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7. Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.

Author

Vatanparast, Mohammad, Merkel, Lisa, and Amari, Khalid

Subjects
PLANT protection, DOUBLE-stranded RNA, RISK assessment, RNA interference, PESTICIDES, SMALL interfering RNA
Abstract

The use of double-stranded RNA (dsRNA) for plant protection shows great potential as a sustainable alternative to traditional pesticides. This review summarizes the current state of knowledge on using exogenous dsRNA in plant protection and includes the latest findings on the safety and efficiency of this strategy. The review also emphasizes the need for a cautious and comprehensive approach, considering safety considerations such as off-target effects and formulation challenges. The regulatory landscape in different regions is also discussed, underscoring the need for specific guidelines tailored to dsRNA-based pesticides. The review provides a crucial resource for researchers, regulators, and industry stakeholders, promoting a balanced approach incorporating innovation with thorough safety assessments. The continuous dialog emphasized in this review is essential for shaping the future of dsRNA-based plant protection. As the field advances, collaboration among scientists, regulators, and industry partners will play a vital role in establishing guidelines and ensuring the responsible, effective, and sustainable use of dsRNA in agriculture. [ABSTRACT FROM AUTHOR]

Published
2024
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13. The effect of calcium ions on the flotation behavior of fluorapatite.

Author

Derqaoui, Mohammed, Aarab, Imane, Abidi, Abdelmoughit, Yaacoubi, Abdelrani, El Amari, Khalid, Etahiri, Abderahman, and Baçaoui, Abdelaziz

Subjects
CALCIUM ions, FLUORAPATITE, ZETA potential, FLOTATION, CALCIUM alginate, SODIUM alginate, ALGINATES
Abstract

The flotation behavior of apatite, a main mineral source of phosphorus, is strongly affected by the dissolved ionic species from gangue minerals. In this study, the effect of calcium ions on the fluorapatite flotation using the extracted sodium alginate E.SA depressant was investigated through microflotation tests, adsorption experiments, zeta potential measurements, and FTIR analyses. Although the amount of NaOL collector adsorbed on fluorapatite increased with increasing calcium concentrations, microflotation results reveal that adding calcium ions could deteriorate the fluorapatite recovery. More significant depression of fluorapatite could be achieved when Ca2+ and E.SA co-existed in the system. Zeta potential measurements and FTIR analyses showed that the addition of Ca2+ hardly affected the adsorption of E.SA onto fluorapatite surface, which can be due to the formation and precipitation of calcium alginate. In contrast, NaOL remained weakly adsorbed on the fluorapatite surface even the presence of Ca2+ and E.SA. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Preoperative risk factors for conversion from laparoscopic to open cholecystectomy: a validated risk score derived from a prospective U.K. database of 8820 patients

Author

Fenwick, Stephen, Elmasry, Mohamed, Nunes, Quentin, Kennedy, David, Khan, Raja B., Khan, Muhammad A.S., Magee, Conor J., Jones, Steven M., Mason, Denise, Parappally, Ciny P., Mathur, Pawan, Saunders, Michael, Jamel, Sara, Haque, Samer U.l., Zafar, Sara, Shiwani, Muhammad H., Samuel, Nehemiah, Dar, Farooq, Jackson, Andrew, Lovett, Bryony, Dindyal, Shiva, Winter, Hannah, Rahman, Saquib, Wheatley, Kevin, Nieto, Tom, Ayaani, Soofiyah, Youssef, Haney, Nijjar, Rajwinder S., Watkin, Helen, Naumann, David, Emeshi, Sophie, Sarmah, Piyush B., Lee, Kathryn, Joji, Nikita, Heath, Jonathan, Teasdale, Rebecca L., Weerasinghe, Chamindri, Needham, Paul J., Welbourn, Hannah, Forster, Luke, Finch, David, Blazeby, Jane M., Robb, William, McNair, Angus G.K., Hrycaiczuk, Alex, Charalabopoulos, Alexandros, Kadirkamanathan, Sritharan, Tang, Cheuk-Bong, Jayanthi, Naga V.G., Noor, Nigel, Dobbins, Brian, Cockbain, Andrew J., Nilsen-Nunn, April, de Siqueira, Jonathan, Pellen, Mike, Cowley, Jonathan B., Ho, Wei-Min, Miu, Victor, White, Timothy J., Hodgkins, Kathryn A., Kinghorn, Alison, Tutton, Matthew G., Al-Abed, Yahya A., Menzies, Donald, Ahmad, Anwar, Reed, Joanna, Khan, Shabuddin, Monk, David, Vitone, Louis J., Murtaza, Ghulam, Joel, Abraham, Brennan, Stephen, Shier, David, Zhang, Catherine, Yoganathan, Thusidaran, Robinson, Steven J., McCallum, Iain J.D., Jones, Michael J., Elsayed, Mohammed, Tuck, Liz, Wayman, John, Carney, Kate, Aroori, Somaiah, Hosie, Kenneth B., Kimble, Adam, Bunting, David M., Fawole, Adeshina S., Basheer, Mohammed, Dave, Rajiv V., Sarveswaran, Janahan, Jones, Elinor, Kendal, Chris, Tilston, Michael P., Gough, Martin, Wallace, Tom, Singh, Shailendra, Downing, Justine, Mockford, Katherine A., Issa, Eyad, Shah, Nayab, Chauhan, Neal, Wilson, Timothy R., Forouzanfar, Amir, Wild, Jonathan R.L., Nofal, Emma, Bunnell, Catherine, Madbak, Khaliel, Rao, Sudhindra T.V., Devoto, Laurence, Siddiqi, Najaf, Khawaja, Zechan, Hewes, James C., Gould, Laura, Chambers, Alice, Rodriguez, Daniel U., Sen, Gourab, Robinson, Stuart, Bartlett, Francis, Rae, David M., Stevenson, Thomas E.J., Sarvananthan, Kas, Dwerryhouse, Simon J., Higgs, Simon M., Old, Oliver J., Hardy, Thomas J., Shah, Reena, Hornby, Steve T., Keogh, Ken, Frank, Lucinda, Al-Akash, Musallam, Upchurch, Emma A., Frame, Richard J., Hughes, Michael, Jelley, Clare, Weaver, Simon, Roy, Sudipta, Sillo, Toritseju O., Galanopoulos, Giorgios, Cuming, Tamzin, Cunha, Pedro, Tayeh, Salim, Kaptanis, Sarantos, Heshaishi, Mohamed, Eisawi, Abdalla, Abayomi, Michael, Ngu, Wee S., Fleming, Katie, Bajwa, Dalvir S., Chitre, Vivek, Aryal, Kamal, Ferris, Paul, Silva, Michael, Lammy, Simon, Mohamed, Sarah, Khawaja, Amir, Hussain, Adnan, Ghazanfar, Mudassar A., Bellini, Maria I., Ebdewi, Hamdi, Elshaer, Mohamed, Gravante, Gianpiero, Drake, Benjamin, Ogedegbe, Arikoge, Mukherjee, Dipankar, Arhi, Chanpreet, Giwa, Lola, Iqbal, Nusrat, Watson, Nicholas F., Aggarwal, Smeer K., Orchard, Philippa, Villatoro, Eduardo, Willson, Peter D., Mok, Kam W.J., Woodman, Thomas, Deguara, Jean, Garcea, Giuseppe, Babu, Benoy I., Dennison, Alistair R., Malde, Deep, Lloyd, David, Slavin, John P., Jones, Robert P., Ballance, Laura, Gerakopoulos, Stratos, Jambulingam, Periyathambi, Mansour, Sami, Sakai, Naomi, Acharya, Vikas, Sadat, Mohammed M., Karim, Lawen, Larkin, David, Amin, Khalid, Khan, Amarah, Law, Jennifer, Jamdar, Saurabh, Smith, Stella R., Sampat, Keerthika, O'shea, Kathryn M., Manu, Mangta, Asprou, Fotini M., Malik, Nabeela S., Chang, Jessica, Johnstone, Marianne, Lewis, Michael, Roberts, Geoffrey P., Karavadra, Babu, Photi, Evangelos, Hewes, James, Rodriguez, Dan, O'Reilly, Derek A., Rate, Anthony J., Sekhar, Hema, Henderson, Lucy T., Starmer, Benjamin Z., Coe, Peter O., Tolofari, Sotonye, Barrie, Jenifer, Bashir, Gareth, Sloane, Jake, Madanipour, Suroosh, Halkias, Constantine, Trevatt, Alexander E.J., Borowski, David W., Hornsby, Jane, Courtney, Michael J., Virupaksha, Suvi, Seymour, Keith, Robinson, Sarah, Hawkins, Helen, Bawa, Sadiq, Gallagher, Paul V., Reid, Alistair, Wood, Peter, Finch, Jonathan G., Finch, J.Guy, Parmar, Jitesh, Stirland, Euan, Gardner-Thorpe, James, Al-Muhktar, Ahmed, Peterson, Mark, Majeed, Ali, Bajwa, Farrukh M., Martin, Jack, Choy, Alfred, Tsang, Andrew, Pore, Naresh, Andrew, David R., Al-Khyatt, Waleed, Santosh Bhandari, Christopher Taylor, Chambers, Adam, Subramanium, Dhivya, Toh, Simon K.C., Carter, Nicholas C., Mercer, Stuart J., Knight, Benjamin, Vijay, Vardhini, Alagaratnam, Swethan, Sinha, Sidhartha, Khan, Shahab, El-Hasani, Shamsi S., Hussain, Abdulzahra A., Bhattacharya, Vish, Kansal, Nisheeth, Fasih, Tani, Jackson, Claire, Siddiqui, Midhat N., Chishti, Imran A., Fordham, Imogen J., Siddiqui, Zohaib, Bausbacher, Harald, Geogloma, Ileana, Gurung, Kabita, Tsavellas, George, Basynat, Pradeep, Shrestha, Ashish K., Basu, Sanjoy, Chhabra, Alok, Harilingam, Mohan, Rabie, Mohamed, Akhtar, Mansoor, Kumar, Pradeep, Jafferbhoy, Sadaf F., Hussain, Najam, Raza, Soulat, Haque, Manzarul, Alam, Imran, Aseem, Rabiya, Patel, Shakira, Asad, Mehek, Booth, Michael I., Ball, William R., Wood, Christopher P.J., Pinho-Gomes, Ana C., Kausar, Ambareen, Obeidallah, Mohammed, Varghase, Joseph, Lodhia, Joshil, Bradley, Donal, Rengifo, Carla, Lindsay, David, Gopalswamy, Sivakumar, Finlay, Ian, Wardle, Stacy, Bullen, Naomi, Iftikhar, Syed Y., Awan, Altaf, Ahmed, Javed, Leeder, Paul, Fusai, Guiseppe, Bond-Smith, Giles, Psica, Alicja, Puri, Yogesh, Hou, David, Noble, Fergus, Szentpali, Karoly, Broadhurst, Jack, Date, Ravindra, Hossack, Martin R., Goh, Yan L., Turner, Paul, Shetty, Vinutha, Riera, Manel, Macano, Christina A.W., Sukha, Anisha, Preston, Shaun R., Hoban, Jennifer R., Puntis, Daniel J., Williams, Sophie V., Krysztopik, Richard, Kynaston, James, Batt, Jeremy, Doe, Matthew, Goscimski, Andrzej, Jones, Gareth H., Hall, Claire, Carty, Nick, Ahmed, Jamil, Panteleimonitis, Sofoklis, Gunasekera, Rohan T., Sheel, Andrea R.G., Lennon, Hannah, Hindley, Caroline, Reddy, Marcus, Kenny, Ross, Elkheir, Natalie, McGlone, Emma R., Rajaganeshan, Rajasundaram, Hancorn, Kate, Hargreaves, Anita, Prasad, Raj, Longbotham, David A., Vijayanand, Dhakshinamoorthy, Wijetunga, Imeshi, Ziprin, Paul, Nicolay, Christopher R., Yeldham, Geoffrey, Read, Edward, Gossage, James A., Rolph, Rachel C., Ebied, Husam, Phull, Manraj, Khan, Mohammad A., Popplewell, Matthew, Kyriakidis, Dimitrios, Hussain, Anwar, Henley, Natasha, Packer, Jessica R., Derbyshire, Laura, Porter, Jonathan, Appleton, Shaun, Farouk, Marwan, Basra, Melvinder, Jennings, Neil A., Ali, Shahda, Kanakala, Venkatesh, Ali, Haythem, Lane, Risha, Dickson-Lowe, Richard, Zarsadias, Prizzi, Mirza, Darius, Puig, Sonia, Al Amari, Khalid, Vijayan, Deepak, Sutcliffe, Robert, Marudanayagam, Ravi, Hamady, Zayed, Prasad, Abheesh R., Patel, Abhilasha, Durkin, Damien, Kaur, Parminder, Bowen, Laura, Byrne, James P., Pearson, Katherine L., Delisle, Theo G., Davies, James, Tomlinson, Mark A., Johnpulle, Michelle A., Slawinski, Corinna, Macdonald, Andrew, Nicholson, James, Newton, Katy, Mbuvi, James, Farooq, Ansar, Mothe, Bhavani S., Zafrani, Zakhi, Brett, Daniel, Francombe, James, Spreadborough, Philip, Barnes, James, Cheung, Melanie, Al-Bahrani, Ahmed Z., Preziosi, Giuseppe, Urbonas, Tomas, Alberts, Justin, Mallik, Mekhlola, Patel, Krashna, Segaran, Ashvina, Doulias, Triantafyllos, Sufi, Pratik A., Yao, Caroline, Pollock, Sarah, Manzelli, Antonio, Wajed, Saj, Kourkulos, Michail, Pezzuto, Roberto, Wadley, Martin, Hamilton, Emma, Jaunoo, Shameen, Padwick, Robert, Sayegh, Mazin, Newton, Richard C., Hebbar, Madhusoodhana, Farag, Sameh F., Hebbar, Madhu, Spearman, John, Hamdan, Mohammed F., D'Costa, Conrad, Blane, Christine, Giles, Mathew, Peter, Mark B., Hirst, Natalie A., Hossain, Tanvir, Pannu, Arslan, El-Dhuwaib, Yesar, Morrison, Tamsin E.M., Taylor, Greg W., Thompson, Ronald L.E., McCune, Ken, Loughlin, Paula, Lawther, Roger, Byrnes, Colman K., Simpson, Duncan J., Mawhinney, Abi, Warren, Conor, McKay, Damian, McIlmunn, Colin, Martin, Serena, MacArtney, Matthew, Diamond, Tom, Davey, Phil, Jones, Claire, Clements, Joshua M., Digney, Ruairi, Chan, Wei M., McCain, Stephen, Gull, Sadaf, Janeczko, Adam, Dorrian, Emmet, Harris, Andrew, Dawson, Suzanne, Johnston, Dorothy, McAree, Barry, Ghareeb, Essam, Thomas, George, Connelly, Martin, McKenzie, Stephen, Cieplucha, Krzysztos, Spence, Gary, Campbell, William, Hooks, Gareth, Bradley, Neil, Hill, Arnold D.K., Cassidy, John T., Boland, Michael, Burke, Paul, Nally, Deirdre M., Khogali, Elmoataz, Shabo, Wael, Iskandar, Edrin, McEntee, Gerry P., O'Neill, Maeve A., Peirce, Colin, Lyons, Emma M., O'Sullivan, Adrian W., Thakkar, Rohan, Carroll, Paul, Ivanovski, Ivan, Balfe, Paul, Lee, Matthew, Winter, Des C., Kelly, Michael E., Hoti, Emir, Maguire, Donal, Karunakaran, Priyadarssini, Geoghegan, Justin G., Martin, Sean T., Cross, Keith S., Cooke, Fiachra, Zeeshan, Saquib, Murphy, James O., Mealy, Ken, Mohan, Helen M., Nedujchelyn, Yuwaraja, Ullah, Muhammad F., Ahmed, Irfan, Giovinazzo, Francesco, Milburn, James, Prince, Sarah, Brooke, Eleanor, Buchan, Joanna, Khalil, Ahmed M., Vaughan, Elizabeth M., Ramage, Michael I., Aldridge, Roland C., Gibson, Simon, Nicholson, Gary A., Vass, David G., Grant, Alan J., Holroyd, David J., Jones, Angharad, Sutton, Cherith M.L.R., O'Dwyer, Patrick, Nilsson, Frida, Weber, Beatrix, Williamson, Tracey K., Lalla, Kushik, Bryant, Alice, Carter, Ross, Forrest, Craig R., Hunter, David I., Nassar, Ahmad H., Orizu, Mavis N., Knight, Katrina, Qandeel, Haitham, Suttie, Stuart, Belding, Rowena, McClarey, Andrew, Boyd, Alan T., Guthrie, Graeme J.K., Lim, Pei J., Luhmann, Andreas, Watson, Angus J.M., Richards, Colin H., Nicol, Laura, Madurska, Marta, Harrison, Ewen, Boyce, Kathryn M., Roebuck, Amanda, Ferguson, Graeme, Pati, Pradeep, Wilson, Michael S.J., Dalgaty, Faith, Fothergill, Laura, Driscoll, Peter J., Mozolowski, Kirsty L., Banwell, Victoria, Bennett, Stephen P., Rogers, Paul N., Skelly, Brendan L., Rutherford, Claire L., Mirza, Ahmed K., Lazim, Taha, Lim, Henry C.C., Duke, Diana, Ahmed, Talat, Beasley, William D., Wilkinson, Marc D., Maharaj, Geta, Malcolm, Cathy, Brown, Timothy H., Shingler, Guy M., Mowbray, Nicholas, Radwan, Rami, Morcous, Paul, Wood, Simon, Kadhim, Abbas, Stewart, Duncan J., Baker, Andrew L., Tanner, Nicola, Shenoy, Hrishikesh, Sutcliffe, Robert P., Hollyman, Marianne, Hodson, James, Bonney, Glenn, Vohra, Ravi S., and Griffiths, Ewen A.

Published
2016
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21. Turnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes

Author

Agence Nationale de la Recherche (France), Swiss National Science Foundation, Région Grand Est (France), Ministerio de Economía y Competitividad (España), German Research Foundation, Pitzalis, Nicolas [0000-0003-0453-7393], Amari, Khalid [0000-0001-6594-6938], Pflieger, David [0000-0002-4332-1379], Donaire, Livia [0000-0002-5454-2994], Wassenegger, Michael [0000-0002-7926-2454], Llave, César [0000-0003-3844-4582], Heinlein, Manfred [0000-0001-7322-1654], Pitzalis, Nicolas, Amari, Khalid, Graindorge, Stéfanie, Pflieger, David, Donaire, Livia, Wassenegger, Michael, Llave, César, Heinlein, Manfred, Agence Nationale de la Recherche (France), Swiss National Science Foundation, Région Grand Est (France), Ministerio de Economía y Competitividad (España), German Research Foundation, Pitzalis, Nicolas [0000-0003-0453-7393], Amari, Khalid [0000-0001-6594-6938], Pflieger, David [0000-0002-4332-1379], Donaire, Livia [0000-0002-5454-2994], Wassenegger, Michael [0000-0002-7926-2454], Llave, César [0000-0003-3844-4582], Heinlein, Manfred [0000-0001-7322-1654], Pitzalis, Nicolas, Amari, Khalid, Graindorge, Stéfanie, Pflieger, David, Donaire, Livia, Wassenegger, Michael, Llave, César, and Heinlein, Manfred

Abstract

Virus-induced plant diseases in cultivated plants cause important damages in yield. Although the mechanisms of virus infection are intensely studied at the cell biology level, only little is known about the molecular dialog between the invading virus and the host genome. Here we describe a combinatorial genome-wide approach to identify networks of sRNAs-guided posttranscriptional regulation within local Turnip mosaic virus (TuMV) infection sites in Brassica napus leaves. We show that the induction of host-encoded, virus-activated small interfering RNAs (vasiRNAs) observed in virus-infected tissues is accompanied by site-specific cleavage events on both viral and host RNAs that recalls the activity of small RNA-induced silencing complexes (RISC). Cleavage events also involve virus-derived siRNA (vsiRNA)–directed cleavage of target host transcripts as well as cleavage of viral RNA by both host vasiRNAs and vsiRNAs. Furthermore, certain coding genes act as virus-activated regulatory hubs to produce vasiRNAs for the targeting of other host genes. The observations draw an advanced model of plant-virus interactions and provide insights into the complex regulatory networking at the plant-virus interface within cells undergoing early stages of infection.

Published
2020

30. Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy

Author

Griffiths, Ewen A, Hodson, James, Vohra, Ravi S, Marriott, Paul, Katbeh, Tarek, Zino, Samer, Nassar, Ahmad HM, Vohra, Ravinder S, Kirkham, Amanda J, Pasquali, Sandro, Johnstone, Marianne, Spreadborough, Philip, Alderson, Derek, Fenwick, Stephen, Elmasry, Mohamed, Nunes, Quentin M, Kennedy, David, Khan, Raja Basit, Khan, Muhammad AS, Magee, Conor J, Jones, Steven M, Mason, Denise, Parappally, Ciny P, Mathur, Pawan, Saunders, Michael, Jamel, Sara, Haque, Samer Ul, Zafar, Sara, Shiwani, Muhammad Hanif, Samuel, Nehemiah, Dar, Farooq, Jackson, Andrew, Lovett, Bryony, Dindyal, Shiva, Winter, Hannah, Fletcher, Ted, Rahman, Saquib, Wheatley, Kevin, Nieto, Tom, Ayaani, Soofiyah, Youssef, Haney, Nijjar, Rajwinder S, Watkin, Helen, Naumann, David, Emesih, Sophie, Sarmah, Piyush B, Lee, Kathryn, Joji, Nikita, Lambert, Joel, Heath, Jonathan, Teasdale, Rebecca L, Weerasinghe, Chamindri, Needham, Paul J, Welbourn, Hannah, Forster, Luke, Finch, David, Blazeby, Jane M, Robb, William, McNair, Angus GK, Hrycaiczuk, Alex, Charalabopoulos, Alexandros, Kadirkamanathan, Sritharan, Tang, Cheuk-Bong, Jayanthi, Naga VG, Noor, Nigel, Dobbins, Brian, Cockbain, Andrew J, Nilsen-Nunn, April, de Siqueira, Jonathan, Pellen, Mike, Cowley, Jonathan B, Ho, Wei-Min, Miu, Victor, White, Timothy J, Hodgkins, Kathryn A, Kinghorn, Alison, Tutton, Matthew G, Al-Abed, Yahya A, Menzies, Donald, Ahmad, Anwar, Reed, Joanna, Khan, Shabuddin, Monk, David, Vitone, Louis J, Murtaza, Ghulam, Joel, Abraham, Brennan, Stephen, Shier, David, Zhang, Catherine, Yoganathan, Thusidaran, Robinson, Steven J, McCallum, Iain JD, Jones, Michael J, Elsayed, Mohammed, Tuck, Liz, Wayman, John, Carney, Kate, Aroori, Somaiah, Hosie, Kenneth B, Kimble, Adam, Bunting, David M, Fawole, Adeshina S, Basheer, Mohammed, Dave, Rajiv V, Sarveswaran, Janahan, Jones, Elinor, Kendal, Chris, Tilston, Michael P, Gough, Martin, Wallace, Tom, Singh, Shailendra, Downing, Justine, Mockford, Katherine A, Issa, Eyad, Shah, Nayab, Chauhan, Neal, Wilson, Timothy R, Forouzanfar, Amir, Wild, Jonathan RL, Nofal, Emma, Bunnell, Catherine, Madbak, Khaliel, Rao, Sudhindra TV, Devoto, Laurence, Siddiqi, Najaf, Khawaja, Zechan, Hewes, James C, Gould, Laura, Chambers, Alice, Rodriguez, Daniel Urriza, Sen, Gourab, Robinson, Stuart, Bartlett, Francis, Rae, David M, Stevenson, Thomas EJ, Sarvananthan, Kas, Dwerryhouse, Simon J, Higgs, Simon M, Old, Oliver J, Hardy, Thomas J, Shah, Reena, Hornby, Steve T, Keogh, Ken, Frank, Lucinda, Al-Akash, Musallam, Upchurch, Emma A, Frame, Richard J, Hughes, Michael, Jelley, Clare, Weaver, Simon, Roy, Sudipta, Sillo, Toritseju O, Galanopoulos, Giorgios, Cuming, Tamzin, Cunha, Pedro, Tayeh, Salim, Kaptanis, Sarantos, Heshaishi, Mohamed, Eisawi, Abdalla, Abayomi, Michael, Ngu, Wee Sing, Fleming, Katie, Bajwa, Dalvi S, Chitre, Vivek, Aryal, Kamal, Ferris, Paul, Silva, Michael, Lammy, Simon, Mohamed, Sarah, Khawaja, Amir, Hussain, Adnan, Ghazanfar, Mudassar A, Bellini, Maria Irene, Ebdewi, Hamdi, Elshaer, Mohamed, Gravante, Gianpiero, Drake, Benjamin, Ogedegbe, Arikoge, Mukherjee, Dipankar, Arhi, Chanpreet, Giwa, Lola, Iqbal, Nusrat, Watson, Nicholas F, Aggarwal, Smeer Kumar, Orchard, Philippa, Villatoro, Eduardo, Willson, Peter D, Mok, Kam Wa Jessica, Woodman, Thomas, Deguara, Jean, Garcea, Giuseppe, Babu, Benoy I, Dennison, AR, Malde, Deep, Lloyd, David, Satheesan, Steve, Al-Taan, Omer, Boddy, Alexander, Slavin, John P, Jones, Robert P, Ballance, Laura, Gerakopoulos, Stratos, Jambulingam, Periyathambi, Mansour, Sami, Sakai, Naomi, Acharya, Vikas, Sadat, Mohammed M, Karim, Lawen, Larkin, David, Amin, Khalid, Khan, Amarah, Law, Jennifer, Jamdar, Saurabh, Smith, Stella R, Sampat, Keerthika, O'shea, Kathryn M, Manu, Mangta, Asprou, Fotini M, Malik, Nabeela S, Chang, Jessica, Lewis, Michael, Roberts, Geoffrey P, Karavadra, Babu, Photi, Evangelos, Hewes, James, Rodriguez, Dan, O'Reilly, Derek A, Rate, Anthony J, Sekhar, Hema, Henderson, Lucy T, Starmer, Benjamin Z, Coe, Peter O, Tolofari, Sotonye, Barrie, Jenifer, Bashir, Gareth, Sloane, Jake, Madanipour, Suroosh, Halkias, Constantine, Trevatt, Alexander EJ, Borowski, David W, Hornsby, Jane, Courtney, Michael J, Virupaksha, Suvi, Seymour, Keith, Robinson, Sarah, Hawkins, Helen, Bawa, Sadiq, Gallagher, Paul V, Reid, Alistair, Wood, Peter, Finch, JG, Finch, J Guy, Parmar, J, Stirland, E, Gardner-Thorpe, James, Al-Muhktar, Ahmed, Peterson, Mark, Majeed, Ali, Bajwa, Farrukh M, Martin, Jack, Choy, Alfred, Tsang, Andrew, Pore, Naresh, Andrew, David R, Al-Khyatt, Waleed, Bhandari, Christopher Taylor Santosh, Chambers, Adam, Subramanium, Dhivya, Toh, Simon KC, Carter, Nicholas C, Tate, Sophie, Pearce, Belinda, Wainwright, Denise, Mercer, Stuart J, Knight, Benjamin, Vijay, Vardhini, Alagaratnam, Swethan, Sinha, Sidhartha, Khan, Shahab, El-Hasani, Shamsi S, Hussain, Abdulzahra A, Bhattacharya, Vish, Kansal, Nisheeth, Fasih, Tani, Jackson, Claire, Siddiqui, Midhat N, Chishti, Imran A, Fordham, Imogen J, Siddiqui, Zohaib, Bausbacher, Harald, Geogloma, Ileana, Gurung, Kabita, Tsavellas, George, Basynat, Pradeep, Shrestha, Ashish Kiran, Basu, Sanjoy, Harilingam, Alok Chhabra Mohan, Rabie, Mohamed, Akhtar, Mansoor, Kumar, Pradeep, Jafferbhoy, Sadaf F, Hussain, Najam, Raza, Soulat, Haque, Manzarul, Alam, Imran, Aseem, Rabiya, Patel, Shakira, Asad, Mehek, Booth, Michael I, Ball, William R, Wood, Christopher PJ, Pinho-Gomes, Ana C, Kausar, Ambareen, Obeidallah, Moh'd Rami, Varghase, Joseph, Lodhia, Joshil, Bradley, Donal, Rengifo, Carla, Lindsay, David, Gopalswamy, Sivakumar, Finlay, Ian, Wardle, Stacy, Bullen, Naomi, Iftikhar, Syed Yusuf, Awan, Altaf, Ahmed, Javed, Leeder, Paul, Fusai, Guiseppe, Bond-Smith, Giles, Psica, Alicja, Puri, Yogesh, Hou, David, Noble, Fergus, Szentpali, Karoly, Broadhurst, Jack, Date, Ravindra, Hossack, Martin R, Goh, Yan Li, Turner, Paul, Shetty, Vinutha, Riera, Manel, Macano, Christina AW, Sukha, Anisha, Preston, Shaun R, Hoban, Jennifer R, Puntis, Daniel J, Williams, Sophie V, Krysztopik, Richard, Kynaston, James, Batt, Jeremy, Doe, Matthew, Goscimski, Andrzej, Jones, Gareth H, Hall, Claire, Carty, Nick, Ahmed, Jamil, Panteleimonitis, Sofoklis, Gunasekera, Rohan T, Sheel, Andrea RG, Lennon, Hannah, Hindley, Caroline, Reddy, Marcus, Kenny, Ross, Elkheir, Natalie, McGlone, Emma R, Rajaganeshan, Rajasundaram, Hancorn, Kate, Hargreaves, Anita, Prasad, Raj, Longbotham, David A, Vijayanand, Dhakshinamoorthy, Wijetunga, Imeshi, Ziprin, Paul, Nicolay, Christopher R, Yeldham, Geoffrey, Read, Edward, Gossage, James A, Rolph, Rachel C, Ebied, Husam, Phull, Manraj, Khan, Mohammad A, Popplewell, Matthew, Kyriakidis, Dimitrios, Hussain, Anwar, Henley, Natasha, Packer, Jessica R, Derbyshire, Laura, Porter, Jonathan, Appleton, Shaun, Farouk, Marwan, Basra, Melvinder, Jennings, Neil A, Ali, Shahda, Kanakala, Venkatesh, Ali, Haythem, Lane, Risha, Dickson-Lowe, Richard, Zarsadias, Prizzi, Mirza, Darius, Puig, Sonia, Al Amari, Khalid, Vijayan, Deepak, Sutcliffe, Robert, Marudanayagam, Ravi, Hamady, Zayed, Prasad, Abheesh R, Patel, Abhilasha, Durkin, Damien, Kaur, Parminder, Bowen, Laura, Byrne, James P, Pearson, Katherine L, Delisle, Theo G, Davies, James, Tomlinson, Mark A, Johnpulle, Michelle A, Slawinski, Corinna, Macdonald, Andrew, Nicholson, James, Newton, Katy, Mbuvi, James, Farooq, Ansar, Mothe, Bhavani Sidhartha, Zafrani, Zakhi, Brett, Daniel, Francombe, James, Barnes, James, Cheung, Melanie, Al-Bahrani, Ahmed Z, Preziosi, Giuseppe, Urbonas, Tomas, Alberts, Justin, Mallik, Mekhlola, Patel, Krashna, Segaran, Ashvina, Doulias, Triantafyllos, Sufi, Pratik A, Yao, Caroline, Pollock, Sarah, Manzelli, Antonio, Wajed, Saj, Kourkulos, Michail, Pezzuto, Roberto, Wadley, Martin, Hamilton, Emma, Jaunoo, Shameen, Padwick, Robert, Sayegh, Mazin, Newton, Richard C, Hebbar, Madhusoodhana, Farag, Sameh F, Spearman, John, Hamdan, Mohammed F, D'Costa, Conrad, Blane, Christine, Giles, Mathew, Peter, Mark B, Hirst, Natalie A, Hossain, Tanvir, El-Dhuwaib, Arslan Pannu Yesar, Morrison, Tamsin EM, Taylor, Greg W, Thompson, Ronald LE, McCune, Ken, Loughlin, Paula, Lawther, Roger, Byrnes, Colman K, Simpson, Duncan J, Mawhinney, Abi, Warren, Conor, McKay, Damian, McIlmunn, Colin, Martin, Serena, MacArtney, Matthew, Diamond, Tom, Davey, Phil, Jones, Claire, Clements, Joshua M, Digney, Ruairi, Chan, Wei Ming, McCain, Stephen, Gull, Sadaf, Janeczko, Adam, Dorrian, Emmet, Harris, Andrew, Dawson, Suzanne, Johnston, Dorothy, McAree, Barry, Ghareeb, Essam, Thomas, George, Connelly, Martin, McKenzie, Stephen, Cieplucha, Krzysztos, Spence, Gary, Campbell, William, Hooks, Gareth, Bradley, Neil, Hill, Arnold DK, Cassidy, John T, Boland, Michael, Burke, Paul, Nally, Deirdre M, Khogali, Elmoataz, Shabo, Wael, Iskandar, Edrin, McEntee, Gerry P, O'Neill, Maeve A, Peirce, Colin, Lyons, Emma M, O'Sullivan, Adrian W, Thakkar, Rohan, Carroll, Paul, Ivanovski, Ivan, Balfe, Paul, Lee, Matthew, Winter, Des C, Kelly, Michael E, Hoti, Emir, Maguire, Donal, Karunakaran, Priyadarssini, Geoghegan, Justin G, McDermott, Frank, Martin, Sean T, Cross, Keith S, Cooke, Fiachra, Zeeshan, Saquib, Murphy, James O, Mealy, Ken, Mohan, Helen M, Nedujchelyn, Yuwaraja, Ullah, Muhammad Fahad, Ahmed, Irfan, Giovinazzo, Francesco, Milburn, James, Prince, Sarah, Brooke, Eleanor, Buchan, Joanna, Khalil, Ahmed M, Vaughan, Elizabeth M, Ramage, Michael I, Aldridge, Roland C, Gibson, Simon, Nicholson, Gary A, Vass, David G, Grant, Alan J, Holroyd, David J, Jones, M Angharad, Sutton, Cherith MLR, O'Dwyer, Patrick, Nilsson, Frida, Weber, Beatrix, Williamson, Tracey K, Lalla, Kushik, Bryant, Alice, Carter, C Ross, Forrest, Craig R, Hunter, David I, Nassar, Ahmad H, Orizu, Mavis N, Knight, Katrina, Qandeel, Haitham, Suttie, Stuart, Belding, Rowena, McClarey, Andrew, Boyd, Alan T, Guthrie, Graeme JK, Lim, Pei J, Luhmann, Andreas, Watson, Angus JM, Richards, Colin H, Nicol, Laura, Madurska, Marta, Harrison, Ewen, Boyce, Kathryn M, Roebuck, Amanda, Ferguson, Graeme, Pati, Pradeep, Wilson, Michael SJ, Dalgaty, Faith, Fothergill, Laura, Driscoll, Peter J, Mozolowski, Kirsty L, Banwell, Victoria, Bennett, Stephen P, Rogers, Paul N, Skelly, Brendan L, Rutherford, Claire L, Mirza, Ahmed K, Lazim, Taha, Lim, Henry CC, Duke, Diana, Ahmed, Talat, Beasley, William D, Wilkinson, Marc D, Maharaj, Geta, Malcolm, Cathy, Brown, Timothy H, Al-Sarireh, Bilal, Shingler, Guy M, Mowbray, Nicholas, Radwan, Rami, Morcous, Paul, Wood, Simon, Kadhim, Abbas, Stewart, Duncan J, Baker, Andrew L, Tanner, Nicola, Shenoy, Hrishikesh, Hafiz, Shazia, De Marchi, Joshua A, Singh-Ranger, Deepak, Hisham, Elzanati, Ainley, Paul, O'Neill, Stephen, Terrace, John, Napetti, Sara, Hopwood, Benjamin, Rhys, Thomas, Kanavati, Osama, Coats, Maria, Aleksandrov, Danail, Kallaway, Charlotte, Yahya, Salama, Templeton, Alexa, Trotter, Martin, Lo, Christina, Dhillon, Ajit, Heywood, Nick, Aawsaj, Yousif, Hamdan, Alhafidz, Reece-Bolton, Obuobi, McGuigan, Andrew, Shahin, Yousef, Aymon, Luther, Ali Alison, Nicholson, James A, Rajendran, Ilayaraja, Boal, Matthew, Ritchie, Judith, Grp, CholeS Study, Collaborative, West Midlands Res, Segaran, A, and Collaborative, West Midlands Research

Subjects
medicine.medical_specialty, medicine.medical_treatment, 030230 surgery, Article, 03 medical and health sciences, 0302 clinical medicine, Case mix index, Laparoscopic, length of stay, male, middle aged, postoperative complications, Medicine, Cholecystectomy, Prospective cohort study, Grading (education), humans, Laparoscopic cholecystectomy, business.industry, General surgery, adult, cholecystectomy, difficulty grading, laparoscopic, operative difficulty, surgery, aged, cholecystectomy, laparoscopic, conversion to open surgery, female, multivariate analysis, prospective studies, ROC curve, Difficulty grading, Cohort, 030211 gastroenterology & hepatology, Surgery, business, Operative difficulty, Grading scale, Abdominal surgery
Abstract

Background A reliable system for grading operative difficulty of laparoscopic cholecystectomy would standardise description of findings and reporting of outcomes. The aim of this study was to validate a difficulty grading system (Nassar scale), testing its applicability and consistency in two large prospective datasets. Methods Patient and disease-related variables and 30-day outcomes were identified in two prospective cholecystectomy databases: the multi-centre prospective cohort of 8820 patients from the recent CholeS Study and the single-surgeon series containing 4089 patients. Operative data and patient outcomes were correlated with Nassar operative difficultly scale, using Kendall’s tau for dichotomous variables, or Jonckheere–Terpstra tests for continuous variables. A ROC curve analysis was performed, to quantify the predictive accuracy of the scale for each outcome, with continuous outcomes dichotomised, prior to analysis. Results A higher operative difficulty grade was consistently associated with worse outcomes for the patients in both the reference and CholeS cohorts. The median length of stay increased from 0 to 4 days, and the 30-day complication rate from 7.6 to 24.4% as the difficulty grade increased from 1 to 4/5 (both p p Conclusion We have shown that an operative difficulty scale can standardise the description of operative findings by multiple grades of surgeons to facilitate audit, training assessment and research. It provides a tool for reporting operative findings, disease severity and technical difficulty and can be utilised in future research to reliably compare outcomes according to case mix and intra-operative difficulty.

Published
2018

32. On-farm Untersuchungen zum Nanovirus PNYDV (Pea necrotic yellow dwarf virus) an Ackerbohne (Vicia faba)

Author

Seeger, Judith Nora, Ziebell, Heiko, Amari, Khalid, Then, Christiane, Möckel, Thomas, Grüner, Esther, Sturm, Barbara, Nasirahmadi, Abozar, Shrestha, Luna, Böhm, Herwart, Saucke, Helmut, Mühlrath, Daniel, Albrecht, Joana, Finckh, Maria R., Hamm, Ulrich, Heß, Jürgen, Knierim, Ute, and Möller, Detlev

Subjects
Crop health, quality, protection, Cereals, pulses and oilseeds
Abstract

Pea necrotic yellow dwarf virus (PNYDV) ist ein neuer Nanovirus in Mitteleurope, der verschiedene Leguminosen befällt und persistent durch Blattläuse übertragen wird. In dieser on-farm Studie untersuchten wir sechs Ackerbohnen-Felder mit PNYDV-symptomatischen Nestern mittels multispektraler Drohnen-Aufnahmen, die mit ground-truth Pflanzenwachstumsparametern korreliert werden. Symptomatische Pflanzen zeigten einen signifikanten Rückgang der Sprosslänge, oberirdische Trockenmasse, Hülsenzahl sowie Anzahl und Trockenmasse N-fixierender Wurzelknöllchen. Im Gegensatz zu gesunden Pflanzen hatten symptomatische Pflanzen keine rosa oder rot gefärbten Knöllcheninhalte, stattdessen höhere Anteile zerfallender und inaktiver Knöllchen. Verschiedene Indices der multispektralen Aufnahmen zeigten einen deutlichen Unterschied in der spekteralen Information von symptomatischem Infektionsherd gegenüber der umgebenden Referenz.

Published
2019

33. On-farm Untersuchungen zum Nanovirus PNYDV (Pea necrotic yellow dwarf virus) an Ackerbohne (Vicia faba)

Author

Mühlrath, Daniel, Albrecht, Joana, Finckh, Maria R., Hamm, Ulrich, Heß, Jürgen, Knierim, Ute, Möller, Detlev, Seeger, Judith Nora, Ziebell, Heiko, Amari, Khalid, Then, Christiane, Möckel, Thomas, Grüner, Esther, Sturm, Barbara, Nasirahmadi, Abozar, Shrestha, Luna, Böhm, Herwart, Saucke, Helmut, Mühlrath, Daniel, Albrecht, Joana, Finckh, Maria R., Hamm, Ulrich, Heß, Jürgen, Knierim, Ute, Möller, Detlev, Seeger, Judith Nora, Ziebell, Heiko, Amari, Khalid, Then, Christiane, Möckel, Thomas, Grüner, Esther, Sturm, Barbara, Nasirahmadi, Abozar, Shrestha, Luna, Böhm, Herwart, and Saucke, Helmut

Abstract

Pea necrotic yellow dwarf virus (PNYDV) ist ein neuer Nanovirus in Mitteleurope, der verschiedene Leguminosen befällt und persistent durch Blattläuse übertragen wird. In dieser on-farm Studie untersuchten wir sechs Ackerbohnen-Felder mit PNYDV-symptomatischen Nestern mittels multispektraler Drohnen-Aufnahmen, die mit ground-truth Pflanzenwachstumsparametern korreliert werden. Symptomatische Pflanzen zeigten einen signifikanten Rückgang der Sprosslänge, oberirdische Trockenmasse, Hülsenzahl sowie Anzahl und Trockenmasse N-fixierender Wurzelknöllchen. Im Gegensatz zu gesunden Pflanzen hatten symptomatische Pflanzen keine rosa oder rot gefärbten Knöllcheninhalte, stattdessen höhere Anteile zerfallender und inaktiver Knöllchen. Verschiedene Indices der multispektralen Aufnahmen zeigten einen deutlichen Unterschied in der spekteralen Information von symptomatischem Infektionsherd gegenüber der umgebenden Referenz.

Published
2019

34. Correction to: Utilisation of an operative difficulty grading scale for laparoscopic cholecystectomy (Surgical Endoscopy, (2018), 10.1007/s00464-018-6281-2)

Author

Griffiths, Ewen A., Hodson, James, Vohra, Ravi S., Marriott, Paul, Katbeh, Tarek, Zino, Samer, Nassar, Ahmad H. M., Vohra, Ravinder S., Kirkham, Amanda J., Pasquali, Sandro, Johnstone, Marianne, Spreadborough, Philip, Alderson, Derek, Fenwick, Stephen, Elmasry, Mohamed, Nunes, Quentin M., Kennedy, David, Khan, Raja Basit, Khan, Muhammad A. S., Magee, Conor J., Jones, Steven M., Mason, Denise, Parappally, Ciny P., Mathur, Pawan, Saunders, Michael, Jamel, Sara, Haque, Samer Ul, Zafar, Sara, Shiwani, Muhammad Hanif, Samuel, Nehemiah, Dar, Farooq, Jackson, Andrew, Lovett, Bryony, Dindyal, Shiva, Winter, Hannah, Fletcher, Ted, Rahman, Saquib, Wheatley, Kevin, Nieto, Tom, Ayaani, Soofiyah, Youssef, Haney, Nijjar, Rajwinder S., Watkin, Helen, Naumann, David, Emesih, Sophie, Sarmah, Piyush B., Lee, Kathryn, Joji, Nikita, Lambert, Joel, Heath, Jonathan, Teasdale, Rebecca L., Weerasinghe, Chamindri, Needham, Paul J., Welbourn, Hannah, Forster, Luke, Finch, David, Blazeby, Jane M., Robb, William, Mcnair, Angus G. K., Hrycaiczuk, Alex, Charalabopoulos, Alexandros, Kadirkamanathan, Sritharan, Tang, Cheuk-Bong, Jayanthi, Naga V. G., Noor, Nigel, Dobbins, Brian, Cockbain, Andrew J., Nilsen-Nunn, April, de Siqueira, Jonathan, Pellen, Mike, Cowley, Jonathan B., Wei-Min, Ho, Miu, Victor, White, Timothy J., Hodgkins, Kathryn A., Kinghorn, Alison, Tutton, Matthew G., Al-Abed, Yahya A., Menzies, Donald, Ahmad, Anwar, Reed, Joanna, Khan, Shabuddin, Monk, David, Vitone, Louis J., Murtaza, Ghulam, Joel, Abraham, Brennan, Stephen, Shier, David, Zhang, Catherine, Yoganathan, Thusidaran, Robinson, Steven J., Mccallum, Iain J. D., Jones, Michael J., Elsayed, Mohammed, Tuck, Liz, Wayman, John, Carney, Kate, Aroori, Somaiah, Hosie, Kenneth B., Kimble, Adam, Bunting, David M., Fawole, Adeshina S., Basheer, Mohammed, Dave, Rajiv V., Sarveswaran, Janahan, Jones, Elinor, Kendal, Chris, Tilston, Michael P., Gough, Martin, Wallace, Tom, Singh, Shailendra, Mockford, Justine Downing Katherine A., Issa, Eyad, Shah, Nayab, Chauhan, Neal, Wilson, Timothy R., Forouzanfar, Amir, Wild, Jonathan R. L., Nofal, Emma, Bunnell, Catherine, Madbak, Khaliel, Rao, Sudhindra T. V., Devoto, Laurence, Siddiqi, Najaf, Khawaja, Zechan, Hewes, James C., Gould, Laura, Chambers, Alice, Rodriguez, Daniel Urriza, Sen, Gourab, Robinson, Stuart, Bartlett, Francis, Rae, David M., Stevenson, Thomas E. J., Sarvananthan, Kas, Dwerryhouse, Simon J., Higgs, Simon M., Old, Oliver J., Hardy, Thomas J., Hornby, Reena Shah Steve T., Keogh, Ken, Frank, Lucinda, Al-Akash, Musallam, Upchurch, Emma A., Frame, Richard J., Hughes, Michael, Jelley, Clare, Weaver, Simon, Roy, Sudipta, Sillo, Toritseju O., Galanopoulos, Giorgios, Cuming, Tamzin, Cunha, Pedro, Tayeh, Salim, Kaptanis, Sarantos, Heshaishi, Mohamed, Eisawi, Abdalla, Abayomi, Michael, Ngu, Wee Sing, Fleming, Katie, Bajwa, Dalvir S., Chitre, Vivek, Aryal, Kamal, Ferris, Paul, Silva, Michael, Mohamed, Simon Lammy Sarah, Khawaja, Amir, Hussain, Adnan, Ghazanfar, Mudassar A., Bellini, Maria Irene, Ebdewi, Hamdi, Elshaer, Mohamed, Gravante, Gianpiero, Drake, Benjamin, Ogedegbe, Arikoge, Mukherjee, Dipankar, Arhi, Chanpreet, Iqbal, Lola Giwa Nusrat, Watson, Nicholas F., Aggarwal, Smeer Kumar, Orchard, Philippa, Villatoro, Eduardo, Willson, Peter D., Mok, Kam Wa Jessica, Woodman, Thomas, Deguara, Jean, Garcea, Giuseppe, Babu, Benoy I., Dennison, A. R., Malde, Deep, Lloyd, David, Satheesan, Steve, Al-Taan, Omer, Boddy, Alexander, Slavin, John P., Jones, Robert P., Ballance, Laura, Gerakopoulos, Stratos, Jambulingam, Periyathambi, Mansour, Sami, Sakai, Naomi, Acharya, Vikas, Sadat, Mohammed M., Karim, Lawen, Larkin, David, Amin, Khalid, Khan, Amarah, Law, Jennifer, Jamdar, Saurabh, Smith, Stella R., Sampat, Keerthika, O’Shea, Kathryn M., Manu, Mangta, Asprou, Fotini M., Malik, Nabeela S., Chang, Jessica, Lewis, Michael, Roberts, Geoffrey P., Karavadra, Babu, Photi, Evangelos, Hewes, James, Rodriguez, Dan, O’Reilly, Derek A., Rate, Anthony J., Sekhar, Hema, Henderson, Lucy T., Starmer, Benjamin Z., Coe, Peter O., Tolofari, Sotonye, Barrie, Jenifer, Bashir, Gareth, Sloane, Jake, Madanipour, Suroosh, Halkias, Constantine, Trevatt, Alexander E. J., Borowski, David W., Hornsby, Jane, Courtney, Michael J., Virupaksha, Suvi, Seymour, Keith, Robinson, Sarah, Hawkins, Helen, Bawa, Sadiq, Gallagher, Paul V., Reid, Alistair, Wood, Peter, Finch, J. G., Guy Finch, J., Parmar, J., Stirland, E., Gardner-Thorpe, James, Al-Muhktar, Ahmed, Peterson, Mark, Majeed, Ali, Bajwa, Farrukh M., Martin, Jack, Choy, Alfred, Tsang, Andrew, Pore, Naresh, Andrew, David R., Al-Khyatt, Waleed, Bhandari, Christopher Taylor Santosh, Chambers, Adam, Subramanium, Dhivya, Toh, Simon K. C., Carter, Nicholas C., Tate, Sophie, Pearce, Belinda, Wainwright, Denise, Mercer, Stuart J., Knight, Benjamin, Vijay, Vardhini, Alagaratnam, Swethan, Sinha, Sidhartha, Khan, Shahab, El-Hasani, Shamsi S., Hussain, Abdulzahra A., Bhattacharya, Vish, Kansal, Nisheeth, Fasih, Tani, Jackson, Claire, Siddiqui, Midhat N., Chishti, Imran A., Fordham, Imogen J., Siddiqui, Zohaib, Bausbacher, Harald, Geogloma, Ileana, Gurung, Kabita, Tsavellas, George, Basynat, Pradeep, Shrestha, Ashish Kiran, Basu, Sanjoy, Harilingam, Alok Chhabra Mohan, Rabie, Mohamed, Akhtar, Mansoor, Kumar, Pradeep, Jafferbhoy, Sadaf F., Hussain, Najam, Raza, Soulat, Haque, Manzarul, Alam, Imran, Aseem, Rabiya, Patel, Shakira, Asad, Mehek, Booth, Michael I., Ball, William R., Wood, Christopher P. J., Pinho-Gomes, Ana C., Kausar, Ambareen, Obeidallah, Moh’d Rami, Varghase, Joseph, Lodhia, Joshil, Bradley, Donal, Rengifo, Carla, Lindsay, David, Gopalswamy, Sivakumar, Finlay, Ian, Wardle, Stacy, Bullen, Naomi, Iftikhar, Syed Yusuf, Awan, Altaf, Ahmed, Javed, Leeder, Paul, Fusai, Guiseppe, Bond-Smith, Giles, Psica, Alicja, Puri, Yogesh, Hou, David, Noble, Fergus, Szentpali, Karoly, Broadhurst, Jack, Date, Ravindra, Hossack, Martin R., Goh, Yan Li, Turner, Paul, Shetty, Vinutha, Riera, Manel, Macano, Christina A. W., Sukha, Anisha, Preston, Shaun R., Hoban, Jennifer R., Puntis, Daniel J., Williams, Sophie V., Krysztopik, Richard, Kynaston, James, Batt, Jeremy, Doe, Matthew, Goscimski, Andrzej, Jones, Gareth H., Hall, Claire, Carty, Nick, Ahmed, Jamil, Panteleimonitis, Sofoklis, Gunasekera, Rohan T., Sheel, Andrea R. G., Lennon, Hannah, Hindley, Caroline, Reddy, Marcus, Kenny, Ross, Elkheir, Natalie, Mcglone, Emma R., Rajaganeshan, Rajasundaram, Hancorn, Kate, Hargreaves, Anita, Prasad, Raj, Longbotham, David A., Vijayanand, Dhakshinamoorthy, Wijetunga, Imeshi, Ziprin, Paul, Nicolay, Christopher R., Yeldham, Geoffrey, Read, Edward, Gossage, James A., Rolph, Rachel C., Ebied, Husam, Phull, Manraj, Khan, Mohammad A., Popplewell, Matthew, Kyriakidis, Dimitrios, Hussain, Anwar, Henley, Natasha, Packer, Jessica R., Derbyshire, Laura, Porter, Jonathan, Appleton, Shaun, Farouk, Marwan, Basra, Melvinder, Jennings, Neil A., Ali, Shahda, Kanakala, Venkatesh, Ali, Haythem, Lane, Risha, Dickson-Lowe, Richard, Zarsadias, Prizzi, Mirza, Darius, Puig, Sonia, Amari, Khalid Al, Vijayan, Deepak, Sutcliffe, Robert, Marudanayagam, Ravi, Hamady, Zayed, Prasad, Abheesh R., Patel, Abhilasha, Durkin, Damien, Kaur, Parminder, Bowen, Laura, Byrne, James P., Pearson, Katherine L., Delisle, Theo G., Davies, James, Tomlinson, Mark A., Johnpulle, Michelle A., Slawinski, Corinna, Macdonald, Andrew, Nicholson, James, Newton, Katy, Mbuvi, James, Farooq, Ansar, Mothe, Bhavani Sidhartha, Zafrani, Zakhi, Brett, Daniel, Francombe, James, Barnes, James, Cheung, Melanie, Al-Bahrani, Ahmed Z., Preziosi, Giuseppe, Urbonas, Tomas, Alberts, Justin, Mallik, Mekhlola, Patel, Krashna, Segaran, Ashvina, Doulias, Triantafyllos, Sufi, Pratik A., Yao, Caroline, Pollock, Sarah, Manzelli, Antonio, Wajed, Saj, Kourkulos, Michail, Pezzuto, Roberto, Wadley, Martin, Hamilton, Emma, Jaunoo, Shameen, Padwick, Robert, Sayegh, Mazin, Newton, Richard C., Hebbar, Madhusoodhana, Farag, Sameh F., Spearman, John, Hamdan, Mohammed F., D’Costa, Conrad, Blane, Christine, Giles, Mathew, Peter, Mark B., Hirst, Natalie A., Hossain, Tanvir, El-Dhuwaib, Arslan Pannu Yesar, Morrison, Tamsin E. M., Taylor, Greg W., Thompson, Ronald L. E., Mccune, Ken, Loughlin, Paula, Lawther, Roger, Byrnes, Colman K., Simpson, Duncan J., Mawhinney, Abi, Warren, Conor, Mckay, Damian, Mcilmunn, Colin, Martin, Serena, Macartney, Matthew, Diamond, Tom, Davey, Phil, Jones, Claire, Clements, Joshua M., Digney, Ruairi, Chan, Wei Ming, Mccain, Stephen, Gull, Sadaf, Janeczko, Adam, Dorrian, Emmet, Harris, Andrew, Dawson, Suzanne, Johnston, Dorothy, Mcaree, Barry, Ghareeb, Essam, Thomas, George, Connelly, Martin, Mckenzie, Stephen, Cieplucha, Krzysztos, Spence, Gary, Campbell, William, Hooks, Gareth, Bradley, Neil, Hill, Arnold D. K., Cassidy, John T., Boland, Michael, Burke, Paul, Nally, Deirdre M., Khogali, Elmoataz, Shabo, Wael, Iskandar, Edrin, Mcentee, Gerry P., O’Neill, Maeve A., Peirce, Colin, Lyons, Emma M., O’Sullivan, Adrian W., Thakkar, Rohan, Carroll, Paul, Ivanovski, Ivan, Balfe, Paul, Lee, Matthew, Winter, Des C., Kelly, Michael E., Hoti, Emir, Maguire, Donal, Karunakaran, Priyadarssini, Geoghegan, Justin G., Mcdermott, Frank, Martin, Sean T., Cross, Keith S., Cooke, Fiachra, Zeeshan, Saquib, Murphy, James O., Mealy, Ken, Mohan, Helen M., Nedujchelyn, Yuwaraja, Ullah, Muhammad Fahad, Ahmed, Irfan, Giovinazzo, Francesco, Milburn, James, Prince, Sarah, Brooke, Eleanor, Buchan, Joanna, Khalil, Ahmed M., Vaughan, Elizabeth M., Ramage, Michael I., Aldridge, Roland C., Gibson, Simon, Nicholson, Gary A., Vass, David G., Grant, Alan J., Holroyd, David J., Angharad Jones, M., Sutton, Cherith M. L. R., O’Dwyer, Patrick, Nilsson, Frida, Weber, Beatrix, Williamson, Tracey K., Lalla, Kushik, Bryant, Alice, Ross Carter, C., Forrest, Craig R., Hunter, David I., Nassar, Ahmad H., Orizu, Mavis N., Knight, Katrina, Qandeel, Haitham, Suttie, Stuart, Belding, Rowena, Mcclarey, Andrew, Boyd, Alan T., Guthrie, Graeme J. K., Lim, Pei J., Luhmann, Andreas, Watson, Angus J. M., Richards, Colin H., Nicol, Laura, Madurska, Marta, Harrison, Ewen, Boyce, Kathryn M., Roebuck, Amanda, Ferguson, Graeme, Pati, Pradeep, Wilson, Michael S. J., Dalgaty, Faith, Fothergill, Laura, Driscoll, Peter J., Mozolowski, Kirsty L., Banwell, Victoria, Bennett, Stephen P., Rogers, Paul N., Skelly, Brendan L., Rutherford, Claire L., Mirza, Ahmed K., Lazim, Taha, Lim, Henry C. C., Duke, Diana, Ahmed, Talat, Beasley, William D., Wilkinson, Marc D., Maharaj, Geta, Malcolm, Cathy, Brown, Timothy H., Al-Sarireh, Bilal, Shingler, Guy M., Mowbray, Nicholas, Radwan, Rami, Morcous, Paul, Wood, Simon, Kadhim, Abbas, Stewart, Duncan J., Baker, Andrew L., Tanner, Nicola, Shenoy, Hrishikesh, Hafiz, Shazia, De Marchi, Joshua A., Singh-Ranger, Deepak, Hisham, Elzanati, Ainley, Paul, John Terrace, Stephen O’Neill., Napetti, Sara, Hopwood, Benjamin, Rhys, Thomas, Downing, Justine, Kanavati, Osama, Coats, Maria, Aleksandrov, Danail, Kallaway, Charlotte, Yahya, Salama, Templeton, Alexa, Trotter, Martin, Christina, Lo, Dhillon, Ajit, Heywood, Nick, Aawsaj, Yousif, Hamdan, Alhafidz, Reece-Bolton, Obuobi, Mcguigan, Andrew, Shahin, Yousef, Aymon, Null, Luther, Ali Alison, Nicholson, James A., Rajendran, Ilayaraja, Boal, Matthew, and Ritchie, Judith

Subjects
Surgery
Published
2018

39. Myosins VIII and XI play distinct roles in reproduction and transport of tobacco mosaic virus

Author

Amari Khalid, Di Donato Martin, Dolja Valerian V., and Heinlein Manfred

Subjects
lcsh:Immunologic diseases. Allergy, Plant Cell Biology, viruses, Immunology, Plant Pathogens, RNA-dependent RNA polymerase, Plasmodesma, Plant Science, macromolecular substances, Biology, Myosins, Virus Replication, Microbiology, Virus, Plant Viral Pathogens, Motor protein, Virology, Plant virus, Myosin, Tobacco, Genetics, Tobacco mosaic virus, Molecular Biology, lcsh:QH301-705.5, Plasmodesmata, Biology and Life Sciences, Cell Biology, Plant Pathology, 3. Good health, Plant Viral Movement Proteins, Tobacco Mosaic Virus, Viral replication, lcsh:Biology (General), Parasitology, lcsh:RC581-607, Research Article
Abstract

Viruses are obligatory parasites that depend on host cellular factors for their replication as well as for their local and systemic movement to establish infection. Although myosin motors are thought to contribute to plant virus infection, their exact roles in the specific infection steps have not been addressed. Here we investigated the replication, cell-to-cell and systemic spread of Tobacco mosaic virus (TMV) using dominant negative inhibition of myosin activity. We found that interference with the functions of three class VIII myosins and two class XI myosins significantly reduced the local and long-distance transport of the virus. We further determined that the inactivation of myosins XI-2 and XI-K affected the structure and dynamic behavior of the ER leading to aggregation of the viral movement protein (MP) and to a delay in the MP accumulation in plasmodesmata (PD). The inactivation of myosin XI-2 but not of myosin XI-K affected the localization pattern of the 126k replicase subunit and the level of TMV accumulation. The inhibition of myosins VIII-1, VIII-2 and VIII-B abolished MP localization to PD and caused its retention at the plasma membrane. These results suggest that class XI myosins contribute to the viral propagation and intracellular trafficking, whereas myosins VIII are specifically required for the MP targeting to and virus movement through the PD. Thus, TMV appears to recruit distinct myosins for different steps in the cell-to-cell spread of the infection., Author Summary Viruses are parasites that require the host cell machinery for their propagation within and between cells. Myosins are molecular motors involved in the trafficking of cargos along actin filaments. Plant viruses have evolved to borrow this transport mechanism to aid their infection and spread within the plant. However, little is known about which of the many plant myosins are essential and at which specific steps they act to support virus infection. Here we investigated the role of different N. benthamiana myosins during the infection by Tobacco mosaic virus (TMV). Our results show that class XI myosins play specific roles in the reproduction and intracellular movement of TMV in association with the dynamic endoplasmic reticulum network, whereas class VIII myosins support the specific targeting of the viral movement protein to plasmodesmata and thus the cell-to-cell movement of the virus. Together these results indicate that TMV interacts with distinct myosins during specific infection steps.

Published
2014

40. Preoperative risk factors for conversion from laparoscopic to open cholecystectomy: a validated risk score derived from a prospective U.K. database of 8820 patients

Author

Sutcliffe, Robert P., primary, Hollyman, Marianne, additional, Hodson, James, additional, Bonney, Glenn, additional, Vohra, Ravi S., additional, Griffiths, Ewen A., additional, Fenwick, Stephen, additional, Elmasry, Mohamed, additional, Nunes, Quentin, additional, Kennedy, David, additional, Khan, Raja B., additional, Khan, Muhammad A.S., additional, Magee, Conor J., additional, Jones, Steven M., additional, Mason, Denise, additional, Parappally, Ciny P., additional, Mathur, Pawan, additional, Saunders, Michael, additional, Jamel, Sara, additional, Haque, Samer U.l., additional, Zafar, Sara, additional, Shiwani, Muhammad H., additional, Samuel, Nehemiah, additional, Dar, Farooq, additional, Jackson, Andrew, additional, Lovett, Bryony, additional, Dindyal, Shiva, additional, Winter, Hannah, additional, Rahman, Saquib, additional, Wheatley, Kevin, additional, Nieto, Tom, additional, Ayaani, Soofiyah, additional, Youssef, Haney, additional, Nijjar, Rajwinder S., additional, Watkin, Helen, additional, Naumann, David, additional, Emeshi, Sophie, additional, Sarmah, Piyush B., additional, Lee, Kathryn, additional, Joji, Nikita, additional, Heath, Jonathan, additional, Teasdale, Rebecca L., additional, Weerasinghe, Chamindri, additional, Needham, Paul J., additional, Welbourn, Hannah, additional, Forster, Luke, additional, Finch, David, additional, Blazeby, Jane M., additional, Robb, William, additional, McNair, Angus G.K., additional, Hrycaiczuk, Alex, additional, Charalabopoulos, Alexandros, additional, Kadirkamanathan, Sritharan, additional, Tang, Cheuk-Bong, additional, Jayanthi, Naga V.G., additional, Noor, Nigel, additional, Dobbins, Brian, additional, Cockbain, Andrew J., additional, Nilsen-Nunn, April, additional, de Siqueira, Jonathan, additional, Pellen, Mike, additional, Cowley, Jonathan B., additional, Ho, Wei-Min, additional, Miu, Victor, additional, White, Timothy J., additional, Hodgkins, Kathryn A., additional, Kinghorn, Alison, additional, Tutton, Matthew G., additional, Al-Abed, Yahya A., additional, Menzies, Donald, additional, Ahmad, Anwar, additional, Reed, Joanna, additional, Khan, Shabuddin, additional, Monk, David, additional, Vitone, Louis J., additional, Murtaza, Ghulam, additional, Joel, Abraham, additional, Brennan, Stephen, additional, Shier, David, additional, Zhang, Catherine, additional, Yoganathan, Thusidaran, additional, Robinson, Steven J., additional, McCallum, Iain J.D., additional, Jones, Michael J., additional, Elsayed, Mohammed, additional, Tuck, Liz, additional, Wayman, John, additional, Carney, Kate, additional, Aroori, Somaiah, additional, Hosie, Kenneth B., additional, Kimble, Adam, additional, Bunting, David M., additional, Fawole, Adeshina S., additional, Basheer, Mohammed, additional, Dave, Rajiv V., additional, Sarveswaran, Janahan, additional, Jones, Elinor, additional, Kendal, Chris, additional, Tilston, Michael P., additional, Gough, Martin, additional, Wallace, Tom, additional, Singh, Shailendra, additional, Downing, Justine, additional, Mockford, Katherine A., additional, Issa, Eyad, additional, Shah, Nayab, additional, Chauhan, Neal, additional, Wilson, Timothy R., additional, Forouzanfar, Amir, additional, Wild, Jonathan R.L., additional, Nofal, Emma, additional, Bunnell, Catherine, additional, Madbak, Khaliel, additional, Rao, Sudhindra T.V., additional, Devoto, Laurence, additional, Siddiqi, Najaf, additional, Khawaja, Zechan, additional, Hewes, James C., additional, Gould, Laura, additional, Chambers, Alice, additional, Rodriguez, Daniel U., additional, Sen, Gourab, additional, Robinson, Stuart, additional, Bartlett, Francis, additional, Rae, David M., additional, Stevenson, Thomas E.J., additional, Sarvananthan, Kas, additional, Dwerryhouse, Simon J., additional, Higgs, Simon M., additional, Old, Oliver J., additional, Hardy, Thomas J., additional, Shah, Reena, additional, Hornby, Steve T., additional, Keogh, Ken, additional, Frank, Lucinda, additional, Al-Akash, Musallam, additional, Upchurch, Emma A., additional, Frame, Richard J., additional, Hughes, Michael, additional, Jelley, Clare, additional, Weaver, Simon, additional, Roy, Sudipta, additional, Sillo, Toritseju O., additional, Galanopoulos, Giorgios, additional, Cuming, Tamzin, additional, Cunha, Pedro, additional, Tayeh, Salim, additional, Kaptanis, Sarantos, additional, Heshaishi, Mohamed, additional, Eisawi, Abdalla, additional, Abayomi, Michael, additional, Ngu, Wee S., additional, Fleming, Katie, additional, Bajwa, Dalvir S., additional, Chitre, Vivek, additional, Aryal, Kamal, additional, Ferris, Paul, additional, Silva, Michael, additional, Lammy, Simon, additional, Mohamed, Sarah, additional, Khawaja, Amir, additional, Hussain, Adnan, additional, Ghazanfar, Mudassar A., additional, Bellini, Maria I., additional, Ebdewi, Hamdi, additional, Elshaer, Mohamed, additional, Gravante, Gianpiero, additional, Drake, Benjamin, additional, Ogedegbe, Arikoge, additional, Mukherjee, Dipankar, additional, Arhi, Chanpreet, additional, Giwa, Lola, additional, Iqbal, Nusrat, additional, Watson, Nicholas F., additional, Aggarwal, Smeer K., additional, Orchard, Philippa, additional, Villatoro, Eduardo, additional, Willson, Peter D., additional, Mok, Kam W.J., additional, Woodman, Thomas, additional, Deguara, Jean, additional, Garcea, Giuseppe, additional, Babu, Benoy I., additional, Dennison, Alistair R., additional, Malde, Deep, additional, Lloyd, David, additional, Slavin, John P., additional, Jones, Robert P., additional, Ballance, Laura, additional, Gerakopoulos, Stratos, additional, Jambulingam, Periyathambi, additional, Mansour, Sami, additional, Sakai, Naomi, additional, Acharya, Vikas, additional, Sadat, Mohammed M., additional, Karim, Lawen, additional, Larkin, David, additional, Amin, Khalid, additional, Khan, Amarah, additional, Law, Jennifer, additional, Jamdar, Saurabh, additional, Smith, Stella R., additional, Sampat, Keerthika, additional, O'shea, Kathryn M., additional, Manu, Mangta, additional, Asprou, Fotini M., additional, Malik, Nabeela S., additional, Chang, Jessica, additional, Johnstone, Marianne, additional, Lewis, Michael, additional, Roberts, Geoffrey P., additional, Karavadra, Babu, additional, Photi, Evangelos, additional, Hewes, James, additional, Rodriguez, Dan, additional, O'Reilly, Derek A., additional, Rate, Anthony J., additional, Sekhar, Hema, additional, Henderson, Lucy T., additional, Starmer, Benjamin Z., additional, Coe, Peter O., additional, Tolofari, Sotonye, additional, Barrie, Jenifer, additional, Bashir, Gareth, additional, Sloane, Jake, additional, Madanipour, Suroosh, additional, Halkias, Constantine, additional, Trevatt, Alexander E.J., additional, Borowski, David W., additional, Hornsby, Jane, additional, Courtney, Michael J., additional, Virupaksha, Suvi, additional, Seymour, Keith, additional, Robinson, Sarah, additional, Hawkins, Helen, additional, Bawa, Sadiq, additional, Gallagher, Paul V., additional, Reid, Alistair, additional, Wood, Peter, additional, Finch, Jonathan G., additional, Finch, J.Guy, additional, Parmar, Jitesh, additional, Stirland, Euan, additional, Gardner-Thorpe, James, additional, Al-Muhktar, Ahmed, additional, Peterson, Mark, additional, Majeed, Ali, additional, Bajwa, Farrukh M., additional, Martin, Jack, additional, Choy, Alfred, additional, Tsang, Andrew, additional, Pore, Naresh, additional, Andrew, David R., additional, Al-Khyatt, Waleed, additional, Santosh Bhandari, Christopher Taylor, additional, Chambers, Adam, additional, Subramanium, Dhivya, additional, Toh, Simon K.C., additional, Carter, Nicholas C., additional, Mercer, Stuart J., additional, Knight, Benjamin, additional, Vijay, Vardhini, additional, Alagaratnam, Swethan, additional, Sinha, Sidhartha, additional, Khan, Shahab, additional, El-Hasani, Shamsi S., additional, Hussain, Abdulzahra A., additional, Bhattacharya, Vish, additional, Kansal, Nisheeth, additional, Fasih, Tani, additional, Jackson, Claire, additional, Siddiqui, Midhat N., additional, Chishti, Imran A., additional, Fordham, Imogen J., additional, Siddiqui, Zohaib, additional, Bausbacher, Harald, additional, Geogloma, Ileana, additional, Gurung, Kabita, additional, Tsavellas, George, additional, Basynat, Pradeep, additional, Shrestha, Ashish K., additional, Basu, Sanjoy, additional, Chhabra, Alok, additional, Harilingam, Mohan, additional, Rabie, Mohamed, additional, Akhtar, Mansoor, additional, Kumar, Pradeep, additional, Jafferbhoy, Sadaf F., additional, Hussain, Najam, additional, Raza, Soulat, additional, Haque, Manzarul, additional, Alam, Imran, additional, Aseem, Rabiya, additional, Patel, Shakira, additional, Asad, Mehek, additional, Booth, Michael I., additional, Ball, William R., additional, Wood, Christopher P.J., additional, Pinho-Gomes, Ana C., additional, Kausar, Ambareen, additional, Obeidallah, Mohammed, additional, Varghase, Joseph, additional, Lodhia, Joshil, additional, Bradley, Donal, additional, Rengifo, Carla, additional, Lindsay, David, additional, Gopalswamy, Sivakumar, additional, Finlay, Ian, additional, Wardle, Stacy, additional, Bullen, Naomi, additional, Iftikhar, Syed Y., additional, Awan, Altaf, additional, Ahmed, Javed, additional, Leeder, Paul, additional, Fusai, Guiseppe, additional, Bond-Smith, Giles, additional, Psica, Alicja, additional, Puri, Yogesh, additional, Hou, David, additional, Noble, Fergus, additional, Szentpali, Karoly, additional, Broadhurst, Jack, additional, Date, Ravindra, additional, Hossack, Martin R., additional, Goh, Yan L., additional, Turner, Paul, additional, Shetty, Vinutha, additional, Riera, Manel, additional, Macano, Christina A.W., additional, Sukha, Anisha, additional, Preston, Shaun R., additional, Hoban, Jennifer R., additional, Puntis, Daniel J., additional, Williams, Sophie V., additional, Krysztopik, Richard, additional, Kynaston, James, additional, Batt, Jeremy, additional, Doe, Matthew, additional, Goscimski, Andrzej, additional, Jones, Gareth H., additional, Hall, Claire, additional, Carty, Nick, additional, Ahmed, Jamil, additional, Panteleimonitis, Sofoklis, additional, Gunasekera, Rohan T., additional, Sheel, Andrea R.G., additional, Lennon, Hannah, additional, Hindley, Caroline, additional, Reddy, Marcus, additional, Kenny, Ross, additional, Elkheir, Natalie, additional, McGlone, Emma R., additional, Rajaganeshan, Rajasundaram, additional, Hancorn, Kate, additional, Hargreaves, Anita, additional, Prasad, Raj, additional, Longbotham, David A., additional, Vijayanand, Dhakshinamoorthy, additional, Wijetunga, Imeshi, additional, Ziprin, Paul, additional, Nicolay, Christopher R., additional, Yeldham, Geoffrey, additional, Read, Edward, additional, Gossage, James A., additional, Rolph, Rachel C., additional, Ebied, Husam, additional, Phull, Manraj, additional, Khan, Mohammad A., additional, Popplewell, Matthew, additional, Kyriakidis, Dimitrios, additional, Hussain, Anwar, additional, Henley, Natasha, additional, Packer, Jessica R., additional, Derbyshire, Laura, additional, Porter, Jonathan, additional, Appleton, Shaun, additional, Farouk, Marwan, additional, Basra, Melvinder, additional, Jennings, Neil A., additional, Ali, Shahda, additional, Kanakala, Venkatesh, additional, Ali, Haythem, additional, Lane, Risha, additional, Dickson-Lowe, Richard, additional, Zarsadias, Prizzi, additional, Mirza, Darius, additional, Puig, Sonia, additional, Al Amari, Khalid, additional, Vijayan, Deepak, additional, Sutcliffe, Robert, additional, Marudanayagam, Ravi, additional, Hamady, Zayed, additional, Prasad, Abheesh R., additional, Patel, Abhilasha, additional, Durkin, Damien, additional, Kaur, Parminder, additional, Bowen, Laura, additional, Byrne, James P., additional, Pearson, Katherine L., additional, Delisle, Theo G., additional, Davies, James, additional, Tomlinson, Mark A., additional, Johnpulle, Michelle A., additional, Slawinski, Corinna, additional, Macdonald, Andrew, additional, Nicholson, James, additional, Newton, Katy, additional, Mbuvi, James, additional, Farooq, Ansar, additional, Mothe, Bhavani S., additional, Zafrani, Zakhi, additional, Brett, Daniel, additional, Francombe, James, additional, Spreadborough, Philip, additional, Barnes, James, additional, Cheung, Melanie, additional, Al-Bahrani, Ahmed Z., additional, Preziosi, Giuseppe, additional, Urbonas, Tomas, additional, Alberts, Justin, additional, Mallik, Mekhlola, additional, Patel, Krashna, additional, Segaran, Ashvina, additional, Doulias, Triantafyllos, additional, Sufi, Pratik A., additional, Yao, Caroline, additional, Pollock, Sarah, additional, Manzelli, Antonio, additional, Wajed, Saj, additional, Kourkulos, Michail, additional, Pezzuto, Roberto, additional, Wadley, Martin, additional, Hamilton, Emma, additional, Jaunoo, Shameen, additional, Padwick, Robert, additional, Sayegh, Mazin, additional, Newton, Richard C., additional, Hebbar, Madhusoodhana, additional, Farag, Sameh F., additional, Hebbar, Madhu, additional, Spearman, John, additional, Hamdan, Mohammed F., additional, D'Costa, Conrad, additional, Blane, Christine, additional, Giles, Mathew, additional, Peter, Mark B., additional, Hirst, Natalie A., additional, Hossain, Tanvir, additional, Pannu, Arslan, additional, El-Dhuwaib, Yesar, additional, Morrison, Tamsin E.M., additional, Taylor, Greg W., additional, Thompson, Ronald L.E., additional, McCune, Ken, additional, Loughlin, Paula, additional, Lawther, Roger, additional, Byrnes, Colman K., additional, Simpson, Duncan J., additional, Mawhinney, Abi, additional, Warren, Conor, additional, McKay, Damian, additional, McIlmunn, Colin, additional, Martin, Serena, additional, MacArtney, Matthew, additional, Diamond, Tom, additional, Davey, Phil, additional, Jones, Claire, additional, Clements, Joshua M., additional, Digney, Ruairi, additional, Chan, Wei M., additional, McCain, Stephen, additional, Gull, Sadaf, additional, Janeczko, Adam, additional, Dorrian, Emmet, additional, Harris, Andrew, additional, Dawson, Suzanne, additional, Johnston, Dorothy, additional, McAree, Barry, additional, Ghareeb, Essam, additional, Thomas, George, additional, Connelly, Martin, additional, McKenzie, Stephen, additional, Cieplucha, Krzysztos, additional, Spence, Gary, additional, Campbell, William, additional, Hooks, Gareth, additional, Bradley, Neil, additional, Hill, Arnold D.K., additional, Cassidy, John T., additional, Boland, Michael, additional, Burke, Paul, additional, Nally, Deirdre M., additional, Khogali, Elmoataz, additional, Shabo, Wael, additional, Iskandar, Edrin, additional, McEntee, Gerry P., additional, O'Neill, Maeve A., additional, Peirce, Colin, additional, Lyons, Emma M., additional, O'Sullivan, Adrian W., additional, Thakkar, Rohan, additional, Carroll, Paul, additional, Ivanovski, Ivan, additional, Balfe, Paul, additional, Lee, Matthew, additional, Winter, Des C., additional, Kelly, Michael E., additional, Hoti, Emir, additional, Maguire, Donal, additional, Karunakaran, Priyadarssini, additional, Geoghegan, Justin G., additional, Martin, Sean T., additional, Cross, Keith S., additional, Cooke, Fiachra, additional, Zeeshan, Saquib, additional, Murphy, James O., additional, Mealy, Ken, additional, Mohan, Helen M., additional, Nedujchelyn, Yuwaraja, additional, Ullah, Muhammad F., additional, Ahmed, Irfan, additional, Giovinazzo, Francesco, additional, Milburn, James, additional, Prince, Sarah, additional, Brooke, Eleanor, additional, Buchan, Joanna, additional, Khalil, Ahmed M., additional, Vaughan, Elizabeth M., additional, Ramage, Michael I., additional, Aldridge, Roland C., additional, Gibson, Simon, additional, Nicholson, Gary A., additional, Vass, David G., additional, Grant, Alan J., additional, Holroyd, David J., additional, Jones, Angharad, additional, Sutton, Cherith M.L.R., additional, O'Dwyer, Patrick, additional, Nilsson, Frida, additional, Weber, Beatrix, additional, Williamson, Tracey K., additional, Lalla, Kushik, additional, Bryant, Alice, additional, Carter, Ross, additional, Forrest, Craig R., additional, Hunter, David I., additional, Nassar, Ahmad H., additional, Orizu, Mavis N., additional, Knight, Katrina, additional, Qandeel, Haitham, additional, Suttie, Stuart, additional, Belding, Rowena, additional, McClarey, Andrew, additional, Boyd, Alan T., additional, Guthrie, Graeme J.K., additional, Lim, Pei J., additional, Luhmann, Andreas, additional, Watson, Angus J.M., additional, Richards, Colin H., additional, Nicol, Laura, additional, Madurska, Marta, additional, Harrison, Ewen, additional, Boyce, Kathryn M., additional, Roebuck, Amanda, additional, Ferguson, Graeme, additional, Pati, Pradeep, additional, Wilson, Michael S.J., additional, Dalgaty, Faith, additional, Fothergill, Laura, additional, Driscoll, Peter J., additional, Mozolowski, Kirsty L., additional, Banwell, Victoria, additional, Bennett, Stephen P., additional, Rogers, Paul N., additional, Skelly, Brendan L., additional, Rutherford, Claire L., additional, Mirza, Ahmed K., additional, Lazim, Taha, additional, Lim, Henry C.C., additional, Duke, Diana, additional, Ahmed, Talat, additional, Beasley, William D., additional, Wilkinson, Marc D., additional, Maharaj, Geta, additional, Malcolm, Cathy, additional, Brown, Timothy H., additional, Shingler, Guy M., additional, Mowbray, Nicholas, additional, Radwan, Rami, additional, Morcous, Paul, additional, Wood, Simon, additional, Kadhim, Abbas, additional, Stewart, Duncan J., additional, Baker, Andrew L., additional, Tanner, Nicola, additional, and Shenoy, Hrishikesh, additional

Published
2016
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41. CDC48 function during TMV infection

Author

Niehl, Annette, Amari, Khalid, and Heinlein, Manfred

Subjects
Adenosine Triphosphatases, viruses, Cell Cycle Proteins, Virus Replication, Addendum, microtubules, protein inclusion, Tobacco Mosaic Virus, ER-assisted protein degradation, endoplasmic reticulum, Viral Proteins, Valosin Containing Protein, aggresome, virus infection, replication complex, Plant Proteins
Abstract

Cell-division-cycle protein 48 (CDC48) is an essential, conserved ATP-driven chaperone in eukaryotic cells, which functions in diverse cellular processes including the targeting of misfolded and aggregated proteins for degradation via proteasomal and aggresomal-autophagic pathways. We recently demonstrated that plant CDC48 localizes to and interacts with Tobacco mosaic virus (TMV) movement protein (MP) in ER-associated viral protein inclusions. Our data suggest that CDC48 participates in the clearance of these viral protein inclusions in an ER-assisted protein degradation (ERAD)-like mechanism. As TMV MP-inclusions formed at late infection stages resemble aggresomes, we here propose that TMV MP enters both, ERAD-like and aggresomal pathways in its host cells and that CDC48 coordinates these processes. Moreover, as viruses often exploit host pathways for replication and spread, we propose a model in which CDC48 functions in the degradation pathway of overaccumulating viral protein and also actively participates in the regulation of TMV replication and cell-to-cell movement.

Published
2012

43. Ilarviruses of Prunus spp: A Continued Concern for Fruit Trees

Author

Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Ministerio de Ciencia e Innovación, Dirección General de Investigación Científica y Técnica, Generalitat Valenciana, Pallás Benet, Vicente, Aparicio Herrero, Frederic, Herranz Gordo, Maria Del Carmen, Amari, Khalid, Sánchez Pina, Maria Amelia, Myrta, A., Sanchez Navarro, Jesus Angel, Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes, Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural, Ministerio de Ciencia e Innovación, Dirección General de Investigación Científica y Técnica, Generalitat Valenciana, Pallás Benet, Vicente, Aparicio Herrero, Frederic, Herranz Gordo, Maria Del Carmen, Amari, Khalid, Sánchez Pina, Maria Amelia, Myrta, A., and Sanchez Navarro, Jesus Angel

Abstract

[EN] Prunus spp. are affected by a large number of viruses, causing significant economic losses through either direct or indirect damage, which results in reduced yield and fruit quality. Among these viruses, members of the genus Ilarvirus (isometric labile ringspot viruses) occupy a significant position due to their distribution worldwide. Although symptoms caused by these types of viruses were reported early in the last century, their molecular characterization was not achieved until the 1990s, much later than for other agronomically relevant viruses. This was mainly due to the characteristic liability of virus particles in tissue extracts. In addition, ilarviruses, together with Alfalfa mosaic virus, are unique among plant viruses in that they require a few molecules of the coat protein in the inoculum in order to be infectious, a phenomenon known as genome activation. Another factor that has made the study of this group of viruses difficult is that infectious clones have been obtained only for the type member of the genus, Tobacco streak virus. Four ilarviruses, Prunus necrotic ringspot virus, Prune dwarf virus. Apple mosaic virus, and American plum line pattern virus, are pathogens of the main cultivated fruit trees. As stated in the 9th Report of the International Committee on Taxonomy of Viruses, virions of this genus are "unpromising subjects for the raising of good antisera." With the advent of molecular approaches for their detection and characterization, it has been possible to get a more precise view of their prevalence and genome organization. This review updates our knowledge on the incidence, genome organization and expression, genetic diversity, modes of transmission, and diagnosis, as well as control of this peculiar group of viruses affecting fruit trees.

Published
2012

44. A family of plasmodesmal proteins with receptor-like properties for plant viral movement proteins

Author

Amari, Khalid, Boutant, E., Hofmann, C., Schmitt-Keichinger, C., Fernandez-Calvino, L., Didier, P., Lerich, A., Mutterer, J., Thomas, C.L., Heinlein, Manfred, Mély, Y., Maule, A.J., Ritzenthaler, C., Amari, Khalid, Boutant, E., Hofmann, C., Schmitt-Keichinger, C., Fernandez-Calvino, L., Didier, P., Lerich, A., Mutterer, J., Thomas, C.L., Heinlein, Manfred, Mély, Y., Maule, A.J., and Ritzenthaler, C.

Abstract

Plasmodesmata (PD) are essential but poorly understood structures in plant cell walls that provide symplastic continuity and intercellular communication pathways between adjacent cells and thus play fundamental roles in development and pathogenesis. Viruses encode movement proteins (MPs) that modify these tightly regulated pores to facilitate their spread from cell to cell. The most striking of these modifications is observed for groups of viruses whose MPs form tubules that assemble in PDs and through which virions are transported to neighbouring cells. The nature of the molecular interactions between viral MPs and PD components and their role in viral movement has remained essentially unknown. Here, we show that the family of PD-located proteins (PDLPs) promotes the movement of viruses that use tubule-guided movement by interacting redundantly with tubule-forming MPs within PDs. Genetic disruption of this interaction leads to reduced tubule formation, delayed infection and attenuated symptoms. Our results implicate PDLPs as PD proteins with receptor-like properties involved the assembly of viral MPs into tubules to promote viral movement

Published
2010

46. An important new apricot disease in Spain is associated with Hop stunt viroid infection

Author

Ministerio de Economía y Competitividad (España), European Commission, Generalitat Valenciana, Consejo Superior de Investigaciones Científicas (España), Amari, Khalid, Ruiz, David, Gómez, Gustavo, Sánchez-Pina, M. Amelia, Pallás Benet, Vicente, Egea, José, Ministerio de Economía y Competitividad (España), European Commission, Generalitat Valenciana, Consejo Superior de Investigaciones Científicas (España), Amari, Khalid, Ruiz, David, Gómez, Gustavo, Sánchez-Pina, M. Amelia, Pallás Benet, Vicente, and Egea, José

Abstract

The province of Murcia (Murcia Region) is the one of the most important apricot growing regions in Europe. In recent years a fruit disorder named by growers as “degeneración” has been detected in apricot commercial orchards of this region, mainly in the variety Velázquez Fino. Affected fruits are characterized by changes in their external appearance involving rugosity and a loss of organoleptic characteristics, which leads to an unmarketable product. In order to identify the causal agent of this disorder, the presence of the most important viruses affecting stone fruit trees and Hop stunt viroid (HSVd) was tested. While negative results were obtained for all viruses analysed, the viroid was detected in all the symptomatic trees. Within a single tree, the viroid was restricted to branches bearing fruits with the characteristic symptoms but was usually absent from the rest of the tree. Sequence analysis of several isolates of HSVd obtained from these affected trees revealed the presence of two variants previously detected in other apricot cultivars. Taken together, these results suggest that degeneration is associated with HSVd.

Published
2007

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