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In the present study, we suggest that the Myrciaria floribunda (West ex Willd.) O. Berg fruit (cambuí) is a natural source of potential molecules against COVID-19. Extracts from orange and purple cambuí were obtained by using pressurized hot water extraction (PHWE). Extraction overall yield was 39.66 ± 1.62% and 33.00 ± 8.44% for the extracts of purple and orange cambuí, respectively. All analyzed extracts showed high total phenolic contents, especially those of purple fruits (3870.37 ± 353.09 mg GAE/100g). The total flavonoid content was 693.16 ± 180.09 and 770.08 ± 103.62 mg RE/100g for purple and orange fruit extracts, respectively. The secondary metabolite profile exhibited ellagic acid, ellagitannins, and derivatives of caffeic acid and ellagic acid. PHWE showed a good alternative for molecules separation from cambuí with promising potential for further in vitro and in vivo evaluation against Covid-19 infections.

This study aimed to evaluate the Mangaba Genebank of Embrapa Tabuleiros Costeiros, Brazil, since its implementation in 2006 and characterize the accessions in the reproductive stage. We used 21 descriptors (botanical, morphological, growth, biometric, and physicochemical) to assess mangaba germplasm, as well as the data of enrichment and survival since the genebank implementation. There was a success in the enrichment, maintenance, and diversity of the germplasm evaluated. The results showed a significant difference between the accessions for most descriptors. The fruits presented good quality characteristics both for fresh consumption and processing. Moreover, these results supported the publication of the minimum descriptors for this species by Bioversity International and Embrapa. The knowledge of mangaba variability will subsidize conservation strategies and should encourage future researches on breeding.

The cambui tree (Myrciaria floribunda (West ex Willd) O. Berg), is a native fruit tree from Brazil, whose fruit present potential in natura and industrial consumption. Our objective was to evaluate the genetic diversity between individuals differentiated by fruit color - orange and purple, by using ISSR molecular markers. The plant material belongs to the Caju Private Natural Heritage Reserve, experimental field of Embrapa Coastal Tablelands, in the municipality of Itaporanga d’Ajuda, SE. Young leaves from 25 individuals (15 with orange color fruit and 10 with purple color fruits) were collected for DNA extraction and PCR-ISSR analysis with 15 primers, which produced a total of 93 bands, being 95.5% of them polymorphic. The mean similarity was 0.53, based on the Jaccard coefficient. The least similar individuals were FR5 and FL8; FR5 and FL9; FR1 and FR5. The most similar pairs of individuals were FL3 and FL4; FL4 and FL5. The UPGMA analysis clustered the individuals into two groups. Although no specific molecular marking that characterizes fruit coloration has been found, there is genetic variability among the evaluated individuals.

A mangabeira (Hancornia speciosa Gomes) e uma especie frutifera nativa do Brasil. A avaliacao de caracteristicas de crescimento se torna importante em pesquisas de melhoramento genetico e podem estar correlacionadas positivamente com produtividade. O presente trabalho objetivou avaliar aspectos iniciais de desenvolvimento em um teste de progenie, que possibilite fundamentos para conservacao da mangabeira. Foram avaliadas as variaveis: altura das mudas, diâmetro do coleto e sobrevivencia. O delineamento experimental utilizado foi em blocos casualizados, em que os tratamentos consistiram nos diferentes acessos utilizados na producao das mudas e nos tempos de avaliacao. Para comparacao do tempo de crescimento foi utilizado o teste de Tukey (p

A mangabeira (Hancornia speciosa Gomes – Apocynaceae) e uma planta nativa do Brasil, com crescente devastacao nas areas de ocorrencia natural. Estudos de diversidade genetica tem sido desenvolvidos para elaboracao de estrategias de conservacao da especie. Devido a demanda de pesquisadores interessados na busca de um protocolo padronizado para extracao de DNA de mangabeira, o presente trabalho foi desenvolvido para avaliar a eficiencia de protocolos de extracao de DNA na aplicacao de tecnicas moleculares em amostras da especie. Foram utilizadas folhas jovens provenientes de acessos do Banco Ativo de Germoplasma de Mangaba da Embrapa Tabuleiros Costeiros, localizado em Itaporanga d’Ajuda, Sergipe. Foram testadas quatro protocolos e os acidos nucleicos obtidos foram quantificados em espectrofotometro. Tres amostras com as maiores concentracoes de DNA foram utilizadas nos diferentes protocolos para a PCR-ISSR utilizando seis primers (UBC 864, UBC 848, UBC 835, UBC 817, UBC 811 e UBC 80). A analise conjunta da quantificacao e amplificacao da PCR sugere a recomendacao do protocolo de extracao 2 (PE2) para extracao de DNA de mangabeira.

The mangabeira (Hancornia speciosa G.) is a native Brazilian species in the process of domestication. It has several potential uses. In recent years the populations of mangabeira have become fragmented and, to conserve their genetic resources, Embrapa Tabuleiros Costeiros has, since 2006, maintained a Mangaba Genebank, which currently has 299 individuals distributed across 27 accessions. In one of these accessions (CP), first fruiting was observed to occur at 13 months, indicating precocity. The current project was developed with the intention of morphologically characterizing the CP accessions and evaluating the extent of genetic diversity between genotypes, based on genetic markers associated with fruiting precocity. Morphological variation in crown and leaf shape, growth habit, total height, stem diameter and canopy height were investigated. For molecular characterization, 18 CP accession genotypes were used, all from Sergipe State, Brazil, and 11 ISSR markers were studied that generated 62 fragments with 91.4% polymorphism. The fruits have physical and chemical characteristics suitable for in natura consumption and also for processing. UPGMA dendrogram analysis showed the genotypes CP2 and CP6 to be most similar and CP10 the most divergent within the CP accessions. The results showed the existence of polymorphism, and genetic divergence among genotypes, thus reinforcing the importance of constant germplasm characterization, which guarantees information on gene sources for future use, while providing information concerning potential parents that can be used in breeding programs as well as the identification of duplicates.

A abobora (Cucurbita moschata Duch) e uma olericola de grande aceitacao em todo o Brasil, especialmente na regiao Nordeste. E bastante utilizada na alimentacao diaria do nordestino, onde e conhecida por Jerimum, para elaboracao de diversos pratos e doces, que podem ser caseiros ou industrializados. Durante o processo de coccao, ha perda de nutrientes e alteracao na qualidade do alimento a ser consumido. O trabalho foi desenvolvido com o objetivo de avaliar as alteracoes ocorridas em aboboras crioulas apos a coccao. Os 115 frutos avaliados foram oriundos do programa de melhoramento de abobora da Embrapa Tabuleiros Costeiros (CPATC), colhidos em outubro de 2013, no Campo Experimental Pedro Arle, localizado no municipio de Frei Paulo, Sergipe. As analises foram realizadas na Sala de Pos-colheita do CPATC, em Aracaju. Os pedacos, pesando cerca de 200g foram fervidos em 1L de agua, onde permaneceram por cinco minutos. Antes e apos a coccao, todas as amostras foram avaliadas quanto ao peso da massa fresca, firmeza e teor de solidos soluveis (SS). Foi realizado o teste de Tukey para comparacao de medias, ao nivel de 5% de significância, utilizando-se o programa estatistico SAS. Nao ocorreu perda de massa fresca significativa, que variou de 142,28g (antes) a 138,27 g (apos a coccao). Entretanto, o teor de SS diminuiu significativamente de 11,68 para 11,08oBrix, e a firmeza, de 12,75 a 5,69N. As caracteristicas avaliadas neste trabalho, associadas a outras de importância nutricional e comercial, sao importantes informacoes ao programa de melhoramento da especie.

Banana (Musa spp) is a fruit species frequently cultivated and consumed worldwide. Molecular markers are important for estimating genetic diversity in germplasm and between genotypes in breeding programs. The objective of this study was to analyze the genetic diversity of 21 banana genotypes (FHIA 23, PA42-44, Maca, Pacovan Ken, Bucaneiro, YB42-47, Grand Naine, Tropical, FHIA 18, PA94-01, YB42-17, Enxerto, Japira, Pacova, Prata-Ana, Maravilha, PV79-34, Caipira, Princesa, Garantida, and Thap Maeo), by using inter-simple sequence repeat (ISSR) markers. Material was generated from the banana breeding program of Embrapa Cassava & Fruits and evaluated at Embrapa Coastal Tablelands. The 12 primers used in this study generated 97.5% polymorphism. Four clusters were identified among the different genotypes studied, and the sum of the first two principal components was 48.91%. From the Unweighted Pair Group Method using Arithmetic averages (UPGMA) dendrogram, it was possible to identify two main clusters and subclusters. Two genotypes (Garantida and Thap Maeo) remained isolated from the others, both in the UPGMA clustering and in the principal cordinate analysis (PCoA). Using ISSR markers, we could analyze the genetic diversity of the studied material and state that these markers were efficient at detecting sufficient polymorphism to estimate the genetic variability in banana genotypes.

Cambui (Myrciaria tenella O. Berg) is a native species from Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of genetic diversity is fundamental to the direction of the strategies necessary to form and maintain a germplasm. This study aimed to evaluate the genetic diversity in a natural population of cambui using inter-simple sequence repeat (ISSR) molecular markers. The natural population, which provided the plant material, is found at the Private Reserve of Natural Heritage of Caju, which belongs to the experimental field of Embrapa Tabuleiros Costeiros, in the municipality of Itaporanga d’Ajuda, SE, Brazil. Young leaves of each individual were collected for DNA extraction and analysis of PCR-ISSR. Thirty primers were tested and the top 10 were selected. The use of these primers resulted in 71 fragments with 98.3% polymorphism. Similarity of individuals ranged between 0.30 and 0.92. The most similar individuals were C13 and C17 and the most distant were C1 and C41. Through UPGMA, six distinct groups were identified. This information may be used for conservation of these genetic resources, germplasm exchange, creation of germplasm bank and in future studies with this species.

Mangaba (Hancornia speciosa Gomes) is found in areas of coastal tablelands in the Brazilian Northeast and Cerrado regions. This species has been subjected to habitat fragmentation that is mainly due to human activity, and requires conservation strategies. The aim of this study was to analyze the structure and inter- and intrapopulation genetic diversity of natural populations of H. speciosa Gomes using inter-simple sequence repeat (ISSR) molecular markers. A total of 155 individuals were sampled in 10 natural populations (ITA, PAC, IND, EST, BC, PIR, JAP, BG, NEO, and SANT) in the State of Sergipe, Brazil. Fifteen primers were used to generate 162 fragments with 100% polymorphism. Genetic analysis showed that the variability between populations (77%) was higher than within populations (23%). It was possible to identify five different groups by the unweighted pair group method with arithmetic mean and principal coordinate analysis, and only one individual (E10) remained isolated. Using ISSR markers it was possible to obtain a molecular profile of the populations evaluated, showing that these markers were effective and exhibited sufficient polymorphism to estimate the genetic variability of natural populations of H. speciosa Gomes.