12 results on '"Amin Yu"'
Search Results
2. Neuronal Aquaporin 1 Inhibits Amyloidogenesis by Suppressing the Interaction Between Beta-Secretase and Amyloid Precursor Protein
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Sic L. Chan, Amin Yu, Jogi V. Pattisapu, Yoonsuk Cho, Dong-Gyu Jo, Srinivasulu Chigurupati, Seung Hyun Baek, Mark P. Mattson, Jinsu Park, Mohamed R Mughal, and Meenu Madan
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Aging ,THE JOURNAL OF GERONTOLOGY: Biological Sciences ,Amyloid ,Immunoprecipitation ,Amyloid beta-Protein Precursor ,Mice ,Downregulation and upregulation ,Alzheimer Disease ,mental disorders ,Amyloid precursor protein ,Medicine ,Animals ,Humans ,Neurons ,Gene knockdown ,biology ,Aquaporin 1 ,business.industry ,Cell biology ,Disease Models, Animal ,medicine.anatomical_structure ,Cerebral cortex ,biology.protein ,Ectopic expression ,Geriatrics and Gerontology ,Amyloid Precursor Protein Secretases ,business ,Amyloid precursor protein secretase - Abstract
The accumulation of amyloid-β (Aβ) is a characteristic event in the pathogenesis of Alzheimer’s disease (AD). Aquaporin 1 (AQP1) is a membrane water channel protein belonging to the AQP family. AQP1 levels are elevated in the cerebral cortex during the early stages of AD, but the role of AQP1 in AD pathogenesis is unclear. We first determined the expression and distribution of AQP1 in brain tissue samples of AD patients and two AD mouse models (3xTg-AD and 5xFAD). AQP1 accumulation was observed in vulnerable neurons in the cerebral cortex of AD patients, and in neurons affected by the Aβ or tau pathology in the 3xTg-AD and 5xFAD mice. AQP1 levels increased in neurons as aging progressed in the AD mouse models. Stress stimuli increased AQP1 in primary cortical neurons. In response to cellular stress, AQP1 appeared to translocate to endocytic compartments of β- and γ-secretase activities. Ectopic expression of AQP1 in human neuroblastoma cells overexpressing amyloid precussir protein (APP) with the Swedish mutations reduced β-secretase (BACE1)-mediated cleavage of APP and reduced Aβ production without altering the nonamyloidogenic pathway. Conversely, knockdown of AQP1 enhanced BACE1 activity and Aβ production. Immunoprecipitation experiments showed that AQP1 decreased the association of BACE1 with APP. Analysis of a human database showed that the amount of Aβ decreases as the expression of AQP1 increases. These results suggest that the upregulation of AQP1 is an adaptive response of neurons to stress that reduces Aβ production by inhibiting the binding between BACE1 and APP.
- Published
- 2020
3. Influence of chitosan addition on the properties and characteristics of acrylic acid-chitosan based superabsorbent prepared through gamma irradiation technique and its application for urea loading
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Jayanudin Jayanudin, Retno Sulistyo D. Lestari, Dhena Ria Barleany, Alia Badra Pitaloka, Meri Yulvianti, Harly Demustila, Amin Yulianto, Dendy Vito Anggoro, and Adam Ruhiatna
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gamma irradiation ,superabsorbent ,swelling ,urea loading ,water retention ,Technology - Abstract
Superabsorbent can be used as a carrier for urea-fertilizer which can release urea slowly and can be used as a dry soil moisturizer. The main goal of the current study was to investigate the influence of adding chitosan grafted onto acrylic acid as a superabsorbent base material on the properties and characteristics of the resulting superabsorbents. The method used to prepare the superabsorbent was the gamma irradiation technique. Acrylic acid was neutralized by adding KOH, and then chitosan was added. The mixture was put in a plastic container, followed by irradiation with gamma rays. The formed superabsorbent was washed and dried in an oven, then the dry superabsorbent was analyzed for its properties and characteristics. The results showed that superabsorbent properties such as swelling ratio (from 49.38 g/g to 49.38 g/g), water retention (0.43%-0.89% after 7 days), and urea loading (86.75% to 96.4%) were affected by the amount of chitosan added. However, adding too much chitosan can reduce the superabsorbent properties. The characterization of the superabsorbent revealed a smooth surface with a porous structure, and FTIR analysis showed that chitosan was grafted onto acrylic acid.
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- 2023
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- View/download PDF
4. Effect of acetazolamide on aquaporin-1 and fluid flow in cultured choroid plexus
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Pouya A, Ameli, Meenu, Madan, Srinivasulu, Chigurupati, Amin, Yu, Sic L, Chan, and Jogi V, Pattisapu
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Time Factors ,Aquaporin 1 ,Rhodamines ,Dextrans ,Rats ,Acetazolamide ,Capillary Permeability ,Organ Culture Techniques ,Animals, Newborn ,Gene Expression Regulation ,Choroid Plexus ,Hydrodynamics ,Animals ,RNA, Messenger ,Carbonic Anhydrase Inhibitors - Abstract
Acetazolamide (AZA), used in treatment of early or infantile hydrocephalus, is effective in some cases, while its effect on the choroid plexus (CP) remains ill-defined. The drug reversibly inhibits aquaporin-4 (AQP4), the most ubiquitous "water pore" in the brain, and perhaps modulation of AQP1 (located apically on CP cells) by AZA may reduce cerebrospinal fluid (CSF) production. We sought to elucidate the effect of AZA on AQP1 and fluid flow in CP cell cultures.CP tissue culture from 10-day Sprague-Dawley rats and a TRCSF-B cell line were grown on Transwell permeable supports and treated with 100 μM AZA. Fluid assays to assess direction and extent of fluid flow, and AQP1 expression patterns by immunoblot, Immuncytochemistry (ICC), and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) were performed.Immunoblots and ICC analyses showed a decrease in AQP1 protein shortly after AZA treatment (lowest at 12 h), with transient AQP1 reduction mediated by mRNA expression (lowest at 6 h). Transwell fluid assays indicated a fluid shift at 2 h, before significant changes in AQP1 mRNA or protein levels.Timing of AZA effect on AQP1 suggests the drug alters protein transcription, while affecting fluid flow by a concomitant method. It is plausible that other mechanisms account for these phenomena, as the processes may occur independently.
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- 2011
5. Effect of Acetazolamide on Aquaporin-1 and Fluid Flow in Cultured Choroid Plexus
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Amin Yu, Srinivasulu Chigurupati, Jogi V. Pattisapu, Meenu Madan, Pouya Ameli, and Sic L. Chan
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Organ Culture Technique ,business.industry ,Pharmacology ,Infantile hydrocephalus ,medicine.disease ,Hydrocephalus ,Cerebrospinal fluid ,Cell culture ,Aquaporin 1 ,Medicine ,Choroid plexus ,business ,Acetazolamide ,medicine.drug - Abstract
Acetazolamide (AZA), used in treatment of early or infantile hydrocephalus, is effective in some cases, while its effect on the choroid plexus (CP) remains ill-defined. The drug reversibly inhibits aquaporin-4 (AQP4), the most ubiquitous “water pore” in the brain, and perhaps modulation of AQP1 (located apically on CP cells) by AZA may reduce cerebrospinal fluid (CSF) production. We sought to elucidate the effect of AZA on AQP1 and fluid flow in CP cell cultures.
- Published
- 2011
6. Acetazolamide affects Aquaporin 1 expression of cultured choroid plexus cells
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Jogi V. Pattisapu, Srinu Chigurupathi, Meenu Madan, Sic L. Chan, Amin Yu, and Pouya Ameli
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medicine.medical_specialty ,Hematology ,business.industry ,Apical membrane ,Pharmacology ,medicine.disease ,lcsh:RC346-429 ,Hydrocephalus ,Cellular and Molecular Neuroscience ,Basal (phylogenetics) ,Aquaporin 4 ,Developmental Neuroscience ,Neurology ,Internal medicine ,Ophthalmology ,Aquaporin 1 ,medicine ,Oral Presentation ,Choroid plexus ,Acetazolamide ,business ,lcsh:Neurology. Diseases of the nervous system ,medicine.drug - Abstract
Background Acetazolamide (AZA), the only drug generally approved for hydrocephalus, is effective in only 25-30% of patients, and its effect on basal fluid flow in choroid plexus (CP) is unknown. The drug reversibly inhibits Aquaporin 4 (AQP4), the most highly expressed ‘water pore’ in the brain, and it is postulated that it reduces CSF production by modulating the expression and/or function of AQP1 (mostly found in the apical membrane of CP). In this study, we sought to elucidate the direct effect of AZA on basal fluid flow in CP.
- Published
- 2010
7. The Influence of Literacy and Read Aloud Activities on the Early Childhood Education Students’ Receptive Language Skills
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Lely Diah Eko Priyantini and Amin Yusuf
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literacy activities ,read aloud activities ,receptive language skills ,Education (General) ,L7-991 - Abstract
Stimulation on language development aspects is greatly needed since humans’ life may not be separated from language use and acquisition. This research aims at examining the influence of literacy and read aloud activities on the early childhood education students’ receptive language skills. This research is a correlational quantitative study conducted with a survey approach. The research population was the students of Early Childhood Education Institutions in South Purwokerto District, Banyumas Regency. There were 54 Early Childhood Education Institutions in South Purwokerto District divided into 7 groups/clusters. The sampling of this research was conducted by using the Slovin’s formula with the total samples of 115 students of Early Childhood Education Institutions. The data was collected through observation and then descriptively analyzed in percentages using multiple regression analysis supported with SPSS 23.0. The research results showed that literacy activities influenced the Early Childhood Education students’ receptive language skills with the t-count value of 6.620 at the significance of 0.000. Read aloud activities also influenced the Early Childhood Education students’ receptive language skills with the t-count value of 8.066 at the significance of 0.000. Literacy and read aloud activities significantly influenced the Early Childhood Education students’ receptive language skills with the f-count value of 70.386 at the significance of 0.000 and the total influence of 55.5%. Thus, it is suggested that literacy and read aloud activities should be continuously made to improve the Early Childhood Education students’ receptive language skills.
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- 2020
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8. Finite-Time Disturbance-Observer-Based Integral Terminal Sliding Mode Controller for Three-Phase Synchronous Rectifier
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Bahman Eskandari, Amin Yusefpour, Moosa Ayati, Jorma Kyyra, and Edris Pouresmaeil
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AC-DC power converters ,disturbance observer ,integral terminal sliding mode controller ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
This article is concerned with the design of a finite-time disturbance-observer-based integral terminal sliding mode controller for the effective performance of three-phase synchronous rectifiers. The proposed control technique is developed based on the conventional synchronous reference frame model of the three-phase grid-connected converter, and the system dynamics is described in terms of a time-varying non-linear state equation. The variation of DC-load is considered as a disturbance. Therefore, a combination of a fast disturbance observer and an integral terminal sliding mode controller is utilized to produce the reference value of the direct axis for the current control loop. In this research, by employing Lyapunov stability theorem in the theoretical analysis and by numerical simulations, it is confirmed that the proposed closed-loop system is stable and the states converge to desired values in finite time even in the presence of load disturbance and control input saturation. The integral terminal sliding mode controller is utilized to maintain a robust performance along with a faster response of the converter. In order to demonstrate the performance ability of the proposed control scheme under real condition, an AC power source, impregnated with low order harmonics, is assumed. A real-time laboratory setup of the synchronous rectifier has been developed successfully, and the effective performance of the proposed control technique is fully proven.
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- 2020
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9. ADL report
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Ruhollah Hamidi Motlagh, Muhammad Esmailzadeh, Saeed Soleymani, Amin Yusefi, and Mohsen Ashari
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Technology (General) ,T1-995 ,Science (General) ,Q1-390 - Published
- 2019
10. The protective effect of vitamin E and selenium on liver tissue in fructose- induced diabetic rat
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Farangis Ghassemi and amin Yuosefinasab
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antioxidant ,vitamin e ,selenium ,liver enzyme ,diabet ,Medicine - Abstract
Background: Diabetes disorder in organ's function via oxidative stress. In this research, the protective effect of vitamin E and selenium "as antioxidant" on liver function were investigeted in diabetic rats. Materials and methods: 35 adult male rats (Wistar) weighing 180 ± 20g divided randomly into control, sham and 3 experimental groups (E, S and E+S). All of groups except control were fed 140 cc fructose 10% as a daily for 9 weeks. Control group without treatment, sham were injected 0.20 cc of physiologic serum and and group (S) were injected 0.20 cc of selenium solution (0.5 mg/kg/b.wt) intraperitoneally. group (E) were gavaged 0.20 cc of vitamin E solution (0.5 mg/kg/b.wt) and Group (E + S) gavaged vitamin E and were injected selenium (0.5mg/kg/b.wt) for 9 weeks. Blood sampling of all groups and were separated for biochemical analyzes. Then liver was disected and fixed in 3% formalin. hbafty Automatic Tissue processor with 5 micrometer and were examined by light microscope. The sections with 5 micron thickness were prepared by microtome after passage in Tissue processor automatically. They were studied on light microscope. Using statistical software, SPSS 17, data were analyzed by ANOVA and groups were compared by Touki test at a significance level (P
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- 2014
11. ANALISIS KEBUTUHAN PENDIDIKAN MASYARAKAT
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Amin Yusuf
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Analysis of educational needs. ,Education (General) ,L7-991 - Abstract
Based on a belief that in order to assist in community development with appropriate education, the need for a research study that examined a variety of community needs for education and solutions. On the belief, the research with the above title aims to: (1) analyze the various needs education is urgent, and immediate solution can be resolved by the people in the village Kandri and (2) implementing affirmative action, mentoring programs and management of village libraries learning model for functional literacy tutors as a solution. Participatory methods or PRA, with the tools bervariatif data collectors, such as in-depth interviews, brainstorming, patisipatif observation, and documentation is a method used in the study. The results showed that, the library management assistance programs and rural development model functional literacy learning is an effective solution of many aspects that have been identified. The program can improve quality and expand access to coverage of services of non-formal education, and increase community participation in education development.
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- 2014
12. Acetazolamide affects Aquaporin 1 expression of cultured choroid plexus cells.
- Author
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Pattisapu, Jogi V., Ameli, Pouya, Madan, Meenu, Amin Yu, Chigurupathi, Srinu, and Chan, Sic L.
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AQUAPORINS - Abstract
An abstract of the paper "Acetazolamide Affects Aquaporin 1 Expression of Cultured Choroid Plexus Cells," by Jogi V. Pattisapu and colleagues is presented.
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- 2010
- Full Text
- View/download PDF
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