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We report a genome sequence of Wohlfahrtiimonas chitiniclastica strain MUWRP0946, isolated from a hospitalized patient in Uganda. The genome size was 2.08 million bases, and the genome completeness was 94.22%. The strain carries tetracycline, folate pathway antagonist, β-lactam, and aminoglycoside antibiotic resistance genes.

Third-dose coronavirus disease 2019 vaccines are being deployed widely but their efficacy has not been assessed adequately in vulnerable older people who exhibit suboptimal responses after primary vaccination series. This observational study, which was carried out by the VIVALDI study based in England, looked at spike-specific immune responses in 341 staff and residents in long-term care facilities who received an mRNA vaccine following dual primary series vaccination with BNT162b2 or ChAdOx1. Third-dose vaccination strongly increased antibody responses with preferential relative enhancement in older people and was required to elicit neutralization of Omicron. Cellular immune responses were also enhanced with strong cross-reactive recognition of Omicron. However, antibody titers fell 21–78% within 100 d after vaccine and 27% of participants developed a breakthrough Omicron infection. These findings reveal strong immunogenicity of a third vaccine in one of the most vulnerable population groups and endorse an approach for widespread delivery across this population. Ongoing assessment will be required to determine the stability of immune protection.

Current trends in the automotive industry towards engine downsizing means turbocharging now plays a vital role in engine performance. The purpose of turbocharging is to increase the engine inlet air density by utilising, the otherwise wasted energy in the exhaust gas. This energy extraction is commonly accomplished through the use of a radial turbine. Although less commonly used, mixed flow turbines can offer aerodynamic advantages due to the manipulation of blade leading (LE) angles, improving performance at low velocity ratios. The current paper investigates the performance of a mixed flow turbine with four volute designs, two radial and two tilted volutes each with one variant with an aspect ratio (AR)=0.5 and one with AR = 2. To ensure constant mass flow parameter (MFP) for aerodynamic similarity, volute area to radius ratio (A/r) was manipulated between the design variants. The maximum variation of cycle averaged normalized efficiency measured between the designs was 2.87%. Purely in the rotor region, the variation in normalized cycle averaged efficiency was 3%. The smallest volute AR designs showed substantial secondary flow development. The introduction of volute tilt further complicated the secondary flow development with the introduction of asymmetry to the flows. It was established that both AR and tilt have a notable effect on secondary flows, rotor inlet conditions and over all mixed flow turbine performance.

Dyes acquired from diverse natural sources are biodegradable and require minimal use of chemicals during extraction which makes them appropriate alternatives to synthetic dyes which are non-biodegradable and toxic to the environment. In this research optimization of extraction conditions of natural dye extract from Euclea divinorium was conducted using Response Surface Methodology (RSM). M:L (material to liquor ratio), temperature and time were optimised using methanol and water as the extraction solvents. The combined effects of the investigated extraction conditions on absorbance at maximum wavelength of 339nm 341nm for aqueous and methanolic extractions, respectively, were studied using Central Composite Design (CCD) of experiment. Analysis of variance (ANOVA) was used to evaluate the efficiency of the statistical model fitted to the experimental design. The optimum values for aqueous dye extraction were found to be 7.5g:100mL, 84C and 146 minutes while the optimum values for methanolic dye extraction were found to be 7.5g:100mL, 60C and 129 minutes with absorbance values of 1.051 and 1.092, respectively. The theoretical and the experimental optimum absorbance verified experimentally were in agreement hence the statistical model was found to be valid.

[EN] Developments in materials, manufacturing and computing methods have catalysed the generation of efficient compressor designs with higher specific power outputs. Centrifugal compressors have become pervasive in environments demanding a combination of higher power with smaller sizes such as unmanned aerial vehicles, micro gas turbines and turbochargers. These compressors are expected to perform optimally in a range of operational speeds and mass flow states with low acoustic emissions. The impact of operating speed on the flow and acoustic characteristics of a ported shroud compressor has been explored in this work. The operation of the open and blocked configurations of the compressor at the design and near surge points each of a lower and a higher speedline was numerically and experimentally investigated. Comparing the results, the model was shown to predict the operation of the compressor for both configurations at the investigated operating points satisfactorily in terms of both performance and dominant acoustic features. With an increase in the velocity and the Mach number due to increased operational speed, changes in the flow behaviour in the inducer and diffuser were observed. An increase in operational speed was shown to generally increase the overall acoustic emission of the compressor for both configurations. The number of distinct tones in the acoustic output and their magnitude were also seen to be a function of operating speed., The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The project was sponsored and supported by the BorgWarner Turbo Systems and the Regional Growth Fund (RGF Grant Award 01.09.07.01/1789C).

Albizia coriaria Welw ex. Oliver is a customary African medicinal plant, which has a long history of utilization in the management of oxidative stress-induced and bacterial diseases. However, there is no report on the phytochemicals, antioxidant, and antibacterial activities of its leaves. The aim of this study was therefore to compare the phytochemicals, antioxidant, and antibacterial potential of A. coriaria leaves from Jinja, Kole, and Mbarara districts of Uganda. Shade-dried leaf samples were ground into powder and successively extracted with ethyl acetate, ethanol, and distilled water. Phytochemical screening indicated the presence of alkaloids, phenols, saponins, flavonoids, cardiac glycosides, tannins, and terpenes as the major secondary metabolites in the extracts. Total phenolic and flavonoid contents and total in vitro antioxidant activity were found to be the highest for ethanolic extracts, with the highest contents (101.72 ± 0.22 mg GAE/g DW; 13.23 ± 0.03 mg QE/g DW) and antioxidant potential (IC50 = 18.65 ± 0.06 mg/mL) being for leaves from Mbarara district. Antibacterial activity of the extracts determined by agar disc diffusion method revealed that ethanolic extracts had higher antibacterial activities with mean zones of inhibition of 6.00 ± 1.73 to 10.00 ± 1.73 mm, 5.00 ± 1.00 to 12.30 ± 1.53 mm, 17.00 ± 0.00 to 25.00 ± 2.65 mm, and 9.00 ± 1.73 to 16.00 ± 1.73 mm for Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella typhi, respectively. Ethyl acetate extracts of A. coriaria leaves from Kole and Mbarara had lower antibacterial activities, while aqueous extracts and ethyl acetate extract of leaves from Jinja showed no antibacterial activity. The current study for the first time established that A. coriaria leaves possess therapeutic phytochemicals with significant in vitro antioxidant and antibacterial activities, which lend credence to their use in traditional management of oxidative stress-induced conditions and bacterial diseases in Uganda. Structural elucidation of the responsible pure compounds for the observed bioactivities as well as toxicity studies of the extracts is recommended.

Aflatoxins are endemic in Kenya. The 2004 outbreak of acute aflatoxicosis in the country was one of the unprecedented epidemics of human aflatoxin poisoning recorded in mycotoxin history. In this study, an elaborate review was performed to synthesize Kenya’s major findings in relation to aflatoxins, their prevalence, detection, quantification, exposure assessment, prevention, and management in various matrices. Data retrieved indicate that the toxins are primarily biosynthesized by Aspergillus flavus and A. parasiticus, with the eastern part of the country reportedly more aflatoxin-prone. Aflatoxins have been reported in maize and maize products (Busaa, chan’gaa, githeri, irio, muthokoi, uji, and ugali), peanuts and its products, rice, cassava, sorghum, millet, yams, beers, dried fish, animal feeds, dairy and herbal products, and sometimes in tandem with other mycotoxins. The highest total aflatoxin concentration of 58,000 μg/kg has been reported in maize. At least 500 acute human illnesses and 200 deaths due to aflatoxins have been reported. The causes and prevalence of aflatoxins have been grossly ascribed to poor agronomic practices, low education levels, and inadequate statutory regulation and sensitization. Low diet diversity has aggravated exposure to aflatoxins in Kenya because maize as a dietetic staple is aflatoxin-prone. Detection and surveillance are only barely adequate, though some exposure assessments have been conducted. There is a need to widen diet diversity as a measure of reducing exposure due to consumption of aflatoxin-contaminated foods.

[EN] Developments in computing infrastructure and methods over the last decade have enhanced the potential of numerical methods to reasonably predict the aerodynamic noise. The generation and propagation of the flow induced noise are aerodynamic phenomena. Although the fluid flow dynamics and the resultant acoustics are both governed by mass and momentum conservation equations, former is of convective and for diffusive nature while the latter is propagative showing insignificant attenuation due to viscosity except for small viscothermal losses. Aeroacoustic modelling of systems with intricate geometries and complex flow is still not mature due to challenges in the accurate tractable representation of turbulent viscous flows. Therefore, state-of-the-art for computing flow-induced noise in small centrifugal compressors is reviewed and critical evaluation of various parameters in the numerical model is undertaken in this work. The impact of various turbulence formulations along with corresponding spatial and temporal resolutions on performance and acoustic predictions are quantified. The performance predictions are observed to be within 1.5% of the measured values irrespective of turbulence and timestep parameters. The noise generated by the impeller is observed to be reasonably correlated with the measurements and the absolute values of the sound pressure levels along with decay rates predicted by LES and SBES formulations are better than the similar predictions from DES and URANS formulations. The impact of timestep size is observed and is determinant of the frequency up to which spectra can be appropriately resolved. Furthermore, results emphasise the importance of high spatial resolution for scale resolving turbulence formulations to yield better results and the information can be used to select appropriate numerical configuration considering time and accuracy trade-offs. (C) 2020 Elsevier Masson SAS. All rights reserved., The project is sponsored and supported by BorgWarner Turbo Systems and the Regional Growth Fund (RGF Grant Award 01.09.07.01/1789C). The authors would like to thank BorgWarner Turbo Systems for permission to publish the results presented in this paper. The support of the HPC group at the University of Huddersfield is gratefully acknowledged.

The burden of neoplastic diseases is a significant global health challenge accounting for thousands of deaths. In Uganda, about 32,617 cancer cases were reported in 2018, accompanied by 21,829 deaths. In a view to identify some potential anticancer plant candidates for possible drug development, the current study was designed to compile the inventory of plants with reported anticancer activity used in rural Uganda and the evidences supporting their use in cancer therapy. An electronic survey in multidisciplinary databases revealed that 29 plant species belonging to 28 genera distributed among 24 families have been reported to be used in the management of cancer in Uganda. Anticancer plants were majorly from the families Bignoniaceae (7%), Caricaceae (7%), Fabaceae (7%), Moraceae (7%), and Rutaceae (7%). Most species occur in the wild (52%), though some are cultivated (48%). The growth habit of the plants is as trees (55%) or herbs (45%). Anticancer extracts are usually prepared from leaves (29%), bark (24%), roots (21%), and fruits (13%) through decoctions (53%), as food spices (23%) or pounded to produce ointments that are applied topically (10%). Prunus africana (Hook.f.) Kalkman, Opuntia species, Albizia coriaria (Welw. ex Oliver), Daucus carota L., Cyperus alatus (Nees) F. Muell., Markhamia lutea (Benth.) K. Schum., and Oxalis corniculata L. were the most frequently encountered species. As per global reports, Allium sativum L., Annona muricata L., Carica papaya L., Moringa oleifera Lam., Opuntia species, Prunus africana (Hook.f.) Kalkman, and Catharanthus roseus (L.) G. Don. are the most studied species, with the latter having vincristine and vinblastine anticancer drugs developed from it. Prostate, cervical, breast, and skin cancers are the top traditionally treated malignancies. There is a need to isolate and evaluate the anticancer potential of the bioactive compounds in the unstudied claimed plants, such as Cyperus alatus (Nees) F. Muell., Ficus dawei Hutch., Ficus natalensis Hochst., and Lovoa trichilioides Harms, and elucidate their mechanism of anticancer activity.

[EN] In this article, the acoustic characterisation of a turbocharger compressor with ported shroud design is carried out through the numerical simulation of the system operating under design conditions of maximum isentropic efficiency. While ported shroud compressors have been proposed as a way to control the flow near unstable conditions in order to obtain a more stable operation and enhance deep surge margin, it is often assumed that the behaviour under stable design conditions is characterised by a smooth, non-detached flow that matches an equivalent standard compressor. Furthermore, research is scarce regarding the acoustic effects of the ported shroud addition, especially under the design conditions. To analyse the flow field evolution and its relation with the noise generation, spectral signatures using statistical and scale-resolving turbulence modelling methods are obtained after successfully validating the performance and acoustic predictions of the numerical model with experimental measurements. Propagation of the frequency content through the ducts has been estimated with the aid of pressure decomposition methods to enhance the content coming from the compressor. Expected acoustic phenomena such as `buzz-saw¿ tones, blade passing peaks and broadband noise are correctly identified in the modelled spectrum. Analysis of the flow behaviour in the ported shroud shows rotating structures through the slot that may impact the acoustic and vibration response. Further inspection of the pressure field through modal decomposition confirms the influence of the ported shroud cavity in noise generation and propagation, especially at lower frequencies, suggesting that further research should be carried out on the impact these flow enhancement solutions have on the noise emission of the turbocharger., The project was sponsored and supported by BorgWarner Turbo Systems and the Regional Growth Fund (RGF Grant Award 01.09.07.01/1789C). The authors would like to thank BorgWarner Turbo Systems for permission to publish the results presented in this article. The support of the HPC group at the University of Huddersfield is gratefully acknowledged.

[EN] With the advancements in manufacturing, materials and computational methods, turbomachinery designs have become more efficient providing higher specific power and reliability with lower weight and cost. The rotational speed of turbomachines has increased while the overall machine size has decreased for a similar power output leading to the pervasive presence of small, high-speed turbomachines, specifically centrifugal compressors in helicopters, unmanned aerial vehicles (UAVs), auxiliary power units (APUs), turbochargers and micro gas turbines. In addition to superior aerodynamic performance over a wide range, increased operating speeds have made the acoustic emissions of small centrifugal compressors a critical aspect of design. Therefore, this work presents an experimental campaign to characterise the acoustic behaviour of a compressor with an intent to quantify the dominant features of the flow-induced noise for design and near surge operating conditions. Furthermore, the campaign is extended to establish the impact of the ported shroud casing treatment and operating speed on the acoustic emission of the compressor. The in-duct noise measurement method is used in this work to quantify the noise generated in the compressor by measuring pressure fluctuations near the inducer and diffuser while the propagation of the generated noise to the ducts is computed from an array of piezoelectric sensors. Spectra at the design operating point are dominated by tonal noise while broadband noise content is the dominant feature of spectra for near surge operation. Although the ported shroud cavity does not significantly alter the overall noise levels of the compressor operating at design condition, it does seem to propagate tonal noise. For near surge operation, the casing treatment positively impacts the acoustic emission with a reduction of approximately 10 dB in the range up to the blade pass frequency. Furthermore, various broadband features are also observed to be alleviated by the casing treatment. (C) 2019 Elsevier Masson SAS. All rights, The project was sponsored and supported by BorgWarner Turbo Systems and the Regional Growth Fund (RGF Grant Award 01.09.07.01/1789C). The authors would like to thank BorgWarner Turbo Systems for permission to publish the results presented in this paper.

Current trends in the automotive industry towards engine downsizing means turbocharging now plays a vital role in engine performance. A turbocharger increases charge air density using a turbine to extract waste energy from the exhaust gas to drive a compressor. Most turbocharger applications employ a radial inflow turbine. However, to ensure radial stacking of the blade fibers and avoid excessive blade stresses, the inlet blade angle must remain at zero degrees, creating large incidence angles. Alternately, mixed flow turbines can offer non-zero blade angles while maintaining radial stacking of the blade fibers and reducing leading edge separation at low velocity ratios. Furthermore, the physical blade cone angle introduced reduces the blade mass at the rotor outer diameter reducing rotor inertia and improving turbine transient response. The current paper investigates the performance of a mixed flow turbine under a range of pulsating inlet flow conditions. A significant variation in incidence across the LE span was observed within the pulse, where the distribution of incidence over the LE span was also found to change over the duration of the pulse. Analysis of the secondary flow structures developing within the volute shows the non-uniform flow distribution at the volute outlet is the result of the Dean effect in the housing passage. In-depth analysis of the mixed flow effect is also included, showing that poor axial flow turning ahead of the rotor was evident, particularly at the hub, resulting in modest blade angles. This work shows that the complex secondary flow structures that develop in the turbine volute are heavily influenced by the inlet pulsating flow. In turn, this significantly impacts the rotor inlet conditions and rotor losses.