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Dissertação de mestrado em Língua, Literatura e Cultura Inglesas, Os Estudos de Receção Clássica exploram como os materiais gregos e romanos foram "transmitidos, traduzidos, extraídos, interpretados, reescritos e representados" (Hardwick & Stray, 2008, p.1). Os estudos analisam a trajetória do material clássico ao longo dos anos, e investigam as sociedades em que os materiais são recebidos. Investigam igualmente as obras que foram censuradas ou esquecidas, e questionam a prevalência de determinadas obras e prospeções. Madeline Miller escreveu o célebre livro intitulado "O Canto de Aquiles", uma releitura da Ilíada através do ponto de vista de Pátroclo. A autora recorre ao material grego e romano para reescrever a história como um romance homossexual entre os heróis gregos. Quase uma década depois, o livro ganhou uma nova popularidade, graças à aplicação da rede social TikTok, especificamente a comunidade denominada "#BookTok”. Na presente dissertação, será considerado como a autora recebeu, no âmbito dos Estudos de Receção, o material grego e romano, a sua perspetiva enquanto mulher a reescrever os clássicos, como as personagens LGBTQ+ foram representadas, e como tal influenciou a respetiva receção do livro. Adicionalmente, será analisada a importância da representação LGBTQ+ em termos de conteúdo visual e literário, e o impacto atual das redes sociais na receção do livro, bem como na generalidade da indústria literária., Classical Reception Studies explore how the Greek and Roman materials have “been transmitted, translated, excerpted, interpreted, rewritten, re-imaged and represented” (Hardwick & Stray, 2008, p.1). It examines the trajectory of Classical material throughout the years, as well as investigates the societies in which the materials were received. This area of expertise also researches the works that have been silenced or forgotten, and questions the reason for the survival of some works and perspectives over others. Madeline Miller famously wrote The Song of Achilles, a reimagining of The Iliad through Patroclus’s point of view. The author uses Greek and Roman material to rewrite the story as a homosexual romance between the Greek heroes. Almost a decade later, the book gained traction as the social media app TikTok, specifically the #BookTok community, gave rise to the new popularity of the book. In this dissertation, we will consider how the author received the Greek and Roman material, her perspective as a woman rewriting the Classics, the way the LGBTQ+ characters were represented, and how it affected its reception. Furthermore, we will analyse the importance of LGBTQ+ representation in visual and literary content, and the current impact of social media in the reception of the book, as well as in the literary industry in general.

O cultivo controlado de macroalgas marinhas tem sido cada vez mais incentivado, utilizando métodos sustentáveis e em linha com as problemáticas de poluição atuais. Surge, neste sentido, a aquacultura multi-trófica integrada (IMTA), realizada de forma pioneira em Portugal pela ALGAplus, Lda. com diferentes espécies de algas, entre as quais a Ulva rigida, uma das macroalgas mais consumidas e utilizadas mundialmente. Deste modo, a empresa deve zelar pela entrega de um produto com o máximo de qualidade para o cliente, através de uma otimização contínua dos seus processos. Foram definidos como objetivos para esta dissertação inserida em estágio curricular na empresa ALGAplus, o estudo e melhoria de diferentes fases do processamento da Ulva sp. Houve a realização de estudos de cinética de secagem para otimizar a etapa de desidratação da macroalga e um estudo sobre a sua qualidade nutricional aquando da produção, relacionando condições de cultivo com a qualidade da biomassa obtida. Para este último, o objetivo final foi o de validação de uma escala que relacione a cor com a concentração de azoto total nas amostras, tornando-se uma ferramenta prática de avaliação da qualidade da biomassa para utilização rotineira na empresa. Foram possíveis observar variações significativas na desidratação entre as diferentes posições do túnel de secagem, sendo esta realizada de forma mais homogénea junto à porta de saída (Torre 9 a 12), tendo sido também evidenciada que a possível quantidade de biomassa mais indicada a colocar nos tabuleiros para secagem será entre 300 e 400 gramas. Deste modo, foi possível contribuir para uma sugestão de atualização das instruções de trabalho da empresa, de modo a aumentar a eficiência do processo de secagem. Nos diferentes ensaios de extração realizados e de quantificação de pigmentos fotossintéticos e proteína total, obtiveram-se rendimentos máximos de 1,95 ± 0,01 mgclorofila.gbiomassa fresca⁻¹, 0,83 ± 0,01 mgcarotenoides.gbiomassa fresca⁻¹ e 151 ± 9 mgproteína.gbiomassa fresca⁻¹ para clorofilas, carotenoides e proteína total, respetivamente. Verificou-se também a correlação entre os fatores abióticos e condições de cultivo com a produção de pigmentos fotossintéticos pela Ulva sp., que terá elevado impacto na sua qualidade final. Não foi possível validar a escala de cor utilizada como referência para o processamento da Ulva rigida na ALGAplus, devido a diferenças significativas (p

Dissertação de mestrado em Agricultura Biológica, apresentada à Escola Superior Agrária do Instituto Politécnico de Viana do Castelo O estágio académico enquadrado no Mestrado em Agricultura Biológica foi realizado num período de cerca de seis meses, na empresa Ingrediente Positivo. A Ingrediente Positivo é uma empresa de produção e comercialização de hortícolas em Modo de Produção Biológico (MPB), com um total de cerca de 5000 m2 de terreno de cultivo. Os principais objetivos desta componente foram a preparação da mestranda para o exercício da profissão de produtora de hortícolas em MPB, a identificação de desafios e oportunidades neste setor agrícola e a resolução de eventuais constrangimentos da empresa e/ou propostas de melhoria/ampliação de atividades desta. Durante o estágio foram acompanhadas todas as fases de produção – fertilização, rega, plantio/sementeira, prevenção e combate a pragas e doenças, colheita –, bem como pós-colheita e comercialização. Em termos de produção, os principais desafios foram o combate a pragas de lesmas e caracóis, de roedores e da doença míldio. São propostas consociações como medida preventiva de pragas e doenças. As principais dificuldades que a empresa enfrenta são a falta de mão-de-obra, a falta de formação e experiência das colaboradoras, a micro dimensão das produções e o aumento dos custos de produção. As principais oportunidades são o facto de a Agricultura Biológica (AB) ser um setor em crescimento, sendo objetivo da União Europeia aumentar 25%, até 2030, os terrenos de AB e Portugal também ter metas nesse sentido. Apesar das dificuldades, a empresa tem crescido e tem atualmente várias parcerias com várias empresas. Assim, pode dizer-se que vale a pena investir em Agricultura Biológica. Com este estágio, a mestranda adquiriu experiência e conhecimentos e cumpriu os objetivos delineados. The academic internship as part of the Master in Organic Agriculture was carried out over a period of about six months, at the company Ingrediente Positivo. Ingrediente Positivo is a company that produces and sells vegetables in Organic Production Mode (MPB), with a total of around 5000 m2 of cultivated land. The main objectives of this component were the preparation of the master's student for the exercise of the profession of vegetable producer in MPB, the identification of challenges and opportunities in this agricultural sector and the resolution of possible constraints of the company and/or proposals for improvement/expansion of activities of this. During the internship, all stages of production were monitored – fertilization, irrigation, planting/seeding, preventing and combating pests and diseases, harvesting – as well as post-harvesting and marketing. In terms of production, the main challenges were combating pests of slugs and snails, rodents and the downy mildew disease. Intercropping is proposed as a preventive measure against pests and diseases. The main difficulties that the company faces are the lack of manpower, the lack of training and experience of the collaborators, the micro dimension of the productions and the increase in production costs. The main opportunities are the fact that Organic Agriculture (AB) is a growing sector, and the European Union's objective is to increase it by 25%, by 2030, the lands of AB and Portugal also have goals in this sense. Despite the difficulties, the company has grown and currently has several partnerships with several companies. Thus, it can be said that it is worth investing in Organic Agriculture. With this internship, the master's student acquired experience and knowledge and fulfilled the outlined objectives.

Disserta??o de mestrado apresentada para obten??o do grau de Mestre em Atividades de Fitness, na Escola Superior de Desporto e Lazer do Instituto Polit?cnico de Viana do Castelo. Com a pandemia devido da COVID-19, em que foi obrigat?rio o isolamento social e confinamento de toda a popula??o, o presente estudo teve como objetivo investigar o impacto que o confinamento teve na qualidade de vida, na composi??o corporal e n?veis de sedentarismo nas crian?as dos 3 aos 12 anos segundo a perspetiva dos pais e professores. Para tal foi constru?do e aplicado um question?rio (em dois momentos) aos pais e encarregados de educa??o e realizaram-se ainda tr?s focus group a professores de nata??o e aos pais e encarregados de educa??o. Perante os resultados do question?rio ? poss?vel concluir que o confinamento teve impacto na qualidade de vida das crian?as, especialmente no que toca ? rotina, horas de sono e n?veis de atividade f?sica (AF), existindo diferen?as entre as faixas et?rias analisadas e para o fator ser filho ?nico. Contudo, e segundo a opini?o e perce??o dos pais e encarregados de educa??o, os n?veis de AF durante o confinamento aumentaram, dado este que vai no sentido contr?rio aos resultados obtidos atrav?s do question?rio. O facto de os pais passarem mais tempo com os filhos durante o confinamento e aquilo que ? a perce??o individual, pode estar a influenciar os resultados, visto que os dados quantitativos contrariam essa perce??o. A gest?o do dia-a-dia, o aumento do tempo despendido no ecr? e a diminui??o de horas de sono foram vetores que tamb?m sofreram um impacto significativo e que se agravaram do primeiro para o segundo confinamento. Os professores, evidenciaram a perce??o de mudan?as no comportamento das crian?as, aumento do n?vel de gordura corporal e menor concentra??o nas tarefas propostas, quando do regresso ?s atividades presenciais. Ficou comprovado tamb?m que a envolv?ncia familiar nas atividades das crian?as teve um papel decisivo para que os comportamentos mais sedent?rios diminu?ssem, assim como as caracter?sticas da habita??o, a exist?ncia de espa?o exterior para a realiza??o de atividades, influenciou positivamente a vida destas crian?as. Com esta investiga??o comprova-se que o confinamento influenciou de forma negativa os n?veis de AF e horas de sono, havendo um aumento do tempo despendido no ecr?. Conclui-se tamb?m que, a envolv?ncia familiar nas atividades com a componente motora, teve um papel decisivo para que os n?veis de sedentarismo baixassem. In long periods without school, children tend to adopt more sedentary behaviors. Knowing that we are in a pandemic situation due to COVID-19, in which social isolation and confinement of the entire population was mandatory, This study aimed to investigate the impact that confinement had on quality of life, body composition and levels of sedentary lifestyle in children aged 3 to 12 years, from the perspective of parents and teachers. To this end, a questionnaire was designed and applied (in two moments) to parents and swimming teachers along with three focus groups. Based on the results of the questionnaire, we can conclude that confinement had an impact on the quality of life of children, especially regarding to routine, hours of sleep and levels of physical activity (PA), with differences between the age groups analyzed and the fact of being only child. However, and according to the opinion of parents, PA levels during confinement increased, giving a different insight, as this goes against the results obtained through the questionnaire. The fact that parents spend more time with their children during confinement and what is the individual perception may be influencing the results, as the quantitative data contradict this perception. Day-to-day management, increased time spent on the screen and decreased sleep hours were vectors that also had a significant impact and that declined from the first to the second confinement. Teachers revealed a perception that children's behavior changed, with increased body fat level and less concentration on the proposed tasks, after returned to face-to-face activities. It was also proven that family involvement in children?s activities play a decisive role in reducing more sedentary behavior. Housing characteristics, the existence of outdoor space for carrying out activities, also positively influenced the lives of these children. This research demonstrates that confinement negatively influenced levels of PA and hours of sleep, with a consequent increase in time spent on screen. It was also concluded that the family involvement in activities with motor component of children played a decisive role in lowering the levels of sedentary lifestyle.

Immune-mediated endocrinopathies are among the most frequent immune-related adverse events (irAEs) with immune checkpoint inhibitors (ICIs) targeting programmed death-ligand 1 (PD-L1)/PD-1. However, the development of auto-immune diabetes is an uncommon event during PD(L)-1 blockade, either as monotherapy or in combination therapy. Here we report a case of a 75-year-old male with a mediastinal recurrence from a stage IA squamous cell carcinoma of the lung previously treated with stereotactic body radiotherapy (SBRT) who early developed a severe diabetic ketoacidosis (DKA) caused by new-onset auto-immune diabetes, with positive glutamic acid decarboxylase (GAD65) autoantibodies, during durvalumab consolidation therapy after concurrent chemoradiation. The patient had no personal or family history of diabetes or auto-immune diseases and was admitted after the second cycle of durvalumab to the intensive care unit (ICU) with severe DKA. During his hospitalization, insulin and fluid therapy were started and the patient had a favorable clinical course. Durvalumab treatment was interrupted and thyroiditis was verified during follow-up, without anti-thyroid antibodies, that progressed to subsequent hypothyroidism with need of thyroid hormone replacement therapy. This case highlights the rare irAE of autoimmune type 1 diabetes during anti-PD(L)-1 therapy, which can be life-threatening and requires adequate patient education and prompt medical treatment within a multidisciplinary team, including endocrinology and emergency medicine. Besides its low incidence, this case show how irAE must be taken in account about decision of ICI treatment, especially in curative setting, as they can be potentially fatal and impair overall survival. Furthermore, as reported in the present case, multiple endocrine irAEs can occur in the same patient either simultaneously or sequentially, suggesting that active surveillance is needed in those who develop endocrinopathies as a result of ICI treatment. Immune-mediated endocrinopathies are generally irreversible and cause life-long morbidity, which must be taken into consideration when deciding on further lines of treatment.

Aim: To evaluate the influence of YB-1 rs10493112 variant as a genetic marker for response to second-generation androgen receptor axis-target agents. Methods: A hospital-based cohort study of 78 patients with metastatic castration-resistant prostate cancer was conducted. Genotyping was performed by TaqMan®allelic discrimination technology. Main results: In abiraterone-treated and high-risk patients, YB-1 rs10493112 AA genotype carriers showed lower progression-free survival than C allele genotype patients (4 vs 17 months; p = 0.009). For carriers of AA genotype, multivariate Cox regression analysis revealed a fivefold increased risk of progression (p = 0.035). Conclusion: The study findings suggest that, for metastatic and castration-resistant prostate cancer patients, this polymorphism might be a putative marker for the clinical outcome.

Recent literature suggests that anthropogenic stressors can disrupt ecologically relevant behaviours in fish, such as the ability to escape from predators. Disruption of these behaviours at critical life history transitions, such as the transition from the pelagic environment to the juvenile/adult habitat, may have even greater repercussions. The literature suggests that an increase in temperature can affect fish escape response, as well as metabolism; however, few studies have focused on the acute sensitivity responses and the potential for acclimation through developmental plasticity. Here, we aimed at evaluating the acute and long-term effects of exposure to warming conditions on the escape response and routine metabolic rate (RMR) of early life stages of the white seabream, Diplodus sargus. Additionally, as food availability may modulate the response to warming, we further tested the effects of long-term exposure to high temperature and food shortage, as individual and interacting drivers, on escape response and RMR. Temperature treatments were adjusted to ambient temperature (19°C) and a high temperature (22°C). Feeding treatments were established as high ration and low ration (50% of high ration). Escape response and RMR were measured after the high temperature was reached (acute exposure) and after 4 weeks (prolonged exposure). Acute warming had a significant effect on escape response and generated an upward trend in RMR. In the long term, however, there seems to be an acclimation of the escape response and RMR. Food shortage, interacting with high temperature, led to an increase in latency response and a significant reduction in RMR. The current study provides relevant experimental data on fishes’ behavioural and physiological responses to the combined effects of multiple stressors. This knowledge can be incorporated in recruitment models, thereby contributing to fine-tuning of models required for fisheries management and species conservation.

Relatório de Estágio do Mestrado Integrado em Ciências Farmacêuticas apresentado à Faculdade de Farmácia A prevalência de micotoxinas no meio ambiente está associada à potencial contaminação dos alimentos, principalmente no que respeita aos cereais, o que resulta num inevitável aumento da exposição humana. O arroz, sendo o segundo cereal mais consumido no mundo, constitui uma importante fonte de contaminação por micotoxinas, podendo sercontaminado por várias micotoxinas simultaneamente.Devido à elevada frequência de alertas de contaminação por micotoxinas notificadas pelo portal Rapid Alert System for Food and Feed (RASFF) da Comissão Europeia, e à publicação de estudos que reportam níveis de micotoxinas acima dos limites regulamentares estabelecidos, este trabalho pretendeu fazer uma compilação de alguns desses estudos erever as principais técnicas utilizadas na deteção e quantificação destes compostos em arroz. Para além disso foi realizada uma revisão dos efeitos tóxicos associados à contaminação por micotoxinas, bem como das técnicas utilizadas atualmente na tentativa de mitigar essa contaminação.Apesar de um grande número de países já ter uma legislação definida com limites máximos definidos, existe ainda um longo caminho a percorrer nesta área, tendo sido este tema considerado uma prioridade pela Autoridade Europeia para a Segurança Alimentar (European Food Safety Authority, EFSA), sobretudo devido à crescente preocupação com asalterações climáticas, que favorecem o desenvolvimento de fungos em alimentos e potenciam a contaminação desses mesmos alimentos por micotoxinas. É necessário um maior número de estudos para poder definir a real exposição da população, bem como as consequências e potenciais sinergismos relacionados com a co-ocorrência de micotoxinas.Este trabalho inclui ainda dois relatórios de estágio, sob a forma de uma análise SWOT, com base nos Estágios Curriculares que realizei em Farmácia Comunitária, na Farmácia Central, e em Indústria Farmacêutica, nos Laboratórios Basi. The prevalence of mycotoxins in the environment is associated with potential crops contamination, mostly cereals, which results in an unavoidable increase of human exposure. Rice, being the second most consumed cereal worldwide, constitutes an important source of contamination by mycotoxins, since it is usually contaminated with several mycotoxins simultaneously.Due to the increasing number of notifications reported by the Rapid Alert System for Food and Feed (RASFF) portal of the European Commission, and the publication of studies that reported the occurrence of mycotoxins in levels above the legislated limits, this work intends to compile some of those studies and review the main methods used on the detection and quantification of these compounds in rice. Moreover, the toxic effects associated with mycotoxins contamination were also reviewed, along with the techniques used in order to attempt to minimize that contamination.Although a great number of countries already have defined legislation with specified maximum levels, much more needs to be done, since this thematic was considered by the European Food Safety Authority (EFSA) as a priority, mostly due to the growing concern with climatic changes. Climatic changes are proven to be associated with fungal growth in foodstuffs, leading to mycotoxins’ contamination. Further investigation is still required to establish the real exposition to these contaminants, as well as the consequences and possible synergistic effects due to the co-occurrence of mycotoxins.This work also includes two internship reports, in the form of a SWOT analysis, based on the Curricular Internships that I performed in Community Pharmacy, at Farmácia Central, and in Pharmaceutical Industry, at Laboratórios Basi.

Nitrate contamination of groundwater is an important public health issue worldwide. For environmental and public health reasons, water should not contain more than 50 mg/L NO3. An aquifer for which this limit is exceeded can be designated as a nitrate vulnerable zone (NVZ) and subject to action programs to minimize the NO3 input. The study aims to assess future trends of groundwater quality and to predict the time required for groundwater to achieve the environmental goals in the Esposende–Vila do Conde NVZ (Portugal). Flow and transient nitrate transport modelling were performed using the FEFLOW software. The numerical model represents the saturated zone of phreatic aquifers, designed in a three-dimensional three-layer model. The calibration process was completed through the tool FEPEST. Sensitivity analysis was performed to investigate the model response to changes in hydraulic parameters and aquifer recharge. Two major simulations of mass transport were performed considering different options on nitrogen loads: (1) agricultural nitrogenous loads of diffuse origin; (2) nitrogen loads from agricultural and livestock sectors together. The results show that the minimization measures imposed in the NVZ are effective, shown by the groundwater nitrate concentration decreasing over time; however, concentrations above 50 mg/L will persist for the next two decades in both simulated scenarios. Combining the conceptual hydrogeological model, geovisualization techniques, and numerical flow and mass transport modelling has been shown as a comprehensive approach to understanding the measures needed for sustainable water resources management and particularly to predicting hydraulic heads and NO3 dispersion in aquifers.

Nitrogen is necessary for the synthesis of a large number of compounds, such as amino acids, which are very important for several yeast dynamics, and for industrial purposes. This project aims at the identification and characterization of new transporters involved in the excretion of amino acids through the study of a specific gene of the MFS superfamily of transporters in eukaryotes, the DTR1 gene. In the MDR-MFS (MultiDrug Resistance-Major Facilitator Superfamily) family of proteins, up to 100 are unknown, 24 proteins are Multidrug Resistance, and may be involved in the excretion of amino acids. DTR1 is a multi-drug resistance protein with a physiological role assigned to the yeast cell where the layers of chitosan and dityrosine on the external spore wall provide greater resistance to environmental stresses. In the present study, techniques such as E. coli and S. cerevisiae transformation, PCR, RT-PCR, qPCR analysis were applied. The characterization of the DTR1 gene was carried out through its location and expression using various tools, such as the Green Fluorescent Protein by observing when it is expressed in the control of its own promoter, and a fusion with the GAL1 promoter, verifying overexpression of the protein or whether the expression is normally done. The construction of the PGAL1-DTR1-GFP cassette did not occur as expected, therefore was not possible to observe the overexpression of the DTR1 gene and its location. As for characterization on a plasmidic level, plasmids containing the GAP1 promoter were used and the promoter induced, this was possible to observe the expression analysis of the GAP1-DTR1 construction with a change of medium. Additionally, a study of the influence of glycerol and temperature, on S. cerevisiae culture was carried out where it was observed that high temperature and presence of glycerol might be stress conditions enough to DTR1 to be expressed. O azoto é um nutriente mineral crítico em todos os organismos vivos, pois é necessário para a síntese de um grande número de compostos, incluindo hormonas, nucleotídeos e aminoácidos. Os aminoácidos são muito importantes para várias dinâmicas da levedura, como síntese de proteínas, metabolismo de hormonas, transmissão nervosa, crescimento celular, geração de energia, metabolismo do azoto e síntese de bases azotadas. Os aminoácidos também são importantes para fins industriais, como aplicações em alimentos como o aminoácidos glutamato (intensificador de sabor) ou aspartato, fenilalanina (adoçantes); para alimentação, tais como os aminoácidos lisina, metionina, treonina; e para aplicações farmacêuticas, como soluções de infusão e blocos de construção, triptofano (indutor do sono) e fenilalanina (antidepressivo). Este projeto visa a identificação e caracterização de novos transportadores envolvidos na excreção de aminoácidos por meio do estudo de um gene específico da superfamília MFS (Major Facilitator Superfamily) de compostos azotados em eucariontes, o gene DTR1. De acordo com, Sá-Correia et al., (2009) vários transportadores MDR foram identificados e estudados em diferentes organismos, particularmente aquele pertencentes à superfamília ATP-binding cassette (ABC). Um grupo de transportadores envolvidos na resistência a multidrogas menos caracterizado pertence à família MFS-MDR. Proteínas deste grupo têm vindo a receber mais atenção, maioritariamente em bactéria. Em Saccharomyces cerevisiae, a maioria dos membros desta família foi apenas descobertos aquando revelada a sequencia genómica desta levedura e caracterizados em alguns aspetos nos últimos 12 anos. A família MDR-MFS (Multidrug Resistance-Major Facilitator Superfamily) de proteínas tem aproximadamente 300 proteínas transportadoras de membrana, com até 100 sendo desconhecidas. 24 proteínas são MDR da superfamília MFS, algumas delas podem estar envolvidas na excreção de aminoácidos, como DTR1. Dentro da DHA1 (Drug: H+ antiporter family 1) que é composta por 46 proteínas, sete genes são individualmente necessários para conferir resistência à quinidina, um fármaco antiarrítmico e anti malária, entre esses genes encontram-se os genes AQR1, TPO1 e DTR1. Estes sete transportadores podem, também, proteger a célula contra outros compostos que estão normalmente ausentes no ambiente natural de células de levedura e poderão ter substratos fisiológicos específicos, onde fármacos são transportados fortuitamente ou oportunisticamente. Ainda na família DHA1, 9 proteínas mostraram ser bombas de multirresistência, 15 são provavelmente bombas de efluxo específicas para drogas e 22 são proteínas hipotéticas ou não caracterizadas. Os genes de resistência a múltiplas drogas, isto é, a aquisição simultânea de resistência a uma variedade de químicos citotóxicos, é encontrada numa grande variedade de organismos, desde bactérias a mamíferos, e isto poderá causa um severo problema clínico, principalmente no tratamento de cancros humanos e infeções de origem bacteriana e fúngica, tendo atingido proporções alarmantes nos últimos anos. DTR1 é a primeira proteína de resistência a múltiplas drogas da superfamília de facilitadores principais com um papel fisiológico atribuído na célula de levedura, mas o transporte por DTR1p pode não ser restrito ao seu substrato natural, bisformil ditirosina. As camadas de quitosana e ditirosina da parede externa dos esporos conferem maior resistência a estresses ambientais no esporo, incluindo a capacidade de passar pelo trato digestivo dos insetos, permitindo a dispersão para o meio ambiente. No presente estudo é realizada a caraterização do gene DTR1 através da sua localização e expressão com recurso a variadas ferramentas, tais como a proteína Green Fluorescent Protein, produzida pelo cnidário Aequorea victoria que emite fluorescência na zona verde do espectro visível, observando quando esta está a ser expressa no controle de seu próprio promoter. Uma fusão com o promotor Gal1, um operão procariótico que codifica as enzimas necessárias para o metabolismo da galactose, para verificar a sobrexpressão da proteína ou se a expressão é feita normalmente como no seu estado natural. Com essas fusões podemos analisar se a proteína está bem expressa, superexpressa e onde ela ocorre na célula e, usando plasmídeos identificados como GAP1 e UGA4 ambos contendo o promotor GAP1, ativado pela falta de azoto, para observação do comportamento do gene em estudo. Essas ferramentas contribuem para a caracterização a nível genómico e plasmídico da proteína de interesse, DTR1, para compreender sua função e localização no genoma da levedura. O objetivo principal deste trabalho foi construir uma cassete combinando primers para o plasmídeo, primeiro e para localização do DTR1 foi utilizado o plasmídeo PKT140, que contém a proteína GFP e resistência à canamicina. Os primers construídos são homólogos à proteína GFP, no caso do F5, e à resistência à canamicina, no caso do primer R3. A estratégia adotada foi a utilização de um fragmento do plasmídeo contendo os primers, GFP e a resistência à canamicina, que foi amplificado com um tamanho esperado de 2515 pares de bases e transformado em S. cerevisiae. A tag GFP foi aplicada a jusante do gene de interesse, DTR1. A canamicina foi usada como marca de seleção no momento da transformação e a integração no genoma da levedura foi concluída por recombinação homóloga. Para compreender se a proteína de interesse, DTR1, está a ser superexpressa, um fragmento do plasmídeo PGAL1 foi usado, novamente os primers foram construídos contendo parte de DTR1 e o promoter Gal1 no caso do iniciador R2 e parte de DTR1 e resistência à nourseotricina em o caso do primer F4. Para análise deste estudo recorreu-se a técnicas como transformação em E. coli e em S. cerevisiae, digestão com recurso a enzimas de restrição, PCR, RT-PCR (Reverse Transcriptase PCR), análise de expressão por qPCR (Quantitative Real Time PCR), Cross-feeding e ainda Western Blotting. O fragmento do plasmídeo mais primers foi amplificado por PCR com 1850 pares de bases e a integração no genoma da levedura é feita por recombinação homóloga. A nível funcional concluímos que não foi possível a construção da cassete PGAL1-DTR1-GFP tendo sido efetuadas várias tentativas da mesma não tendo sido possível a observação da sobrexpressão do gene DTR1 e a sua localização. A nível plasmídico foi possível uma observação de analise de expressão de diferentes estudos de comportamento da construção GAP1-DTR1 com alteração de meio. Foi ainda feito um estudo de influência do glicerol e temperatura em cultura de S. cerevisiae em meio mínimo. Tendo em conta que o gene DTR1 faz parte da reprodução de S. cerevisiae através esporulação em condições de stress, verificou-se que uma temperatura de 30ºC e glicerol 10% poderão representar condições de stress suficientes para que este gene seja expresso.

Tetracosapolyenoic fatty acids (TPA), namely tetracosapentaenoic (24:5n-6) and tetracosahexaenoic (24:6n-3) acids, are chemotaxonomic biomarkers of octocorals (Cnidaria, Octocorallia) in the marine environment. The present study confirms the occurrence of TPA on a marine non-cnidarian taxon, the nudibranch mollusc Armina maculata. This discovery is explained by the specialized feeding regime (stenophagy) of this sea slug that preys upon the octocoral Veretillum cynomorium. The prevalence of 24:5n-6 and 24:6n-3 in the body of A. maculata was demonstrated through the analysis of specimens starved for 30 days in captivity. Since the time frame is superior to the residence time of ingested octocorals, the present findings provide empirical evidence that the reported TPA are allocated from nudibranch tissues. Here we found support for previous claims that the presence of TPA in the marine tree of life is not restricted to its lower branches, as stenophagous trophic interactions may allow its transfer to or posterior biosynthesis in more evolved taxa.

Parabens are widely used as antimicrobial and preservative in pharmaceutical and personal products. Their presence has been detected in rivers and wastewater treatment plants. Photocatalytic ozonation process using a low amount of 0.1 wt% Ag-TiO2 proved to be efficient on the degradation of a mixture of five parabens using a low transferred ozone dose (TOD). The pH effect was analyzed under acidic and neutral conditions. Also, the effect of hydroxyl radical scavenger on parabens degradation and on by-products formation was discussed. Hydroxyl radical present a significant role over parabens degradation and also on by-products formation. The reaction mechanism was analyzed using municipal wastewater as a matrix to infer about the behavior of the process at actual conditions. Municipal wastewater as a matrix clearly enhanced the parabens degradation when compared with the case where ultrapure water was used. In fact, the TOD required for total parabens degradation is lowered 10–20 mg/L of TOD. Therefore, to understand the main responsible species for this improvement, the effects of several ions naturally present in wastewater (HCO3−, Cl− and SO42−) were tested. According to the results it seems that sulfate radical improves the process, while chloride and bicarbonate radicals decrease the process efficiency. In terms of toxicity the luminescence inhibition for Vibrio fischeri was analyzed. The inhibition significantly decreased for treated spiked municipal wastewater.

Sharks have been facing unprecedented pressure over the last decades, and ocean acidification may represent an additional threat, particularly during their most susceptible life stages. Hence, the ...

Wildfires are a recurrent and increasing threat in Mainland Portugal, where over 4,500 thousand hectares of forests and shrublands have burned in the last 38 years. Landscapes affected by those wildfires have suffered an increase of soil erosion processes, which can negatively affect soil carbon storage, reduce fertility, forest productivity, and become a source of pollutants. Taking these in mind, the main objective of this study is to offer a ground base of post-fire soil erosion risk determination for Mainland Portugal, which will provide a set of tools to help forest managers in the post-fire decision-making, and therefore adequately implement mitigation measures to prevent such impacts.Post-fire soil erosion was assessed by the applying the semi-empirical soil erosion model Revised Morgan–Morgan–Finney(Morgan, 2001), to the entire Portuguese forest and shrubland areas according to distinct scenarios (burn severity, climate). This study benefits from the use of several reliable official datasets of soil characteristics, as also from several model calibrations and validation with field data collected in the last 10 years for the 1st and 2nd post-fire years. The obtained soil erosion map identifies areas with higher post-fire erosion risk in the past and for future climate extremes. Findings of this study will be a valuable tool for forest managers to minimize the economic and environmental losses of vegetation fires in Portugal.AcknowledgementsThis work was supported and conducted in the framework of the FEMME project (PCIF/MPG/0019/2017) funded by FCT - Portuguese Foundation for Science and Technology. The study was also supported by: i) National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020; and, ii) National Funds by FCT - Portuguese Foundation for Science and Technology, under the project UID/AMB/50017/2019. Data were provided by the European Forest Fire Information System – EFFIS (http://effis.jrc.ec.europa.eu) of the European Commission Joint Research Centre.

As in any other Mediterranean region, wildfires highly affect Portuguese forests leading to substantial economic and ecological losses. Wildfires are also an important cause for the degradation of forest ecosystem services, which are responsible for the maintenance of water quality, flooding and soil erosion control. Considering that wildfire frequency is expected to increase in the future due to changes in climate and socio-economic drivers, future land degradation cause by wildfires is considered with great concern.To tackle the impacts of wildfires on the affected ecosystems such as soil erosion is highly recommendable the use of adequate post-fire management practices for its mitigation. However, the dimension of the area affected by the 2017 wildfires in Portugal (500 thousand ha) showed the impossibility to effectively treat a nation-wide burned area for erosion control. Given this context, hydrological modelling arises as a key–tool for post-fire land management decision making, by identifying potential on-and-off-site post-fire impacts, and by allowing the selection of target areas with a higher soil erosion risk for the implementation of mitigation treatments.To address this problem, the ESP team - under the FEMME project - defined the strategy of using soil erosion models at hillslope scale to address on-site impacts at the national level and catchment scale models to address off-site impacts. A national soil erosion risk map in case of a wildfire will help land managers to choose the priority areas for the implementation of emergency stabilization measures. While continuous and event-based hydrological models, will allow assessing the risks of water quality degradation and the occurrence of extreme hydrological events, which can impact downstream values-at-risk.To understand if the chosen model approach is adequate to the problem in hand since it resulted in outputs with distinct spatial and temporal scales, we have decided to perform an evaluation focused on scales and model adaptations to burned areas. We were able to conclude that simple empirical models such as the Morgan-Morgan-Finney [1], which can provide predictions at hillslope and seasonal-to-annual scale, are well adapted to post-fire conditions and are useful to identify high risk areas for the implementation of mitigation treatments. On the other side, their simplicity does not allow to determine the risk of flooding or water bodies contamination, outside the burned area, and under specific rainfall events which implies a daily or sub-daily time-steps. For that purpose, spatially-explicit process-based such as SWAT [2] or LISEM [3] can provide a more detailed feedback, although there have been few model adaptations to burned conditions at this scale, especially considering the implementation of post-fire mitigation measures. [1] Morgan, R., 2001. A simple approach to soil loss prediction: a revised Morgan–Morgan–Finney model. Catena 44 (4): 305–322. https://doi.org/10.1016/S0341-8162(00)00171-5[2] Arnold, J.G., Fohrer, N., 2005. SWAT2000: Current capabilities and research opportunities in applied watershed modelling. Hydrol. Process. 19, 563–572. https://doi.org/10.1002/hyp.5611[3] De Roo APJ, Wesseling CG, Ritsema CJ (1996) LISEM: a single‐event physically based hydrological and soil erosion model for drainage basins. I: theory, input and output. Hydrological processes 10, 1107–1117.

Introdução: O surgimento da dentição decídua na cavidade oral é uma característica comum do desenvolvimento da criança. A erupção precoce dos dentes no recém-nascido pode dar origem a diversas complicações na cavidade oral como é o caso da doença de Riga-Fede, que representam um motivo de preocupação para os pais e um desafio para os profissionais de saúde. Objetivo: Realizar uma revisão sistemática integrativa sobre os dentes natais e neonatais e a doença de Riga-Fede. Conhecer os mecanismos pelos quais acontecem, desde a sua etiologia até aos planos de tratamento a considerar, após a execução de um diagnóstico adequado. Materiais e Métodos: Com recurso às bases de dados PubMed e SciElo, foi realizada uma pesquisa bibliográfica utilizando as seguintes palavras-chave: “Riga-Fede”; “Natal and Neonatal teeth and Riga-fede”; “Natal and neonatal theeth”; “Riga-fede desease causes” and “Riga-Fede Diagnosis” entre 2011 e 2021. Foram encontrados 375 artigos dos quais foram considerados 21 artigos. Discussão: Os dentes natais e neonatais podem originar diversas complicações na cavidade oral do recém-nascido, mais comumente a doença de Riga-Fede. Após a sua deteção clínica é crucial intervir, de forma a prevenir e/ou tratar patologias. Existem preferencialmente dois tipos de tratamento eleitos, o tratamento conservador e a exodontia. Conclusão: A ocorrência dos dentes natais e neonatais é uma condição rara e de etiologia ainda pouco conhecida. Segundo a literatura, a doença de Riga-Fede é caracterizada como a complicação mais comum associada à presença destes dentes.

Relatório de Estágio do Mestrado Integrado em Ciências Farmacêuticas apresentado à Faculdade de Farmácia The discovery of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), an adaptive defence system for bacteria against viral infections, allowed the creation of a technology called CRISPR/Cas9 system that came to revolutionize genome editing technologies. This system has stood out due to its versatility, simplicity and efficiency in genetic manipulation. Research carried out over the past few years has proven the usefulness and potential of this technology for therapeutic applications, particularly in gene therapy. The potential of the CRISPR/Cas9 system has been progressing thanks to the increasingly detailed knowledge of the mechanisms of this system and the genome editing strategies used. However, there are still many challenges that have to be overcome in order for this technology to be considered safe and effective in the treatment of many pathologies. The discovery of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), an adaptive defence system for bacteria against viral infections, allowed the creation of a technology called CRISPR/Cas9 system that came to revolutionize genome editing technologies. This system has stood out due to its versatility, simplicity and efficiency in genetic manipulation. Research carried out over the past few years has proven the usefulness and potential of this technology for therapeutic applications, particularly in gene therapy. The potential of the CRISPR/Cas9 system has been progressing thanks to the increasingly detailed knowledge of the mechanisms of this system and the genome editing strategies used. However, there are still many challenges that have to be overcome in order for this technology to be considered safe and effective in the treatment of many pathologies. A descoberta de Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), um sistema de defesa adaptativo de bactérias contra infeções virais, permitiu a criação de uma tecnologia denominada sistema CRISPR/Cas9 que veio revolucionar as tecnologias de edição do genoma. Este sistema tem-se destacado devido à sua versatilidade, simplicidade e eficiência na manipulação genética. As investigações realizadas ao longo dos últimos anos têm vindo a comprovar a utilidade e o potencial desta tecnologia para aplicações terapêuticas, particularmente na terapia génica. O potencial do sistema CRISPR/Cas9 tem vindo a progredir graças ao conhecimento cada vez mais pormenorizado dos mecanismos deste sistema e das estratégias de edição do genoma utilizadas. Existem, no entanto, ainda muitos desafios que têm de ser superados para que esta tecnologia possa ser considerada segura e eficaz no tratamento de muitas patologias. A descoberta de Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), um sistema de defesa adaptativo de bactérias contra infeções virais, permitiu a criação de uma tecnologia denominada sistema CRISPR/Cas9 que veio revolucionar as tecnologias de edição do genoma. Este sistema tem-se destacado devido à sua versatilidade, simplicidade e eficiência na manipulação genética. As investigações realizadas ao longo dos últimos anos têm vindo a comprovar a utilidade e o potencial desta tecnologia para aplicações terapêuticas, particularmente na terapia génica. O potencial do sistema CRISPR/Cas9 tem vindo a progredir graças ao conhecimento cada vez mais pormenorizado dos mecanismos deste sistema e das estratégias de edição do genoma utilizadas. Existem, no entanto, ainda muitos desafios que têm de ser superados para que esta tecnologia possa ser considerada segura e eficaz no tratamento de muitas patologias.

O Estágio Profissional assume-se como uma etapa fundamental e crucial no culminar da formação inicial. O Estudante-Estagiário vivencia, na sua plenitude, as exigências inerentes ao exercício da profissão docente através, não só das funções que desempenha, como também das experiências de partilha, de reflexão constante, de obstáculos e desafios que emergem da prática que, acima de tudo, se demonstram fulcrais para a construção da identidade profissional e desenvolvimento na profissão docente. Mais ainda, importa referir que a dimensão reflexiva atingiu o auge no Estágio Profissional, no qual a autora foi "chamada" a refletir desde o início, até ao final do ano letivo, tendo contribuído, também, para o desenvolvimento da autonomia profissional da mesma. O presente documento visa transparecer a descoberta do mundo da profissão docente e o caminho traçado pela transparência de uma estudante-estagiária que viveu e sentiu o ano de Estágio Profissional e, consequente concretização de um sonho, com as emoções e sentimentos constantemente à flor da pele, tal como a mesma vive e sente o mundo que a rodeia. O percurso trilhado nesta descoberta, envolveu a passagem por diferentes etapas, através das quais foi possível experienciar momentos distintos, fortemente marcados por altos e baixos, derrotas e vitórias, lágrimas e sorrisos, que permitiram retirar ilações importantes para o processo de ensino-aprendizagem, especificamente ao nível da sua conceção, planeamento, realização e avaliação, pelo que os alunos caminharam, lado a lado, de mãos dadas com a autora. Não menos importante, o ano letivo 2019/2020 ficou fortemente marcado pelo aparecimento, imprevisível e inesperado da Covid-19, evoluindo para uma pandemia mundial que exigiu o encerramento das escolas em Portugal. Em tempos de incerteza, insegurança e inquietação, a autora partilha os ajustes e (re)adaptações no processo de ensino-aprendizagem com o objetivo de garantir e assegurar a continuidade do ano letivo através do Ensino a Distância. The Practicum Training is assumed as a fundamental and crucial stage in the culmination of the initial training. The pre-service teacher experiences, in its fullness, the requirements inherent to the exercise of the teaching profession, through not only the functions he performs, but also throughout sharing, constant reflection, obstacles and challenges that emerge from the practice that, above of all, are essential for the construction of professional identity and development in the teaching profession. Furthermore, it should be noted that the reflective dimension reached its peak in the Practicum Training, in which the author was "called" to reflect from the beginning, until the end of the school year, having also contributed to the development of its professional autonomy. This document aims to show the discovery of the world of the teaching profession and the path traced by the transparency of a pre-service teacher who lived and felt, the year of Practicum Training and, consequently, the fulfillment of a dream, with emotions and feelings constantly rushing, as it lives and feels the world around her. The path followed in this discovery involved the passage through different stages, with which was possible to experience different moments, strongly marked by ups and downs, defeats and victories, tears and smiles, which allowed to draw important lessons for the teaching-learning process, specifically in terms of its conception, planning, realization and assessment, so the students walked side by side with the author. Last but not least, the academic year of 2019/2020 was strongly marked by the unpredictable and unexpected outbreak of Covid-19, which led to a worldwide pandemic that demanded the closure of schools in Portugal. Living times of uncertainty, insecurity and restlessness, the author shares the adjustments and (re)adaptations in the teaching-learning process, in order to guarantee and ensure the continuity of the school year through Distance Learning.

Biliary tract obstruction in cancer patients is usually associated with a poor prognosis. The obstruction may cause distressing symptoms, such as pruritus. As this situation occurs mostly in advanced cancer, the primary objective of the treatment is in many cases symptom control and not prolonging life. However, some patients can be candidates for chemotherapy. To see the outcomes of stenting insertion in patients of our oncology center. A retrospective study of patients who have undergone this procedure between 1 October 2011 and 31 December 2018 was carried out. Insertion of a biliary stent was performed in 171 patients. The most common diagnoses were gastric and colorectal cancers, each with 42 (24%), followed by pancreatic (34 (20%)) and biliary tract cancer (25 (14%)). Most stents (155 (91%)) were placed percutaneously. Complications were seen in 91 (53%) patients and the most common was cholangitis in 48 (53%) patients, and the median survival was 75.5 days (3–1246). A total of 168 (98%) patients were referred to palliative care. In a multivariable analysis, the ECOG performance status was associated with survival, with the ECOG 0, 1, and 2 associated with better survival and peritoneal metastases associated with lower survival. For many patients with advanced cancers, it may not be clear if the benefits of palliative biliary stents outweigh the risks. Therefore, the problem should be discussed with the patients and their families, making clear the goals of care and the potential benefits and risks that can be expected.

Submitted by azevedo@ufp.pt (azevedo@ufp.pt) on 2021-02-01T16:23:02Z No. of bitstreams: 1 PPG_33864.pdf: 1034952 bytes, checksum: 3e6981cd5585a0143733921f05e0a6a6 (MD5) Approved for entry into archive by azevedo@ufp.pt (azevedo@ufp.pt) on 2021-02-01T16:23:40Z (GMT) No. of bitstreams: 1 PPG_33864.pdf: 1034952 bytes, checksum: 3e6981cd5585a0143733921f05e0a6a6 (MD5) Made available in DSpace on 2021-02-01T16:23:40Z (GMT). No. of bitstreams: 1 PPG_33864.pdf: 1034952 bytes, checksum: 3e6981cd5585a0143733921f05e0a6a6 (MD5) Previous issue date: 2020-09-11

Schizophrenia remains a poorly understood disease, hence the interest in assessing and indirectly characterizing brain activity and connectivity. This paper aims to search for potential biomarkers in schizophrenia with functional magnetic resonance data, between subjects in the resting state. Firstly, we used fMRI from an open database, SchizConnect, of 48 subjects, in which 27 were control subjects, with no apparent disease and the others 21 were patients with schizophrenia. With the SPM tool, we proceeded to manually pre-process the images obtained, at the risk of having influenced the final results. Then, with the AAL atlas as a reference, we divided the brain into 116 areas. Then, brain activity in these areas were analysed, using the LEiDA method, which aims to characterize brain activity at each time point t by phase locking patterns of the BOLD signal. After the application of LEiDA, brain activity was evaluated based on trajectories and bar graphs of functional connectivity states in which the probability of occurrence and their dwell time were calculated for each state. It was also found that the visual cortex was the subsystem that showed significantly more probability of occurrence in schizophrenia patients to be assessed, and may correspond to symptoms of hallucinations by the patients with schizophrenia.

Recent metagenomics studies have revealed several arthropod species to be major reservoirs for RNA viruses. One of these reservoirs is Ixodes ricinus, the most prevalent tick species in Europe, which is known to be a vector for many viral and bacterial pathogens. For this study, we decided to investigate the virosphere of Belgian Ixodes ricinus ticks. High-throughput sequencing of tick pools collected from six different sampling sites revealed the presence of viruses belonging to many different viral orders and families, including Mononegavirales, Bunyavirales, Partitiviridae, and Reoviridae. Of particular interest was the detection of several putative human pathogens, including members of the families Nairoviridae and Phenuiviridae as well as three new reoviruses, two of which cluster together with members of the genus Coltivirus. One of these two viruses represents a new strain of Eyach virus, a known causative agent of tick-borne encephalitis. All genome segments of this new strain are highly similar to those of previously published Eyach virus genomes, except for the fourth segment, encoding VP4, which is markedly more dissimilar, potentially indicating the occurrence of an antigenic shift. Further PCR-based screening of over 200 tick pools for 11 selected viruses showed that most viruses could be found in all six sampling sites, indicating the wide spread of these viruses throughout the Belgian tick population. Taken together, these results illustrate the role of ticks as important virus reservoirs, highlighting the need for adequate tick control measures.

Over the last two decades, the identification of targetable oncogene drivers has revolutionized the therapeutic landscape of non-small cell lung cancer (NSCLC). The extraordinary progresses made in molecular biology prompted the identification of several rare molecularly defined subgroups. In this review, we will focus on the novel and emerging actionable oncogenic drivers in NSCLC. Recently, novel oncogene drivers emerged as promising therapeutic targets besides the well-established EGFR mutations, and ALK/ROS1 rearrangements, considerably expanding the list of potential exploitable genetic aberrations. However, the therapeutic algorithm in these patients is far less defined. The identification of uncommon oncogene drivers is reshaping the diagnostic and therapeutic approach to NSCLC. The introduction of novel highly selective inhibitors is expanding the use of targeted therapies to rare and ultra-rare subsets of patients, further increasing the therapeutic armamentarium of advanced NSCLC.

Tese de mestrado, Física (Astrofísica e Cosmologia), Universidade de Lisboa, Faculdade de Ciências, 2021 Submitted by Teresa Boa (tdboa@fc.ul.pt) on 2021-05-06T17:02:44Z No. of bitstreams: 1 ulfc126227_tm_Ana_Rita_Ribeiro.pdf: 1174951 bytes, checksum: 8ba8fd8570e507c89c33f21900869229 (MD5) Made available in DSpace on 2021-05-06T17:02:53Z (GMT). No. of bitstreams: 1 ulfc126227_tm_Ana_Rita_Ribeiro.pdf: 1174951 bytes, checksum: 8ba8fd8570e507c89c33f21900869229 (MD5) Previous issue date: 2021

Corbicula fluminea, an Asian clam, is one of the worst invasive species in Europe that can survive in very adverse environmental conditions. Despite its negative impacts, the species also has the capacity to bioaccumulate heavy metals, contaminants and can be exploited for wastewater treatment purposes. The capacity of the Asian clam to remove Escherichia coli, used as fecal contamination indicator, was analyzed. Conventional wastewater treatment plants are not suitable to remove bacteria, thus resulting in treated municipal wastewater with high bacterial loads. E. coli clearance rate was analyzed as function of the number of clams. The bivalves can remove bacteria until concentrations below the detection limit in about 6 h. The adsorption on the clam shells’ and bioaccumulation on the soft tissues were also analyzed. The depuration of clams along 48 h were analyzed revealing that no bacteria was detected in the water. Thus, these results suggest that Asian clam can bioprocess E. coli. On the other hand, results obtained by this methodology were compared with ozonation and photocatalytic oxidation using TiO2, Ag, Au, Pd-TiO2. In all treatments it was possible to achieve concentrations of E. coli below the detection limit. However, photocatalytic oxidation demands about 4700 folds more energy than ozonation, besides the costs associated with catalysts. Comparing complexity of ozonation with biofiltration, this study suggests that application of biofiltration using C. fluminea can be a suitable solution to minimize the presence of bacteria in wastewater, reducing environmental and economic impacts.