Search

The experimental study aimed to induce the acute alithiasic cholecystitis in 40 pigs, injecting L-α-Phosphatidylcholine in the gallbladder using laparoscopy and to establish the role of Diclofenacum on the acute cholecystitis, after intramuscular administration After the induction some groups received Dicolfenacum until the laparoscopic cholecystectomy, which was performed after 3 or 7 days. Hematoxylin and eosin stain were done from the gallbladder sections. At the reintervention moment there were noticed many gallbladder-epiploic, gallbladder-gastric, gallbladder-enteral and parietal-gallbladder adherences. Macroscopic, the intensity of the gallbladder and near gallbladder inflammatory process was strongly marked in the group which was operated later making more difficult the cholecystectomy. The histology emphasized the gallbladder's inflammation, colagenolysis and vascular alteration. The experimental induction of the acute acalculous cholecystitis is possible by injecting using laparoscopy into the gallbladder L-α-Phosphatidylcholine in concentration over 0.5 mmol/l, the marked inflammatory lesions being emphasized also macroscopic and microscopic, but much decreased in animals who received Diclofenacum. [ABSTRACT FROM AUTHOR]

The experimental study aimed to induce the acute alithiasic cholecystitis by application of clips and then sectioning the cystic duct and cystic artery in 10 pigs using laparoscopy, to verify if the ischaemia of the gallbladder and the oclusion of the cystic duct produce inflammation and so the acute cholecystitis. The laparoscopic cholecystectomy was performed after 3 and 7 days. Hematoxylin and eosin stain was done from the sections of the gallbladder. At the reintervention moment, after 3 days, the sections presented the consequences of an important ischaemia of the tissues and we noticed that there aren't any signs of an acute inflammation after the ligature and section of cystic duct and artery. After 7 days the restoration of the vascularization with the regeneration of the structures are signs of the overcome of the ischaemia period and the reperfusion of the tissues [ABSTRACT FROM AUTHOR]

Background: Complex incisional hernia is still a debatable topic, with increasing incidence and an increased local and systemic postoperative morbidity and mortality. The size of the defect is a risk factor for both difficult closure and 30-day readmission due to complications. The main option for closure such defect is a mesh augmented component separation technique. The goal was to evaluate 30-day wound events and general complications including 90 days mortality., Material and Methods: We present a retrospective study that includes patients from two different university hospitals who underwent open incisional hernia repair with anterior component or posterior component separation between January 2015 and December 2021. Only non-contaminated adult patients (over 18 years old) with postoperative primary or recurrent median abdominal wall defects larger than 6 cm and with complete fascial closure were included. Demographics (age, gender, Body Mass Index-BMI, American Society of Anesthesiologists Classification-ASA score), recurrence rank, and co-morbidities), operative details, patient outcomes complications were collected. A native abdomen/pelvis computerized tomography (CT) scan was performed preoperatively in all patients and the anatomy of the defect and volumetry (abdominal cavity volume, incisional hernia volume and peritoneal volume) were evaluated. One of the component separation technique was performed according to Carbonell's equation., Results: Two hundred and two patients (101 from each group) were included. The patients with posterior component separation were more comorbid and with larger defects. The procedure was longer with 80 min but overall length of hospital stay shorter (p < 0.001) for posterior component separation. Seroma, hematoma and skin necrosis were equally distributed for both group of patients and there was no direct relation to surgery (OR 0.887, 95% CI 0.370-2.125, p = 0.788; OR 1.50, 95% CI 0.677-3.33, p = 0.318 and OR 0.386, 95% CI 0.117-1.276, p = 0.119). Surgical Site Infection rate was increased for anterior component separation (p =0.004)., Conclusion: Complex incisional hernia repair is a challenge given by a large amount of wound complications. Choosing between anterior and posterior component separation is still a source of significant debate. We were not able to depict significant different rates of complications between the procedures and we couldn't find any specific factor related to complications., (© 2023. The Author(s), under exclusive licence to Springer-Verlag France SAS, part of Springer Nature.)

Introduction: This prospective study aims to illustrate the histopathological arterial changes in the popliteal artery in peripheral arterial disease of the lower limbs., Material and Method: A total of 60 popliteal artery segments taken from patients who had undergone lower limb amputation were examined between April and June 2023. The degree of arterial stenosis, medial calcinosis, and the vasa vasorum changes in the arterial adventitia were quantified. The presence of risk factors for atherosclerosis was also observed., Results: Atherosclerotic plaque was found in all of the examined segments. Medial calcinosis was observed in 40 (66.6%) of the arterial segments. A positive association between the degree of arterial stenosis and the vasa vasorum changes in the arterial adventitia was also found ( p = 0.025). The level of blood sugar and cholesterol were predictive factors for the severity of atherosclerosis., Conclusions: Atherosclerosis and medial calcinosis are significant in patients who underwent lower limb amputation. Medial calcinosis causes damage to the arterial wall and leads to a reduction in responsiveness to dilator stimuli.

Objectives: The broad spectrum of chronic venous disease encompasses varicose veins, edema, hyperpigmentation and venous ulcers. Radiofrequency thermal ablation is indicated for the treatment of superficial venous reflux of the lower limb. Our research is a comparative clinical study that aims to identify the most effective and safest therapeutic method in the management of chronic venous insufficiency of the lower limbs., Materials and Methods: Patients admitted to the Department of Surgery of the University of Medicine and Pharmacy in Cluj-Napoca, Romania, with the clinical diagnosis of varicose veins of the lower limbs, treated by thermal ablation with radiofrequency or by open surgical techniques during the year 2022, were included., Results: A percentage of 50.9% of the patients were treated by the radiofrequency thermal ablation procedure and 49.1% by surgical treatment. More than half of them were hospitalized for 2 days. The duration of hospitalization was significantly longer in patients who had postoperative complications ( p < 0.001). The chance of being treated by open surgical treatment for a small saphenous vein is 10.11 times higher than by radiofrequency thermal ablation., Conclusion: According to the applied tests, there is no statistical difference between the group treated by radiofrequency thermal ablation and the one surgically treated in terms of sex, age, origin, CEAP clinical stage at hospitalization, clinical diagnosis at hospitalization and affected lower limb.

Aim: The aim of the present study is to investigate the incidence of accidental parathyroidectomy and the connection between the type of surgery, or the resected piece sent for histopathological examination and the number of accidentally excised parathyroid glands., Material and Methods: Patients who had thyroid surgery between January 2005 and December 2014 and were admitted to a surgery clinic from Targu Mures, Romania, were enrolled in this study. For statistical analysis we used Chi-squared test, Student's t-test and ANOVA test, with a p value < 0.05 considered statistically significant., Results: A total of 3065 patients (315 males, 2750 females) were included in our study, with a mean age of 49.66±13.73. The frequence of incidental parathyroidectomy was 15.36%, most patients with IPT (88.95%) had only one parathyroid gland removed and we found a statistically significant association (p = 0.01) between the incidence of IPT and the type of surgery., Discussion: Iatrogenic injury of the parathyroid glands cause hypoparathyroidism which can be transient in majority and permanent in 1.5% of the patients. The most frequent cases with accidental removal of the parathyroid glands were total and subtotal thyroidectomies (79.6%), respectively reinterventions or completion thyroidectomies (10.62%)., Conclusions: Incidental parathyroidectomy is not uncommon following thyroid surgery, even in the hands of experienced surgeons and it is more often seen in female patients with polynodular goiter according to our study. Total thyroidectomies and reinterventions on the thyroid gland increase the risk of incidental parathyroidectomy., Key Words: Incidental parathyroidectomy, Hypoparathyroidism, Parathyroid glands.

Aim: Breast cancer is known as the most frequent cancer type among women. In several developing countries advanced stage cases present an increase trend, despite the global provisions of screening for early detection. The aim was to investigate patients with locally advanced breast cancers, in a developing country from eastern Europe., Material and Methods: A retrospective study was performed, including patients diagnosed with breast cancer who underwent surgical intervention, during 2007-2017. Besides demographic data, surgical techniques were investigated. Within histopathological data tumor size, type and grade were examined. We also investigated lymph node status and patient's hormonal parameters., Results: We examined 1008 patients diagnosed with benign and malignant mammary gland tumors over 11 years. After excluding benign tumors, inflammatory cancers, biopsies, recurrent breast cancers and initial stages, 125 patients remained eligible. Exulceration and hemorrhage were observed in 64 (51.2%) locally advanced cases. Resection of the pectoralis major muscle was realized in 12.8% due to tumoral infiltration., Discussion: Locally advanced breast cancer represents approximately 5% in developed countries. Within our results, this rate was 27.9%. This discrepancy is given by the regular national mammary screening programs within several developed countries., Conclusions: In the developing countries locally advanced breast cancer presents a continuous increase and hemorrhagic exulcerated types are not uncommon. Due to the poor health education and sometimes inadequate health care in eastern Europe, just a few patients have benefited of neoadjuvant therapy and preoperative mammography was performed in a small number of patients., Key Words: Brest Cancer, Locally Advanced.

Background: Neutrophil-to-lymphocyte ratio (NLR) has recently emerged as a useful predictor of cardiovascular risk and adverse outcomes. According to previous studies, an NLR >5 has the highest sensitivity and specificity for postoperative morbidity and mortality in cardiovascular disease. This study aims to evaluate the NLR in cases of infrarenal unruptured abdominal aortic aneurysm (uAAA) and ruptured abdominal aortic aneurysm (rAAA) and to assess the role of NLR as a prognostic marker of 30-day mortality in patients with uAAA and rAAA who underwent surgical repair., Methods: This retrospective cohort study examined 255 consecutive patients with intact or ruptured infrarenal AAA who underwent elective or urgent open repair surgery within our clinic in a 10-year period. Differences in prevalence were assessed using chi-squared calculations and values greater than 5 and a P-value less than 0.05 were considered significant. The averages were compared using the ANOVA parameter test when the Bartlett P-value was greater than 0.05., Results: The average NLR appeared to be significantly higher in the group of patients with rAAA (9.3 vs. 3.39, respectively P < 0001). Furthermore, NLR > 5 occurred in 77.6% of patients with rAAA but only 32.5% in patients with uAAA (odds ratio 5.085; 95% confidence interval [CI]: 3.0025-8.6145; P < 0000.1). In terms of the postoperative prognosis in patients with uAAA, mortality after 30 days postoperatively was considerably higher at 16.6% in patients with NLR >5 compared with 6% for patients with NLR < 5 (RR: 2.77; 95% CI: 1.020-7.55; P < 0.045). In the case of rAAA, mortality after 30 days was higher in patients with NLR >5 (61.44%) than those with NLR < 5 (45.83%). There was no relationship between NLR and length of hospital stay or between NLR and the maximum diameter of the AAA. There was also no difference in the NLR between genders or age groups., Conclusions: The main findings of this study were the poor outcomes in terms of 30-day mortality for the patients presenting NLR values greater than 5 undergoing open surgical repair in both categories: infrarenal uAAA and rAAA. We also show that NLR is significantly higher among patients with rAAA and that an NLR >5 indicates a 5 times greater possibility of AAA being ruptured. We can use this easily determinable, broadly available, and inexpensive marker to identify high-risk patients, individually, or integrated into a risk-stratification system for patients diagnosed with AAA. This would help in the therapeutic management of AAA, including the avoidance of open surgery when there are prohibitive risks, instead opting for an endovascular approach., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Background: Inguinal hernia repair is one of the most common surgical procedures in man. Immediate postoperative pain is an important issue that can delay hospital discharge. Besides, the presence of chronic pain after herniorrhaphy, which can affect up to 50% of patients, is a growing concern. However information regarding the precise etiological factors of this chronic postoperative pain is lacking. One factor thought to contribute to post herniorrhaphy chronic pain is the surgical procedure for inguinal hernia repair used by the surgeon., Materials and Method: The study was conducted over a period of 5 years and included 1000 consecutive patients operated with inguinal hernia. Each patients completed a questionnaire about the presence or absence of pain or sensory disorders. After completed only 365 of patients remains in the study. From this patients, a total of 38 had different intensity of pain. From those, 13% were operated through an tissular procedure, whereas the laparoscopic procedure was responsible only for 7% of the patients with chronic postoperative pain. Most of the patients had mild or moderate pain and only one patient experienced severe pain. According to the type of procedure performed, in 25 patients were used the tissular procedure and only 12 patients with laparoscopic hernia repair had chronic pain., Conclusion: The etiology of chronic groin pain post hernia repair is related in part to nerve injury. This is supported by the high frequency of sensory symptoms and numbness in these patients. However other factors including the role of tissue injury and inflammatory postoperative changes need to be considered., Key Words: Chronic postoperative pain, Inguinal hernia repair, Open surgery, Laparoscopic surgery.

Infections after vascular reconstructions are very rare; however, when they occur, they are associated with a high risk of morbidity. In order to obtain the best results possible, the treatment needs to be initiated as early as possible, from the very first signs of infection, and it needs to be carried out in centers specializing in vascular surgery. The aim of the present study was to assess the incidence of infections in a single university center.This retrospective analysis over a 2-year period is based on the medical reports of hospitalized patients who were diagnosed with infection following revascularization.From 2013 to 2014, a number of 151 open reconstructive surgical procedures were performed. 15 patients suffered from infection (10%) of the vascular reconstruction. Of these patients, 40% have had an aorto-bifemoral bypass, 53%-a femoro-popliteal bypass, and 7% (n = 1)-an axillo-femoral bypass. According to the Samson classification, the patients were categorized as follows: group 2: 6 cases, group 3: 2 cases, group 4: 4 cases, and group 5: 3 cases. The most frequent bacteria found were methicillin-resistant Staphylococcus aureus (MRSA) (n = 6, 40%), followed by S aureus (n = 5, 33%). The treatment options were: application of antibiotics alone without any invasive treatment in 3 patients, local irrigation and debridement in 6 patients, complete explantation of the prosthesis with a new extra-anatomic bypass in 6 cases, and partial excision of the prosthesis, which was replaced and covered with muscle flap, in 3 cases. The amputation rate in our study was 18%, which corresponds to the rates published in the literature.The treatment of infections in vascular surgery needs to be complex and adapted to each individual patient, because infections being in a permanent dynamic state. The treatment needs to be performed in specialized centers that have large experience in vascular surgery, in order for the patient to have the best chances of survival and protection from amputation.

Background: Acute cholecystitis is still frequent in emergency surgical departments. As surgical technique, nowadays laparoscopy is widely used and with low complications and with low postoperative morbidity., Material and Methods: We perform an analytical study about the safety of laparoscopic surgery in patients with acute cholecystitis in a single Surgical Department with an experience of over 20 years in laparoscopic surgery. We included 193 patient admitted in our department during 2014 and 2015., Results: Of the 193 patients, 43% were diagnosed with acute lithiasic cholecystitis (ALC) whereas 56% had chronic lithiasic cholecystitis (CLC). We assessed the comorbidities of the patient via Pearson's Chi-Square test and we found out that there is a significant relationship between acute cholecystitis and high blood tension, obesity and diabetes. Surgical techniques performed were in 95% of cases laparoscopic cholecystectomy and only in 5% we performed open surgery., Discussions: Experienced surgeons have a lower conversion rate as compared to less experienced surgeons. For this reason, postoperative assessment criteria have been proposed, with a view to identify the risk of conversion CONCLUSION: In our study laparoscopic surgery for acute cholecystitis is a safe procedure with low intraoperative complication rate and with a reduced hospital stay., Key Words: Acute cholecystitis, Intraoperative adhesion, Intraoperative bleeding, Laparoscopic cholecystectomy.

Background: Data from the literature regarding the prognostic role of DNA mismatch repair system (MMR) in colorectal cancer are still controversial., Aim: The aim of the study was to identify the prognostic role of different phenotypic, clinical and pathological characteristics in microsatellite unstable vs. microsatellite stable colorectal cancer in terms of survival and disease free interval., Methods: We conducted a retrospective study that included a total of 103 patients who underwent curative surgery for colorectal cancer. Immunohistochemistry testing revealed MLH1, MLH2, MLH6, PMS2 genes and mutations of the BRAF gene. We identified three groups of patients: patients with colorectal tumors with MSI produced by hypermethylation, (MLH1/BRAF+) group, patients with microsatellite instable tumours produced by genetic mutations MSI groupb(MLH1, MLH2, MLH6, PMS2) and patients with microsatellite stable tumours (MSS)., Results: The study shows that: MSI tumours (MLH1/BRAF+) group occur more frequently in women (p=0.05), on the right side of the colon (p=0.001). The 5-year survival rate was higher in patients with MSI tumours (MLH1/BRAF+) group than in those with microsatellite stable tumours, the differences were not statistically significant ; relapse rate was higher in patients with MSI tumors than in those with MSI tumours (MLH1/BRAF+) group (p=0.03) or with MSS tumors (p=0.004)., Conclusions: The identification of microsatellite unstable colorectal tumours is an important molecular marker with role in recognition subgroups of patients with different phenotypic characteristics, survival and relapse rates., Key Words: Colorectal cancer, Mismatch repair genes, Prognostic role.

Surgery remains the gold standard for the treatment of patients with echinococcosis, despite significant economic costs, advances in medical treatment and interventional radiology; in the past decades there has been a tendency toward laparoscopic surgery. We present a 66-year-old patient, from a rural area, who was admitted to our service complaining of spontaneous and palpatory pains in the right hypocondrium, headaches and dizziness. Abdominal CT scan highlighted 2 round calcified tumors, one of 7.2 cm diameter (VIII(th) segment) with liquid densities, and the other one localized higher, with a diameter of 2.3 cm (IV(th) segment). Under general anesthesia, after the neutralization of the content of the cyst with hypertonic saline irrigation, we performed laparoscopic partial pericystectomy of the VIII(th) segment liver cyst and total laparoscopic cystectomy of the IV(th) segment liver cyst. Postoperative evolution was favorable without biliary fistula formation, postoperative infections or cystic cavities abscesses. Laparoscopic surgery seems to be effective and safe for uncomplicated cysts in accessible segments of the liver, combined with adjuvant albendazole therapy to reduce complications and postoperative morbidity, but the procedure has its own disadvantages such as a limited area of surgical manipulation.

Aim: This prospective study was undertaken to determine how peripheral atherosclerotic disease influences the flow in the deep veins of the leg., Material and Method: Thirty one subjects with peripheral atherosclerotic disease and 23 age matched control subjects were studied. The popliteal vein flow velocity was measured at rest and during reactive hyperemia by means of color duplex ultrasound scanning. Patient age, ankle-brachial index (ABI) and the presence of risk factors for venous thrombosis were also recorded., Results: There was a negative correlation between the ankle-brachial index and venous flow velocity among subjects with peripheral arterial disease (p=0.001). There was a negative correlation between dyslipidemia and resting venous flow velocity (p=0.049). During reactive hyperemia, venous flow velocity increased less in subjects with peripheral arterial disease than it did in control subjects (p=0.007). The subjects with dyslipidemia showed no changes in venous flow velocity in reactive hyperemia measurements (p=0.908)., Conclusion: Increasing the venous flow velocity in peripheral arterial disease, may confer some protection against the deep venous thrombosis.

Severe acute pancreatitis is a critical illness as the organism that produces a significant mortality despite diagnostic and therapeutic acquisitions. While new mechanisms have been identified for production and were crystallized management principles, a number of controversies remain awaiting resolution in the near future. Aim is to establish, based on their experience and literature data, place the current means of diagnosis and treatment in close correlation with the pathophysiological events of acute pancreatitis.

Mesenteric venous thrombosis (MVT), an unusual location of deep venous thrombosis, occurs especially on a predisposing terrain. Recently, hyperhomocysteinemia has been shown to be associated with venous thrombosis, often recurrent and located in an uncommon site. Hyperhomocysteinemia is mainly due to genetic causes (mutations 677C>T and 1298A>C of methylenetetrahydrofolate reductase) and vitamins B deficiencies. MVT may present as acute, subacute or chronic form. The clinical supposition of mesenteric thrombosis is based on the discrepancy between the abdominal pain and the physical examination. The nonspecific character of the pain, mimicking peptic ulceration in some cases, and the possibility of an initial normal clinical examination may delay the diagnosis. The occurrence of the fever, rebound tenderness and guarding suggests progression to bowel infarction. MVT leads to peritonitis in 1/3 to 2/3 of cases. Laboratory blood tests are not helpful in confirming the diagnosis of venous thrombosis. Leukocytosis and metabolic acidosis are considered to be the most specific laboratory findings in patients with mesenteric ischemia. Abdominal computed tomography is the test of choice for the diagnosis. However, most of the cases are diagnosed during laparotomy or autopsy. Anticoagulant therapy administrated early increases the survival rate. Surgery is indicated in cases with bowel infarction or peritonitis.

The case of a 30-year-old man with bowel infarction due to mesenteric venous thrombosis and multiple risk factors, including mild hyperhomocysteinemia due to methylenetetrahydrofolate reductase C677T polymorphism and recent abdominal surgery, is reported. His clinical manifestation consisted of persistent abdominal pain; complementary examinations showed nonspecific findings such as leukocytosis and dilated loops of the bowel. The diagnosis of mesenteric venous thrombosis with bowel infarction was made during laparotomy and confirmed by anatomopathologic examination. He underwent segmental resection associated with lifelong anticoagulant therapy and vitamin B supplementation with a favorable course.

The occlusive aorto-iliac disease is the consequence of a diffuse atherosclerotic process aggravated by risk factors and existing co-morbidity. The treatment aims at correcting the risk factors, balancing and compensating the associated diseases, the surgical re-vascularization of the lower limbs (by pass, particular techniques for the aortic aneurysms, necessity interventions). Aortic and peripheral angiographic exploration is required for the establishment of surgical strategy. The lot studied includes 77 patients hospitalized in the period between 2000-2006 in the Surgery Clinic no. II, Cluj-Napoca. 33 cases had an obstruction of a single iliac artery. The bilateral affection has been present in 27 cases, while that of the terminal trunk of the aorta and of its bifurcation has been present in 17 cases. The most frequent intervention has been the aorto-bifemoral prosthesis, the prosthesis used being: Terom, Dacron and PTFE. The thrombendarterectomy was used as an independent method or associated with the by-pass. At the same time, the aortoiliac interventions were associated with aorto-inguinal bypass, crossover as well as disarticulations and amputations of toes. 26% of the 77 patients, had early post-surgery complications (hemorrhage, infection, the thrombosis of the graft). The re-vascularization failed in 3.8% of cases, a major amputation of the limb being necessary. 11 deaths (14.3%) were recorded which occurred as a result of systemic complications.

The interest manifested for the conjunctive tissue pathology leaded to the study of the structural disorder that appears in the varicose veins walls. The study is a prospective one initiated in March 2007 made on 11 patients with varicose disease hospitalized at Cluj-Napoca within Surgery Clinic no. II. The purpose of this study is to point out the histopathological modifications in the varicose venous wall (great saphenous vein cross, communicating veins, perforating veins), as well as the correlation of histopathological results with the evolutive stage of chronic vein insufficiency (CEAP classification) and with the clinical score at these patients. The histopathological (HP) results for 2 of the patients revealed hypertrophy of the media, intimal hyperplasia (stage II) corresponding to a CEAP 3. Six patients were integrated in HP stage III due to the partial intimal fibrosis corresponding to a CEAP 6 for one case, CEAP 4 one case, CEAP 3 four cases. One patient had HP stage I with CEAP 3 and two patients had HP stage IV corresponding to CEAP 5, respectively CEAP 6.

The heterotopic transplantation of isolated colon segment was achieved in the cervical region, for to study the behaviour of the colon in this new situation. The study included 20 dogs from which there were taken segments of 10 cm length from, the left colon. Then was prepared the common carotid artery, superficial jugular vein and their branches. In 5 dogs the cervical esophagus was resected. Then was performed the transplant using micro-surgical techniques. In 15 dogs the colon was exteriorized at the skin and in 5 dogs the colon was placed between the ends of the esophagus. In 76% of cases the postoperative evolution was good. From the viable colon transplants were taken fragments for histologic examinations after 1, 2 respectively 4 weeks postoperative. The results obtained, recommend the colic transplant as a possible graft for repairing the cervical esophagus defects.