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Low-income communities of color in formerly redlined neighborhoods face persistent racial disparities and inequities in pollution exposure, access to transportation and safe streets, and inadequate provisions for health, safety, stable housing, clean air, education, and employment. In the process of attempting to remedy such disparities through major transportation infrastructure and access improvements, residents who are intended to benefit from expanded transportation access and options are often placed at increased risk of harmful displacement, gentrification, and environmental injustice impacts. This research project employs a case study of the International Boulevard Bus Rapid Transit (BRT) project in East Oakland, guided by Community Based Participatory Action Research (CBPAR) principles, to investigate specific traffic safety and racialized displacement impacts from the project and considers potential solutions towards protecting residents and preventing harmful byproducts of major transportation projects. In collaboration with a Community Advisory Council (CAC), a quantitative analysis of collisions along the corridor and demographic changes in select East Oakland neighborhoods was conducted. The results show evidence of increased fatality and injury collisions along the corridor at the start of construction of the BRT and after opening of the service as well as continued trends of gentrification and displacement in many East Oakland neighborhoods along the corridor. It is inconclusive with this research if there is a causal link between the BRT project and exacerbated displacement trends in nearby neighborhoods. Further qualitative research is needed to ground-truth and understand more fully the indirect land and housing market impacts of the BRT project.

Breast cancer is the most common cancer in women worldwide, and its treatment usually involves a combination of many medical procedures, including surgery, chemotherapy, radiotherapy, and hormonal therapy. One of the detrimental effects on physical function is reduced upper limb muscle strength. This study aimed to evaluate upper body strength intra-day and inter-day (test-retest) reliability using the handgrip strength test (HGS) and the bilateral isometric bench press (BIBP) and the test-retest reliability of the one repetition maximum on the bench press (BP-1RM) in breast cancer survivors (BCS). Thirty-two (52.94 ± 8.99 yrs) BCS participated in this study. The muscle strength tests were performed in two different moments, three to seven days apart. Intraclass coefficient correlation (ICC) and coefficient of variation (CV) were used to assess the reliability. Standard error of measurement (SEM), typical error of measurement (TEM), and minimally detectable change (MDC) analyses were performed. The Bland-Altman analysis was used to assess the agreement between test-retest. We found a reliability that can be described as “high” to “very high” (ICC ≥ 0.88; CV ≤ 10%) for intra-day and test-retest. SEM% and MDC% were lower than 5% and 11%, respectively, for all intra-day testing. SEM% and TEM% ranged from 3% to 11%, and MDC% ranged from 9% to 23% in the test-retest reliability. The agreement demonstrated a systematic bias ranging from 2.3% to 6.0% for all testing, and a lower systematic bias may be presented in the non-treated side assessed by HGS and BIBP. HGS, BIBP, and BP-1RM assessments are reliable for measuring upper-body muscle strength in BCS.

Over-the-air (OTA) testing and electromagnetic compatibility (EMC) measurements are widely performed in classical reverberation chambers. A less-known reverberation chamber, yet a considerably efficient environment for generating multipath conditions, is the vibrating intrinsic reverberation chamber (VIRC). This article thoroughly investigates the radio propagation channel inside the VIRC. The specific channels studied are narrowband single-input single-output (SISO) systems operating in the frequency range from 670–2740 MHz. A relevant application is OTA testing wireless baseband algorithms or modems for wireless sensor networks, specifically low-power wide-area networks such as Narrowband-IoT (NB-IoT), Long Range (LoRa) and Ultra-Narrowband (UNB). The focus is on the first- and second-order temporal and spectral channel characteristics: coherence time, Doppler spectrum, Doppler spread, frequency autocovariance function, coherence bandwidth, rejection rate of chi-squared goodness-of-fit test for Rician distribution, Rician $K$ -factor, and channel gain. Besides the impact of the frequency of operation, the investigation considers the effect of the rotational speed of the VIRC motors, two loading conditions, on the channel characteristics. An analysis of the measurement results shows that the stirring efficiency degrades while the coherence time increases at slow rotational speeds, with loading, and/or at low frequencies. Moreover, empirical models closely fitting the behavior of various investigated characteristics are proposed, for which we provide foundational physical interpretations. A further investigation is carried out to demonstrate the generality and briefly illustrate the potential usability of these models for both EMC and OTA testing.

In this paper, a methodology for developing a human walking model adapted to the individual measured by radar was proposed. The fundamental parameters used in the model are the gait parameters and physical dimensions. Empirical validation of the proposed methodology is undertaken, involving the acquisition of data using a 77-GHz FMCW radar. The data is collected from three distinct individuals walking five different trajectories with respect to the radar. Moreover, the gait parameter estimation accuracy is evaluated for the different walking trajectories of the target. The studied gait parameters are speed, step length, and step frequency. These could be estimated with mean errors up to 0.077 m/s, 9.3 cm, and 0.128 Hz for all trajectories, respectively. Nevertheless, these errors diminish to 0.022 m/s, 2.2 cm and 0.03 Hz, respectively when the targets walk in a straight trajectory aligned with the radar beam. Moreover, the feasibility of estimating body part dimensions directly from the radar data is investigated. It was found that only the total human height could be directly estimated using the employed hardware. Except for the tallest participant of 2.01 m, the height could be estimated with a mean absolute error up to 10.9 cm. Enhanced hardware configurations or the integration of machine learning techniques may improve the accuracy of body part dimension estimations.

Abstract Monoclonal antibodies (mAbs) eliminate cancer cells via various effector mechanisms including antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), which are influenced by the N-glycan structures on the Fc region of mAbs. Manipulating these glycan structures on mAbs allows for optimization of therapeutic benefits associated with effector functions. Traditional approaches such as gene deletion or overexpression often lead to only all-or-nothing changes in gene expression and fail to modulate the expression of multiple genes at defined ratios and levels. In this work, we have developed a CHO cell engineering platform enabling modulation of multiple gene expression to tailor the N-glycan profiles of mAbs for enhanced effector functions. Our platform involves a CHO targeted integration platform with two independent landing pads, allowing expression of multiple genes at two pre-determined genomic sites. By combining with internal ribosome entry site (IRES)-based polycistronic vectors, we simultaneously modulated the expression of α-mannosidase II (MANII) and chimeric β-1,4-N-acetylglucosaminyl-transferase III (cGNTIII) genes in CHO cells. This strategy enabled the production of mAbs carrying N-glycans with various levels of bisecting and non-fucosylated structures. Importantly, these engineered mAbs exhibited different degrees of effector cell activation and CDC, facilitating the identification of mAbs with optimal effector functions. This platform was demonstrated as a powerful tool for producing antibody therapeutics with tailored effector functions via precise engineering of N-glycan profiles. It holds promise for advancing the field of metabolic engineering in mammalian cells.

Abstract Background Morning rounds by an acute care surgery (ACS) service at a level one trauma center are uniquely demanding, given the fast pace, high acuity, and increased patient volume. These demands notwithstanding, communication remains integral to the success of surgical teams. Yet there are limited published curricula that address trauma inpatient communication needs. Observations at our institution confirmed that the surgical team lacked a shared mental model for communication. We hypothesized that creating a relationship-centered rounding conceptual framework model would enhance the provider-patient experience. Study design A mixed-methods approach was used for this study. A multi-pronged needs assessment was conducted. Provider communion items for Press Ganey and Hospital Consumer Assessment of Healthcare Providers and Systems (HCAHPS) surveys were used to measure patients’ expressed needs. Faculty with experience in relationship-centered communication observed morning rounds and documented demonstrated behaviors. A five-hour workshop was designed based on the identified needs. A pre-and post-course Assessment and course evaluation were conducted. Provider-related patient satisfaction items were measured six months before the course and six months after the workshop. Results Needs assessment revealed a lack of a shared communication framework and a lack of leadership skills for senior trauma residents. Barriers included: time constraints, patient load, and interruptions during rounds. The curriculum was very well received. The self-reflected behaviors that demonstrated the most dramatic change between the pre and post-workshop surveys were: I listened without interrupting; I spoke clearly and at a moderate pace; I repeated key points; and I checked that the patient understood. All these changed from being performed by 50% of respondents “about half of the time” to 100% of them “always”. Press Ganey top box likelihood to recommend (LTR) and provider-related top box items showed a trend towards improvement after implementing the training with a percentage difference of up to 20%. Conclusion The Inpatient Relationship Centered Communication Curriculum (I-RCCC) targeting senior residents and Nurse Practitioners (NP) was feasible, practical, and well-received by participants. There was a trend of an increase in LTRs and provider-specific patient satisfaction items. This curriculum will be refined based on the study results and potentially scalable to other surgical specialties.

Abstract We conducted a systematic review and meta-analysis of randomized control trials to formally assess the safety and efficacy of autologous whole cell vaccines as immunotherapies for solid tumors. Our primary safety outcome was number, and grade of adverse events. Our primary efficacy outcome was clinical responses. Secondary outcomes included survival metrics and correlative immune assays. We searched MEDLINE, Embase, and the Cochrane Central Register of Controlled Trials for studies published between 1946 and August 2020 using any autologous whole cell product in the treatment of any solid tumor. The Cochrane Randomized Controlled Trial risk of bias tool was used to assess risk of bias. Eighteen manuscripts were identified with a total of 714 patients enrolled in control and 808 in vaccine arms. In 698 patients receiving at least one dose of vaccine, treatment was well tolerated with a total of 5 grade III or higher adverse events. Clinical response was reported in a minority (n = 2, 14%) of studies. Autologous cell vaccines were associated with improved overall (HR 1.28, 95% CI 1.01–1.63) and disease-free survival (HR 1.33, 95% CI 1.05–1.67) over thirteen and ten trials respectively. Where reported, immune assays correlated well with clinical outcomes. Our results suggest that autologous whole cell vaccination is safe and efficacious in increasing survival in patients undergoing treatment for solid tumors. Registration: PROSPERO CRD42019140187.