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Aims: The bacterium Vibrio parahaemolyticus causes gastroenteritis in humans after the consumption of raw seafood. Therefore, a rapid detection system that can identify the presence of V. parahaemolyticus accurately is necessary. One potential detection system under development is based on antibodies. This study aimed to map the outer membrane protein profile of V. parahaemolyticus. Methods and Results: These outer membrane proteins (OMP) may be antigenic and can be used to produce antibodies. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method was used to map the outer membrane protein profile of V. parahaemolyticus. The results of the analysis using SDS-PAGE to assess the OMP of V. parahaemolyticus show that the molecular weight (MW) of the major polypeptide band was in the range of 10-100 kDa. Overall, there were 31 bands observed, of which 11 were major bands with MW of 72, 67, 58, 44, 42, 40, 38, 37, and 10 kDa. Conclusion, Significance, and Impact of Study: The identified proteins have antigenic properties and can be used to produce antibodies for a rapid detection system (rapid test) to detect the presence of V. parahaemolyticus. [ABSTRACT FROM AUTHOR]

This study was conducted in order to obtain information on genetic variation in populations rated as superior cotton (Gossypium hirsutum L.) varieties in Balittas Malang, Indonesia. The samples used 10 varieties of cotton Kanesia series and 2 other superior varieties that are LRA 5166 and ISA 205A. Indicators of genetic diversity are the number of alleles per locus, allele frequencies, and heterozygosity values. DNA was isolated from the leaves of 3-week-old seedlings using the CTAB method. Amplification was performed using 5 SSRs primer pairs of the JESPR series. The results showed five microsatellite loci, yielding 12 alleles with a size range of 80-500 bp, with an average number of alleles per locus of 4.60. The average values of heterozygosity of the five loci was high, at 0.71. Based on the number of alleles, allele frequencies and heterozygosity values, the genetic variation sampled in the superior cotton varieties studied here is quite high. [ABSTRACT FROM AUTHOR]