1,672 results on '"Amedei A"'
Search Results
2. First Exploration of the Altered Microbial Gut–Lung Axis in the Pathogenesis of Human Refractory Chronic Cough
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Baldi, Simone, Fabbrizzi, Alessio, Di Gloria, Leandro, Pallecchi, Marco, Nannini, Giulia, D’Ambrosio, Mario, Luceri, Cristina, Bartolucci, Gianluca, Ramazzotti, Matteo, Fontana, Giovanni, Mannini, Claudia, Lavorini, Federico, and Amedei, Amedeo
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- 2024
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3. Experimental colitis in young Tg2576 mice accelerates the onset of an Alzheimer’s-like clinical phenotype
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Lorenzini, Luca, Zanella, Lorenzo, Sannia, Michele, Baldassarro, Vito Antonio, Moretti, Marzia, Cescatti, Maura, Quadalti, Corinne, Baldi, Simone, Bartolucci, Gianluca, Di Gloria, Leandro, Ramazzotti, Matteo, Clavenzani, Paolo, Costanzini, Anna, De Giorgio, Roberto, Amedei, Amedeo, Calzà, Laura, and Giardino, Luciana
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- 2024
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4. Effects of a chronotype-adapted diet on weight loss, cardiometabolic health, and gut microbiota: study protocol for a randomized controlled trial
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Dinu, Monica, Lotti, Sofia, Pagliai, Giuditta, Napoletano, Antonia, Asensi, Marta Tristan, Giangrandi, Ilaria, Marcucci, Rossella, Amedei, Amedeo, Colombini, Barbara, and Sofi, Francesco
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- 2024
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5. Experimental colitis in young Tg2576 mice accelerates the onset of an Alzheimer’s-like clinical phenotype
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Luca Lorenzini, Lorenzo Zanella, Michele Sannia, Vito Antonio Baldassarro, Marzia Moretti, Maura Cescatti, Corinne Quadalti, Simone Baldi, Gianluca Bartolucci, Leandro Di Gloria, Matteo Ramazzotti, Paolo Clavenzani, Anna Costanzini, Roberto De Giorgio, Amedeo Amedei, Laura Calzà, and Luciana Giardino
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Astrocytes ,Cytokines/chemokines ,Gut microbiota/microbiome ,Neuroinflammation ,Preclinical Alzheimer’s disease ,Systemic inflammation ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Systemic inflammation and neuroinflammation affect the natural course of the sporadic form of Alzheimer’s disease (AD), as supported by epidemiological and preclinical data, and several epidemiological studies indicate a higher prevalence of AD in patients with inflammatory bowel disease. In this study, we explored whether colitis induced by dextran sulfate sodium (DSS) in young, presymptomatic/preplaque mice worsens and/or anticipates age-dependent cognitive impairment in Tg2576, a widely used mouse model of AD. We demonstrated that DSS colitis induced in young Tg2576 mice anticipates the onset age of learning and memory deficit in the Morris water maze test. To explore potential mechanisms behind the acceleration of cognitive decline in Tg2576 mice by DSS colitis, we focused on gut microbiota, systemic inflammation and neuroinflammation markers. We observed a Firmicutes/Bacteroidetes ratio change in Tg2576 DSS animals comparable to that of elderly Tg2576 mice, suggesting accelerated microbiota aging in Tg2576 DSS mice, a change not observed in C57BL6 DSS mice. We also observed substantial differences between Tg2576 and WT mice in several inflammation and neuroinflammation-related parameters as early as 3 months of age, well before plaque deposition, a picture which evolved rapidly (between 3 and 5.5 months of age) in contrast to Tg2576 and WT littermates not treated with DSS. In detail, following induction of DSS colitis, WT and Tg2576 mice exhibited contrasting features in the expression level of inflammation-evoked astrocyte-associated genes in the hippocampus. No changes in microglial features occurred in the hippocampus between the experimental groups, whereas a reduced glial fibrillary acidic protein immunoreactivity was observed in Tg2576 vs. WT mice. This finding may reflect an atrophic, “loss-of-function” profile, further exacerbated by DSS where a decreased of GFAP mRNA expression level was detected. In conclusion, we suggest that as-yet unidentified peripheral mediators evoked by DSS colitis and involving the gut-brain axis emphasize an astrocyte “loss-of-function” profile present in young Tg2576 mice, leading to impaired synaptic morphological and functional integrity as a very early sign of AD.
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- 2024
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6. Fecal Microbiota Transplantation in Amyotrophic Lateral Sclerosis: Clinical Protocol and Evaluation of Microbiota Immunity Axis
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Niccolai, Elena, primary, Martinelli, Ilaria, additional, Quaranta, Gianluca, additional, Nannini, Giulia, additional, Zucchi, Elisabetta, additional, De Maio, Flavio, additional, Gianferrari, Giulia, additional, Bibbò, Stefano, additional, Cammarota, Giovanni, additional, Mandrioli, Jessica, additional, Masucci, Luca, additional, and Amedei, Amedeo, additional
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- 2024
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7. Editorial: Dietary patterns affecting cardiovascular health
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Galya Bigman and Amedeo Amedei
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cardiovascular health ,cardiovascular diseases (CVDs) ,cardiometabolic outcomes ,dietary patterns ,sodium ,fruits ,Nutrition. Foods and food supply ,TX341-641 - Published
- 2024
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8. mini-Complexome Profiling (mCP), an FDR-controlled workflow for global targeted detection of protein complexes
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Hugo Amedei, Niels Benjamin Paul, Brian Foo, Lisa Neuenroth, Stephan E. Lehnart, Henning Urlaub, and Christof Lenz
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proteome ,protein complex ,protein interaction ,complexome ,targeted proteomics ,blue native ,Analytical chemistry ,QD71-142 - Abstract
IntroductionCo-fractionation mass spectrometry couples native-like separations of protein-protein complexes with mass spectrometric proteome analysis for global characterization of protein networks. The technique allows for both de novo detection of complexes and for the detection of subtle changes in their protein composition. The typical requirement for fine-grained fractionation of >80 fractions, however, translates into significant demands on sample quantity and mass spectrometric instrument time, and represents a significant barrier to experimental replication and the use of scarce sample material (ex. patient biopsies).MethodsWe developed mini-Complexome Profiling (mCP), a streamlined workflow with reduced requirements for fractionation and, thus, biological material and laboratory and instrument time. Soluble and membrane-associated protein complexes are extracted from biological material under mild conditions, and fractionated by Blue Native electrophoresis using commercial equipment. Each fraction is analysed by data-independent acquisition mass spectrometry, and known protein complexes are detected based on the coelution of known components using a novel R package with a controlled false discovery rate approach. The tool is available to the community on a GitHub repository.ResultsmCP was benchmarked using HEK293 cell lysate and exhibited performance similar to established workflows, but from a significantly reduced number of fractions. We then challenged mCP by performing comparative complexome analysis of cardiomyocytes isolated from different chambers from a single mouse heart, where we identified subtle chamber-specific changes in mitochondrial OxPhos complexes.DiscussionThe reduced sample and instrument time requirements open up new applications of co-fractionation mass spectrometry, specifically for the analysis of sparse samples such as human patient biopsies. The ability to identify subtle changes between similar tissue types (left/right ventricular and atrial cardiomyocytes) serves as a proof of principle for comparative analysis of mild/asymptomatic disease states.
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- 2024
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9. Immune landscape and oncobiota in HPV-Associated Colorectal Cancer: an explorative study
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Ambrosio, Maria Raffaella, Niccolai, Elena, Petrelli, Federica, Di Gloria, Leandro, Bertacca, Gloria, Giusti, Andrea, Baldi, Simone, Cavazzana, Andrea, Palmeri, Matteo, Perotti, Bruno, Ramazzotti, Matteo, Arganini, Marco, and Amedei, Amedeo
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- 2023
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10. Comparison of microbial communities and the profile of sulfate-reducing bacteria in patients with ulcerative colitis and their association with bowel diseases: a pilot study
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Ivan Kushkevych, Kristýna Martínková, Lenka Mráková, Francesco Giudici, Simone Baldi, David Novak, Márió Gajdács, Monika Vítězová, Dani Dordevic, Amedeo Amedei, and Simon K.-M. R. Rittmann
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gut microbiota ,ulcerative colitis ,gut dysbiosis ,sulfate-reducing bacteria ,inflammatory bowel disease ,16s rrna gene sequencing ,Biology (General) ,QH301-705.5 - Abstract
Considerable evidence has accumulated regarding the molecular relationship between gut microbiota (GM) composition and the onset (clinical presentation and prognosis of ulcerative colitis (UC)). In addition, it is well documented that short-chain fatty acid (SCFA)-producing bacteria may play a fundamental role in maintaining an anti-inflammatory intestinal homeostasis, but sulfate- and sulfite reducing bacteria may be responsible for the production of toxic metabolites, such as hydrogen sulfide and acetate. Hence, the present study aimed to assess the GM composition – focusing on sulfate-reducing bacteria (SRB) – in patients with severe, severe-active and moderate UC. Each one of the six enrolled patients provided two stool samples in the following way: one sample was cultivated in a modified SRB-medium before 16S rRNA sequencing and the other was not cultivated. Comparative phylogenetic analysis was conducted on each sample. Percentage of detected gut microbial genera showed considerable variation based on the patients’ disease severity and cultivation in the SRB medium. In detail, samples without cultivation from patients with moderate UC showed a high abundance of the genera Bacteroides, Bifidobacterium and Ruminococcus, but after SRB cultivation, the dominant genera were Bacteroides, Klebsiella and Bilophila. On the other hand, before SRB cultivation, the main represented genera in patients with severe UC were Escherichia-Shigella, Proteus, Methanothermobacter and Methanobacterium. However, after incubation in the SRB medium Bacteroides, Proteus, Alistipes and Lachnoclostridium were predominant. Information regarding GM compositional changes in UC patients may aid the development of novel therapeutic strategies (e.g., probiotic preparations containing specific bacterial strains) to counteract the mechanisms of virulence of harmful bacteria and the subsequent inflammatory response that is closely related to the pathogenesis of inflammatory bowel diseases.
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- 2024
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11. Effects of a chronotype-adapted diet on weight loss, cardiometabolic health, and gut microbiota: study protocol for a randomized controlled trial
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Monica Dinu, Sofia Lotti, Giuditta Pagliai, Antonia Napoletano, Marta Tristan Asensi, Ilaria Giangrandi, Rossella Marcucci, Amedeo Amedei, Barbara Colombini, and Francesco Sofi
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Chronotype ,Diet ,Weight loss ,Cardiometabolic health ,Gut microbiota ,Randomized controlled trial ,Medicine (General) ,R5-920 - Abstract
Abstract Background Obesity and its associated health complications have become a global public health concern, necessitating innovative approaches to weight management. One emerging area of research focuses on the influence of chronotype, an individual’s preferred timing for daily activities, on eating habits, weight regulation, and metabolic health. Recent observational studies suggest that the misalignment between an individual’s chronotype and external cues, such as meal timing, may contribute to metabolic dysregulation and obesity, but evidence from intervention studies is still limited. This study protocol describes a randomized controlled trial designed to explore the effects of a chronotype-adapted diet, compared with a diet with a conventional calorie distribution, on weight loss, cardiometabolic health, and gut microbiota composition. Methods A total of 150 overweight/obese adults will be recruited for this 4-month parallel-group, randomized, two-arm, open-label, superiority trial with 1:1 allocation ratio. Participants will be randomly assigned to either the intervention group or the control group. The intervention group will receive a low-calorie chronotype-adapted diet with a calorie distribution adapted to the individual chronotype (morning or evening), optimizing meal timing according to their peak metabolic periods. The control group will follow a standardized low-calorie healthy eating plan without considering chronotype. Both diets will have equivalent daily calorie content, adjusted according to gender and starting weight. Anthropometric measurements, body composition, blood, and fecal samples will be obtained from each participant at the beginning and the end of the study. The primary outcome is weight change from baseline. Secondary outcomes are changes from baseline in body mass index (BMI), fat mass, lipid and glycemic profile, fecal microbiota profile, and short-chain fatty acids (SCFAs). Discussion The results of this randomized controlled trial have the potential to advance our understanding of the complex interactions between chronotype, diet, body weight, and health outcomes. By providing evidence for personalized dietary interventions based on individuals’ circadian preferences, this research could offer insights into personalized nutrition strategies. Such knowledge could guide the development of innovative dietary interventions to optimize the prevention and management of overweight and obesity, while also improving the risk profile of these individuals. Trial registration ClinicalTrials.gov NCT05941871. Registered on 18 May 2023.
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- 2024
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12. Human colorectal cancer: upregulation of the adaptor protein Rai in TILs leads to cell dysfunction by sustaining GSK-3 activation and PD-1 expression
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Montecchi, Tommaso, Nannini, Giulia, De Tommaso, Domiziana, Cassioli, Chiara, Coppola, Federica, Ringressi, Maria Novella, Carraro, Fabio, Naldini, Antonella, Taddei, Antonio, Marotta, Giuseppe, Amedei, Amedeo, Baldari, Cosima T., and Ulivieri, Cristina
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- 2024
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13. Changes in cytokine and sequestosome-1 levels during twin pregnancy progression: Association with outcome
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Silvano, Angela, Sisti, Giovanni, Seravalli, Viola, Strambi, Noemi, Parenti, Astrid, Amedei, Amedeo, Witkin, Steven S., and Di Tommaso, Mariarosaria
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- 2024
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14. Oral microbiota signatures associated with viremia and CD4 recovery in treatment-naïve HIV-1-infected patients
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Nannini, Giulia, Di Gloria, Leandro, Russo, Edda, Sterrantino, Gaetana, Kiros, Seble Tekle, Coppi, Marco, Niccolai, Elena, Baldi, Simone, Ramazzotti, Matteo, Di Pilato, Vincenzo, Lagi, Filippo, Bartolucci, Gianluca, Rossolini, Gian Maria, Bartoloni, Alessandro, and Amedei, Amedeo
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- 2024
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15. Comparison of ELISA with automated ECLIA for IL-6 determination in COVID-19 patients: An Italian real-life experience
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Francesca Romano, Luisa Lanzilao, Edda Russo, Maria Infantino, Francesca Nencini, Giovanni Cappelli, Stefano Dugheri, Mariangela Manfredi, Alessandra Fanelli, Amedeo Amedei, and Nicola Mucci
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COVID-19 ,Interleukin-6 ,ELISA ,ECLIA ,Medicine (General) ,R5-920 ,Chemistry ,QD1-999 - Abstract
Objectives: Coronavirus disease 2019 (COVID-19) has a wide spectrum of clinical severity. A cytokine storm is associated with COVID-19 severity. Of these, IL-6 is significantly associated with higher mortality and is also a marker for predicting disease prognosis. IL-6 may act as a target for therapeutics and, a blockade of IL-6 function by Tocilizumab has been described as a treatment of the inflammatory process COVID-19-related. This study aims to describe our experience comparing two different methods, in detail Human IL-6 Instant ELISA and the Elecsys IL-6 based on ECLIA, for the IL-6 assessment. Design and methods: IL-6 levels from serum samples of 104 COVID-19 patients, admitted to the AOU Careggi (Hospital in Florence -Italy), were assessed by using the two above-mentioned methods, and the results were analysed through Passing-Bablok regression fit and Bland-Altman plot. Results: The regression exhibited a linear relation between the methods with a regression equation (y = - 0.13 + 0.63 x; 95 % C.I. intercept = − 0.13 to 4.55; 95 % C.I. slope = 1.03 to 1.26 with R2 = 0.89, p > 0.05), showing a positive slope. The agreement of the two methods reported a bias of −25.0 pg/mL. Thus, the two methods correlate but do not agree in terms of numeric results. Conclusions: The two assays showed good comparability. However, because of the extremely wide linear range of the ECLIA, its throughput and its capacity for immune profiling, it represents an interesting emerging technology in the immunology field.
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- 2024
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16. Potential Association of the Oral Microbiome with Trimethylamine N-Oxide Quantification in Mexican Patients with Myocardial Infarction
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Paulina Hernández-Ruiz, Alma R. Escalona Montaño, Luis M. Amezcua-Guerra, Héctor González-Pacheco, Elena Niccolai, Amedeo Amedei, and María M. Aguirre-García
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Pathology ,RB1-214 - Abstract
Many attempts have been proposed to evaluate the linkage between the oral–gut–liver axis and the mechanisms related to the diseases’ establishment. One of them is the oral microbiota translocation into the bloodstream, liver, and gut, promoting a host dysbiosis and triggering the presence of some metabolites such as trimethylamine N-oxide (TMAO), known as a risk marker for cardiovascular disease, and especially the myocardial infarction (MI). In the present pilot study, the involvement of oral dysbiosis related to the presence of TMAO has been considered an independent component of the standard risk factors (SRs) in the development of MI, which has not been previously described in human cohorts. A positive and significant correlation of TMAO levels with Porphyromonas was identified; likewise, the increase of the genus Peptidiphaga in patients without SRs was observed. We determined that the presence of SRs does not influence the TMAO concentration in these patients. This report is the first study where the relationship between oral dysbiosis and TMAO is specified in the Mexican population. Our findings provide information on the possible contribution of the oral pathogens associated with gut dysbiosis in the development of MI, although further analysis should be performed.
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- 2024
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17. Cytokine and microbiota profiles in obesity-related hypertension patients
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María Magdalena Aguirre-García, Amedeo Amedei, Paulina Hernández-Ruiz, Ana Pamela Gómez-García, Elena Niccolai, Aura M. Moreno-Rodríguez, Sandra Pinto-Cardoso, Adriana Alviter-Plata, Alma R. Escalona-Montaño, Erick R. Ordaz-Robles, María del C. González-Salazar, Rashidi Springall Del Villar, Enrique A. Berrios-Bárcenas, and Nydia Ávila-Vanzzini
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human microbiome ,oral microbiota ,gut microbiota ,obese ,overweight ,high blood pressure ,Microbiology ,QR1-502 - Abstract
BackgroundSystemic arterial hypertension is linked to a heightened risk of cardiovascular diseases on a global scale. In Mexico, nearly half of adults in vulnerable conditions experience hypertension. Imbalance in the oral and intestinal microbiota composition has been observed in patients with hypertension, documented by a decrease of bacteria producing short-chain fatty acids, which play a critical role in blood pressure regulation.AimTo examine the cytokines’ profile and assess the characteristics of oral and gut microbiota in obesity-related hypertension in Mexican patients.MethodsA cross-sectional, observational, and analytical study was carried out. Twenty-two patients were categorized by their body mass index (BMI) as overweight and obese, and the diagnosis of primary hypertension. DNA from supragingival dental plaque and feces samples was used to carry out 16S rRNA sequencing. Additionally, 13 cytokines were quantified.ResultsIn the oral microbiota, Kluyvera was found to be significantly enriched in obese compared to overweight patients. Instead, the gut microbiota was dominated by Firmicutes. However, the correlation between certain genera and proinflammatory cytokines was noted.ConclusionThis exploratory study provides insights into the complex relationship between the oral and gut microbiota and their association with systemic inflammation in obesity-related hypertension.
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- 2024
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18. Gastrointestinal Cancers: What Is the Real Board of Microenvironment and the Role of Microbiota–Immunity Axis?
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Russo, Edda, Boem, Federico, Curini, Lavinia, Amedei, Amedeo, Rezaei, Nima, Series Editor, Ahmed, Atif A., Editorial Board Member, Aguiar, Rodrigo, Editorial Board Member, Ambrosio, Maria R., Editorial Board Member, Artac, Mehmet, Editorial Board Member, Augustine, Tanya N., Editorial Board Member, Bambauer, Rolf, Editorial Board Member, Bhat, Ajaz Ahmad, Editorial Board Member, Bertolaccini, Luca, Editorial Board Member, Bianchini, Chiara, Editorial Board Member, Cavic, Milena, Editorial Board Member, Chakrabarti, Sakti, Editorial Board Member, Cho, William C. S., Editorial Board Member, Czarnecka, Anna M., Editorial Board Member, Domingues, Cátia, Editorial Board Member, Eşkazan, A. Emre, Editorial Board Member, Fares, Jawad, Editorial Board Member, Fonseca Alves, Carlos E., Editorial Board Member, Fru, Pascaline, Editorial Board Member, Da Gama Duarte, Jessica, Editorial Board Member, García, Mónica C., Editorial Board Member, Gener, Melissa A.H., Editorial Board Member, Estrada Guadarrama, José Antonio, Editorial Board Member, Hargadon, Kristian M., Editorial Board Member, Holvoet, Paul, Editorial Board Member, Jurisic, Vladimir, Editorial Board Member, Kabir, Yearul, Editorial Board Member, Katsila, Theodora, Editorial Board Member, Kleeff, Jorg, Editorial Board Member, Liang, Chao, Editorial Board Member, Tan, Mei Lan, Editorial Board Member, Li, Weijie, Editorial Board Member, Prado López, Sonia, Editorial Board Member, Macha, Muzafar A., Editorial Board Member, Malara, Natalia, Editorial Board Member, Orhan, Adile, Editorial Board Member, Prado-Garcia, Heriberto, Editorial Board Member, Pérez-Velázquez, Judith, Editorial Board Member, Rashed, Wafaa M., Editorial Board Member, Sanguedolce, Francesca, Editorial Board Member, Sorrentino, Rosalinda, Editorial Board Member, Shubina, Irina Zh., Editorial Board Member, de Araujo, Heloisa Sobreiro Selistre, Editorial Board Member, Torres-Suárez, Ana Isabel, Editorial Board Member, Włodarczyk, Jakub, Editorial Board Member, Yeong, Joe Poh Sheng, Editorial Board Member, Toscano, Marta A., Editorial Board Member, Wong, Tak-Wah, Editorial Board Member, Yin, Jun, Editorial Board Member, and Yu, Bin, Editorial Board Member
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- 2023
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19. Metabolomics of Gastrointestinal Cancers
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Nannini, Giulia, Meoni, Gaia, Tenori, Leonardo, Amedei, Amedeo, Rezaei, Nima, Series Editor, Ahmed, Atif A., Editorial Board Member, Aguiar, Rodrigo, Editorial Board Member, Ambrosio, Maria R., Editorial Board Member, Artac, Mehmet, Editorial Board Member, Augustine, Tanya N., Editorial Board Member, Bambauer, Rolf, Editorial Board Member, Bhat, Ajaz Ahmad, Editorial Board Member, Bertolaccini, Luca, Editorial Board Member, Bianchini, Chiara, Editorial Board Member, Cavic, Milena, Editorial Board Member, Chakrabarti, Sakti, Editorial Board Member, Cho, William C. S., Editorial Board Member, Czarnecka, Anna M., Editorial Board Member, Domingues, Cátia, Editorial Board Member, Eşkazan, A. Emre, Editorial Board Member, Fares, Jawad, Editorial Board Member, Fonseca Alves, Carlos E., Editorial Board Member, Fru, Pascaline, Editorial Board Member, Da Gama Duarte, Jessica, Editorial Board Member, García, Mónica C., Editorial Board Member, Gener, Melissa A.H., Editorial Board Member, Estrada Guadarrama, José Antonio, Editorial Board Member, Hargadon, Kristian M., Editorial Board Member, Holvoet, Paul, Editorial Board Member, Jurisic, Vladimir, Editorial Board Member, Kabir, Yearul, Editorial Board Member, Katsila, Theodora, Editorial Board Member, Kleeff, Jorg, Editorial Board Member, Liang, Chao, Editorial Board Member, Tan, Mei Lan, Editorial Board Member, Li, Weijie, Editorial Board Member, Prado López, Sonia, Editorial Board Member, Macha, Muzafar A., Editorial Board Member, Malara, Natalia, Editorial Board Member, Orhan, Adile, Editorial Board Member, Prado-Garcia, Heriberto, Editorial Board Member, Pérez-Velázquez, Judith, Editorial Board Member, Rashed, Wafaa M., Editorial Board Member, Sanguedolce, Francesca, Editorial Board Member, Sorrentino, Rosalinda, Editorial Board Member, Shubina, Irina Zh., Editorial Board Member, de Araujo, Heloisa Sobreiro Selistre, Editorial Board Member, Torres-Suárez, Ana Isabel, Editorial Board Member, Włodarczyk, Jakub, Editorial Board Member, Yeong, Joe Poh Sheng, Editorial Board Member, Toscano, Marta A., Editorial Board Member, Wong, Tak-Wah, Editorial Board Member, Yin, Jun, Editorial Board Member, and Yu, Bin, Editorial Board Member
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- 2023
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20. Comparison of ELISA with automated ECLIA for IL-6 determination in COVID-19 patients: An Italian real-life experience
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Romano, Francesca, Lanzilao, Luisa, Russo, Edda, Infantino, Maria, Nencini, Francesca, Cappelli, Giovanni, Dugheri, Stefano, Manfredi, Mariangela, Fanelli, Alessandra, Amedei, Amedeo, and Mucci, Nicola
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- 2024
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21. Oxidative stress–induced fibrinogen modifications in liver transplant recipients: unraveling a novel potential mechanism for cardiovascular risk
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Gitto, Stefano, Fiorillo, Claudia, Argento, Flavia Rita, Fini, Eleonora, Borghi, Serena, Falcini, Margherita, Roccarina, Davide, La Delfa, Rosario, Lillo, Ludovica, Zurli, Tommaso, Forte, Paolo, Ghinolfi, Davide, De Simone, Paolo, Chiesi, Francesca, Ingravallo, Angelica, Vizzutti, Francesco, Aspite, Silvia, Laffi, Giacomo, Lynch, Erica, Petruccelli, Stefania, Carrai, Paola, Palladino, Simona, Sofi, Francesco, Stefani, Laura, Amedei, Amedeo, Baldi, Simone, Toscano, Arianna, Lau, Chloe, Marra, Fabio, and Becatti, Matteo
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- 2024
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22. Gut–Liver–Pancreas Axis Crosstalk in Health and Disease: From the Role of Microbial Metabolites to Innovative Microbiota Manipulating Strategies
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Giada Marroncini, Laura Naldi, Serena Martinelli, and Amedeo Amedei
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gut microbiota ,hormones ,metabolites ,endocrine pathology ,gut–liver–pancreas axis ,Biology (General) ,QH301-705.5 - Abstract
The functions of the gut are closely related to those of many other organs in the human body. Indeed, the gut microbiota (GM) metabolize several nutrients and compounds that, once released in the bloodstream, can reach distant organs, thus influencing the metabolic and inflammatory tone of the host. The main microbiota-derived metabolites responsible for the modulation of endocrine responses are short-chain fatty acids (SCFAs), bile acids and glucagon-like peptide 1 (GLP-1). These molecules can (i) regulate the pancreatic hormones (insulin and glucagon), (ii) increase glycogen synthesis in the liver, and (iii) boost energy expenditure, especially in skeletal muscles and brown adipose tissue. In other words, they are critical in maintaining glucose and lipid homeostasis. In GM dysbiosis, the imbalance of microbiota-related products can affect the proper endocrine and metabolic functions, including those related to the gut–liver–pancreas axis (GLPA). In addition, the dysbiosis can contribute to the onset of some diseases such as non-alcoholic steatohepatitis (NASH)/non-alcoholic fatty liver disease (NAFLD), hepatocellular carcinoma (HCC), and type 2 diabetes (T2D). In this review, we explored the roles of the gut microbiota-derived metabolites and their involvement in onset and progression of these diseases. In addition, we detailed the main microbiota-modulating strategies that could improve the diseases’ development by restoring the healthy balance of the GLPA.
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- 2024
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23. Yin and Yang of Gut Microbiota in Cocaine Abuse
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Simone Baldi, Elisabetta Gerace, Guido Mannaioni, and Amedeo Amedei
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cocaine ,gut microbiota ,substance use disorder ,cocaine use disorder ,gut-brain axis ,Biochemistry ,QD415-436 ,Biology (General) ,QH301-705.5 - Published
- 2024
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24. Endometriosis, Pain, and Related Psychological Disorders: Unveiling the Interplay among the Microbiome, Inflammation, and Oxidative Stress as a Common Thread
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Francesca Cuffaro, Edda Russo, and Amedeo Amedei
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endometriosis ,psychological disorders ,microbiome ,chronic pelvic pain ,inflammation ,oxidative stress ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Endometriosis (EM), a chronic condition in endometrial tissue outside the uterus, affects around 10% of reproductive-age women, significantly affecting fertility. Its prevalence remains elusive due to the surgical confirmation needed for diagnosis. Manifesting with a range of symptoms, including dysmenorrhea, dyschezia, dysuria, dyspareunia, fatigue, and gastrointestinal discomfort, EM significantly impairs quality of life due to severe chronic pelvic pain (CPP). Psychological manifestations, notably depression and anxiety, frequently accompany the physical symptoms, with CPP serving as a key mediator. Pain stems from endometrial lesions, involving oxidative stress, neuroinflammation, angiogenesis, and sensitization processes. Microbial dysbiosis appears to be crucial in the inflammatory mechanisms underlying EM and associated CPP, as well as psychological symptoms. In this scenario, dietary interventions and nutritional supplements could help manage EM symptoms by targeting inflammation, oxidative stress, and the microbiome. Our manuscript starts by delving into the complex relationship between EM pain and psychological comorbidities. It subsequently addresses the emerging roles of the microbiome, inflammation, and oxidative stress as common links among these abovementioned conditions. Furthermore, the review explores how dietary and nutritional interventions may influence the composition and function of the microbiome, reduce inflammation and oxidative stress, alleviate pain, and potentially affect EM-associated psychological disorders.
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- 2024
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25. Breast cancer: the first comparative evaluation of oncobiome composition between males and females
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Elena Niccolai, Simone Baldi, Giulia Nannini, Francesca Gensini, Laura Papi, Vania Vezzosi, Simonetta Bianchi, Lorenzo Orzalesi, Matteo Ramazzotti, and Amedeo Amedei
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Oncobiome ,FFPE ,Dimorphism ,Microbiota ,Breast cancer ,Gender ,Medicine ,Physiology ,QP1-981 - Abstract
Abstract Background Emerging evidence suggests that breast microbiota dysbiosis contributes to cancer initiation, progression, prognosis and treatment efficacy. Anyway, available data are referred only to female patients, and studies on males are completely missing. Male breast cancer (MBC) is 70–100 times less frequent, but the mortality rate adjusted to incidence is higher in men than in females. Currently, MBC diagnostic approaches and treatments have generally been extrapolated from the clinical experience gained in women, while few studies focus on characterizing male cancer biology. Taking into account the rising importance of the oncobiome field and the need of MBC targeted studies, we explored the breast cancer oncobiome of male and female patients. Methods 16S rRNA gene sequencing was performed in 20 tumor and 20 non-pathological adjacent FFPE breast tissues from male and female patients. Results We documented, for the first time, the presence of a sexually dimorphic breast-associated microbiota, here defined as “breast microgenderome”. Moreover, the paired analysis of tumor and non-pathological adjacent tissues suggests the presence of a cancer-associated dysbiosis in male patients, with surrounding tissue conserving a healthier microbiome, whereas in female patients, the entire breast tissue is predisposed to cancer development. Finally, the phylum Tenericutes, especially the genera Mesoplasma and Mycobacterium, could to be involved in breast carcinogenesis, in both sexes, deserving further investigation, not only for its role in cancer development but even as potential prognostic biomarker. Conclusions Breast microbiota characterization can enhance the understanding of male breast cancer pathogenesis, being useful for detection of new prognostic biomarkers and development of innovative personalized therapies, remarking the relevant gender differences.
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- 2023
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26. Editorial: Impact of coronavirus disease 2019 (COVID-19) pandemic on nosocomial infection
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Mingke Wang, Mahlagha Dehghan, Chunhui Li, Amedeo Amedei, and Alfonso J. Rodriguez-Morales
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COVID-19 ,SARS-CoV-2 ,nosocomial infection ,clinical characteristics ,outcome ,pathophysiologic mechanisms ,Medicine (General) ,R5-920 - Published
- 2023
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27. The first taxonomic and functional characterization of human CAVD-associated microbiota
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Lavinia Curini, Brunilda Alushi, Mary Roxana Christopher, Simone Baldi, Leandro Di Gloria, Pierluigi Stefano, Anna Laganà, Luisa Iannone, Herko Grubitzsch, Ulf Landmesser, Matteo Ramazzotti, Elena Niccolai, Alexander Lauten, and Amedeo Amedei
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aortic valve disease ,valve calcification ,microbiota ,immune response ,t cells ,Biology (General) ,QH301-705.5 - Abstract
Introduction: Calcific aortic valve disease (CAVD) is the most common heart valve disorder, defined by a remodeling multistep process: namely, valve fibrosis with its area narrowing, impaired blood flow, and final calcification phase. Nowadays, the only treatment is the surgical valve replacement. As for other cardiovascular diseases, growing evidence suggest an active role of the immune system in the calcification process that could be modulated by the microbiota. To address this point, we aimed to investigate and characterize, for the first time, the presence of a valve microbiota and associated immune response in human CAVD. Method: Calcified aortic valve (CAV) samples from twenty patients (11 from Germany and 9 from Italy) with diagnosis of severe symptomatic CAVD were used to assess the presence of infiltrating T cells, by cloning approach, and to characterize the valve microbiota, by 16S rRNA gene sequencing (NGS). Results: We documented the presence of infiltrating T lymphocytes, especially the T helper subset, in CAV samples. Moreover, we found a tissue-associated microbiota in freshly collected CAV samples, which was significantly different in Italian and German patients, suggesting potential correlation with other cardiovascular risk factors. Conclusion: The presence of microbiota in inflamed CAV samples represents the right trigger point to explain the valve calcification process, encouraging further studies to explore the potential link between bacteria and adaptive immune response and to define the critical role of local microbiota-immunity axis on CAVD development.
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- 2023
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28. Circadian rhythms, gut microbiota, and diet: Possible implications for health
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Lotti, Sofia, Dinu, Monica, Colombini, Barbara, Amedei, Amedeo, and Sofi, Francesco
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- 2023
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29. Metabolomics of Gastrointestinal Cancers
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Nannini, Giulia, primary, Meoni, Gaia, additional, Tenori, Leonardo, additional, and Amedei, Amedeo, additional
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- 2023
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30. Contributors
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Abdel-Maksoud, Mostafa A., primary, Akhtari, Maryam, additional, AlHammadi, Ayda, additional, Ali Almohssen, Amer, additional, Amedei, Amedeo, additional, Asnaashari, Kosar, additional, Aviles, Raul, additional, de Barros, Samar Freschi, additional, Benlidayı, İlke Coşkun, additional, Bettiol, Alessandra, additional, Bizzaro, Nicola, additional, Branco, Carlos Eduardo, additional, Broere, Femke, additional, Cinquanta, Luigi, additional, do Carmo Pereira Nunes, Maria, additional, dos Santos, Ana Caroline Melo, additional, dos Santos, Bárbara Rayssa Correia, additional, Dutra, Jean Carlos Vencioneck, additional, van Eden, Willem, additional, Emmi, Giacomo, additional, Essouma, Mickael, additional, Farhadi, Elham, additional, Ferreira, Jean Moisés, additional, Frech, Michael, additional, Gorodetskiy, Vadim, additional, Guilherme, Luiza, additional, Hamzaoui, Agnès, additional, Hamzaoui, Kamel, additional, Houen, Gunnar, additional, Iyer, Janaki, additional, Jia, Hongxing, additional, Kalil, Jorge, additional, Khayambashi, Parisa, additional, Kwiatkowska, Dominika, additional, van Laar, Jacob M., additional, de Lima Filho, José Luiz, additional, Mahmoudi, Mahdi, additional, Mak, Anselm, additional, Mattioli, Irene, additional, de Moura, Edilson Leite, additional, Niccolai, Elena, additional, O’Neill, Sean, additional, Omata, Yasunori, additional, Oo, Win Min, additional, Perry, Martin E., additional, Prisco, Domenico, additional, Randone, Silvia Bellando, additional, Reich, Adam, additional, Rezaei, Nima, additional, Sakkas, Lazaros I., additional, Savage, Blake, additional, Schett, Georg, additional, Shaharir, Syahrul Sazliyana, additional, Simopoulou, Theodora, additional, Smith, Nicole Marie, additional, de Souza Figueiredo, Elaine Virgínia Martins, additional, Stoppelenburg, Arie J., additional, Talotta, Rossella, additional, Tanabe, Ithallo Sathio Bessoni, additional, Tanaka, Sakae, additional, Teixeira, Antonio Lucio, additional, Tozzoli, Renato, additional, Tran, Simon D., additional, Trier, Nicole Hartwig, additional, Turnbull, Katie S., additional, Vasconcelos, Luiz Paulo Bastos, additional, Vasconcelos, Marcelle Cristina, additional, Visser, H. John, additional, Wahab, Asrul Abdul, additional, Wolfe, Joshua, additional, Yazdanpanah, Niloufar, additional, Zaiss, Mario M., additional, and Zhang, Peipei, additional
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- 2023
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31. Rheumatic diseases: The microbiota-immunity axis in development and treatment
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Niccolai, Elena, primary, Randone, Silvia Bellando, additional, and Amedei, Amedeo, additional
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- 2023
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32. The Impact of Microbiota–Immunity–Hormone Interactions on Autoimmune Diseases and Infection
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Serena Martinelli, Giulia Nannini, Fabio Cianchi, Francesco Coratti, and Amedeo Amedei
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autoimmune diseases ,infections ,microbiota ,hormones ,immune system ,Biology (General) ,QH301-705.5 - Abstract
Autoimmune diseases are complex multifactorial disorders, and a mixture of genetic and environmental factors play a role in their onset. In recent years, the microbiota has gained attention as it helps to maintain host health and immune homeostasis and is a relevant player in the interaction between our body and the outside world. Alterations (dysbiosis) in its composition or function have been linked to different pathologies, including autoimmune diseases. Among the different microbiota functions, there is the activation/modulation of immune cells that can protect against infections. However, if dysbiosis occurs, it can compromise the host’s ability to protect against pathogens, contributing to the development and progression of autoimmune diseases. In some cases, infections can trigger autoimmune diseases by several mechanisms, including the alteration of gut permeability and the activation of innate immune cells to produce pro-inflammatory cytokines that recruit autoreactive T and B cells. In this complex scenario, we cannot neglect critical hormones’ roles in regulating immune responses. Different hormones, especially estrogens, have been shown to influence the development and progression of autoimmune diseases by modulating the activity and function of the immune system in different ways. In this review, we summarized the main mechanisms of connection between infections, microbiota, immunity, and hormones in autoimmune diseases’ onset and progression given the influence of some infections and hormone levels on their pathogenesis. In detail, we focused on rheumatoid arthritis, multiple sclerosis, and systemic lupus erythematosus.
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- 2024
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33. From adenoma to CRC stages: the oral-gut microbiome axis as a source of potential microbial and metabolic biomarkers of malignancy
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Russo, Edda, Gloria, Leandro Di, Nannini, Giulia, Meoni, Gaia, Niccolai, Elena, Ringressi, Maria Novella, Baldi, Simone, Fani, Renato, Tenori, Leonardo, Taddei, Antonio, Ramazzotti, Matteo, and Amedei, Amedeo
- Published
- 2023
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34. Oral and fecal microbiota perturbance in cocaine users: Can rTMS-induced cocaine abstinence support eubiosis restoration?
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Gerace, Elisabetta, Baldi, Simone, Salimova, Maya, Di Gloria, Leandro, Curini, Lavinia, Cimino, Virginia, Nannini, Giulia, Russo, Edda, Pallecchi, Marco, Ramazzotti, Matteo, Bartolucci, Gianluca, Occupati, Brunella, Lanzi, Cecilia, Scarpino, Maenia, Lanzo, Giovanni, Grippo, Antonello, Lolli, Francesco, Mannaioni, Guido, and Amedei, Amedeo
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- 2023
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35. Circulating Calprotectin (cCLP) in autoimmune diseases
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Manfredi, Mariangela, Van Hoovels, Lieve, Benucci, Maurizio, De Luca, Riccardo, Coccia, Carmela, Bernardini, Pamela, Russo, Edda, Amedei, Amedeo, Guiducci, Serena, Grossi, Valentina, Bossuyt, Xavier, Perricone, Carlo, and Infantino, Maria
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- 2023
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36. JAK inhibitors and autoimmune rheumatic diseases
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Benucci, Maurizio, Bernardini, Pamela, Coccia, Carmela, De Luca, Riccardo, Levani, Juela, Economou, Alessio, Damiani, Arianna, Russo, Edda, Amedei, Amedeo, Guiducci, Serena, Bartoloni, Elena, Manfredi, Mariangela, Grossi, Valentina, Infantino, Maria, and Perricone, Carlo
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- 2023
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37. SARS-CoV-2 and Microbiota
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Russo, Edda, Curini, Lavinia, Fabbrizzi, Alessio, Amedei, Amedeo, Gupta, Gaurav, editor, Oliver, Brian G., editor, Dua, Kamal, editor, Singh, Alisha, editor, and MacLoughlin, Ronan, editor
- Published
- 2022
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38. A comparative study of carbonic anhydrase activity in lymphocytes from colorectal cancer tissues and adjacent healthy counterparts
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Giulia Nannini, Viviana De Luca, Chiara D’Ambrosio, Andrea Scaloni, Antonio Taddei, Maria Novella Ringressi, Fabio Cianchi, Fabio Staderini, Clemente Capasso, Amedeo Amedei, and Claudiu T. Supuran
- Subjects
Carbonic anhydrase ,isoforms ,T cells ,tumour-infiltrating lymphocyte ,colorectal cancer ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Several carbonic anhydrase (CA, EC 4.2.1.1) isoforms play an essential role in processes connected to tumorigenesis, as they efficiently accelerate the hydration of carbon dioxide to bicarbonate and proton. In this context, examples are CA IX and CA XII, which were proved to be upregulated in many solid malignancies. On the other hand, cancer and the immune system are inextricably linked, and targeting the immune checkpoints recently was shown to efficiently improve the treatment of malignancies. In this study, we have investigated the expression of CA isoforms in tumour-infiltrating lymphocytes (TILs) that, according to the immunosurveillance theory, were suggested to have a crucial role in the development of colorectal cancer (CRC). T lymphocytes isolated from healthy surrounding mucosa showed a higher CA activity compared to those present in tumour and peripheral blood in the same patients. CA I and II were confirmed as enzyme isoforms involved in the process, as determined by proteomic analysis of corresponding TIL samples. These preliminary findings suggest a dysregulation of the local immune response in the CRC tissues and a loss of effective anticancer mechanisms mediated by CAs therein.
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- 2022
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39. Musculin does not modulate the disease course of Experimental Autoimmune Encephalomyelitis and DSS colitis
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Vanni, Anna, Carnasciali, Alberto, Mazzoni, Alessio, Russo, Edda, Farahvachi, Parham, Gloria, Leandro Di, Ramazzotti, Matteo, Lamacchia, Giulia, Capone, Manuela, Salvati, Lorenzo, Calosi, Laura, Bani, Daniele, Liotta, Francesco, Cosmi, Lorenzo, Amedei, Amedeo, Ballerini, Clara, Maggi, Laura, and Annunziato, Francesco
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- 2023
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40. Nutrients, foods and dietary patterns in the management of autoimmune rheumatic diseases
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Giuditta Pagliai, Barbara Colombini, Silvia Bellando Randone, Amedeo Amedei, Serena Guiducci, and Francesco Sofi
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Diet ,Rheumatic disease ,Arthritis ,Nutrition ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Summary: Background: Rheumatic disease (RD) represents a broad spectrum of systemic conditions characterized by inflammation and pain in muscles or joints with a significant burden on quality of life. Increasing evidence suggests that diet could play a modulatory role in RD by influencing cardiovascular diseases (CVD) risk factors frequently present in these patients as well as inflammation and antioxidant defence. Objectives: This review aims to summarize the available evidence on the effect of nutrients, foods and dietary patterns on the most common autoimmune inflammatory RD including rheumatoid arthritis, Sjogren's syndrome, systemic lupus erythematosus and systemic sclerosis. Results: We documented that MUFAs and PUFAs seem to have positive effects in modulating the inflammatory process. Regarding the dietary interventions, low-calorie diets, Mediterranean diet and fasting appear to be effective in reducing the symptoms of the most common RD. Positive results were also obtained in some cases with gluten-free, low-fat, vegan, elimination or anti-inflammatory diets. Conclusion: Although further and specific studies are needed, the fact that people obtained an improvement in clinical outcomes after almost all these dietary patterns suggests that a healthy diet could play a pivotal role in the RD management.
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- 2022
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41. From adenoma to CRC stages: the oral-gut microbiome axis as a source of potential microbial and metabolic biomarkers of malignancy
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Edda Russo, Leandro Di Gloria, Giulia Nannini, Gaia Meoni, Elena Niccolai, Maria Novella Ringressi, Simone Baldi, Renato Fani, Leonardo Tenori, Antonio Taddei, Matteo Ramazzotti, and Amedeo Amedei
- Subjects
Microbiota ,colorectal cancer ,colonic adenomatous polyps ,microbiota ,metabolomics ,biomarkers ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Approximately 95% of Colorectal cancers (CRC) consist of adenocarcinomas originating from colonic Adenomatous polyps (AP). Increasing importance in CRC occurrence and progression has been attributed to the gut microbiota; however, a huge proportion of microorganisms inhabit the human digestive system. So, to comprehensively study the microbial spatial variations and their role in CRC progression, from AP to the different CRC phases, a holistic vision is imperative, including the simultaneous evaluation of multiple niches from the gastrointestinal system. Through an integrated approach, we identified potential microbial and metabolic biomarkers, able to discriminate human CRC from AP and/or also the different Tumor node metastasis (TNM) staging. In addition, as the microbiota contributes to the production of essential metabolic products detectable in fecal samples, we analysed and compared metabolites obtained from CRC and AP patients by using a Nuclear magnetic resonance (NMR) approach. Methods: In this observational study, saliva, tissue and stool samples from 61 patients, have been collected, including 46 CRC and 15 AP patients, age and sex-matched, undergoing surgery in 2018 at the Careggi University Hospital (Florence, Italy). First, the microbiota in the three-district between CRC and AP patients has been characterized, as well as in different CRC TNM stages. Subsequently, proton NMR spectroscopy has been used in combination with multivariate and univariate statistical approaches, to define the fecal metabolic profile of a restricted group of CRC and AP patients. Results: CRC patients display a different profile of tissue and fecal microbiota with respect to AP patients. Significant differences have been observed in CRC tissue microbial clades, with a rise of the Fusobacterium genus. In addition, significant taxa increase at the genus level has been observed in stool samples of CRC patients. Furthermore, Fusobacterium found in intestinal tissue has been positively correlated with fecal Parvimonas, for the first time. Moreover, as predicted by metagenomics pathway analysis, a significant increase of lactate (p=0.037) has been observed in the CRC fecal metabolic profiles, and positively correlated with Bifidobacterium (p=0.036). Finally, minor bacterial differences in CRC patients at stage T2 (TNM classification) have been detected, with a raise of the Spirochaetota phylum in CRC samples, with a slight increase of the Alphaproteobacteria class in fecal samples. Conclusion: Our results suggest the importance of microbiota communities and oncometabolites in CRC development. Further studies on CRC/AP management with a focus on CRC assessment are needed to investigate novel microbial-related diagnostic tools aimed to improve therapeutic interventions.
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- 2023
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42. POS0044 DECIPHERING THE GUT MICROBIOTA OF VERY EARLY SYSTEMIC SCLEROSIS (VEDOSS) PATIENTS: A TAXONOMIC AND FUNCTIONAL CHARACTERIZATION
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Russo, E., primary, Lepri, G., additional, Baldi, S., additional, Fioretto, B. S., additional, Romano, E., additional, El Aoufy, K., additional, Ramazzotti, M., additional, Rosa, I., additional, Ghezzi, G., additional, Gloria, L. DI, additional, Orlandi, M., additional, Bertorello, S., additional, Bruni, C., additional, Guiducci, S., additional, Manetti, M., additional, Matucci-Cerinic, M., additional, Amedei, A., additional, and Bellando Randone, S., additional
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- 2024
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43. ’Ho qui trovato un nuovo modo di dipingere’: pittrici finlandesi nel centro Italia fra Ottocento e Novecent
- Author
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Michele Amedei
- Subjects
Italia di mezzo ,Finlandia ,Toscana ,Artiste finlandesi ,Macchiaioli ,Espressionismo ,Avanguardie ,Language and Literature ,Literature (General) ,PN1-6790 - Abstract
L’articolo indaga il soggiorno nel centro Italia di un gruppo di influenti pittrici finlandesi fra Ottocento e Novecento. Residenti a Firenze in momenti diversi della loro vita, le artiste esplorarono zone dell’entroterra e della costa toscana fuori delle più tradizionali mete turistiche, nel momento in cui in Finlandia andava maturandosi un rinnovato interesse per l’Italia, per la sua cultura e soprattutto per le antiche tradizioni pittoriche medievali e rinascimentali. Il soggiorno di quelle donne in Italia fu dunque segnato dallo studio dell’arte del passato, dall’esercizio della copia, dall’esplorazione delle campagne toscane, reinterpretate attraverso un registro che preferisce al più consolidato stile “pittoresco” lo sperimentalismo linguistico dedotto dal confronto con l’arte locale ma anche francese e tedesca contemporanea. I risultati sono dipinti e disegni, conservati nella maggior parte dei casi presso i principali siti museali finlandesi, eseguiti nel solco di quella sensibilità vitalistica nordica alla base della quale è l’adorazione della bellezza mediterranea nonché la fiducia nel ritorno della Classicità nella civiltà Occidentale.
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- 2023
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44. Editorial: The mechanism of immune cells in the development of inflammatory bowel disease (IBD) and colitis-associated colorectal cancer (CAC)
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Edda Russo, Kai Yin, Xiumei Sheng, Fei Mao, and Amedeo Amedei
- Subjects
immunology & inflammation ,inflammatory bowel disease ,colitis associated cancer (CAC) ,immune cell ,immunotherapy ,Immunologic diseases. Allergy ,RC581-607 - Published
- 2023
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45. Corrigendum: Characterization of the 'gut microbiota-immunity axis' and microbial lipid metabolites in atrophic and potential celiac disease
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Federica Ricci, Edda Russo, Daniela Renzi, Simone Baldi, Giulia Nannini, Gabriele Lami, Marta Menicatti, Marco Pallecchi, Gianluca Bartolucci, Elena Niccolai, Matteo Cerboneschi, Serena Smeazzetto, Matteo Ramazzotti, Amedeo Amedei, and Antonino Salvatore Calabrò
- Subjects
potential celiac disease ,celiac disease ,microbiota ,immune response ,fatty acids ,T cells ,Microbiology ,QR1-502 - Published
- 2023
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46. Metadherin-driven promotion of cancer stem cell phenotypes and its effect on immunity in hepatocellular carcinoma
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Todorović, Nevena, primary and Amedei, Amedeo, additional
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- 2024
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47. mini-Complexome Profiling (mCP), an FDR-controlled workflow for global targeted detection of protein complexes
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Amedei, Hugo, primary, Paul, Niels, additional, Foo, Brian, additional, Neuenroth, Lisa, additional, Lehnart, Stephan E., additional, Urlaub, Henning, additional, and Lenz, Christof, additional
- Published
- 2024
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48. Effects of the probiotic Lactiplantibacillus plantarum IMC 510® on body composition, biochemical parameters, gut microbiota composition and function, and clinical symptoms of overweight/obese subjects
- Author
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Giuditta Pagliai, Maria Magdalena Coman, Simone Baldi, Monica Dinu, Giulia Nannini, Edda Russo, Lavinia Curini, Barbara Colombini, Sofia Lotti, Marco Pallecchi, Leandro Di Gloria, Gianluca Bartolucci, Matteo Ramazzotti, Maria Cristina Verdenelli, Francesco Sofi, and Amedeo Amedei
- Subjects
Lactiplantibacillus plantarum IMC 510® ,probiotic supplementation ,gut microbiota ,obesity ,clinical trial ,Nutrition. Foods and food supply ,TX341-641 - Abstract
Background and aimIn recent decades, obesity prevalence has reached epidemic proportions and considering the pivotal role of gut microbiota (GM) in the regulation of energy balance, alternative non-pharmacological approaches involving probiotics’ administration have been proposed. The aim of the present study was to evaluate the effect of Lactiplantibacillus plantarum IMC 510® supplementation on anthropometric and biochemical parameters, GM composition and functionality, and gastrointestinal and general symptoms of overweight/obese subjects.MethodsForty overweight/obese subjects were randomly assigned to daily consume the probiotic Lactiplantibacillus plantarum IMC 510® or placebo for 3 months. Before and after the administration period, anthropometric and biochemical parameters, self-administered questionnaires, and plasma and stool samples were obtained from each participant. The GM characterization was performed with 16S rRNA sequencing, while fecal short (SCFAs) and medium (MCFAs) chain fatty acids were analyzed with a gas chromatography–mass spectrometry protocol.ResultsCompared to placebo, probiotic supplementation determined a significant decrease in body weight, BMI, waist circumference, waist-to-height ratio, and blood glucose. Moreover, probiotic administration produced a significant decrease of the genera Hafnia-Obesumbacterium and Romboutsia and an increase of Succiniclasticum spp.; conversely, placebo administration resulted in the decrease of Actinomycetaceae and an increase of both Alloprevotella spp. and of the levels of pro-inflammatory hexanoic and heptanoic acids.ConclusionThanks to its effect in increasing some beneficial gut bacteria and lowering effects on waist circumference, fasting glucose levels and gastrointestinal symptoms of obese subjects, Lactiplantibacillus plantarum IMC 510® supplementation could represent a future and encouraging strategy for the prevention or treatment of obesity.
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- 2023
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49. Adverse Effects of Micro- and Nanoplastics on Humans and the Environment
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Elena Niccolai, Ilaria Colzi, and Amedeo Amedei
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n/a ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The pervasive pollution caused by nano- and microplastics (N/MPLs) is a pressing concern, and was exacerbated during the COVID-19 pandemic due to the substantial release of disposable Personal Protective Equipment (PPE) into the environment [...]
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- 2023
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50. Comparative characterization of inflammatory profile and oral microbiome according to an inflammation-based risk score in ST-segment elevation myocardial infarction
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Paulina Hernández-Ruiz, Luis M. Amezcua-Guerra, Yolanda López-Vidal, Héctor González-Pacheco, Sandra Pinto-Cardoso, Amedeo Amedei, and María Magdalena Aguirre-García
- Subjects
oral microbiome ,inflammatory markers ,STEMI ,cardiac risk ,cytokines ,Microbiology ,QR1-502 - Abstract
Ischemic heart disease considers the myocardial infarction (MI), either non-ST-segment elevation (non-STEMI) or ST-segment elevation myocardial infarction (STEMI); this represents the main cause of mortality in Mexican population. Regarding to the inflammatory state, this is reported to be a major prognostic factor of mortality for patients with MI. One of the conditions capable of producing systemic inflammation is periodontal disease. It has been proposed that the oral microbiota is translocated through the bloodstream to the liver and intestine, generating intestinal dysbiosis. The aim of this protocol is to assess oral microbiota diversity and circulating inflammatory profile in STEMI patients stratified according to an inflammation-based risk scoring system. We found that Bacteriodetes phylum was the most abundant in STEMI patients, and Prevotella was the most abundant genus, with a higher proportion in periodontitis patients. In fact, Prevotella genus was found to correlate positively and significantly with elevated IL-6 concentration. Our study defined a non-causal association inferred between the cardiovascular risk of STEMI patients, determined by changes in the oral microbiota that influence the development of periodontal disease and its relationship with the exacerbation of the systemic inflammatory response.
- Published
- 2023
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