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19 results on '"Andrade, Rafael E."'

2. Post-transplant lymphoproliferative disorders in a cohort of adult patients with a liver transplant from a reference hospital in Bogotá, Colombia

Author

Jurado, Leonardo F., Gómez-Aldana, Andrés, Tapias, Mónica, Cáceres, Daniela, Vera, Alonso, López-Panqueva, Rocío del Pilar, Andrade, Rafael E., Jurado, Leonardo F., Gómez-Aldana, Andrés, Tapias, Mónica, Cáceres, Daniela, Vera, Alonso, López-Panqueva, Rocío del Pilar, and Andrade, Rafael E.

Abstract

Introduction: The post-transplant lymphoproliferative disorders (PTLD) are characterized by an uncontrolled pathological lymphoid proliferation as a consequence of transplant immunosuppression therapy.Objective: To characterize the clinical and pathological characteristics of PTLD in a cohort of adult patients with liver transplant during a 15 year period at the Hospital Universitario Fundación Santa Fe de Bogota.Materials and methods: We conducted an observational retrospective study by searching for the PTLD cases diagnosed during the study period in the databases of the Liver Transplantation Unit and the Pathology Department. We collected the epidemiological, clinical, and pathological information and performed the corresponding statistics analyses.Results: During the research period, 572 patients were transplanted; the incidence of PTDL was 2.44%; 79% of them were man and the average age at the time of diagnosis was 62.5 years; 71% of the cases were diagnosed during the first year after the transplant and the same percentage EBV-seropositive patients. The most frequent pathological phenotype was monomorphic and the majority of tumors was detected in the hepatic hilum. The one-year survival was 50%.Conclusion: The high proportion of early cases and the high frequency of Epstein-Barr virus seropositivity both in donors and receptors drewour attention. More studies are necessary to have a better understanding of this condition in Colombia. This is the first PTLD clinical and pathological analysis in liver-transplant patients from Colombia to date., Introducción. Los trastornos linfoproliferativos después de un trasplante se caracterizan por la proliferación descontrolada de linfocitos como consecuencia del tratamiento inmunosupresor posterior a este.Objetivo. Caracterizar clínica y patológicamente los casos de trastornos linfoproliferativos después de trasplante (Post-Transplant Lymphoproliferative Disorders, PTLD) en una cohorte de pacientes adultos con trasplante de hígado atendidos a lo largo de 15 años en el Hospital Universitario Fundación Santa Fe de Bogotá.Materiales y métodos. Se hizo un estudio observacional retrospectivo a partir de la revisión de las bases de datos de la Unidad de Trasplante Hepático y del Departamento de Patología del Hospital en busca de los casos de PTLD diagnosticados durante el periodo de estudio. Se recolectó la información epidemiológica, clínica y patológica, y se adelantaron los análisis estadísticos.Resultados. Durante el periodo de estudio, hubo 572 pacientes con trasplante de hígado, la incidencia de trastornos linfoproliferativos fue de 2,44 %, el 79 % en hombres, y la edad promedio en el momento del diagnóstico fue de 62,5 años. El 71 % de los casos se presentó durante los primeros 12 meses después del trasplante y el mismo porcentaje fue seropositivo para el virus de Epstein-Barr (EBV). El fenotipo patológico más frecuente fue el monomorfo y la mayoría de los tumores se detectaron en el hilio hepático. La supervivencia al año fue del 50 %.Conclusiones. Llamó la atención el alto porcentaje de casos de presentación temprana, así como la gran frecuencia de seropositividad para el EBV tanto en los donantes como en los receptores. Deben adelantarse estudios más detallados para una mejor comprensión de esta enfermedad en el país. Este es el primer análisis clínico y patológico de PTLD en pacientes con trasplante de hígado adelantado en Colombia hasta la fecha.

Published
2020

7. Multicenter, Randomized Clinical Trial to Assess the Effectiveness of Intravitreal Injections of Bevacizumab, Triamcinolone, or Their Combination in the Treatment of Diabetic Macular Edema

Author

Neto, Hermelino O., primary, Regatieri, Caio V., additional, Nobrega, Mário J., additional, Muccioli, Cristina, additional, Casella, Antonio M., additional, Andrade, Rafael E., additional, Maia, Mauricio, additional, Kniggendorf, Vinicius, additional, Ferreira, Magno, additional, Branco, André C., additional, and Belfort, Rubens, additional

Published
2017
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10. Determination of the HER2 amplification status by in situ fluorescent hybridization and concordance with immunohistochemistry for breast cancer samples in Colombia

Author

Plata, Adriana, Torres, Maria Mercedes, López, Rocío, and Andrade, Rafael E

Subjects
breast cancer, FISH, cáncer de seno, HER2, Immunohistochemistry, Inmunohistoquímica
Abstract

Objectives: To determine the status of the HER2 amplification in Breast cancer performed in peripheral laboratories in Colombia by immunohistochemistry and its comparison with central laboratories and the FISH status. Methods: Four thousand one hundred and five cases referred for the determination of the HER2 status by FISH and/or IHQ to the Department of Pathology of the Fundacion Santa Fe were studied. The analysis included correlation between the IHQ HER2 score submitted by the peripheral laboratory (PL), the HER2 score emitted in the CL and the FISH studies performed in the central laboratory (CL). Results: Two thousand five hundred and eight HER2 IHQ studies were performed in the (CL), using the Dako Herceptest. With the following results: 68,2 % negative (0-1+); 16,4% indeterminate (2+); 15,3% 3+ and 2,3 % not adequate. 1360/ 1719 cases studied by FISH came from the (PL), and 329 (19,1%) from the (CL). Comparing the IHQ score emitted by the PL and the positive FISH status showed: 6/28 0+ were positive (21, 4%); 7/31 1+ (22, 5%); 397/1240 2+ (32, 8%) and 74/91 3+ (81, 3%). In the CL the results were 1/9 0+ (11,1%); 3/18 1+ (16,7%); 154/292 2+ (53%); and 9/9 3+ (100%). Only 1/4 negative cases (0/1+) was in house. Conclusion: The false negative rate (22%), and false positive results (18,7%), of the HER2 status performed by IHQ in peripheral laboratories in Colombia is unacceptable high as well as the inadequacy of tissue indicating that pre-analytical factors have to be improved in Colombia in order to get optimal results. Objetivos: Determinar la correlación entre el estado de amplificación del oncogén HER2 en cáncer de seno por inmunohistoquímica y FISH, en laboratorios periféricos y en un laboratorio central de referencia en Colombia. Métodos: Se estudiaron 4105 casos referidos al Departamento de Patología de la Fundación Santa fe de Bogotá para la determinación del estado de amplificación del HER2 por FISH y/o IHQ. El análisis incluyó la correlación entre los scores del HER2 por IHQ en ambos laboratorios y los estudios de FISH realizados en el laboratorio central. Resultados: Dos mil quinientos ocho casos fueron estudiados para HER2 por IHQ en el LC (Herceptest de DAKO); 68.2% fueron negativos (score de 0-1+); 16.4% indeterminados (2+) y 15,3% positivos (3+); 2,3% fueron inadecuados. 1360 de 1689 casos estudiados por FISH (80.5%) provenían de LP, y 329 (19.5%) del LC. En los LP se encontró amplificación por FISH en: 6/28 casos catalogados por IHQ como 0+ (21.4%); 7/31 1+ (22.5%) ; 397/1210 2+ (2.8%) y 74/91 3+ (81.3%). En el LC se encontró amplificación por FISH en 1/9 casos catalogados por IHQ como 0+ (11.1%); 3/18 1+ (16.7%), 154/292 2+ (53%); y 10/10 3+ (100%) . Un de los 4 casos falsos negativos fue procesado en su totalidad en el LC. Conclusión: Los resultados falsos negativos (22.0%) y falsos positivos (18.7%) en el estado del HER2 determinado por IHQ en los LP son inaceptablemente altos en Colombia, indicando se requiere establecer mecanismos estrictos de control de calidad.

Published
2013

19. Post-transplant lymphoproliferative disorders in a cohort of adult patients with a liver transplant from a reference hospital in Bogotá, Colombia

Author

Jurado LF, Gómez-Aldana A, Tapias M, Cáceres D, Vera A, López-Panqueva RDP, and Andrade RE

Subjects
Aged, Colombia epidemiology, Female, Herpesvirus 4, Human isolation & purification, Hospitals, University, Humans, Incidence, Liver Transplantation statistics & numerical data, Lymphoproliferative Disorders epidemiology, Lymphoproliferative Disorders pathology, Lymphoproliferative Disorders virology, Male, Middle Aged, Postoperative Complications epidemiology, Postoperative Complications pathology, Postoperative Complications virology, Retrospective Studies, Risk Factors, Sex Distribution, Immunosuppression Therapy adverse effects, Liver Transplantation adverse effects, Lymphoproliferative Disorders etiology, Postoperative Complications etiology
Abstract

Introduction: The post-transplant lymphoproliferative disorders (PTLD) are characterized by an uncontrolled pathological lymphoid proliferation as a consequence of transplant immunosuppression therapy. Objective: To characterize the clinical and pathological characteristics of PTLD in a cohort of adult patients with liver transplant during a 15 year period at the Hospital Universitario Fundación Santa Fe de Bogota. Materials and methods: We conducted an observational retrospective study by searching for the PTLD cases diagnosed during the study period in the databases of the Liver Transplantation Unit and the Pathology Department. We collected the epidemiological, clinical, and pathological information and performed the corresponding statistics analyses. Results: During the research period, 572 patients were transplanted; the incidence of PTDL was 2.44%; 79% of them were man and the average age at the time of diagnosis was 62.5 years; 71% of the cases were diagnosed during the first year after the transplant and the same percentage EBV-seropositive patients. The most frequent pathological phenotype was monomorphic and the majority of tumors was detected in the hepatic hilum. The one-year survival was 50%. Conclusion: The high proportion of early cases and the high frequency of Epstein-Barr virus seropositivity both in donors and receptors drewour attention. More studies are necessary to have a better understanding of this condition in Colombia. This is the first PTLD clinical and pathological analysis in liver-transplant patients from Colombia to date.

Published
2020
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