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Today, it is of vital importance to research actuators and its control to harness their advantages, such as the force-to-weight ratio of the actuator or its energy consumption, in a more efficient way. For this reason, this work describes the characterization and control of actuators based on Shape Memory Alloy wires with different diameters ranging from 0.5 to 2 mm. These Nickel Titanium wires contract in length when heated, which makes them a distinguishing property compared to other alloys. This contraction is used to apply force so that it can be used in applications such as moving objects, controlling valves, or designing exoskeletons, among others. In this context, the objective of this work is to characterize Shape Memory Alloy wires of varying diameters, ranging from 0.5 mm to 2 mm, which are capable of lifting loads exceeding 100 kg with a single wire, while maintaining controlled positioning. Among the parameters to be highlighted are cooling and heating times, speed, energy consumption, breaking limits, and duty cycles. Additionally, various heating methods for Shape Memory Alloys wires are analyzed: direct heating by Joule effect and external heating. The results obtained with the proposed control strategy for steady-state position control (when the output remains within ±5% of the final value) were as follows: RMSE of 0.0401% for the one-way 0.51 mm wire, 0.0483% for the two-way 0.51 mm wire, 0.289% for the 1 mm wire, and 0.0857% for the 2 mm wire.

Introduction/Purpose: Minimally invasive surgical (MIS) treatment of hallux valgus (HV) deformity is increasing in popularity. A 2mm-diameter burr is used to create a distal first metatarsal osteotomy prior to capital fragment translation and fixation. The metatarsal will shorten by the burr’s diameter (2mm). Plantar or dorsal capital fragment displacement may also cause load transference and possibly transfer metatarsalgia. In this study, we examine the effect of MIS HV on forefoot loading mechanics with respect to metatarsal shortening and sagittal plane displacement. Methods: Four lower-limb cadaveric specimens were studied. A pedobarography pressure-sensing mat was used to record forefoot plantar pressure in a controlled weightbearing stance position.10 Control and post-osteotomy measurements were obtained with the capital fragment fixated in 3 possible positions: 0mm, 5mm dorsal, and 5mm plantar displacement. Pedobarography data yielded pressure data within measurable graphical depictions. Raw mean contact pressure measurements were taken under the first and fifth metatarsal heads to establish medial and lateral forefoot loading pressure ratios. A priori power analysis was performed based on previous peer-reviewed pedobarographic data and our study was adequately powered. Results: 40 measurements were recorded and ratios of medial-to-lateral forefoot loading were constructed. Medial forefoot pressure change control versus 0mm displacement, and control versus dorsal displacement was not found to be statistically significant (p=0.525, p=0.55 respectively). Significant medial pressure increase was identified comparing control versus plantar displacement (p=0.006). Lateral pressure increased significantly with dorsal displacement of the osteotomy (p=0.013). Conclusion: MIS hallux valgus correction does not appear to cause increase in lateral forefoot pressure loading when sagittal plane displacements are controlled. Plantar displacement increases medial loading, and dorsal displacement increases lateral loading. The clinician must consider metatarsal head position post-osteotomy, as decrease in medial loading and subsequent increase in lateral loading may lead to lateral forefoot pain and transfer metatarsalgia.

Hydroponics refers to a modern set of agricultural techniques that do not require the use of natural soil for plant germination and development. These types of crops use artificial irrigation systems that, together with fuzzy control methods, allow plants to be provided with the exact amount of nutrients for optimal growth. The diffuse control begins with the sensorization of the agricultural variables that intervene in the hydroponic ecosystem, such as the environmental temperature, electrical conductivity of the nutrient solution and the temperature, humidity, and pH of the substrate. Based on this knowledge, these variables can be controlled to be within the ranges required for optimal plant growth, reducing the risk of a negative impact on the crop. This research takes, as a case study, the application of fuzzy control methods to hydroponic strawberry crops (Fragaria vesca). It is shown that, under this scheme, a greater foliage of the plants and a larger size of the fruits are obtained in comparison with natural cultivation systems in which irrigation and fertilization are carried out by default, without considering the alterations in the aforementioned variables. It is concluded that the combination of modern agricultural techniques such as hydroponics and diffuse control allow us to improve the quality of the crops and the optimization of the required resources.

Increasing economic integration and global synchronization can be key for countries aiming to catch up in GDP per capita terms. Little attention has hitherto been placed in synchronization as determinant of convergence. In this paper we estimate the effect of economic globalization and synchronization on income convergence for a sample of 89 developed and developing countries in the period 1970–2015. We use a dynamic factor model and panel data techniques to undertake the objectives of the paper. We show that synchronized countries (those correlated with the factor) exhibit a higher response on GDP per capita growth with variations on the global business cycle. This implies that synchronization improves growth for that group in global expansionary phases, but also implies risks during global recessions. On the contrary, the effect on growth of an economic globalization index is less relevant for synchronized countries than for asynchronized countries. The latter result implies that asynchronized countries can benefit more increasing their levels of economic globalization.

The Harlequin Shrimp Hymenocera picta is abundant in the Indo-Pacific and Central Pacific regions, but there are few reports of it from the eastern Pacific. Two pairs of the Harlequin Shrimp were observed feeding on the Sea Star Phataria unifascialis (Gray, 1840) in the reefs of Huatulco National Park, Mexican Pacific. This paper reports the occurrence of H. picta in Mexican Pacific waters and extends its previous distribution by 1,270km north of El Ocotal, Costa Rica in the eastern Pacific equatorial zone. In addition, we evaluate the potential distribution of H. picta along the tropical eastern Pacific using the Maximum-Entropy modelling algorithm.

Pirenaica is the most important autochthonous cattle breed within the Protected Geographic Indication (PGI) beef quality label in the Basque region, in northern Spain. The short tandem repeats (STRs) are powerful markers to elucidate forensic cases and traceability across the agri-food sector. The main objective of the present work was to study the phylogenetic relationships of Pirenaica cattle and other breeds typically raised in the region and provide the minimum number of STR markers for parentage and traceability purposes. The 30-STR panel recommended by the International Society of Animal Genetics-Food and Agriculture Organization of the United Nations (ISAG-FAO) was compared against other commercial STR panels. The 30-STR panel showed a combined matching probability of 1.89 × 10−25 and a power of exclusion for duos of 0.99998. However, commercial STR panels showed a limited efficiency for a reliable parentage analysis in Pirenaica, and at least a 21-STR panel is needed to reach a power of exclusion of 0.9999. Machine-learning analysis also demonstrated a 95% accuracy in assignments selecting the markers with the highest FST in Pirenaica individuals. Overall, the present study shows the genetic characterization of Pirenaica and its phylogeny compared with other breeds typically raised in the Basque region. Finally, a 21-STR panel with the highest FST markers is proposed for a confident parentage analysis and high traceability.

T-cell-based therapies have emerged as one of the most clinically effective ways to target solid and non-solid tumors. HER2 is responsible for the oncogenesis and treatment resistance of several human solid tumors. As a member of the HER family of tyrosine kinase receptors, its over-activity confers unfavorable clinical outcome. Targeted therapies directed at this receptor have achieved responses, although development of resistance is common. We explored a novel HER2/CD3 bispecific antibody (HER2-BsAb) platform that while preserving the anti-proliferative effects of trastuzumab, it recruits and activates non-specific circulating T-cells, promoting T cell tumor infiltration and ablating HER2(+) tumors, even when these are resistant to standard HER2-targeted therapies. Its in vitro tumor cytotoxicity, when expressed as EC50, correlated with the surface HER2 expression in a large panel of human tumor cell lines, irrespective of lineage or tumor type. HER2-BsAb-mediated cytotoxicity was relatively insensitive to PD-1/PD-L1 immune checkpoint inhibition. In four separate humanized mouse models of human breast cancer and ovarian cancer cell line xenografts, as well as human breast cancer and gastric cancer patient-derived xenografts (PDXs), HER2-BsAb was highly effective in promoting T cell infiltration and suppressing tumor growth when used in the presence of human peripheral blood mononuclear cells (PBMC) or activated T cells (ATC). The in vivo and in vitro antitumor properties of this BsAb support its further clinical development as a cancer immunotherapeutic.

Los enfoques y las metodologías aplicados hasta ahora al estudio de las desigualdades de desarrollo y bienestar entre países suelen limitarse a abordar el problema de las brechas de desarrollo a partir de la evaluación de unas pocas variables o dimensiones. El presente trabajo pretende aportar un “diagnóstico” de la situación de la Argentina hacia fines de los años 2000, pero en base a un enfoque más integral en el cual se desagrega el análisis en once áreas, buscando saber si los resultados obtenidos por el país en ellas son acordes con su nivel de ingreso per cápita. Los resultados muestran que hay todavía déficits significativos en la mayoría de esas áreas. ABSTRACT So far, the approaches and methodologies applied to the analysis of inequalities in development and well-being between countries are usually limited to address the topic of development gaps through the evaluation of only a few variables or dimensions. The present work aims to provide a “diagnosis” of Argentinean development gaps in the late 2000s, by implementing a methodology in which eleven key areas are identified in order to learn whether Argentina’s outcomes in them are aligned with the country’s GDP per capital level. Results suggest that negative gaps still exist in most of the identified areas.

Small molecules have been shown to be potent and selective probes to understand cell physiology. Here, we show that imidazo[1,2-a]pyridines and imidazo[1,2-a]pyrimidines compose a class of compounds that target essential, conserved cellular processes. Using validated chemogenomic assays in Saccharomyces cerevisiae, we discovered that two closely related compounds, an imidazo[1,2-a]pyridine and -pyrimidine that differ by a single atom, have distinctly different mechanisms of action in vivo. 2-phenyl-3-nitroso-imidazo[1,2-a]pyridine was toxic to yeast strains with defects in electron transport and mitochondrial functions and caused mitochondrial fragmentation, suggesting that compound 13 acts by disrupting mitochondria. By contrast, 2-phenyl-3-nitroso-imidazo[1,2-a]pyrimidine acted as a DNA poison, causing damage to the nuclear DNA and inducing mutagenesis. We compared compound 15 to known chemotherapeutics and found resistance required intact DNA repair pathways. Thus, subtle changes in the structure of imidazo-pyridines and -pyrimidines dramatically alter both the intracellular targeting of these compounds and their effects in vivo. Of particular interest, these different modes of action were evident in experiments on human cells, suggesting that chemical-genetic profiles obtained in yeast are recapitulated in cultured cells, indicating that our observations in yeast can: (1) be leveraged to determine mechanism of action in mammalian cells and (2) suggest novel structure-activity relationships.