73), environmental (64.85), and physical (63.40). The relationship between sociodemographic variables and domains of quality of life was not significant according to the Student’s t and ANOVA tests. Conclusion. This study highlights the importance of attention to the physical health of nursing students. How to cite this article: Mortz AR, Pereira EM, Borba KP, Clapis MJ, Gevert VG, Mantovani MF. Quality of life of undergraduate nursing students at a Brazilian public university. Invest. Educ. Enferm. 2016; 34(3): 564-572.

Se realizó un estudio descriptivo, retrospectivo con todos los pacientes con Bloqueo Completo de Rama Izquierda (BCRI) que se hicieron ecocardiografía de estrés con dobutamina para el diagnóstico de isquemia miocardica en el Hospital Hermanos Ameijeiras entre abril del 2004 y diciembre del 2008. La muestra estuvo conformada por 40 pacientes. Se obtuvo los resultados ecocardiográficos relacionándolos con la coronariografía.Se determinaron los indicadores de utilidad diagnóstica para el test evaluado, obteniéndose los siguientes resultados: sensibilidad 86%, especificidad 81%, precisión 85%, valor predictivo negativo 69% y positivo 91%. La movilidad regional y el umbral isquémico fueron indicadores significativamente estadísticos para el diagnóstico de isquemia. La prueba tuvo buenos resultados en la detección de enfermedad de un vaso y la descendente anterior fue la arteria con mayor prevalencia de estenosis significativa. La ecocardiografía de estrés con dobutamina resultó ser muy útil en nuestro medio para el diagnóstico de isquemia miocárdica en los pacientes con BCRI.

La investigación científica se reconoce como la forma principal que tiene la ciencia para enriquecer su acervo de conocimientos acerca de la realidad natural y social que nos rodea. Pero, si esta actividad no se realiza con la calidad necesaria se obtendrán resultados cuestionables y por tanto se corre el riesgo de que se introduzcan en la práctica acciones cuya validez no ha sido íntegramente demostrada. Esta es la justificación principal para el empleo de procedimientos metodológicos que conduzcan a realizar investigación con la calidad que permita introducir sus resultados en la práctica con la seguridad necesaria. El artículo aborda las razones para promover la calidad en la investigación y brinda elementos sobre cómo evaluarla a través de lo que se publica en revistas científicas.

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There is little information on the humoral response of sheep experimentally infected with Echinococcus granulosus. Thus, the objective of this study was to evaluate this response and measure its evolution. Doses of 10, 100, 1000 and 10000 E. granulosus eggs were prepared and inoculated via intraruminal puncture. Blood samples were obtained before inoculation and every 48 h after inoculation, until they became seropositive. Thereafter, they were taken monthly for the first year and then every three months until 1700 days of observation had been completed. An ELISA test, with total hydatid fluid antigen, was used for immunodiagnosis. The average optical density of the 12 inoculated sheep was found to be above the mean cutoff value 10 days after inoculation, went on increasing until 180 days after inoculation and remained above the cutoff level until the end of the observation period. This confirms that the antibody response of sheep to E. granulosus infection occurs before production of hydatid fluid and that activation, mobilization and establishment of oncospheres in the tissues generates a persistent response from the host's immune system.

Chickpea and lentils are one of the most important legumes not only as sources of food and nutrients but also for enrichment of soil as a nitrogen fixating crop. An early onset of higher temperatures and drought are affecting chickpea and lentil growth and flowering leading to reduction of yield. In search for a tolerant varieties presented study performed a large-scale screening of two legume varieties (chickpea and lentils) investigating phenotypical response to early onset of drought under heat stress. Under heat stress and two different irrigation conditions, 19 chickpea and 18 lentil accessions were examined. The evaluation focused on their growth, biomass production, and flowering rate in comparison to commercially available varieties. Six chickpea accessions showed tolerance to water stress while only two lentil accessions differed from the rest of tested accessions. Generally, lentils genotypes were less stressed by decreased water availability compared to chickpea. Large scale screening of legume accessions could be a valuable tool to identify new varieties that could show phenotypical traits more adaptable to climate related environmental stresses. To improve the reproductive efficiency in chickpeas and lentils under adverse conditions associated to climate change an extensive breeding effort should be focused on investigation of more tolerant genotypes and cultivation in crop systems. [ABSTRACT FROM AUTHOR]

Camelina [Camelina sativa (L.) Crantz] is an emerging oilseed crop that is attracting interest because of its high environmental adaptability and oil and protein quantity and quality. Seventeen camelina varieties of different geographical origins, sown in the same locality on two sowing dates, were compared for oil, protein, and antinutritional compounds in the meal. ANOVA showed that sowing time significantly affected the content of main fatty acids, whereas 1000-seed weight, glucosinolates (GLSs), trypsin inhibitor (TI), monounsaturated and polyunsaturated fatty acids were related to the genotype. Among the varieties analyzed, Pearl and CCE26 proved to be the best source of healthy oil, with high n-3/n-6 ratio, whereas Luna and Przybrodzka, having a lower content of GLSs and TI, were suitable for animal feed. Principal Component Analysis (PCA) allowed to group the 17 varieties into three subgroups, and the largest variation was observed among the Canadian varieties. [ABSTRACT FROM AUTHOR]

Introduction: In biliary tract exploration it is common practice to use a T-tube, however, at present there is controversy between primary choledochorraphy and T-tube placement, having studies in which it is concluded that primary choledochorraphy is a feasible option with a lower presence of complications than in T-tube placement. The objective is to compare both techniques and their postoperative evolution in the German-Nicaraguan hospital in Managua. Material and Methods: The analysis was performed by means of a descriptive study, a total of 49 patients were included, who underwent scheduled biliary tract exploration, of which primary choledochorraphy was performed in 21 patients (42.9%) and a T-tube was placed in 28 (57.1%). Result: Most of the patients were female with 86%, 11 patients (22.4%) were already cholecystectomized. Primary choledochorraphy was performed in less time, the in-hospital stay was longer in those who underwent T-tube placement, being the latter the one that presented more complications. Conclusion: Primary choledochorraphy is a safe technique, with less surgical time and better quality of life. [ABSTRACT FROM AUTHOR]