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Insect pest control poses a global challenge, affecting public health, food safety, and the environment. Diseases transmitted by mosquitoes are expanding beyond tropical regions due to climate change. Agricultural pests further exacerbate economic losses by damaging crops. The Sterile Insect Technique (SIT) emerges as an eco-friendly alternative to chemical pesticides, involving the sterilization and release of male insects to curb population growth. This work focuses on the automation of the analysis of field ovitraps used to follow-up a SIT program for the Aedes albopictus mosquito in the Valencian Community, Spain, funded by the Conselleria de Agricultura, Agua, Ganaderia y Pesca. Previous research has leveraged deep learning algorithms to automate egg counting in ovitraps, yet faced challenges such as manual handling and limited analysis capacity. Innovations in our study include classifying eggs as hatched or unhatched and reconstructing ovitraps from partial images, mitigating issues of duplicity and cut eggs. Also, our device can analyze multiple ovitraps simultaneously without the need of manual replacement. This approach significantly enhances the accuracy of egg counting and classification, providing a valuable tool for large-scale field studies. This document describes part of the work of the project Application of Industry 4.0 techniques to the production of tiger mosquitoes for the Sterile Insect Technique (MoTIA2,IMDEEA/2022/70), financed by the Valencian Institute for Business Competitiveness (IVACE) and the FEDER funds. The participation of J.Naranjo-Alcazar, J.Grau-Haro and P.Zuccarello has been possible thanks to funding from IVACE and FEDER funds. The participation of D.Almenar has been financed by the Conselleria de Agricultura, Agua, Ganaderia y Pesca of the Generalitat Valenciana and the Subdireccion de Innovacion y Desarrollo de Servicios (TRAGSA group).

The Sterile Insect Technique (SIT) is a biological pest control technique based on the release into the environment of sterile males of the insect species whose population is to be controlled. The entire SIT process involves mass-rearing within a biofactory, sorting of the specimens by sex, sterilization, and subsequent release of the sterile males into the environment. The reason for avoiding the release of female specimens is because, unlike males, females bite, with the subsequent risk of disease transmission. In the case of Aedes mosquito biofactories for SIT, the key point of the whole process is sex separation. This process is nowadays performed by a combination of mechanical devices and AI-based vision systems. However, there is still a possibility of false negatives, so a last stage of verification is necessary before releasing them into the environment. It is known that the sound produced by the flapping of adult male mosquitoes is different from that produced by females, so this feature can be used to detect the presence of females in containers prior to environmental release. This paper presents a study for the detection of females in Aedes mosquito release vessels for SIT programs. The containers used consist of PVC a tubular design of 8.8cm diameter and 12.5cm height. The containers were placed in an experimental setup that allowed the recording of the sound of mosquito flight inside of them. Each container was filled with 250 specimens considering the cases of (i) only male mosquitoes, (ii) only female mosquitoes, and (iii) 75% males and 25% females. Case (i) was used for training and testing, whereas cases (ii) and (iii) were used only for testing. Two algorithms were implemented for the detection of female mosquitoes: an unsupervised outlier detection algorithm (iForest) and a one-class SVM trained with male-only recordings., Comment: Accepted EUSIPCO 2024