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Plasmodiumparasites contribute to one of the highest global infectious disease burden. To achieve this success, the parasite has evolved a range of specialized subcellular compartments to extensively remodel the host cell for its survival. The information to fully understand these compartments is likely hidden in the so far poorly characterized Plasmodiumspecies spatial proteome. To address this question, we have determined the steady-state subcellular location of >12,000 parasite proteins across five different species by extensive subcellular fractionation of erythrocytes infected by P. falciparum, P. knowlesi, P. yoelii, P. bergheiand P. chabaudi. This comparison of the pan-species spatial proteomes and their expression patterns indicates increasing species-specific proteins associated with the more external compartments, supporting host adaptations and post-transcriptional regulations. The spatial proteome here offers a comprehensive insight into the different human, simian, and rodent Plasmodiumspecies, establishing a powerful resource for understanding species-specific host adaptation processes in the parasite.