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With the introduction and implementation of core literacy, scientific thinking (ST) has become an essential goal and key dimension of science teaching. At present, there is no agreement on how to cultivate students' ST. This study took 238 sixth grade students from a public primary school in urban China as research sample, built a theoretical model of scientific thinking development based on the theory of the Bronfenbrenner' ecological systems theory, and used multiple data to explore and analyze the impact path of ST development of primary school students using the fuzzy sets of qualitative comparative analysis method (fsQCA). The development of urban primary school students' ST is the result of multiple factors at the level of individual drive, family environment, school teaching, and social resources. The result of data analysis showed that the influence path of ST development of urban primary school students driven by multiple factors includes three paths: parent participation leading, scientific practice leading, and home-school-community integration. We have interpreted the influence mechanism of each path in detail and put forward the enlightenment for science education policy and practice.

Purpose: The Program for the Education and Enrichment of Relational Skills (PEERS®) is a group-based social skills training program for adolescents on the autism spectrum. Although the program has been shown to be effective in improving social skills in autistic adolescents, evidence of its effectiveness from the Mandarin-speaking Chinese population is sparse. The present study used a non-randomized, pre- and post-intervention research design to investigate the feasibility and cultural validity of the program, as well as examine the moderators of intervention outcomes. Methods: Thirty-three autistic adolescents with intelligence quotient above 70 (M[subscript age] = 13.57, SD[subscript age] = 1.43; Male: Female 25:8) and their parents received 14 concurrent 90-minute sessions. Adolescents' autistic traits, challenging behaviors, emotional functioning, socio-cognitive process, social environment factors (school support), and caregivers' well-being were evaluated. Results: The findings suggest that with minor adjustments, the Mandarin version of PEERS® was generally acceptable and feasible for autistic adolescents and their parents. PEERS® may improve the social skills knowledge, reciprocal communication abilities, and emotional well-being of autistic adolescents. Also, participants with a higher level of school support, and parents with lower perceived subjective well-being at baseline may gain more benefits from PEERS®. The cultural adaptation and acceptability of the Mandarin Version of PEERS® were discussed. Conclusion: This feasibility study (Chinese Clinical Trial Registry: ChiCTR2200061417, 2022-06-23, retrospectively registered) provides a basis for further randomized control trials of the Mandarin version of PEERS®.

To study the protective effect of Rosa roxburghii Tratt compound plant beverage (RTCPB) against the acute alcoholic liver injury in mice, alcoholic liver injury models were established with 53% alcohol in male ICR mice using oral gavage. The drug was administered continuously for 21 days, the liver index, the content of triglyceride (TG), the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) contents were determined to evaluate the potential protective effect of RTCPB on acute alcohol-induced liver injury in mice. Meanwhile, the pathology analysis was performed on liver tissue to determine pathological changes. The results showed that the liver indices of mice in silymarin control and RTCPB groups were significantly decreased (P

AIM: To elucidate the neuropathological mechanisms underlying diabetic vitreous hemorrhage (DVH) and its correlation with clinical characteristics. METHODS: Twenty-one individuals with DVH (male/female 12/9; mean age 52.29±11.66y) were selected, alongside 21 appropriately matched controls with diabetes mellitus (DM). Voxel-based morphometry (VBM) techniques were employed to identify aberrant functional regions in the brain. Receiver operating characteristic (ROC) curves were utilized for classification based on the average VBM values of the two groups, and Pearson correlation analysis was conducted to assess the relationship between average VBM values in distinct brain regions and clinical manifestations. RESULTS: Relative to the DM controls, DVH patients exhibited reduced VBM values in the right superior temporal pole, the right superior temporal gyrus, the right medial orbital frontal gyrus, and the left superior frontal gyrus. Furthermore, ROC curve analysis of these four brain regions in DVH patients demonstrated a high degree of accuracy, as indicated by the area under the curve. The average VBM value in each of these regions exhibited a negative correlation with both the duration of DVH and the score on the Hospital Anxiety and Depression Scale (HADS). CONCLUSION: Pathological alterations in four distinct brain regions are observed in patients with DVH, potentially reflecting neuropathological changes associated with this condition.

AIM: To analyze whether alterations of voxel mirror homology connectivity (VMHC) values, as determined by resting-state functional magnetic resonance imaging (rs-fMRI), occur in cerebral regions of patients with hypertensive retinopathy (HR) and to determine the relationship between VMHC values and clinical characteristics in patients with HR. METHODS: Twenty-one patients with HR and 21 age-matched healthy controls (HCs) were assessed by rs-fMRI scanning. The functional connectivity between the hemispheres of the cerebrum was assessed by measuring VMHC, with the ability of VMHC to distinguish between the HR and HC groups assessed using receiver operating characteristic (ROC) curve analysis. Differences in the demographic and clinical characteristics of the HR and HC groups were analyzed by independent sample t-tests. The relationship between average VMHC in several brain areas of HR patients and clinical features was determined using Pearson correlation analysis. RESULTS: Mean VMHC values of the bilateral cuneus gyrus (BA19), bilateral middle orbitofrontal gyrus (BA47), bilateral middle temporal gyrus (BA39) and bilateral superior medial frontal gyrus (BA9) were lower in the HR than in the HC group. CONCLUSION: VMHC values can predict the development of early HR, prevent the transformation of hypertensive microangiopathy, and provide useful information explaining the changes in neural mechanism associated with HR.

Abstract Background The medical community has long been concerned about the cardiovascular disease risk in patients with type 2 diabetes. While liver fibrosis scores were originally designed for application in individuals with liver steatosis, an increasing number of studies have shown that they are also associated with cardiovascular disease risk. However, the association between Fibrosis-4 (Fib-4) in liver fibrosis scores and carotid atherosclerosis (CA) in patients with type 2 diabetes remains unclear. Objective The aim of this study is to investigate the association between the Fib-4 index and CA in patients with Type 2 diabetes. Additionally, it seeks to determine whether this relationship is influenced by factors including gender, age, body mass index (BMI), hypertension, and other variables. Methods Screening based on inclusion and exclusion criteria identified 2658 hospitalized patients with type 2 diabetes. Subsequently, patients were divided into three groups according to Fib-4 values (Fib-4 0.05). The results of the ROC curve analysis indicated that the AUC for Fib-4 predicting CA was 0.602 (P

Abstract Benign prostatic hyperplasia (BPH) is a prevalent urinary system disorder. Despite evidence of a significant genetic component from previous studies, the specific pathogenic genes and biological mechanisms are still largely unknown. The study utilized the FinnGen R10 dataset, encompassing 177,901 individuals (36,601 cases and 141,300 controls), and the GTEx v8 EQTLs files to conduct single-tissue and cross-tissue transcriptome-wide association studies (TWAS). FUSION method was utilized to validate the findings in specific tissues. Gene Analysis and Multi-marker Analysis of Genomic Annotation (MAGMA) were used to identify potential susceptibility genes. The intersection genes of the above results were analyzed by Mendelian randomization (MR), summary data-based MR (SMR) and colocalization studies. Fine-mapping of causal gene sets (FOCUS) software was employed to accurately locate risk genes. Gene Expression Omnibus (GEO) analysis explores the differential expression of genes. Finally, The GeneMANIA tool was utilized to further understand the functional roles of these susceptibility genes. The cross-tissue TWAS analysis revealed 28 genes associated with BPH susceptibility. Single-tissue TWAS and MAGMA further refined eight genes, and subsequent MR, SMR, FOCUS and colocalization analysis pinpointed INO80B as the key gene. The differential expression of this result was verified by GEO. INO80B may help prevent excessive prostatic cell proliferation by regulating cell cycle-related gene expression. Our research identified the INO80B gene, whose predicted expression is associated with BPH risk and hence provided a new insight into the genetic basis of this disease. However, further functional studies are necessary to elucidate the potential biological activity of these significant signals.

Abstract Direct resin restoration has been widely used to restore the tooth defect for its convenience and minimal invasion. However, the accuracy of the final contour of class II restoration is often unpredictable and quite technique-sensitive due to the limitation of traditional matrix band system. In this study, the authors developed a novel matrix band system using shape memory polymer (SMP), which possessed two reversible shapes including digitized permanent shape to reproduce anatomical proximal contour and programmable temporary shape for clinical practice and reservation. The permanent shape could be designed and customized in software or retrieved from imaging database collected from large general population. Only warm saline or water was needed to trigger the shape transformation without any auxiliary tools. The results of in vitro class II restoration showed the shape memory matrix bands brought the best proximal profile and contact point, compared to traditional metal matrix and sectional matrix. The novel shape memory matrix system represents the comfortable, precise, and minimally invasive trend of dentistry and has a broad industrialization prospect as a dental instrument.

Abstract One of the key advantages of perovskite light-emitting diodes (PeLEDs) is their potential to achieve high performance at much higher current densities compared to conventional solution-processed emitters. However, state-of-the-art PeLEDs have not yet reached this potential, often suffering from severe current-efficiency roll-off under intensive electrical excitations. Here, we demonstrate bright PeLEDs, with a peak radiance of 2409 W sr−1 m−2 and negligible current-efficiency roll-off, maintaining high external quantum efficiency over 20% even at current densities as high as 2270 mA cm−2. This significant improvement is achieved through the incorporation of electron-withdrawing trifluoroacetate anions into three-dimensional perovskite emitters, resulting in retarded Auger recombination due to a decoupled electron-hole wavefunction. Trifluoroacetate anions can additionally alter the crystallization dynamics and inhibit halide migration, facilitating charge injection balance and improving the tolerance of perovskites under high voltages. Our findings shed light on a promising future for perovskite emitters in high-power light-emitting applications, including laser diodes.

Abstract Aerial manipulators can manipulate objects while flying, allowing them to perform tasks in dangerous or inaccessible areas. Advanced aerial manipulation systems are often based on rigid-link mechanisms, but the balance between dexterity and payload capacity limits their broader application. Combining unmanned aerial vehicles with continuum manipulators emerges as a solution to this trade-off, but these systems face challenges with large actuation systems and unstable control. To address these challenges, we propose Aerial Elephant Trunk, an aerial continuum manipulator inspired by the elephant trunk, featuring a small-scale quadrotor and a dexterous, compliant tendon-driven continuum arm for versatile operation in both indoor and outdoor settings. We develop state estimation for the quadrotor using an Extended Kalman Filter, shape estimation for the continuum arm based on piecewise constant curvature, and whole-body motion planning using minimum jerk principles. Through comprehensive fundamental verifications, we demonstrate that our system can adapt to various constrained environments, such as navigating through narrow holes, tubes, or crevices, and can handle a range of objects, including slender, deformable, irregular, or heavy items. Our system can potentially be deployed in challenging conditions, such as pipeline maintenance or electricity line inspection at high altitudes.

Abstract Background Acute and critical neurological diseases are often accompanied with elevated intracranial pressure (ICP), leading to insufficient cerebral perfusion, which may cause severe secondary lesion. Existing ICP monitoring techniques often fail to effectively meet the demand for real-time noninvasive ICP monitoring and warning. This study aimed to explore the use of electrical impedance tomography (EIT) to provide real-time early warning of elevated ICP by observing cerebral perfusion. Methods An intracranial hypertension model was prepared by injecting autologous un-anticoagulated blood into the brain parenchyma of twelve Landrace swine. Invasive ICP monitoring was used as a control method, and a high-precision EIT system was used to acquire and analyze the changing patterns of cerebral perfusion EIT image parameters with respect to ICP. Four EIT parameters related to cerebral perfusion were extracted from the images, and their potential application in detecting ICP elevation was analyzed. Results When ICP increased, all EIT perfusion parameters decreased significantly (P 22 mmHg), the correlation between EIT perfusion parameters and ICP was more significant (P

Abstract The spatial role of M1 and M2 tumor-associated macrophages (M1/M2 TAMs) in precision medicine remains unclear. EGFR and TP53 are among the most frequently mutated genes in lung adenocarcinoma. We characterized the mutation status and density of M1/M2 TAMs within tumor islets and stroma in 117 lung adenocarcinomas using next-generation sequencing and immunohistochemistry, respectively. Stromal M1 TAMs were positively correlated with disease progression and smoking history. In contrast, islet M1/M2 TAMs were predominantly found in tumors with wild-type TP53 (wtp53) but not associated with EGFR status. The presence of wtp53 was associated with the spatial distribution of M1/M2 TAMs in tumor islets and stroma. Additionally, dominance of islet M1 TAMs and M1-signature were significantly associated with improved survival in patients with wtp53 lung adenocarcinoma, unlike in those with mutant TP53. Conditioned medium from M1 macrophages (M1 CM) induced apoptosis in wtp53 cells through increased p53 accumulation. We found that interferons in M1 CM activate JAK1/TYK2 via IFNARs, leading to enhanced STAT1 expression and Y701 phosphorylation. This activation facilitates p53-STAT1 interactions, reduces the interaction between p53 and MDM2, and subsequently decreases p53 ubiquitination. M1 CM inhibited tumorigenesis, and silencing p53 reduced the anti-tumor efficacy of polyinosinic:polycytidylic acid (poly I:C) in vivo. Furthermore, higher M1-signature was significantly associated with better responses and survival following anti-PD1 treatment in wtp53 melanomas. IFNs/STAT1/p53 signaling was critical for the anti-tumor activity of M1 macrophages. These findings suggest that p53 modulates the spatial balance of M1/M2 TAMs, and the tumoricidal effects of M1 TAMs depend on p53 status. Thus, p53 companion diagnostics could facilitate the development of M1-oriented therapies, which may be particularly beneficial for wtp53 patients when combined with immunotherapy.

Abstract Background The coronavirus disease 2019 (COVID-19) pandemic placed a heavy strain on the United States healthcare system. Common hospital operational performances were impacted to varying degrees by the pandemic. This study examined the healthcare operational measures during COVID-19 pandemic. Methods This cross-sectional study examined the COVID-19 cases and death counts of 56 California counties and hospital-level utilization data of 397 hospitals in California from January 2019 to March 2021. Results A total of 56 California counties were analysed, of which 37 counties were urban and 19 counties were rural. Average patient length of stay was positively associated with the number of intensive care unit visits by COVID-19 patients for all counties, as well as urban and rural counties separately. However, average patient length of stay was negatively associated with the number of inpatient visits by COVID-19 patients for all counties and urban counties. Conclusions The findings suggest that, while the need for additional beds and nursing staff in intensive care units exceeded initial estimates, there were also opportunities to streamline the care process for improved efficiency in regular acute care units. The understanding of factors impacting average patient length of stay would be valuable for hospital administrators in optimizing resource allocation and utilization to balance patient outcomes with financial sustainability during disruptive events.

Abstract Amino acids are important bio-based products with a multi-billion-dollar market. The development of efficient high-throughput screening technologies utilizing biosensors is essential for the rapid identification of high-performance amino acid producers. However, there remains a pressing need for biosensors that specifically target certain critical amino acids, such as l-threonine and l-proline. In this study, a novel transcriptional regulator-based biosensor for l-threonine and l-proline was successfully developed, inspired by our new finding that SerE can export l-proline in addition to the previously known l-threonine and l-serine. Through directed evolution of SerR (the corresponding transcriptional regulator of SerE), the mutant SerRF104I which can recognize both l-threonine and l-proline as effectors and effectively distinguish strains with varying production levels was identified. Subsequently, the SerRF104I-based biosensor was employed for high-throughput screening of the superior enzyme mutants of l-homoserine dehydrogenase and γ-glutamyl kinase, which are critical enzymes in the biosynthesis of l-threonine and l-proline, respectively. A total of 25 and 13 novel mutants that increased the titers of l-threonine and l-proline by over 10% were successfully identified. Notably, six of the newly identified mutants exhibited similarities to the most effective mutants reported to date, indicating the promising application potential of the SerRF104I-based biosensor. This study illustrates an effective strategy for the development of transcriptional regulator-based biosensors for amino acids and other chemical compounds.

Abstract We present a novel approach to realize three-dimensional (3D) matter wave solitons (MWSs) transformation between different optical potential wells by manipulating their depths and centers. The 3D MWSs are obtained by the square operator method, and transformed to other types (elliptical/ring/necklace) by performing time evolution with the split-step Fourier method. The effectiveness and reliability of our approach is demonstrated by comparing the transformed solitons with those obtained iteratively using the square operator method. Redistribution of MWSs are observed due to the modulation of potentials. In certain complex optical potential wells, we demonstrate the generation of exotic MWSs, such as double gyration modes, through this transformation approach. Overall, the controllable soliton transformation provides an excellent opportunity for all-optical switching, optical information processing, and various other applications.

Abstract Separation of multi-component mixtures in an energy-efficient manner has important practical impact in chemical industry but is highly challenging. Especially, targeted simultaneous removal of multiple impurities to purify the desired product in one-step separation process is an extremely difficult task. We introduced a pore integration strategy of modularizing ordered pore structures with specific functions for on-demand assembly to deal with complex multi-component separation systems, which are unattainable by each individual pore. As a proof of concept, two ultramicroporous nanocrystals (one for C2H2-selective and the other for CO2-selective) as the shell pores were respectively grown on a C2H6-selective ordered porous material as the core pore. Both of the respective pore-integrated materials show excellent one-step ethylene production performance in dynamic breakthrough separation experiments of C2H2/C2H4/C2H6 and CO2/C2H4/C2H6 gas mixture, and even better than that from traditional tandem-packing processes originated from the optimized mass/heat transfer. Thermodynamic and dynamic simulation results explained that the pre-designed pore modules can perform specific target functions independently in the pore-integrated materials.