97 results on '"Andrac-Meyer, L."'
Search Results
2. Diagnostic différentiel entre cicatrices chéloïdes et hypertrophiques : une nouvelle approche en tomographie par cohérence optique plein-champ
- Author
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Eraud, J., Gonnelli, D., Carmassi, M., Bruzzese, L., Andrac-Meyer, L., Casanova, D., and Magalon, G.
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- 2014
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3. Mise au point d’un modèle animal original de cicatrice chéloïde
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Philandrianos, C., Gonnelli, D., Andrac-Meyer, L., Bruno, M., Magalon, G., and Mordon, S.
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- 2014
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4. Tumores benignos de los tejidos blandos de la piel
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Koeppel, M.-C. and Andrac-Meyer, L.
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- 2006
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5. Early curettage of giant congenital naevi in children
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CASANOVA, D., BARDOT, J., ANDRAC-MEYER, L., and MAGALON, G.
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- 1998
6. Plaidoyer en faveur de la greffe adipocytaire dans la technique de S.R. Coleman
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Jauffret, J.L, Champsaur, P, Robaglia-Schlupp, A, Andrac-Meyer, L, and Magalon, G
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- 2001
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7. Pyoderma gangrenosum révélant une diverticulite colique : deux cas
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Fongue, J., Brajon, D., Visée, C., Combes, E., Andrac-Meyer, L., and Berbis, P.
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- 2015
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8. c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays
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Charpin-Taranger C, Claude Allasia, Charafe-Jauffret E, David Jérémie Birnbaum, Séverine Carpentier-Meunier, Jean-Philippe Dales, Pascal Bonnier, Lavaut Mn, Stéphane Garcia, Andrac-Meyer L, and Jocelyne Jacquemier
- Subjects
Cancer Research ,medicine.medical_specialty ,Pathology ,C-Met ,Immunocytochemistry ,Breast Neoplasms ,Biology ,chemistry.chemical_compound ,Automation ,Phosphatidylinositol 3-Kinases ,Breast cancer ,immunocytochemistry ,image analysis ,medicine ,skin and connective tissue diseases ,Molecular Diagnostics ,c-Met ,Tissue microarray ,tissue microarray ,Anatomical pathology ,Proto-Oncogene Proteins c-met ,medicine.disease ,Cadherins ,Immunohistochemistry ,Oncology ,chemistry ,Tissue Array Analysis ,Cancer research ,Breast carcinoma ,Inflammatory Breast Carcinoma - Abstract
Inflammatory breast carcinoma (IBC) is a rare but aggressive tumour associated with poor outcome owing to early metastases. Increased expression of c-Met protein correlates with reduced survival and high metastatic risk in human cancers including breast carcinomas and is targetable by specific drugs, that could potentially improve the prognosis. In the present study, we compared c-Met expression in IBC (n=41) and non-IBC (n=480) immunohistochemically (Ventana Benchmark autostainer) in two tissue microarrays (TMA) along with PI3K and E-cadherin. The results were quantified through an automated image analysis device (SAMBA Technologies). We observed that (i) c-Met was significantly overexpressed in IBC as compared with non-IBC (P
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- 2007
9. Dermatose neutrophilique diffuse sévère associée à la prise d’amoxicilline-acide clavulanique : première observation
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Bonnet, N., primary, Morel, F., additional, Koeppel, M.-C., additional, Brajon, D., additional, Andrac-Meyer, L., additional, Villeret, J., additional, Default, A., additional, Jean-Pastor, M.-J., additional, and Berbis, P., additional
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- 2013
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10. Tumeurs kystiques rétropéritonéales compliquées révélatrices d’une lymphangioléiomyomatose
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Barbier, L., primary, Ebbo, M., additional, Andrac-Meyer, L., additional, Schneilitz, N., additional, Le Treut, Y.-P., additional, Reynaud-Gaubert, M., additional, and Hardwigsen, J., additional
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- 2009
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11. c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays
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Garcia, S, primary, Dalès, J-P, additional, Jacquemier, J, additional, Charafe-Jauffret, E, additional, Birnbaum, D, additional, Andrac-Meyer, L, additional, Lavaut, M-N, additional, Allasia, C, additional, Carpentier-Meunier, S, additional, Bonnier, P, additional, and Charpin-Taranger, C, additional
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- 2007
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12. Tumeurs cutanées bénignes des tissus mous
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Koeppel, M.-C., primary and Andrac-Meyer, L., additional
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- 2006
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13. P17 - Toxidermies psoriasiformes induites par l’infliximab (Remicade®) : effet variable du changement d’anti-TNFδ
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Bayle, S., primary, Koeppel, M., additional, Sportich, S., additional, Zambelli, L., additional, Dales, J., additional, Andrac-Meyer, L., additional, Roudier, J., additional, Grimaud, J., additional, and Berbis, P., additional
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- 2005
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14. P19 - Toxidermie lichénoïde au loflazépate d’éthyle (Victan®) : première observation
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Bonnet, N., primary, Sportich, S., additional, Zambelli, L., additional, Koeppel, M., additional, Dales, J., additional, Andrac-Meyer, L., additional, Thoreau, H., additional, Jean-Pastor, M., additional, and Berbis, P., additional
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- 2005
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15. P15 - Toxidermie à la darbépoiétine alpha (érythropoiétine de synthèse) : une complication exceptionnelle
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Aubran, C., primary, Zambelli, L., additional, Sportich, S., additional, Andrac-Meyer, L., additional, Dales, J., additional, Koeppel, M., additional, and Berbis, P., additional
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- 2005
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16. La surexpression immunohistochimique de HIF-1alpha est un marqueur de mauvais pronostic (suivi supérieur à 13 ans) dans le cancer du sein (n = 745 patientes)
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Dales, J.P., primary, Garcia, S., additional, Carpentier-Meunier, S., additional, Andrac-Meyer, L., additional, Lavaut, M.N., additional, Allasia, C., additional, Bonnier, P., additional, and Taranger-Charpin, C., additional
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- 2004
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17. Diagnostic difficile d’un liposarcome infraclinique responsable d’une fièvre au long cours : « petite tumeur, grande inflammation »
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Granel, B, primary, Serratrice, J, additional, Andrac-Meyer, L, additional, Ene, N, additional, Rodriguez, F, additional, Bonardel, G, additional, Champsaur, P, additional, Disdier, P, additional, and Weiller, P.-J, additional
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- 2003
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18. Intérêt des immunoglobulines intraveineusesdans le traitement du pemphigus
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Lemordant, P., primary, Koeppel, M.C., additional, Dales, J.P., additional, Andrac-Meyer, L., additional, Chouquet, D., additional, and Berbis, P., additional
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- 2003
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19. Lupus érythémateux pustuleux : À propos d'un cas spectaculaire révélant le diagnostic
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Giaime, P., primary, Mezi, L., additional, Koeppel, M.C., additional, Dales, J.P., additional, Andrac-Meyer, L., additional, and Berbis, P., additional
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- 2003
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20. Pachydermatoglyphie paranéoplasique révélatriced'un carcinome épidermo de de la base de la langue
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Lightburn, E., primary, Devant, O., additional, Dalès, J.P., additional, Andrac-Meyer, L., additional, and Berbis, P., additional
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- 2003
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21. Sarcoidosis Preceding Chronic Myelomonocytic Leukemia
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Serratrice, J., primary, Granel, B., additional, Swiader, L., additional, Disdier, P., additional, Weiller-Merli, C., additional, Lepidi, H., additional, de Roux-Serratrice, C., additional, Andrac-Meyer, L., additional, and Weiller, P.J., additional
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- 2002
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22. Atteinte hypophysaire : une manifestation des lymphomes endovasculaires
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Mazodier, K., primary, Bernit, E., additional, Faure, V., additional, Rovery, C., additional, Gayet, S., additional, Charbonnier, A., additional, Andrac-Meyer, L., additional, Horschowski, N., additional, Kaplanski, G., additional, Schleinitz, N., additional, Veit, V., additional, and Harlé, J.R., additional
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- 2001
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23. Desmoid tumour of the breast.
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Terrier, J P, primary, Garcia, S, additional, Bessenay, F, additional, Sappa, P, additional, Andrac-Meyer, L, additional, Bonnier, P, additional, and Charpin, C, additional
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- 1998
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24. Conditions of wound healing and cutaneous growth affect metabolic performance of skin following plastic surgery.
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Lutz NW, Confort-Gouny S, Casanova D, Andrac-Meyer L, Magalon G, and Cozzone PJ
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- 2007
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25. Clinicopathologic Growth Factors in Vestibular Schwannomas: a Morphological and Immunohistochemical Study of 69 Tumours.
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Labit-Bouvier, C., Crebassa, B., Bouvier, C., Andrac-Meyer, L., Magnan, J., and Charpin, C.
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ACOUSTIC neuroma ,PATHOLOGY - Abstract
Tumour growth of vestibular schwannomas is still difficult to predict. The aim of our study was to determine whether any defined histopathological feature was correlated with the clinical course. We did a retrospective study with 69 paraffin-embedded tumours to establish whether the number of vessels, blood cells extravasation or degree of inflammation, all semi-quantitatively assessed, could be indicative of potential of growth. An immunohistochemical study was also performed with an endothelial marker CD34, the leukocyte common antigen CD45 and the estrogen and progesterone hormone receptors. All these parameters were correlated with patient's age, duration of symptoms (d), with a clinical growth index (CLI = tumour size/d). No clinical parameters proved to be predictive of tumour growth. Tumour size was significantly (p = 0.01) related to the number of vessels and we found a significant relationship between the clinical growth index (CLI) and total number of vessels, especially when duration of symptoms lasted less than 1 year (p < 0.001). However, we found no relationship between duration of symptoms or CLI and CD34 index. The degree of inflammation was significantly correlated (p = 0.007) with duration of symptoms when it lasted more than 1 year. The CD45 index and the semi-quantitative evaluation of the inflammation were well correlated (p = 0.001). No estrogen receptors antigenic site was detected and only seven tumours expressed progesterone receptor in a few cells without any significant clinical value. These results suggest that vessel density is determinant for sporadic acoustic neuroma growth especially for a short clinical course. [ABSTRACT FROM AUTHOR]
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- 2000
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26. P19 - Toxidermie lichénoïde au loflazépate d’éthyle (Victan ®) : première observation
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Bonnet, N., Sportich, S., Zambelli, L., Koeppel, M., Dales, J., Andrac-Meyer, L., Thoreau, H., Jean-Pastor, M., and Berbis, P.
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- 2005
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27. P17 - Toxidermies psoriasiformes induites par l’infliximab (Remicade ®) : effet variable du changement d’anti-TNF δ
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Bayle, S., Koeppel, M., Sportich, S., Zambelli, L., Dales, J., Andrac-Meyer, L., Roudier, J., Grimaud, J., and Berbis, P.
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- 2005
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28. Difficult diagnosis of fever of unknown origin related to an infraclinic liposarcoma: “small tumor and fever of unknown origin”
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Granel, B., Serratrice, J., Andrac-Meyer, L., Ene, N., Rodriguez, F., Bonardel, G., Champsaur, P., Disdier, P., and Weiller, P.-J.
- Subjects
- *
FEVER , *PALPATION , *PHYSICAL diagnosis , *DISEASES in women , *INFLAMMATION - Abstract
Introduction. – Palpation of soft tissues constitutes part of the physical examination when faced with a patient with fever of unknown origin. Our case highlights the difficulty of diagnosis when the tumor still remains impalpable ten months after onset of fever and severe biological inflammatory process.Exegesis. – A 49-year-old woman was admitted for fever of unknown origin associated with important biological inflammatory process. In view of her past medical history of breast carcinoma, a search for a relapse was performed and remained negative. Despite multiple investigations and repeated physical examinations, no diagnosis was obtained. Finally, [(18)F] fluorodoexyglucose positron emission tomography led to a diagnosis showing a high fixation located in the right thigh, related to a myxoid liposarcoma after surgical resection.Conclusion. – Dramatic regression of fever and biological inflammatory process after surgical treatment illustrates the concept of “small tumor and fever of unknown origin”, an exceptional entity but which physicians should be aware of. The role of new technical imaging with [(18)F] fluorodoexyglucose positron emission tomography has however to be clarified in the evaluation of fever of unknown origin. [Copyright &y& Elsevier]
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- 2003
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29. Treatment of keloid scars with a 1210-nm diode laser in an animal model.
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Philandrianos C, Bertrand B, Andrac-Meyer L, Magalon G, Casanova D, Kerfant N, and Mordon S
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- Animals, Female, Humans, Keloid pathology, Mice, Mice, Nude, Treatment Outcome, Wound Healing, Keloid surgery, Lasers, Semiconductor therapeutic use
- Abstract
Background and Objective: A temperature increase can improve wound healing by activation of heat shock protein 70 and stimulation of fibroblasts. Since keloids are a dysfunction of collagen fiber synthesis and organization, this study aimed to evaluate if a 1,210 nm diode laser could have effects in a new animal model of keloid scars., Study Design/materials and Methods: A total of 39 nude mice were used for this study. Phototypes IV and V human keloids were grafted into their backs and after 1 month of healing, the mice were divided into four groups: Control, Laser, Resection, Resection/Laser. In the Laser group, the keloids were treated with a 1,210-nm diode-laser with the following parameters: 4 W; 10 seconds; fluence: 51 J/cm(2) ; spot: 18.9 × 3.7 mm(2) . In the Resection group, surgical intra-lesional excision was performed. In the Resection/Laser group, keloids were treated with the 1,210-nm laser-diode after surgical intra-lesional excision. Temperature measurements were made during the laser treatment. Clinical examination and histological study were performed on the day of treatment and 1 month, 2 months, and 3 months later., Results: Mean temperature measurement was of 44.8°C (42-48°) in the Laser groups. No healing complications or keloid proliferation was observed in any group. Keloid histologic characters were confirmed in all grafts. No histologic particularity was observed in the laser groups in comparison with the Control and Resection groups., Conclusion: First, this keloid animal model appears to be adapted for laser study. Secondly, the 1,210-nm diode laser does not induce keloid thermal damage in vivo. Further studies with different 1,210-nm laser diode parameters should be performed in order to observe significant effects on keloids., (© 2015 Wiley Periodicals, Inc.)
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- 2015
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30. [Keloid scars: a case series study].
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Carmassi M, Eraud J, Gonelli D, Magalon G, and Andrac Meyer L
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- Adolescent, Adult, Aged, Child, Cicatrix, Hypertrophic pathology, Cicatrix, Hypertrophic surgery, Female, Humans, Keloid pathology, Male, Middle Aged, Retrospective Studies, Young Adult, Keloid surgery
- Abstract
Keloids scars are a pathological way of wound healing of the human skin. They are responsible for an unsightly and functional discomfort, and recurrence is common after surgery. There are specific treatments in order to lower this risk. They can be hard to distinguish from an hypertrophic scar on clinical and histological examination. Moreover, mixed forms exist, combining keloid and hypertrophic features. We retrospectively reviewed 52 patients who underwent surgery for keloid scars. On 54 scars, histological examination found 38 keloids, 8 hypertrophic, 7 mixed scars and 2 neither keloid nor hypertrophic. Mean age was 30 years. Sex ratio was 1.2 women per 1 man. Most frequent locations were ear (44.4%), thorax (33.3%), and shoulder (7.4%). In all cases, disease duration exceeded one year. Histology help to make the definitive diagnosis of keloid, hypertrophic and mixed scar, confirmed by trichrome or orcein staining and alpha smooth muscle actin immunostaining., (Copyright © 2014 Elsevier Masson SAS. All rights reserved.)
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- 2015
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31. New fat-derived products for treating skin-induced lesions of scleroderma in nude mice.
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Serratrice N, Bruzzese L, Magalon J, Véran J, Giraudo L, Aboudou H, Ould-Ali D, Nguyen PS, Bausset O, Daumas A, Casanova D, Granel B, Andrac-Meyer L, Sabatier F, and Magalon G
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- Animals, Cells, Cultured, Female, Humans, Mice, Mice, Nude, Neovascularization, Physiologic, Sclerosis therapy, Skin blood supply, Adipose Tissue cytology, Mesenchymal Stem Cell Transplantation, Platelet Transfusion, Skin pathology, Skin Diseases therapy
- Abstract
Introduction: Scleroderma is characterized by cutaneous manifestations that mainly affect the hands, arms and face. As of today, there is no treatment for fibrotic skin lesions of scleroderma. Previously we generated and validated a model of scleroderma-like skin sclerosis in nude mice, appropriate to inject human derived products. We showed that the subcutaneous injection of micro-fat (MF), purified and injected using small caliber cannulas, have anti-fibrotic and pro-angiogenic effects and appears more suitable for the treatment of skin lesions of scleroderma compared to the gold standard (Coleman's technique or macro-fat). Here we compared the long-term efficacy of micro-fat "enriched" with other therapeutic products including the stromal vascular fraction (SVF) of fat and platelet-rich plasma (PRP) from blood in our murine model of scleroderma., Methods: We used 72 nude mice in this study. We formed six experimental groups: Macro-fat, MF, SVF, PRP, MF + SVF, MF + PRP. This project has three phases: i) Induction of skin sclerosis by daily subcutaneous injections of bleomycin (BLM) for 4 weeks in nude mice; ii) Purification and injection of the different cell therapy products; iii) Histological analyses done 8 weeks post-injections., Results: MF + SVF and MF + PRP significantly reversed dermal and epidermal sclerosis (P <0.01). Macro-fat, SVF, PRP only corrected the dermal sclerosis (P <0.05). Epidermal sclerosis was reduced in treatments containing MF (P <0.01). MF was more stable. Products containing the SVF were associated with a significant increase of the local vascularization (P <0.01)., Conclusions: All tested substances were effective in treating skin-induced lesions of scleroderma with different levels of fibrosis and vascular improvement; MF derived products are more stable and SVF demonstrated better pro-angiogenic effects. The observed efficacy of this combination of products in the animal model provides a rationale for potential clinical applications to treat human disease.
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- 2014
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32. [Establishment of a keloid model by transplanting human keloid onto the backs of nude mice].
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Philandrianos C, Gonnelli D, Andrac-Meyer L, Bruno M, Magalon G, and Mordon S
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- Animals, Female, Humans, Mice, Mice, Nude, Disease Models, Animal, Keloid surgery, Transplantation, Heterologous
- Abstract
Keloid scar is a proliferative healing dysfunction formed by an excessive build-up of collagen fibers on the dermis. It is responsible of aesthetic and functional disabilities. There is no ideal treatment and recurrence occurs very often. Keloid scars occur only to human, that's why animal model needs to be made to study this pathology and new treatments. Few models have been described using human keloid scars implanted into subcutaneous tissue of nude mice or rat. To allow study of topical and laser treatment we have developed a new animal model using human keloid scar fragment with epidermal and dermal tissue implanted into back of nude mice like a full thickness skin graft. Keloid fragments from five donors have been grafted onto 40 nudes mice. Macroscopic and microscopic studies have been made at day 28, 56, 84 and 112. We observed integration of the fragments in all cases. Hyalinized collagen bundles were observed in all implant biopsies confirming the stability of the keloid architecture within 112 days. This model is easily reproducible and allows the study of topical treatment and laser due to the accessibility of the keloid., (Copyright © 2012. Published by Elsevier Masson SAS.)
- Published
- 2014
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33. Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model.
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Philandrianos C, Andrac-Meyer L, Mordon S, Feuerstein JM, Sabatier F, Veran J, Magalon G, and Casanova D
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- Animals, Cells, Cultured, Cicatrix pathology, Epithelial Cells transplantation, Female, Random Allocation, Skin pathology, Swine, Transplantation, Autologous, Dermatologic Surgical Procedures, Skin injuries, Skin Transplantation methods, Skin, Artificial, Wound Healing
- Abstract
The wound healing attributes of five acellular dermal skin substitutes were compared, in a two-step procedure, in a porcine model. Ten pigs were included in this experimental and randomized study. During the first step, dermal substitutes (Integra(®), ProDerm(®), Renoskin(®), Matriderm(®) 2mm and Hyalomatrix(®) PA) were implanted into full-thickness skin wounds and the epidermis was reconstructed during a second step procedure at day 21 using autologous split-thickness skin graft or cultured epithelial autograft. Seven pigs were followed-up for 2 months and 3 pigs for 6 months. Dermal substitute incorporation, epidermal graft takes, wound contraction and Vancouver scale were assessed, and histological study of the wounds was performed. Results showed significant differences between groups in dermis incorporation and in early wound contraction, but there was no difference in wound contraction and in Vancouver scale after 2 and 6 months of healing. We conclude there was no long-term difference of scar qualities in our study between the different artificial dermis. More, there was no difference between artificial dermis and the control group. This study makes us ask questions about the benefit of artificial dermis used in a two-step procedure., (Copyright © 2012 Elsevier Ltd and ISBI. All rights reserved.)
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- 2012
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34. [Abdominal cystic tumor revealing lymphangioleiomyomatosis].
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Barbier L, Ebbo M, Andrac-Meyer L, Schneilitz N, Le Treut YP, Reynaud-Gaubert M, and Hardwigsen J
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- Abdominal Pain etiology, Adult, Female, Humans, Lymphangioma, Cystic surgery, Mediastinal Neoplasms surgery, Retroperitoneal Neoplasms surgery, Lymphangioleiomyomatosis diagnosis, Lymphangioma, Cystic etiology, Mediastinal Neoplasms etiology, Retroperitoneal Neoplasms etiology
- Abstract
We report the case of a 39 year-old woman with many years of intermittent abdominal pain who was found to have cystic masses evocative of cystic lymphangioma involving the posterior mediastinal and retroperitoneum. Worsening abdominal pain led to a recommendation for laparoscopic unroofing and decompression of the cysts. During the postoperative period, hemorrhagic shock required reintervention with excision of the tumoral mass. Pathologic examination revealed lymphangioleiomyomatosis (LAM). On the 15th postoperative day, the patient developed a chylopneumothorax which required prolonged chest tube drainage. The presence of multiple polycystic lesions in the pulmonary parenchyma supported the diagnosis of diffuse LAM with primary extrapulmonary presentation. This diagnosis should be considered preoperatively since it modifies the treatment: a complete excision of the cystic lesions seems to be necessary in order to prevent bleeding and lymphatic extravasation.
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- 2009
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35. Cutaneous pseudolymphoma associated with a TNF-alpha inhibitor treatment: etanercept.
- Author
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Guis S, Schiano de Colella JM, Bonnet N, Andrac-Meyer L, Balandraud N, Mattei JP, Franck B, Roudier C, Roudier J, and Berbis P
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- Etanercept, Humans, Male, Middle Aged, Receptors, Tumor Necrosis Factor, Antirheumatic Agents adverse effects, Arthritis, Rheumatoid drug therapy, Immunoglobulin G adverse effects, Pseudolymphoma chemically induced, Skin Diseases chemically induced, Tumor Necrosis Factor-alpha antagonists & inhibitors
- Published
- 2008
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36. Overexpression of c-Met and of the transducers PI3K, FAK and JAK in breast carcinomas correlates with shorter survival and neoangiogenesis.
- Author
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Garcia S, Dales JP, Charafe-Jauffret E, Carpentier-Meunier S, Andrac-Meyer L, Jacquemier J, Andonian C, Lavaut MN, Allasia C, Bonnier P, and Charpin C
- Subjects
- Breast Neoplasms chemistry, CD146 Antigen analysis, CD146 Antigen metabolism, Carcinoma chemistry, Focal Adhesion Kinase 1 metabolism, Humans, Immunohistochemistry, Janus Kinases metabolism, Neovascularization, Pathologic metabolism, Phosphatidylinositol 3-Kinases metabolism, Prognosis, Proto-Oncogene Proteins c-met metabolism, Signal Transduction, Tissue Array Analysis, Up-Regulation, Breast Neoplasms blood supply, Breast Neoplasms mortality, Carcinoma blood supply, Carcinoma mortality, Focal Adhesion Kinase 1 analysis, Janus Kinases analysis, Phosphatidylinositol 3-Kinases analysis, Proto-Oncogene Proteins c-met analysis
- Abstract
c-Met is responsible for cell motility and tumour spreading. c-Met expression and signal transducers reflecting c-Met functionality were investigated in breast carcinomas, in correlation with patient outcome and tumour vasculature. Tissue microarrays of 930 breast carcinomas were constructed, categorised according to patients' follow-up (4- to 10-year follow-up; median, 6.5 years). Standardised immunocytochemical procedures were performed using anti-c-Met, -PI3K, -FAK, -JAK, and -CD146, -FYN and an automated autostainer (Ventana). High-throughput densitometry measuring the extent of immunoprecipitates was assessed by image analysis (SAMBA). c-Met overexpression correlated with poor survival along with PI3K and FAK reflecting c-Met functionality and CD146 and FYN expression in endothelial cells. Automated quantification of immunocytochemical precipitates using image analysis was shown to provide an objective means of measuring cellular proteins that are potentially relevant for current practice in pathological diagnosis and for specific therapy combining inhibitors of both c-Met and downstream transducer pathways, and of tumour angiogenesis.
- Published
- 2007
37. Ear canal keratinocyte culture: clinical perspective.
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Sanjuan M, Sabatier F, Andrac-Meyer L, Lavieille JP, and Magnan J
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- 3T3 Cells, Adult, Aged, Animals, Child, Epidermis transplantation, Female, Fibroblasts physiology, Humans, Immunohistochemistry, Keratinocytes transplantation, Keratins biosynthesis, Male, Mice, Middle Aged, Paraffin Embedding, Tissue Fixation, Cytological Techniques, Ear Canal cytology, Keratinocytes physiology
- Abstract
Hypothesis: Autologous epidermal sheets obtained by cultivating keratinocytes of the external auditory meatus can be used to repair cutaneous defects of the ear canal. The Rheinwald and Green method has been used to know whether the produced epidermal layer preserves its specificities after the culture., Background: Using a split-thickness skin graft during a functional ear atresia surgery does not allow for the restitution of external auditory canal self-cleaning. Some authors cultivated external auditory meatus keratinocytes and showed migration capacities of these colonies., Methods: Samples of preauricular skin and of the bony part of the external auditory canal were harvested from 10 patients. Keratinocytes were extracted and cultured until an epidermal sheet was obtained. The output, the keratinocyte plating efficiency, and the production delay were measured during the culture. Culture product sections and biopsy sections were examined using optical microscopy after standard coloration and indirect immunohistochemistry., Results: Nine epidermal layers from 10 biopsies were obtained in each group. A significant difference between external auditory meatus and preauricular keratinocyte plating efficiency was highlighted. The average production delay of 23 cm2 external auditory canal and preauricular epidermal layers was 21 days. There was no difference in the cytokeratine expression between external auditory canal and preauricular skin, nor between external auditory canal and preauricular culture products. All cultures expressed the cytokeratine 5 characteristic of stratifying epithelium., Conclusion: The Rheinwald and Green keratinocyte culture method allows the production of ear canal-stratified epidermal sheets, which can be used for external ear reconstruction.
- Published
- 2007
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38. Poor prognosis in breast carcinomas correlates with increased expression of targetable CD146 and c-Met and with proteomic basal-like phenotype.
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Garcia S, Dalès JP, Charafe-Jauffret E, Carpentier-Meunier S, Andrac-Meyer L, Jacquemier J, Andonian C, Lavaut MN, Allasia C, Bonnier P, and Charpin C
- Subjects
- Adult, Aged, Aged, 80 and over, Biomarkers, Tumor analysis, Breast Neoplasms mortality, Female, Focal Adhesion Kinase 1 biosynthesis, Humans, Image Processing, Computer-Assisted, Immunohistochemistry, Immunoprecipitation, Middle Aged, Phenotype, Phosphatidylinositol 3-Kinases biosynthesis, Prognosis, Proteomics, Proto-Oncogene Proteins c-fyn biosynthesis, Tissue Array Analysis, Breast Neoplasms metabolism, Breast Neoplasms pathology, CD146 Antigen biosynthesis, Neoplasms, Basal Cell metabolism, Proto-Oncogene Proteins c-met biosynthesis
- Abstract
Genomic studies have led to new taxonomic classifications of breast carcinomas. Proteomic investigations using tissue microarrays have yielded complementary results and are useful in identifying potential molecular targets for specific therapies. Searching for new drug targets is particularly important for tumors of poor prognosis, such as breast tumors that lack estrogen receptors and HER2 amplification; in these tumors, certain molecules probably play a significant role in tumor spreading through the stromal microvasculature. We investigated 930 breast carcinomas categorized according to patients' survival (range of follow-up = 4-10 years; median follow-up = 6.5 years) using (1) automated immunohistochemical procedures (Ventana, Cedex, France) with tissue microarrays (Alphelys, Plaisir, France) and (2) quantification of immunoprecipitates assessed by automated image analysis densitometry (SAMBA, Meylan, France). Expression of c-Met and CD146 and that of signaling transducers PI3K, FAK, and FYN were compared in living and deceased patients. Expression of some proteins recently reported to be characteristic of basal cell carcinomas was also assessed, namely, CK5-6, caveolin-1, carbonic anhydrase IX, p63, and CD117; these also constitute potential targets for therapies for aggressive tumors. Overexpression of these proteins was observed in deceased or metastatic patients (P < .01 to P < .00001), particularly node-negative patients (except for FYN, p63, and CD146). c-Met and CD146 are involved in tumor spreading, and our results suggest that they probably play an important role in patients' death, along with other proteins involved in hypoxia (carbonic anhydrase IX) and other cell functions or structures (caveolin-1, CD117, CK5-6, and p63) that are expressed in an aggressive subtype of basal cell carcinoma for which no specific therapy is available.
- Published
- 2007
- Full Text
- View/download PDF
39. [Organized cervical cancer screening for underpriviledged women].
- Author
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Piana L, Leandri FX, Jacqueme B, Heid P, Corti J, Andrac-Meyer L, and Sancho-Garnier H
- Subjects
- Adult, Aged, Female, France epidemiology, Humans, Mass Media, Middle Aged, Papanicolaou Test, Registries, Socioeconomic Factors, Uterine Cervical Neoplasms pathology, Vaginal Smears, Mass Screening organization & administration, Poverty, Uterine Cervical Neoplasms epidemiology, Uterine Cervical Neoplasms prevention & control
- Abstract
Individual cervical screening with pap-smears is the major cause of the decrease in incidence and mortality of cervical cancer in France since more than 30 years. But, for the last ten years, the decrease in mortality is limited due to the persistence of poor prognostic cases and the insufficiency of treatment efficacy in such patients. These cases are mostly observed in women who did not participate to regular screening. They are generally from low socioeconomic levels or migrant populations, or both. Such an observation leads us to organize 3 successive pilot campaigns (2001, 2003, 2005) in the Northern part of Marseille city where the rate of such poor population is high (37 to 45%). The women without a pap-smear indexed in the National Insurance Register for the last 3-2 years were invited by individual mailing to perform, free of charge, a pap-smear. The evaluation of each campaign helps us to improve the next one. The major changes from the 1st one to the 3rd one were to increase the number of free screening services, to send a second invitation to the non responders, and to organize local meetings with social workers and nurses to explain to the women the importance of performing regular screening tests. The final results after the 3 campaigns showed : out of the eligible women the participation rates are dramatically low evolving from 1,56 % to 2,48% and 6,87% of pap-smears done along the 3 campaigns ; the selection of women "without pap-smear" from the national Insurance register was not good, a great number of missing data was identified, explaining partly the extremely low rate of participation ; the factors increasing a little the participation are the second mailing of invitation (39 % of pap-smears realised) ; the total gratuitous of the screening (both sampling and reading) ; the oral information delivered locally by social workers and the proximity of the places to perform the test ; the quality of the pap-smears collected by gynecologists was good but insufficient for other health professionals, requiring a specific training for cervical cell collection. The organization of such pilot projects need to be followed to find better solutions to increase the participation to cervical screening of such populations who are at high risk of poor prognosis cervical cancers.
- Published
- 2007
40. [High-throughput quantification of tissue microarrays: identification of candidate target proteins in inflammatory breast cancer].
- Author
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Taranger-Charpin C, Andrac-Meyer L, Dales JP, Carpentier-Meunier S, Andonian C, Lavaut MN, Allasia C, and Bonnier P
- Subjects
- Adenocarcinoma metabolism, Adenocarcinoma mortality, Adenocarcinoma pathology, Adult, Biopsy, Breast pathology, Breast Neoplasms metabolism, Breast Neoplasms mortality, Breast Neoplasms pathology, Cadherins metabolism, Densitometry, Female, Focal Adhesion Kinase 1, Humans, Immunohistochemistry, Phenotype, Adenocarcinoma genetics, Breast Neoplasms genetics, Proto-Oncogene Proteins c-met metabolism, Tissue Array Analysis
- Abstract
Inflammatory breast carcinoma (IBC) is a rare but very aggressive tumour phenotype. Increased c-Met protein expression correlates with reduced survival and a higher metastatic risk in many human malignancies, including breast cancer Several studies have shown that c-Met protein is targetable by specific drugs. Here we compared c-Met expression in IBC (n = 41) and non IBC (n = 480). Two microarrays of IBC and non IBC tissues were constructed and standardized. C-Met, P13K and E-cadherin were immunodetected (Ven-tana Benchmark Autostainer) on serial sections. The results were quantified with an automated image analysis device (SAMBA Technologies) by immunoprecipitate densitometry of each core section (0.6 microns thick). We found that (i) c-Met is significantly overexpressed in IBC compared to non IBC (p < 0. 001), (ii) P13K is also overexpressed (p < 0.001) in IBC, suggesting that overexpressed c-Met is functionally active, at least through the PI3K signal transduction pathway ; and (iii) E-cadherin is paradoxically overexpressed in IBC. We conclude that c-Met may constitute a target for specific therapy in patients with poor-prognosis malignancies like IBC Automated image analysis of TMA is a valuable tool for high-throughput quantification of the immunohistochemical expression of the tumor proteome.
- Published
- 2007
41. [An unusual perianal abscess].
- Author
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Soulier M, Andrac-Meyer L, Berdah S, Garcia S, Meunier-Carpentier S, and Taranger-Charpin C
- Subjects
- Abscess diagnosis, Anus Diseases diagnosis, Diagnosis, Differential, Female, Humans, Middle Aged, Myxoma diagnosis, Pelvic Neoplasms diagnosis, Treatment Outcome, Abscess surgery, Anus Diseases surgery, Myxoma surgery, Pelvic Neoplasms surgery, Perineum
- Abstract
Aggressive angiomyxoma (AA) is a mesenchymal tumour occurring in connective tissue of the perineum or lower pelvis with a marked tendency to local recurrence but which usually does not metastasize. Only 130 cases had been reported to date. We report the case of a 58-year-old woman, presenting with a pelvi-perineal mass, which was considered to be an anal abscess. After surgical excision, an AA was diagnosed, with classical histological features (myxoid and vascular components) and which was positive for vimentin and CD34. This case report shows that clinical diagnosis of AA is difficult and that delayed diagnosis can prevent optimal treatment of these tumors.
- Published
- 2007
- Full Text
- View/download PDF
42. Overexpression of hypoxia-inducible factor HIF-1alpha predicts early relapse in breast cancer: retrospective study in a series of 745 patients.
- Author
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Dales JP, Garcia S, Meunier-Carpentier S, Andrac-Meyer L, Haddad O, Lavaut MN, Allasia C, Bonnier P, and Charpin C
- Subjects
- Adult, Aged, Breast Neoplasms pathology, Female, Humans, Hypoxia-Inducible Factor 1, Hypoxia-Inducible Factor 1, alpha Subunit, Immunohistochemistry, Middle Aged, Neoplasm Metastasis, Neoplasm Recurrence, Local, Prognosis, Retrospective Studies, Breast Neoplasms chemistry, DNA-Binding Proteins analysis, Nuclear Proteins analysis, Transcription Factors analysis
- Abstract
Hypoxia-inducible factor-1alpha (HIF-1alpha) is a transcription factor that is involved in tumour growth and metastasis by regulating genes involved in response to hypoxia. HIF-1alpha protein overexpression has been shown in a variety of human cancers, but only 2 studies have documented the prognostic relevance of HIF-1alpha expression in breast cancer. The aim of our study was to determine accurately the impact of HIF-1alpha expression on prognosis in a large series (n = 745) of unselected patients with invasive breast cancer in terms of overall survival, local recurrence and distant metastasis risk. HIF-1alpha expression was investigated using immunohistochemical assays on frozen sections, and correlated with patients' outcome (median follow-up = 13.5 years). Univariate (Kaplan-Meier) analysis showed that high levels of HIF-1alpha expression (cutoff = 10%) significantly correlated with poor overall survival (p = 0.019). HIF-1alpha expression correlated with high metastasis risk among the whole group of patients (p = 0.008). Multivariate analysis (Cox model) showed that the HIF-1alpha predictive value was independent of other current prognostic indicators. Moreover among node negative ones, HIF-1alpha expression was also significantly predictive of metastasis risk (p = 0.03) and of relapse (p = 0.035). All the data suggest that HIF-1alpha is associated with a worse prognosis in patients with invasive breast carcinoma. Furthermore HIF-1alpha immunodetection may be considered as a potential indicator for selecting patients who could benefit from specific therapies interfering with HIF-1alpha pathway., ((c) 2005 Wiley-Liss, Inc.)
- Published
- 2005
- Full Text
- View/download PDF
43. [A tumor-like lymphocytis mastitis].
- Author
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Giusiano S, Andrac-Meyer L, Meunier-Carpentier S, Xerri L, Boubli L, and Taranger-Charpin C
- Subjects
- Adult, Breast Neoplasms pathology, Diagnosis, Differential, Female, Humans, Inflammation, Mastitis surgery, Necrosis, Lymphocytes pathology, Mastitis pathology
- Abstract
A 44-year-old woman presenting with an inflammatory and palpable firm breast lump underwent surgical excision. Intraoperative frozen section analysis showed an extensive lesion consisting of ducts with intraluminal "necrosis". In addition, a very dense stromal inflammation was observed around these ducts, suggesting an invasive ductal carcinoma with predominant intraductal proliferation. However, on paraffin sections, epithelial cells close to the lymphocytic infiltrate were rare, subatrophic, without any neoplastic feature. The density and architecture of the lymphocytic infiltrate mimicked a breast lymphoma. However, immunochemistry and molecular biology investigation favored the diagnosis of a tumor-like lymphocytic mastitis. Although extremely rare, this particular form of lymphocytic mastitis, a diagnostic pitfall particularly during peroperative examination, should be recognized by pathologists.
- Published
- 2005
- Full Text
- View/download PDF
44. Comparison of the prognosis indication of VEGFR-1 and VEGFR-2 and Tie2 receptor expression in breast carcinoma.
- Author
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Meunier-Carpentier S, Dales JP, Djemli A, Garcia S, Bonnier P, Andrac-Meyer L, Lavaut MN, Allasia C, and Charpin C
- Subjects
- Adult, Aged, Antibodies, Monoclonal immunology, Breast Neoplasms immunology, Female, Humans, Immunohistochemistry, Middle Aged, Multivariate Analysis, Prognosis, Survival Analysis, Breast Neoplasms pathology, Gene Expression Profiling, Neoplasm Metastasis, Neovascularization, Pathologic, Receptor, TIE-2 biosynthesis, Vascular Endothelial Growth Factor Receptor-1 biosynthesis, Vascular Endothelial Growth Factor Receptor-2 biosynthesis
- Abstract
The degree of angiogenesis in breast cancer has previously been shown to be an indicator of prognosis, and tumor microvasculature is a candidate target for new antiangiogenic therapies. The aim of this study was to investigate the prognostic value of vascular endothelial growth factor (VEGF) receptors, VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1), and Tie2/tek receptor tyrosine kinase in breast carcinoma. VEGF receptors and Tie2 expression was investigated using immunohistochemical assays with monoclonal antibodies on frozen sections in a series of 918 and 909 patients respectively. VEGFR-1 and VEGFR-2 and Tie2 were correlated with long-term (median, 11.3 years) patients' outcome. Univariate (Kaplan-Meier) analysis showed that VEGFR-1 positive tumor surface (cutoff = 5%) was significantly correlated with high metastasis risk (p=0.03) and relapse (p<0.01) in all patients, and in those with node negative tumors (p<0.001 and p<0.01 respectively), but not with overall survival. In contrast Tie2 positive tumor surface (cutoff = 7%) was significantly correlated with poor overall survival (p=0.025) and also with high metastasis risk particularly among node negative patients (p<0.01). Moreover, Tie2 immunoexpression was significantly predictive of relapse (p=0.003) in the node negative subgroup (p=0.02). In multivariate analysis (Cox model), VEGFR-1 and Tie2 immunoexpressions were identified as independent prognostic indicators. In contrast, univariate analysis showed that VEGFR-2 positive tumor surface (cutoff = 10%) was not correlated with survival or with metastasis and relapse risk. Our results suggest that VEGFR-1 and Tie2 immunohistochemical expression permits the identification of patients with poor outcome, and particularly node negative ones with a high risk for metastasis and relapse. VEGFR-1 and Tie2 immunodetection may also be considered as potential tools for selecting patients who could benefit in the future from specific antiangiogenic therapy interfering with VEGFR-1 and Tie2 activation pathways.
- Published
- 2005
45. [A calcified subcutaneous tumor].
- Author
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Marcy M, Andrac-Meyer L, Dales JP, Carpentier-Meunier S, Legré R, and Taranger-Charpin C
- Subjects
- Adult, Histocytochemistry, Humans, Necrosis, Osteoblasts pathology, Calcinosis pathology, Osteosarcoma pathology, Soft Tissue Neoplasms pathology
- Published
- 2005
- Full Text
- View/download PDF
46. [Plasmablastic lymphoma in a patient with HIV infection: an unusual case located in the skin].
- Author
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Dales JP, Harket A, Bagnères D, Andrac-Meyer L, Xerri L, Frances Y, and Taranger-Charpin C
- Subjects
- Fatal Outcome, Humans, Immunophenotyping, Lymphoma, AIDS-Related complications, Lymphoma, AIDS-Related pathology, Lymphoma, Large B-Cell, Diffuse complications, Lymphoma, Large B-Cell, Diffuse diagnosis, Lymphoma, Large B-Cell, Diffuse pathology, Male, Middle Aged, Skin Neoplasms complications, Skin Neoplasms pathology, HIV Seropositivity complications, Lymphoma, AIDS-Related diagnosis, Plasma Cells, Skin Neoplasms diagnosis
- Abstract
We report the case of a plasmablastic lymphoma involving the skin in a 45 year-old HIV-positive patient. Plasmablastic lymphoma was first described in 1997 and is considered to be a diffuse large B-cell lymphoma with a unique immunophenotype and a predilection for the oral cavity. In this case, the tumor was revealed by multiple purple cutaneous nodules predominantly localized on the trunk and proximal parts of the limbs. A skin biopsy led to the diagnosis of plasmablastic lymphoma in view of the presence of a dense nodular infiltrate invading the dermis and subcutaneous fat composed of large cells that expressed neither the leucocyte common antigen nor the B- and T-cell antigens CD20 and CD3, but which showed a strong immunostaining with plasma cell marker VS38c. Most of the cells expressed Kappa light chain of immunoglobulins, they did not express Lambda light chain. In situ hybridization with EBER probe revealed detection of Epstein Barr virus in about 15 % of tumor cells. The clinical course was aggressive and rapidly fatal. Despite one cycle chemotherapy the patient died four months after presentation. HIV-associated plasmablastic lymphoma is a poor prognosis malignancy that may resist typing due to the lack of expression of commonly used lymphoid markers.
- Published
- 2005
- Full Text
- View/download PDF
47. [Accuracy of intraoperative frozen section diagnosis in non palpable breast lesions: a series of 791 cases].
- Author
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Dorel-LeTheo M, Dales JP, Garcia S, Ramuz O, Andrac-Meyer L, Bonnier P, Piana L, and Charpin-Taranger C
- Subjects
- Breast Neoplasms diagnostic imaging, Carcinoma, Ductal, Breast diagnostic imaging, Diagnosis, Differential, Female, Humans, Mammography, Paraffin Embedding, Reproducibility of Results, Sensitivity and Specificity, Specimen Handling, Breast pathology, Breast Neoplasms pathology, Carcinoma, Ductal, Breast pathology, Frozen Sections
- Abstract
The aim of this study is to estimate the accuracy and reliability of intraoperative frozen section of nonpalpable breast lesions. In fact, frozen section of palpable breast lesions has proven to be valid, but its use in breast infraclinic lesions has been discussed recently, with the publication of European recommendations. Diagnosis on frozen section was routinely performed in a serie of 791 patients with nonpalpable mammographically detected abnormalities breast lesions from January 1990 through July 2000. The initial frozen section diagnoses with known mammographic pattern were compared with the diagnoses obtained on permanent paraffin sections to estimate the accuracy of frozen sections. Frozen section diagnosis was delayed until final diagnosis assessed on permanent paraffin sections in only 8 cases (1%). Frozen section diagnoses were accurate in 744 of 783 cases (95%). The diagnosis was modified on the basis of permanent sections in 39 cases; consisting of 39 false negative. No false positive diagnosis was noted. Sensitivity and specificity of frozen section diagnoses were 87,69 and 100, respectively. When the comparison between frozen and permanent section was analyzed according to the mammographic pattern, the sensitivity among patients with microcalcifications was lower (75,23) than among patients with opacities (93,86). When frozen section and permanent section diagnoses were related according to the mammographic size ( 10 mm) the sensitivity among patients with opacities measuring less than 10 mm was lower (91,75) than among patients with opacities larger than 10 mm (95,65) and the sensitivity among patients with microcalcifications larger than 10 mm was greater (77,14) than among patients with microcalcifications size less than 10 mm (74,28). This results are similar to those obtained on palpable breast lesions, and show that frozen section is a feasible and reliable procedure in nonpalpable breast lesions, particularly more relevant in mammographic opacities than in microcalcifications, whatever the lesion size.
- Published
- 2003
48. CD105 expression is a marker of high metastatic risk and poor outcome in breast carcinomas. Correlations between immunohistochemical analysis and long-term follow-up in a series of 929 patients.
- Author
-
Dales JP, Garcia S, Bonnier P, Duffaud F, Andrac-Meyer L, Ramuz O, Lavaut MN, Allasia C, and Charpin C
- Subjects
- Adult, Aged, Aged, 80 and over, Antigens, CD, Breast blood supply, Breast pathology, Breast Neoplasms mortality, Breast Neoplasms pathology, Carcinoma mortality, Carcinoma secondary, Endoglin, Female, Follow-Up Studies, Humans, Immunoenzyme Techniques, Microcirculation pathology, Middle Aged, Proportional Hazards Models, Receptors, Cell Surface, Survival Analysis, Survival Rate, Biomarkers, Tumor metabolism, Breast Neoplasms metabolism, Carcinoma metabolism, Vascular Cell Adhesion Molecule-1 metabolism
- Abstract
CD105 (endoglin) is expressed significantly in activated endothelial cells in culture and in tumor microvessels. Quantification of CD105 immunocytochemical expression that may be clinically relevant has not been accurately evaluated. We studied CD105 expression on frozen tissue sections by using immunohistochemical assays in a series of 929 patients and correlated the findings with long-term follow-up (median, 11.3 years). Univariate (Kaplan-Meier) analysis showed that the number of CD105+ microvessels (cutoff, 15 vessels) correlated significantly with poor overall survival among all patients (P = .001). This correlation was less significant in node-negative patients (P = .035). Marked CD105 expression also correlated with a high risk for metastasis among all patients (P = .006) and among node-negative patients (P = .001). Multivariate analysis (Cox model) identified CD105 immunodetection as an independent prognostic indicator. Our results suggest that immunohistochemical expression of CD105 has practical clinical relevance for identifying node-negative patients with a poor prognosis. Moreover, immunodetection of CD105 also may be considered a potential tool for selecting patients who could benefit from specific antiangiogenic therapy, using anti-CD105 conjugates.
- Published
- 2003
- Full Text
- View/download PDF
49. Tie2/Tek expression in breast carcinoma: correlations of immunohistochemical assays and long-term follow-up in a series of 909 patients.
- Author
-
Dales JP, Garcia S, Bonnier P, Duffaud F, Meunier-Carpentier S, Andrac-Meyer L, Lavaut MN, Allasia C, and Charpin C
- Subjects
- Adult, Aged, Biomarkers, Tumor physiology, Breast Neoplasms mortality, Carcinoma mortality, Female, Follow-Up Studies, Frozen Sections, Humans, Immunoenzyme Techniques, Life Tables, Middle Aged, Neoplasm Metastasis, Neoplasm Proteins physiology, Prognosis, Proportional Hazards Models, Receptor Protein-Tyrosine Kinases physiology, Receptor, TIE-2, Risk, Survival Analysis, Biomarkers, Tumor analysis, Breast Neoplasms enzymology, Carcinoma enzymology, Neoplasm Proteins analysis, Neovascularization, Pathologic metabolism, Receptor Protein-Tyrosine Kinases analysis
- Abstract
The degree of angiogenesis in breast cancer has previously been shown to be an indicator of prognosis, and tumor microvasculature is at present a candidate target for new antiangiogenic therapies. Tie2/tek receptor tyrosine kinase is a novel marker of microvasculature of solid tumors that appears to play a key role in the angiogenesis process in breast cancer. However the prognostic significance of Tie2 has never been demonstrated in this neoplasm. In order to establish the prognostic value of Tie2 in breast carcinoma, we investigated Tie2 expression in a large series of patients and correlated it with long-term follow-up. Tie2 expression was investigated using immunohistochemical assays with a polyclonal antibody on frozen sections in a series of 909 patients, and was correlated with long-term (median, 11.3 years) follow-up. Univariate (Kaplan-Meier) analysis showed that a large Tie2 positive tumor surface (cut off = 7%) was significantly correlated with poor overall survival (p=0.025). Tie2 expression correlated with high metastasis risk among all patients (p=0.00067) and among node negative ones as well (p=0.01). Tie2 immuno-expression was also significantly predictive of relapse in all patients (p=0.003) and in the node negative subgroup (p=0.02). In multivariate analysis (Cox model) Tie2 immunodetection was identified as an independent prognostic indicator. Our results suggest that Tie2 immunohistochemical expression exhibits practical clinical relevance in terms of prognostic prediction. Tie2 expression permits identification of poor outcome patients, in particular node negative ones with high risk of metastasis and relapse. Tie2 immunodetection may further be considered as a potential tool for selecting patients who could benefit in the future from specific antiangiogenic therapy interfering with Tie2 pathway.
- Published
- 2003
50. [The immunohistochemical expression of CD105 is a marker for high metastatic risk and worse prognosis in breast cancers].
- Author
-
Charpin-Taranger C, Dales JP, Garcia S, Andrac-Meyer L, Ramuz O, Carpentier-Meunier S, and Bonnier P
- Subjects
- Adult, Aged, Aged, 80 and over, Antigens, CD, Breast Neoplasms blood supply, Breast Neoplasms mortality, Breast Neoplasms pathology, Endoglin, Endothelium, Vascular chemistry, Endothelium, Vascular pathology, Female, Follow-Up Studies, Humans, Life Tables, Lymphatic Metastasis, Middle Aged, Neoplasm Metastasis, Neovascularization, Pathologic metabolism, Prognosis, Proportional Hazards Models, Receptors, Cell Surface, Risk, Survival Analysis, Biomarkers, Tumor analysis, Breast Neoplasms chemistry, Neoplasm Proteins analysis, Vascular Cell Adhesion Molecule-1 analysis
- Abstract
The quantification of angiogenesis in human solid tumors has been shown to be an indicator of prognosis and tumor microvasculature is a candidate target for antiangiogenic therapy. CD105 (endoglin) is significantly expressed in activated endothelial cells in culture and in tumor microvessels. Quantification of CD105 immunocytochemical expression that may be of significant clinical relevance, has not been accurately evaluated as yet. In the present report, CD105 expression on frozen sections was investigated using immunohistochemical assays in a series of 929 patients and correlated with long-term (median = 11.3 years) follow-up. The CD105 immunostaining was observed on endothelial cells mostly in small cells. The number of vessels and the immunostained surface were evaluated in so called "hot spots" within tumor stroma. Both the number of vessels and immunostained surface were correlated to the patients' outcome (overall survival, disease free survival, metastases) in the whole group of patients and also specifically in node negative subgroup. Univariate (Kaplan Meier) analysis showed that the number of CD105 positive microvessels (cut-off n = 15) was significantly correlated with poor overall survival, among all patients (p = 0.001). This correlation was less significant in the group of node negative patients (p = 0.035). Marked CD105 expression was also correlated with high metastasis risk among all patients (p = 0.006) and among node negative patients as well (p = 0.001). In multivariate analysis (Cox model) CD105 immunodetection was identified as an independent prognostic indicator. Our results suggest that CD105 immunohistochemical expression has a practical clinical relevance for identifying node negative patients with poor prognosis. Moreover, the CD105 immunodetection may also be considered as a potential tool for selecting patients that could benefit from specific antiangiogenic therapy, using anti CD105 conjugates.
- Published
- 2003
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