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3. The legal and ethical framework governing body donation in Europe – 2nd update on current practice

Author

Anatomie, Infection & Immunity, Brenner, Erich, Bleys, Ronald L.A.W., de Caro, Raffaele, Catereniuc, Ilia, Chirculescu, Andy R.M., Destrieux, Christophe, Eppler, Elisabeth, Filgueira, Luis, Kachlik, David, Kiss, Péter, Lee, Clive, Matveeva, Niki, Natsis, Konstantinos, Pais, Diogo, Paulsen, Friedrich, Piagkou, Maria, Quondamatteo, Fabio, Reglődi, Dóra, Şendemir, Erdoğan, Tranum-Jensen, Jørgen, Tutkuviene, Janina, Vázquez Osorio, María Teresa, Anatomie, Infection & Immunity, Brenner, Erich, Bleys, Ronald L.A.W., de Caro, Raffaele, Catereniuc, Ilia, Chirculescu, Andy R.M., Destrieux, Christophe, Eppler, Elisabeth, Filgueira, Luis, Kachlik, David, Kiss, Péter, Lee, Clive, Matveeva, Niki, Natsis, Konstantinos, Pais, Diogo, Paulsen, Friedrich, Piagkou, Maria, Quondamatteo, Fabio, Reglődi, Dóra, Şendemir, Erdoğan, Tranum-Jensen, Jørgen, Tutkuviene, Janina, and Vázquez Osorio, María Teresa

Published
2024

4. The masseteric nerve for facial reanimation: Macroscopic and histomorphometric characteristics in 106 human cadavers and comparison of axonal ratio with recipient nerves

Author

Anatomie, Infection & Immunity, Kehrer, Andreas, Engelmann, Simon, Knoedler, Leonard, Klein, Silvan M., Anker, Alexandra M., Heidekrueger, Paul, Tamm, Ernst, Bleys, Ronald LAW, Prantl, Lukas, Ruewe, Marc, Anatomie, Infection & Immunity, Kehrer, Andreas, Engelmann, Simon, Knoedler, Leonard, Klein, Silvan M., Anker, Alexandra M., Heidekrueger, Paul, Tamm, Ernst, Bleys, Ronald LAW, Prantl, Lukas, and Ruewe, Marc

Published
2024

5. Metabolic pathways in immune senescence and inflammaging: Novel therapeutic strategy for chronic inflammatory lung diseases. An EAACI position paper from the Task Force for Immunopharmacology

Author

CTI Research, MS Dermatologie/Allergologie, Infection & Immunity, Anatomie, Roth-Walter, F, Adcock, I M, Benito-Villalvilla, C, Bianchini, R, Bjermer, L, Caramori, G, Cari, L, Chung, K F, Diamant, Z, Eguiluz-Gracia, I, Knol, E F, Jesenak, M, Levi-Schaffer, F, Nocentini, G, O'Mahony, L, Palomares, O, Redegeld, F, Sokolowska, M, Van Esch, B C A M, Stellato, C, CTI Research, MS Dermatologie/Allergologie, Infection & Immunity, Anatomie, Roth-Walter, F, Adcock, I M, Benito-Villalvilla, C, Bianchini, R, Bjermer, L, Caramori, G, Cari, L, Chung, K F, Diamant, Z, Eguiluz-Gracia, I, Knol, E F, Jesenak, M, Levi-Schaffer, F, Nocentini, G, O'Mahony, L, Palomares, O, Redegeld, F, Sokolowska, M, Van Esch, B C A M, and Stellato, C

Published
2024

6. Determinants of Physical Activity among Patients with Colorectal Cancer: From Diagnosis to 5 Years after Diagnosis

Author

Epi Kanker Team B, Cancer, Biostatistiek Onderwijs, Apotheek Bereidingen, Anatomie, MS CGO, MS Hematologie, MS Medische Oncologie, Epi Kanker, JC onderzoeksprogramma Cancer, Smit, Karel C, Derksen, Jeroen W G, Stellato, Rebecca K, van Lanen, Anne-Sophie, Wesselink, Evertine, Belt, Eric J Th, Cloos-van Balen, Marissa, Coene, Peter Paul L O, Dekker, Jan Willem T, de Groot, Jan Willem, Haringhuizen, Annebeth W, van Halteren, Henk K, van Heek, Tjarda T, Helgason, Helgi H, Hendriks, Mathijs P, de Hingh, Ignace H J T, Hoekstra, Ronald, Houtsma, Danny, Janssen, Johan J B, Kok, Niels, Konsten, Joop L M, Los, Maartje, Meijerink, Martijn R, Mekenkamp, Leonie J M, Peeters, Koen C M J, Polée, Marco B, Rietbroek, Ron C, Schiphorst, Anandi H W, Schrauwen, Ruud W M, Schreinemakers, Jennifer, Sie, Mark P S, Simkens, Lieke, Sonneveld, Eric J A, Terheggen, Frederiek, Valkenburg-van Iersel, Liselot, Vles, Wouter J, Wasowicz-Kemps, Daria K, de Wilt, Johannes H W, Kok, Dieuwertje E, Winkels, Renate M, Kampman, Ellen, van Duijnhoven, Fränzel J B, Koopman, Miriam, May, Anne M, Epi Kanker Team B, Cancer, Biostatistiek Onderwijs, Apotheek Bereidingen, Anatomie, MS CGO, MS Hematologie, MS Medische Oncologie, Epi Kanker, JC onderzoeksprogramma Cancer, Smit, Karel C, Derksen, Jeroen W G, Stellato, Rebecca K, van Lanen, Anne-Sophie, Wesselink, Evertine, Belt, Eric J Th, Cloos-van Balen, Marissa, Coene, Peter Paul L O, Dekker, Jan Willem T, de Groot, Jan Willem, Haringhuizen, Annebeth W, van Halteren, Henk K, van Heek, Tjarda T, Helgason, Helgi H, Hendriks, Mathijs P, de Hingh, Ignace H J T, Hoekstra, Ronald, Houtsma, Danny, Janssen, Johan J B, Kok, Niels, Konsten, Joop L M, Los, Maartje, Meijerink, Martijn R, Mekenkamp, Leonie J M, Peeters, Koen C M J, Polée, Marco B, Rietbroek, Ron C, Schiphorst, Anandi H W, Schrauwen, Ruud W M, Schreinemakers, Jennifer, Sie, Mark P S, Simkens, Lieke, Sonneveld, Eric J A, Terheggen, Frederiek, Valkenburg-van Iersel, Liselot, Vles, Wouter J, Wasowicz-Kemps, Daria K, de Wilt, Johannes H W, Kok, Dieuwertje E, Winkels, Renate M, Kampman, Ellen, van Duijnhoven, Fränzel J B, Koopman, Miriam, and May, Anne M

Published
2024

7. The Ability to Sustain Facial Expressions

Author

Zorgeenheid MKA/BT Medisch, MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, Schutte, Hilde, Bielevelt, Freek, Emohamadian, Hafsa, Muradin, Marvick S.M., Bleys, Ronald L.A.W., Rosenberg, Antoine J.W.P., Zorgeenheid MKA/BT Medisch, MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, Schutte, Hilde, Bielevelt, Freek, Emohamadian, Hafsa, Muradin, Marvick S.M., Bleys, Ronald L.A.W., and Rosenberg, Antoine J.W.P.

Published
2024

8. New method for analysing spatial relationships of facial muscles on MRI: a pilot study

Author

Zorgeenheid MKA/BT Medisch, MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, Schutte, H., Bielevelt, F., Muradin, M. S.M., Bleys, R. L.A.W., Rosenberg, A. J.W.P., Zorgeenheid MKA/BT Medisch, MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, Schutte, H., Bielevelt, F., Muradin, M. S.M., Bleys, R. L.A.W., and Rosenberg, A. J.W.P.

Published
2024

9. Silencing XIST on the future active X: Searching human and bovine preimplantation embryos for the repressor

Author

FAH klinische reproductie, Anatomie en fysiologie, Voortplanting paard, dES/dFAH FR, CS_Cancer, Locomotion - Integrating Sciences, Aksit, Melis A, Yu, Bo, Roelen, Bernard A J, Migeon, Barbara R, FAH klinische reproductie, Anatomie en fysiologie, Voortplanting paard, dES/dFAH FR, CS_Cancer, Locomotion - Integrating Sciences, Aksit, Melis A, Yu, Bo, Roelen, Bernard A J, and Migeon, Barbara R

Published
2024

10. Distinct cardiotoxic effects by venoms of a spitting cobra (Naja pallida) and a rattlesnake (Crotalus atrox) revealed using an ex vivo Langendorff heart model

Author

Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Vlasblom, Ronald, van Thiel, Jory, Bittenbinder, Mátyás A, van Rhijn, Jon-Ruben, Drost, Rinske, Muis, Lotte, Slagboom, Julien, Salvatori, Daniela, Kool, Jeroen, Veldman, Robert Jan, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Vlasblom, Ronald, van Thiel, Jory, Bittenbinder, Mátyás A, van Rhijn, Jon-Ruben, Drost, Rinske, Muis, Lotte, Slagboom, Julien, Salvatori, Daniela, Kool, Jeroen, and Veldman, Robert Jan

Published
2024

11. Surgical Technique of the 3-Dimensional-printed Personalized Hip Implant for the Treatment of Canine Hip Dysplasia

Author

Chirurgie, CS_Locomotion, Anatomie en fysiologie, CS_Cancer, Locomotion - Integrating Sciences, Locomotion - Surgery (CA), Kwananocha, Irin, Verseijden, Femke, Kamali, Seyed A., Magré, Joëll, Willemsen, Koen, Schouten, Jacobine C.M., Salvatori, Daniela, Tryfonidou, Marianna A., Meij, Björn P., Chirurgie, CS_Locomotion, Anatomie en fysiologie, CS_Cancer, Locomotion - Integrating Sciences, Locomotion - Surgery (CA), Kwananocha, Irin, Verseijden, Femke, Kamali, Seyed A., Magré, Joëll, Willemsen, Koen, Schouten, Jacobine C.M., Salvatori, Daniela, Tryfonidou, Marianna A., and Meij, Björn P.

Published
2024

12. Trabeculations of the porcine and human cardiac ventricles are different in number but similar in total volume

Author

Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Jensen, Bjarke, Salvatori, Daniela, Schouten, Jacobine, Meijborg, Veronique M F, Lauridsen, Henrik, Agger, Peter, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Jensen, Bjarke, Salvatori, Daniela, Schouten, Jacobine, Meijborg, Veronique M F, Lauridsen, Henrik, and Agger, Peter

Published
2024

13. The apron of the greater omentum of gastric cancer patients contains various lymphoid structures including lymph nodes

Author

Anatomie assistenten, MCS-team, Anatomie, Infection & Immunity, Pathologie Pathologen staf, Cancer, MS CGO, Schurink, B., Cleypool, C. G.J., Brosens, L. A.A., Ruurda, J. P., Mackaaij, Claire, Roeling, T. A.P., van Hillegersberg, R., Bleys, R. L.A.W., Anatomie assistenten, MCS-team, Anatomie, Infection & Immunity, Pathologie Pathologen staf, Cancer, MS CGO, Schurink, B., Cleypool, C. G.J., Brosens, L. A.A., Ruurda, J. P., Mackaaij, Claire, Roeling, T. A.P., van Hillegersberg, R., and Bleys, R. L.A.W.

Published
2023

15. Challenging Future Generations: A Qualitative Study of Students’ Attitudes toward the Transition to Animal-Free Innovations in Education and Research

Author

Andreoli, Lara, Vlasblom, Ronald, Drost, Rinske, Meijboom, Franck L.B., Salvatori, Daniela, Dep Population Health Sciences, AISS Sustainable Animal Stewardship, LS Wijsgerige Ethiek, OFR - Ethics Institute, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Dep Population Health Sciences, AISS Sustainable Animal Stewardship, LS Wijsgerige Ethiek, OFR - Ethics Institute, Anatomie en fysiologie, CS_Locomotion, and CS_Cancer

Subjects
animal testing, General Veterinary, translational research, animal ethics, non-animal methods, Animal Science and Zoology, challenge-based learning, veterinary(all), animal-free innovations
Abstract

In 2016, the Dutch government declared its commitment to phasing out animal experiments by 2025. Although a high number of animal experiments are still performed and the 2025 target will not be met, the commitment remains. Efforts are being made to identify levers that might foster the transition to animal-free science. Education has been found to play a key role in the future of animal-free science and young generations are increasingly seen as key stakeholders. However, their attitudes toward the transition to animal-free innovations have not been investigated. The present article focuses on the values and beliefs held by students, who in 2022, participated in the course ‘Replacing Animal Testing’ (RAT) Challenge, organized by a consortium of Dutch universities. Contextually, students’ motivations to follow the course were investigated. The research was based on a qualitative study, including semi-structured interviews and a literature review. Our analysis of the findings revealed that students feel aligned with the social, ethical, and scientific reasons that support the transition to animal-free innovations. Moreover, the participants identified a series of regulatory, educational, cultural, and political obstacles to the transition that align with those identified in recent literature. From the discussion of these findings, we extrapolated six fundamental challenges that need to be addressed to foster the transition to animal-free science in an acceptable and responsible way.

Published
2023

16. Teratoma Assay for Testing Pluripotency and Malignancy of Stem Cells: Insufficient Reporting and Uptake of Animal-Free Methods-A Systematic Review

Author

Montilla-Rojo, Joaquin, Bialecka, Monika, Wever, Kimberley E, Mummery, Christine L, Looijenga, Leendert H J, Roelen, Bernard A J, Salvatori, Daniela C F, Anatomie en fysiologie, CS_Cancer, Voortplanting paard, CS_Locomotion, Anatomie en fysiologie, CS_Cancer, Voortplanting paard, and CS_Locomotion

Subjects
Teratoma/pathology, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], Organic Chemistry, Embryonic Stem Cells/metabolism, Cell Differentiation, General Medicine, pluripotency, Catalysis, Cell Line, Injections, teratoma assay, Computer Science Applications, Inorganic Chemistry, Mice, All institutes and research themes of the Radboud University Medical Center, Pluripotent Stem Cells/metabolism, Humans, Animals, Physical and Theoretical Chemistry, Molecular Biology, Spectroscopy, hPSCs, malignancy
Abstract

Contains fulltext : 291249.pdf (Publisher’s version ) (Open Access) Pluripotency describes the ability of stem cells to differentiate into derivatives of the three germ layers. In reporting new human pluripotent stem cell lines, their clonal derivatives or the safety of differentiated derivatives for transplantation, assessment of pluripotency is essential. Historically, the ability to form teratomas in vivo containing different somatic cell types following injection into immunodeficient mice has been regarded as functional evidence of pluripotency. In addition, the teratomas formed can be analyzed for the presence of malignant cells. However, use of this assay has been subject to scrutiny for ethical reasons on animal use and due to the lack of standardization in how it is used, therefore questioning its accuracy. In vitro alternatives for assessing pluripotency have been developed such as ScoreCard and PluriTest. However, it is unknown whether this has resulted in reduced use of the teratoma assay. Here, we systematically reviewed how the teratoma assay was reported in publications between 1998 (when the first human embryonic stem cell line was described) and 2021. Our analysis of >400 publications showed that in contrast to expectations, reporting of the teratoma assay has not improved: methods are not yet standardized, and malignancy was examined in only a relatively small percentage of assays. In addition, its use has not decreased since the implementation of the ARRIVE guidelines on reduction of animal use (2010) or the introduction of ScoreCard (2015) and PluriTest (2011). The teratoma assay is still the preferred method to assess the presence of undifferentiated cells in a differentiated cell product for transplantation since the in vitro assays alone are not generally accepted by the regulatory authorities for safety assessment. This highlights the remaining need for an in vitro assay to test malignancy of stem cells.

Published
2023

17. The development of the amnion in mice and other amniotes

Author

Chuva de Sousa Lopes, Susana M, Roelen, Bernard A J, Lawson, Kirstie A, Zwijsen, An, Anatomie en fysiologie, Voortplanting paard, CS_Cancer, Anatomie en fysiologie, Voortplanting paard, and CS_Cancer

Subjects
clonal analysis, PLACENTATION, Agricultural and Biological Sciences(all), ORIGIN, Biochemistry, Genetics and Molecular Biology(all), Gastrulation, FATE, extraembryonic membranes, YOLK-SAC, MOUSE EMBRYOS, Biochemistry, General Biochemistry, Genetics and Molecular Biology, Mesoderm, Mice, DIFFERENTIATION, Animals, amniotes, GASTRULATION, amniochorionic fold, General Agricultural and Biological Sciences, RHYTHMIC CONTRACTIONS, STEM-CELLS, Genetics and Molecular Biology(all), amnion, mouse gastrulation
Abstract

The amnion is an extraembryonic tissue that evolutionarily allowed embryos of all amniotes to develop in a transient and local aquatic environment. Despite the importance of this tissue, very little is known about its formation and its molecular characteristics. In this review, we have compared the basic organization of the extraembryonic membranes in amniotes and describe the two types of amniogenesis, folding and cavitation. We then zoom in on the atypical development of the amnion in mice that occurs via the formation of a single posterior amniochorionic fold. Moreover, we consolidate lineage tracing data to better understand the spatial and temporal origin of the progenitors of amniotic ectoderm, and visualize the behaviour of their descendants in the extraembryonic–embryonic junctional region. This analysis provides new insight on amnion development and expansion. Finally, using an online-available dataset of single-cell transcriptomics during the gastrulation period in mice, we provide bioinformatic analysis of the molecular signature of amniotic ectoderm and amniotic mesoderm. The amnion is a tissue with unique biomechanical properties that deserves to be better understood. This article is part of the theme issue ‘Extraembryonic tissues: exploring concepts, definitions and functions across the animal kingdom’.

Published
2022

20. Correction of the pelvic incidence using a bilateral extending pelvic osteotomy: a proof of concept study

Author

Orthopaedie Onderzoek, Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, Ochtman, A. E.A., Bleys, R. L.A.W., Cunningham, J. E., Öner, F. C., van Gaalen, S. M., Orthopaedie Onderzoek, Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, Ochtman, A. E.A., Bleys, R. L.A.W., Cunningham, J. E., Öner, F. C., and van Gaalen, S. M.

Published
2023

21. Surgical techniques in restoration lumbar lordosis: a biomechanical human cadaveric study

Author

Orthopaedie Onderzoek, Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, Ochtman, A. E.A., Bisschop, A., Bleys, R. L.A.W., Öner, F. C., van Gaalen, S. M., Orthopaedie Onderzoek, Anatomie, Infection & Immunity, Regenerative Medicine and Stem Cells, MS Orthopaedie Algemeen, Ochtman, A. E.A., Bisschop, A., Bleys, R. L.A.W., Öner, F. C., and van Gaalen, S. M.

Published
2023

23. Epithelioid Fibrous Histiocytoma with CARS-ALK Fusion: First Case Report

Author

UCL - (SLuc) Service d'anatomie pathologique, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de dermatologie, UCL - (SLuc) Centre de génétique médicale UCL, Secco, Léo-Paul, Libbrecht, Louis, Seijnhaeve, Elsa, Eggers, Silke, Dekairelle, Anne-France, De Roo, An-Katrien, UCL - (SLuc) Service d'anatomie pathologique, UCL - SSS/IREC - Institut de recherche expérimentale et clinique, UCL - (SLuc) Service de dermatologie, UCL - (SLuc) Centre de génétique médicale UCL, Secco, Léo-Paul, Libbrecht, Louis, Seijnhaeve, Elsa, Eggers, Silke, Dekairelle, Anne-France, and De Roo, An-Katrien

Abstract

Epithelioid fibrous histiocytoma (EFH) is a type of uncommon skin tumor mostly harboring Anaplastic Lymphoma Kinase (ALK) gene rearrangement, with different fusion partners reported. Whether this tumor is a separate entity or has a relationship with conventional fibrous histiocytomas is still a matter of debate. Benign course is the rule after complete surgical excision. A rare subtype of EFH with fusiform cells has been described, with specific fusion partners. Inflammatory myofibroblastic tumor (IMT) is a type of soft tissue tumor rarer than EFH, and it can display distant metastases. Some cases of primary cutaneous IMT included two with Cysteinyl-tRNA Synthetase 1 (CARS)-ALK rearrangement. IMT can have the same fusion partners as EFH, such as DCTN1, TMP3 or EML4 genes. We report the case of a 42-year-old woman presenting EFH with fusiform morphology harboring CARS-ALK fusion and discuss similarities and differences with IMT.

Published
2023

24. Resection of a Giant Middel Mediastinum Paraganglioma by Clamshell Incision without Cardiopulmonary Bypass

Author

UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Piette, Wivine, Belhaj, Asmae, Stanciu pop, Claudia, Rondelet, Benoît, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, Piette, Wivine, Belhaj, Asmae, Stanciu pop, Claudia, and Rondelet, Benoît

Abstract

Paraganglioma is a tumor developed from paraganglia, which are groups of neuroendocrine cells located along the vascular and nerve axes of the head and neck and along the spine. These tumors are benign and still localized in more than 80% of cases. Middle mediastinal paragangliomas represent less than 1% of mediastinal tumors and grow from the superior or middle mediastinal autonomic ganglion; they mostly are non-functional and are found in older patients. The often large volume and complex location of these tumors make their surgical resection challenging forcing the surgical team to resort to cardiopulmonary bypass and practice transfection of the aortic artery. We successfully operated an 80-year-old patient by clamshell incision without bypass or arterial procedure.

Published
2023

25. Systemic Inflammation/Nutritional Status Scores Are Prognostic but Not Predictive in Metastatic Non-Small-Cell Lung Cancer Treated with First-Line Immune Checkpoint Inhibitors

Author

UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, UCL - (MGD) Unité de support scientifique, UCL - (MGD) Service de pneumologie, UCL - (MGD) Service de radiologie - résonance magnétique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de médecine nucléaire, UCL - (MGD) Service de radiothérapie, Mahiat, Cédric, Bihin, Benoît, Duplaquet, Fabrice, Stanciu Pop, Claudia, Dupont, Michaël, Vander Borght, Thierry, Rondelet, Benoît, Vanderick, Jean, Andre, Bénédicte, Pirard, Lionel, Ocak, Sebahat, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/PNEU - Pôle de Pneumologie, ORL et Dermatologie, UCL - (MGD) Unité de support scientifique, UCL - (MGD) Service de pneumologie, UCL - (MGD) Service de radiologie - résonance magnétique, UCL - (MGD) Service de chirurgie cardio-vasculaire et thoracique, UCL - (MGD) Service d'anatomie pathologique, UCL - (MGD) Service de médecine nucléaire, UCL - (MGD) Service de radiothérapie, Mahiat, Cédric, Bihin, Benoît, Duplaquet, Fabrice, Stanciu Pop, Claudia, Dupont, Michaël, Vander Borght, Thierry, Rondelet, Benoît, Vanderick, Jean, Andre, Bénédicte, Pirard, Lionel, and Ocak, Sebahat

Abstract

Biomarkers of systemic inflammation/nutritional status have been associated with outcomes in advanced-stage non-small-cell lung cancer (NSCLC) treated with immune checkpoint inhibitors (ICIs). However, most of them were not tested in cohorts of patients treated with ICIs in combination with chemotherapy (CT) (ICI + CT) or with CT alone, making it impossible to discriminate a predictive from a prognostic effect. We conducted a single-center retrospective study to search for associations between various baseline biomarkers/scores that reflected the systemic inflammation/nutritional status (Lung Immune Prognostic Index, Modified Lung Immune Prognostic Index, Scottish Inflammatory Prognostic Score, Advanced Lung Cancer Inflammation Index, EPSILoN, Prognostic Nutritional Index, Systemic Immune-Inflammation Index, Gustave Roussy Immune Score, Royal Marsden Hospital Prognostic Score, Lung Immuno-oncology Prognostic Score 3, Lung Immuno-oncology Prognostic Score 4, score published by Holtzman et al., and Glasgow Prognostic Score) and outcomes in metastatic NSCLC treated in a first-line setting either with ICI in monotherapy (cohort 1; n = 75), ICI + CT (cohort 2; n = 56), or CT alone (cohort 3; n = 221). In the three cohorts, the biomarkers/scores were moderately associated with overall survival (OS) and progression-free survival (PFS). Their prognostic performance was relatively poor, with a maximum c-index of 0.66. None of them was specific to ICIs and could help to choose the best treatment modality. The systemic inflammation/nutritional status, associated with outcomes independently of the treatment, is therefore prognostic but not predictive in metastatic NSCLC.

Published
2023

26. Challenging Future Generations: A Qualitative Study of Students’ Attitudes toward the Transition to Animal-Free Innovations in Education and Research

Author

Dep Population Health Sciences, AISS Sustainable Animal Stewardship, LS Wijsgerige Ethiek, OFR - Ethics Institute, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Andreoli, Lara, Vlasblom, Ronald, Drost, Rinske, Meijboom, Franck L.B., Salvatori, Daniela, Dep Population Health Sciences, AISS Sustainable Animal Stewardship, LS Wijsgerige Ethiek, OFR - Ethics Institute, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Andreoli, Lara, Vlasblom, Ronald, Drost, Rinske, Meijboom, Franck L.B., and Salvatori, Daniela

Published
2023

27. Teratoma Assay for Testing Pluripotency and Malignancy of Stem Cells: Insufficient Reporting and Uptake of Animal-Free Methods-A Systematic Review

Author

Anatomie en fysiologie, CS_Cancer, Voortplanting paard, CS_Locomotion, Montilla-Rojo, Joaquin, Bialecka, Monika, Wever, Kimberley E, Mummery, Christine L, Looijenga, Leendert H J, Roelen, Bernard A J, Salvatori, Daniela C F, Anatomie en fysiologie, CS_Cancer, Voortplanting paard, CS_Locomotion, Montilla-Rojo, Joaquin, Bialecka, Monika, Wever, Kimberley E, Mummery, Christine L, Looijenga, Leendert H J, Roelen, Bernard A J, and Salvatori, Daniela C F

Published
2023

29. Classification of Atretic Small Antral Follicles in the Human Ovary

Author

Anatomie en fysiologie, Voortplanting paard, CS_Cancer, Locomotion - Integrating Sciences, Wei, Fu, Fan, Xueying, Del Valle, Julieta S, Asseler, Joyce D, van der Meeren, Lotte E, Cheng, Hui, Roelen, Bernard A J, Louwe, Leoni A, Pilgram, Gonneke S K, van der Westerlaken, Lucette A J, van Mello, Norah M, Chuva de Sousa Lopes, Susana M, Anatomie en fysiologie, Voortplanting paard, CS_Cancer, Locomotion - Integrating Sciences, Wei, Fu, Fan, Xueying, Del Valle, Julieta S, Asseler, Joyce D, van der Meeren, Lotte E, Cheng, Hui, Roelen, Bernard A J, Louwe, Leoni A, Pilgram, Gonneke S K, van der Westerlaken, Lucette A J, van Mello, Norah M, and Chuva de Sousa Lopes, Susana M

Published
2023

30. Rotation of 3D Anatomy Models Is Associated with Underperformance of Students with Low Visual-Spatial Abilities: A Two-Center Randomized Crossover Trial

Author

Anatomie en fysiologie, FAH Evidence based Veterinary Medicine, CS_Cancer, CS_Locomotion, Locomotion - Integrating Sciences, van Leeuwen, Bo, Dollé, Anna, Vernooij, Hans, Hierck, Beerend, Salvatori, Daniela, Anatomie en fysiologie, FAH Evidence based Veterinary Medicine, CS_Cancer, CS_Locomotion, Locomotion - Integrating Sciences, van Leeuwen, Bo, Dollé, Anna, Vernooij, Hans, Hierck, Beerend, and Salvatori, Daniela

Published
2023

31. Preclinical model for lumbar interbody fusion in small ruminants: Rationale and guideline

Author

Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Duits, Anneli, Salvatori, Daniela, Schouten, Jacobine, van Urk, Paul, Gaalen, Steven van, Ottink, Karsten, Öner, Cuhmur, Kruyt, Moyo, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Duits, Anneli, Salvatori, Daniela, Schouten, Jacobine, van Urk, Paul, Gaalen, Steven van, Ottink, Karsten, Öner, Cuhmur, and Kruyt, Moyo

Published
2023

32. Development and characterization of agonistic antibodies targeting the Ig-like 1 domain of MuSK

Author

Pathobiologie, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Lim, Jamie L, Augustinus, Roy, Plomp, Jaap J, Roya-Kouchaki, Kasra, Vergoossen, Dana L E, Fillié-Grijpma, Yvonne, Struijk, Josephine, Thomas, Rachel, Salvatori, Daniela, Steyaert, Christophe, Blanchetot, Christophe, Vanhauwaert, Roeland, Silence, Karen, van der Maarel, Silvère M, Verschuuren, Jan J, Huijbers, Maartje G, Pathobiologie, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Locomotion - Integrating Sciences, Lim, Jamie L, Augustinus, Roy, Plomp, Jaap J, Roya-Kouchaki, Kasra, Vergoossen, Dana L E, Fillié-Grijpma, Yvonne, Struijk, Josephine, Thomas, Rachel, Salvatori, Daniela, Steyaert, Christophe, Blanchetot, Christophe, Vanhauwaert, Roeland, Silence, Karen, van der Maarel, Silvère M, Verschuuren, Jan J, and Huijbers, Maartje G

Published
2023

33. Co-constructive Veterinary Simulation: A Novel Approach to Enhancing Clinical Communication and Reflection Skills

Author

Interne geneeskunde GD, Anatomie en fysiologie, Faculteit Diergeneeskunde, Spruijt, Annemarie, Prins-Aardema, Cecil C, Antonio de Carvalho-Filho, Marco, Jaarsma, Debbie, Martin, Andrés, Interne geneeskunde GD, Anatomie en fysiologie, Faculteit Diergeneeskunde, Spruijt, Annemarie, Prins-Aardema, Cecil C, Antonio de Carvalho-Filho, Marco, Jaarsma, Debbie, and Martin, Andrés

Published
2023

34. Impact of Simulated Rotating Shift Work on Mammary Tumor Development in the p53R270H©/+WAPCre Mouse Model for Breast Cancer

Author

CS_Cancer, CS_Locomotion, Anatomie en fysiologie, Locomotion - Integrating Sciences, Streng, Astrid A, Van Dycke, Kirsten C G, van Oostrom, Conny T M, Salvatori, Daniela C F, Hulsegge, Gerben, Chaves, Inês, Roenneberg, Till, Zander, Serge A L, van Steeg, Harry, van der Horst, Gijsbertus T J, van Kerkhof, Linda W M, CS_Cancer, CS_Locomotion, Anatomie en fysiologie, Locomotion - Integrating Sciences, Streng, Astrid A, Van Dycke, Kirsten C G, van Oostrom, Conny T M, Salvatori, Daniela C F, Hulsegge, Gerben, Chaves, Inês, Roenneberg, Till, Zander, Serge A L, van Steeg, Harry, van der Horst, Gijsbertus T J, and van Kerkhof, Linda W M

Published
2023

35. 3D printed and punched porous surfaces of a non-resorbable, biphasic implant for the repair of osteochondral lesions improves repair tissue adherence and ingrowth

Author

Orthopaedie Onderzoek, Anatomie, ORT Research, Regenerative Medicine and Stem Cells, Fugazzola, Maria C., Golafshan, Nasim, van Aken, Joris A., Plomp, Saskia, De Grauw, Janny, van Buul, Ward, Hermsen, Gied, Weinans, Harrie, Castilho, Miguel, van Weeren, René, Malda, Jos, Orthopaedie Onderzoek, Anatomie, ORT Research, Regenerative Medicine and Stem Cells, Fugazzola, Maria C., Golafshan, Nasim, van Aken, Joris A., Plomp, Saskia, De Grauw, Janny, van Buul, Ward, Hermsen, Gied, Weinans, Harrie, Castilho, Miguel, van Weeren, René, and Malda, Jos

Published
2023

36. A novel anatomical description of the esophagus: the supracarinal mesoesophagus

Author

Anatomie, Infection & Immunity, MS CGO, Cancer, Cuesta, Miguel A, van Jaarsveld, Romy C, Mingol, Fernando, Bleys, Ronald L A W, van Hillegersberg, Richard, Padules, Carmen, Bruna, Marcos, Ruurda, Jelle P, Anatomie, Infection & Immunity, MS CGO, Cancer, Cuesta, Miguel A, van Jaarsveld, Romy C, Mingol, Fernando, Bleys, Ronald L A W, van Hillegersberg, Richard, Padules, Carmen, Bruna, Marcos, and Ruurda, Jelle P

Published
2023

37. The Average Facial Expressions: A Range of Motion Analysis for Different Sex and Age Groups

Author

MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Zorgeenheid MKA/BT Medisch, Infection & Immunity, Anatomie, Schutte, Hilde, Muradin, Marvick S.M., Bielevelt, Freek, Seubring, Karlien, Bleys, Ronald L.A.W., Rosenberg, Antoine J.W.P., MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Zorgeenheid MKA/BT Medisch, Infection & Immunity, Anatomie, Schutte, Hilde, Muradin, Marvick S.M., Bielevelt, Freek, Seubring, Karlien, Bleys, Ronald L.A.W., and Rosenberg, Antoine J.W.P.

Published
2023

38. The average three-dimensional face for different sex and age groups in a Dutch population

Author

MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Anatomie, Infection & Immunity, Zorgeenheid MKA/BT Medisch, Schutte, H., Muradin, M. S.M., Bielevelt, F., Janssen, N. G., Bleys, R. L.A.W., Rosenberg, A. J.W.P., MKA/BT Onderzoek, MS Mondziekten/Kaakchirurgie, Other research (not in main researchprogram), Anatomie, Infection & Immunity, Zorgeenheid MKA/BT Medisch, Schutte, H., Muradin, M. S.M., Bielevelt, F., Janssen, N. G., Bleys, R. L.A.W., and Rosenberg, A. J.W.P.

Published
2023

39. The surgical anatomy of a (robot-assisted) minimally invasive transcervical esophagectomy

Author

Cancer, MS CGO, Anatomie, Infection & Immunity, Filz von Reiterdank, I C L J, Defize, I L, de Groot, E M, Wedel, T, Grimminger, P P, Egberts, J H, Stein, H, Ruurda, J P, van Hillegersberg, R, Bleys, R L A W, Cancer, MS CGO, Anatomie, Infection & Immunity, Filz von Reiterdank, I C L J, Defize, I L, de Groot, E M, Wedel, T, Grimminger, P P, Egberts, J H, Stein, H, Ruurda, J P, van Hillegersberg, R, and Bleys, R L A W

Published
2023

40. Les journalistes minorisé·es face à l’accusation de wokisme

Author

Anatomie de l'anti-wokisme (11 décembre 2023: Université Saint-Louis, Bruxelles), Louazon, Elena, Petit, Clémence, Anatomie de l'anti-wokisme (11 décembre 2023: Université Saint-Louis, Bruxelles), Louazon, Elena, and Petit, Clémence

Abstract

info:eu-repo/semantics/nonPublished

Published
2023

41. Making It Real

Author

Martin, A, de Carvalho Filho, M.A., Jaarsma, Debbie, Duvivier, R, Anatomie en fysiologie, Faculteit Diergeneeskunde, Lifelong Learning, Education & Assessment Research Network (LEARN), Anatomie en fysiologie, and Faculteit Diergeneeskunde

Subjects
Medical education, Psychiatry, Medicine(all), PORTRAYAL, Reflective practice, medicine.medical_specialty, video-enhanced didactic, GUIDANCE, ILLNESS, SIMULATED PATIENTS, psychiatry, Education, Stigma, Psychiatric education, stigma, reflective practice, Video-enhanced didactic, medicine, Advances in Medical Education and Practice, Simulated patients, Psychology, medical education
Abstract

Andrés Martin,1– 3 Marco A de Carvalho Filho,3,4 Debbie Jaarsma,3,5 Robbert Duvivier3,6 1Child Study Center, Yale School of Medicine, New Haven, CT, USA; 2Simulated Participant Program, Teaching and Learning Center, Yale School of Medicine, New Haven, CT, USA; 3Center for Educational Development and Research in Health Sciences (CEDAR), Lifelong Learning, Education and Assessment Research Network (LEARN), University Medical Center Groningen, Groningen, the Netherlands; 4School of Medical Sciences, University of Minho, Braga, Portugal; 5Faculty of Veterinary Medicine, University of Utrecht, Utrecht, the Netherlands; 6Parnassia Psychiatric Institute, The Hague, the NetherlandsCorrespondence: Andrés MartinYale Child Study Center, 230 South Frontage Road, New Haven, CT, 06520-7900, USATel +1 203-494-2355Fax + 1 203-785-7400Email andres.martin@yale.eduObjective: Innovations in contemporary medical education could inform remedies to address enduring challenges such as the marginalization and stigmatization of psychiatry, of mental illnesses, and of those affected by them.Methods: In blending the works of Bleakley, Bligh, and Brown (2011) and of Kumagai and Naidu (2015), we developed an overarching heuristic with practical relevance and concrete applications to psychiatric education.Results: We identify three areas to enhance psychiatric education embedded into this blended framework: 1) Showing, or the more accurate depiction or imaging of mental illnesses and of psychiatric practice, as exemplified by the incorporation into didactic content of asynchronous video-based clinical materials produced with specific educational objectives in mind; 2) Sharing, or addressing the image problem of mental illnesses, of those living with or affected by them, and of psychiatry as a profession, as exemplified by psychiatrists embracing their role as experts by professional and personal experience when sharing their own journeys with mental illness, treatment, and recovery; and 3) Doing, or reimagining reflective psychiatric practice, as exemplified by the novel methodology of co-constructive patient simulation (CCPS), through which learners can engage in reflective practice and supervision in a participatory and democratic setting that does not privilege participants’ hierarchical standing.Conclusion: The blended model and the sample applications we describe offer a range of teaching, learning, and professional development opportunities, should psychiatric educators choose to pursue them and reap their promise.Keywords: medical education, psychiatry, reflective practice, simulated patients, stigma, video-enhanced didactic

Published
2021

42. Axin2/Conductin is required for normal hematopoiesis and T lymphopoiesis

Author

de Roo, Jolanda J D, Chhatta, Amiet, Garcia-Perez, Laura, Naber, Brigitta A E, Vloemans, Sandra A, Salvatori, Daniela C F, Pike-Overzet, Karin, Mikkers, Harald, Staal, Frank J T, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Anatomie en fysiologie, CS_Locomotion, and CS_Cancer

Subjects
Axin Protein/genetics, Biochemistry, Genetics and Molecular Biology(all), WNT- thymus-hematopoietic stem cell, Lymphopoiesis, Lymphopoiesis/genetics, Cell Differentiation, General Medicine, Hematopoietic Stem Cells, Biochemistry, Hematopoiesis, Hematopoiesis/genetics, Mice, Axin Protein, Animals, Wnt Signaling Pathway, Genetics and Molecular Biology(all)
Abstract

The development of T lymphocytes in the thymus and their stem cell precursors in the bone marrow is controlled by Wnt signaling in strictly regulated, cell-type specific dosages. In this study, we investigated levels of canonical Wnt signaling during hematopoiesis and T cell development within the Axin2-mTurquoise2 reporter. We demonstrate active Wnt signaling in hematopoietic stem cells (HSCs) and early thymocytes, but also in more mature thymic subsets and peripheral T lymphocytes. Thymic epithelial cells displayed particularly high Wnt signaling, suggesting an interesting crosstalk between thymocytes and thymic epithelial cells (TECs). Additionally, reporter mice allowed us to investigate the loss of Axin2 function, demonstrating decreased HSC repopulation upon transplantation and the partial arrest of early thymocyte development in Axin2Tg/Tg full mutant mice. Mechanistically, loss of Axin2 leads to supraphysiological Wnt levels that disrupt HSC differentiation and thymocyte development.

Published
2022

43. Prevalence of Gastric Inlet Patches of the Cervical Esophagus.

Author

Institut für Allgemeine Pathologie und Pathologische Anatomie der TU München, Klinik für Kinder und Jugendmedizin, Klinikum Dritter Orden; München, Klinik für Kinder und Jugendmedizin, Universitätsklinikum Ulm, Kinderklinik im Dr. von Haunerschen Kinderspital, Universität München, and Monther Bajbouj, MD

Published
2010

44. The centriolar satellite protein Cfap53 facilitates formation of the zygotic microtubule organizing center in the zebrafish embryo

Author

Willekers, Sven, Tessadori, Federico, van der Vaart, Babet, Henning, Heiko H., Stucchi, Riccardo, Altelaar, Maarten, Roelen, Bernard A.J., Akhmanova, Anna, Bakkers, Jeroen, Sub Cell Biology, Voortplanting paard, Afd Biomol.Mass Spect. and Proteomics, Anatomie en fysiologie, Celbiologie, Al het onderzoek van de faculteit Diergeneeskunde, CS_Cancer, Hubrecht Institute for Developmental Biology and Stem Cell Research, Sub Cell Biology, Voortplanting paard, Afd Biomol.Mass Spect. and Proteomics, Anatomie en fysiologie, Celbiologie, Al het onderzoek van de faculteit Diergeneeskunde, and CS_Cancer

Subjects
Centrosome, Male, Cell division, Ccdc11, Semen/metabolism, Tubulin/metabolism, Maternal, Microtubule-Organizing Center/metabolism, Cfap53, Paternal, Centrioles/metabolism, Centrosome/metabolism, MTOC, Semen, Tubulin, Zebrafish/metabolism, Centriolar satellites, Animals, Molecular Biology, Microtubule-Organizing Center, Zebrafish, Centrioles, Developmental Biology
Abstract

In embryos of most animal species, the zygotic centrosome is assembled by the centriole derived from the sperm cell and pericentriolar proteins present in the oocyte. This zygotic centrosome acts as a microtubule organizing center (MTOC) to assemble the sperm aster and mitotic spindle. As MTOC formation has been studied mainly in adult cells, very little is known about the formation of the zygotic MTOC. Here, we show that zebrafish (Danio rerio) embryos lacking either maternal or paternal Cfap53, a centriolar satellite protein, arrest during the first cell cycle. Although Cfap53 is dispensable for sperm aster function, it aids proper formation of the mitotic spindle. During cell division, Cfap53 colocalizes with γ-tubulin and with other centrosomal and centriolar satellite proteins at the MTOC. Furthermore, we find that γ-tubulin localization at the MTOC is impaired in the absence of Cfap53. Based on these results, we propose a model in which Cfap53 deposited in the oocyte and the sperm participates in the organization of the zygotic MTOC to allow mitotic spindle formation.

Published
2022

45. Reporting of anaesthesia and pain management in preclinical large animal models of articular cartilage repair - A long way to go

Author

Fugazzola, Maria C, Wever, Kimberley E, van de Lest, Chris, de Grauw, Janny, Salvatori, Daniela, CS_Locomotion, Equine Musculoskeletal Biology, Veterinaire biochemie, Anesthesiologie, Anatomie en fysiologie, CS_Cancer, CS_Locomotion, Equine Musculoskeletal Biology, Veterinaire biochemie, Anesthesiologie, Anatomie en fysiologie, and CS_Cancer

Subjects
Cartilage repair, Reporting quality, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], ARRIVE guidelines, Vascular damage Radboud Institute for Health Sciences [Radboudumc 16], Large animal models, General Medicine, Pain management
Abstract

Contains fulltext : 286723.pdf (Publisher’s version ) (Open Access) Animal models continue to be used to investigate cartilage repair strategies. Adequate anaesthesia and pain management are essential in order to guarantee acceptable animal welfare as well as reproducible experimental results. This systematic review evaluates reporting of anaesthesia and pain management in surgical large animal models (horse, pig, dog, goat and sheep) of (osteo)chondral repair. Manuscripts published between 2015 and 2020 were included after a comprehensive search strategy. Data were evaluated using descriptive statistics and qualitative review. Out of 223 eligible studies, 220 studies contained incomplete information on anaesthetic and pain management. Pre-, intra- and post-operative analgesia were not mentioned in 68%, 94%, and 64% of manuscripts respectively. A total of 176 studies reported that animals underwent general anaesthesia during surgery. Surprisingly, 30% of these articles did not provide any detail on anaesthetic management, while 37% reported using inhalant, hypnotic or sedative drugs only, without mention of analgesics. Pain monitoring was not reported in 87% of manuscripts. The vast majority of preclinical large animal studies on cartilage repair did not meet veterinary clinical standards for anaesthesia and analgesia, and failed to report according to the ARRIVE international guidelines. In light of serious welfare, ethical and translational validity concerns, improvement is urgently needed.

Published
2022

46. Consequences of excessive glucosylsphingosine in glucocerebrosidase-deficient zebrafish

Author

Lelieveld, Lindsey T, Gerhardt, Sophie, Maas, Saskia, Zwiers, Kimberley C, de Wit, Claire, Beijk, Ernst H, Ferraz, Maria J, Artola, Marta, Meijer, Annemarie H, Tudorache, Christian, Salvatori, Daniela, Boot, Rolf G, Aerts, Johannes M F G, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Human Genetics, Amsterdam Neuroscience - Cellular & Molecular Mechanisms, Amsterdam Neuroscience - Complex Trait Genetics, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, and Amsterdam Reproduction & Development (AR&D)

Subjects
dopaminergic neurons, sphingolipids, acid ceramidase, Psychosine, Gaucher disease, Cell Biology, Zebrafish Proteins, Glucosylceramides, zebrafish, Biochemistry, Endocrinology, glucosylceramide, lysosphingolipids, lipid metabolism, Animals, Glucosylceramidase, Humans, Supplementary key words Gaucher disease
Abstract

In Gaucher disease (GD), the deficiency of glucocerebrosidase causes lysosomal accumulation of glucosylceramide (GlcCer), which is partly converted by acid ceramidase to glucosylsphingosine (GlcSph) in the lysosome. Chronically elevated blood and tissue GlcSph is thought to contribute to symptoms in GD patients as well as to increased risk for Parkinson's disease. On the other hand, formation of GlcSph may be beneficial since the water soluble sphingoid base is excreted via urine and bile. To study the role of excessive GlcSph formation during glucocerebrosidase deficiency, we studied zebrafish that have two orthologs of acid ceramidase, Asah1a and Asah1b. Only the latter is involved in the formation of GlcSph in glucocerebrosidase-deficient zebrafish as revealed by knockouts of Asah1a or Asah1b with glucocerebrosidase deficiency (either pharmacologically induced or genetic). Comparison of zebrafish with excessive GlcSph (gba1-/- fish) and without GlcSph (gba1-/-:asah1b-/- fish) allowed us to study the consequences of chronic high levels of GlcSph. Prevention of excessive GlcSph in gba1-/-:asah1b-/- fish did not restrict storage cells, GlcCer accumulation, or neuroinflammation. However, GD fish lacking excessive GlcSph show an ameliorated course of disease reflected by significantly increased lifespan, delayed locomotor abnormality, and delayed development of an abnormal curved back posture. The loss of tyrosine hydroxylase 1 (th1) mRNA, a marker of dopaminergic neurons, is slowed down in brain of GD fish lacking excessive GlcSph. In conclusion, in the zebrafish GD model, excess GlcSph has little impact on (neuro)inflammation or the presence of GlcCer-laden macrophages but rather seems harmful to th1-positive dopaminergic neurons.

Published
2022

48. PRMT3 inhibitor SGC707 reduces triglyceride levels and induces pruritus in Western-type diet-fed LDL receptor knockout mice

Author

de Jong, Laura M, Zhang, Zhengzheng, den Hartog, Yvette, Sijsenaar, Timothy J P, Martins Cardoso, Renata, Manson, Martijn L, Hankemeier, Thomas, Lindenburg, Peter W, Salvatori, Daniela C F, Van Eck, Miranda, Hoekstra, Menno, Anatomie en fysiologie, CS_Locomotion, CS_Cancer, Anatomie en fysiologie, CS_Locomotion, and CS_Cancer

Subjects
Male, Protein-Arginine N-Methyltransferases, LDL/deficiency, Science, Knockout, Inbred C57BL, Receptors, LDL/deficiency, Article, Fatty Liver/drug therapy, Receptors, G-Protein-Coupled, Diet, Western/adverse effects, Mice, Liver/drug effects, Receptors, Animals, Humans, Protein-Arginine N-Methyltransferases/antagonists & inhibitors, General, Triglycerides, G-Protein-Coupled/genetics, Mice, Knockout, Western/adverse effects, Isoquinolines/adverse effects, Multidisciplinary, Pruritus, Isoquinolines, Lipids, Diet, Fatty Liver, Mice, Inbred C57BL, Triglycerides/blood, Metabolism, Pruritus/etiology, Liver, Receptors, LDL, Diet, Western, Medicine, Receptors, G-Protein-Coupled/genetics
Abstract

Protein arginine methyltransferase 3 (PRMT3) is a co-activator of liver X receptor capable of selectively modulating hepatic triglyceride synthesis. Here we investigated whether pharmacological PRMT3 inhibition can diminish the hepatic steatosis extent and lower plasma lipid levels and atherosclerosis susceptibility. Hereto, male hyperlipidemic low-density lipoprotein receptor knockout mice were fed an atherogenic Western-type diet and injected 3 times per week intraperitoneally with PRMT3 inhibitor SGC707 or solvent control. Three weeks into the study, SGC707-treated mice developed severe pruritus and scratching-associated skin lesions, leading to early study termination. SGC707-treated mice exhibited 50% lower liver triglyceride stores as well as 32% lower plasma triglyceride levels. Atherosclerotic lesions were virtually absent in all experimental mice. Plasma metabolite analysis revealed that levels of taurine-conjugated bile acids were ~ threefold increased (P

Published
2022

49. Comparing Blind and Ultrasound-Guided Retrobulbar Nerve Blocks in Equine Cadavers: The Training Effect

Author

Hermans, Hanneke, Lloyd-Edwards, Ralph A, Ferrão-van Sommeren, Aukje J H, Tersmette, Anne A, Schouten, Jacobine C M, Serra Bragança, Filipe M, van Loon, Johannes P A M, Heelkunde, dES AVR, Anatomie en fysiologie, Equine Musculoskeletal Biology, dES RMSC, CS_Welfare & emerging diseases, Heelkunde, dES AVR, Anatomie en fysiologie, Equine Musculoskeletal Biology, dES RMSC, and CS_Welfare & emerging diseases

Subjects
General Veterinary, Horse head, Veterinary medicine, local, anesthetic, horse head, welfare, ophthalmic, pain management, Anesthetic, Welfare, veterinary(all), Article, Ophthalmic, Pain management, QL1-991, Local, SF600-1100, Animal Science and Zoology, Zoology
Abstract

Simple Summary Standing ophthalmic surgeries have become more and more common in horses. For these standing surgeries, the blind retrobulbar block is often used for anesthesia and akinesia of the eye. However, placing a retrobulbar block using this blind technique can lead to complications, for example, penetration of the globe, nerve injury or chemosis due to spreading of the local anesthetic in the region of the globe. For this reason, it might be better to perform the retrobulbar block using guidance by ultrasound. Ultrasound-guided retrobulbar block has only been described twice in the literature using equine cadavers. Comparison of the ultrasound-guided peribulbar technique to the blind technique has only been carried out once. Furthermore, the learning curve of ultrasound-guided retrobulbar nerve block placement has not been evaluated. Our study aimed to compare the blind and ultrasound-guided approaches to retrobulbar block placement in horses and to evaluate the success and complication rates, analyzing the effect of training on ultrasound guidance. A trend towards a significant improvement in accuracy was seen for ultrasound guidance, and larger scale follow-up studies might show a significant training effect on the use of ultrasound in retrobulbar nerve block placement and that the use of ultrasound guidance could be promising. Abstract In standing ophthalmic surgery in horses, a retrobulbar nerve block (RNB) is often placed blindly for anesthesia and akinesia. The ultrasound (US)-guided RNB may have fewer complications, but the two techniques have only been compared once in equine cadavers. This study compares the techniques for success and complication rates and analyzes the effect of training on US guidance. Twenty-two equine cadavers were divided into three groups: blind RNBs were performed bilaterally in eight cadavers, US-guided RNBs were performed bilaterally in seven cadavers, and after US-guided training, blind RNBs were performed bilaterally in seven cadavers. All RNBs were performed by the same two inexperienced operators, and a combination of contrast medium (CM; 1.25 mL) and methylene blue dye (1.25 mL) were injected (2.5 mL total volume). Needle positioning in the periorbita and the distance of the CM to the optic foramen were assessed using computerized tomography (CT). Dye spreading was evaluated by dissection. In group 1, 37.5% of the injections were in the optimal central position in the periorbita; in group 2, 75% and in group 3, 71.4%. There was no significant difference between the groups regarding needle position (groups 1 and 2 p = 0.056; groups 1 and 3 p = 0.069, groups 2 and 3 p = 0.8). The mean CM distribution distance was not significantly different between all groups. Group 1 had 18.75% intraocular injections versus 0% in group 2 and 7.1% in group 3 (not significant). US guidance showed no significant increases in accuracy nor decreases in complications. However, the effects on accuracy showed a trend towards significant improvement, and larger scale follow-up studies might show significant training effects on US guidance.

Published
2022

50. Morphological intratumor heterogeneity in ductal carcinoma in situ of the breast.

Author

UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/GYNE - Pôle de Gynécologie, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (MGD) Service d'anatomie pathologique, UCL - (SLuc) Service d'anatomie pathologique, UCL - (SLuc) Service de gynécologie et d'andrologie, UCL - (SLuc) Unité d'oncologie médicale, UCL - (SLuc) Service de radiologie, UCL - (SLuc) Centre du cancer, Stanciu Pop, Claudia Maria, Nollevaux, Marie-Cécile, Berlière, Martine, Duhoux, François, Fellah, Latifa, Galant, Christine, Van Bockstal, Mieke, UCL - SSS/IREC/MORF - Pôle de Morphologie, UCL - SSS/IREC/MIRO - Pôle d'imagerie moléculaire, radiothérapie et oncologie, UCL - SSS/IREC/MONT - Pôle Mont Godinne, UCL - SSS/IREC/GYNE - Pôle de Gynécologie, UCL - SSS/IREC/SLUC - Pôle St.-Luc, UCL - (MGD) Service d'anatomie pathologique, UCL - (SLuc) Service d'anatomie pathologique, UCL - (SLuc) Service de gynécologie et d'andrologie, UCL - (SLuc) Unité d'oncologie médicale, UCL - (SLuc) Service de radiologie, UCL - (SLuc) Centre du cancer, Stanciu Pop, Claudia Maria, Nollevaux, Marie-Cécile, Berlière, Martine, Duhoux, François, Fellah, Latifa, Galant, Christine, and Van Bockstal, Mieke

Abstract

Ductal carcinoma in situ (DCIS) of the breast is a heterogeneous disease in terms of morphological characteristics, protein expression profiles, genetic abnormalities, and potential for progression. Molecular heterogeneity has been extensively studied in DCIS. Yet morphological heterogeneity remains relatively undefined. This study investigated morphological intratumor heterogeneity in a series of 51 large DCIS. Nuclear atypia, DCIS architecture, necrosis, calcifications, stromal architecture, and stromal inflammation were assessed in one biopsy slide and three representative slides from each corresponding resection. For each histopathological feature, a histo-score was determined per slide and compared between the biopsy and the resection, as well as within a single resection. Statistical analysis comprised of Friedman tests, post hoc Wilcoxon tests with Bonferroni corrections, Mann-Whitney U tests, and chi-square tests. Despite substantial morphological heterogeneity in around 50% of DCIS, the histopathological assessment of the biopsy did not statistically significantly differ from the resection. Morphological heterogeneity was not significantly associated with patient age, DCIS size, or type of surgery, except for a weak association between heterogeneous stromal inflammation and smaller DCIS size. At the group level, the degree of heterogeneity did not significantly affect the representativity of a biopsy. At the individual patient level, however, the presence of necrosis, intraductal calcifications, myxoid stromal changes, and high-grade nuclear atypia was underestimated in a minority of DCIS patients. This study confirms the presence of morphological heterogeneity in DCIS for all six evaluated histopathological features. This should be kept in mind when taking biopsy-based treatment decisions for DCIS patients.

Published
2021

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