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41 results on '"Anaya-Velázquez, Fernando"'

1. Avirulent UG10 Entamoeba histolytica mutant derived from HM-1:IMSS strain shows limited genome variability and aberrant 5-methyl cytosine genomic distribution

Author

Vargas-Maya, Naurú Idalia, Maunakea, Alika K., Ramírez-Montiel, Fátima Berenice, Sultana, Razvan, Peres, Rafael, Macías-Cervantes, Quetzalli Xiadany, Medina-Nieto, Ana Laura, Rangel-Serrano, Ángeles, Martínez-Álvarez, José A., Páramo-Pérez, Itzel, Anaya-Velázquez, Fernando, Padilla-Vaca, Felipe, and Franco, Bernardo

Published
2024
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4. Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA

Author

Padilla-Vaca, Felipe, primary, de la Mora, Javier, additional, García-Contreras, Rodolfo, additional, Ramírez-Prado, Jorge Humberto, additional, Vicente-Gómez, Marcos, additional, Vargas-Gasca, Francisco, additional, Anaya-Velázquez, Fernando, additional, Páramo-Pérez, Itzel, additional, Rangel-Serrano, Ángeles, additional, Cuéllar-Mata, Patricia, additional, Vargas-Maya, Naurú Idalia, additional, and Franco, Bernardo, additional

Published
2023
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8. Attenuation of In Vitro and In Vivo Virulence Is Associated with Repression of Gene Expression of AIG1 Gene in Entamoeba histolytica

Author

Lozano-Mendoza, Janeth, primary, Ramírez-Montiel, Fátima, additional, Rangel-Serrano, Ángeles, additional, Páramo-Pérez, Itzel, additional, Mendoza-Macías, Claudia Leticia, additional, Saavedra-Salazar, Faridi, additional, Franco, Bernardo, additional, Vargas-Maya, Naurú, additional, Jeelani, Ghulam, additional, Saito-Nakano, Yumiko, additional, Anaya-Velázquez, Fernando, additional, Nozaki, Tomoyoshi, additional, and Padilla-Vaca, Felipe, additional

Published
2023
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9. Escherichia coli transcription factors of unknown function: sequence features and possible evolutionary relationships

Author

Duarte-Velázquez, Isabel, primary, de la Mora, Javier, additional, Ramírez-Prado, Jorge Humberto, additional, Aguillón-Bárcenas, Alondra, additional, Tornero-Gutiérrez, Fátima, additional, Cordero-Loreto, Eugenia, additional, Anaya-Velázquez, Fernando, additional, Páramo-Pérez, Itzel, additional, Rangel-Serrano, Ángeles, additional, Muñoz-Carranza, Sergio Rodrigo, additional, Romero-González, Oscar Eduardo, additional, Cardoso-Reyes, Luis Rafael, additional, Rodríguez-Ojeda, Ricardo Alberto, additional, Mora-Montes, Héctor Manuel, additional, Vargas-Maya, Naurú Idalia, additional, Padilla-Vaca, Felipe, additional, and Franco, Bernardo, additional

Published
2022
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10. Intellectual Curve Scene Text Detection from Natural Images Using MSER Descriptor Based Region Segmentation Approach

Author

C. Teixeira Samuel, Kalita Jahnabi, Wang Huayuan, Qin Weiwei, Shi Yingying, Xia Mingrong, Kumar Deepak, Yang Miaomiao, Páramo-Pérez Itzel, Elizabeth Reyes-Martínez Juana, Chandra Remya, S. Lopes Daiana, SIngh Ramandeep, Padilla-Vaca Felipe, Tamayo-Nuñez Jessica, Anaya-Velázquez Fernando, de Melo Rodrigues Veridiana, Chetia Dipak, Idalia Vargas-Maya Naurú, Li Gai, Franco Bernardo, Zhang Jiewen, Sun Ruihua, Liliana España-Sánchez Beatríz, Zhang Haohan, Zhao Jing, Abdizadeh Tooba, Vijayan Dileep, N.C. Gimenes Sarah, Sun Yajing, Banerjee Rintu, de la Mora Javier, Abdizadeh Rahman, Jiménez-Charris Eliécer, Rangel-Serrano Ángeles, Ma Limin, Solano-Redondo Luis, Rudrapal Mithun, Hadizadeh Farzin, and Montealegre-Sánchez Leonel

Subjects
Control and Optimization, Computer Networks and Communications, Computer science, business.industry, Natural (music), Segmentation, Pattern recognition, Text detection, Artificial intelligence, Electrical and Electronic Engineering, business, Computer Science Applications
Abstract

Background: A novel method to detect the text region from the natural image using the discriminative deep feature of text regions is presented with deep learning concept in this manuscript. Objective: Curve Text Detection (CTD) from the natural image is generally based on two different tasks: learning of text data and text region detection. In the learning of text data, the goal is to train the system with a sample of letters and natural images, while, in the text region detection, the aim is to confirm whether the detected regions are text region or not. The emphasis of this research is on the development of deep learning algorithm. Methods: A novel approach has been proposed to detect the text region from natural images which simultaneously tackles three combined challenges: 1) pre-processing of the image without losing text region; 2) appropriate segmentation of text region using their strokes, and 3) training of data. In pre-processing, image enhancement and binarization are done then morphological operations are defined with the Maximally Stable Extremal Region (MSER) based segmentation technique which operates on the basis of stroke region of text and then finds out the (Speed Up Robust Feature) SURF key point from those regions. Results: Based on the SURF feature, text region is detected from the images using a trained structure of Artificial Neural Network (ANN) which is based on deep learning mechanism. Conclusion: CTW-1500 dataset is used to simulate the proposed work and the parameters like Precision, Recall, F-Measure (H-mean), Execution time, Accuracy and Error Rate are computed and are compared with the existing work to depict the effectiveness of the work.

Published
2021
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21. aeBlue Chromoprotein Color is Temperature Dependent

Author

Tamayo-Nuñez, Jessica, primary, de la Mora, Javier, additional, Padilla-Vaca, Felipe, additional, Vargas-Maya, Naurú Idalia, additional, Rangel-Serrano, Ángeles, additional, Anaya-Velázquez, Fernando, additional, Páramo-Pérez, Itzel, additional, Reyes-Martínez, Juana Elizabeth, additional, España-Sánchez, Beatríz Liliana, additional, and Franco, Bernardo, additional

Published
2019
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22. Plasma membrane damage repair is mediated by an acid sphingomyelinase in Entamoeba histolytica

Author

Ramírez-Montiel, Fátima, primary, Mendoza-Macías, Claudia, additional, Andrade-Guillén, Sairy, additional, Rangel-Serrano, Ángeles, additional, Páramo-Pérez, Itzel, additional, Rivera-Cuéllar, Paris E., additional, España-Sánchez, B. Liliana, additional, Luna-Bárcenas, Gabriel, additional, Anaya-Velázquez, Fernando, additional, Franco, Bernardo, additional, and Padilla-Vaca, Felipe, additional

Published
2019
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23. Development of an oxidative stress sensor in live bacteria using the optimized HyPer2 protein

Author

Franco, B., primary, Padilla-Vaca, Felipe, additional, Vargas-Maya, Naurú Idalia, additional, Herrera-Gutiérrez, Luz Janeth, additional, Mora-Garduño, Josué Daniel, additional, Tafoya-Ramírez, Margarita Daniela, additional, Páramo-Pérez, Itzel, additional, Anaya-Velázquez, Fernando, additional, and Mendoza-Macías, Claudia Leticia, additional

Published
2018
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25. aeBlue Chromoprotein Color is Temperature Dependent

Author

Tamayo-Nuñez, Jessica, de la Mora, Javier, Padilla-Vaca, Felipe, Vargas-Maya, Naurú I., Rangel-Serrano, Ángeles, Anaya-Velázquez, Fernando, Páramo-Pérez, Itzel, Reyes-Martínez, Juana Elizabeth, España-Sánchez, Beatríz Liliana, and Franco, Bernardo

Abstract

Background: Marine sessile organisms display a color palette that is the result of the expression of fluorescent and non-fluorescent proteins. Fluorescent proteins have uncovered transcriptional regulation, subcellular localization of proteins, and the fate of cells during development. Chromoproteins have received less attention until recent years as bioreporters. Here, we studied the properties of aeBlue, a a 25.91 kDa protein from the anemone Actinia equina. Objective: To assess the properties of aeBlue chromoprotein under different physicochemical conditions. Methods: In this article, during the purification of aeBlue we uncovered that it suffered a color shift when frozen. We studied the color shift by different temperature incubation and physicochemical conditions and light spectroscopy. To assess the possible structural changes in the protein, circular dichroism analysis, size exclusion chromatography and native PAGE was performed. Results: We uncover that aeBlue chromoprotein, when expressed from a synthetic construct in Escherichia coli, showed a temperature dependent color shift. Protein purified at 4 °C by metal affinity chromatography exhibited a pinkish color and shifts back at higher temperatures to its intense blue color. Circular dichroism analysis revealed that the structure in the pink form of the protein has reduced secondary structure at 4 °C, but at 35 °C and higher, the structure shifts to a native conformation and Far UV- vis CD spectra revealed the shift in an aromatic residue of the chromophore. Also, the chromophore retains its properties in a wide range of conditions (pH, denaturants, reducing and oxidants agents). Quaternary structure is also maintained as a tetrameric conformation as shown by native gel and size exclusion chromatography. Conclusion: Our results suggest that the chromophore position in aeBlue is shifted from its native position rendering the pink color and the process to return it to its native blue conformation is temperature dependent.

Published
2020
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26. Synthetic biology: Novel approaches for microbiology

Author

Padilla-Vaca, Felipe, Anaya-Velázquez, Fernando, Franco, Bernardo, Padilla-Vaca, Felipe, Anaya-Velázquez, Fernando, and Franco, Bernardo

Abstract

In the past twenty years, molecular genetics has created powerful tools for genetic manipulation of living organisms. Whole genome sequencing has provided necessary information to assess knowledge on gene function and protein networks. In addition, new tools permit to modify organisms to perform desired tasks. Gene function analysis is speed up by novel approaches that couple both high throughput data generation and mining. Synthetic biology is an emerging field that uses tools for generating novel gene networks, whole genome synthesis and engineering. New applications in biotechnological, pharmaceutical and biomedical research are envisioned for synthetic biology. In recent years these new strategies have opened up the possibilities to study gene and genome editing, creation of novel tools for functional studies in virus, parasites and pathogenic bacteria. There is also the possibility to re-design organisms to generate vaccine subunits or produce new pharmaceuticals to combat multi-drug resistant pathogens. In this review we provide our opinion on the applicability of synthetic biology strategies for functional studies of pathogenic organisms and some applications such as genome editing and gene network studies to further comprehend virulence factors and determinants in pathogenic organisms. We also discuss what we consider important ethical issues for this field of molecular biology, especially for potential misuse of the new technologies. [Int Microbiol 2015; 18(2):71-84]Keywords: synthetic biology · genetic engineering · genomics · pathogenesis · bioethics · artificial cells · astrobiology

Published
2015

28. Nanocomposites based on Polypropylene and Copper Nanoparticles: Preparation, Surface Activation by Plasma and Antibacterial Activity

Author

España-Sánchez, B. Liliana, primary, Rodríguez-González, J. Alberto, additional, González-Morones, Pablo, additional, Neira-Velázquez, M. Guadalupe, additional, Franco-Bárcenas, Bernardo, additional, Anaya-Velázquez, Fernando, additional, Mendoza-Macías, Claudia Leticia, additional, Ávila-Orta, Carlos A., additional, and Padilla-Vaca, Felipe, additional

Published
2014
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30. Flora Normal, Probióticos y Salud Humana

Author

Gamiño-Arroyo, Ana Estela, primary, Barrios-Ceballos, Minerva Paola, primary, Cárdenas de la Peña, Lydia Patricia, primary, Anaya-Velázquez, Fernando, primary, and Padilla-Vaca, Felipe, primary

Published
2005
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34. Nanocompuestos de polipropileno con nanopartículas de cobre: preparación, activación superficial por plasma y actividad antibacteriana.

Author

España-Sánchez, B. Liliana, Rodríguez-González, J. Alberto, González-Morones, Pablo, Neira-Velázquez, M. Guadalupe, Franco-Bárcenas, Bernardo, Anaya-Velázquez, Fernando, Mendoza-Macías, Claudia Leticia, Ávila-Orta, Carlos A., and Padilla-Vaca, Felipe

Subjects
NANOCOMPOSITE materials, POLYPROPYLENE, POLYPROPYLENE manufacturing, ANTIBACTERIAL agents, COPPER, ARGON plasmas, SCANNING electron microscopy, ATOMIC force microscopy
Abstract

Copyright of Acta Universitaria is the property of Universidad de Guanajuato/Acta Universitaria and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2014
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36. IMPLICACIONES BIOÉTICAS DE LOS ORGANISMOS TRANSGÉNICOS.

Author

Anaya-Velázquez, Fernando

Subjects
*TRANSGENIC organisms, *GENETIC engineering, *GENETICALLY modified foods, *FOOD industry, *BIOETHICS, *ETHICS
Abstract

Through the development of biological science, humankind was able to obtain new varieties of animals and plants in a process beginning with the wild species. When genetic engineering techniques were applied to these organisms, several dozens of transgenic organisms were obtained including plants and animals with specific inheritable changes, which are now transgenic foods. From the bioethical analysis of genetically modified organisms the article argues in favor of the right of the consumer to be informed in terms he or she can understand on the nature of genetic changes induced in foods, as well as in favor of the right of consumers to choose between standard or modified food. Finally, the article also argues in favor of the moral and scientific duties of scientists and food industries to demonstrate that genetically modified foods are not dangerous to human beings or noxious to the environment or biodiversity. [ABSTRACT FROM AUTHOR]

Published
2006

40. Synthetic biology: Novel approaches for microbiology.

Author

Padilla-Vaca F, Anaya-Velázquez F, and Franco B

Subjects
Bacteria metabolism, Humans, Microbiology, Bacteria genetics, Synthetic Biology methods
Abstract

In the past twenty years, molecular genetics has created powerful tools for genetic manipulation of living organisms. Whole genome sequencing has provided necessary information to assess knowledge on gene function and protein networks. In addition, new tools permit to modify organisms to perform desired tasks. Gene function analysis is speed up by novel approaches that couple both high throughput data generation and mining. Synthetic biology is an emerging field that uses tools for generating novel gene networks, whole genome synthesis and engineering. New applications in biotechnological, pharmaceutical and biomedical research are envisioned for synthetic biology. In recent years these new strategies have opened up the possibilities to study gene and genome editing, creation of novel tools for functional studies in virus, parasites and pathogenic bacteria. There is also the possibility to re-design organisms to generate vaccine subunits or produce new pharmaceuticals to combat multi-drug resistant pathogens. In this review we provide our opinion on the applicability of synthetic biology strategies for functional studies of pathogenic organisms and some applications such as genome editing and gene network studies to further comprehend virulence factors and determinants in pathogenic organisms. We also discuss what we consider important ethical issues for this field of molecular biology, especially for potential misuse of the new technologies., (Copyright© by the Spanish Society for Microbiology and Institute for Catalan Studies.)

Published
2015
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41. Bioethics, bioweapons and the microbiologist.

Author

Anaya-Velázquez F

Subjects
Bioterrorism ethics, Humans, Warfare, Bioethics, Biological Warfare ethics, Microbiology ethics
Abstract

The analysis of behavior of man in the field of biology is carried out through bioethics, considered the science of the survival. In the microbiology, there are numerous discoveries related with pathogenic microorganisms, including those that can be used as weapons in a biological war or in an attack considered bioterrorism. The scientist involved in microbiology can participate with his knowledge in the development and improvement of bioweapons, however from the point of view of bioethics it is not acceptable that he works in an investigation related with these topics, because the defense research can evolve in offensive one. The war is an antisurvival activity, therefore it is not acceptable. In the same way, the biological weapons composed with virus, fungi or alive bacteria, or with toxins from them, neither they are morally accepted. After the terrorist attacks with anthrax in the United States in 2001, the world scientific community in the field of microbiology should show against the use of the microorganisms like bioweapons, at the time of promoting the idea that the responsible use for the microorganisms is a moral imperative for all microbiologists around the world, since the biological weapons are a threat for the human life.

Published
2002

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