Search

Your search keyword '"Altman K"' showing total 518 results
Start Over Author "Altman K"
518 results on '"Altman K"'

8. Taxanes convert regions of perturbed microtubule growth into rescue sites

Author

Rai, A., Liu, T., Glauser, S., Katrukha, E.A., Estévez-Gallego, J., Rodríguez-García, R., Fang, W.-S., Díaz, J.F., Steinmetz, M.O., Altman, K.-H., Kapitein, L.C., Moores, Carolyn A., and Akhmanova, A.

Subjects
bcs
Abstract

Microtubules are polymers of tubulin dimers, and conformational transitions in the microtubule \ud lattice drive microtubule dynamic instability and affect various aspects of microtubule function.\ud The exact nature of these transitions and their modulation by anti -cancer drugs such as Taxol and\ud epothilone, which can stabilize microtubules but also perturb their growth, are poorly understood.\ud Here, we directly visualize the action of fluorescent Taxol and epothilone derivatives and show\ud that microtubules can transition to a state that triggers cooperative drug binding to form regions\ud with altered lattice conformation. Such regions emerge at growing microtubule ends that are in a\ud pre-catastrophe state and inhibit microtubule growth and shortening. Electron microscopy and in\ud vitro dynamics data indicate that taxane accumulation zones represent incomplete tubes that can\ud persist, incorporate tubulin dimers and repeatedly induce microtubule rescues. Thus, taxanes\ud modulate the material properties of microtubules by converting destabilized growing microtubule\ud ends into regions resistant to depolymerization.

Published
2020

Catalog

Books, media, physical & digital resources
See catalog results