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26 results on '"Andreev-Andrievskiy, Alexander"'

2. Potent painkiller from spider venom antagonizes P2X3 receptors without dysgeusia

Author

Oparin, Peter, Khokhlova, Oksana, Cherkashin, Aleksandr, Nadezhdin, Kirill, Palikov, Victor, Palikova, Yulia, Korolkova, Yuliya, Mosharova, Irina, Rogachevskaja, Olga, Baranov, Mikhail, Shaidullova, Ksenia, Ermakova, Elizaveta, Lushpa, Vladislav, Bruter, Alexandra, Deykin, Alexey, Ivanova, Elena, Silaeva, Yulia, Dyachenko, Igor, Bocharov, Eduard, Sitdikova, Guzel, Andreev-Andrievskiy, Alexander, Poteryaev, Dmitry, Shuster, Alexander, Murashev, Arkady, Kolesnikov, Stanislav, Stepanenko, Vasiliy, Grishin, Eugene, and Vassilevski, Alexander

Published
2024
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7. Aβ1-42 Accumulation Accompanies Changed Expression of Ly6/uPAR Proteins, Dysregulation of the Cholinergic System, and Degeneration of Astrocytes in the Cerebellum of Mouse Model of Early Alzheimer Disease

Author

Bychkov, Maxim L., primary, Isaev, Aizek B., additional, Andreev-Andrievskiy, Alexander A., additional, Petrov, Konstantin, additional, Paramonov, Alexander S., additional, Kirpichnikov, Mikhail P., additional, and Lyukmanova, Ekaterina N., additional

Published
2023
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14. Potent painkiller from spider venom antagonizes P2X3 receptors without dysgeusia

Author

Oparin, Peter, Khokhlova, Oksana, Cherkashin, Aleksandr, Nadezhdin, Kirill, Palikov, Victor, Palikova, Yulia, Korolkova, Yuliya, Mosharova, Irina, Rogachevskaja, Olga, Baranov, Mikhail, Shaidullova, Ksenia, Ermakova, Elizaveta, Lushpa, Vladislav, Bruter, Alexandra, Deykin, Alexey, Ivanova, Elena, Silaeva, Yulia, Dyachenko, Igor, Bocharov, Eduard, Sitdikova, Guzel, Andreev-Andrievskiy, Alexander, Poteryaev, Dmitry, Shuster, Alexander, Murashev, Arkady, Kolesnikov, Stanislav, Stepanenko, Vasiliy, Grishin, Eugene, and Vassilevski, Alexander

Abstract

P2X3 receptors are a validated molecular target in pain syndromes and chronic cough. Known P2X3 inhibitors generally suffer from poor selectivity and efficacy. Taking advantage of peptide combinatorial libraries found in venoms, we describe a P2X3 antagonist from the crab spider Thomisus onustus. This peptide potently inhibits P2X3 in the dorsal root and trigeminal ganglia neurons of rodents, as well as recombinant human P2X3, showing no effect on P2X2 or P2X2/3 receptors. PT6 presents a compact and rigid structure, and produces pronounced antinociception in animal models of inflammatory and neuropathic pain at low doses (0.01–0.1 mg/kg, s.c.). It does not show antinociceptive activity in P2rx3-knockout mice, providing further evidence in favor of its specificity. Importantly, PT6 shows no dysgeusia or ageusia effects, notoriously characteristic of small-molecule P2X3 ligands, and therefore stands out as an attractive hit for analgesic drug discovery.

Published
2025
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17. Water‐soluble variant of human Lynx1 positively modulates synaptic plasticity and ameliorates cognitive impairment associated with α7‐nAChR dysfunction

Author

Shenkarev, Zakhar O., primary, Shulepko, Mikhail A., additional, Bychkov, Maxim L., additional, Kulbatskii, Dmitrii S., additional, Shlepova, Olga V., additional, Vasilyeva, Nathalia A., additional, Andreev-Andrievskiy, Alexander A., additional, Popova, Anfisa S., additional, Lagereva, Evgeniya A., additional, Loktyushov, Eugene V., additional, Koshelev, Sergey G., additional, Thomsen, Morten S., additional, Dolgikh, Dmitry A., additional, Kozlov, Sergey A., additional, Balaban, Pavel M., additional, Kirpichnikov, Mikhail P., additional, and Lyukmanova, Ekaterina N., additional

Published
2020
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18. Scorpion toxin MeuNaTxα‐1 sensitizes primary nociceptors by selective modulation of voltage‐gated sodium channels.

Author

Cann, Marianne, Kuzmenkov, Alexey, Isensee, Jörg, Andreev‐Andrievskiy, Alexander, Peigneur, Steve, Khusainov, Georgii, Berkut, Antonina, Tytgat, Jan, Vassilevski, Alexander, and Hucho, Tim

Subjects
EXTRACELLULAR signal-regulated kinases, SENSORY neurons, SODIUM channels, NOCICEPTORS, XENOPUS laevis, SCORPIONS, PROTEIN kinases, TOXINS
Abstract

Venoms are a rich source of highly specific toxins, which allow the identification of novel therapeutic targets. We have now applied high content screening (HCS) microscopy to identify toxins that modulate pain sensitization signaling in primary sensory neurons of rat and elucidated the underlying mechanism. A set of venoms and fractions thereof were analyzed for their ability to activate type II protein kinase A (PKA‐II) and extracellular signal‐regulated kinases (ERK1/2). We identified MeuNaTxα‐1, a sodium channel‐selective scorpion α‐toxin from Mesobuthus eupeus, which affected both PKA‐II and ERK1/2. Recombinant MeuNaTxα‐1 showed identical activity to the native toxin on mammalian voltage‐gated sodium channels expressed in Xenopus laevis oocytes and induced thermal hyperalgesia in adult mice. The effect of MeuNaTxα‐1 on sensory neurons was dose‐dependent and tetrodotoxin‐sensitive. Application of inhibitors and toxin mutants with altered sodium channel selectivity demonstrated that signaling activation in sensory neurons depends on NaV1.2 isoform. Accordingly, the toxin was more potent in neurons from newborn rats, where NaV1.2 is expressed at a higher level. Our results demonstrate that HCS microscopy‐based monitoring of intracellular signaling is a novel and powerful tool to identify and characterize venoms and their toxins affecting sensory neurons. [ABSTRACT FROM AUTHOR]

Published
2021
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22. Efficacy of Mitochondrial Antioxidant Plastoquinonyl-decyl-triphenylphosphonium Bromide (SkQ1) in the Rat Model of Autoimmune Arthritis

Author

Andreev-Andrievskiy, Alexander A., primary, Kolosova, Nataliya G., additional, Stefanova, Natalia A., additional, Lovat, Maxim V., additional, Egorov, Maxim V., additional, Manskikh, Vasily N., additional, Zinovkin, Roman A., additional, Galkin, Ivan I., additional, Prikhodko, Anastasia S., additional, Skulachev, Maxim V., additional, and Lukashev, Alexander N., additional

Published
2016
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24. Mice in Bion-M 1 Space Mission: Training and Selection

Author

Andreev-Andrievskiy, Alexander, primary, Popova, Anfisa, additional, Boyle, Richard, additional, Alberts, Jeffrey, additional, Shenkman, Boris, additional, Vinogradova, Olga, additional, Dolgov, Oleg, additional, Anokhin, Konstantin, additional, Tsvirkun, Darya, additional, Soldatov, Pavel, additional, Nemirovskaya, Tatyana, additional, Ilyin, Eugeniy, additional, and Sychev, Vladimir, additional

Published
2014
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25. Scorpion toxin MeuNaTx alpha-1 sensitizes primary nociceptors by selective modulation of voltage-gated sodium channels

Author

van Cann, Marianne, Kuzmenkov, Alexey, Isensee, Jorg, Andreev-Andrievskiy, Alexander, Peigneur, Steve, Khusainov, Georgii, Berkut, Antonina, Tytgat, Jan, Vassilevski, Alexander, Hucho, Tim, van Cann, Marianne, Kuzmenkov, Alexey, Isensee, Jorg, Andreev-Andrievskiy, Alexander, Peigneur, Steve, Khusainov, Georgii, Berkut, Antonina, Tytgat, Jan, Vassilevski, Alexander, and Hucho, Tim

Abstract

Venoms are a rich source of highly specific toxins, which allow the identification of novel therapeutic targets. We have now applied high content screening (HCS) microscopy to identify toxins that modulate pain sensitization signaling in primary sensory neurons of rat and elucidated the underlying mechanism. A set of venoms and fractions thereof were analyzed for their ability to activate type II protein kinase A (PKA-II) and extracellular signal-regulated kinases (ERK1/2). We identified MeuNaTx alpha-1, a sodium channel-selective scorpion alpha-toxin from Mesobuthus eupeus, which affected both PKA-II and ERK1/2. Recombinant MeuNaTx alpha-1 showed identical activity to the native toxin on mammalian voltage-gated sodium channels expressed in Xenopus laevis oocytes and induced thermal hyperalgesia in adult mice. The effect of MeuNaTx alpha-1 on sensory neurons was dose-dependent and tetrodotoxin-sensitive. Application of inhibitors and toxin mutants with altered sodium channel selectivity demonstrated that signaling activation in sensory neurons depends on Na(V)1.2 isoform. Accordingly, the toxin was more potent in neurons from newborn rats, where Na(V)1.2 is expressed at a higher level. Our results demonstrate that HCS microscopy-based monitoring of intracellular signaling is a novel and powerful tool to identify and characterize venoms and their toxins affecting sensory neurons.

26. Scorpion toxin MeuNaTx alpha-1 sensitizes primary nociceptors by selective modulation of voltage-gated sodium channels

Author

van Cann, Marianne, Kuzmenkov, Alexey, Isensee, Jorg, Andreev-Andrievskiy, Alexander, Peigneur, Steve, Khusainov, Georgii, Berkut, Antonina, Tytgat, Jan, Vassilevski, Alexander, Hucho, Tim, van Cann, Marianne, Kuzmenkov, Alexey, Isensee, Jorg, Andreev-Andrievskiy, Alexander, Peigneur, Steve, Khusainov, Georgii, Berkut, Antonina, Tytgat, Jan, Vassilevski, Alexander, and Hucho, Tim

Abstract

Venoms are a rich source of highly specific toxins, which allow the identification of novel therapeutic targets. We have now applied high content screening (HCS) microscopy to identify toxins that modulate pain sensitization signaling in primary sensory neurons of rat and elucidated the underlying mechanism. A set of venoms and fractions thereof were analyzed for their ability to activate type II protein kinase A (PKA-II) and extracellular signal-regulated kinases (ERK1/2). We identified MeuNaTx alpha-1, a sodium channel-selective scorpion alpha-toxin from Mesobuthus eupeus, which affected both PKA-II and ERK1/2. Recombinant MeuNaTx alpha-1 showed identical activity to the native toxin on mammalian voltage-gated sodium channels expressed in Xenopus laevis oocytes and induced thermal hyperalgesia in adult mice. The effect of MeuNaTx alpha-1 on sensory neurons was dose-dependent and tetrodotoxin-sensitive. Application of inhibitors and toxin mutants with altered sodium channel selectivity demonstrated that signaling activation in sensory neurons depends on Na(V)1.2 isoform. Accordingly, the toxin was more potent in neurons from newborn rats, where Na(V)1.2 is expressed at a higher level. Our results demonstrate that HCS microscopy-based monitoring of intracellular signaling is a novel and powerful tool to identify and characterize venoms and their toxins affecting sensory neurons.

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