Search

Microarray transcript analysis of human renal transplantation biopsies has successfully identified the many patterns of graft rejection. To evaluate an alternative, this report tests whether gene expression from the Banff Human Organ Transplant (B-HOT) probe set panel, derived from validated microarrays, can identify the relevant allograft diagnoses directly from archival human renal transplant formalin-fixed paraffin-embedded biopsies. To test this hypothesis, principal components (PCs) of gene expressions were used to identify allograft diagnoses, to classify diagnoses, and to determine whether the PC data were rich enough to identify diagnostic subtypes by clustering, which are all needed if the B-HOT panel can substitute for microarrays.RNA was isolated from routine, archival formalin-fixed paraffin-embedded tissue renal biopsy cores with both rejection and nonrejection diagnoses. The B-HOT panel expression of 770 genes was analyzed by PCs, which were then tested to determine their ability to identify diagnoses.PCs of microarray gene sets identified the Banff categories of renal allograft diagnoses, modeled well the aggregate diagnoses, showing a similar correspondence with the pathologic diagnoses as microarrays. Clustering of the PCs identified diagnostic subtypes including non-chronic antibody-mediated rejection with high endothelial expression. PCs of cell types and pathways identified new mechanistic patterns including differential expression of B and plasma cells.Using PCs of gene expression from the B-Hot panel confirms the utility of the B-HOT panel to identify allograft diagnoses and is similar to microarrays. The B-HOT panel will accelerate and expand transcript analysis and will be useful for longitudinal and outcome studies.

To seek insights into the pathogenesis of chronic active antibody-mediated rejection (CAMR), we performed mRNA analysis and correlated transcripts with pathologic component scores and graft outcomes.We utilized the NanoString nCounter platform and the Banff Human Organ Transplant gene panel to quantify transcripts on 326 archived renal allograft biopsy samples. This system allowed correlation of transcripts with Banff pathology scores from the same tissue block and correlation with long-term outcomes.The only pathology score that correlated with AMR pathways in CAMR was peritubular capillaritis (ptc). C4d, cg, g, v, i, t, or ci scores did not correlate. DSA-negative CAMR had lower AMR pathway scores than DSA-positive CAMR. Transcript analysis in non-CAMR biopsies yielded evidence of increased risk of later CAMR. Among 108 patients without histologic CAMR, 23 developed overt biopsy-documented CAMR within 5 years and as a group had higher AMR pathway scores (Peritubular capillary inflammation and DSA are the primary drivers of AMR transcript elevation. Transcripts revealed subpathological evidence of AMR, which often preceded histologic CAMR and subpathological evidence of TCMR that predicted graft loss in CAMR.

Cet article se concentre sur la troisième Biennale de La Havane (1989) et analyse la conception de la contemporanéité internationale que propose Reiko Tomii afin de diversifier l’histoire des biennales. La contemporanéité internationale mobilise des pratiques locales pour construire des analyses globales qui développent une compréhension polycentrique de l’histoire mondiale de l’art. De même, l’approche commissariale de cette troisième édition visait à décentrer l’art contemporain, en créant une exposition pour les milieux artistiques marginalisés à un moment de tensions politiques, culturelles et économiques à Cuba, et de crise politique mondiale. La troisième Biennale de La Havane s’opposait aux discours eurocentriques dominants de l’histoire de l’art en préconisant la coexistence des multiples arts contemporains.

In this study, 302 patients who were completing Phase II cardiac rehabilitation were assessed at their entrance to cardiac rehabilitation and at 3 months. After 3 months, subjects improved on 8 of 9 dimensions. Greatest gains were in Fitness (22.5%, P < .0001) and Daily Activities (24.4%, P < .0001). Despite stratification by gender, a sedentary lifestyle, and tobacco use, quality of life was markedly improved at the completion of rehabilitation. Completion of a Phase II cardiac rehabilitation program can lead to statistically significant increases in the quality of life following consequential cardiac events.