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Alum is the most used vaccine adjuvant, due to its safety, low cost and adjuvanticity to various antigens. However, the mechanism of action of alum is complex and not yet fully understood, and the immune responses elicited can be weak and antigen-dependent. While several antigens rapidly desorb from alum upon exposure to serum, phosphorylated proteins remain tightly bound through a ligand-exchange reaction with surface hydroxyls on alum. Here, bacterial proteins and glycoconjugates have been modified with phosphoserines, aiming at enhancing the binding to alum and prolonging their bioavailability. Tetanus toxoid protein and Salmonella Typhi fragmented Vi-CRM conjugate were used. Both antigens rapidly and completely desorbed from alum after incubation with serum, verified via a competitive ELISA assay, and set up to rapidly evaluate in vitro antigen desorption from alum. After antigen modification with phosphoserines, desorption from alum was slowed down, and modified antigens demonstrated more prolonged retention at the injection sites through in vivo optical imaging in mice. Both modified antigens elicited stronger immune responses in mice, after a single injection only, compared to unmodified antigens. A stronger binding to alum could result in potent single-dose vaccine candidates and opens the possibility to design novel carrier proteins for glycoconjugates and improved versions of bacterial recombinant proteins. [ABSTRACT FROM AUTHOR]

This study aims to analyze the cost of using lime and alum in the treatment of acid mine drainage at Settling pond 03, Pit Paku, PT. Rimau Energy Mining. Acid mine drainage, formed through chemical reactions between sulfide minerals and water, requires specialized treatment to mitigate its environmental impact. The use of lime and alum is intended to increase pH and reduce turbidity levels in the acid mine water. Through laboratory experiments with various doses of lime and alum, this research identified the most effective doses to neutralize acid mine drainage, raise pH levels, and reduce turbidity in accordance with established environmental standards. Additionally, the study analyzed the costs associated with each dose combination tested, revealing that the optimal lime and alum dose combinations resulted in more cost-effective treatment compared to previously employed methods. he effective dose to neutralize acid mine water is 1 gram of lime and 2 grams of alum per liter, which successfully increases the pH from 3.88-4.13 to 8.10-8.40, and reduces turbidity from 498-522 NTU to 144.9-196.1 NTU. [ABSTRACT FROM AUTHOR]

The spent alum sludge was employed for the removal of specific fission products including 137Cs, 85Sr, 152+154Eu, 99Mo, and 99Tc radionuclides from radioactive waste. The physicochemical features of spent alum sludge were investigated. The results illustrated that the uptake percentages were 94.2, 90.5, 87.6, 87.1, and 76.6%, correspondingly, for 99Mo, 152+154Eu, 99Tc, 85Sr, and 137Cs. The kinetic studies illustrated that the adsorption process obeyed Langmuir kinetics which is a combination of first-order and second-order expressions. The findings demonstrated that the spent alum sludge can be applied successfully as a low-cost and effective adsorbent for the treatment of radioactive waste. [ABSTRACT FROM AUTHOR]

This study explored the synthesis of potash alum from disposed aluminum cans for water purification. The effect of the labels on the cans on the yield of alum was also investigated as it is economically beneficial for industry. Single crystals of potash alum were obtained and characterized by powder X-ray diffraction (P-XRD) and Fourier transform infrared (FTIR) spectroscopy. Through a series of jar test experiments, the characterized alum samples were used to purify river water samples. Some of the parameters investigated include; pH, turbidity, total suspended solids (TSS), total dissolved solids (TDS), electrical conductivity (EC) and chemical oxygen demand (COD). The results show that the alum samples were able to reduce the turbidity by 100 % and substantially decrease the TSS, TDS and COD over the five-hour period of study. However, there were slight increases in acidity and EC which could be corrected by other methods [ABSTRACT FROM AUTHOR]

Municipal solid waste (MSW) management is a significant challenge in Jordan, particularly regarding landfill leachate (LL). Al-Ghabawi landfill, the country's largest, lacks proper leachate treatment, with the current practice being limited to evaporation in open ponds, an environmentally unacceptable method. This study dealt with the coagulation process for treating LL produced from Al-Ghabawi landfills by using alum and moringa seeds as coagulants. The results showed that the optimum pH for the coagulation process was 5.5. The optimal dosages were 800 mg/L for moringa oleifera and 400 mg/L for alum (high dose), and 80 mg/L for moringa oleifera and 40 mg/L for alum (low dose). The use of moringa oleifera and alum together yielded the best removal rates for turbidity (48.9%), total suspended solids (TSS) (41.21%), total dissolved solids (TDS) (17.9%), and Mn (73.7%). Pb and Cr, however, exhibited lower removal rates, with the best achieved by using moringa seeds and alum together (42.8% and 46.58%, respectively). Notably, moringa oleifera seeds alone as a coagulant outperformed alum in removing COD (91.57%) and BOD (85.71%), indicating that moringa oleifera is a more effective option for increasing the biodegradability of the leachate, as evidenced by the increased BOD5/COD ratios, from 0.5159 to 0.875 and 1.206 for high and low doses, respectively. These findings suggest that the coagulation process can be significantly improved by utilizing moringa oleifera seeds, a natural and sustainable coagulant, in combination with alum for the treatment of Al-Ghabawi LL. [ABSTRACT FROM AUTHOR]

Aluminium is an important metal that find application in various areas ranging from simple kitchen utensils to construction and packaging materials. Due to its versatile uses, aluminium wastes are frequently generated. The aim of this research was to recycle waste aluminium foil papers used for barbequed fish packaging into useful potash alum. The method used explored the amphoteric nature of aluminium to react with both acid and alkali to form a double salt. 1.20g of the waste aluminium foil pieces was first reacted with 25ml of 3 M KOH to form a colourless solution. Further addition of 3M H2SO4 to the solution led to the precipitation of a double salt of potash alum. The percentage yield of the synthesized potash alum obtained from 1.20g waste aluminum foil was 76%. The alum also recorded 45% water of crystallization. The efficiency of the synthesized alum in water purification was also tested. Results gave 90.81% reduction in turbidity of muddy water sample from initial turbidity of 1639 NTU to 169 NTU. Thus, the potash alum synthesized from waste aluminium foils was able to coagulate fine suspension of mud from the water, therefore effective in water purification. [ABSTRACT FROM AUTHOR]

• Alum sludge is a low-cost alternative Si and Al source for zeolite synthesis. • Recycle alum sludge for zeolite synthesis reduce water treatment's environmental impact. • Zeolite synthesis from alum sludge provides a sustainable way to waste management. • Aluminosilicate synthesis using alum sludge adds an economic/environmental value. Alum sludge (AS) is a by-product generated from drinking water treatment and produced in large amounts around the world. Its chemical composition makes this waste an emerging alternative source of silicon and aluminum for aluminosilicates or zeolite material production, which can add value to residues and contribute to the circular economy process on a global scale. In this sense, and considering the scarcity of information about AS, this review shows data collection about AS in different countries, including generation, chemical composition, and disposal information. The reuse of AS is discussed based on circular economy and the environmental gains derived from such approaches are highlighted, including the possibility of utilization with other residues (e.g., ash, bioproducts, etc). Moreover, this review shows and discusses the benefits and challenges of AS reuse in the synthesis process and how it can be a sustainable raw material for aluminosilicates and zeolite synthesis. The most common conditions (conventional or non-conventional) in zeolite synthesis from AS are mentioned and advantages, limitations and trends are discussed. The discussions and data presented can improve the AS management and reuse legislations, which certainly will collaborate with sustainable AS use and circular economy processes. [ABSTRACT FROM AUTHOR]

Jute fabric samples have been dyed with aqueous solution of annatto (A) seeds along with three other natural dyes, viz. onion (O) skin, pomegranate (P) rind and marigold (M) flower petals. Varying proportions of binary mixture of these dyes have been used to dye jute fabrics which are mordanted with myrobolan and natural alum before dyeing. The dyeing performance is evaluated by measuring the colour strength and other related parameters along with the fastness properties of the dyed jute fabrics. The dyed samples are further treated with chitosan and n-cetyl trimethyl ammonium bromide (CTAB) to improve wash fastness. The treatments improve the wash fastness by 1 grade. The treatment of dyed jute fabrics with benzotriazole improves the light fastness by 1 grade. The compatibility of annatto dye with respect to other three dyes has also been assessed by plotting two different curves, viz. ΔC* vs ΔL* and K/S vs ΔL* for progressive depth of shade for each set of binary mixtures. These results are compared with a new parameter called Colour Difference Index (CDI). The results of CDI are in tandem with the results found from the plots, implying that the three binary pairs of natural dyes are comparable in different degrees. [ABSTRACT FROM AUTHOR]

Although the removal of colloidal particles continues to be an important reason for using coagulation, currently a newer approach, the removal of organic matter to reduce the formation of disinfection byproducts (DBPs) such as trihalomethanes (THMs) are suspected carcinogens. Inefficient removal of total organic carbon (TOC) leads to the formation of carcinogenic THMs. This motivates the research efforts to find a way to reduce them in potable water. The first objective of this research is monitoring the water quality (raw and produced) for selected three water treatment plants within Giza Governorate to reveal the most polluted site (high TOC in the raw water and THMs in the treated water) in the study areas. The second objective of this study is enhancing coagulation to reduce TOC and THMs from surface water treating. In this study, two different coagulants aluminum sulfate (alum), and ferric chloride were tested under different conditions of pH and coagulant concentration to determine their effectiveness for removal of TOC, THMs and turbidity. Optimum values for the mentioned conditions were used to achieve the maximum reduction for TOC and THMs in Al-Ayat drinking water treatment plant (DWTP) which recorded the highest concentration of TOC and THMs. Ferric chloride as a coagulant achieved the maximum removal percentage % of TOC, THMs and turbidity as 79%, 82% and 94%, respectively, at ferric chloride dose 100 mg/L, while alum as a coagulant recorded the maximum removal percentage % of TOC, THMs and turbidity as 46%, 52% and 91%, respectively, at alum dose 100 mg/L. The results indicated that ferric chloride as a coagulant has the highest efficiency reduction of TOC, THMs and turbidity if compared to alum with the same dose added.

Wood specimens were dyed by boiling them in the presence of walnut shells and dye to penetrate the dyestuff into the sample. Specimens prepared from Scots pine, oak, and beech woods were boiled separately in walnut shell, water, and NaOH environment, and the penetration of the dyestuff into the samples was ensured. Sodium hydroxide solution was preferred because it facilitates the dissolution of the hemicelluloses in the wood and the dyestuffs in the walnut shell. Alum was added in other samples to reveal the mordant effect on the adhesion of dyestuffs to wood. Thermogravimetric (TG) analysis revealed that wood degradation primarily occurs due to evaporation of free water at 50 to 120 °C, followed by lignin and cellulose breakdown across a wide temperature range of 160 to 600 °C. Treatment with NaOH and NaOH+alum notably reduced the peak temperatures in differential-thermogravimetric analysis by indicating an effect on lignin. This was supported by Fourier transform infrared analysis, particularly in the disappearance of carboxyl groups at 1710 cm-¹ and significant decreases in peak intensities at 1027, 1247, 1315, and 1501 cm-¹. Based on the findings, it was concluded that the dyed parts obtained can be used in the wooden toy industry.

Background: Antibiotic resistance is one of the most serious biomedical problems that require new agents to combat bacterial pathogens. Potassium aluminium sulphate (alum) has recently drawn the attention of the scientific community as an efficient, safe and eco-friendly inorganic compound with antimicrobial activity. Methods: Ten samples of bacteria, five types of Eschericha coli (E. coli) and five types of S. aureus were isolated from patients. Eschericha coli isolated from urine sample by sterile container. Staphylococcus aureus (S. aureus) isolated from wound samples by a cotton swab. The samples were cultured on the following media (Mannitol salt agar, eosin methylene blue agar, and blood agar). Well diffusion method used to evaluate the antimicrobial activity. Alum aqueous solution with two concentrations (1% and 2%) was determined as well as amoxicillin, gentamicin, and ceftriaxone. Results: E. coli is sensitive by 50%for both concentrations of alum (1%, 2%), S. aureus is 100% resistant to a concentration of 1% of alum and 50% resistant to a concentration of 2% of alum. Combination of amoxicillin with alum for each concentration (1% and 2%) has no significant effect on the activity of amoxicillin for both E. coli and S. aureus (p= 97 and p= 0.62) respectively. Combination of ceftriaxone with alum for each concentration (1% and 2%) has no significant effect. The combination of gentamicin with alum for each concentration in E. coli has no significant effect. But for S. aureus the mean of gentamicin sensitivity was 39.50± 3.87, the mean is decreased after combination with 1% alum 41.50± 4.35 and 2% alum 40± 5.83 with significant statically differences (p= 0.02). Conclusions: The white alum effect is dose response, and greater concentration will lead to enhanced decrease on bacterial growth. This study suggests more than 1% and 2% concentrations of white alum could be used as antimicrobial agent. The effect of 1% and 2% alum combined with ceftriaxone and amoxicillin shoed no significant differences (p> 0.05). [ABSTRACT FROM AUTHOR]

Objective. To identify the most effective dose of filarial rBmALT‐2 and rWbGST alone or in combination against B. malayi infection in vitro and in vivo. Methods. Mastomys (n = 5–7/group) received intramuscular (i.m.) injection with three different doses (25, 50, and 100 μg) of rBmALT‐2 or rWbGST, either alone or in combination with alum as the adjuvant. Protective immunity was studied by in vivo and in vitro cytotoxicity assay. To evaluate the cellular immune response, splenocyte proliferation and cytokine profile were assessed. Results. Serological results revealed a substantial (p < 0.005) induction of IgG1, IgG2a, and IgG3 responses in vaccinated Mastomys. Mastomys immunized with 50 μg rBmALT‐2 + alum induced 79–81% killing against the L3 larvae challenge in vivo and in vitro ADCC assay (p < 0.005); whereas rWbGST + alum alone or in combination with rBmALT‐2 + alum induced 63–68% killing (p < 0.005) in vivo and in vitro. Antigen‐specific cytokine profiles of Mastomys vaccinated with either BmALT‐2, WbGST or a combination showed elevated IL‐10, IL‐4, and IFN‐γ levels, signifying both Th1 and Th2 immune response. Conclusions. These findings suggest that immunization of Mastomys with a 50 μg/dose of rBmALT‐2 + alum four times at a 4‐week interval demonstrated considerable protection against B. malayi infection. [ABSTRACT FROM AUTHOR]

A new modified cellulose with diaminoguanidine (Cel-Gua) synthesized for specific recovery of Cu (II), Cd (II), and Hg (II) from the alum sample. Cellulose was silanized by 3-chloropropyltrimethoxysilane and then was modified with diaminoguanidine to obtain N-donor chelating fibers. Fourier transform-infrared spectroscopy, scanning electron microscopy, X-ray diffraction, zeta potential, electrons disperse X-ray analysis, elemental analyses (C, H and N), and thermogravimetric analysis were used for characterization. Factors influencing the adsorption were thoroughly examined. Under the optimal conditions, the Cel-Gua sorbent displayed maximum adsorption capacities of 94.33, 112.10 and 95.78 mg/g for Cu (II), Cd (II), and Hg (II), respectively. The sorption process of metal ions is equipped by kinetic model PSO and Langmuir adsorption isotherm. The calculated thermodynamic variables confirmed that the adsorption of Cu (II), Cd (II) and Hg (II) by Cel-Gua sorbent is a spontaneous and exothermic process. In our study, we used the molecular operating environment software to conduct molecular docking simulations on the Cel-Gua compound. The results of the docking simulations showed that the Cel-Gua compound displayed greater potency and a stronger affinity for the Avr2 effector protein derived from Fusarium oxysporum, a fungal plant pathogen (code 5OD4). The adsorbent was stable for 7 cycles, thus allowing its safe reutilization. [ABSTRACT FROM AUTHOR]

Context: The most common dental diseases are plaque-related infections. In recent years, there has been a lot of concern about caries prevention, especially in the developing countries and lower socio-economic societies. Therefore, there is always a need to find a natural mouthwash that is economical, safe and simple to make in order to improve dental health and be used as a common home treatment. Aim: The aim of the present study was to compare the effect of chlorhexidine, alum, hi-ora and Terminalia chebula extract mouthwashes on salivary Streptococcus mutans and Lactobacilli in 8-13 year old children. Materials and Methods: The sample for the study consisted of 60 school children aged 8-13 years. Children were divided randomly into four groups with 15 children each and were asked to rinse with the prescribed mouthwash once daily for 1 month, a gap period of 2 months and again continue the mouthwashes for one month and then discontinue. Simplified Oral Hygiene Index (OHI-S) and Silness - Loe Plaque Index were recorded at baseline, 15th, 30th, 90th, 120th, and 180th day. 2 ml of unstimulated saliva collection was done at baseline, 15th, 30th,90th, 120th, and 180th day for microbial analysis of Streptococcus mutans and Lactobacillus. The data's obtained was statisticaly analyzed using Kruskal Wallis Test and Repeated measures ANOVA, applied and tabulated using the SPSS software version 25.0. Results: The results of the study indicate that there was a statistically significant reduction in S. mutans and lactobacilli count in all the four study groups. Terminalia chebula extract mouth rinse was found to be the second most significantly effective antibacterial mouth rinse next to chlorhexidine mouthwash. Conclusion: The study demonstrated that alum-containing mouthwash improved plaque inhibition. Herbal mouth rinses such as Hi-ora and Terminalia Chebula have shown promising results, and thus, they can be considered as a newer alternative. [ABSTRACT FROM AUTHOR]

In 2021, an unknown Potamogeton species was discovered in Alum Creek Lake in central Ohio. After we were unable to identify the plants as any known North American species, we expanded our search to include all pondweed species and incorporated molecular data to aid in the identification. DNA sequences were obtained for the nuclear internal transcribed spacer (ITS) region and the plastid trnT-trnF region and compared against previously reported sequences for Potamogeton species. Morphological and molecular data consistently identified the plants as P. wrightii, a species native to eastern Eurasia and Malesia and not previously reported to be naturalized in North America. [ABSTRACT FROM AUTHOR]

The Crusades, which continued in Egypt and Syria between 1095-1291, brought Egyptian alum against them, and they were interested in its trade and started to carry raw materials for dyeing European wool. Even in the years when Saladin's struggles with the Crusaders continued, Genoese merchants achieved great income by transporting Egyptian alum from Alexandria to Europe. With the discovery of Anatolian alum mines towards the middle of the thirteenth century, Italian Merchants turned their attention to mines such as Focha, Gediz and Shebinkarahisar. This process has drawn them into a series of events, from power struggles to internal turmoil. In this process, the Italians, who tried to be close to the ruling powers in Anatolia from the Byzantines to the Ottomans, also entered into rivalries among themselves over alum. In this process, they increased their income by helping the Ottomans in the Byzantine and dynastic struggles, and even managed to get their debts erased. With the discovery of European alum in the middle of the fifteenth century, new fractures were experienced. With the revenues of this precious metal, the popes tried to start a new Crusade against the Ottomans, and they used the alum revenues as a weapon. Italian and Neapolitan people, to whom the Popes transferred their rights to these mining enterprises, continued the interests and interests of the Crusades. This new processal so opened the door for the Ottomans to not benefit from an important source of income properly. [ABSTRACT FROM AUTHOR]

Wood specimens were dyed by boiling them in the presence of walnut shells and dye to penetrate the dyestuff into the sample. Specimens prepared from Scots pine, oak, and beech woods were boiled separately in walnut shell, water, and NaOH environment, and the penetration of the dyestuff into the samples was ensured. Sodium hydroxide solution was preferred because it facilitates the dissolution of the hemicelluloses in the wood and the dyestuffs in the walnut shell. Alum was added in other samples to reveal the mordant effect on the adhesion of dyestuffs to wood. Thermogravimetric (TG) analysis revealed that wood degradation primarily occurs due to evaporation of free water at 50 to 120 ℃, followed by lignin and cellulose breakdown across a wide temperature range of 160 to 600 ℃. Treatment with NaOH and NaOH+alum notably reduced the peak temperatures in differential-thermogravimetric analysis by indicating an effect on lignin. This was supported by Fourier transform infrared analysis, particularly in the disappearance of carboxyl groups at 1710 cm-1 and significant decreases in peak intensities at 1027, 1247, 1315, and 1501 cm-1. Based on the findings, it was concluded that the dyed parts obtained can be used in the wooden toy industry. [ABSTRACT FROM AUTHOR]

Broiler farming is a significant source of gaseous emissions. The aim of this study was to assess the effects of different litter additives on the emission of NH3, N2O, CO2, and CH4 during broiler housing and subsequent manure storage. The gaseous emissions from the housing facilities were evaluated during one fattening cycle in environmentally controlled rooms with three different additives applied to the litter material (10% w/w aluminum sulphate or biochar and 2.50 mg m−2 urease inhibitor), as well as a control. A storage experiment was conducted under laboratory conditions for 90 days to evaluate the influence of these three additives on gaseous losses. During broiler housing, the results indicated that NH3 emissions were reduced significantly (40–60%) by litter additives, while global warming potential (GWP) emissions were reduced significantly (31%) by Alum. The addition of Biochar (a 58% reduction) had the same significant effect as Alum (a 60% reduction) to mitigate these losses. The re-application of Urease (a 41% reduction) may be required to reach an equal or higher reduction. During storage, NH3 and GWP emissions were not significantly affected by the litter additives. During broiler housing and subsequent manure storage, NH3 emissions were reduced significantly (22–41%) by litter additives, whereas GWP emissions did not decrease significantly. Globally, it can be concluded that Biochar appears to be a good alternative to Alum due to its equal effectiveness in mitigating NH3 losses, without increasing the GWP potential in the housing and avoiding pollution swapping. [ABSTRACT FROM AUTHOR]

Numerous studies have reported anomalous ultralow frequency (ULF) electromagnetic fields preceding earthquakes. In this paper, we estimate the current intensity responsible for generating the earthquake‐related ULF fields under the assumption that the origin is a current flowing at the hypocenter and that it has the same frequency dependence for all cases. To estimate current intensity, we perform ULF electromagnetic field simulations with an absorbing boundary condition developed in this study, taking into account the conductivity distribution of the Earth's crust. We analyze 11 earthquakes, including those that occurred in Loma Prieta, Spitak, Guam, Biak, Kagoshima, Iwateken Nairiku Hokubu, Izu swarm, Jammu and Kashmir, Alum Rock, Wenchuan, and L'Aquila. Our results show that, for nine out of the 11 events, there is a positive correlation between current intensity and earthquake magnitude, suggesting that the measured ULF fields originate from seismic activity and supporting our assumptions. Key Points: A positive correlation between current intensity causing ULF emissions associated with earthquakes and earthquake magnitude is indicatedThe ULF emissions are successfully computed by using the weighted Laguerre polynomial‐finite difference time domain method [ABSTRACT FROM AUTHOR]

This study aimed to optimize the removal of tannic acid (TA) from simulated wastewater by applying the electrocoagulation (EC) process and the alum nanoparticles (Al NPs) generated during the EC process, using the response surface method (RSM) methodology based on the central composite design (CCD). The structure and state of Al NPs were studied by X-ray diffraction analysis, field emission scanning electron microscopy, X-ray fluorescence (XRF), and Brunauer–Emmett–Teller analyses. We used four independent factors: current–voltage (20.83, 41.66, 62.49, 83.32, and 104.16 A/m2)/Al NPs dosage (0.1, 0.325, 0.55, 0.775, and 1 g/L), initial pH (5, 6, 7, 8, and 9/ 3, 5, 7, 9, and 11), initial TA concentration (10, 35, 60, 85, and 110 mg/L), and treatment time (10, 20, 30, 40, and 50 min). In addition, the total TA removal percentage was taken as a response variable. The analysis of the responses was performed using analysis of variance (ANOVA) as a statistical method. The quadratic model was proposed, and the ANOVA and model fitness were produced through RSM, with the coefficient of determination (R2) above 0.94 and 0.96 for all two responses representing the high importance of the suggested model. The EC process and the Al NPs removed TA with a maximum efficiency of 89.79% and 96.33%, respectively, in the optimal condition. The results indicated that the EC process and the Al NPs were effective and readily available methods for TA removal from aquatic environments. [ABSTRACT FROM AUTHOR]

Turbidity is of great concern in drinking water treatment. Coagulation-flocculation (CF) process is widely applied for removing turbidity, which depends on coagulant type. Alumchemical coagulant is commonly used due to its superiority in removing it. However, alum has been associated with several adverse impacts on human health and the environment. Natural coagulants (eco-friendly materials) cannot be a feasible alternative to alum due to their limited coagulating performance in turbidity removal. In our previous research, novel approach to reduce alum impact by partially replacing it with natural coagulant was developed based on two consecutive treatments of CF process. Its concept was that a natural coagulant was used in primary treatment to significantly reduce turbidity and alum was used in secondary treatment at a minimum dose. This approach was successfully applied for treating bentonite turbid water. To verify if its concept is feasible for treating kaolin turbid water, this research was carried out. Experimental results showed that the novel approach achieved superior performance in reducing turbidity of treated water from 950 NTU to less than 5 NTU (i.e., satisfying the WHO drinking water standards) and decreasing alum dose required by 33 and 50% using palm bark and watermelon coagulants, respectively when compared with alum control treatment (i.e., replacing partially alum). The novel approach findings in current research are consistent with our previous research. Concluding its concept is validated with two types of bentonite and kaolin turbid waters. Further work needs to verify if its concept is feasible for treating river surface water. [ABSTRACT FROM AUTHOR]

Abstract Textile wastewater is among the most polluted types of industrial waste. Wastewater treatment in the textile industry is notoriously difficult because of the use of complex chemicals and dyes in the textile production steps, and conventional methods are not enough to treat these. Textile wastewater, known for its high pollution levels, poses challenges for treatment due to complex chemicals and dyes. A comparative study was conducted on simple ozonation and catalytic ozonation (CO) using alum to degrade methylene blue (MB). The authors analyzed various factors like time, pH, catalyst loading and ozone dosages during the study. Results showed that catalytic activity in ozonation depends on pH and ozone flow. The maximum MB elimination was achieved at pH 6.6 and 200 V ozone flow. Scanning electron microscopy (SEM) was used to characterize the surface morphology of the catalyst, Fourier transform infrared spectroscopy (FTIR) was used to identify the important functional groups, and energy dispersive X-ray spectroscopy (EDS) was used to characterize the catalyst's elemental composition. Compared to simple ozonation, CO showed higher removal in the initial phase. Real textile wastewater analysis confirmed the effectiveness of alum catalysts in achieving significant removal of MB (87%) through this novel cost-efficient process. Graphical Abstract

Textile wastewater is among the most polluted types of industrial waste. Wastewater treatment in the textile industry is notoriously difficult because of the use of complex chemicals and dyes in the textile production steps, and conventional methods are not enough to treat these. Textile wastewater, known for its high pollution levels, poses challenges for treatment due to complex chemicals and dyes. A comparative study was conducted on simple ozonation and catalytic ozonation (CO) using alum to degrade methylene blue (MB). The authors analyzed various factors like time, pH, catalyst loading and ozone dosages during the study. Results showed that catalytic activity in ozonation depends on pH and ozone flow. The maximum MB elimination was achieved at pH 6.6 and 200 V ozone flow. Scanning electron microscopy (SEM) was used to characterize the surface morphology of the catalyst, Fourier transform infrared spectroscopy (FTIR) was used to identify the important functional groups, and energy dispersive X-ray spectroscopy (EDS) was used to characterize the catalyst's elemental composition. Compared to simple ozonation, CO showed higher removal in the initial phase. Real textile wastewater analysis confirmed the effectiveness of alum catalysts in achieving significant removal of MB (87%) through this novel cost-efficient process. Highlights: Methylene Blue was treated with the catalytic ozonation method, with first ever use of alum as a novel ozonation catalyst. Both simple ozonation and catalytic ozonation were efficient treatment processes; however, maximum removal efficiency for Methylene Blue of 86% was achieved by alum based catalytic ozonation process. On optimizing pH to 6.6, maximum removal efficiencies for Methylene Blue improve to 85% and 99% for simple ozonation and catalytic ozonation processes, respectively. The improved results for catalytic ozonation are owed to predominant hydroxyl radical mechanism. [ABSTRACT FROM AUTHOR]

In this work, a "turn waste into treasure" strategy is proposed to synthesize Fe2O3 and use it as a lithium-ion battery (LIB) anode from green alum slag, a by-product of TiO2 production in the industry. Through this method, a heteroatom-doped Fe2O3 was obtained and showed a stable specific capacity of 1209 mA h g−1 at 500 mA g−1, which was superior to pure-Fe2O3 that was prepared in the laboratory. Besides, the full battery was assembled with Fe2O3 as the anode, and the specific capacity was maintained at 102 mA h g−1 after 100 cycles of stable cycling at 1.0 A g−1, showing excellent cycling and rate performance. This work offers a green low-cost and promising option for the development of LIBs anode materials and making TiO2 industrial production more economical and environmentally friendly. [ABSTRACT FROM AUTHOR]

Bio Farma has developed a recombinant protein subunit vaccine (IndoVac) that is indicated for active immunization in population of all ages. This article reported the results of the phase 3 immunogenicity and safety study in Indonesian adults aged 18 years and above. We conducted a randomized, active-controlled, multicenter, prospective intervention study to evaluate the immunogenicity and safety of IndoVac in adults aged 18 years and above. Participants who were SARS-CoV-2 vaccine-naïve received two doses of either IndoVac or control (Covovax) with 28 days interval between doses and were followed up until 12 months after complete vaccination. A total of 4050 participants were enrolled from June to August 2022 and received at least one dose of vaccine. The geometric mean ratio (GMR) of neutralizing antibody at 14 days after the second dose was 1.01 (95 % confidence interval (CI) 0.89–1.16), which met the WHO non-inferiority criteria for immunobridging (95 % CI lower bound > 0.67). The antibody levels were maintained through 12 months after the second dose. The incidence rate of adverse events (AEs) were 27.95 % in IndoVac group and 32.15 % in Covovax group with mostly mild intensity (27.70 %). The most reported solicited AEs were pain (14.69 %) followed by myalgia (7.48 %) and fatigue (6.77 %). Unsolicited AEs varied, with each of the incidence rate under 5 %. There were no serious AEs assessed as possibly, probably, or likely related to vaccine. IndoVac in adults showed favourable safety profile and elicited non-inferior immune response to Covovax. (ClinicalTrials.gov : NCT05433285, Indonesian Clinical Research Registry: INA-R5752S9). [ABSTRACT FROM AUTHOR]

For several years, we have been committed to exploring the potential of Bordetella pertussis-derived outer membrane vesicles (OMVBp) as a promising third-generation vaccine against the reemerging pertussis disease. The results of our preclinical trials not only confirm its protective capacity against B. pertussis infection but also set the stage for forthcoming human clinical trials. This study delves into the examination of OMVBp as an adjuvant. To accomplish this objective, we implemented a two-dose murine schedule to evaluate the specific immune response induced by formulations containing OMVBp combined with 3 heterologous immunogens: Tetanus toxoid (T), Diphtheria toxoid (D), and the SARS-CoV-2 Spike protein (S). The specific levels of IgG, IgG1, and IgG2a triggered by the different tested formulations were evaluated using ELISA in dose-response assays for OMVBp and the immunogens at varying levels. These assays demonstrated that OMVBp exhibits adjuvant properties even at the low concentration employed (1.5 mg of protein per dose). As this effect was notably enhanced at medium (3 mg) and high concentrations (6 mg), we chose the medium concentration to determine the minimum immunogen dose at which the OMV adjuvant properties are significantly evident. These assays demonstrated that OMVBp exhibits adjuvant properties even at the lowest concentration tested for each immunogen. In the presence of OMVBp, specific IgG levels detected for the lowest amount of antigen tested increased by 2.5 to 10 fold compared to those found in animals immunized with formulations containing adjuvant-free antigens (p<0.0001). When assessing the adjuvant properties of OMVBp compared to the widely recognized adjuvant alum, we detected similar levels of specific IgG against D, T and S for both adjuvants. Experiments with OMVs derived from E. coli (OMVE.coli) reaffirmed that the adjuvant properties of OMVs extend across different bacterial species. Nonetheless, it's crucial to highlight that OMVBp notably skewed the immune response towards a Th1 profile (p<0.05). These collective findings emphasize the dual role of OMVBp as both an adjuvant and modulator of the immune response, positioning it favorably for incorporation into combined vaccine formulations. [ABSTRACT FROM AUTHOR]

To simultaneously solve problems in an eco-friendly manner, introducing a waste residual as a sustainable conditioner to aid alum sludge dewatering is suggested as a cradle-to-cradle form of waste management. In this regard, the superiority of deep dewatering alum sludge with a powdered wood chip composite residual as a novel conditioner was explored, whereby traditional conventional conditioners, i.e., polyelectrolytes and lime, were substituted with powdered wood chips. Initially, Fe3O4 was prepared at the nanoscale using a simple co-precipitation route. Next, wooden waste was chemically and thermally treated to attain cellulosic fine powder. Subsequently, the resultant wood powder and Fe3O4 nanoparticles were mixed at 50 wt % to attain a wood powder augmented with iron, and this conditioner was labeled nano-iron-cellulose (nIC-Conditioner). This material (nIC-Conditioner) was mixed with hydrogen peroxide to represent a dual oxidation and skeleton builder conditioning substance. Characterization of the resultant conditioner was carried out using transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) transmittance spectrum analysis. The feasibility of the experimental results revealed that the moisture content in the sludge cake was lower after conditioning, and the capillary suction time (CST) was reduced to 78% compared to that of raw alum sludge after 5 min of dewatering time. Moreover, the optimal system parameters, including nIC-Conditioner and H2O2 concentrations, as well as the working pH, were optimized, and optimal values were recorded at 1 g/L and 200 mg/L for nIC-Conditioner and H2O2, respectively, with a pH of 6.5. Additionally, scanning electron microscope (SEM) analyses of the sludge prior to and after conditioning were conducted to verify the change in sludge molecules due to this conditioning technique. The results of this study confirm the sustainability of an alum sludge and waste management facility. [ABSTRACT FROM AUTHOR]

Neutrophil extracellular traps (NETs) have been identified as triggers for a self‐limited inflammatory reaction upon contact with nanoparticles within our bodies. This typically results in entrapping potentially harmful nano‐ or micro‐objects following an immune burst. The demand for potent adjuvants has led to research on particulate‐based adjuvants, particularly those that act via NET formation. Various particles, including hydrophobic nanoparticles, needle‐like microparticles, and other natural and artificial crystals, have been shown to induce NET formation, eliciting a robust humoral and cellular immune response toward co‐injected antigens. The NET formation was found to be the basis of the efficient use of alum as a vaccine adjuvant. Thus, nanoparticles with specific surface properties serve as NET‐stimulating adjuvants. In this mini‐review, we aim to summarize the current knowledge about the surface properties of particulate objects and the molecular pathways involved in inducing NET formation by neutrophils. Additionally, we discuss the potential use of nanoparticles for activating neutrophils in the tissues and the exploitation of such activation for enhancing vaccine adjuvants. [ABSTRACT FROM AUTHOR]

Background: Adjuvants are widely used to enhance and/or direct vaccine-induced immune responses yet rarely evaluated head-to-head. Our trial directly compared immune responses elicited by MF59 versus alum adjuvants in the RV144-like HIV vaccine regimen modified for the Southern African region. The RV144 trial of a recombinant canarypox vaccine vector expressing HIV env subtype B (ALVAC-HIV) prime followed by ALVAC-HIV plus a bivalent gp120 protein vaccine boost adjuvanted with alum is the only trial to have shown modest HIV vaccine efficacy. Data generated after RV144 suggested that use of MF59 adjuvant might allow lower protein doses to be used while maintaining robust immune responses. We evaluated safety and immunogenicity of an HIV recombinant canarypox vaccine vector expressing HIV env subtype C (ALVAC-HIV) prime followed by ALVAC-HIV plus a bivalent gp120 protein vaccine boost (gp120) adjuvanted with alum (ALVAC-HIV+gp120/alum) or MF59 (ALVAC-HIV+gp120/MF59) or unadjuvanted (ALVAC-HIV+gp120/no-adjuvant) and a regimen where ALVAC-HIV+gp120 adjuvanted with MF59 was used for the prime and boost (ALVAC-HIV+gp120/MF59 coadministration). Methods and findings: Between June 19, 2017 and June 14, 2018, 132 healthy adults without HIV in South Africa, Zimbabwe, and Mozambique were randomized to receive intramuscularly: (1) 2 priming doses of ALVAC-HIV (months 0 and 1) followed by 3 booster doses of ALVAC-HIV+gp120/MF59 (months 3, 6, and 12), n = 36; (2) 2 priming doses of ALVAC-HIV (months 0 and 1) followed by 3 booster doses of ALVAC-HIV+gp120/alum (months 3, 6, and 12), n = 36; (3) 4 doses of ALVAC-HIV+gp120/MF59 coadministered (months 0, 1, 6, and 12), n = 36; or (4) 2 priming doses of ALVAC-HIV (months 0 and 1) followed by 3 booster doses of ALVAC-HIV+gp120/no adjuvant (months 3, 6, and 12), n = 24. Primary outcomes were safety and occurrence and mean fluorescence intensity (MFI) of vaccine-induced gp120-specific IgG and IgA binding antibodies at month 6.5. All vaccinations were safe and well-tolerated; increased alanine aminotransferase was the most frequent related adverse event, occurring in 2 (1.5%) participants (1 severe, 1 mild). At month 6.5, vaccine-specific gp120 IgG binding antibodies were detected in 100% of vaccinees for all 4 vaccine groups. No significant differences were seen in the occurrence and net MFI of vaccine-specific IgA responses between the ALVAC-HIV+gp120/MF59-prime-boost and ALVAC-HIV+gp120/alum-prime-boost groups or between the ALVAC-HIV+gp120/MF59-prime-boost and ALVAC-HIV+gp120/MF59 coadministration groups. Limitations were the relatively small sample size per group and lack of evaluation of higher gp120 doses. Conclusions: Although MF59 was expected to enhance immune responses, alum induced similar responses to MF59, suggesting that the choice between these adjuvants may not be critical for the ALVAC+gp120 regimen. Trial registration: HVTN 107 was registered with the South African National Clinical Trials Registry (DOH-27-0715-4894) and ClinicalTrials.gov (NCT03284710). Zoe Moodie and colleagues investigate the safety and immunogenicity of a subtype C ALVAC-HIV vaccine prime plus bivalent subtype C gp120 vaccine boost adjuvanted with MF59 or alum in healthy adults without HIV. Author summary: Why was this study done?: Vaccines may use an adjuvant to help the body produce a stronger immune response. Results from animal studies suggested that the MF59 adjuvant generates better immunogenicity than the alum adjuvant when given as part of an HIV vaccine and could also allow a lower dose of protein to be used. Our clinical trial was done to directly assess in humans whether MF59 leads to better immune responses than alum when given with protein in a subtype C canarypox vaccine (ALVAC-HIV) prime followed by ALVAC-HIV plus a bivalent gp120 protein vaccine boost (gp120). What did the researchers do and find?: Vaccines were safe and well-tolerated over the 18 months of follow-up. 100% of vaccinees had vaccine-specific gp120 IgG binding antibodies at month 6.5. Immune responses for the ALVAC-HIV+gp120/MF59 group and the ALVAC-HIV+gp120/alum group were similar. What do these findings mean?: Contrary to expectation, the choice between MF59 and alum does not seem critical to the immune responses assessed in the peripheral blood for this subtype C ALVAC-HIV+gp120 prime-boost regimen. The main limitations of our study were the small vaccine group sample sizes and that higher doses of gp120 protein were not evaluated. [ABSTRACT FROM AUTHOR]

Clostridioides difficile infection (CDI) is a serious healthcare-associated disease, causing symptoms such as diarrhea and pseudomembranous colitis. The major virulence factors responsible for the disease symptoms are two secreted cytotoxic proteins, TcdA and TcdB. A parenteral vaccine based on formaldehyde-inactivated TcdA and TcdB supplemented with alum adjuvant, has previously been investigated in humans but resulted in an insufficient immune response. In search for an improved response, we investigated a novel toxin inactivation method and a novel, potent adjuvant. Inactivation of toxins by metal-catalyzed oxidation (MCO) was previously shown to preserve neutralizing epitopes and to annihilate reversion to toxicity. The immunogenicity and safety of TcdA and TcdB inactivated by MCO and combined with a novel carbohydrate fatty acid monosulphate ester-based (CMS) adjuvant were investigated in rabbits. Two or three intramuscular immunizations generated high serum IgG and neutralizing antibody titers against both toxins. The CMS adjuvant increased antibody responses to both toxins while an alum adjuvant control was effective only against TcdA. Systemic safety was evaluated by monitoring body weight, body temperature, and analysis of red and white blood cell counts shortly after immunization. Local safety was assessed by histopathologic examination of the injection site at the end of the study. Body weight gain was constant in all groups. Body temperature increased up to 1 ˚C one day after the first immunization but less after the second or third immunization. White blood cell counts, and percentage of neutrophils increased one day after immunization with CMS-adjuvanted vaccines, but not with alum. Histopathology of the injection sites 42 days after the last injection did not reveal any abnormal tissue reactions. From this study, we conclude that TcdA and TcdB inactivated by MCO and combined with CMS adjuvant demonstrated promising immunogenicity and safety in rabbits and could be a candidate for a vaccine against CDI. [ABSTRACT FROM AUTHOR]

Conventional chemical coagulants are used for potable water treatment, which requires unsustainable mining and transformation of raw materials for their production with costly sludge disposal. Natural coagulants are potential alternatives to chemical coagulants. This study emphasized the use of natural eco-friendly coagulants (oat and onion seed) for turbid water treatment. Coagulant seeds were characterized by different techniques such as SEM, EDX, FTIR, and zeta potential analyzer. Response surface method (RSM) was used to optimize the coagulation process. The effect of initial water turbidity, pH, coagulant seed extract dose, slow mix time, and settling time on the efficiency of turbidity removal was studied. The relation between removal efficiency and the main three significant operational parameters (initial water turbidity, pH, and coagulant extract dose) was fitted to a quadratic model. The optimal removal was achieved at alkaline and neutral media (pH = 7–11 for oat extract and pH = 7–9 for onion extract). The turbidity removal efficiency was 99% using 6 ml/L oat extract dose and 98.9% using 4 ml/L onion extract dose at pH equal to 8 and initial turbidity of 190 NTU. The effect of using oat extract or onion extract with alum was studied, and the results indicated that the optimum dose was found to be 1 ml/L of a natural extract with 10 mg/L alum. Furthermore, oat and onion extract aids were able to decrease the organic load in the treated water. Oat and onion seed extracts were proven to be efficient and eco-friendly natural coagulants or coagulant aids for surface water treatment. [ABSTRACT FROM AUTHOR]

The treatment of dye laden wastewater (DLW) was investigated using alum, CA${C}_A$ and Luffa cylindrica seed extract, CLCSE${C}_{LCSE}$. Cibacron green dye and distilled water were used to simulate the DLW in the laboratory. The optimum conditions for the removal of color and chemical oxygen demand (COD) from DLW was determined using both regular and response surface methodology, RSM‐Jar tests with coagulant dosage, DLW pH, stirring time, and temperature as the independent variables. Instrumental characterization of the coagulant and produced sludge were studied. Individual effect using regular Jar test, CA${C}_A$ and CLCSE${C}_{LCSE}$ had best color removal efficiency of 98.4% (2 g/L, pH 8, 30 min and 55°C), and 97.7% (5 g/L, pH 2, 20 min and 55°C), respectively. Also, for COD removal efficiency, CA${C}_A$ and CLCSE${C}_{LCSE}$ had 85.6% (2 g/L, pH 10 and 30 min), and 88.1% (4 g/L, pH 4 and 30 min), respectively. Applying RSM‐Jar test, 99.6% and 88.7% color and COD removal efficiency were achieved with 4 g/L CLCSE${C}_{LCSE}$, 0.25 g/L CA${C}_A$, pH 8, and 40°C and 5 g/L CLCSE${C}_{LCSE}$, 0.40 g/L CA${C}_A$, pH 6, and 40°C, respectively. The results indicated that the combination of both coagulants (CA+LCSE${C}_{A + LCSE}$) performed maximally with highly reduced alum dosage, exposing the novelty of the work. Hence, the novelty of the work is the use of minimal quantity of alum and Luffa cylindrica seed extract for effective removal color and COD from the DLW. [ABSTRACT FROM AUTHOR]

The production of drinking water often leads to the creation of a high residual concentration of aluminum when mineral salts are used. This concentration must be carefully monitored and controlled. A study was carried out at a drinking water production facility situated in Al Hoceima, Northern Morocco.Throughout this investigation, the treated water from the plant underwent comparative testing, evaluating the effectiveness of chitosan as a flocculant and bentonite as an adjuvant with inorganic coagulants (aluminum sulfate and ferric chloride). The objective was to reduce the residual aluminum concentration in the water after treatment while minimizing the use of inorganic coagulant. Coagulation-flocculation tests, employing a jar test, optimized coagulant dosage while enhancing turbidity and oxidability removal rates.The coagulation-flocculation tests showed that the combination of aluminum sulfate with 10 mg/L of chitosan and 140 mg/L of bentonite, proved to be significantly more effective. Compared to traditional treatment methods using aluminum sulfate alone, this combination reduced the residual aluminum concentration by 38.88%. The optimal parameters were as follows: aluminum sulfate dose of 10 mg/L, a pH of 7.83, an aluminum concentration of

This study aims to recover the used coagulants from two water treatment plants via acidification technique. The water treatment sludge (WTS) was acidified with sulfuric acid (H2SO­4) at variable normalities (0.5, 1, 1.5, 2.0 and 2.5 N). The surface morphology and functionalities of both recovered coagulants were analysed using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR). The performance of recovered coagulants was tested for turbidity removal in surface water treatment at different coagulant dosages and pH. It was found that the optimum normality of H2SO4 for recovered alum was 1.5 N, where 66% turbidity removal was recorded. The recovered PAC treated with 1.0 N H2SO4 indicated high turbidity removal percentage, which was 50.5%. The turbidity removal increased with increasing coagulant dosage. More than 80% turbidity removal was achieved with 40 mg/L dosage of recovered alum and recovered PAC. Maximum removal (85%) was observed with 50 mg/L dosage of recovered alum. For commercial coagulant, the turbidity removal was higher, with a difference of up to 6% in favor of recovered alum. The potential reuse of coagulants can be explored in order to reduce the operating costs and promotes the reduction of WTS disposal. HIGHLIGHTS Coagulation in water treatment process generates a large amount of sludge.; Water treatment sludge (WTS) are commonly discharged into rivers.; Coagulant recovery from WTS can reduce environmental impact.; Coagulant recovery is a great approach to support water circular economy.;

The continuous evolution and mutation of SARS-CoV-2 have highlighted the need for more effective vaccines. In this study, CpG, MF59-like, and Alum adjuvant Delta strain inactivated SARS-CoV-2 vaccines were prepared, and the immunogenicity of these vaccines in mice was evaluated. The Delta + MF59-like vaccine group produced the highest levels of S- and RBD-binding antibodies and live Delta virus neutralization levels after one shot of immunization, while mice in the Delta + Alum vaccine group had the highest levels of these antibodies after two doses, and the Delta + MF59-like and Delta + Alum vaccine groups produced high levels of cross-neutralization antibodies against prototype, Beta, and Gamma strain SARS-CoV-2 viruses. There was no significant decrease in neutralizing antibody levels in any vaccine group during the observation period. CpG, MF59-like, and Alum adjuvant Delta strain inactivated SARS-CoV-2 vaccines excited different antibody subtypes compared with unadjuvanted vaccines; the Delta + CpG vaccine group had a higher proportion of IgG2b antibodies, indicating bias towards Th1 immunity. The proportions of IgG1 and IgG2b in the Delta + MF59-like vaccine group were similar to those of the unadjuvanted vaccine. However, the Delta + Alum vaccine group had a higher proportion of IgG1 antibodies, indicating bias towards Th2 immunity. Antigen-specific cytokine secretion CD4/8+ T cells were analyzed. In conclusion, the results of this study show differences in the immune efficacy of CpG, MF59-like, and Alum adjuvant Delta strain inactivated SARS-CoV-2 vaccines in mice, which have significant implications for the selection strategy for vaccine adjuvants. [ABSTRACT FROM AUTHOR]

Salt produced from seawater evaporation contains harmful microplastics (MP). For this reason, a technology that can remove MP from seawater using coagulation and filtration techniques is needed. The purpose of this study is to utilize alum as a coagulant and sand as a filtration media to reduce MP pollution in seawater as a source of raw material for salt making. Seawater from Buo Bay, Padang City, Indonesia was taken as raw material for salt production. The MP abundance of salt made from seawater without alum and sand treatment was found to be 400 particles/kg. To reduce the abundance of MP in the salt, we varied the alum concentration (0.1; 0.3; and 0.5 g/L) and sand particle size (=2, =1-<2, and <1 mm). From the results obtained, the optimal condition is an alum concentration of 0.5 g/L and sand particle size is <1 mm. The optimal condition of salt made from seawater in treatment H obtained MP abundance from 400 particles/kg to 30 particles/kg with an MP reduction efficiency of 92.5%. Visual analysis using optical trinocular microscopy found 4 forms of MP, namely: fragments (51.13%), fibers (28.95%), films (15.41%), and pellets (4.05%). Rewith the most dominant MP size found was >100-300 µm. The results of ATR-FTIR analysis identified the types of MP as Polyethylene (14.28%), Polyethylene Terephthalate (42.85%), Polypropylene (14.28%), and Polyamide (28.57%). [ABSTRACT FROM AUTHOR]

The study aimed to determine the growth performance, carcass characteristics, haematological and histological indices of broiler finisher birds reared on varying levels of alum treated litter. A total of 300 mixed sex of broiler birds at 4 weeks of age were allotted to 4 treatments groups in a completely randomized design (CRD)with 5 replicates of 15 birds each. Treatment 1 served as the control, while treatments 2, 3 and 4 contained (200g, 400g, and 600g) of alum per 5kg liter respectively. Results showed that the body weight gain, ADWG, FCR, feed intake and ADFI values were higher (p<0.05) in favor of the treatment groups (T2, T3 and T4). Treatment groups had better (p<0.05) carcass and organ quality than the control group. In hematology results, it was only the values for WBC were significant (p<0.05) across the treatment and the value was highest (p<0.05) in control birds. Furthermore, histological indices determined were not significant (p>0.05). It was therefore, concluded that the treatment of broiler litter with alum up to 600g/5kg litter had no adverse effect on their performance and can be employed as a litter management protocols for improving performance in broiler production. [ABSTRACT FROM AUTHOR]

Fast urbanization has boosted the automotive sector in cities. Two service stations collected car wash water, which was analyzed thoroughly. pH, total suspended solids, total dissolved solids, oil & grease, chemical oxygen demand (COD), biochemical oxygen demand, chlorides, and sulfates were measured. Chemical treatment used alum, while physical treatment used sawdust and sugarcane bagasse. Sample A contains wash water from 2- and 4-wheelers, while sample B has only 4-wheeler wash water. The empirical results show that physical treatment filter bed porosity, surface area, and height affect substance sorption. COD reduction for wood dust and sugarcane in sample A is 91.59% and 90.70%, while in sample B it is 81.40% and 86.70%. COD and oil & grease concentration were measured at 3, 6, and 9 cm filter columns. The percentage reduction in COD and oil & grease was related to filter material penetration. COD, oil, and grease removal effectiveness increases with filter bed depth. Alum's COD reduction is 89.10 in sample A and 87.50 in sample B, according to coagulant dosage. Alum removes 92.40% and 93.20% of oil & grease in samples A and B. Sample A used sugarcane bagasse and sawdust to remove 94.31% and 93.40% of oil & grease. Removal percentages in sample B were 92.67% and 93.80%. Sugarcane and wood dust reduces COD and oil & grease more in sample A than sample B. Sample A had a lower alum removal rate than sample B. [ABSTRACT FROM AUTHOR]

Leptospirosis is a globally significant zoonotic disease. The current inactivated vaccine offers protection against specific serovars but does not provide complete immunity. Various surface antigens, such as Leptospira immunoglobulin-like proteins (LigA and LigB), have been identified as potential subunit vaccine candidates. However, these antigens require potent adjuvants for effectiveness. Bacterial lipopolysaccharides (LPSs), including lipid A, are a well-known immunostimulant, and clinical adjuvants often contain monophosphoryl lipid A (MPLA). Being less endotoxic, we investigated the adjuvant properties of lipid A isolated from L. interrogans serovar Pomona (PLA) in activating innate immunity and enhancing antigen-specific adaptive immune responses. PLA activated macrophages to a similar degree as MPLA, albeit at a higher dose, suggesting that it is less potent in stimulation than MPLA. Mice immunized with a variable portion of LigA (LAV) combined with alum and PLA (LAV-alum-PLA) exhibited significantly higher levels of LAV-specific humoral and cellular immune responses compared to alum alone but similar to those induced by alum-MPLA. The adjuvant activity of PLA resembles that of MPLA and is primarily achieved through the increased recruitment, activation, and uptake of antigens by innate immune cells. Furthermore, like MPLA, PLA formulation establishes a long-lasting memory response. Notably, PLA demonstrated superior potency than MPLA formulation and provided sterilizing immunity against the leptospirosis in a hamster model. Overall, our study sheds light on the adjuvant properties of Leptospira lipid A and offers promising avenues for developing LPS-based vaccines against this devastating zoonotic disease. [ABSTRACT FROM AUTHOR]

There are some concerns about the safety of live attenuated yellow fever vaccines (YF–live), particularly viscerotropic adverse events, which have a high mortality rate. The cellular production of the vaccine will not cause these adverse effects and has the potential to extend applicability to those who have allergic reactions, immunosuppression, and age. In this study, inactivated yellow fever (YF) was prepared and adsorbed with Alum/CpG. The cellular and humoral immunities were investigated in a mouse model. The results showed that Alum/CpG (20 μg/mL) could significantly increase the binding and neutralizing activities of the antibodies against YF. Moreover, the antibody level at day 28 after one dose was similar to that of the attenuated vaccine, but significantly higher after two doses. At the same time, Alum/CpG significantly increased the levels of IFN-γ and IL-4 cytokines. [ABSTRACT FROM AUTHOR]

The increased urge to protect the environment from the harsh impact of CO2 emissions has led to the development of a new technology named CCS abbreviated as carbon capture and storage. CCS is a crucial technology that focuses on emission removal from the source or directly from the air. CCS is a three-step process including carbon capture, transportation, and storage with CO2 storage (CO2) as an important step as it permanently removes the CO2 from the atmosphere. CO2 can be stored in geological formations like shale, coal, and basalts with shale displaying high adsorption capacity towards CO2 gas making it a suitable site for CO2 storage. Shale is a fine-grained rock having high porosity that follows an adsorption mechanism for CO2 storage, and thus, it becomes crucially important to describe the adsorption process in shale rock. The adsorption mechanism can be studied by numerous isotherm models namely monolayer adsorption-based Langmuir, classical statistical mechanics-based Freundlich, Temkin, and micro-pore filling adsorption D-R isotherm models. This article applies isotherm models on the alum shale collected from Denmark for gases CO2, N2, and CH4 at temperatures of 195 K, 77 K, and 112 K at a depth of 9 m and predicts the best-fit isotherm model for the alum shale based on a linear regression method. The study finds that the Langmuir isotherm model was suitable for CH4 and N2 gas with accuracy of 96.86% and 95.79% and the Freundlich model of isotherm was best suitable for CO2 gas with an accuracy of 99.45%. Langmuir isotherm best describes the adsorption based on the gas–solid phase and is utilized for quantifying the adsorptive capacity of different adsorbents. [ABSTRACT FROM AUTHOR]

This study aimed to optimize the per‐O‐acetylation, deacetylation, and benzylidene protection of carbohydrates and investigate the scope of saccharides via alum‐catalysis. Initially, optimization was based on the previously reported PTA‐catalyzed acetylation of glucose, but alum‐catalysis required heating conditions. The study expanded to investigate the per‐O‐acetylation of different saccharides, including galactose and mannose, which produced stereoisomeric mixtures. The study proposed a plausible reaction mechanism for the isomerization observed in galactose and xylose after acetylation. The mechanism involved chelation of the active alum‐catalyst with the O‐6 position and β‐type O‐1 position of galactopyranose to form a transition state complex via ring opening. This study investigated the generality of the de‐O‐acetylation method on a variety of carbohydrates using per‐O‐acetyl saccharide substrates bearing different functional groups. The direct protection of benzylidene in addition to acetylation/de‐O‐acetylation with a focus on optimizing reaction conditions for the synthesis of carbohydrate derivatives. Overall, the study offers insights into optimizing de‐acetylation, per‐O‐acetylation, and benzylidene protection investigating the scope of saccharides via alum‐catalysis. [ABSTRACT FROM AUTHOR]

For several years, we have been committed to exploring the potential of Bordetella pertussis-derived outer membrane vesicles (OMVBp) as a promising third-generation vaccine against the reemerging pertussis disease. The results of our preclinical trials not only confirm its protective capacity against B. pertussis infection but also set the stage for forthcoming human clinical trials. This study delves into the examination of OMVBp as an adjuvant. To accomplish this objective, we implemented a two-dose murine schedule to evaluate the specific immune response induced by formulations containing OMVBp combined with 3 heterologous immunogens: Tetanus toxoid (T), Diphtheria toxoid (D), and the SARS-CoV-2 Spike protein (S). The specific levels of IgG, IgG1, and IgG2a triggered by the different tested formulations were evaluated using ELISA in dose-response assays for OMVBp and the immunogens at varying levels. These assays demonstrated that OMVBp exhibits adjuvant properties even at the low concentration employed (1.5 μg of protein per dose). As this effect was notably enhanced at medium (3 μg) and high concentrations (6 μg), we chose the medium concentration to determine the minimum immunogen dose at which the OMV adjuvant properties are significantly evident. These assays demonstrated that OMVBp exhibits adjuvant properties even at the lowest concentration tested for each immunogen. In the presence of OMVBp, specific IgG levels detected for the lowest amount of antigen tested increased by 2.5 to 10 fold compared to those found in animals immunized with formulations containing adjuvant-free antigens (p