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In this study, a rare strain of Escherichia coli co-harboring blaCTX-M-32 and blaCTX-M-2 genes was characterized by whole genome sequencing. Escherichia coli strain was recovered from a bovine cecal sample collected at slaughter. The strain was resistant to third and fourth generation cephalosporins, fluoroquinolones, aminoglycosides, tetracycline and sulfamethoxazole, which was in concordance with the genotype: blaCTX-M-32 and blaCTX-M-2, aac(3)-Via and tet(A) and sul1 genes, respectively. The E. coli strain was characterized as pathogenic, and belongs to the ST226 (CC226), serotype O141ab/ac:H5, phylogenetic group A and fumC27/fimH41 types. No virulence genes were found in the genome. According to the plasmid analysis, IncFIB, IncHI2 and p0111 were present. The analysis of the contigs revealed that the blaCTX-M-32 was located in the chromosome, upstream IS5-like/ISKpn26 and IS1380-like/ISEc9 sequences, whereas blaCTX-M-2 was in the p0111 plasmid, within an integron class 1, and flanked by Orf3/qacEΔ1 and IS91/ISCR1. To our knowledge, this is the first report of a multidrug resistant Escherichia coli strain co-harboring two CTX-M enzymes in a healthy bovine from Portugal. This finding allows a better understanding on the dissemination of these resistance mechanism among bacteria of animal origin.

Fruit enzymatic browning (EB) inhibition continues to be a challenge in the Food Industry. This physiological disorder results mainly from the oxidation of natural phenolic compounds by polyphenoloxidase (PPO) and peroxidase (POX) leading to the formation of brown pigments. EB can be controlled with the application of antioxidants, reducing/inhibiting the activity of these oxidative enzymes. In this study, strawberry tree (leaves and branches) and apple byproduct were the natural-based extracts (NES) selected, as potential tissue browning inhibitors, within a first screening of fifteen natural-based extracts with antioxidant properties. Phenolic profile, total phenolic content and antioxidant activity of the selected extracts were also performed as well as their depletion effect on the oxidative enzyme&rsquo, s activity and browning inhibiton in fresh-cut pears. Strawberry tree extracts (leaves and branches) revealed higher total phenolic content (207.97 &plusmn, 0.01 mg GAE.gNES&minus, 1 and 104.07 &plusmn, 16.38 mg GAE.gNES&minus, 1, respectively), confirmed by the plethora of phenolic compounds identified by LC-ESI-UHR-QqTOF-HRMS and quantified by HPLC. This phytochemical composition was reflected in the low IC50 against PPO and POX obtained. Despite the lower phenolic content (6.76 &plusmn, 0.11 mg GAE.gNES&minus, 1) and antioxidant activity (IC50 = 45.59 &plusmn, 1.34 mg mL&minus, 1), apple byproduct extract showed potential in delaying browning. This study highlights the opportunity of byproducts and agricultural wastes extracts as novel anti-browning agents.

Funding Information This study was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade–COMPETE and by Fundação para a Ciência e a Tecnologia (FCT, Portugal)—Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through funds to GHTM (UID/04413/2020), CIISA Project (UID/CVT/00276/2020), and Project PTDC/CVT-CVT/28469/2017. The contributions of Andrea T. Feßler and Stefan Schwarz were financially supported by the German Federal Ministry of Education and Research (BMBF) under project numbers 01KI1727D and 01KI2009D as part of the Research Network Zoonotic Infectious Diseases. Part of this research was supported by Cost Action CA18217: European Network for Optimization of Veterinary Antimicrobial Treatment (ENOVAT). Apramycin and florfenicol are two antimicrobial agents exclusively used in veterinary medicine. Resistance determinants to these antimicrobial agents have been described in several staphylococci, yet no inhibition zone-based epidemiological cutoff (ECOFF) values are available to detect populations harboring resistance mechanisms. In this study, we propose disk diffusion inhibition zone ECOFF values of Staphylococcus aureus for apramycin and florfenicol. The susceptibility to apramycin and florfenicol was evaluated by disk diffusion of five S. aureus collections, comprising 352 isolates of animal (n = 265) and human (n = 87) origin. The aggregated distributions of inhibition zone diameters were analyzed by the normalized resistance interpretation method to obtain normalized wild-type (WT) population distributions and corresponding ECOFF values. The putative WT populations of S. aureus were characterized by an inhibition zone ≥15 mm (ECOFF = 15 mm) for apramycin and ≥21 mm for florfenicol (ECOFF = 21 mm). Five nonwild-type (NWT) isolates were detected for apramycin, all without inhibition zone and harboring the apmA gene, whereas five NWT isolates were identified for florfenicol, all carrying the fexA gene. The proposed ECOFF values for apramycin and florfenicol may be a valuable tool in future antimicrobial resistance monitoring and surveillance studies to identify S. aureus NWT populations toward these antimicrobial agents. publishersversion published

The present study aimed to characterize the extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases (ESBL/PMAβ) among Escherichia coli producers isolated from beef, pork, and poultry meat collected at retail, in Portugal. A total of 638 meat samples were collected and inoculated on selective medium for the search of E. coli resistant to 3rd generation cephalosporins. Isolates were characterized by antimicrobial susceptibility testing, molecular assays targeting ESBL/AmpC, plasmid-mediated quinolone resistance (PMQR), and plasmid-mediated colistin resistance (PMCR) encoding genes. The highest frequency of E. coli non-wild type to 3rd generation cephalosporins and fluoroquinolones was observed in broiler meat (30.3% and 93.3%, respectively). Overall, a diversity of acquired resistance mechanisms, were detected: blaESBL [blaCTX-M-1 (n = 19), blaCTX-M-15 (n = 4), blaCTX-M-32 (n = 12), blaCTX-M-55 (n = 8), blaCTX-M-65 (n = 4), blaCTX-M-27 (n = 2), blaCTX-M-9 (n = 1), blaCTX-M-14 (n = 11), blaSHV-12 (n = 27), blaTEM-52 (n = 1)], blaPMAβ [blaCMY-2 (n = 8)], PMQR [qnrB (n = 27), qnrS (n = 21) and aac(6’)-Ib-type (n = 4)] and PMCR [mcr-1 (n = 8)]. Our study highlights that consumers may be exposed through the food chain to multidrug-resistant E. coli carrying diverse plasmid-mediated antimicrobial resistance genes, posing a great hazard to food safety and a public health risk.

Currently, cereal bars are gaining interest globally because of their nutritionally balanced and convenient nature. One healthy strategy is to add probiotics to cereal bars, to make them a functional food product. So, in this study a cereal bar functionalized with edible coatings of whey protein isolate (WPI) and alginate (ALG) incorporated with Bifidobacterium animalis subsp. lactis BB-12 and inulin was developed and evaluated for its consumer acceptability and physicochemical and microbiological properties, throughout 90 days of storage. WPI-coated cereal bars were shown to be the solution that better maintained the level of the incorporated probiotic strain when compared to the ones coated with ALG, throughout storage and throughout in vitro gastrointestinal digestion. The physicochemical properties of the bars, namely aw, moisture content, color and texture, were not altered during the storage period. The sensory evaluation showed that coated bars were accepted as well as control bars. Moreover, the consumers appreciated better the odor and flavor of WPI-coated bars than those of ALG-coated bars.

Storage techniques, such as low temperature and modified atmosphere packaging, are efficient in keeping visual quality of ready-to-eat (RTE) products such as processed leafy vegetables throughout the supply chain, but studies on appearance often neglect the effect of different storage conditions on their nutritional properties. The effects of initial O2 partial pressure (20, 10, 5 and 2.5 kPa O2) in packages and storage time (14 d) on RTE rocket leaves on the bioactive compounds, phenolics, ascorbic acid, and antioxidant activity were determined. Kinetic models were applied to allow the assessment and prediction of the influence of initial partial pressure on nutritional composition. The results show that lowering package initial O2 partial pressure from 20 kPa to 5–10 kPa reduced the respiration rate by 53 and 38%, respectively. However, antioxidant activity and ascorbic acid content showed most effects with an initial O2 of 5 kPa. The kinetic parameters are relevant to help predict phytochemical changes during storage under different package O2 partial pressures. To obtain an overall quality, the application of intermediate atmospheres would be beneficial.

We screened 1840 Enterobacteriaceae isolates from food-producing animals, meat, meat products and animal feed, for the detection of plasmid-mediated colistin resistance, during 2010-2015. The mcr-1 gene was detected in 8.0% (97/1206) Escherichia coli and in 0.47% (3/634) Salmonella enterica isolates, with a high number of mcr-1 positive E. coli isolates (45.7%) being extended-spectrum β-lactamase or plasmid-mediated AmpC β-lactamase co-producers. No mcr-2 gene was detected. Our findings highlight the spread of mcr-1 genes within a wide-ranging sample of food-producing animals and meat, in Portugal. Highlights: Spread of MCR-1 within a wide-ranging sample of food-producing animals and meat; Occurrence of mcr genes in E. coli isolates from turkeys, swine and broilers; High number of mcr positive E. coli isolates that were ESBL or PMAβ co-producers; First description of MCR-1-producing Salmonella Reading from bovine meat; New mcr-1.9 variant reported in an Escherichia coli from a swine. V. Manageiro was supported by Fundação para a Ciência e a Tecnologia (FCT) fellowship (grant SFRH/BPD/77486/2011), financed by the European Social Funds (COMPETE-FEDER) and national funds of the Portuguese Ministry of Education and Science (POPH-QREN). The authors also thank to FCT for project grant UID/MULTI/00211/2013. info:eu-repo/semantics/publishedVersion

Fresh-cut wild rocket (Diplotaxis tenuifolia L.) represents a very interesting ready-to eat (RTE) product with a high market value. Scarce information is available on product freshness and on quality attribute deterioration during storage. The aim of this work was to evaluate the respiration rate and functional quality of rocket salad when packaged under passive atmosphere composition in two different permeability bags. Wild rocket was packaged on two different sealed polypropylene bags: macroperforated (P) and a non-permeable (NP) film bag. Bags were stored at 4°C during 15 days and the O2 and CO2 concentrations inside the bags, the respiration rate, the functional quality (ascorbic acid, phenolic compounds, anthocyanins, and chlorophyll) and microbial load of RTE rocket were evaluated at five different time points. Results showed that NP packages generated a passive modified atmosphere, with continuous decrease of O2 concentration (close to anaerobiosis by 14 days) and with increasing CO2 levels up to ca. 8 kPa after 5 days. Rocket preserved in this type of bag showed higher respiration rate in the first 2 days, when compared with rocket packaged in P bags. The evolution of carbon dioxide and oxygen concentration in both systems did not exhibit significant impact in the ascorbic acid and chlorophyll content. A positive effect of oxygen availability inside P packages was observed on total phenolic compounds and total anthocyanin concentration of rocket leaves, but simultaneously higher microbial contamination by day 5 in comparison with NP packages.

Fresh-cut cantaloupe melon is valued for its aroma but is highly perishable. Temperature of storage (typically 0-5°C) is critical for maintaining fresh-cut melon quality, but often reaches 10 °C during transportation and in retail outlets. A comparison amongst 0, 5 and 10 °C storage temperatures for fresh-cut melon over 14 days reveals that storage at 0 °C is optimal for avoiding increases in microbial load and loss of vitamin C especially at later time points. However, higher temperatures maintain better the balance of esters (acetate versus non-acetate) and phenolic content. The whole volatile organic compound (VOC) profile can be used to discriminate both time and temperature effects especially at earlier time points. Potential VOC markers for changes in vitamin C from day 0 to day 6 of storage (3-methyl butane nitrile) and temperature (limonene) are identified through a multi-trait analysis.

Lysophosphatidylethanolamine (LPE) is a naturally occurring lipid with regulatory effects in senescence and ripening. When applied exogenously to horticultural crops, LPE affects growth, development, and postharvest longevity. The effects of exogenously applied LPE have been studied in a range of plant organs in more than a dozen horticultural species. The claimed horticultural benefits include delayed leaf senescence, stimulation of ripening in table grape, acceleration of color development and extension of shelf-life in cranberry and tomato, and increased vase life of cut flowers. Responses to LPE application are found to vary dramatically within horticultural commodity, developmental stage, and organ type. Effects on ethylene responses are contradictory. LPE inhibits phospholipase D and is reported to affect the activity of enzymes relevant for produce quality, such as phenylalanine ammonia lyase and acid invertase. The biochemical mode of action of LPE is poorly understood. In particular, a mechanism by which a plant growth regulator might delay senescence of plant organs and accelerate ripening-related changes is not obvious. The horticultural, physiological and biochemical effects of LPE are reviewed in an attempt to highlight the knowledge gaps regarding the putative regulatory role of exogenously applied LPE.

Effective and efficient design of modified atmosphere packaging (MAP) systems for ready-to-eat (RTE) products requires knowledge on produce respiration rate under various temperatures and oxygen levels. In order to model the respiratory behavior of RTE rocket leaves under different storage conditions, leaves respiration rate was measured during storage under four initial oxygen partial pressures (2.5; 5; 10 and 20 kPa O2) and three temperatures (0, 5 and 10 °C), for 10 days. The respiratory quotient was determined and found to range between 0.6 and 1.3. Temperature showed a significant impact on respiration rate and the effect of oxygen partial pressures was found to be temperature-dependent. The oxygen pressure effect on respiration rate was accurately described using Michaelis–Menten kinetics, without inhibition by carbon dioxide, and the effect of temperature was well modeled by exponential functions. Monte Carlo simulation allowed quantification of the relative impact of the reduction of oxygen pressure and temperature on the decrease in respiration rate and the potential shelf life increase of RTE rocket leaves. Temperature contributed to more than 80% of the Michaelis–Menten model variance while oxygen pressure contributed only with 13%. The model obtained is a useful tool for defining packaging and storage conditions for a shelf-life extension of rocket leaves.

Winter lettuce response to the application of composts from the solid fraction of dairy cattle slurry was assessed in combination with mineral nitrogen (N) fertilizer throughout a randomized block experiment under greenhouse conditions. Lettuce yield did not increase with mineral N fertilizer, possibly because the lettuce was preceded by a heavily fertilized tomato crop, and the amount of mineral N (114 kg ha−1) in the soil at the beginning of the experience was greater than the amount of N (64–89 kg ha−1) accumulated in the lettuce shoots of all of the treatments. In contrast, lettuce yield and N uptake increased with compost application, suggesting other benefits in addition to N availability resulting from its use as a soil amendment. Therefore, dairy cattle slurry solid fraction mature compost can be recommended for vegetable production, and mineral N recommendation is suggested to pursue previous soil mineral N analysis.

BACKGROUND: Orange-fleshed cantaloupe melons have intense aroma and flavor but are very perishable during storage life. Fresh-cut processing enhances ethylene-mediated quality losses. Post-cutting 1-methylcyclopene (1-MCP) application to fresh-cut cantaloupe was evaluated for its effects on quality attributes, phytochemical content and aroma volatiles. RESULTS: Fresh-cut cantaloupe (Cucumis melo var. cantalupensis ‘Fiesta’) cubes treated with 1.0 µL L−1 of 1-MCP for 24 h at 5 °C, packaged in vented plastic clamshells and stored under normal atmosphere at 5 °C for 9 days, preserved their soluble solids, total phenolics, total carotenoids and β-carotene contents, but significant softening occurred. A significant increase of non-acetate esters and a decrease of aldehydes occurred during storage. Most quality attributes of fresh-cut cantaloupe were unaffected by the treatment with 1-MCP. 1-MCP-treated fresh-cut cantaloupe accumulated higher levels of propyl acetate, 2-methylbutyl acetate, methyl butanoate, methyl 2-methyl butanoate, methyl hexanoate, 2-methylbutyl alcohol and phenethyl alcohol, and lower levels of benzyl alcohol and heptanal than untreated controls. CONCLUSION: Post-cutting treatment with 1-MCP affected nine of the flavor-important volatiles, particularly those derived from the amino acids isoleucine and phenylalanine, but had no practical effect on phytochemicals or other quality attributes. © 2012 Society of Chemical Industry

To improve fertilisation recommendations in organic farming systems and nitrate vulnerable zones, the response of white cabbage to the application of increasing rates of mineral N (0, 90 and 180 kg ha-1) fertiliser in combination with increasing rates (0, 20 and 40 t ha-1) of an organic fertiliser from the composting process (182 days) of the solid fraction (SF) of dairy cattle slurry, was assessed in the summer season in NW Portugal. Cabbage yield was strongly related to mineral N application with an N recovery rate over 70%, but was not associated to SF compost application, except for treatments without mineral N fertilisation where cabbage dry weight increased (P

Cabbage (B. oleracea var. capitata) response to 0 and 30 t/ha of mature cow and horse manure compost (C1: C/N=16.5) and of composted dairy cattle slurry solid fraction with different maturation periods and C/N ratios (C2: C/N=18.4; C3: C/N=21.8) and, incorporated into the soil one month before planting (B) and at planting (P), was evaluated throughout a randomized block design experiment. At the site, a mineral fertilized cabbage experiment was also carried out, with 180 kg ha-1 of mineral N. All crops were evaluated 0, 20, 34, 48 and 62 days after planting. The mineral fertilized cabbage showed increased growth rate compared to the organic crops with a final yield of 79.6 t/ha. The timing of compost application did not significantly affect crop growth, except for C2. Organic cabbage yield with the compost treatment C2B and compost C1 (mean of both application dates) were respectively 49.7 and 51.8 t/ha. Yield of crops grown with C2P and C3 were lower (mean of 36.9 t/ha) compared to C2B and C1 and were not significantly different from unfertilized crops (28.7 t/ha). Dry matter content was lower for mineral fertilized crops (9.2%) than for organic crops (mean 12.2%) and differences between the percentage of crop waste residues of organic (25.6%) and mineral fertilized (29.8%) crops were not statistically significant. The apparent compost N efficiency rate decreased from 13.7 to 11.2 and 9.2%, whereas N immobilization period increased from 37 to 42 and 45 days, respectively for C1, C2 and C3. Consequently, N uptake decreased from 99.7 (C1) to 89.8 (C2) and 79.5 kg/ha (C3). The N recovery rate for the mineral N application in the mineral fertilized cabbage crop was 55.3% (N uptake of 99.5 kg/ha). This study shows that as the C/N of the composts decreased, the period of N immobilisation decreased and the apparent compost N efficiency rate by the crop increased. Consequently, during the cabbage growing period more mineral N was available from the mineralisation of composts with a lower C/N ratio, as well as for the earlier compost application for the mature compost C2.

Cantaloupe and honeydew melon cultivars were processed and stored under a high oxygen passive modified atmosphere packaging (MAP) or reduced oxygen controlled atmosphere (CA; 5 kPa O2 + 10 kPa CO2 and balance N2) for 14 days at 5 °C. Atmosphere did not affect softening rate or soluble solids content and had a negligible effect on colour. Volatile compounds known as flavour-important in melons were extracted using stir bar sorptive extraction (SBSE) and quantified via GC-MS. Acetate esters increased more in MAP than in CA. Non-acetate esters increased markedly in both cultivars and storage types. Alcohols were more abundant in honeydew than in cantaloupe. Aldehydes decreased during storage in both cultivars and storage types. Lower O2 availability under CA conditions likely suppressed some of the esters relevant to the aroma of fresh-cut melon. Results suggest that package O2 levels are more important in determining aroma than other quality attributes of fresh-cut melon, and high O2 levels may be required to reveal desirable aroma compounds.

'Rocha' pear (Pyrus communis L.) was used as a model system to assess the effect of pH of dipping solutions on quality retention of fresh-cut fruit and its interaction with calcium additives. Pear slices were dipped for 60. s in a buffer solution at pH 3.0, 5.0 or 7.0 and stored at 4.5°C for 13 days. In other experiments, pear slices were dipped for 60. s in buffer solutions containing 250. mM of calcium ascorbate, lactate, chloride, and propionate, at pH 3.0 or 7.0, and stored at 4.5°C for 6 days. Browning and softening were more intense in slices dipped in a solution at pH 3.0 than at pH 5.0 or 7.0, but microbial growth was lower in slices treated at pH 3.0. The effect of calcium additives depended on the anion and significant interactions between the effects of calcium salt and pH were observed. Calcium ascorbate was very effective in preserving color and reducing microbial growth irrespective of pH, but enhanced pectin solubilization and tissue softening at pH 3.0. Slices treated with 250. mM calcium propionate or calcium lactate were softer and had higher electrolyte efflux when treated at pH 3.0 than at pH 7.0. Calcium lactate enhanced browning and reduced microbial growth at pH 3.0 but did not affect color or microbial counts at pH 7.0. All calcium treatments enhanced electrolyte leakage. pH of the dipping solution can affect, per se, the quality of fresh-cut fruit. The choice of calcium additives to prevent undesirable changes on visual and sensory quality of cut produce should involve pH ranges that provide the expected benefits.

Cantaloupensis melons are aromatic and fast senescing, whereas Inodorus have less odor and are slower senescing. Modified atmosphere packaging (MAP) is used to reduce respiration rate and extend shelf-life but may alter the synthesis of volatile compounds. We assessed the effects of passive MAP and controlled atmosphere (CA; 5 kPa O 2 + 10 kPa CO 2 ) upon quality and volatile production by fresh-cut 'Cantaloupe' and 'Honeydew' melons, stored at 5°C for 14 days. O 2 concentration inside the MAP packages decreased during the 14 days, to 11.1 and 14.2 kPa, in 'Cantaloupe' and 'Honeydew', respectively. CO 2 levels increased to 11.3 and 7.9 kPa during storage, for 'Cantaloupe' and 'Honeydew', respectively. In MAP, during the first 4 days of storage, cantaloupe cubes softened at a higher rate (2.0 N.day -1 ) than 'Honeydew' cubes (0.9 N.day -1 ). Conversely in CA, fresh-cut 'Honeydew' softened faster (1.9 N.day -1 ) than fresh-cut 'Cantaloupe' (0.9 N.day -1 ) which, softened faster between days 4 and 7 (2.1 N.day -1 ). Initial 'Cantaloupe' respiration was 17.5 ml CO 2 .kg -1 .h -1 , which increased to 63.8 and 43.4 ml CO 2 .kg -1 .h - 1 when stored in CA and MAP, respectively. Initial respiration of 'Honeydew' melon was 7.7 ml CO 2 .kg -1 .h -1 and increased to 55.5 and 30.0 ml CO 2 .kg -1 .h -1 when stored in CA and MAP, respectively. Volatile compounds that are known to be flavor-important in melons, were extracted using Stir Bar Sorptive Extraction (SBSE) and quantified via GC-MS. The production of acetate esters increased in both melons stored in MAP, but decreased in 'Cantaloupe' under CA conditions. Non-acetate esters increased in both cultivars and storage conditions. Alcohols, initially more abundant in 'Honeydew', underwent a decrease throughout storage. Aldehydes, initially more abundant in 'Cantaloupe', decreased in both melons for MAP but not in 'Honeydew' stored in CA. Lower O 2 availability in fresh-cut fruit, stored under CA conditions utilized in this experiment, likely suppressed some of the acetate esters relevant to the aroma of fresh-cut melon.

Second-order polynomial models were used to relate the independent variables, temperature, relative humidity and storage time, to the quality attributes (electrolyte leakage, chlorophylls, weight loss, humidity and headspace gas composition inside packages before and after packaging) of ready-to-eat spinach leaves. Results show that while waiting for processing the best conditions to assure high quality spinach leaves before packaging were 4 °C, 83 % RH and 4 h. The weight loss of spinach leaves was time-dependent: increasing the exposure time under these conditions decreased the amount of wet leaves before packaging. Under the same conditions, the electrolyte leakage was lower (4.63 %) and the chlorophyll content (45.44) was higher. After packaging and while waiting for dispatch, temperature most affected the package headspace atmosphere. Indeed, an increase in storage temperature from 3 to 7 °C led to a significant increase in respiration. The best combination of conditions in the high-care room was 3 °C, 74 % relative humidity for a maximum of 1 day before dispatch. In this case, the moisture content (0.46 %) and electrolyte leakage (4.16 %) were lower and the chlorophyll content (43.37) and oxygen concentration inside the packages of ready-to-eat spinach leaves (19.55 kPa) were higher.

A significant challenge for traditional food production is to improve its competitiveness by identifying innovations that guarantee the safety of the products, while at the same time meeting general consumer demands and specific consumer expectations towards traditional food (European Research on Traditional Food, EC, DG_Research, 2007). Therefore, there is a real need to study traditional foods in order to preserve important elements of European culture and, if possible, enrich and improve the diet across the whole continent. The following chapter will cover aspects related with the safety aspects of the traditional products including legislation, the hazards coming from raw materials as well as hazards from processing. Some technological solutions for making traditional foods safer in longer time will be presented. Even it is compulsory to follow the authentic recipe and technology; some small innovation would be necessary, for example, in packaging for better preservation of the traditional foods in order to meet consumer’s demands in having larger quantities of traditional foods. Technological innovations must be implemented in the companies always maintaining the traditional methodologies but assuring food safety and increasing shelf life of traditional foods.

The effect of calcium application and solution pH on ready-to-eat (RTE) baby-leaf spinach ( Spinacia oleracea L.) texture and structure preservation was studied. Spinach leaves were treated with calcium chloride, calcium lactate and calcium propionate, at two different pH conditions (5 and 7), packaged and stored for 7 days at 5 °C. After 24 h, the leaves crispness increased 49 and 29% for leaves treated with calcium chloride and lactate at pH 5 and the elasticity increased 100% after calcium propionate treatment at pH 7. During spinach shelf-life, the tissue flexibility decreased (20–60%) for all calcium treatments at pH 5 and 7 while tissue crispiness increased (7–40%) only for calcium treatments under pH 7. The electrolyte leakage increased throughout storage and was higher for all calcium treated samples when compared with controls. The chlorophyll content slightly decreased (12%) by the end of product shelf-life and did not differ according the calcium treatments. Total vitamin C content was lower in leaves treated with calcium chloride at pH 5 (31%) and pH 7 (19%) while the remaining treatments did not affect vitamin C content. The different calcium additives tested for potential texture quality maintenance did not provide the expected benefits on baby spinach leaves but increasing solution pH from 5 to 7 lead to an increased firmness by the end of shelf-life.

Rocket salad (Diplotaxis tenuifolia; wild rocket) is an important component of ready to eat salads providing a distinct peppery flavour and containing nutritionally relevant compounds. Quality deteriorates during post-harvest, in relation to time and storage temperature amongst other factors. Volatile organic compounds (VOCs) are easily measurable from rocket leaves and may provide useful quality indicators for e.g. changes in isothiocyanates derived from nutritionally important glucosinolates. VOC profiles discriminated storage temperatures (0, 5 and 10°C) and times (over 14days). More specifically, concentrations of aldehydes and isothiocyanates decreased with time paralleling a fall in vitamin C and a reduction in sensorial quality at the two higher temperatures. Sulphur containing compounds rise at later time-points and at higher temperatures coincident with an increase in microbial titre, mirroring a further drop in sensorial quality thus indicating their contribution to off-odours.

Molecular mobility is a fundamental parameter which reflects the dynamic properties of food components and contributes to food degradation reactions comprehension. Fresh-cut fruits have become an important food market segment. However, processing of fruits promotes faster its physiological deterioration, biochemical changes and microbial degradation. The purpose of this work was to use NMR methodology as a tool to evaluate fresh-cut fruit quality, during storage at refrigerated conditions. The fresh-cut melon transverse relaxation time (T2) was measured for a period of 7 days of storage at 5 °C. The relationship between the obtained values, microstructure and quality parameters was investigated. In general, results show the existence of one class of water fluidity in the system, the one present in cells after processing. T2, a measure of this fluidity, is affected by the processing and storage time. Also, it is possible to find a close relationships between T2 and quality parameters of total colour difference (TCD), firmness and aw. As T2 increases TCD also increases, while firmness and aw decrease. These results highlight the usefulness of NMR methodology application in food science., Author Joana F. Fundo acknowledges Fundacao para a Ciencia e a Tecnologia (grant SFRH/BD/62176/2009). The authors acknowledge the Portuguese NMR Network and Strategic Project PEst-C/CTM/LA0025/2013-14. This work was supported by National Funds from FCT through project PEst-OE/EQB/LA0016/2013

Melon ( Cucumis melo L.) is a widely consumed fruit whose major biologically active compounds are the vitamins C, pro-vitamin A, and folic acid, the phenolic phytochemicals and the toxic cucurbitacins. Levels of biologically active compounds in melon vary widely and are affected by genotype and environmental conditions during production and post-harvest handling, with strong interactions between cultivar and environmental effects. Ascorbic acid levels are generally reduced by processing methods. β-Carotene concentration also varies widely with genotype and is affected by processing conditions. Phenolics are generally preserved during storage and processing of raw materials but can be enhanced by roasting. Cucurbitacin levels, although negligible in commercial cultivars, may become important in pharmacological or ethnobotanical applications.

Wounding increases the hydrolysis of phospholipids mediated by phospholipase D (PLD) in plant tissues. Subsequent reactions may originate flavor-related aldehydes and alcohols. Lysophosphatidylethanolamine (LPE) is a naturally occurring phospholipid capable of inhibiting phospholipase D in vitro. The effect of LPE on the quality and volatile profile of fresh-cut melon was investigated. Cantaloupe melons (Cucumis melo L. 'Fiesta') were processed into cubes, vacuum-infiltrated with 200 μg L-1 of LPE, packaged in plastic clamshells and stored at 5°C for 9 days. The activities of phospholipase D (PLD) and C (PLC), respiration and ethylene production rates, color, firmness, soluble solids content (SSC), and volatile production were measured during storage at 5°C for 9 days. LPE significantly reduced the activity of PLD and PLC one day after the treatment but the effect was transient. LPE did not affect ethylene production and had a negligible effect on the respiration rate of melon cubes. Firmness and SSC were not affected by the treatment but LPE induced a very small increase in hue angle. LPE did not affect the major classes of volatiles in melon -esters and alcohols -but reduced the accumulation of aldehydes typical of wounding after the first day of storage. In conclusion, LPE did not help in the quality retention of fresh-cut melon but had a specific inhibitory effect on aldehyde production.

Orange-fleshed cantaloupe melons have intense aroma and flavor but are very perishable during storage life. Fresh-cut processing enhances ethylene-mediated quality losses. Post-cutting 1-methylcyclopene (1-MCP) application to fresh-cut cantaloupe was evaluated for its effects on quality attributes, phytochemical content and aroma volatiles.Fresh-cut cantaloupe (Cucumis melo var. cantalupensis 'Fiesta') cubes treated with 1.0 µL L(-1) of 1-MCP for 24 h at 5 °C, packaged in vented plastic clamshells and stored under normal atmosphere at 5 °C for 9 days, preserved their soluble solids, total phenolics, total carotenoids and β-carotene contents, but significant softening occurred. A significant increase of non-acetate esters and a decrease of aldehydes occurred during storage. Most quality attributes of fresh-cut cantaloupe were unaffected by the treatment with 1-MCP. 1-MCP-treated fresh-cut cantaloupe accumulated higher levels of propyl acetate, 2-methylbutyl acetate, methyl butanoate, methyl 2-methyl butanoate, methyl hexanoate, 2-methylbutyl alcohol and phenethyl alcohol, and lower levels of benzyl alcohol and heptanal than untreated controls.Post-cutting treatment with 1-MCP affected nine of the flavor-important volatiles, particularly those derived from the amino acids isoleucine and phenylalanine, but had no practical effect on phytochemicals or other quality attributes.

A fração sólida do chorume (FSC) de duas explorações de pecuária leiteira intensiva (FSC1 e FSC2, recolhidas em fossa aberta e fechada, respectivamente) foi compostada com e sem adição de palha, em pilhas estáticas e em pilhas com revolvimento. A temperatura aumentou rapidamente na FSC1, que foi recolhida mais lentamente, até a máxima diária de 68 °C, registrada na pilha estática com palha. No entanto, a FSC2 não alcançou temperatura superior a 51 °C em qualquer uma das pilhas. A mistura de palha com a FSC aumentou a temperatura no início da compostagem em todas as pilhas, mas as taxas de mineralização da matéria orgânica (MO) não aumentaram. Nas pilhas com revolvimento, a MO potencialmente mineralizável aumentou em comparação com as pilhas estáticas, mas a concentração de N nos compostos finais foi mais elevada nas pilhas sem revolvimento, sugerindo que o revolvimento pode ter aumentado as perdas de N. A relação C/N da FSC diminuiu de forma semelhante em todas as pilhas, desde um valor inicial de 27 ou superior até o valor de 11 a 13 no final da compostagem. A baixa temperatura, a baixa relação C/N e a baixa concentração de NH4+ em combinação com o aumento da concentração de NO3- dos compostos finais indicaram que estes estavam estabilizados. A elevada concentração de MO (720-820 g kg-1) e de N total (30-44 g kg-1) e a baixa condutividade elétrica (inferior a 100 mS m-1) sugerem que os compostos da FSC podem ser utilizados como corretivos orgânicos do solo com benefícios agronômicos e ambientais. No entanto, é necessário continuar a investigar para garantir a higienização do composto, porque a FSC2 não alcançou temperaturas termófilas durante a compostagem.

In the past two decades, zebrafish (Danio rerio)-based research has contributed to significant scientific advances. Still, husbandry and health programs did not evolve at the same pace, as evidenced by the absence of general guidelines. Health monitoring is essential to animal welfare, to permit animal exchanges across facilities, to contribute to robust experimental results, and for data reproducibility. In this study, we report a health program implemented in a zebrafish research facility to prevent, monitor, and control pathogen, and disease dissemination. This program includes quarantine, routine health screening of sentinels, and nonroutine screenings of retired animals and sick/moribund individuals. An extensive list of clinical signs, lesions, and pathogens was monitored based on: daily observation of fish, necropsy, histology, and bacterial culture. The results indicate that the combined analysis of sentinels with the evaluation of sick/moribund animals enables a comprehensive description not only of pathogen prevalence but also of clinical and histopathologic lesions of resident animals. The establishment of a quarantine program revealed to be effective in the reduction of Pseudoloma neurophilia frequency in the main aquaria room. Finally, characterization of the colony health status based on this multiapproach program shows a low prevalence of lesions and pathogens in the facility. Instituto Gulbenkian de Ciência (IGC); Animal House Core Facility (AHCF); info:eu-repo/semantics/publishedVersion