36 results on '"Alvarado CZ"'
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2. The role of carcass chilling rate in the development of pale, exudative turkey pectoralis
- Author
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Alvarado, CZ, primary and Sams, AR, additional
- Published
- 2002
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3. Evaluation of growth production factors as predictors of the incidence and severity of white striping and woody breast in broiler chickens.
- Author
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Aguirre ME, Leyva-Jimenez H, Travis R, Lee JT, Athrey G, and Alvarado CZ
- Subjects
- Animals, Housing, Animal, Incidence, Muscular Diseases epidemiology, Muscular Diseases pathology, Poultry Diseases pathology, Risk Factors, Texas epidemiology, Animal Husbandry methods, Chickens, Muscular Diseases veterinary, Pectoralis Muscles pathology, Poultry Diseases epidemiology
- Abstract
White striping (WS) and woody breast (WB) have been previously associated with older and heavier birds. However, there is limited information supporting the association between these 2 muscle conditions and growth parameters. The objectives of this study were 1) to investigate the relationship between WS and WB using different growth production factors and 2) to propose a predictive model that uses growth production factors to investigate the incidence and severity of WS and WB. A combined database of 4,332 broilers pooled from 7 research experiments conducted from 2016 to 2017 at Texas A&M University was used in this study. Parameters such as sex, age (4 wk, 6 wk, and 8 wk), strain (standard A vs. high-breast-yield [B and C]), live weight categories (500 g increments), and breast weight categories (250 g increments) were included in the model. Results showed that WS was 12% more likely to be present in non-WB fillets. The association between WS and WB suggests a moderate relationship between the ranks of both outcome variables (ρ = 0.57, P < 0.0001). Variables such as age, live weight, and sex were not as important as breast weight and strain in the severity prediction of WS and WB. Butterfly fillets above 750 g and with high-breast-yielding strains were more likely associated with higher severity of WS and WB scores. No post hoc variable selection was performed. Both models show good discrimination. The WS model produced an uncorrected area under the curve (AUC) of 0.739, with a bootstrap corrected estimate of 0.736. The WB model produced an uncorrected AUC of 0.753 and a bootstrap corrected estimate of 0.752. Therefore, the growth production factors analyzed in this study indicated that there is a moderate relationship between WS and WB myopathies and were jointly predictive of the severity of WS and WB. Potentially other factors not included in this study may play a major role in the relationship of these 2 myopathies. More research should be done to investigate this possibility., (Copyright © 2020. Published by Elsevier Inc.)
- Published
- 2020
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4. Insights into the Identification of the Specific Spoilage Organisms in Chicken Meat.
- Author
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Saenz-García CE, Castañeda-Serrano P, Mercado Silva EM, Alvarado CZ, and Nava GM
- Abstract
Poultry meat deterioration is caused by environmental conditions, as well as proliferation of different bacterial groups, and their interactions. It has been proposed that meat spoilage involves two bacterial groups: one group that initiates the deterioration process, known as specific spoilage organisms (SSOs), and the other known as spoilage associated organisms (SAOs) which represents all bacteria groups recovered from meat samples before, during, and after the spoilage process. Numerous studies have characterized the diversity of chicken meat SAOs; nonetheless, the identification of the SSOs remains a long-standing question. Based on recent genomic studies, it is suggested that the SSOs should possess an extensive genome size to survive and proliferate in raw meat, a cold, complex, and hostile environment. To evaluate this hypothesis, we performed comparative genomic analyses in members of the meat microbiota to identify microorganisms with extensive genome size and ability to cause chicken meat spoilage. Our studies show that members of the Pseudomonadaceae family have evolved numerous biological features such as large genomic size, slow-growing potential, low 16S rRNA copy number, psychrotrophic, and oligotrophic metabolism to initiate the spoilage of poultry meat. Moreover, inoculation experiments corroborated that these biological traits are associated with the potential to cause chicken meat deterioration. Together, these results provide new insights into the identification of SSO. Further studies are in progress to elucidate the impact of the SSO on meat quality and microbiota diversity.
- Published
- 2020
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5. Sanitization of Chicken Frames by a Combination of Hydrogen Peroxide and UV Light To Reduce Contamination of Derived Edible Products.
- Author
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Jones-Ibarra AM, Alvarado CZ, Coufal CD, and Taylor TM
- Subjects
- Animals, Colony Count, Microbial, Humans, Salmonella enteritidis drug effects, Salmonella enteritidis radiation effects, Salmonella typhimurium drug effects, Salmonella typhimurium radiation effects, Chickens, Food Microbiology methods, Hydrogen Peroxide pharmacology, Ultraviolet Rays
- Abstract
Chicken carcass frames are used to obtain mechanically separated chicken (MSC) for use in other further processed food products. Previous foodborne disease outbreaks involving Salmonella- contaminated MSC have demonstrated the potential for the human pathogen to be transmitted to consumers via MSC. The current study evaluated the efficacy of multiple treatments applied to the surfaces of chicken carcass frames to reduce microbial loads on noninoculated frames and frames inoculated with a cocktail of Salmonella enterica serovar Enteritidis and Salmonella enterica serovar Typhimurium. Inoculated or noninoculated frames were left untreated (control) or were subjected to treatment using a prototype sanitization apparatus. Treatments consisted of (i) a sterile water rinse, (ii) a water rinse followed by 5 s of UV-C light application, or (iii) an advanced oxidation process (AOP) combining 5 or 7% (v/v) hydrogen peroxide (H
2 O2 ) with UV-C light. Treatment with 7% H2 O2 and UV-C light reduced numbers of aerobic bacteria by up to 1.5 log CFU per frame ( P < 0.05); reductions in aerobic bacteria subjected to other treatments did not statistically differ from one another (initial mean load on nontreated frames: 3.6 ± 0.1 log CFU per frame). Salmonella numbers (mean load on inoculated, nontreated control was 5.6 ± 0.2 log CFU per frame) were maximally reduced by AOP application in comparison with other treatments. No difference in Salmonella reductions obtained by 5% H2 O2 (1.1 log CFU per frame) was detected compared with that obtained following 7% H2 O2 use (1.0 log CFU per frame). The AOP treatment for sanitization of chicken carcass frames reduces microbial contamination on chicken carcass frames that are subsequently used for manufacture of MSC.- Published
- 2019
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6. Salmonella recovery from chicken bone marrow and cecal counts differ by pathogen challenge method.
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Jones-Ibarra AM, Alvarado CZ, Caldwell DH, Byrd JA, and Taylor TM
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- Administration, Cutaneous, Administration, Inhalation, Administration, Oral, Animals, Female, Male, Random Allocation, Bone Marrow microbiology, Cecum microbiology, Chickens, Poultry Diseases microbiology, Salmonella Infections, Animal microbiology, Salmonella enterica isolation & purification
- Abstract
Mechanically separated chicken (MSC) may be incorporated into other further processed foods, and has been identified as a transmission vehicle in human foodborne disease outbreaks involving the pathogen Salmonella enterica. Chickens possess pneumatic bones producing marrow, which may become infected with Salmonella and present a mechanism for Salmonella contamination of MSC. The primary objective of the current study was to ascertain whether chicken bone marrow was susceptible to Salmonella infection as a function of pathogen challenge method. Additionally, this study sought to determine the impact of Salmonella challenge site and inoculation on subsequent numbers of the microorganism in the cecum at 3, 6 and 9 d post-challenge. In this study, 30-day-old birds (Gallus domesticus) were separately challenged with S. enterica by one of the 6 experimental treatment methods: oral or endo-tracheal gavage, and transdermal scratch challenge on the breast or back muscles, with or without feathers (N = 150). Differing Salmonella recovery rates were detected in bone marrow from euthanized birds (P = 0.0015); oral and endo-tracheal gavage produced Salmonella recovery frequencies of 10% and 20%, respectively. Counts of Salmonella from chicken cecal samples statistically differed as a function of challenge method (P = 0.032); the highest numbers of Salmonella in cecal samples were obtained from orally gavaged birds (3.0 log10 cfu/g). Study results demonstrate the potential for chickens to contract short-term systemic infection following Salmonella challenge using methods of pathogen challenge simulating aerosol inhalation, ingestion, or wounding. Salmonella entry into chicken bone marrow may result in decreased likelihood of Salmonella performance standard compliance and increased pathogen transmission risk to consumers., (Published by Oxford University Press on behalf of Poultry Science Association 2019.)
- Published
- 2019
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7. Descriptive sensory and instrumental texture profile analysis of woody breast in marinated chicken.
- Author
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Aguirre ME, Owens CM, Miller RK, and Alvarado CZ
- Subjects
- Animals, Chickens, Color, Food Handling methods, Meat analysis, Pectoralis Muscles chemistry
- Abstract
The broiler industry is currently experiencing a muscle anomaly referred to as "woody breast," and the effect of different cooking methods on the marination properties of severe woody breast (SWB) has not yet been reported. This study compared the texture attributes of marinated (injected) normal (NOR) and SWB using a convection oven and a flat-top grill. The objectives were 1) to develop and validate a descriptive texture attribute panel with 6 trained panelists using a 16-point scale and 2) to evaluate the instrumental texture profile analysis (TPA) using a texture analyzer. Sixty-four NOR and SWB were obtained from a commercial facility. Fillet color (L*, a*, b*) and pH were measured before marination. In each of 2 trials, the breast muscles were injected in bulk with 15% brine (0.48 STPP, 0.55% NaCl, final concentration), and marinade retention was determined after 20 minutes. The meat was vacuum packaged, stored at -20°C (7 d sensory; 29 d TPA) and then thawed (4°C, 24 h). Fillets were cooked to 73°C on a flat-top grill (176°C) or in an oven (176°C), and cook loss % was determined. Panelist samples (2 × 2 cm) and TPA samples (4 × 2 cm) were cut into 3 pieces. Color and pH were higher for SWB than NOR fillets (P < 0.05). Marinade retention was 83.21% for NOR meat and 59.23% for SWB meat. The flat-top grill method resulted in higher cook loss than oven (P < 0.05). SWB had higher cook loss when compared to NOR (P < 0.05). Sensory texture descriptors springiness, hardness, denseness, cohesiveness, fracturability, fibrousness, crunchiness, and chewiness were higher for SWB than NOR fillets (P < 0.05). TPA attributes also showed higher values for SWB compared to NOR (P < 0.05). No differences in texture were found between the grill and oven for sensory and TPA attributes. In summary, marinated SWB has significant texture differences when compared to NOR, regardless of cooking method.
- Published
- 2018
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8. Utilizing original XPC™ in feed to reduce stress susceptibility of broilers.
- Author
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Price PT, Byrd JA, Alvarado CZ, Pavlidis HO, McIntyre DR, and Archer GS
- Subjects
- Animal Feed analysis, Animals, Random Allocation, Chickens physiology, Diet veterinary, Dietary Supplements analysis, Hot Temperature adverse effects, Stress, Physiological
- Abstract
Reduction of stress is an important factor in improving poultry welfare, especially during periods of heat stress. A study was conducted to evaluate the effects of feeding the functional metabolites of Diamond V Original XPCTM to broilers reared under ambient or heat stress temperatures. Dietary treatments included: control feed (CON) and Original XPC fed continuously at 1.25 kg/MT (XPC). Half the birds in each dietary treatment were subjected to either no heat stress (24°C constant) or heat stress (35°C:24°C for 18:6 h daily) from 28 to 42 d. At the end of the heat stress period, blood was collected from 40 birds/treatment. Blood was analyzed for plasma corticosterone (CORT), plasma HSP70 (HSP70), and heterophil/lymphocyte ratios (H/L). At 42 d, bilateral metatarsal traits were also measured in 40 birds/treatment to assess physical asymmetry. Birds fed XPC had significantly lower CORT levels than CON (P < 0.001; 5,129 ± 617 vs. 8,433 ± 730, respectively). Physical asymmetry scores were also significantly higher in CON compared to XPC fed broilers (P < 0.001; 1.50 ± 0.13 vs. 0.54 ± 0.05, respectively). H/L ratios were significantly greater in CON than for XPC birds (P = 0.01; 0.81 ± 0.05 mm vs. 0.62 ± 0.05 mm, respectively). No differences were observed between CON and XPC fed broilers in HSP70. However, heat stress did increase (P < 0.0001) HSP70 compared to no heat stress birds (5.65 ± 0.12 vs. 4.78 ± 0.11 pg/mL, for heat stress and no heat stress, respectively). Feeding XPC to broiler chickens improved animal welfare via reduced stress indicators while under heat stress or no heat stress conditions. These results indicate that feeding XPC may improve poultry welfare by reducing heat stress susceptibility.
- Published
- 2018
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9. Comparative evaluation of texture wheat ingredients and soy proteins in the quality and acceptability of emulsified chicken nuggets.
- Author
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Yeater M, Casco G, Miller RK, and Alvarado CZ
- Subjects
- Animals, Chickens, Emulsions analysis, Food Handling methods, Soybean Proteins analysis, Triticum chemistry, Consumer Behavior, Food Quality, Meat Products analysis, Plant Proteins analysis
- Abstract
Chicken nuggets are commonly made with varying levels of textured vegetable proteins, such as soy and wheat, for their ability to bind water and their meat like conformation. This project compared textured wheat proteins and soy proteins at 10%, 20%, 30%, and 40% in emulsified chicken nuggets. A total of 3,024 chicken nuggets were evaluated in replications for batter breader pickup (%), par fry yield (%), cook loss (%), L*, a*, b* color value, texture profile analysis, and sensory analysis. The analysis was conducted for all 4 concentrations of wheat and soy treatments then compared to each other and an all white meat chicken nugget control. All data were analyzed with an α < 0.05 using SAS with PROC GLM and Duncan's MRT, except for sensory data, which were analyzed as a complete randomized block design using analysis of variance with a α < 0.05 and was analyzed using SAS with PROC GLM. Chicken nuggets prepared with increasing levels of textured soy and wheat proteins exhibited generally similar properties in terms of yields, color, and objective texture. Trained panel sensory analysis indicated an increased detection of soy flavors over wheat flavors at higher inclusion percentages (30% and 40%); however, these results do not have any implication of consumer acceptance., (© 2017 Poultry Science Association Inc.)
- Published
- 2017
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10. Validation of Thermal Lethality against Salmonella enterica in Poultry Offal during Rendering.
- Author
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Jones-Ibarra AM, Acuff GR, Alvarado CZ, and Taylor TM
- Subjects
- Animals, Colony Count, Microbial, Food Microbiology, Humans, Salmonella, Food Contamination analysis, Food Handling methods, Poultry microbiology, Salmonella enterica growth & development
- Abstract
Recent outbreaks of human disease following contact with companion animal foods cross-contaminated with enteric pathogens, such as Salmonella enterica, have resulted in increased concern regarding the microbiological safety of animal foods. Additionally, the U.S. Food and Drug Administration Food Safety Modernization Act and its implementing rules have stipulated the implementation of current good manufacturing practices and food safety preventive controls for livestock and companion animal foods. Animal foods and feeds are sometimes formulated to include thermally rendered animal by-product meals. The objective of this research was to determine the thermal inactivation of S. enterica in poultry offal during rendering at differing temperatures. Raw poultry offal was obtained from a commercial renderer and inoculated with a mixture of Salmonella serovars Senftenberg, Enteritidis, and Gallinarum (an avian pathogen) prior to being subjected to heating at 150, 155, or 160°F (65.5, 68.3, or 71.1°C) for up to 15 min. Following heat application, surviving Salmonella bacteria were enumerated. Mean D-values for the Salmonella cocktail at 150, 155, and 160°F were 0.254 ± 0.045, 0.172 ± 0.012, and 0.086 ± 0.004 min, respectively, indicative of increasing susceptibility to increased application of heat during processing. The mean thermal process constant (z-value) was 21.948 ± 3.87°F. Results indicate that a 7.0-log-cycle inactivation of Salmonella may be obtained from the cumulative lethality encountered during the heating come-up period and subsequent rendering of raw poultry offal at temperatures not less than 150°F. Current poultry rendering procedures are anticipated to be effective for achieving necessary pathogen control when completed under sanitary conditions.
- Published
- 2017
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11. Meat quality of broiler breast fillets with white striping and woody breast muscle myopathies.
- Author
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Tijare VV, Yang FL, Kuttappan VA, Alvarado CZ, Coon CN, and Owens CM
- Subjects
- Age Factors, Animals, Food Handling, Male, Muscular Diseases pathology, Pectoralis Muscles pathology, Chickens, Meat analysis, Meat standards, Muscular Diseases veterinary, Poultry Diseases pathology
- Abstract
The global poultry industry has been faced with emerging broiler breast meat quality issues including conditions known as white striping (WS, white striations parallel to muscle fibers) and woody breast (WB, hardness of raw fillet). Experiments were conducted to evaluate effects of WS and WB hardness on meat quality traits in broiler breast fillets. In Exp. 1, birds were processed at approximately 9 wk of age and deboned at 4 h postmortem (PM); in Exp. 2, birds were processed at approximately 6 and 9 wk of age and deboned at 2 h PM. Fillets were categorized as: normal for both white striping and woody breast (NORM); moderate for white striping and mild for woody breast (MILD); severe for white striping and mild for woody breast (WS); severe for woody breast and moderate for white striping (WB); or severe for both white striping and woody breast (BOTH). Sarcomere length, gravimetric fragmentation index, marination uptake, cook loss, and Meullenet-Owens razor shear energy (MORSE) values on non-marinated and marinated fillets were assessed. Sarcomeres tended to be longer (P = 0.07) with increasing severity of WS and WB in both experiments and gravimetric fragmentation index did not differ (P > 0.05) among categories. Marinade uptake decreased (P < 0.05) with increasing severity of WS and WB. Cook losses of non-marinated and marinated fillets were greatest (P < 0.05) in the BOTH category. Even though MORSE values did not differ (P > 0.05) in non-marinated fillets, the marinated BOTH fillets had greater MORSE values (P < 0.05) than other categories of fillets in Exp. 1. Non-marinated NORM fillets had greater (P < 0.05) MORSE values than the other categories at 6 wk age; however, MORSE values did not differ (P > 0.05) among categories of marinated breasts. At 9 wk, WS and BOTH were higher (P < 0.05) in MORSE compared to NORM for non-marinated fillets, but similar to NORM marinated fillets. Results suggest that severe degrees of white striping and woody breast, individually or in combination, negatively impact meat quality, especially water holding capacity attributes such as marinade uptake and cook loss., (© 2016 Poultry Science Association Inc.)
- Published
- 2016
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12. Comparison of eggshell surface sanitization technologies and impacts on consumer acceptability.
- Author
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Al-Ajeeli MN, Taylor TM, Alvarado CZ, and Coufal CD
- Subjects
- Animals, Bacteria, Aerobic isolation & purification, Chickens, Chlorine pharmacology, Eggs standards, Food Handling, Hydrogen Peroxide pharmacology, Quaternary Ammonium Compounds pharmacology, Ultraviolet Rays, Bacteria, Aerobic drug effects, Disinfectants pharmacology, Egg Shell microbiology, Eggs microbiology, Food Microbiology, Salmonella enteritidis drug effects
- Abstract
Shell eggs can be contaminated with many types of microorganisms, including bacterial pathogens, and thus present a risk for the transmission of foodborne disease to consumers. Currently, most United States egg processors utilize egg washing and sanitization systems to decontaminate surfaces of shell eggs prior to packaging. However, previous research has indicated that current shell egg sanitization technologies employed in the commercial egg industry may not completely eliminate bacteria from the surface of eggshells, and thus alternative egg sanitization technologies with the potential for increased microbial reductions on eggshells should be investigated. The objectives of this study were to compare the antimicrobial efficacy and consumer sensory attributes of industry-available eggshell sanitization methods (chlorine and quaternary ammonium compounds (QAC) applied via spray) to various alternative egg sanitization technologies. Eggs (White Leghorn hens; n=195) were obtained for evaluation of sanitizer-induced reduction in mesophilic aerobic bacteria (n=90) or inoculated Salmonella Enteritidis (SE) reduction (n=105). Sanitizing treatments evaluated in this experiment were: chlorine spray (100 ppm available chlorine), QAC spray (200 ppm), peracetic acid spray (PAA; 135 ppm) alone or in combination with ultraviolet light (UV; 254 nm), and hydrogen peroxide (H2O2; 3.5% solution) spray in combination with UV (H2O2+UV). For enumeration of aerobic bacteria, eggs were sampled at 0, 7, and 14 days of storage at 4°C; surviving SE cells from inoculated eggs were enumerated by differential plating. Sensory trials were conducted to determine consumer liking of scrambled eggs made from eggs sanitized with chlorine, QAC, H2O2+UV, or no treatment (control). The H2O2 and UV treatment resulted in the greatest reductions in eggshell aerobic plate counts compared to other treatments throughout egg storage (P<0.05). All treatments utilized reduced SE below the limit of detection by eggshell rinse. There were no differences in consumers' liking of overall flavor between the 4 treatments evaluated. The application of H2O2+UV treatment to shell eggs represents a novel technology that could have important implications for egg quality and safety preservation., (© 2016 Poultry Science Association Inc.)
- Published
- 2016
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13. Transport Mechanisms and Quality Changes During Frying of Chicken Nuggets--Hybrid Mixture Theory Based Modeling and Experimental Verification.
- Author
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Bansal HS, Takhar PS, Alvarado CZ, and Thompson LD
- Subjects
- Animals, Chickens, Color, Elasticity, Humans, Hydrophobic and Hydrophilic Interactions, Kinetics, Meat standards, Pressure, Reproducibility of Results, Cooking, Hot Temperature, Meat analysis, Models, Theoretical, Oils chemistry, Triticum, Water chemistry
- Abstract
Hybrid mixture theory (HMT) based 2-scale fluid transport relations of Takhar coupled with a multiphase heat transfer equation were solved to model water, oil and gas movement during frying of chicken nuggets. A chicken nugget was treated as a heterogeneous material consisting of meat core with wheat-based coating. The coupled heat and fluid transfer equations were solved using the finite element method. Numerical simulations resulted in data on spatial and temporal profiles for moisture, rate of evaporation, temperature, oil, pore pressure, pressure in various phases, and coefficient of elasticity. Results showed that most of the oil stayed in the outer 1.5 mm of the coating region. Temperature values greater than 100 °C were observed in the coating after 30 s of frying. Negative gage-pore pressure (p(w) < p(g)) magnitudes were observed in simulations, which is in agreement with experimental observations of Sandhu and others. It is hypothesized that high water-phase capillary pressure (p(c) > p(g)) in the hydrophilic matrix causes p(w) < p(g), which further results in negative pore pressure. The coefficient of elasticity was the highest at the surface (2.5 × 10(5) Pa) for coating and the interface of coating and core (6 × 10(5) Pa). Kinetics equation for color change obtained from experiments was coupled with the HMT based model to predict the color (L, a, and b) as a function of frying time., (© 2015 Institute of Food Technologists®)
- Published
- 2015
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14. Controlling Listeria monocytogenes Scott A on Surfaces of Fully Cooked Turkey Deli Product Using Organic Acid-Containing Marinades as Postlethality Dips.
- Author
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Casco G, Johnson JL, Taylor TM, Gaytán CN, Brashears MM, and Alvarado CZ
- Abstract
This study evaluated the efficacy of organic acids applied singly or in combination as postlethality dips to sliced uncured turkey deli loaves to inhibit the growth of Listeria monocytogenes (Lm) Scott A. Treatments consisted of sodium lactate (SL; 3.6%), potassium lactate (PL; 3.6%), sodium citrate (SC; 0.75%), a combination of SL and sodium diacetate (SDA; 0.25%), and a combination of SL/PL/SDA, alongside appropriate negative and positive controls. Products were inoculated with 10(4)-10(5) CFU/mL streptomycin-resistant (1500 μg/mL) Lm Scott A prior to treatment. Products were then stored at ~4°C and sampled at 0, 7, 14, 21, 28, 42, and 56 d. The SL/SDA combination applied to turkey slices extended the lag phase through 21 days of refrigerated storage. Numbers of Lm Scott A rose by 0.7 log10 CFU/g through the 56 d storage period. The application of the SL/PL/SDA treatment to turkey product surfaces extended the lag phase through 42 d, with pathogen numbers declining after 21 d. Combination organic acid dips prolonged the lag phase for 2 to 6 wk on turkey product surfaces and can be useful as antimicrobial agents for Lm control on postlethality exposed sliced deli products.
- Published
- 2015
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15. SavorPhos as an all-natural phosphate replacer in water- and oil-based marinades for rotisserie birds and boneless-skinless breast.
- Author
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Casco G, Veluz GA, and Alvarado CZ
- Subjects
- Animals, Cooking, Plant Oils analysis, Random Allocation, Sodium Chloride analysis, Water analysis, Chickens physiology, Food Handling, Meat standards, Pectoralis Muscles physiology, Phosphates analysis
- Abstract
As consumer demand for all-natural marinades increases, the need to replace phosphate with a natural product that can produce equivalent or improved yield in products such as but not limited to rotisserie chickens (whole birds without giblets) and boneless/skinless breast (BSB) is a challenge for processors. The objective of this study was to determine if using an all-natural nonphosphate blend (SavorPhos-200, SP) in water-based (WB) and oil-based (OB) marinades would perform better than a commercial phosphate blend (PB). The treatments included WB+PB (water, 0.4% phosphate, 0.7% salt), WB+SP (water, 0.5% SavorPhos-200, 0.7% salt), OB+PB (water, 3% oil, 0.4% phosphate, 0.7% salt), and OB+SP (water, 3% oil, 0.5% SavorPhos-200, 0.7% salt). The rotisserie chickens and BSB were injected with a multineedle injector to 20% (wt/wt) pickup at a constant pressure (103-138 kPa). The parameters measured were marinade pickup %, 20-min and 24-h marinade retention %, and cook loss %. Color, tenderness, total moisture, and sensory test were conducted on BSB. Data were analyzed within marinade type (WB and OB). Rotisserie birds picked up and retained the same yield in WB marinades. In OB marinades, SP had higher yields postinjection and lower cook loss % than the PB, while retaining the same yield over 24 h. For BSB, the cook loss was lower in SP than the PB in WB marinades. Higher yields postinjection were achieved with OB, but had the same retention 20 min and 24 h postinjection and cook loss % as the PB. No differences were observed for total moisture or L* (lightness) within marinade type and treatment. Texture was lower, indicating increased tenderness (P < 0.05) on SP samples in both marinades. However, consumers were not able to distinguish between treatments in sensory analyses. Therefore, SavorPhos-200 can be used as a natural nonphosphate blend in WB marinades with no detriment to yield. In addition, SavorPhos-200 can be used as a natural nonphosphate blend in OB marinades with yield improvements.
- Published
- 2013
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16. Marination and packaging impact on textural properties of home-frozen broiler breast fillets.
- Author
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Sanchez-Peña AG and Alvarado CZ
- Subjects
- Animals, Cooking, Pectoralis Muscles physiology, Random Allocation, Time Factors, Chickens physiology, Food Handling, Food Packaging, Food Storage, Freezing, Meat standards
- Abstract
Different packaging is available to consumers, and marination is widely applied. However, their effects on the quality of broiler breast fillets during home freezing are not well known to consumers. Therefore, the objective of this study was to evaluate the effect of packaging materials on the quality of chicken breast fillets after 1, 3, and 6 wk storage at -18°C. A total of 256 fillet were randomly placed in tray packs, freezer bags, butcher paper, and nonpackaged controls. Samples were analyzed for raw pH, color, percent moisture content, and TBA reactive substances (TBARS) at d 0 (only nonpackaged controls), wk 1, 3, and 6, and cooked texture and sensory attributes at wk 1 and 6. There were differences (P < 0.05) in pH, color, percent moisture content, and texture among the treatments by wk 6, but no differences (P < 0.05) in TBARS values and the sensory evaluation (tenderness, juiciness, and overall flavor) were seen. Consumers were not able to detect quality differences (P < 0.05) among treatments despite analytical differences. Experiment 2 was conducted to evaluate the effects of marination on the quality of chicken breast fillets after 2 wk of storage in a home freezer (-18°C). A total of 240 fillets were used in the treatments: nonmarinated fresh nonfrozen, nonmarinated frozen, marinated fresh nonfrozen, and marinated frozen. Fillets were vacuum-tumbled in a 10% solution yielding a final concentration of 0.45% sodium tripolyphosphate and 0.65% salt in the meat. Raw samples were evaluated for color, TBARS, and thaw loss. Cooked samples after 2 wk of storage were analyzed for cook loss, texture, moisture, and sensory evaluation (color, juiciness, tenderness, overall flavor, and preference). Both marinated samples showed better (P < 0.05) quality in terms of L* value, TBARS, cook loss, texture, moisture content, and better sensory attributes (except color) than nonmarinated samples. Quality of marinated samples was not negatively affected by home freezing after 2 wk.
- Published
- 2013
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17. Quality and safety of broiler meat in various chilling systems.
- Author
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Demirok E, Veluz G, Stuyvenberg WV, Castañeda MP, Byrd A, and Alvarado CZ
- Subjects
- Animals, Campylobacter isolation & purification, Colony Count, Microbial, Random Allocation, Salmonella isolation & purification, Time Factors, Chickens, Cold Temperature, Food Microbiology, Food Quality, Meat microbiology, Meat standards, Meat-Packing Industry methods
- Abstract
Chilling is a critical step in poultry processing to attain high-quality meat and to meet the USDA-Food Safety and Inspection Service temperature standards. This study was conducted to determine the effects of commercially available chilling systems on quality and safety of broiler meat. A total of 300 carcasses in 2 replications were randomly selected from a commercial processor and subjected to 3 systems: immersion chill (IC), air chill (AC), and combi in-line air chill (CIAC). Incidence of Salmonella and Campylobacter were determined on pre- and postchilled carcasses. Quality of the meat was evaluated by carcass yield, drip loss, cook loss, texture, moisture content, sensory qualities, and color (L*, a*, and b*) of boneless skinless breast fillets and skin-on drums. Shelf life of whole carcasses, breast fillets, and drums was also determined. The IC resulted in the most reduction of Salmonella (39.7%) and Campylobacter (43%) incidence due to the washing effect and presence of chlorine in the chilled water. There was no significant difference in shelf-life when comparing the chilling methods. The IC had the highest (P < 0.05) carcass yield (6.5%), followed by CIAC (+1.98.0%) and then AC (-1.10%). Drip loss, cook loss, and moisture content of breast fillets were not significantly different for all the chilling systems, but higher L* value was observed for breast fillets at 24 h postmortem treated with IC and CIAC. However, IC exhibited the lightest color and AC was darkest in the drum samples. Shear force of breast meat was significantly more tender for AC and CIAC. There were no differences in the sensory qualities of breast fillets and drums among the 3 chilling systems.
- Published
- 2013
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18. Attachment of Salmonella serovars and Listeria monocytogenes to stainless steel and plastic conveyor belts.
- Author
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Veluz GA, Pitchiah S, and Alvarado CZ
- Subjects
- Abattoirs, Animals, Polypropylenes, Polyurethanes, Poultry, Surface Properties, Bacterial Adhesion physiology, Listeria monocytogenes physiology, Plastics, Salmonella physiology, Stainless Steel
- Abstract
In poultry industry, cross-contamination due to processing equipment and contact surfaces is very common. This study examined the extent of bacterial attachment to 6 different types and design of conveyor belts: stainless steel-single loop, stainless steel-balance weave, polyurethane with mono-polyester fabric, acetal, polypropylene mesh top, and polypropylene. Clean conveyor belts were immersed separately in either a cocktail of Salmonella serovars (Salmonella Typhimurium and Salmonella Enteritidis) or Listeria monocytogenes strains (Scott A, Brie 1, ATCC 6744) for 1 h at room temperature. Soiled conveyor chips were dipped in poultry rinses contaminated with Salmonella or Listeria cocktail and incubated at 10°C for 48 h. The polyurethane with mono-polyester fabric conveyor belt and chip exhibited a higher (P<0.05) mean number of attached Salmonella serovars (clean: 1.6 to 3.6 cfu/cm2; soiled: 0.8 to 2.4 cfu/cm2) and L. monocytogenes (clean: 4.0 to 4.3 cfu/cm2; soiled: 0.3 to 2.1 cfu/cm2) in both clean and soiled conditions. The stainless steel conveyor belt attached a lower (P<0.05) number of Salmonella serovars (clean: 0 to 2.6 cfu/cm2; soiled: 0.4 to 1.3 cfu/cm2) and L. monocytogenes (clean: 0.4 to 2.9 cfu/cm2; soiled: 0 to 0.7 cfu/cm2) than the polymeric materials, indicating weaker adhesion properties. Plastic conveyor belts exhibited stronger bacterial adhesion compared with stainless steel. The result suggests the importance of selecting the design and finishes of conveyor belt materials that are most resistant to bacterial attachment.
- Published
- 2012
- Full Text
- View/download PDF
19. Postrigor tumble marination strategies for improving color and water-holding capacity in normal and pale broiler breast fillets.
- Author
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Gorsuch V and Alvarado CZ
- Subjects
- Animals, Chickens, Cooking, Humans, Meat standards, Phosphates, Sodium Chloride, Taste, Water analysis, Food Handling methods, Muscle, Skeletal chemistry
- Abstract
Pale or pale, soft, and exudative-like meat can be caused by a decline in pH early postmortem while carcass temperatures are still high. This decrease in pH leads to protein denaturation, attributing to the pale color and poor water-holding capacity that is characteristic of this lesser quality meat. Marination with NaCl and phosphates has been shown to improve protein functionality, thereby reducing lost meat yield and improving meat quality. However, there are few studies relating marination with phosphates to improvements in pale meat. Therefore, the purpose of this experiment was to determine if meat quality improvements could be obtained in pale meat via marination with various phosphate and NaCl treatments without altering the quality and stability of normal or pale meat. The treatments used in this study were 1) sodium tripolyphosphate, an industry control; 2) a high pH phosphate (11.9); 3) a sodium tripolyphosphate and high pH mixture; 4) an agglomerated phosphate; and 5) a nonagglomerated phosphate. The marinades used in this study increased the pH, decreased the L* values of the pale fillets, and improved water-holding capacity. There were no significant differences in overall flavor preference for any of the 5 phosphate treatments. There was also no difference in oxidation or shelf-life trends in either the pale or normal fillets marinated with each of the 5 treatments. The results of this study were that marination with phosphates can be used to marinate pale meat without altering flavor, increasing the development of oxidation, or reducing shelf life.
- Published
- 2010
- Full Text
- View/download PDF
20. Validation of a lactic acid- and citric acid-based antimicrobial product for the reduction of Escherichia coli O157: H7 and Salmonella on beef tips and whole chicken carcasses.
- Author
-
Laury AM, Alvarado MV, Nace G, Alvarado CZ, Brooks JC, Echeverry A, and Brashears MM
- Subjects
- Animals, Cattle, Citric Acid pharmacology, Colony Count, Microbial, Consumer Product Safety, Dose-Response Relationship, Drug, Food Contamination analysis, Food Contamination prevention & control, Humans, Lactic Acid pharmacology, Time Factors, Anti-Bacterial Agents pharmacology, Chickens microbiology, Escherichia coli O157 drug effects, Food Handling methods, Meat microbiology, Salmonella drug effects
- Abstract
The objectives of this study were to determine the effects of a lactic acid- and citric acid-based antimicrobial product on the reduction of Salmonella on whole broiler carcasses during processing and the reduction of Salmonella and Escherichia coli O157:H7 on beef trim. Freshly harvested broiler carcasses were inoculated with an inoculum of Salmonella strains to yield a 10(5) CFU/ml pathogen load on the surface of the carcass. The beef tips were inoculated as well with an inoculum of either E. coli O157:H7 or Salmonella to yield 10(4) CFU/100 cm(2). After 30 min for attachment, the broiler carcasses were treated with Chicxide applied for 5 s via a spray or immersed in Chicxide for 5, 10, or 20 s. Broiler carcasses were rinsed in poultry rinse bags with 400 ml of Butterfield's phosphate buffer in which Salmonella was enumerated from the diluents and Butterfield's phosphate. Chicxide significantly reduced Salmonella by 1.3 log CFU/ml with spray treatment and 2.3 log CFU/ml for all dip treatments. Following 30 min of attachment, the beef tips were placed into a spray cabinet with either Beefxide or sterilized water (control) and sprayed at 1 ft/2.5 s chain speed at 40 lb/in(2). The external surface of each beef tip was swabbed (100 cm(2)) to determine pathogen loads. Beefxide significantly reduced E. coli O157:H7 by 1.4 log CFU/100 cm(2) and Salmonella by 1.1 log CFU/100 cm(2) (P < 0.05) compared with the control samples.
- Published
- 2009
- Full Text
- View/download PDF
21. Control of Listeria monocytogenes in turkey deli loaves using organic acids as formulation ingredients.
- Author
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Lloyd T, Alvarado CZ, Brashears MM, Thompson LD, McKee SR, and Berrang M
- Subjects
- Animals, Colony Count, Microbial, Turkeys, Acetates pharmacology, Food Microbiology, Lactates pharmacology, Listeria monocytogenes growth & development, Listeriosis prevention & control, Meat Products microbiology
- Abstract
The growth of Listeria monocytogenes in further-processed meat products has become a major concern and an important food safety issue. The meat and poultry industries have incorporated interventions such as organic acids in marinades to inhibit the growth of L. monocytogenes. In this study, organic acids were utilized in the raw product and as a postcook dip to determine their inhibitory effect on the growth of L. monocytogenes in turkey deli loaves. The turkey deli loaves were processed, cooked, cooled, inoculated with streptomycin-resistant L. monocytogenes, and then dipped. Treatments were potassium lactate (PL) in the raw product with sodium lactate (SL), sodium diacetate (SD) dip, PL with SL/PL/SD dip, SL with SL/SD dip, and SL with SL/PL/SD dip. There was also a positive (inoculated) and negative (noninoculated) control, which was dipped in distilled water. Days 0, 7, 14, 21, 28, 42, and 56 were sampled for L. monocytogenes. There were no differences (P>0.05) among the organic acid treatments in the turkey deli loaves at any time points; therefore, all of the treatments increased the lag phase of L. monocytogenes, extending the shelf-life of the product. However, there was a difference between the treatments and the positive control at d 7, 14, 21, 28, 42, and 56. The growth of L. monocytogenes increased immediately in the positive control, whereas the negative control appeared to have no growth. These organic acids can provide meat processors with a useful method for extending the lag phase of L. monocytogenes in ready-to-eat meat and poultry products.
- Published
- 2009
- Full Text
- View/download PDF
22. Research developments in pale, soft, and exudative turkey meat in North America.
- Author
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Owens CM, Alvarado CZ, and Sams AR
- Subjects
- Animals, Food Handling methods, North America, Research, Stress, Physiological genetics, Turkeys genetics, Meat standards
- Abstract
Pale, soft, and exudative (PSE) refers to meat that is pale in color, forms soft gels, and has poor water-holding ability. Most frequently used in reference to pork, this defective meat is being seen with increasing frequency in turkey and broiler processing plants. It has been estimated that this PSE-type meat represents 5 to 40% of meat that is produced in the poultry industry. With the increased production of further-processed products, this PSE problem has become more apparent in the turkey industry. It has been estimated that due to the high incidence, a single turkey processing plant could be losing $2 to 4 million per year, resulting in a loss in excess of $200 million dollars by the turkey industry alone.
- Published
- 2009
- Full Text
- View/download PDF
23. Comparison of quality attributes of shell eggs subjected to directional microwave technology.
- Author
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Lakins DG, Alvarado CZ, Luna AM, O'Keefe SF, Boyce JB, Thompson LD, Brashears MT, Brooks JC, and Brashears MM
- Subjects
- Color, Consumer Behavior, Egg Proteins chemistry, Egg Yolk chemistry, Female, Food-Processing Industry methods, Humans, Hydrogen-Ion Concentration, Male, Vitelline Membrane physiology, Water chemistry, Eggs standards, Food Handling methods, Microwaves
- Abstract
Microwaves have been shown to cause thermal as well as nonthermal destruction of pathogens such as Salmonella, which can be found in shell eggs. The objective of this study was to determine if using microwave technology would cause detrimental quality effects in shell eggs. Treatments included control (no treatment) and microwave-treated (20 s) shell eggs. There were no differences in mineral content, fatty acid profile, Haugh units, broken-out score, yolk index, emulsion stability, pH of whole egg, and foaming capacity between 2 treatments (P >or= 0.05). At 0 and 30 d, there were no noticeable differences in H(2)O activity between 2 treatments. The foaming stability and albumen thermocoagulation of microwave-treated eggs were significantly higher than control eggs (P
or= 0.05). Therefore, microwave technology can be applied to shell eggs without causing detrimental effects to quality. - Published
- 2009
- Full Text
- View/download PDF
24. Reduction of Salmonella Enteritidis in shell eggs using directional microwave technology.
- Author
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Lakins DG, Alvarado CZ, Thompson LD, Brashears MT, Brooks JC, and Brashears MM
- Subjects
- Animals, Chickens, Hydrogen-Ion Concentration radiation effects, Ovum chemistry, Ovum radiation effects, Food Handling methods, Microwaves, Ovum microbiology, Salmonella enteritidis radiation effects
- Abstract
Microwaves have been shown to cause thermal as well as nonthermal destruction of pathogens such as Salmonella Enteritidis, which is commonly found in shell eggs. The objective of this study was to examine the use of new directional microwave technology to reduce Salmonella Enteritidis without causing any detrimental effects on quality in white and brown eggs. Treatments were control and microwaved white and brown eggs. Applying directional microwave technology resulted in a 2-log reduction of Salmonella Enteritidis in both the high (10(5) cfu/g) and low (10(2) cfu/g) inoculum. At d 0, there were no differences in water activities, albumen pH, and combined pH between treatments; however, there were significant changes in yolk pH. Collectively, these results indicate that applying directional microwave technology can reduce Salmonella Enteritidis in shell eggs without causing any detrimental effects to quality.
- Published
- 2008
- Full Text
- View/download PDF
25. Quality of and mold growth on white enriched bread for military rations following directional microwave treatment.
- Author
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Lakins DG, Echeverry A, Alvarado CZ, Brooks JC, Brashears MT, and Brashears MM
- Subjects
- Colony Count, Microbial, Consumer Behavior, Consumer Product Safety, Food Handling methods, Food, Fortified, Humans, Taste, Temperature, Time Factors, Water metabolism, Bread microbiology, Food Preservation methods, Fungi growth & development, Fungi radiation effects, Microwaves
- Abstract
Meals ready-to-eat (MRE) are self-contained and flexible packages used by military personnel while in the field to store food for an extended period of time; however, inclusion of white bread is not a common practice because of short shelf life stability and spoilage. The objective of this study was to determine mold inhibition and quality attributes over a 60-d period after applying directional microwaves. Different bread loaves were used for quality and for microbiological experiments. For microbiological analysis, bread was exposed to 0-, 5-, 6-, 7-, 8-, 9-, and 10-s directional microwave treatments after inoculation with a 3 strain cocktail of common bread mold, stored at 25 degrees C for 60 d, and monitored for mold growth. For quality analysis, bread was exposed to 0- and 10-s treatments, stored at 25 degrees C, and moisture, water activity (a(w)), softness, and sensory analysis were analyzed on 0, 7, 14, 28, 45, and 60 d. There was no quantifiable mold present at day 0 when treated for 10 s (P < 0.05). By day 60, the 10-s treatment had significantly lower counts (< 3 CFU/g) than the remaining treatments. Directional microwave treatment significantly decreased the moisture content of the bread but was not detectable by consumers. There was no difference in a(w) through day 45 but differences were detected at day 60. There were no differences in softness (mm) of the treated and untreated bread through day 60. No differences were detected by sensory analysis. Directional microwaves can be used to extend the shelf life of white enriched bread up 2 mo with minimal mold growth and without detrimental effect to quality.
- Published
- 2008
- Full Text
- View/download PDF
26. Use of UV light for the inactivation of Listeria monocytogenes and lactic acid bacteria species in recirculated chill brines.
- Author
-
Gailunas KM, Matak KE, Boyer RR, Alvarado CZ, Williams RC, and Sumner SS
- Subjects
- Animals, Colony Count, Microbial, Consumer Product Safety, Food Contamination analysis, Food Contamination prevention & control, Food Handling methods, Food Microbiology, Hygiene, Ultraviolet Rays, Food Irradiation methods, Lactobacillus radiation effects, Listeria monocytogenes radiation effects, Meat Products microbiology, Salts
- Abstract
Ready-to-eat meat products have been implicated in several foodborne listeriosis outbreaks. Microbial contamination of these products can occur after thermal processing when products are chilled in salt brines. The objective of this study was to evaluate UV radiation on the inactivation of Listeria monocytogenes and lactic acid bacteria in a model brine chiller system. Two concentrations of brine (7.9% [wt/wt] or 13.2% [wt/wt]) were inoculated with a approximately 6.0 log CFU/ml cocktail of L. monocytogenes or lactic acid bacteria and passed through a UV treatment system for 60 min. Three replications of each bacteria-and-brine combination were performed and resulted in at least a 4.5-log reduction in microbial numbers in all treated brines after exposure to UV light. Bacterial populations were significantly reduced after 5 min of exposure to UV light in the model brine chiller compared with the control, which received no UV light exposure (P < 0.05). The maximum rate of inactivation for both microorganisms in treated brines occurred between minutes 1 and 15 of UV exposure. Results indicate that in-line treatment of chill brines with UV light reduces the number of L. monocytogenes and lactic acid bacteria.
- Published
- 2008
- Full Text
- View/download PDF
27. Comparison of air and immersion chilling on meat quality and shelf life of marinated broiler breast fillets.
- Author
-
Carroll CD and Alvarado CZ
- Subjects
- Animals, Chickens, Cooking, Food Preservation methods, Meat standards, Refrigeration
- Abstract
Marinated broiler breast fillets were evaluated using both air- and immersion-chilling treatments. Ninety fillets from air-chilled broiler carcasses and 90 fillets from immersion-chilled broiler carcasses were obtained from a processor to determine differences in meat quality, sensory, and shelf life. At 24 h postmortem, the fillets were vacuum-tumbled (25 in Hg, 30 min, 14 rpm, 4 degrees C) in 2 replications per treatment with a 20% solution (wt/wt) yielding 0.70% NaCl and 0.45% sodium tripolyphosphate in the final product. One-third of the fillets in each replication were packaged in a tray covered with plastic wrap and stored in retail cases to simulate retail shelf-life conditions. The remaining fillets were stored at 4 degrees C for 24 h until analysis for marinade retention, cook loss, consumer evaluation, and objective tenderness. The immersion-chilled fillets had a significantly lower pH (5.56) and were lighter (L* 54.73) when compared with the air-chilled fillets (5.64, L* 50.13, respectively). The air-chilled fillets had a significantly higher marinade pickup (15.51%) than the immersion-chilled (14.07%) fillets. However, there were no significant differences in cook loss percentage in either treatment (approximately 20.03). Shear value was significantly higher in the immersion-chilled fillets (4.14 N), indicating less tender meat than the air-chilled fillets (3.62 N). In the consumer analyses, the air-chilled fillets were significantly different. Of the respondents that noted differences, 19% indicated differences in texture, and 9.67% indicated taste differences. The air-chilled treatment had significantly lower aerobic plate count in postpackaging d 0, 3, and 9. Also, coliforms were significantly lower in the air-chilled treatment through d 6. Therefore, air chilling carcasses may improve color, marination yield, tenderness, and increase the shelf life of retail-packaged broiler breast fillets.
- Published
- 2008
- Full Text
- View/download PDF
28. Marination of turkey breast fillets to control the growth of Listeria monocytogenes and improve meat quality in deli loaves.
- Author
-
Carroll CD, Alvarado CZ, Brashears MM, Thompson LD, and Boyce J
- Subjects
- Animals, Time Factors, Food Microbiology standards, Food Preservation methods, Food Preservatives pharmacology, Listeria monocytogenes drug effects, Meat microbiology, Meat standards, Turkeys microbiology
- Abstract
Turkey deli loaves were evaluated using organic marinades in the raw product to control the growth of Listeria monocytogenes (LM) and improve meat quality in the cooked product. Treatments included sodium tripolyphosphate (STP; 0.45%, control), sodium lactate (3%), sodium diacetate (0.25%), sodium citrate (0.75%), and sodium lactate (3%)/sodium diacetate (0.25%) combination, all containing 1.5% salt. Data collected in the 2 trials included pH; lightness, redness, and yellowness; bind ability; cooked meat moisture; oxidation (thiobarbituric acid reactive substances); aerobic plate count (d 0 to 80); and sensory evaluation. Also, thirty-two slices from each loaf were inoculated with a 10(3) cfu/ mL surface inoculum streptomycin-resistant LM cocktail and analyzed for LM levels (d 0 to 77). The sodium lactate treatment was lower in pH (5.84) postmarination. Lactate, citrate, and the combination treatments had significantly lower lightness values; lactate, diacetate, and citrate had higher redness values; and lactate had lower yellowness values postmarination compared with premarination. Cook loss, moisture, and bind ability were higher in the STP treatment. Citrate and the combination treatments had lower thiobarbituric acid reactive substances on d 3, but there were no differences by d 15. Cohesiveness was significantly higher in the STP, lactate, and diacetate treatments, and turkey flavor was more intense in the combination treatment. The STP loaves had >10(6) cfu/cm(2) aerobic plate count by 14 d, lactate by 20 d, citrate by 40 d, diacetate by 70 d, and lactate/diacetate by 74 d. Sodium diacetate, citrate, and lactate/diacetate all extended the lag phase of LM. Therefore, acidic marinades applied in the raw product do have a negative effect on some cooked product quality attributes but do improve shelf life and decrease LM growth by extending the lag phase through 21 d postmortem.
- Published
- 2007
- Full Text
- View/download PDF
29. The effect of blood removal on oxidation and shelf life of broiler breast meat.
- Author
-
Alvarado CZ, Richards MP, O'Keefe SF, and Wang H
- Subjects
- Abattoirs, Animals, Chickens, Color, Electroshock, Hemoglobins analysis, Hydrogen-Ion Concentration, Hypoxia, Lipid Metabolism, Meat analysis, Oxidation-Reduction, Postmortem Changes, Time Factors, Food Handling methods, Food Preservation methods, Meat standards
- Abstract
Blood components, especially hemoglobin, are powerful promoters of lipid oxidation and may decrease the shelf life of meat products. Therefore, this study examined different slaughter techniques to determine their effects on pH (24 h), color (L*a*b* values at 24 h), lipid oxidation, residual hemoglobin concentration (24 h), and sensory evaluation (d 1 and 4 postmortem; PM) in broiler breast fillets. The treatments included 1) CO(2) slaughter and not bled, 2) no stunning and bled, 3) electrical stunning (ES) and bled, 4) CO(2) stunning and bled, and 5) ES and decapitation. The birds were conventionally processed, and analyses were performed at 24 h PM except residual hemoglobin for which the samples were frozen (-80 degrees C) until analyses ( < 2 mo). There were no significant differences in pH or b* values at 24 h PM among any of the treatments. L* values were significantly higher, indicating lighter fillets in the ES and decapitated birds compared with the darker fillets from the CO(2) stunned and bled birds. The CO(2) slaughter and not bled birds had significantly higher a* values, indicating more red color, when compared with the ES and bled and decapitated birds. There were no significant differences in the residual hemoglobin contents in the broiler breast muscle when comparing all of the treatments except CO(2) slaughter and not bled, which was significantly (around 15%) greater. Overall TBA-reactive substances (TBARS; raw, cooked at 24 h, and cooked at 72 h PM) indicated that ES and bled birds had the lowest TBARS when compared with the remaining treatments. Consumer panels detected increased aroma (chicken meaty and warmed-over aromas) and flavor (chicken meaty and warmed-over flavors) in not bled samples at 24 h PM. By 72 h PM, however, there were no significant differences in aroma or flavor. Therefore, different slaughter and bleeding method may affect color and sensory properties of the broiler breast fillets, and the ES and decapitation method had the most favorable results for sensory quality.
- Published
- 2007
- Full Text
- View/download PDF
30. Planning our future: the Poultry Science Association strategic plan.
- Author
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Hester PY, Alvarado CZ, Bilgili SF, Denton JH, Donoghue AM, Giesen A, Hargis BM, Kessler JW, Madison FN, Malone GW, Mavrolas P, Noll SL, Pescatore AJ, Ricks CA, Robinson FE, Shirley RB, Sifri M, Smith MO, Stonerock RH, Wilson JL, Wineland MJ, Beck MM, and Saylor WW
- Subjects
- Animals, Goals, Organizational Objectives, United States, Poultry, Societies, Scientific organization & administration, Societies, Scientific trends
- Published
- 2005
- Full Text
- View/download PDF
31. Turkey carcass chilling and protein denaturation in the development of pale, soft, and exudative meat.
- Author
-
Alvarado CZ and Sams AR
- Subjects
- Animals, Food Handling methods, Hydrogen-Ion Concentration, Cold Temperature, Food Technology, Meat, Muscle Proteins chemistry, Protein Denaturation, Turkeys
- Abstract
Pale, soft, and exudative (PSE) meat is a growing problem in the turkey industry and has been associated with processing conditions such as slow carcass chilling. The development of PSE meat is caused by protein denaturation resulting from a rapid rate of pH decline early postmortem (PM) while carcass temperatures are still elevated. This research was conducted to determine the relationship of slow chilling to protein denaturation and PSE development. A total of 48 toms were conventionally processed in 2 trials at 22.5 wk of age, and chilled at 0, 10, 20, or 30 degrees C for either 45 or 90 min before deboning (at 60 or 105 min PM). Temperature and pH of the breast muscle was recorded at 15 min PM, at the time of deboning (60 or 90 min PM), and at 24 h PM. Color was determined at deboning and again at 24 h PM. Gel strength, cook loss, expressible moisture, total protein solubility, and bound phosphorylase quantities were determined on the fillets at 24 h PM. There was no difference in carcass temperature at 15 min PM, but by 105 min PM each temperature treatment was significantly different, with the carcasses chilled at 0 and 10 degrees C having the lowest temperature, the 30 degrees C-chilled birds having the highest temperature, and the 20 degrees C-chilled carcasses being intermediate but significantly different from either extreme. The carcass temperature differences at 105 min PM indicated that the carcass experienced differing chilling rates. To varying degrees, slower rates of chilling resulted in lower pH, greater degree of lightness (L* value), greater cook loss, and reduced gel strength. However, chilling rate had no effect on total protein solubility or myofibrillar phosphorylase for any of the treatments. Chilling rate seems to contribute to PSE turkey meat characteristics but by a mechanism independent of total protein solubility or myofibrillar phosphorylase.
- Published
- 2004
- Full Text
- View/download PDF
32. Early postmortem injection and tumble marination effects on broiler breast meat tenderness.
- Author
-
Alvarado CZ and Sams AR
- Subjects
- Animals, Chemical Phenomena, Chemistry, Physical, Food Technology, Muscle Contraction, Muscle, Skeletal ultrastructure, Rheology, Sarcomeres ultrastructure, Chickens, Food Handling methods, Meat
- Abstract
Several processors use inline injection and tumble marination practices to decrease labor costs and product handling. However, the stimulation of this early postmortem (PM) muscle may cause increased toughness. Therefore, the objective of this study was to compare marination of early PM meat by using injection, vacuum tumbling, or both to determine their effects on tenderness of broiler breast fillets. Breast fillets from 45 broilers were deboned at 3 h PM and injected, tumbled (30 min, 635 mmHg, 14 rpm), or injected + tumbled with a 10% solution of 0.54%. NaCl + 0.42% sodium tripoly phosphate (STPP). Nonmarinated controls deboned at 3 and 6 h PM were also included. Shear value, sarcomere length, and cook loss were determined on each of the fillets. The control and tumbled fillets had the highest shear value compared with the remaining 3-h treatments. The injected + tumbled and tumbled fillets had the highest cook loss followed by 3-h control and injected treatments. Tumble marination at 3 h PM produced significantly tougher meat then the injection treatment. Experiment 2 was conducted to test marinade penetration through the fillets because penetration could have affected the results of the previous study. Broiler breast fillets were deboned from 60 broilers at 3 h PM and were vacuum tumbled (30 min, 635 mmHg, 14 rpm) with a 15% solution of 0.54% NaCl and 0.42% STPP. We determined shear value and cooked meat moisture of the fillets and sodium ion migration into the fillets. Although there were no significant differences in shear value between control and tumbled fillets, the cooked meat moisture was significantly higher in tumbled fillets when compared with the controls. Sodium ion concentration was significantly higher on the surface (especially the lateral surface) of the fillets when compared with the center, which suggested some uneven distribution.
- Published
- 2004
- Full Text
- View/download PDF
33. Utilization of response surface modeling to evaluate the effects of non-meat adjuncts and combinations of PSE and RFN pork on water holding capacity and cooked color in the production of boneless cured pork.
- Author
-
Schilling MW, Marriott NG, Acton JC, Anderson-Cook C, Alvarado CZ, and Wang H
- Abstract
Boneless cured pork was produced from combinations of pale, soft, and exudative (PSE) and red, firm, and non-exudative (RFN) semimembranosus muscle. Response Surface Methodology was utilized to determine the effects of soy protein concentrate (SPC), sodium caseinate (SC), and modified food starch (MFS) on the water holding capacity and cooked color in a chunked and formed product. Fifteen ingredient combinations were replicated three times for each PSE and RFN combination giving 75 treatments per replication. Utilization of SPC decreased (P<0.01) cooking loss and redness while increasing (P<0.01) yellowness. MFS decreased (P<0.01) expressible moisture, and both MFS and SC increased (P<0.05) cooked redness while decreasing (P<0.01) cooked lightness. Product formulations using these adjuncts demonstrate potential to improve the water-holding capacity and cooked color in PSE as well as RFN pork. This research also demonstrated that diluting RFN pork with no more than 25% PSE pork permits the formation of a high quality boneless deli ham roll.
- Published
- 2004
- Full Text
- View/download PDF
34. Utilization of pork collagen for functionality improvement of boneless cured ham manufactured from pale, soft, and exudative pork.
- Author
-
Schilling MW, Mink LE, Gochenour PS, Marriott NG, and Alvarado CZ
- Abstract
This study was designed to determine the effect of raw material and the inclusion of pork collagen on the protein functionality of boneless cured pork manufactured from 100% pale, soft, and exudative (PSE), 50% PSE, and 0% PSE with either 3 or 0% collagen. A Randomized Complete Block Design with six replications was utilized as the experimental design. Inclusion of collagen decreased (P<0.05) expressible moisture and increased (P<0.05) CIE b* value. Differences (P<0.05) revealed that collagen inclusion caused the 0% PSE treatments to have a lower cooking loss for 100% PSE treatments with and without collagen and a higher protein-protein bind value for 100% PSE treatments without collagen. Utilization of pork collagen in boneless cured pork that incorporates PSE meat increases water holding capacity and has the potential to improve protein functionality characteristics of the product.
- Published
- 2003
- Full Text
- View/download PDF
35. Rigor mortis development in turkey breast muscle and the effect of electrical stunning.
- Author
-
Alvarado CZ and Sams AR
- Subjects
- Animals, Muscle, Skeletal ultrastructure, Postmortem Changes, Rigor Mortis, Sarcomeres physiology, Sarcomeres ultrastructure, Turkeys, Abattoirs, Electroshock, Meat, Muscle, Skeletal physiology
- Abstract
Rigor mortis development in turkey breast muscle and the effect of electrical stunning on this process are not well characterized. Some electrical stunning procedures have been known to inhibit postmortem (PM) biochemical reactions, thereby delaying the onset of rigor mortis in broilers. Therefore, this study was designed to characterize rigor mortis development in stunned and unstunned turkeys. A total of 154 turkey toms in two trials were conventionally processed at 20 to 22 wk of age. Turkeys were either stunned with a pulsed direct current (500 Hz, 50% duty cycle) at 35 mA (40 V) in a saline bath for 12 seconds or left unstunned as controls. At 15 min and 1, 2, 4, 8, 12, and 24 h PM, pectoralis samples were collected to determine pH, R-value, L* value, sarcomere length, and shear value. In Trial 1, the samples obtained for pH, R-value, and sarcomere length were divided into surface and interior samples. There were no significant differences between the surface and interior samples among any parameters measured. Muscle pH significantly decreased over time in stunned and unstunned birds through 2 h PM. The R-values increased to 8 h PM in unstunned birds and 24 h PM in stunned birds. The L* values increased over time, with no significant differences after 1 h PM for the controls and 2 h PM for the stunned birds. Sarcomere length increased through 2 h PM in the controls and 12 h PM in the stunned fillets. Cooked meat shear values decreased through the 1 h PM deboning time in the control fillets and 2 h PM in the stunned fillets. These results suggest that stunning delayed the development of rigor mortis through 2 h PM, but had no significant effect on the measured parameters at later time points, and that deboning turkey breasts at 2 h PM or later will not significantly impair meat tenderness.
- Published
- 2000
- Full Text
- View/download PDF
36. The influence of postmortem electrical stimulation on rigor mortis development, calpastatin activity, and tenderness in broiler and duck pectoralis.
- Author
-
Alvarado CZ and Sams AR
- Subjects
- Animals, Chickens, Ducks, Hydrogen-Ion Concentration, Lactic Acid metabolism, Muscle Fibers, Fast-Twitch physiology, Muscle, Skeletal physiology, Calcium-Binding Proteins metabolism, Electric Stimulation, Food Technology, Postmortem Changes, Poultry, Rigor Mortis
- Abstract
This study was conducted to evaluate the effects of electrical stimulation (ES) on rigor mortis development, calpastatin activity, and tenderness in anatomically similar avian muscles composed primarily of either red or white muscle fibers. A total of 72 broilers and 72 White Pekin ducks were either treated with postmortem (PM) ES (450 mA) at the neck in a 1% NaCl solution for 2 s on and 1 s off for a total of 15 s or were used as nonstimulated controls. Both pectoralis muscles were harvested from the carcasses after 0.25, 1.25, and 24 h PM and analyzed for pH, inosine:adenosine ratio (R-value), sarcomere length, gravimetric fragmentation index, calpastatin activity, shear value, and cook loss. All data were analyzed within species for the effects of ES. Electrically stimulated ducks had a lower muscle pH at 0.25 and 1.25 h PM and higher R-values at 0.25 h PM compared with controls. Electrically stimulated broilers had a lower muscle pH at 1.25 h and higher R-values at 0.25 and 1.25 h PM compared with controls. Muscles of electrically stimulated broilers exhibited increased myofibrillar fragmentation at 0.25 and 1.25 h PM, whereas there was no such difference over PM time in the duck muscle. Electrical stimulation did not affect calpastatin activity in either broilers or ducks; however, the calpastatin activity of the broilers did decrease over the aging time period, whereas that of the ducks did not. Electrical stimulation decreased shear values in broilers at 1.25 h PM compared with controls; however, there was no difference in shear values of duck muscle due to ES at any sampling time. Cook loss was lower for electrically stimulated broilers at 0.25 and 1.25 h PM compared with the controls, but had no effect in the ducks. These results suggest that the red fibers of the duck pectoralis have less potential for rigor mortis acceleration and tenderization due to ES than do the white fibers of the broiler pectoralis.
- Published
- 2000
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