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3 results on '"Alyssa C. Brown"'

1. The 50 Most Cited Articles on Meniscus Injuries and Surgery from 2000 to 2019 Focus on Arthroscopic Repair or Removal, Originate from Institutions Within the United States and Were Published Before 2010

Author

Alyssa C. Brown, M.P.H., Phara P. Ross, B.S., Symone M. Brown, M.P.H., and Mary K. Mulcahey, M.D.

Subjects
Sports medicine, RC1200-1245
Abstract

Purpose: To identify the 50 most cited original articles on meniscus injury and surgery from 2000 to 2019, and to perform a bibliometric analysis of the identified articles. Methods: A Clarivate Web of Science search, completed in June 2020, generated a list of the most cited articles related to meniscus research. Articles were sorted by number of times cited, and review articles or those unrelated to the meniscus were removed. Articles were classified as basic science or assigned the appropriate level of evidence. Extracted data included title, authors, journal, year of publication, country/institution of origin, total number of citations, and number of citations per year. Results: The final list of 50 included articles with a range of 106 to 490 citations and a mean of 162.34 total or 11.91 citations per year. The most cited articles appeared in 8 of the most influential journals in the field per the Journal Citation Index. Twenty-nine (58%) originated from institutions within the United States, and only 13 (26%) were published in 2010 or later. Overall, 25 (50%) were classified as therapeutic, only 5 (10%) were therapeutic randomized controlled studies, and 17 (34%) were basic science. “Arthroscopic meniscal repair or meniscectomy” appeared most frequently, with 16 (32%) falling into this subclassification. Conclusion: This study of the most cited meniscus articles showed a strong predominance for therapeutic studies, studies generated and published within the United States, and studies focused on topics of arthroscopic repair or removal. Overwhelmingly, included articles were published before 2010, affirming the criticism that bibliometric analysis favors older articles. Clinical relevance: This study provides information about which articles are driving the field relating to meniscus injuries and meniscus surgery in the last two decades.

Published
2021
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3. STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation

Author

Jonathan LaMarre, Marc-André Sirard, Allison Tscherner, Alyssa C Brown, Jennifer Kao, Leanne Stalker, and Isabelle Dufort

Subjects
STAT3 Transcription Factor, 0301 basic medicine, Gene Expression, lcsh:Medicine, Cycloheximide, Article, 03 medical and health sciences, chemistry.chemical_compound, Transcription (biology), Gene expression, Animals, STAT3, lcsh:Science, Cells, Cultured, Cumulus Cells, Multidisciplinary, biology, Chemistry, Activator (genetics), lcsh:R, Promoter, Cell biology, MicroRNAs, 030104 developmental biology, Oocytes, biology.protein, Cattle, lcsh:Q, Folliculogenesis, Signal transduction, Signal Transduction
Abstract

MicroRNAs are potent regulators of gene expression that have been widely implicated in reproduction and embryo development. Recent studies have demonstrated that miR-21, a microRNA extensively studied in the context of disease, is important in multiple facets of reproductive biology including folliculogenesis, ovulation, oocyte maturation and early mammalian development. Surprisingly, little is known about the mechanisms that regulate miR-21 and no studies have characterized these regulatory pathways in cumulus-oocyte complexes (COCs). We therefore investigated miR-21 in an in vitro model of bovine oocyte maturation. Levels of the primary transcript of miR-21 (pri-miR-21) and mature miR-21 increased markedly in COCs over the maturation period. Cloning of the bovine pri-miR-21 gene and promoter by 5′3′RACE (rapid amplification of cDNA ends) revealed a highly conserved region immediately upstream of the transcription start site and two alternatively-spliced variants of pri-miR-21. The promoter region contained several putative transcription factor binding sites, including two for signal transducer and activator of transcription 3 (STAT3). Mutation of these sites significantly decreased both the intrinsic activity of pri-miR-21 promoter-luciferase constructs and the response to leukemia inhibitory factor (LIF) (a STAT3 activator) in cultured MCF7 cells. In COCs, treatment with a STAT3 pathway inhibitor markedly decreased pri-miR-21 expression and prevented cumulus expansion. Pri-miR-21 expression was also inhibited by the protein synthesis inhibitor cycloheximide, suggesting that a protein ligand or signaling cofactor synthesized during maturation is necessary for transcription. Together these studies represent the first investigation of signaling pathways that directly influence miR-21 expression in bovine oocytes and cumulus cells.

Published
2018

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