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The predatory mirid Macrolophus praeclarus is widely distributed throughout the Americas, and is reported to prey upon several horticultural pest species. However, little is known about its biology, thermal requirements, crop odour preferences, phytophagy, and capability to induce defensive responses in plants. When five temperatures studied (20, 25, 30, 33 and 35°C) were tested and Ephestia kuehniella was used as prey, the developmental time from egg to adult on tomato, was longest at 20°C (56.3 d) and shortest at 33°C (22.7 d). The ability of nymphs to develop to adults decreased as the temperature increased, with the highest number of nymphs reaching the adult stage at 20°C (78.0%) and lowest at 35°C (0%). The lower and upper developmental thresholds were estimated at 11.2° and 35.3°C, respectively. The maximum developmental rate occurred at 31.7°C and the thermal constant was 454.0 ± 8.1 degree days. The highest predation rate of E. kuehniella eggs was obtained at 30°C. In Y-tube olfactory choice tests, M. praeclarus selected tomato, sweet pepper and eggplant odours more frequently than no plant control treatment. Macrolophus praeclarus feeding did not damage tomato plants compared to another zoophytophagous mirid, Nesidiocoris tenuis, which caused necrotic rings. The phytophagy of M. praeclarus induced defensive responses in tomato plants through the upregulation of the jasmonic acid metabolic pathway. The implications of the findings for using M. praeclarus in tomato biological control programmes in the Americas are discussed.

Fiorinia phantasma Cockerell & Robinson is an invasive, polyphagous pest recently found causing frond losses in ornamental palms in Miami-Dade County and in nurseries in Palm Beach County, Florida, USA. In this article, we document the discovery of this pest, its associated damage, closely related species, potential host plant species, a field guide, basic diagnostics, field dispersal, and available management options. Fiorinia phantasma is a pest of concern for ornamental plants demanding quick development of its management in Florida and preventative measures for other states in the United States. This article will help in detecting, identifying, and managing its early infestations.

Made available in DSpace on 2020-12-12T02:10:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) U.S. Department of Agriculture Citrus leprosis is a serious viral disease of citrus transmitted by Brevipalpus mites. The disease is present in South America, Central America, and Mexico, but not in the United States. One of its primary vectors, however, is present in Florida and other southern states. Non-viruliferous Brevipalpus yothersi (Acari: Tenuipalpidae) are present in Florida and could facilitate the spread of the disease if citrus leprosis viruses (CiLVs) are introduced into the state. In preparation for the possible incursion of CiLVs, we evaluated the biological control potential of four predatory mite species (Neoseiulus longispinosus, N. californicus, Amblyseius largoensis; Acari: Phytoseiidae) and Hemicheyletia bakeri (Acari: Cheyletidae) that are naturally associated with Brevipalpus spp. in citrus. In laboratory no-choice assays, the phytoseiid mites preyed mostly upon immature stages of B. yothersi, while H. bakeri preyed mainly on adults. In dual choice assays, all predators preferred Tetranychus urticae (Acari: Tetranychidae) over B. yothersi, but A. largoensis consumed more B. yothersi than the other three predators. A greenhouse experiment was performed to evaluate the potential of A. largoensis and H. bakeri to suppress B. yothersi populations on citrus trees. Four predator release treatments consisting of single or combined releases of A. largoensis and H. bakeri at a 1:10 (predator: B. yothersi) ratio were tested. Releases of A. largoensis alone or in combination with H. bakeri effectively suppressed B. yothersi and reduced damage to citrus plants. Release of H. bakeri alone, however, had no suppressive effect on B. yothersi populations. Overall, our results show that A. largoensis could be an efficient biological control agent of B. yothersi and could play an important role in citrus IPM programs tailored to manage citrus leprosis. Tropical Research & Education Center University of Florida, 18905 SW 280th Street Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP) Departamento de Fitossanidade Çukurova University Agricultural Faculty Department of Plant Protection Acarology Lab United States Department of Agriculture Animal and Plant Health Inspection Service Plant Protection and Quarantine Science and Technology Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP) Departamento de Fitossanidade

Miridae (Hemiptera) of the tribe Dicyphini are important zoophytophagous predators use to control pest arthropods in vegetable crops. However, the risk that their herbivory may cause economic damage could hinder their application as useful biocontrol agents and may limit the likelihood they would meet regulatory requirements for importation. We conducted field cage studies to assess the predation capacity and tomato plant damage of three mirid species established in south USA, a known biocontrol agent (Nesidiocoris tenuis), and two native species (Macrolophus praeclarus and Engytatus modestus). All three species significantly reduced the number of whiteflies (Bemisia tabaci) on tomato plants compared to tomato plants without mirids. More damage, evaluated as the number of necrotic rings, was observed on tomato plants with E. modestus and N. tenuis compared to M. praeclarus. In our experiments that included sesame plants (Sesamum indicum) with tomato plants, mirid numbers increased despite a low number of prey, thus showing a benefit of the plant-feeding habit of these predators. USA&rsquo, s established mirids may therefore prove to be immediately available biological agents for the management of present and future tomato pests.

Synthetic chemical lures mimicking pheromones or food attractants are essential tools in eradication programs for invasive species. However, their uses in programs aiming to control or eradicate terrestrial gastropods are largely unexplored. The goal of this study was to find a synthetic attractant that could aid in the eradication or management of the giant African snail (Lissachatina fulica). Field studies in Hawaii showed that a commercial papaya-flavored oil attracted snails. Analysis of the odor profile of the oil identified a total of 22 chemicals, which comprised > 98% of the volatile compounds emitted by the oil. A synthetic blend was reconstructed that mirrored the release rates of the papaya oil odors. In laboratory and field bioassays, the reconstructed blend, applied to cotton wicks as water and canola oil or water and mineral emulsions, attracted more snails than the water and oil emulsion control wicks. Field studies in Hawaii and Florida showed that the reconstructed blend in an oil emulsion was not attractive to non-target species such as butterflies or bees. The snails were attracted from distances > 1 m and entered traps baited with the attractant emulsion. When tested in the South Florida giant African snail eradication program, direct ground application of the reconstructed papaya-flavored oil emulsion increased the number of snails killed by over 87% compared to water emulsion controls. Integrating tactics using the synthetic papaya oil attractant into control measures should increase the effectiveness of eradication and management programs.

In pest eradication programs, traps can directly reduce pest populations; however, their application to gastropod programs remains relatively unexplored. The South Florida Giant African Snail, Liassachatina fulica (Pulmonata: Achatinidae), eradication program allowed a realistic evaluation of their utility. Field studies were conducted to determine the best bait, barrier and trap for use during the eradication program. Immature and adult snails were attracted to banana fruit and a commercially produced bait but only the commercially produced bait did not attract non-target and pest mammals. Four commercially produced traps and 4 barriers were field evaluated for snail retention efficacy. Snails escaped all traps and trap/barrier combinations but the rate of escape ranged from 10-100% after 24 hrs. Laboratory studies confirmed that snails can survive crossing a 5 cm barrier of copper tape, salt, insect stickem or antifouling paint. In the laboratory study snails did not cross copper sulfate but they crossed the barrier in the field. Adding salt to traps as a means to retain snails reduced the number of snails trapped. Laboratory studies confirmed that dry salt decreased the number of snails entering traps and snails did not enter traps when the salt was dissolved in water. Two trap types and the commercial bait were selected for a large-scale program test. For three months, trapping along with hand collection and pesticide application were conducted on 114 properties in five locations. Traps caught snails when surveys and regular pesticide applications on the same properties did not detect them. On 21 occasions snails were only found in traps, and both immature and adult snails were caught. This study showed that traps could be effectively deployed in an eradication program and they could capture snails that may have escaped other control measures.

The redbay ambrosia beetle, Xyleborus glabratus Eichhoff (Coleoptera: Curculionidae: Scolytinae), due to its association with Raffaelea lauricola T.C. Harr., Fraedrich & Aghayeva (Ophiostomatales: Ophiostomataceae), a pathogen that causes laurel wilt, is considered one of the most damaging pests of Persea species (Laurales: Lauraceae) including avocado. Currently, there is no satisfactory method to control this pest. Biological control is being examined as an additional tool to be used to lower the pest population and slow its spread. The objective of this study was to determine the natural enemy community associated with X. glabratus in Florida and Taiwan by using 3 methods: 1) field-collected wood naturally infested with X. glabratus (Florida), 2) bolts of avocado artificially infested with X. glabratus (Florida) and 3) direct collection of natural enemies from a trap area baited with infested wood and known X. glabratus lures (Florida and Taiwan). Among the predacious insects, there were 8 species of Laemophloeidae, an unidentified species of Staphylinidae, Microsicus spp. (Zopheridae), and Europs sp. (Monotomidae) (all Coleoptera). Among the parasitoids, hymenopterans of the families Braconidae, Eulophidae, Pteromalidae, Encyrtidae, Eupelmidae, and Bethylidae emerged from wood containing various species of Scolytinae. However, the only specimens that emerged from logs in which X. glabratus was present were Bethylidae, Braconidae, Encyrtidae (perhaps Closterocerus sp.), and Scelionidae. Four hymenopteran species were collected using attractants in Florida and Taiwan. However, more studies are needed to clarify their role as natural enemies of X. glabratus.

Summary Field surveys revealed that in south Florida the recently established Mikania micrantha Kunth (Asterales: Asteraceae: Eupatorieae) had a lower diversity of monophagous insect herbivores compared to the native Mikania spp. or Chromolaena odorata (L.) R. M. King & H. Rob. (Asterales: Asteraceae: Eupatorieae). In addition, Mikania micrantha served as a host for pests of agricultural and ornamental crops in Florida.

Attraction of the invasive snail Xerolenta obvia (Menke, 1828) to chopped cucumber and to a previously identified attractant based on a steam distillate of dry cat food was assessed with an established field population in Montana. Snails were significantly more attracted to the chopped cucumber than bottled water (control), using both traps and direct observations of attractants deployed in Petri dishes. The chopped cucumber also attracted significantly more snails to traps than the cat food distillate. These results suggest that chopped cucumber could constitute a cheap and simple lure for this and other invasive mollusk species. In addition, odors of chopped cucumber may support development of a new artificial lure, through analysis and reconstruction of the odor with synthetic compounds.

Tropical soda apple (Solanum viarum Dunal) (Solanaceae) is a small shrub native to South America that is invasive in pastures and conservation areas across Florida. Dense patches of tropical soda apple not only reduce cattle stocking rates and limit their movement, but also serve as reservoirs for pests of solanaceous crops. A classical biological control program was initiated in 1994 with exploration for natural enemies of tropical soda apple in Argentina, Brazil, and Paraguay. Host specificity tests conducted under laboratory and field conditions demonstrated that the leaf feeding beetle Gratiana boliviana Dunal (Coleoptera: Chrysomelidae) was a specialist herbivore that completes development only on the target weed. After obtaining appropriate permits, field releases of G. boliviana were initiated in Florida in May of 2003. Larvae and adults of G. boliviana feed on tropical soda apple leaves and may completely defoliate their host plants, resulting in reduced growth and fruit production. Mass rearing facilities for the beetle were established in northern, central and southern Florida, and adults were either hand-carried or transported to release sites by overnight courier. From 2003 to 2011, a total of 250,723 beetles were released and they became established throughout Florida, however, their impact is more noticeable in regions below latitude 29 °N. Reductions of tropical soda apple densities caused by damage by the beetle were visible 2-3 yr after initial release, or in some cases, within a few months. Various methods of technology transfer were used to inform the public, land owners, funding agencies and scientists about the biological control program, including articles in trade magazines, extension publications, websites, videos, field days and scientific publications. The project was successful because of the coordinated efforts of personnel from federal, state and county agencies.

Early detection of potentially invasive pests is critical to avert significant economic and environmental damage that may result from their successful introduction and establishment in the U.S. Recent advances in affordable USB (universal serial bus) compliant digital microscope cameras and internet platforms for disseminating information in real time have created the potential for enhanced training for insect pest identification. Using the palm weevil genus Rhynchophorus as a test group, we conducted real time training demonstrations which suggested that remote identification training is possible with the U.S. government internet-based portal “FoodShield” which employs Adobe Connect software, along with an open conference call line to reduce audio feedback. A training module was developed employing easy to use keys with photographs of diagnostic characters for species of Rhynchophorus that were distributed with an observation kit (containing image capture software, a digital microscope, a stand,...

A pheromone-based system to locate and monitor Planococcus minor (Maskell), a pest of over 250 plants including citrus, grape, and cacao, was tested. The difficulty in distinguishing P. minor from the citrus mealybug, P. citri, makes finding and evaluating the impact of the pest challenging. Studies conducted in Puerto Rico determined that synthetic P. minor pheromone lures preaged 120 d in the field caught similar number of males as lures not aged (fresh). Molecular analysis of trapped mealybug males using mitochondrial cytochrome oxidase-1, the internal transcriber space two locus, and 28S-D2 gene showed the pheromone traps to be species specific. Traps baited with P. minor pheromone were used to monitor the pest in south Florida and to locate potential infestations. P. minor males were found at all locations studied in South Florida and were present in low numbers (1.03 ± 0.69 mean ± SE/trap/14 d). Over 14,000 terminals, fruit, and flowers were visually inspected over a 6 mo period of peak tra...

The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae), ranks among the most important pests of various palm species. The pest originates from South and Southeast Asia, but has expanded its range dramatically since the 1980s. We used ecological niche modeling (ENM) approaches to explore its likely geographic potential. Two techniques, the Genetic Algorithm for Rule-set Prediction (GARP) and a maximum entropy approach (MaxEnt), were used. However, MaxEnt provided more significant results, with all 5 random replicate subsamples having P < 0.002 while GARP models failed to achieve statistical significance in 3 of 5 cases, in which predictions achieved probabilities of 0.07 < P < 0.10. The MaxEnt models predicted successfully the known distribution, including the single North American occurrence point of Laguna Beach, California, and various areas where the pest has been reported in North Africa, southern Europe, Middle East and South and Southeastern Asia. In addi...

Effect of temperature on the developmental and reproductive biology of the passionvine mealybug, Planococcus minor (Maskell) (Hemiptera: Pseudococcidae), was investigated on sprouted potatoes. P. minor was able to develop and complete its life cycle at 20, 25, and 29 °C. No eggs eclosed at 15 and 35 °C. The developmental time from egg to adult female was approximately 49 d at 20 °C, 31 d at 25 °C, and 27 d at 29 °C. Between 20 and 29 °C, 58-71% of eggs survived to adulthood. Female mealybugs made up 60-73% of the adult populations in the 3 temperature treatments. Preoviposition and oviposition times decreased as the temperature increased. Females reproduced sexually and produced the highest number of eggs (270 eggs/female) at 20 °C. Adult female longevity declined from 34 d at 20 °C to 19-22 d at the 2 higher temperatures. Adult males were short-lived and their longevity declined with increasing temperature. At 25 °C, the gross reproductive rate (GRR) and net reproductive rate (Ro) were estimated at 445.7 ♀/♀ and 325.4 ♀/♀, respectively, the generation time (TG) was 39.5 d, the intrinsic rate of increase (rm) was 0.147 (♀/♀/d), the finite rate of increase (λ) was 1.158 (♀/♀/d), and the doubling time (DT) was 4.7 d. The ability of P. minor to develop, survive, and reproduce successfully from 20 to 29 °C suggests that the mealybug has the potential to develop and establish in climatic zones that fall within this temperature range. El efecto de la temperatura sobre el desarrollo y la reproduccion de Planococcus minor (Maskell) (Hemiptera: Pseudococcidae) fue investigado en papas germinadas. Planococcus minor pudo completar su ciclo de vida a 20, 25 y 29 °C. Los huevos no eclosionaron a 15 y 35 °C. El tiempo de desarrollo de huevo a adulto hembra fue de aproximadamente 49 dias a 20 °C, 31 d a 25 °C, y 27 d a 29 °C. Entre 20 y 29 °C el 58-71% de los huevos sobrevivieron y alcanzaron la adultez. El porcentaje de hembras fue de 60 al 73% en las tres temperaturas. Los periodos de preoviposicion y oviposicion, y la longevidad de los adultos, aumentaron con la disminucion de la temperatura. Las hembras se reproducen sexualmente. La fecundidad mas alta fue a los 20 °C cuando cada hembra produjo un promedio de 270 huevos. La longevidad de las hembras se redujo de 34 d a 20 °C a 19 -22 dias a las dos temperaturas mas altas. Los machos viven poco tiempo y su longevidad se reduce con incrementos en la temperatura. A los 25 °C, la tasa neta de reproduccion (Ro) fue 325.4 ♀/♀, el tiempo de generacion (TG) 39.5 dias, la tasa intrinseca de crecimiento (rm) 0.147 (♀/♀/d), la tasa finita de incremento (λ) 1.158, y el tiempo de duplicacion (TD) fue de 4.7 dias. La capacidad de P. minor para completar su desarrollo, sobrevivir y reproducirse con exito de los 20 a los 29 °C sugiere que esta cochinilla tiene potencial para establecerse en zonas climaticas con este mismo rango de temperatura. View this article in BioOne

Planococcus minor (Maskell) is native to South Asia, but it is also present in several Neotropical locations including the island of Trinidad in the southern Caribbean. The mealybug poses a serious threat to unin- fested countries in this region as well as the mainland U.S. As part of an effort to gather much needed information on P. minor, 33 cocoa (Theobroma cacao L.) field sites on the island were surveyed in 2006 with a view to assess the occurrence and pest status of the mealybug. P. minor was identified from 20 field sites, indicating that it was well distributed across the island on this crop, which appeared to be a reliable indicator host plant. Infestation levels were generally low and populations were sparsely dis- tributed across the field sites categorized into three habitat types. The following year, nine field sites were surveyed for natural enemies of P. minor using laboratory-infested potatoes in sentinel traps. Spe- cies from four insect orders and six families were collected and identified. The major predators belonged to the families Cecidomyiidae and Coccinellidae. Two primary parasitoids, Leptomastix dactylopii Howard (Encyrtidae) and Coccidoxenoides perminutus (Girault) (=Pauridia peregrina Timberlake, =Coccidoxenoides peregrinus (Timberlake)) (Encyrtidae), were reared from different mealybug stages, along with several hyperparasitoids. The primary parasitoids were probably introduced fortuitously. These diverse natural enemies were recovered throughout the sampling period from the different habitat types. The identifica- tion of key natural enemies associated with P. minor has important implications for the implementation of biological control in newly infested areas.

The West Indian fruit fly, Anastrepha obliqua (Macquart), infests numerous fruit species, particularly Anacardiaceae and most importantly mango (Mangifera indica L.). Widespread in the Neotropics, it was first reported in Hispaniola nearly 70 years ago. Continental populations are attacked by the opiine braconid parasitoids Utetes anastrephae (Viereck) and Doryctobracon areolatus (Szepligeti). Largely sympatric, the two species co-exist through microhabitat specializations based on different ovipositor lengths and asymmetries in larval competitive abilities during multiparasitism. Utetes anastrephae, but not D. areolatus, is apparently native to the Dominican Republic. Since the two parasitoids share an evolutionary history over a substantial portion of their distributions it was proposed that 1) D. areolatus would find the Dominican environment suitable, as does U. anastrephae; and 2) that there would be no negative interactions when the two species were reunited and overall parasitism would inc...

Pheromone-food-bait trap and acoustic surveys were conducted in Curacao to monitor a recently discovered invasion of Rhynchophorus ferrugineus L. (RPW). This pest of economic importance in regions of Asia, the Middle East, and the Mediterranean was not observed in the Americas until 2009. Due to its economic and environmental damage, there is an urgent need to manage or eradicate RPW in Curacao to reduce its impact on the island as well as avoid the possibility of transference to surrounding regions. Studies were conducted to explore methods available for monitoring adults with traps and acoustically assessing larval infestations in trees in the warm, dry but humid Curacao environment - considering also some special challenges of urban conditions, such as increased traffic noise or unwanted human curiosity that could negatively affect monitoring success. Bucket traps baited with 4-methyl-5-nonanol/4-methyl-5-nonanone pheromone lure, ethyl acetate and a molasses - ethylene glycol mixture captured ...

Raoiella indica has spread rapidly through the Neotropical region where the mite damages economically and ecologically important plants. Three studies were conducted to determine the host plant range of R. indica, using the presence of colonies containing all life stages as an indicator of reproductive suitability. Periodic surveys at the Fairchild Tropical Botanic Garden (Miami Dade County, FL, USA) and the Royal Botanical Gardens (Port of Spain, Trinidad and Tobago) identified 27 new reproductive host plants. The reproductive suitability of two dicotyledonous species and three native Florida palm species was examined. An updated list of reproductive host plants of R. indica is presented. All reported reproductive hosts (91 plant species) of R. indica are monocots from the orders Arecales (Arecaceae), Zingiberales (Heliconiaceae, Musaceae, Strelitziaceae, Zingiberaceae) and Pandanales (Pandanaceae). Most are palms of the family Arecaceae that originated in areas of the Eastern Hemisphere; about one fourth of the reported hosts are native to the New World and could be considered new host associations of R. indica. Six years after the initial detection in the Caribbean, R. indica has expanded its host plant range. Here we report 27 new reproductive host of R. indica that represent 30% of increase on previous host plant records. As this mite continues spreading in the Neotropical region a great diversity of plants is potentially affected.

Rhynchophorus ferrugineus, an important palm pest, was accidentally introduced into the Caribbean. A monitoring programme was established to determine the population level and distribution of infestations on Aruba and Curacao through the use of commercially available pheromone traps. Due to the small size of the islands and limited distribution of palms, eradication may be feasible using a combination of trapping, timely disposal of infested palms and curative and prophylactic chemical treatments. These studies on the pest in the Caribbean were used to help design a USDA plant health emergency response through the development of Animal Plant Health Inspection Service, Plant Protection and Quarantine New Pest Response Guidelines and provide an effective emergency response programme for other Caribbean Islands and the Americas.

Tephritid fruit flies are serious economic pests worldwide. As larvae, they feed and develop within the pulp of host fruits, making infestation difficult to detect by visual inspection. At U.S. ports of entry, incoming produce shipments are checked for infestation by manually cutting open a small sample of fruit and searching for tephritid larvae. Consequently, there is a need for more sensitive, high-throughput screening methods. This study evaluated gas chromatography (GC) as a potential technology for improved detection of hidden infestation. Grapefruits ( Citrus × paradisi Macfad.) infested with immature stages of the Caribbean fruit fly Anastrepha suspensa (Loew) (Diptera: Tephritidae) were examined to determine if infested fruit emitted a chemical profile distinct from that of non-infested fruit. Peaks identified by GC analysis were grouped into three classes. Chemicals detected in similar quantities in all samples, or slightly elevated in infested samples, were regarded as non-diagnostic background volatiles. Chemicals highly elevated after oviposition, during the last instar exit stage, and in experimentally-pierced fruit were interpreted to be indicators of citrus peel injury, and included d -limonene and β-ocimene. Chemicals elevated exclusively in the larval infestation stages were considered indicators of feeding damage and potentially diagnostic of infestation, and included hexyl butanoate and an unidentified compound. The peaks associated with injury and feeding were also detectable with a portable ultra-fast GC analyzer that required less than 80 s per sample. Further studies will investigate the potential application of these results for development of a rapid, non-destructive screening method for detection of tephritid infestation.

Acoustic devices provide nondestructive, remote, automated detection, and monitoring of hidden insect infestations for pest managers, regulators, and researchers. In recent decades, acoustic devices of various kinds have been marketed for field use, and instrumented sample containers in sound-insulated chambers have been developed for commodity inspection. The efficacy of acoustic devices in detecting cryptic insects, estimating population density, and mapping distributions depends on many factors, including the sensor type and frequency range, the substrate structure, the interface between sensor and substrate, the assessment duration, the size and behavior of the insect, and the distance between the insects and the sensors. Considerable success has been achieved in detecting grain and wood insect pests. Microphones are useful sensors for airborne signals, but vibration sensors interface better with signals produced in solid substrates, such as soil, grain, or fibrous plant structures. Ultrasonic sensors are particularly effective for detecting wood-boring pests because background noise is negligible at > 20 kHz frequencies, and ultrasonic signals attenuate much less rapidly in wood than in air; grain, or soil. Problems in distinguishing sounds produced by target species from other sounds have hindered usage of acoustic devices, but new devices and signal processing methods have greatly increased the reliability of detection. One new method considers spectral and temporal pattern features that prominently appear in insect sounds but not in background noise, and vice versa. As reliability and ease of use increase and costs decrease, acoustic devices have considerable future promise as cryptic insect detection and monitoring tools.

This study was conducted to determine the density, the incidence of parasitism, and the generalist predator assemblage of the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), on orange jasmine plants [Murraya paniculata (L.) Jack] in the residential landscape of Miami-Dade County, Florida. Flush shoots (10 cm) were collected from ten orange jasmine hedges in each of the four communities (Doral, Coral Gables, Palmetto Bay, and Homestead). We did not detect any consistent pattern in the fluctuation of psyllid density over time in the four communities. The greatest densities of eggs (65.5 ± 36.3 eggs/shoot), early instars (first to third) (87.2 ± 47.8 nymphs/shoot), and late instars (fourth and fifth) (16.9 ± 9.3 nymphs/shoot) were detected on 24 May 2006 in Palmetto Bay (egg) and Doral (early and late instars). The density of adult psyllids remained below two individuals per flush shoot at all locations for the entire sampling period. There were no consistent correlations bet...

Research was conducted in Florida to assess the phenology of pink hibiscus mealybug, Maconellicoccus hirsutus (Green), based on numbers of adult males captured in traps baited with a synthetic pheromone. Trapping was conducted at 3 locations in east central Florida in ornamental plantings of hibiscus (Hibiscus rosa-sinensis L.) infested by the mealybug, with traps hung directly within plants. Trapping was also conducted in southeast Florida near Miami in a tropical fruit tree planting. Males were highly attracted to traps baited with the lure, consistent with reports in the literature. Numbers of adult males captured in traps indicated that M. hirsutus was consistently most abundant during late summer and early fall with peak populations occurring anytime during late Aug through early Oct. Populations of M. hirsutus, based on captures of males, were consistently low during winter and spring from Jan through mid Apr.

Important life history parameters of the mealybug, Maconellicoccus hirsutus (Green), were characterized on hibiscus (Hibiscus rosa-sinensis L.) cuttings at six constant temperatures between 15 and 35 degrees C. The development of M. hirsutus was the fastest at 27 degrees C, where the mealybugs completed development in approximately 29 d. The lower (T(min)) and upper (T(max)) developmental thresholds and the optimal developmental temperature (T(opt)) for the development of female mealybugs were estimated as 14.5, 35, and 29 degrees C, respectively. The thermal constant (K), which is the number of temperature-day or degree-day units required for development, of the females was 347 DD. The original distribution range prediction (based on T(min) = 17.5 degrees C and K = 300 DD) indicated that M. hirsutus could complete at least one generation in all of the continental United States. However, results of this study suggested that the distribution range of M. hirsutus may expand northward because of the lower T(min), and the predicted number of generations in a year may be lower because of the higher K required to complete each generation. The average cumulative survival rate of M. hirsutus at 25 and 27 degrees C was 72%, which was significantly higher than 51 and 62% at 20 and 30 degrees C, respectively. M. hirsutus reproduced sexually, with each mated female producing 260-300 eggs between 20 and 27 degrees C but only approximately 100 eggs at 30 degrees C. Female longevity was reduced from 28 d at 20 degrees C to 19-21 d at 25-30 degrees C. At 27 degrees C, the net reproductive rate (R(o)) was estimated at 165 female symbol/female symbol, the intrinsic rate of population increase (r(m)) was 0.119 (female symbol/female symbol/d), the generation time (T(G)) was 43 d, and the doubling time (DT) was 5.8 d. The life table statistics suggested that the currently released biological control agents, which have higher r(m) than M. hirsutus, will be able to complete more generations than the mealybug within the tested temperature range; thus, they are effective against M. hirsutus.

The pink hibiscus mealybug, Maconellicoccus hirsutus (Green), threatens numerous crops of economic importance and could spread from populations in California and Florida to 33 other states. Field experiments conducted in Florida evaluated 3 commercially available trap designs baited with synthetic female sex pheromone for efficiency in trapping adult male M. hirsutus as well as ease in processing. Delta traps and double-sided sticky cards captured more males than Jackson traps. The Delta and Jackson traps were more effective at minimizing the capture of non-target insects. The effect of lure age on males captured was also evaluated by pre-aging lures outdoors for 0 to 8 months before testing. Fewer males were caught in Delta traps as the age of the lure increased, with significantly fewer caught in traps that had been pre-aged for 2 months. Monitoring of male flight activity throughout diel cycle with baited Delta traps indicated that males were most active around dusk. The field experiments also showed that the pheromone traps often capture males in areas where no visual indication of an infestation is evident. The pheromone trap may serve as a valuable tool to detect new infestations of pink hibiscus mealybug.

Tuta absoluta Meyrick (Lepidoptera: Gelechiidae), a pest of tomato, was recently detected in Panama in Central America and now threatens to expand into the important tomato production areas of Mexico and the United States. Moths caught in T. absoluta pheromone-baited traps must be removed and dissected to confirm the species present before containment and mitigation strategies are put in place. Timely processing of traps can be hindered by the presence of numerous similar nontarget moths that cannot be easily prescreened. Trapping systems using dry bucket traps or Delta traps with either hot melt pressure sensitive adhesives (HMPSA) or cool melt adhesives were evaluated for their effectiveness in trapping T. absoluta and for their ease in allowing identification of nontarget moths. Delta traps in Panama with HMPSA and cool melt adhesives both trapped T. absoluta with equal efficacy. In Florida, nontarget moths were easier to prescreen from bucket traps and HMPSA inserts. Importantly, moths found in bucket traps as well as on cool melt adhesive inserts were of a lower quality than those on HMPSA inserts, making identification more difficult. Studies conducted in Florida and Panama tomato and potato fields showed that commercially produced pheromones containing only the main pheromone component ((3E, 8Z, 11Z)-tetradecatrien-1-yl acetate) or containing both the main and minor pheromone component ((3E, 8Z)-tetradecadien-1-yl) attracted nontarget moths. Survey programs, particularly large-scale ones, should consider the application of alternative trapping systems or new adhesives available in order to facilitate the visual prescreening of nontarget moths.

Our previous field studies suggested that the two chiral centers in the sex pheromone of pink hibiscus mealybug, Maconellicoccus hirsutus, could elicit different male responses. The chiral center in the acid moiety of the pheromone seemed to be more critical than the alcohol portion of the pheromone molecule for attractiveness. The objective of the current study was to test this hypothesis by deploying stereoisomeric blends in pheromone traps. Captures of male M. hirsutus showed that pheromone with the naturally occurring (R)-maconelliyl (S)-2-methylbutanoate and (R)-lavandulyl (S)-2-methylbutanoate [R-S configuration] was most attractive and that pheromone with the unnatural S-S configuration was less attractive. In addition, the RS-R blend (containing R-R and S-R stereoisomers) yielded captures of male M. hirsutus that were comparable to blank controls, and an inhibitory effect was observed when R-R and S-R were combined with naturally occurring R-S blend. These results suggest a unique chirality recognition mechanism; olfactory discrimination among different pheromone stereoisomers depends upon both asymmetric centers. The S configuration on the acid moiety elicits attraction, whereas the R configuration induces inhibition. However, the attractive activity shows some degree of tolerance toward chirality change in the alcohol portion of the pheromone molecules.

Foreign exploration to search for natural enemies of the invasive plant tropical soda apple, Solarium viarum Dunal (Solanaceae) were conducted initially in 1994 by University of Florida researchers in collaboration with Universidade Estadual Paulista, Jaboticabal campus, Brazil. Sixteen species of insects were found associated with tropical soda apple in Brazil and Paraguay (Medal et al. 1996). A biological control program was initiated by the University of Florida in 1997 with funds provided by the United States Department of Agriculture, Animal and Plant Health Inspection Service, and the Florida Department of Agriculture & Consumer Services. Explorations in the place of origin (southern Brazil, northeast Argentina, south Paraguay, and Uruguay) of the target weed were conducted in collaboration with Brazilian university researchers, and the USDAAgriculture Research Service Biological Control Laboratory in Hurlingham, Argentina. During exploratory surveys, the leaf beetle Gratiana boliviana Spaeth (Coleoptera: Chrysomelidae) was found on tropical soda apple plants in southern Brazil. A high level of specificity and significant defoliation of tropical soda apple were demonstrated in host-specificity tests conducted at the Florida Biological Control Laboratory quarantine facilities in Gainesville, the USDA-ARS (Agriculture Research Service) South American Biological Control Laboratory in Argentina, and the USDAARS quarantine facilities in Stoneville, Mississippi, as well as in extensive field surveys and open-field tests conducted in South America. After five years of intensive plant feeding and oviposition tests, the South-American leaf beetle G.boliviana was the first biological control agent approved for field release (7 May 2003) by the USDA-APHIS-PPQ in the southeastern United States to join the battle against tropical soda apple also known by the nickname the 'plant from heir (Coile 1993). This exotic weed has invaded over half a million hectares of grasslands and natural areas in at least 6 states (Florida, Alabama, Georgia, Mississippi, South Carolina, and Texas). Intensive host-specificity tests including 126 plant species in 35 families were conducted with G. boliviana from 1998 to 2001 (Medal et al. 2002, 2003, 2004). Initial releases of G boliviana in Florida began in summer 2003, and currently approximately 200,000 G. boliviana immature and adults have been released in 37 Florida counties, 14,000 in 2 counties in Georgia, 18,000 in 3 counties in Alabama, and 825 in 1 county in Texas. In th s article we report the establishment, post-release monitoring, and initial impacts of G. boliviana on tropical soda apple plants in Sumter County, Florida. A field release of G. boliviana was made in a

Accurate identification of plant pests is essential to maintaining a successful plant biosecurity programme. Diagnostic methods and technologies used by national regulatory programmes (NPPOs), and associated laboratories for identification, are driven by method/protocol availability, taxonomy and biology of the pest, all informed by accurate reference collections and genetic databases. The ultimate selection and implementation of specific diagnostics for any programme is influenced by the unique circumstances faced by each plant protection organization. While regulatory diagnostics are used to protect agriculture and the environment, they also must be accomplished while maintaining open agricultural trade. Much information is needed to fit the diagnostic to its intended use and avoid improper use or assay failure. The components that currently drive method development and deployment of systems for regulatory identification and diagnosis include criteria for method selection, development, and transfer to the field. Diagnostic methods that are developed using quality management guidelines and appropriate validation strategies can produce universally acceptable results for regulatory decisions. Technologies that are serological, genome based, or detect volatile signatures can supplement morphological and visual identification as well as independently provide accurate identification of regulatory pests. Each NPPO continues to evolve its utility to pest detection by advancing new technologies such as DNA barcodes, recombinant DNA produced monoclonal antibodies, and novel ways of detecting targeted nucleic acids. Use of two or more assays to fulfill regulatory requirements adds diversity for cross-checking and accountability of the results when high-consequence regulatory decisions are made.

Nations have designed biosecurity systems to protect their animal, plant, and environmental resources from invasion by pests. Surveillance serves as a key component of that regulatory continuum. This chapter discusses “surveillance” and touches on many topics associated with it: Sampling, detection thresholds, Geographic Information Systems (GIS), and cyberinfrastructure.

A pheromone-based system to locate and monitor Planococcus minor (Maskell), a pest of over 250 plants including citrus, grape, and cacao, was tested. The difficulty in distinguishing P. minor from the citrus mealybug, P. citri, makes finding and evaluating the impact of the pest challenging. Studies conducted in Puerto Rico determined that synthetic P. minor pheromone lures preaged 120 d in the field caught similar number of males as lures not aged (fresh). Molecular analysis of trapped mealybug males using mitochondrial cytochrome oxidase-1, the internal transcriber space two locus, and 28S-D2 gene showed the pheromone traps to be species specific. Traps baited with P. minor pheromone were used to monitor the pest in south Florida and to locate potential infestations. P. minor males were found at all locations studied in South Florida and were present in low numbers (1.03 +/- 0.69 mean +/- SE/trap/14 d). Over 14,000 terminals, fruit, and flowers were visually inspected over a 6 mo period of peak trap catches before the first adult P. minor female was found. The synthetic pheromone lures proved to be an effective tool to locate and monitor this pest new to the continental United States.

This chapter describes the host range, economic impact, pest status, origin and distribution, molecular identification, biology, life history, rearing techniques, sampling and monitoring, damage and economic thresholds, and control tactics (including chemical, biological, ant control, mating disruption, mass trapping, lure and kill, cultural, physical, mechanical, quarantine, and host plant resistance) of Planococcus minor.

This chapter presents a study conducted to examine Citrus fruits infested with A. suspensa to determine if the infested fruits emitted a detectable chemical profile distinct from that of non-infested Citrus fruits. Samples of headspace volatiles were collected at various stages of infestation and chemical analysis was performed with several types of GC equipment. Since the primary goal was development of a rapid screening protocol for 'signature chemicals', the majority of analyses were performed with a rapid (9 min) GC separation method. To evaluate the efficacy of separation with this rapid method, and to identify the volatile chemical components, a slower (25 min) high-resolution GC separation was performed in combination with mass spectral analysis. A preliminary evaluation was carried out of a portable ultra-high-speed GC analyser for detection of these same chemicals using a method requiring less than 80 s for sampling and chemical analysis. The results of the study indicate that there are GC-detectable volatile chemicals associated with tephritid infestation of fruit commodities. With infested grapefruits, the chemicals can be distinguished as those indicative of Citrus peel injury and those correlated with larval feeding (pulp/albedo injury). Of the chemicals identified, none appeared to be insect-produced, but rather natural fruit volatiles occurring at higher levels than normal. Elevated levels of δ-limonene and β-ocimene are only indicative of puncture wounds or other external damage to the grapefruit peel. However, if these two chemicals are accompanied by elevated levels of hexyl butanoate and the (as of yet) unidentified compound, this volatile profile is potentially diagnostic of Citrus infestation.

The red palm mite (Raoiella indica), an invasive pest of coconut, entered the Western hemisphere in 2004, then rapidly spread through the Caribbean and into Florida, USA. Developing effective sampling methods may aid in the timely detection of the pest in a new area. Studies were conducted to provide and compare intra tree spatial distribution of red palm mite populations on coconut in two different geographical areas, Trinidad and Puerto Rico, recently invaded by the mite. The middle stratum of a palm hosted significantly more mites than fronds from the upper or lower canopy and fronds from the lower stratum, on average, had significantly fewer mites than the two other strata. The mite populations did not vary within a frond. Mite densities on the top section of the pinna had significantly lower mite densities than the two other sections, which were not significantly different from each other. In order to improve future sampling plans for the red palm mite, the data was used to estimate the variance components associated with the various levels of the hierarchical sampling design. Additionally, presence-absence data were used to investigate the probability of no mites being present in a pinna section randomly chosen from a frond inhabited by mites at a certain density. Our results show that the most precise density estimate at the plantation level is to sample one pinna section per tree from as many trees as possible.

The ability of the invasive lobate lac scale, Paratachardina pseudolobata Kondo & Gullan, to survive exposure to near or below freezing temperatures (-25, -15, -10, -5, 0, 5, and 10°C) for extended durations (6, 12, 24, 48, and 72 h) was assessed in the laboratory. Exposure at 10°C for 6 to 72 h did not cause significant mortality in immature and adult lobate lac scales. All nymphs and adults were killed after exposure to very low temperatures (-25 to -10°C) for 6 h. All nymphs were killed within 24 h at -5, 0, and 5 oC, while about 2% of adults were still alive after 72 h at 0 and 5°C. Results suggested that the range expansion of the lobate lac scale in the United States will be limited by winter temperature. The lobate lac scale may have a higher potential to invade areas where the average winter temperature do not drop below freezing for an extended duration (3 or more consecutive days), such as southern Florida, Rio Grande Valley in Texas, and Colorado river Delta between California and Arizona.

s, Orlando, FL, USA, p. 30. Mullahey, J.J. and Colvin, D.L. (1993) Tropical soda apple: a new noxious weed in Florida. University of Florida, Florida Cooperative Extension Service, Fact Sheet WRS

Lepidopteran larvae oral secretions and regurgitant (R), which contain a plethora of potential elicitors, are known to dramatically change a plant's wound response. We demonstrate, with a detailed microarray and secondary metabolite analysis, that the two most abundant fatty acid-amino acid conjugates (FACs) in the R of the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) can account for all measured direct (trypsin proteinase inhibitor: TPI) and indirect (cis-alpha-bergamotene) defences, the endogenous jasmonic acid burst that elicits them, and 86% of the induced transcriptional changes (89% up and 83% down) in its native host Nicotiana attenuata and hence are necessary and sufficient for the Manduca-specific modulation of the wound response. FACs were not found in eggs, but detected in larvae of all instars after their first meal. FACs were found in all regions of the alimentary canal and in the frass, but did not occur in salivary or mandibular glands, extracts of which were not active in any assay. Individual larvae differed substantially in their FAC composition and two FAC chemotypes were discernible: N-linolenoyl-L-glutamine biased R and N-linolenoyl-L-glutamate biased R. We created synthetic blends of FACs to mimic these chemical phenotypes and determined whether plants respond differently to the different R chemotypes. Micorarray and TPI analysis revealed that plants do not differentiate. N. attenuata plants use FACs from feeding caterpillars to tailor their wound responses but do not use the variability in FAC ratios to recognize attack from an individual caterpillar.