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Phloridzin is the most abundant polyphenolic compound in apple (Malus × domestica Borkh.), which results from the action of a key phloretin-specific UDP-2'-O-glucosyltransferase (MdPGT1). Here, we simultaneously assessed the effects of targeting MdPGT1 by conventional transgenesis and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome editing. To this end, we conducted transcriptomic and metabolic analyses of MdPGT1 RNA interference knockdown and genome-edited lines. Knockdown lines exhibited characteristic impairment of plant growth and leaf morphology, whereas genome-edited lines exhibited normal growth despite reduced foliar phloridzin. RNA-sequencing analysis identified a common core of regulated genes, involved in phenylpropanoid and flavonoid pathways. However, we identified genes and processes differentially modulated in stunted and genome-edited lines, including key transcription factors and genes involved in phytohormone signalling. Therefore, we conducted a phytohormone profiling to obtain insight into their role in the phenotypes observed. We found that salicylic and jasmonic acid were increased in dwarf lines, whereas auxin and ABA showed no correlation with the growth phenotype. Furthermore, bioactive brassinosteroids were commonly up-regulated, whereas gibberellin GA

Aim: To analyse changes in the quantity and quality of media reporting about dementia in Australian media between two time periods. Methods: A media retrieval service collected all news items related to dementia. Quality ratings based on previously developed criteria were made for a stratified random sample of items – 1129 items for 2000/2001 and 1606 for 2006/2007. Nine items of quality were assessed. A summary score for quality was constructed. The content of the sampled media items was also coded. Results: Overall, the mean total quality score for dementia-related items significantly improved over the study period. There were very large improvements in quality of reporting of ‘sensationalism’, ‘language’ and ‘provision of information about help services’ and some small deterioration in quality for ‘medical terminology’ and ‘illness versus person’. Conclusions: A very positive finding here is that generally the quality of reporting dementia has improved over the period studied.

To analyse changes in the quantity and quality of media reporting about dementia in Australian media between two time periods.A media retrieval service collected all news items related to dementia. Quality ratings based on previously developed criteria were made for a stratified random sample of items - 1129 items for 2000/2001 and 1606 for 2006/2007. Nine items of quality were assessed. A summary score for quality was constructed. The content of the sampled media items was also coded.Overall, the mean total quality score for dementia-related items significantly improved over the study period. There were very large improvements in quality of reporting of 'sensationalism', 'language' and 'provision of information about help services' and some small deterioration in quality for 'medical terminology' and 'illness versus person'.A very positive finding here is that generally the quality of reporting dementia has improved over the period studied.

The media exert a powerful influence on the public's actions, including in the sensitive area of suicide. Over the past decade, the Australian government has been sufficiently concerned about media...

There are worldwide concerns that pro-suicide web sites may trigger suicidal behaviors among vulnerable individuals. In 2006, Australia became the first country to criminalize such sites, sparking heated debate. Concerns were expressed that the law casts the criminal net too widely; inappropriately interferes with the autonomy of those who wish to die; and has jurisdictional limitations, with off-shore web sites remaining largely immune. Conversely, proponents point out that the law may limit access to domestic pro-suicide web sites, raise awareness of Internet-related suicide, mobilize community efforts to combat it, and serve as a powerful expression of societal norms about the promotion of suicidal behavior.

Aims. To evaluate changes in Australian news media reporting of suicide between 2000/01 and 2006/07 against recommendations in the resource Reporting Suicide and Mental Illness. Methods. Newspaper, television, and radio items on suicide were retrieved over two 12-month periods pre- and postintroduction of Reporting Suicide and Mental Illness. Identifying and descriptive information were extracted for each item. Quality ratings were made for a stratified random sample of items, using criteria from the precursor to Reporting Suicide and Mental Illness. Results and Conclusions. There was almost a two-fold increase in reporting of suicide during the study period, with 4,813 and 8,363 items retrieved in 2000/01 and 2006/07, respectively. The nature of media reporting showed some variability, with an increased emphasis on items about individuals’ experiences and a reduced emphasis on policy and program initiatives. Most strikingly, there was significant improvement on almost all individual dimensions of quality and overall quality. These findings are positive, although there are still clearly some opportunities for improving the way in which the media report and portray suicide. In order to improve standards, continued support should be provided for the dissemination and evaluation of Reporting Suicide and Mental Illness.

Background Media awards to encourage responsible reporting of suicide have been introduced in several countries, including Australia, Belgium and Denmark. Aims This study aimed to examine the experiences of Australian, Belgian and Danish award recipients in preparing stories on suicide, and consider the impacts of the awards for these recipients and for media professionals more broadly. Method We conducted semi-structured telephone interviews with the majority (14 out of 15) of past recipients of the awards in the three countries of interest. Results Media awards appear to show promise as a method of reinforcing national and international media guidelines on reporting suicide. The recipients of awards were proud to have had their achievements recognized in this way, and had developed a heightened awareness of the issues inherent in reporting suicide. Although relatively few had prepared subsequent stories on suicide, a number had been given opportunities to provide advice to other media professionals about how best to approach this sensitive topic. Recipients viewed the awards as an important means by which good quality reporting can be rewarded, and a springboard for raising community awareness about suicide. Conclusion The experience from Australia, Belgium and Denmark suggests that media awards which recognize responsible reporting of suicide are extremely worthwhile.

Examines competitiveness of national newspapers and how their competitiveness with regard to pagination provides a comprehensive study. Considers whether or not the newspaper industry is a declining industry, and gives many examples with circulation figures to argue against this. Sates that there is more need to understand more fully the factors interrelating with the pagination of newspapers, both in the long and short‐terms. Breaks down the production process of a newspaper with direct costs and specifications. Discusses policies, constraints, long term revenue and cost factors, plus others affecting pagination.