41 results on '"Anaya-López, José Luis"'
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2. Diagnosis of Plant Viruses Using NGS to Support Virus Management and Tomato Breeding
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González-Pérez, Enrique, primary, Chiquito-Almanza, Elizabeth, additional, Villalobos-Reyes, Salvador, additional, Canul-Kú, Jaime, additional, and Anaya-López, José Luis, additional
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- 2024
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3. A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana
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Martínez-Marrero, Nadia, Avalos-Calleros, Jesús Aarón, Chiquito-Almanza, Elizabeth, Acosta-Gallegos, Jorge Alberto, Ambriz-Granados, Salvador, Anaya-López, José Luis, and Argüello-Astorga, Gerardo Rafael
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- 2020
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4. Diagnosis and Characterization of Plant Viruses Using HTS to Support Virus Management and Tomato Breeding.
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González-Pérez, Enrique, Chiquito-Almanza, Elizabeth, Villalobos-Reyes, Salvador, Canul-Ku, Jaime, and Anaya-López, José Luis
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TOMATO breeding ,PLANT viruses ,TOBACCO mosaic virus ,TOMATO diseases & pests ,GENETIC variation ,MIXED infections ,TOMATOES - Abstract
Viral diseases pose a significant threat to tomato crops (Solanum lycopersicum L.), one of the world's most economically important vegetable crops. The limited genetic diversity of cultivated tomatoes contributes to their high susceptibility to viral infections. To address this challenge, tomato breeding programs must harness the genetic resources found in native populations and wild relatives. Breeding efforts may aim to develop broad-spectrum resistance against the virome. To identify the viruses naturally infecting 19 advanced lines, derived from native tomatoes, high-throughput sequencing (HTS) of small RNAs and confirmation with PCR and RT-PCR were used. Single and mixed infections with tomato mosaic virus (ToMV), tomato golden mosaic virus (ToGMoV), and pepper huasteco yellow vein virus (PHYVV) were detected. The complete consensus genomes of three variants of Mexican ToMV isolates were reconstructed, potentially forming a new ToMV clade with a distinct 3' UTR. The absence of reported mutations associated with resistance-breaking to ToMV suggests that the Tm-1, Tm-2, and Tm-2
2 genes could theoretically be used to confer resistance. However, the high mutation rates and a 63 nucleotide insertion in the 3' UTR, as well as amino acid mutations in the ORFs encoding 126 KDa, 183 KDa, and MP of Mexican ToMV isolates, suggest that it is necessary to evaluate the capacity of these variants to overcome Tm-1, Tm-2, and Tm-22 resistance genes. This evaluation, along with the characterization of advanced lines using molecular markers linked to these resistant genes, will be addressed in future studies as part of the breeding strategy. This study emphasizes the importance of using HTS for accurate identification and characterization of plant viruses that naturally infect tomato germplasm based on the consensus genome sequences. This study provides crucial insights to select appropriate disease management strategies and resistance genes and guide breeding efforts toward the development of virus-resistant tomato varieties. [ABSTRACT FROM AUTHOR]- Published
- 2024
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5. Genetic structure of Jatropha curcas L. in Mexico and probable centre of origin
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Pecina-Quintero, Victor, Anaya-López, José Luis, Zamarripa-Colmenero, Alfredo, Núñez-Colín, Carlos Alberto, Montes-García, Noé, Solís-Bonilla, José Luis, and Jiménez-Becerril, María Fernanda
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- 2014
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6. Assessing the genetic diversity of castor bean from Chiapas, México using SSR and AFLP markers
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Pecina-Quintero, Víctor, Anaya-López, José Luis, Núñez-Colín, Carlos Alberto, Zamarripa-Colmenero, Alfredo, Montes-García, Noé, Solís-Bonilla, José Luis, and Aguilar-Rangel, María Rocío
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- 2013
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7. Rubí: nueva variedad mejorada de frijol negro para las áreas tropicales de Veracruz y Chiapas
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Ibarra Pérez, Francisco Javier, Tosquy Valle, Óscar Hugo, Rodríguez Rodríguez, José Raúl, Villar Sánchez, Bernardo, López Salinas, Ernesto, Anaya López, José Luis, Ibarra Pérez, Francisco Javier, Tosquy Valle, Óscar Hugo, Rodríguez Rodríguez, José Raúl, Villar Sánchez, Bernardo, López Salinas, Ernesto, and Anaya López, José Luis
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In Veracruz and Chiapas, Mexico, bean cultivation is mainly affected by the incidence of viral diseases, the occurrence of terminal drought in residual moisture sowingsand low-fertility acidic soils.To help solve this problem, the bean variety‘Rubí’ was developed.In 2015, the Jamapa Plus/XRAV-187-3-1-8 line, which gave rise to the Rubívariety, along with 49 other lines and 20 black bean varietieswere screened by artificial inoculation and the use of molecular markers, to determine their resistance tobeancommon mosaic virus (BCMV), bean common mosaic necrosis virus (BCMNV) and bean golden yellow mosaic virus (BGYMV),in said study, Rubí showed resistance to BCMV and low incidence of BGYMV.During 2015 and 2016, Rubíwas evaluated together with two regional controls in an adaptation nursery in six environments of Veracruz and Chiapas, to determine its productive response, under rainfed, residual moisture, irrigation and terminal droughtconditions.In four of the six environments, Rubí was superior in yield to the two regional controls, with an overall average of 10.3 and 18.1% higher than that obtained for the Negro Comapa and Negro Grijalva varieties, respectively. During 2016 and 2017, Rubíwas evaluated in a uniform yield trial in 10 environments in Veracruz and Chiapas. The new variety obtained a significantly outstanding average yield and showed better adaptation than Negro Comapa and Negro Grijalva.In 2019, the National Seed Inspection and Certification Service (SNICS) granted the definitive registration in the National Catalogue of Plant Varieties, with number FRI-099-06119, foritscommercial use, En Veracruz y Chiapas, México, el cultivo de frijol es afectadoporla incidencia de enfermedadesvirales, la ocurrencia de sequía terminal en las siembras de humedad residual y lossuelos ácidos de baja fertilidad. Para contribuir a solucionaresta problemática sedesarrolló la variedad de frijol ‘Rubí’.En 2015, la línea Jamapa Plus/XRAV-187-3-1-8,que dio origen a la variedad Rubí, junto con otras 49 líneas y 20 variedades de frijol negro, se tamizaron mediante inoculación artificial y el uso de marcadores moleculares, para determinar su resistencia al virus del mosaico común del frijol (BCMV), virus del mosaico necrótico común (BCMNV) y virus del mosaico amarillo dorado del frijol (BGYMV),en dicho estudio Rubí mostró resistencia al BCMV y baja incidencia de BGYMV.Durante 2015y 2016, Rubí,se evaluójunto con dos testigos regionales enun vivero de adaptación en seisambientes deVeracruz yChiapas, para determinar su respuesta productiva, bajo condiciones de temporal, humedad residual, riego y sequía terminal.En cuatro de los seisambientes,Rubífue superior en rendimiento a los dos testigos regionales, con un promedio de10.3 y 18.1%superioral obtenido porNegro Comapa y Negro Grijalva, respectivamente. Durante 2016 y 2017, Rubí se evaluó en un ensayo uniforme de rendimiento en10 ambientes de Veracruz y Chiapas. La nueva variedad obtuvoun rendimiento promedio sobresaliente ymostró mejor adaptación que Negro Comapa y Negro Grijalva.En 2019, el Servicio Nacional de Inspección y Certificación de Semillas (SNICS)otorgó el registro en el Catálogo Nacional de Variedades Vegetales, con número FRI-099-06119, para su uso comercial
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- 2022
8. Rendimiento y resistencia al mosaico amarillo dorado de genotipos de frijol negro tropical
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Tosquy Valle, Óscar Hugo, Villar Sánchez, Bernardo, Ibarra Pérez, Francisco Javier, Anaya López, José Luis, Garrido Ramírez, Eduardo, Tosquy Valle, Óscar Hugo, Villar Sánchez, Bernardo, Ibarra Pérez, Francisco Javier, Anaya López, José Luis, and Garrido Ramírez, Eduardo
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En las áreas tropicales de Chiapas, México, la producción de frijol es afectada por el mosaico amarillo dorado, la sequía y la acidez de los suelos. El objetivo de esta investigación fue identificar genotipos de frijol resistentes al mosaico amarillo dorado, con mayor productividad que la variedad Negro Jamapa, comúnmente sembrada en Chiapas. En otoño-invierno de 2019-2020 se estableció un experimento en tres localidades del centro de Chiapas, se evaluaron 11 líneas y tres variedades de frijol negro, incluida Negro Jamapa. El diseño experimental fue bloques al azar con tres repeticiones. Se cuantificó la incidencia de mosaico amarillo dorado y el rendimiento de grano. Los datos de las tres localidades se analizaron de manera individual y combinada. También se realizaron análisis de correlación entre la incidencia de mosaico amarillo dorado y el rendimiento de los genotipos. El mosaico amarillo dorado redujo el rendimiento en los tres sitios de evaluación (Villa Corzo, r= -0.562*; CECECH, r= -0.757** y El Gavilán, r= -0.552*). La línea Jamapa Plus/XRAV-187-3-4-1 mostró los mayores daños por esta enfermedad con una calificación promedio de incidencia de 6, significativamente superior al resto de los genotipos; en tanto que, las líneas: Negro Citlali/XRAV-187-3-1-6, Papaloapan/SEN 46-7-7 y Jamapa Plus/XRAV-187-3-4-4 y la variedad Verdín mostraron la mayor resistencia al mosaico amarillo dorado, con calificaciones de incidencia de entre 1.67 y 3.22, estadísticamente inferiores a la del testigo Negro Jamapa. Estos cuatro genotipos también obtuvieron rendimientos promedio significativamente sobresalientes (mayores a 934 kg ha-1) y superiores al de Negro Jamapa., In the tropical areas of Chiapas, Mexico, bean production is affected by golden yellow mosaic, droughtand soil acidity. The objective of this research was to identify bean genotypes resistant to the golden yellow mosaic, with higher productivity than the Negro Jamapa variety, commonly sownin Chiapas. Intheautumn-winterof2019-20 20, an experiment was established in three localities in central Chiapas,11 lines and three varieties of black bean, including Negro Jamapa, were evaluated. The experimental design was randomized blocks with three repetitions. The incidence of golden yellow mosaic and grain yield were quantified. Data from the three localitieswere analyzed individually and in combination. Correlation analyses between the incidence of golden yellow mosaic and genotype yieldwere also performed.The golden yellow mosaic significantly reduced yieldat the three sites of evaluation (Villa Corzo, r= -0.562*; CECECH, r= -0.757**and El Gavilán, r= -0.552*). The Jamapa Plus/XRAV-187-3-4-1 line showed the greatest damage from this disease with an average incidence scoreof 6, significantly higher than those of the rest of the genotypes, while the lines: NegroCitlali/XRAV-187-3-1-6, Papaloapan/SEN 46-7-7 andJamapa Plus/XRAV-187-3-4-4 and the Verdín variety showed the highest resistance to the golden yellow mosaic,with incidence scoresbetween 1.67 and 3.22, statistically lower than that of the controlNegro Jamapa. These four genotypes also obtained averageyields that were significantly outstanding (greater than 934 kg ha-1) and higher than that of Negro Jamapa.
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- 2022
9. Molecular characterisation of Jatropha curcas L. genetic resources from Chiapas, México through AFLP markers
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Pecina-Quintero, Víctor, Anaya-López, José Luis, Colmenero, Alfredo Zamarripa, García, Noe Montes, Núñez Colín, Carlos Alberto, Solis Bonilla, Jose Luis, Aguilar-Rangel, María Rocío, Gill Langarica, Homar Rene, and Méjia Bustamante, Daniela Judith
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- 2011
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10. RENDIMIENTO E ÍNDICE DE COSECHA DE GERMOPLASMA DE FRIJOL PINTO Y FLOR DE MAYO BAJO ESTRÉS POR TEMPERATURA ALTA
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Anaya-López, José Luis, primary, Rojas-Tovar, Luis Manuel, additional, Cisneros-López, Hugo César, additional, and Acosta-Gallegos, Jorge Alberto, additional
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- 2022
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11. Leguminosas de grano en México: variedades mejoradas de frijol y garbanzo desarrolladas por el INIFAP
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Anaya-López, José Luis, primary, Ibarra-Pérez, Francisco Javier, additional, Rodríguez-Cota, Franklin Gerardo, additional, Ortega-Murrieta, Pedro Francisco, additional, Acosta-Gallegos, Jorge Alberto, additional, and Chiquito-Almanza, Elizabeth, additional
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- 2021
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12. Leguminosas de grano en México: variedades mejoradas de frijol y garbanzo desarrolladas por el INIFAP
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Anaya López, José Luis, Ibarra Pérez, Francisco Javier, Rodríguez Cota, Franklin Gerardo, Ortega Murrieta, Pedro F, Chiquito Almanza, Elizabeth, Acosta Gallegos, Jorge Alberto, Anaya López, José Luis, Ibarra Pérez, Francisco Javier, Rodríguez Cota, Franklin Gerardo, Ortega Murrieta, Pedro F, Chiquito Almanza, Elizabeth, and Acosta Gallegos, Jorge Alberto
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Common bean, chickpea, broad bean, lentil and cowpeaare among the best known and most consumed grain legumes in the world. In Mexico, bean(Phaseolusspp.) is theone withhighest production and consumption. Of thosefive species, the National Institute of Forestry, Agricultural and Livestock Research has had a great participationin both the development of common bean varieties and large-grainedwhitechickpeaandsmall-grained brown chickpea.At the national level,somebeanvarietieshave become a benchmark for their yield and quality of their grain, such as ‘Negro Jamapa’,‘Mayocoba’, ‘Azufrado Higuera’,‘Flor de Junio Marcela’and ‘Pinto Saltillo’. In recent years,varieties of thetypes with the greatest demand are starting to be used, some developed using molecular markers in the selection process, as in the cases of ‘San Blas’and ‘Rubí’, resistant to BCMV and BCMNV. In the case of chickpea, for two decadesthe variety ‘Blanco Sinaloa-92’has been the dominant in the local and export markets, and new varieties of white grain are available for Sinaloa, Sonora and ElBajío.With the aim of publicizingINIFAP's contribution to the improvement of grain legumes in Mexico, thisessaypresents the organization ofthe INIFAP bean and chickpea improvement headquartersand the varieties they have developed and registered in the last 20 years, El frijol común, garbanzo, haba, lenteja y caupí están entre las leguminosas de grano más conocidas y consumidas en el mundo. En México el frijol (Phaseolusspp.) es el de mayor producción y consumo. De esas cinco especies, el Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias ha tenido una gran participación tanto en el desarrollo de variedades de frijol común como de garbanzo blanco de grano grande y de color café de grano pequeño. A nivel nacional algunas variedades de frijol se han convertido en un referente por su rendimiento y la calidad de su grano, como son ‘Negro Jamapa’, ‘Mayocoba’, ‘Azufrado Higuera’, ‘Flor de Junio Marcela’ y ‘Pinto Saltillo’. En años recientes se han registrado variedades de los tipos con mayor demanda que están comenzando a utilizarse, algunas desarrolladas usando marcadores moleculares en el proceso de selección, tal es el caso de ‘San Blas’y ‘Rubí’, resistentes al BCMV y BCMNV. En el caso del garbanzo, desde hace dos décadas la variedad ‘Blanco Sinaloa-92’es la dominante en el mercado local y de exportación,y se cuenta con nuevas variedades de grano blanco para Sinaloa, Sonora y el Bajío. Con el objetivo de dar a conocer la contribución del INIFAP al mejoramiento de leguminosas de grano en México, en este ensayo se presenta laorganización de las sedesde mejoramiento de frijol y garbanzo del INIFAPy las variedades que han desarrolladoy registradoen los últimos 20 años.
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- 2021
13. Diversity and Distribution of Viruses Infecting Wild and Domesticated Phaseolus spp. in the Mesoamerican Center of Domestication
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Chiquito-Almanza, Elizabeth, primary, Caballero-Pérez, Juan, additional, Acosta-Gallegos, Jorge A., additional, Montero-Tavera, Victor, additional, Mariscal-Amaro, Luis Antonio, additional, and Anaya-López, José Luis, additional
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- 2021
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14. San Blas: variedad de frijol Negro Opaco resistente a BCMV y BCMNV para Nayarit y El Bajío de Guanajuato
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Anaya López, José Luis, Acosta Gallegos, Jorge Alberto, Jiménez Hernández, Yanet, García Álvarez, Nadia Carolina, Rojas Tovar, Manuel, Chiquito Almanza, Elizabeth, Anaya López, José Luis, Acosta Gallegos, Jorge Alberto, Jiménez Hernández, Yanet, García Álvarez, Nadia Carolina, Rojas Tovar, Manuel, and Chiquito Almanza, Elizabeth
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A new variety of beans is described, ‘San Blas’, whose main characteristics are its type of grain in high demand in Mexico and its resistance to the common bean mosaic virus (BCMV) and the mosaic virus and common bean necrosis (BCMNV), since it has the combination of genes II+ bc-3. San Blas was derived from the cross between Jamapa Plus/XRAV-187-3. The final selection of the line that gave rise to San Blas was carried out by inoculation with the necrotic strain BCMNV NL-3, and genotyping with the molecular markers (MMs) SW13 and ENM, associated with genes IIand bc-3, respectively.The San Blas plant has an indeterminate, semi-erect type II habit, with lilac-purple flowers, straw-colored pods at maturity and Negro Opacograin, oval in shape and small in size similar to Jamapa. In the Bajío, its flowering and physiological maturity occur around 45 and 92 days after sowing (dds); while in Nayarit at 40 and 80 dds, respectively. After selection with MMs and inoculation, the average yield of San Blas in six different environments in Guanajuato was 1841 kg ha-1, 12% more than that of Negro8025; while in three residual moisture environments in Nayarit it was 1927 kg ha-1, surpassing the Jamapa and Coranay varieties by 50 and 36%, respectively., Se describe una nueva variedad de frijol, ‘San Blas’, cuyas características principales son su tipo de grano de alta demanda en México y su resistencia al virus del mosaico común del frijol (BCMV) y al virus del mosaico y la necrosis común de frijol (BCMNV), ya que posee la combinación de genes II + bc-3. San Blas se derivó de la cruza entre Jamapa Plus/XRAV-187-3. La selección final de la línea que dio origen a San Blas se realizó por inoculación con la cepa necrótica BCMNV NL-3, y la genotipificación con los marcadores moleculares (MMs) SW13 y ENM, asociados a los genes II y bc-3, respectivamente. La planta de San Blas es de hábito indeterminado semierecta tipo II, con flores de color lila-morado, vainas color paja en madurez y grano negro opaco, de forma ovalada y tamaño pequeño similar al de Jamapa. En El Bajío, su floración y madurez fisiológica ocurren alrededor de los 45 y 92 días después de la siembra (dds); mientras que en Nayarit a los 40 y 80 dds, respectivamente. Después de la selección con MMs e inoculación, el rendimiento promedio de San Blas en seis ambientes diversos en Guanajuato fue de 1841 kg ha-1, 12% más que el de Negro 8025; mientras que en tres ambientes de humedad residual en Nayarit fue de 1927 kg ha-1, superando a las variedades Jamapa y Coranay en 50 y 36%, respectivamente.
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- 2020
15. ‘San Blas’: variedad de frijol Negro Opaco resistente a BCMV y BCMNV para Nayarit y El Bajío de Guanajuato
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Anaya-López, José Luis, primary, Acosta-Gallegos, Jorge Alberto, additional, Jiménez-Hernández, Yanet, additional, García-Álvarez, Nadia Carolina, additional, Rojas-Tovar, Manuel, additional, and Chiquito-Almanza, Elizabeth, additional
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- 2020
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16. Adaptación de genotipos de frijol negro a diferentes ambientes de Veracruz y Chiapas
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Tosquy Valle, Óscar Hugo, Villar Sánchez, Bernardo, Rodríguez Rodríguez, José Raúl, Ibarra Pérez, Francisco Javier, Zetina Lezama, Rigoberto, Meza, Pablo Andrés, Anaya López, José Luis, Tosquy Valle, Óscar Hugo, Villar Sánchez, Bernardo, Rodríguez Rodríguez, José Raúl, Ibarra Pérez, Francisco Javier, Zetina Lezama, Rigoberto, Meza, Pablo Andrés, and Anaya López, José Luis
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In the present investigation, the main additive effects and multiplicative interaction (AMMI) model was used to determine the yield and stability of 12 lines and two varieties of opaque black beans, evaluated during 2016 and 2017, in 10 environments of Veracruz and Chiapas, Mexico. The trial was established in experimental design random blocks with three repetitions and plots of three rows of 5 m in length. The grain yield was quantified, which was analyzed individually by environment and combined (environments-genotypes) of the 10 test environments, stability parameters were also estimated with the AMMI model. The Experimental Field Ixtacuaco and Rincon Grande, Veracruz, in autumn-winter 2016-2017 under residual humidity, were the environments that combinedlow interaction and high productivity, making them ideal for identifying genotypes with high and stable performance. Venustiano Carranza, Chiapas, in autumn-winter of 2016-2017, El Rubi, Veracrus, in winter-spring of 2017 with irrigation and New Mexico, Chiapas, in summer of 2016, in whitewashed acid soil, were the environments that more interacted with genotypes. The Jamapa Plus/XRAV-187-3-1-8 line showed the least interaction with the environment (much higher than that shown by the Negro Comapa and NegroGrijalva varieties), as well as highaverage yield (1 437.3 kg ha-1), while Jamapa Plus/XRAV-187-3-1-2, was the most profitable line (1 504.3 kg ha-1), but its adaptation was specific, mainly in environments with edaphic acid stress in the center from Chiapas and by terminal drought in the center of Veracruz., En la presente investigación se utilizó el modelo efectos principales aditivos e interacción multiplicativa (AMMI) para determinar el rendimiento y estabilidad de 12 líneas y dos variedades de frijol negro opaco, evaluadas durante 2016 y 2017, en 10 ambientes de Veracruz y Chiapas, México. El ensayo se estableció en diseño experimental bloques al azar con tres repeticiones y parcelas de tres surcos de 5 m de longitud. Se cuantificó el rendimiento de grano, el cual se analizó de manera individual por ambiente y combinada (ambientes-genotipos) de los 10 ambientes de prueba, también se estimaron los parámetros de estabilidad con el modelo AMMI. El Campo Experimental Ixtacuaco y Rincón Grande, Veracruz. en otoño-invierno de 2016-2017 bajo humedad residual, fueron los ambientes que combinaron baja interacción y alta productividad, por lo que son ideales para identificar genotipos con alto y estable rendimiento. Venustiano Carranza, Chiapas, en otoño-invierno de 2016-2017, El Rubí, Veracruz, en invierno-primavera de 2017 con riego y Nuevo México, Chiapas, en verano de 2016, en suelo ácido encalado, fueron los ambientes que más interaccionaron con los genotipos. La línea Jamapa Plus/XRAV-187-3-1-8, mostró la menor interacción con el ambiente (muy superior a las variedades Negro Comapa y Negro Grijalva), así como alto rendimiento (1 437.3 kg ha-1), mientras que, Jamapa Plus/XRAV-187-3-1-2, fue la línea más rendidora (1 504.3 kg ha-1), pero su adaptación fue específica, en los ambientes con estrés por acidez edáfica en el centro de Chiapas y por sequía terminal en el centro de Veracruz.
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- 2019
17. Identificación de líneas recombinantes de frijol negro opaco resistentes a BCMV, BCMNV y BGYMV mediante marcadores moleculares
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Anaya López, José Luis, Garrido Ramírez, Eduardo, Chiquito Almanza, Elizabeth, Tosquy Valle, Óscar Hugo, Ibarra Pérez, Francisco Javier, López Salinas, Ernesto, Anaya López, José Luis, Garrido Ramírez, Eduardo, Chiquito Almanza, Elizabeth, Tosquy Valle, Óscar Hugo, Ibarra Pérez, Francisco Javier, and López Salinas, Ernesto
- Abstract
The opaque black bean from the Mesoamerican collection is consumed and produced in Mexico. To identify recombinant lines of opaque black bean resistant to bean common mosaic virus (BCMV), mosaic virus and common bean necrosis (BCMNV) and bean golden yellow mosaic virus (BGYMV), during 2015 and 2016 a collection of 70 genotypes that included 20 varieties and 50 recombinant lines (LR) was evaluated. The LRs derived from three different crosses: ‛Negro Papaloapan’/SEN-46, ‛Negro Citlali’/XRAV-187-3 and ‛Jamapa Plus’/XRAV-187-3. All genotypes were inoculated with the strains BCMNV NL-3 and BGYMV-MX, and were genotyped with the molecular markers SW13, ENM, SBD5 and SR2 linked to the resistance genes I, bc-3, bc12and bgm-1.The 21 LR were identified with plants that had the genes bgm-1,I, or the combination I+ bc-3 and broad-spectrum or specific resistance to BCMV, BCMNV and BGYMV. All the LRs had a high proportion of plants with the Igene. Six LRs derived from the cross ‛Negro Papaloapan’/SEN-46 and two from eachof the crosses ‛Negro Citlali’/XRAV-187-3 and ‛Jamapa Plus’/XRAV-187-3 had between 8% and 92% of plants with the genetic combination I+ bc-3that confers resistance to BCMV and BCMNV. While the genes Iand bgm-1, which confers resistance to BCMV and BGYMV, were detected in three LRs of the ‛Negro Papaloapan’/SEN-46 and four of ‛Negro Citlali’/XRAV-187-3 crosses. Four LR derived fromthe cross ‛Negro Papaloapan’/SEN-46: ‛Negro Papaloapan’/SEN 46-7-8, ‛Negro Papaloapan’/SEN-46-7-11, ‛Negro Papaloapan’/SEN-46-7 -12 and ‛Negro Papaloapan’/SEN-46-7-13 had plants with the I, bc-3and bgm-1genes that confers broad-spectrum resistance to BCMV, BCMNV and BGYMV. The heterogeneity of the LR in the proportion of plants with the resistance genes, highlights the need to implement selection strategies assisted by molecular markers to homogenize them, and develop opaque black bean varieties with resistance to three of the viruses that most affect bean production in Latin America. Key, El frijol negro opaco de acervo mesoamericano se consume y produce en México. Con el objetivo de identificar líneas recombinantes de frijol negro opaco resistentes al virus del mosaico (BCMV), al virus del mosaico y la necrosis (BCMNV) y al virus del mosaico amarillo dorado del frijol (BGYMV), durante 2015 y 2016 se evaluó una colección de 70 genotipos que incluyeron 20 variedades y 50 líneas recombinantes (LR). Las LR derivaron de tres diferentes cruzas: ‘Negro Papaloapan’/SEN-46, ‘Negro Citlali’/XRAV-187-3 y ‘Jamapa Plus’/XRAV-187-3. Los genotipos se inocularon con las cepas BCMNV NL-3 y BGYMV-MX, y se genotipificaron con marcadores moleculares SW13, ENM, SBD5 y SR2 ligados a genes de resistencia I, bc-3, bc12 y bgm-1. Se identificaron 21 LR que tuvieron los genes bgm-1, I, o la combinación I + bc-3 y resistencia de amplio espectro a BCMV, BCMNV y BGYMV. Las LR tuvieron alta proporción de plantas con el gen I. Seis LR que derivaron de la cruza ‘Negro Papaloapan’/SEN-46 y dos de cada una de las cruzas ‘Negro Citlali’/XRAV-187-3 y ‘Jamapa Plus’/XRAV-187-3 tuvieron entre 8% y 92% de plantas con combinación genética I + bc-3 que confiere resistencia a BCMV y BCMNV. Mientras que los genes I y bgm-1, dan resistencia a BCMV y BGYMV, se detectaron en tres LR de la cruza ‘Negro Papaloapan’/SEN-46 y cuatro de ‘Negro Citlali’/XRAV-187-3. Cuatro LR derivadas de la cruza ‘Negro Papaloapan’/SEN-46: ‘Negro Papaloapan’/SEN 46-7-8, ‘Negro Papaloapan’/SEN- 46-7-11, ‘Negro Papaloapan’/SEN-46-7-12 y ‘Negro Papaloapan’/SEN-46-7-13 tuvieron plantas con genes I, bc-3 y bgm-1 con resistencia de amplio espectro a BCMV, BCMNV y BGYMV. La heterogeneidad de LR en proporción de plantas con genes de resistencia, surge la estrategia de selección asistida por marcadores moleculares para homogenizarlas y desarrollar variedades de frijol negro opaco con resistencia a tres virus que afectan la producción de frijol en América Latina. Palabras clave: Phaseolus vulgaris L., bc-3, bgm-1, e
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- 2018
18. Salinas: nueva variedad de frijol pinto para áreas de temporal de Guanajuato
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Jiménez-Hernández, Yanet, primary, Montero-Tavera, Víctor, additional, Anaya-López, José Luis, additional, Jiménez-Galindo, José Cruz, additional, Herrera-Hernández, Guadalupe, additional, Guerrero-Aguilar, Brenda Zulema, additional, and Acosta-Gallegos, Jorge Alberto, additional
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- 2018
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19. Identificación de líneas recombinantes de frijol negro opaco resistentes a BCMV, BCMNV y BGYMV mediante marcadores moleculares
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Anaya-López, José Luis, primary, Garrido-Ramírez, Eduardo Raymundo, additional, Chiquito-Almanza, Elizabeth, additional, Tosquy-Valle, Oscar Hugo, additional, Ibarra-Pérez, Francisco Javier, additional, and López-Salinas, Ernesto, additional
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- 2018
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20. Diversidad genética de aislados de Rhizoctonia solani (Kuhn) de chile en México
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Montero-Tavera, Víctor, primary, Guerrero-Aguilar, Brenda Zulema, additional, Anaya-López, José Luis, additional, Martínez-Martínez, Talina Olivia, additional, Olvera Lorenzo, Guevara-, additional, and González-Chavira, Mario Martín, additional
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- 2018
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21. Análisis de la calidad sanitaria de nopal verdura en Otumba, Estado de México
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Angeles-Núñez, Juan Gabriel, primary, Anaya-López, José Luis, additional, Arévalo-Galarza, Ma. de Lourdes, additional, Leyva-Ruelas, Gabriel, additional, and Martínez-Martínez, Talina Olivia, additional
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- 2018
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22. ‘Dalia’, nueva variedad de frijol de grano tipo Flor de Junio para la región centro de México
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Acosta-Gallegos, Jorge Alberto, primary, Montero-Tavera, Víctor, additional, Jiménez-Hernández, Yanet, additional, Anaya-López, José Luis, additional, and Gonzalez-Chavira, Mario Martín, additional
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- 2018
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23. Estudio regional de fitopatógenos asociados a la secadera del chile en Guanajuato, México
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Albañil Juárez, José Alejandro, primary, Mariscal Amaro, Luis Antonio, additional, Martínez Martínez, Talina Olivia, additional, Anaya López, José Luis, additional, Cisneros López, Hugo César, additional, and Pérez Ramírez, Hugo Armando, additional
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- 2018
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24. Retos y oportunidades en la selección asistida de frijol resistente a BCMV y BCMNV en México. II. Oportunidades para la selección asistida
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Anaya-López, José Luis, primary, Espejel, Fulgencio, additional, Montero-Tavera, Víctor, additional, Acosta-Gallegos, Jorge Alberto, additional, and Silva-Rosales, Laura, additional
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- 2017
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25. Retos y oportunidades en la selección asistida de frijol resistente a BCMV y BCMNV en México. I. Dimensión del problema
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Anaya-López, José Luis, primary, Silva-Rosales, Laura, additional, Montero-Tavera, Víctor, additional, Espejel, Fulgencio, additional, and Acosta-Gallegos, Jorge Alberto, additional
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- 2017
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26. San Rafael, nueva variedad de frijol pinto de reacción neutral al fotoperiodo para el centro de México
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Acosta Gallegos, Jorge A., primary, Jiménez Hernández, Yanet, additional, Montero Tavera, Víctor, additional, Guzmán Maldonado, S. Horacio, additional, and Anaya López, José Luis, additional
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- 2017
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27. Retos y oportunidades en la selección asistida de frijol resistente a BCMV y BCMNV en México. II. Oportunidades para la selección asistida
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Anaya López, José Luis, Espejel, Fulgencio, Montero Tavera, Víctor, Acosta Gallegos, Jorge Alberto, Silva Rosales, Laura, Anaya López, José Luis, Espejel, Fulgencio, Montero Tavera, Víctor, Acosta Gallegos, Jorge Alberto, and Silva Rosales, Laura
- Abstract
Considering recent information, the objective was to review the knowledge on the mechanisms of resistance to BCMV and BCMNV, describe the use of DNA markers for assisted selection of bean varieties resistant to both viruses and point out the need to design and generate more efficient molecular markers. The most economical and efficient way to prevent damage caused by both viruses is to plant resistant varieties obtained by gene pyramiding, in which a broad-spectrum resistance against pathogroups of BCMV and BCMNV are combined. For over 30 years the role of the dominant I gene are known, which confers resistance to non-necrotic strains, and bc recessive genes, including c-u, bc-1, bc-12, bc-2, bc-22, and bc-3, which confer resistance to some specific pathogroups of BCMV and BCMNV. However, except for bc-3 gene which corresponds to PveIF4E gene, their identity is unknown. Regarding to bc3 gene has been identified at least three alleles, bc31, bc32 and bc33, of which only the bc32 allele in combination with I gene, confers immunity to strain of NL3 from BCMNV. To date, the two most used molecular markers to monitor BCMV resistance: ROC11 and SW13 have not been consistent through diverse germplasm. It is therefore necessary to develop new or additional resistance markers based on knowledge of the biological function of the corresponding genes., Considerando información reciente, el objetivo fue revisar el conocimiento de los mecanismos de resistencia a BCMV y BCMNV, describir el uso de marcadores de ADN para la selección asistida de variedades de frijol resistentes a ambos virus y señalar la necesidad de diseñar y generar marcadores moleculares más eficientes. La forma más económica y eficiente para prevenir los daños causados por ambos virus es sembrar variedades resistentes obtenidas mediante la piramidación de genes, en la que se combine una resistencia de amplio espectro contra los patogrupos de BCMV y BCMNV. Desde hace más de 30 años se conoce la función del gen dominante I, que confiere resistencia a cepas no necróticas, y de los genes recesivos bc, que incluyen bc-u, bc-1, bc-12, bc-2, bc-22, y bc-3, que confieren resistencia específica a algunos patogrupos de BCMV y BCMNV. Sin embargo, a excepción del gen bc-3 que corresponde al gen PveIF4E, se desconoce la identidad de todos ellos. En relación al gen bc3 se han identificado al menos tres alelos, bc31, bc32 y bc33, de los cuales solo el alelo bc32, en combinación con el gen I, confiere inmunidad a la cepa NL3 de BCMNV. Hasta el presente, los dos marcadores moleculares más utilizados para monitorear resistencia a BCMV: ROC11 y SW13 no han sido consistentes a través de germoplasma diverso. Por esta razón es necesario desarrollar nuevos o marcadores de resistencia adicionales basados en el conocimiento de la función biológica de los genes correspondientes.
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- 2015
28. Retos y oportunidades en la selección asistida de frijol resistente a BCMV y BCMNV en México. I. Dimensión del problema
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Anaya López, José Luis, Silva Rosales, Laura, Montero Tavera, Víctor, Espejel, Fulgencio, Acosta Gallegos, Jorge Alberto, Anaya López, José Luis, Silva Rosales, Laura, Montero Tavera, Víctor, Espejel, Fulgencio, and Acosta Gallegos, Jorge Alberto
- Abstract
The bean diseases known as common mosaic and black root, caused by bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) have increased incidence in Mexico. The BCMV is widely distributed in almost all production regions, and BCMNV preferably in tropical regions. Both viruses cause similar symptoms and can be transmitted by aphids or seed from infected plants, so that its incidence is related to the cultivation of susceptible varieties and climatic conditions that favor the presence of vectors. Control of BCMV and BCMNV can be achieved through certification programs of disease-free seed and use of resistant varieties. The cheapest way to prevent damage is to plant varieties with durable resistance obtained by gene pyramiding, in which a broad-spectrum of resistance is combined against BCMV and BCMNV. This breeding process can be facilitated using molecular marker assisted selection in combination with direct confrontation of the virus. The objective is to elucidate the issues surrounding the impact of BCMV and BCMNV in major producing areas of bean to identify challenges and opportunities in assisted selection of beans resistant to BCMV and BCMNV., Las enfermedades del frijol conocidas como mosaico común y raíz negra, causadas por el virus del mosaico común del frijol (BCMV) y el virus necrótico del mosaico común del frijol (BCMNV), tienen cada vez mayor incidencia en México. El BCMV está ampliamente distribuido en casi todas las regiones productoras, y el BCMNV preferentemente en las regiones tropicales. Ambos virus producen síntomas similares y pueden transmitirse por áfidos o por semilla proveniente de plantas infectadas, por lo que su incidencia está relacionada con el cultivo de variedades susceptibles y las condiciones climáticas que favorecen la presencia de insectos vectores. El control de BCMV y BCMNV puede lograrse mediante programas de certificación de semilla libre de enfermedades y el uso de variedades resistentes. La forma más económica de prevenir daños es sembrar variedades con resistencia durable obtenida mediante la piramidación de genes, en la que se combina una resistencia de amplio espectro contra patogrupos de BCMV y BCMNV. Este proceso de mejoramiento puede facilitarse mediante el uso de selección asistida por marcadores moleculares en combinación con la confrontación directa de los virus. El objetivo es dilucidar la problemática en torno a la incidencia del BCMV y BCMNV en las principales regiones productoras de frijol para identificar los retos y oportunidades en la selección asistida de frijol resistente a BCMV y BCMNV.
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- 2015
29. Salinas: new variety of pinto beans for rainfed areas of Guanajuato.
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Jiménez-Hernández, Yanet, Montero-Tavera, Víctor, Anaya-López, José Luis, Jiménez-Galindo, José Cruz, Herrera-Hernández, Guadalupe, Guerrero-Aguilar, Brenda Zulema, and Acosta-Gallegos, Jorge Alberto
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PLANT growth ,PINTO bean ,MOSAIC diseases ,PLANT diseases - Abstract
Salinas is a variety of pinto beans, indeterminate-prostrate type III, short-day photoperiod, early maturity, rust-resistant, halo blight and anthracnose, slow darkening grain and high hydration capacity. It was developed by the Bajío Experimental Bean Program (CEBAJ). It was derived from the retro-cross of Pinto Saltillo// (Pinto Saltillo/97-RS
01) and its evaluation started as line F9 developed by pedigree selection. In later generations, it was evaluated based on yield and resistance to diseases in different places of the Semi-arid Highlands and Central Mexico. In three years of trial (2010-2012) in rainfed environments showed similar performance potential and was superior in disease resistance to Pinto Saltillo. Salinas carries molecular markers of genes associated with rust resistance (Ur3-Ur7, Ur9 and GB), anthracnose (Co1, Co1-1 2 , Co2, Co4, Co42 and Co6) and halo blight (SR13, ST8, SAE15 and SW13) and in the field, shows resistance to the races of these prevalent diseases in Guanajuato. It is also resistant to common mosaic virus (BCMV) due to the presence of the gen I. Salinas gene produces medium-sized grain of mottled pinto type (33 ±3 g 100 seeds ). The average yield in rainfed season depends on the environment and the agronomic management, in the localities of Guanajuato it goes from 622 kg ha-1 in San Luis of the Paz, to 3 209 kg ha-1 in Salvatierra with optimal fertilization and sowing date. [ABSTRACT FROM AUTHOR]-1 - Published
- 2018
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30. Identification of opaque black bean recombinant lines resistant to BCMV, BCMNV and BGYMV using molecular markers.
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Anaya-López, José Luis, Garrido-Ramírez, Eduardo Raymundo, Chiquito-Almanza, Elizabeth, Tosquy-Valle, Oscar Hugo, Ibarra-Pérez, Francisco Javier, and López-Salinas, Ernesto
- Subjects
BLACK bean ,NECROSIS ,CULTIVARS ,MOSAIC viruses ,PLANT genetics - Abstract
The opaque black bean from the Mesoamerican collection is consumed and produced in Mexico. To identify recombinant lines of opaque black bean resistant to bean common mosaic virus (BCMV), mosaic virus and common bean necrosis (BCMNV) and bean golden yellow mosaic virus (BGYMV), during 2015 and 2016 a collection of 70 genotypes that included 20 varieties and 50 recombinant lines (LR) was evaluated. The LRs derived from three different crosses: ‛Negro Papaloapan'/SEN-46, ‛Negro Citlali'/XRAV-187-3 and ‛Jamapa Plus'/XRAV-187-3. All genotypes were inoculated with the strains BCMNV NL-3 and BGYMV-MX, and were genotyped with the molecular markers SW13, ENM, SBD5 and SR2 linked to the resistance genes I, bc-3, bc12 and bgm-1. The 21 LR were identified with plants that had the genes bgm-1, I, or the combination I + bc-3 and broad-spectrum or specific resistance to BCMV, BCMNV and BGYMV. All the LRs had a high proportion of plants with the I gene. Six LRs derived from the cross ‛Negro Papaloapan'/SEN-46 and two from each of the crosses ‛Negro Citlali'/XRAV-187-3 and ‛Jamapa Plus'/XRAV-187-3 had between 8% and 92% of plants with the genetic combination I + bc-3 that confers resistance to BCMV and BCMNV. While the genes I and bgm-1, which confers resistance to BCMV and BGYMV, were detected in three LRs of the ‛Negro Papaloapan'/SEN-46 and four of ‛Negro Citlali'/XRAV-187-3 crosses. Four LR derived from the cross ‛Negro Papaloapan'/SEN-46: ‛Negro Papaloapan'/SEN 46-7-8, ‛Negro Papaloapan'/SEN-46-7-11, ‛Negro Papaloapan'/SEN-46-7 -12 and ‛Negro Papaloapan'/SEN 46-7-13 had plants with the I, bc-3 and bgm-1 genes that confers broad-spectrum resistance to BCMV, BCMNV and BGYMV. The heterogeneity of the LR in the proportion of plants with the resistance genes, highlights the need to implement selection strategies assisted by molecular markers to homogenize them, and develop opaque black bean varieties with resistance to three of the viruses that most affect bean production in Latin America. [ABSTRACT FROM AUTHOR]
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- 2018
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31. Genetic diversity of sweet sorghum germplasm in Mexico using AFLP and SSR markers
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Pecina‑Quintero, Víctor, Anaya‑López, José Luis, Zamarripa‑Colmenero, Alfredo, Montes‑García, Noe, Nuñez‑Colín, Carlos, Solis‑Bonilla, Jose Luis, Aguilar‑Rangel, María Rocío, and Prom, Louis
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molecular marker ,rare allele ,genetic improvement ,alelos raros ,melhoramento genético ,biocombustíveis ,marcador molecular ,Sorghum bicolor ,biofuels - Abstract
The objective of this work was to evaluate the diversity and genetic relationships between lines and varieties of the sweet sorghum (Sorghum bicolor) germplasm bank of the National Institute for Forestry, Agriculture and Livestock Research, Mexico, using AFLP and SSR markers. The molecular markers revealed robust amplification profiles and were able to differentiate the 41 genotypes of sweet sorghum evaluated. Analysis of the frequency and distribution of polymorphic fragments allowed for the detection of unique (AFLP) and rare (SSR) alleles in several genotypes (RBSS‑8, RBSS‑9, RBSS‑25, RBSS‑32, and RBSS‑37), indicating that these markers may be associated with a feature that has not yet been determined or may be useful for the identification of these genotypes. The genetic relationships indicated the presence of at least two types of sweet sorghum: a group of modern genotypes used for sugar and biofuel production, and another group consisting of historic and modern genotypes used for the production of syrups. Sweet sorghum genotypes may be used to develop new varieties with higher sugar and juice contents. O objetivo deste trabalho foi avaliar a diversidade e as relações genéticas entre linhagens e variedades de sorgo sacarino (Sorghum bicolor) do banco de germoplasma do Instituto Nacional de Investigações Florestais, Agrícolas e Pecuárias, México, por meio de marcadores AFLP e SSR. Os marcadores moleculares revelaram robustez dos perfis de amplificação e permitiram diferenciar os 41 genótipos de sorgo sacarino avaliados. A análise da frequência e da distribuição dos fragmentos polimórficos permitiu detectar alelos únicos (AFLP) e raros (SSR) em diversos genótipos (RBSS‑8, RBSS‑9, RBSS‑25, RBSS‑32 e RBSS‑37), o que indica que estes marcadores podem estar associados a uma característica que ainda não foi determinada ou podem ser úteis para a identificação destes genótipos. As relações genéticas indicaram a presença de pelo menos dois tipos de sorgo sacarino: um grupo de genótipos modernos usados para a produção de açúcar e biocombustíveis, e outro grupo formado por genótipos antigos e modernos utilizados para a produção de xaropes. Genótipos de sorgo sacarino podem ser usados para o desenvolvimento de novas variedades com maior conteúdo de açúcar e caldo.
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- 2012
32. Caracterización Genética deSpodoptera frugiperda(Lepidoptera: Noctuidae) en México Usando Marcadores AFLP
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Pecina-Quintero, Víctor, primary, Anaya-López, José Luis, additional, Cortez-Mondaca, Edgardo, additional, Nuñez-Colín, Carlos Alberto, additional, Herrera-Corredor, Carlos, additional, Montes-García, Noé, additional, Jimenez-Becerril, Maria Fernanda, additional, and Gámez-Vazquez, Alfredo Josué, additional
- Published
- 2015
- Full Text
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33. 'Dalia', nueva variedad de frijol de grano tipo Flor de Junio para la región centro de México
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Acosta Gallegos, Jorge Alberto, Montero Tavera, Víctor, Jiménez Hernández, Yanet, Anaya López, José Luis, González Chavira, Mario M., Acosta Gallegos, Jorge Alberto, Montero Tavera, Víctor, Jiménez Hernández, Yanet, Anaya López, José Luis, and González Chavira, Mario M.
- Abstract
Flor de Junio has grains of cream colored background with pink stripes; it is produced and consumed in the central region of Mexico. We describe the process for its gathering and Dalias features, a new variety of bean grain, Flor de Junio type, Jalisco race, obtained from the cross of Flor de Junio Silvia X Flor de Mayo Anita performed in the Bajío Experimental Field in 2003. The selection process was initially based on health and the cycle of the plant, as well as the commercial properties in grain crops under alternative irrigation and rainfed conditions. In its final phase, we conducted an assisted molecular marker selection (AMS) for selecting the offspring that had simultaneously the markers ROC11 and SW13, linked to genes I and bc3, whose pyramiding confers resistance to all pathotypes of BCMV and BCMNV viruses. Dalia has an indeterminate growth habit type 3, with medium size guides and intermediate growing season with 105 days from sowing for physiological maturity and is resistant to BCMV and BCMNV. In a national assessment of yield and adaptation, Dalia showed similar yielding of Flor de Junio Marcela, the dominant variety within the market, but contrasting with this, a none existent G*E interaction, which indicated a high adaptation and yield stability. Dalia is recommended for irrigated and rainfed production in the regions of El Bajío and highlands of central Mexico., El frijol tipo Flor de Junio, cuyo color de grano es de fondo crema con franjas rosas, se produce y consume en la región Centro de México. Se describe el proceso de obtención y características de Dalia, una nueva variedad de frijol tipo Flor de Junio de raza Jalisco, derivada de la cruza de Flor de Junio Silvia por Flor de Mayo Anita realizada en el Campo Experimental Bajío en 2003. El proceso de selección se basó inicialmente en la sanidad y el ciclo de la planta, así como en las características comerciales del grano en siembras bajo condiciones alternas de riego y temporal. En su fase final se realizó selección asistida por marcadores moleculares (SAM) para seleccionar la progenie que tuviera simultáneamente los marcadores ROC11 y SW13, ligados a los genes I y bc3, cuya piramidación confieren resistencia a todos los patotipos de los virus BCMV y BCMNV. Dalia es de hábito de crecimiento indeterminado tipo 3, con guías de tamaño medio y ciclo de cultivo intermedio de 105 días de siembra a la madurez fisiológica, y resistente a BCMV y BCMNV. En un ensayo nacional de rendimiento y adaptación, Dalia mostró un rendimiento similar a Flor de Junio Marcela, la variedad dominante en el mercado, pero en contraste con ésta, una nula interacción genotipo por ambiente, lo que indicó amplia adaptación y estabilidad de rendimiento. Dalia se recomienda para producción bajo riego y temporal en las regiones de El Bajío y el Altiplano del centro de México.
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- 2014
34. Diversidad genética de aislados de 'Rhizoctonia solani' (Kuhn) de chile en México
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Montero Tavera, Víctor, Guerrero Aguilar, Brenda Zulema, Anaya López, José Luis, Martínez Martínez, Talina Olivia, Guevara Olvera, Lorenzo, González Chavira, Mario M., Montero Tavera, Víctor, Guerrero Aguilar, Brenda Zulema, Anaya López, José Luis, Martínez Martínez, Talina Olivia, Guevara Olvera, Lorenzo, and González Chavira, Mario M.
- Abstract
One of the major constraints for the production of pepper are pathogenic fungi causing diseases known as "pepper blight" or "damping off". This disease can be devastating when weather conditions are favorable for the pathogen. Although different means of control (chemical and cultural) have benn used but none has been successful. An alternative to control is to produce resistant germplasm, however in order to establish an effective breeding program is necessary to know the distribution and genetic diversity of the pathogens involved, particularly Rhizoctonia solani, which by its ubiquity represents a potential danger in all producing areas. Thus, the objective was to characterize R. solani in North Central area from Mexico and determine its genetic diversity. To achieve with this goal are considered the states of Chihuahua, Durango, Zacatecas, San Luis Potosi, Colima, Queretaro and Guanajuato where in 2009 were collected adult plants of pepper with pepper blight symptoms, the fungus was isolated and found an incidence of 33%, finding it in both stem and root. Mycelial cells were multinucleated, a characteristic from pathogenic strains. The anastomosis testing showed that in Mexico are present the groups GA4, GA-2. 1, GA-IIB, GA-2IV, GA7, GA11, GA12 and GA13. The genetic diversity of this fungus was very high, so that the relationships demonstrated by the construction of dendrogram show no homogeneous trends so as the main groups formed contain elements of all states, Una de las principales limitantes para la producción de chile son los hongos patógenos causantes de la enfermedad conocida como "marchitez del chile" o "secadera". Esta enfermedad puede ser devastadora cuando las condiciones climáticas son favorables para el patógeno. A pesar de que se han intentado diferentes medios de control (químicos y culturales) ninguno ha tenido éxito. Una alternativa para su control es producir germoplasma resistente, sin embargo para poder establecer un programa de mejoramiento efectivo es necesario conocer la distribución y diversidad genética de los patógenos involucrados, particularmente de Rhizoctonia solani, que por su ubicuidad representa un peligro potencial en todas las zonas productoras. Por ello el objetivo fue caracterizar a R. solani en las zona Centro Norte de México y determinar su diversidad genética. Para cumplir con este objetivo se consideraron los estados de Chihuahua, Durango, Zacatecas, San Luis Potosí, Colima, Querétaro y Guanajuato donde en 2009 se colectaron plantas adultas de Chile con síntomas de marchitez, se aisló al hongo y se encontró una incidencia del 33%, encontrándose tanto en tallo como en raíz. Las células miceliales fueron multinucleadas, características de las cepas patogénicas. Las pruebas de anastomosis demostaron la presencia en México de los grupos GA4, GA-2.1, GA-IIB, GA-2IV, GA7, GA11, GA12 y GA13. La diversidad genética de este hongo fue muy alta, de tal manera que las relaciones demostradas por la construcción de dendrogramas no muestran tendencias homogéneas pues los principales grupos formados contienen elementos de todos los estados
- Published
- 2013
35. Resultados de un control de calidad de datos de temperatura superficial del aire y humedad relativa
- Author
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Anaya López, José Luis and Anaya López, José Luis
- Abstract
Se presenta un estudio básico sobre control de calidad de datos de temperatura y humedad relativa. Este trabajo fue efectuado en el Instituto Meteorológico Nacional (IMN) de Costa Rica. El objetivo fue determinar de una forma objetiva, cual es la calidad de los datos generados por la red de estaciones meteorológicas. Los programas de control de calidad marcaron datos sospechosos y erróneos de temperatura que pasaron desapercibidos en los niveles anteriores del control de calidad. Los resultados muestran que existen valores atípicos infiltrados que escaparon a la detección del método de revisión manual tradicionalmente aplicado. De forma general, el porcentaje de valores atípicos de humedad relativa fue mayor que el encontrado para datos de temperatura. Dicho porcentaje nunca fue mayor al 20% del total de datos de la serie cronológica correspondiente. Los resultados de este trabajo también muestran cómo los programas desarrollados mejoran la eficiencia del protocolo actual de control de calidad.
- Published
- 2011
36. Selección de genotipos de chile resistentes al complejo patogénico de la marchitez
- Author
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Anaya López, José Luis, González Chavira, Mario M., Villordo Pineda, Emiliano, Rodríguez-Guerra, Raúl, Rodríguez Martínez, Raúl, Guevara González, Ramón G., Guevara Olvera, Lorenzo, Montero Tavera, Víctor, Torres Pacheco, Irineo, Anaya López, José Luis, González Chavira, Mario M., Villordo Pineda, Emiliano, Rodríguez-Guerra, Raúl, Rodríguez Martínez, Raúl, Guevara González, Ramón G., Guevara Olvera, Lorenzo, Montero Tavera, Víctor, and Torres Pacheco, Irineo
- Abstract
In Mexico the most important root disease of the chili pepper crop is the wilt disease, it is primarily controlled with fumigants and fungicides that help to select resistant isolates and cause environment and health damage.A safe environmental option could be the resistant varieties cultivation, but there are few disease resistance varieties. This study's aim was to isolate the pathogens associated with chili pepper wilt disease in central and north of Mexico and to identify chili pepper resistant genotypes. During 2006 and 2007, chili pepper plants with wilt disease symptoms were collected in 118 lots from Chihuahua, Colima, Durango, Guanajuato, Querétaro, San Luis Potosí and Zacatecas, from which pathogens were isolated and pure cultures were obtained. They were individually or in mixtures inoculated to select resistant germplasm, in 44 chili pepper accessions of INIFAP's germplasm bank and 141 collections from Durango, Guanajuato, Michoacán, San Luis Potosí and Zacatecas. Fusarium spp., was isolated with a 42.6% frequency, Rhizoctonia solani 37% and Phytophthora capsici 3.9%. 26 collections were identified with at least one of them resistant to Fusarium spp., and six to R. solani. Only BG102 and BG107 accessions from the gene bank wereresistant to P. capsici and to the group of three pathogens. These are potential materials to be used in chili pepper genetic improvement., En México la enfermedad de raíz más importante del cultivo de chile es la marchitez, esta se controla principalmente con fumigantes y fungicidas que contribuyen a seleccionar aislados resistentes, y provocan daños al ambiente y a la salud. Una opción inocua con el ambiente podría ser el cultivo de variedades resistentes; sin embargo, hay pocas variedades con resistencia a esta enfermedad. El objetivo del presente trabajo fue aislar los patógenos asociados a la marchitez del chile, en la región centro y norte de México e identificar genotipos de chile resistentes. Durante 2006 y 2007, se colectaron plantas de chile con síntomas de marchitez en 118 lotes de los estados de Chihuahua, Colima, Durango, Guanajuato, Querétaro, San Luis Potosí y Zacatecas, a partir de las cuales se aislaron los patógenos y se obtuvieron cultivos puros. Estos se inocularon individualmente o en mezcla para seleccionar germoplasma resistente en 44 accesiones de chile del banco de germoplasma del INIFAP y 141 colectas procedentes de Durango, Guanajuato, Michoacán, San Luis Potosí y Zacatecas. Fusarium spp. fue aislado con una frecuencia de 42.6%, Rhizoctonia solani 37%, y Phytophthora capsici 3.9%. Se identificaron 26 colectas con al menos un individuo resistente a Fusarium spp., y seis a R. solani. Sólo las accesiones BG102 y BG107 del banco de germoplasma fueron resistentes a P. capsici y a la mezcla de los tres patógenos. Estos materiales tienen potencial para usarse en programas de mejoramiento genético del chile.
- Published
- 2011
37. Genetic diversity of sweet sorghum germplasm in Mexico using AFLP and SSR markers
- Author
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Pecina‑Quintero, Víctor, primary, Anaya‑López, José Luis, additional, Zamarripa‑Colmenero, Alfredo, additional, Montes‑García, Noe, additional, Nuñez‑Colín, Carlos, additional, Solis‑Bonilla, Jose Luis, additional, Aguilar‑Rangel, María Rocío, additional, and Prom, Louis, additional
- Published
- 2012
- Full Text
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38. Bacterial resistance to cationic antimicrobial peptides
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Anaya-López, José Luis, primary, López-Meza, Joel Edmundo, additional, and Ochoa-Zarzosa, Alejandra, additional
- Published
- 2012
- Full Text
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39. Caracterización Genética de Spodoptera frugiperda (Lepidoptera: Noctuidae) en México Usando Marcadores AFLP.
- Author
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Pecina-Quintero, Víctor, Anaya-López, José Luis, Cortez-Mondaca, Edgardo, Nuñez-Colín, Carlos Alberto, Herrera-Corredor, Carlos, Montes-García, No, Jimenez-Becerril, Maria Fernanda, and Gámez-Vazquez, Alfredo Josu
- Abstract
En el presente estudio se caracterizó genéticamente al gusano cogollero colectado en 10 estados de México, mediante marcadores AFLP (Polimorfismo en la Longitud del Fragmento Amplificado). Se detectaron altos niveles de polimorfismo (98%), además el análisis de las relaciones genéticas y el índice de diversidad de los datos moleculares indicaron amplia variabilidad genética de esta especie. Se formaron dos grupos de genotipos de S. frugiperda en el dendrograma de las relaciones genéticas, confirmando la presencia de dos razas que podrían haber desarrollado diferencias biológicas y fisiológicas. No se observó la agrupación por origen geográfico, por lo que se concluye que la variación genética identificada se asocia más con la presencia de las razas maíz y arroz, que con el origen geográfico de los especímenes. La combinación E-ACT+M-CAC fue la más eficiente para estimar las diferencias entre especímenes y separar ambos grupos de genotipos de S. frugiperda, ya que tuvo los valores de MI (80.52), RP (112.27), y PIC (0.297) más altos, así como una correlación alta y significativa entre PIC y MI (r2 = 0.91, p < 0.05), y entre RP y MI (r2 = 0.99, p < 0.05). In the present study, fall armyworm collected in 10 states of Mexico, was characterized genetically, using AFLP markers (Amplified Fragment Length Polymorphism). High levels of polymorphism (98%) were detected and the analysis of genetic relationships and diversity index of molecular data showed wide genetic variability of this species. Two groups were formed of the genotype S. frugiperda in the dendrogram of genetic relationships, confirming the presence of two strains which may have developed biological and physiological differences. No grouping by geographical origin was observed, so it is concluded that the identified genetic variation is associated more with the presence of corn and rice strains than the geographical origin of the specimens. The E-ACT+M-CAC combination was the most efficient to estimate the differences between specimens and separate the two groups of genotypes of S. frugiperda, because it had the highest MI (80.52), RP (112.27), and PIC (0.297) values, as well as a high and significant correlation between PIC and MI (r2 = 0.91, p < 0.05) and between RP and MI (r2 = 0.99, p < 0.05). [ABSTRACT FROM AUTHOR]
- Published
- 2015
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40. Characterization of Resistance to Pepper Huasteco Geminivirus in Chili Peppers from Yucatán, México
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Godínez-Hernández, Yolanda, primary, Anaya-López, José Luis, additional, Díaz-Plaza, Raúl, additional, González-Chavira, Mario, additional, Torres-Pacheco, Irineo, additional, Rivera-Bustamante, Rafael F., additional, and Guevara-González, Ramón G., additional
- Published
- 2001
- Full Text
- View/download PDF
41. Bacterial resistance to cationic antimicrobial peptides.
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Anaya-López, José Luis, López-Meza, Joel Edmundo, and Ochoa-Zarzosa, Alejandra
- Abstract
Naturally occurring cationic antimicrobial peptides (CAMPs) have been considered as promising candidates to treat infections caused by pathogenic bacteria to animals and humans. This assumption is based on their mechanism of action, which is mainly performed through electrostatic membrane interactions. Unfortunately, the rise in the reports that describe bacterial resistance to CAMPs has redefined their role as therapeutic agents. In this review, we describe the state of the art of the most common resistance mechanisms developed by bacteria to CAMPs, making special emphasis on resistance selection. Considering most of the resistance mechanisms here reviewed, the emergence of resistance is unlikely in the short term, however we also described evidences that show the evolution of resistance to CAMPs, reevaluating their use as good antibacterial agents. Finally, the knowledge related to the description of CAMP resistance mechanisms may provide useful information for improving strategies to control infections. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
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