Search

Your search keyword '"Allsopp, Elleunorah"' showing total 15 results
Start Over Author "Allsopp, Elleunorah"
15 results on '"Allsopp, Elleunorah"'

1. Barcoding of parasitoid wasps (Braconidae and Chalcidoidea) associated with wild and cultivated olives in the Western Cape of South Africa

Author

Powell, Chante, Caleca, Virgilio, Sinno, Martina, Staden, Michaela van, Noort, Simon van, Rhode, Clint, Allsopp, Elleunorah, and Asch, Barbara van

Subjects
Oleaceae -- Diseases and pests, Wasps -- Genetic aspects, DNA barcoding -- Methods, Fruit flies (Tephritidae), Bar codes, Insects, DNA, Agricultural pests, Biological sciences
Abstract

Wild and cultivated olives harbor and share a diversity of insects, some of which are considered agricultural pests, such as the olive fruit fly. The assemblage of olive-associated parasitoids and seed wasps is rich and specialized in sub-Saharan Africa, with native species possibly coevolving with their hosts. Although historical entomological surveys reported on the diversity of olive wasp species in the Western Cape Province of South Africa, no comprehensive study has been performed in the region in the molecular era. In this study, a dual approach combining morphological and DNA-based methods was used for the identification of adult specimens reared from olive fruits. Four species of Braconidae and six species of Chalcidoidea were identified, and DNA barcoding methodologies were used to investigate conspecificity among individuals, based on randomly selected representative specimens. Morphological identifications were congruent with DNA data, as NJ and ML trees correctly placed the sequences for each species either at the genus or species level, depending on the available taxa coverage, and genetic distances strongly supported conspecificity. No clear evidence of cryptic diversity was found. Overall seed infestation and parasitism rates were higher in wild olives compared to cultivated olives, and highest for Eupelmus spermophilus and Utetes africanus. These results can be used for early DNA-based detection of wasp larvae in olives and to further investigate the biology and ecology of these species. Key words: Braconidae, Chalcidoidea, DNA barcoding, olives, species identification. Les olives sauvages et cultivees abritent et partagent une grande diversite d'insectes, dont certains sont consideres comme des ravageurs, comme la mouche de l'olive. Il existe une riche diversite de parasitoides et de guepes seminivores specialises associes aux oliviers en Afrique subsaharienne, dont plusieurs especes indigenes qui auraient co-evolue avec leurs hotes. Bien que des enquetes entomologiques historiques aient rapporte la diversite des guepes de l'olive dans la province du Cap-Occidental en Afrique du Sud, aucune etude approfondie n'a ete realisee dans cette region depuis l'avenement de methodes moleculaires. Dans ce travail, une approche double combinant des methodes morphologiques et basees sur l'ADN ont ete employees pour identifier des specimens adultes eleves sur des olives. Quatre especes de Braconidae et six especes de Chalcidoidea ont ete identifiees sur la base de la morphologie et des methodes de codage a barres de l'ADN ont ete employees pour etudier la conspecificite chez des individus choisis au hasard parmi des specimens representatifs. Les identifications morphologiques etaient en accord avec les donnees moleculaires car les arbres NJ et ML ont correctement place les sequences de chacune des especes, soit en fonction du genre ou de l'espece selon la couverture des taxons, et les distances genetiques ont fortement supporte la conspecificite. Aucune evidence claire de diversite cryptique n'a ete trouvee. Globalement, les niveaux d'infestation des graines et les taux de parasitisme etaient plus eleves chez les olives sauvages que chez les olives cultivees, atteignant un sommet chez l'Eupelmus spermophilus et l'Utetes africanus. Ces resultats pourront servir a la detection moleculaire precoce des larves de guepes dans les olives et pour etudier la biologie et l'ecologie de ces especes. [Traduit par la Redaction] Mots-cles : Braconidae, Chalcidoidea, codage a barres de l'ADN, olives, identification des especes., Introduction The wild olive tree (Olea europaea L. subsp. cuspidata) is closely related to the cultivated olive tree (Olea europaea L. subsp. europaea var. europaea) (Green 2002). The subspecies is [...]

Published
2019
Full Text
View/download PDF

11. Barcoding of parasitoid wasps (Braconidae and Chalcidoidea) associated with wild and cultivated olives in the Western Cape of South Africa 1

Author

Powell, Chante, Caleca, Virgilio, Sinno, Martina, van Staden, Michaela, van Noort, Simon, Rhode, Clint, Allsopp, Elleunorah, van Asch, Barbara, Powell, Chante, Caleca, Virgilio, Sinno, Martina, van Staden, Michaela, van Noort, Simon, Rhode, Clint, Allsopp, Elleunorah, and van Asch, Barbara

Subjects
Braconidae, Chalcidoidea, Settore AGR/11 - Entomologia Generale E Applicata, codage à barres de l’ADN, species identification, DNA barcoding, identification des espèce, olive
Abstract

Wild and cultivated olives harbor and share a diversity of insects, some of which are considered agricultural pests, such as the olive fruit fly. The assemblage of olive-associated parasitoids and seed wasps is rich and specialized in sub-Saharan Africa, with native species possibly coevolving with their hosts. Although historical entomological surveys reported on the diversity of olive wasp species in the Western Cape Province of South Africa, no comprehensive study has been performed in the region in the molecular era. In this study, a dual approach combining morphological and DNA-based methods was used for the identification of adult specimens reared from olive fruits. Four species of Braconidae and six species of Chalcidoidea were identified, and DNA barcoding methodologies were used to investigate conspecificity among individuals, based on randomly selected representative specimens. Morphological identifications were congruent with DNA data, as NJ and ML trees correctly placed the sequences for each species either at the genus or species level, depending on the available taxa coverage, and genetic distances strongly supported conspecificity. No clear evidence of cryptic diversity was found. Overall seed infestation and parasitism rates were higher in wild olives compared to cultivated olives, and highest for Eupelmus spermophilus and Utetes africanus. These results can be used for early DNA-based detection of wasp larvae in olives and to further investigate the biology and ecology of these species.

Published
2019

13. Heliothrips sylvanus Faure (Thysanoptera: Thripidae) confirmed as a potential economic pest of sweet and astringent persimmon in South Africa

Author

Persley, Denis, Wilson, Calum, Thomas, John, Sharman, Murray, Tree, Desley, Adkins, Scott, Karthikeyan, Gandhi, Damayanthi, Tri, Kodetham, Gopinath, Riley, David J., Naidu, Rayapati A., Allsopp, Elleunorah, Knipe, Muriel C., Bag, S., Druffel, K.L., Rondon, S., Riley, D.G., Pappu, Hanu R., Baker, Greg J., Powis, K., Crisp, P., Broughton, Sonya, Chilman, L., Burfield, Tony, Chiemsombat, Pissawan, Khankhum, Surasak, Hongprayoon, Ratchanee, Ciuffo, M., Turina, Massimo, Collins, Dom W., Glover, R., Boonham, N., Davidson, Melanie, Butler, R., Sansom, C., Perry, N., Teulon, D., De Facci, Monica, Chapman, T.W., Anderbrant, O., De Kogel, William Jan, Griepink, F.C., van Tol, R.W.H.M., Teulon, D.A.J., De Oliveira, V.C., Bartasson, L., Ribeiro, B.M., Resende, Renato, Dianese, E.C., Fonseca, M.E.N., Goldbach, R., Kormelink, R., Inoue-Nagata, A.K., Boiteux, L.S., Resende, Renato O., Diffie, Stan, Riley, David G., Dong, J-H., Zhang, Z-K., Yin, Y-Y., Cheng, X-F., Ding, M., Fang, Q., Fedor, Peter, Hammersteinová, I., Doričová, M., Sierka, W., Vaňhara, J., Havel, J., Majzlan, O., Greg Fonsah, Esendugue, Sparks, Stormy, Langston, David, Funderburk, Joe, Reitz, Stuart, Gitaitis, R.D., Csinos, A.S., Nischwitz, C., Mullis, Stephen W., Gunawardana, Disna, George, Sherly, Herron, Grant, Mo, Jianhua, Horne, Paul A., Page, Jessica, Jagadish, K., Vani, Akella, Nirmal Kumar, B.J., Kumar Jain, Rakesh, Mandal, B., Jones, Roger A.C., Coutts, Brenda, Kay, Iain, Heisswolf, S., Kirk, William D.J., Hamilton, James G. C., Koschier, Elisabeth H., Riefler, J., Kunkalikar, S.R., Sudarsana, P, Bhanupriya, M., Rajagopalan, Prem A., Yeh, S.D., Naidu, R.A., Zher, Usha B., Ravi, K.S., Kyamanywa, Samuel, Ochwo, M.K.N.S., Karungi, J., Rayapati, Naidu A., Ssemwogere, C., Riley, David, Li, Weimin, Fewandowski, Dennis J., Hilf, Mark E., Lister, R., Nielsen, M-C., Fletcher, J., Timmerman-Vaughan, G., Fimmerman-Vaughan, G., Fletcher, John, Lu, Yaobin, Zhang, Zhijun, Margaria, P., Bosco, F., Mautino, G., Tavella, F., McDougall, Sandra, Milinkovic, M., Slater, T., Rodoni, Brendon, Minaei, Kambiz, Mound, Laurence, Baker, G., Herron, G., Mound, L., Murai, Famotsu, Watanabe, Hiroki, Foriumi, Wataru, Adati, Faro, Okajima, Shuji, Nakao, Shiro, Chikamori, C., Schwarz, M., Nault, Brian, Hsu, C., Shelton, A., Fuchs, M., Hoepting, C., Newman, Julie P., Robb, Karen F., Nielsen, Mette, Hartnett, D.E., Pedley, R.I.F., Lewis, K., Srinivasan, R., Northfield, T.D., Paini, Dean R., Nyasani, Johnson O., Meyhöfer, R., Sevgan, Subramanian, Poehling, H-M., O'Donnell, Cheryle A., Olson, Stephen M., Riley, D., Scott, J.W., Parmenter, Kathy S., Thomas, J.E., Persley, Denis M., McGrath, D., Sharman, M., Walker, I.O, Peterson, Sophie, Macbeth, F.J., Reitz, Stuart R., Kennedy, George, Walgenbach, James, Powell Smith, J., Gitaitis, Ron, Moyer, James, Fonsah, Greg, Scott, Jay W., ‘Stormy’ Sparks, Alton, Abney, Mark, Fangston, David, Louws, Frank, Gunter, Christopher, Rugman-Jones, Paul F., Hoddle, M.S., Stouthamer, R., Scott-Brown, Alison, Veitch, Nigel C., Simmonds, Monique S.J., Schuetz, Ingeborg, Breitenstein, Antje, Moritz, Gerald, Mayamba, A., Muia, B., Sseruwagi, P., Ndunguru, J., Fred, F., Waiganjo, M., Abang, M.M., Montz, G.B., Sharma, Jyotsna, Funderburk, J., Chiemsombat, P., Campbell, P.R., Persley, D.M., Shock, Clinton C., Feibert, E.B.G., Saunders, L.D., Jensen, L.B., Mohan, S.K., Sampangi, R.K., Sims, Kelly, Reitz, S., Boucias, D., Sivalingam, P.N., More, T.A., Jain, R.K., Sivamani, Sundaraj, Krishnaveni, S., Usha, Z.B., Srinivasan, Rajagopalbab, Diffie, S., Mullis, S., Pappu, H.R., Sofue, Jun, Murai, Tamotsu, Sparks, Alton, Langston, D., Tang, Joe, Clover, G.R.G., Taylor, Matt, Terry, Irene, Walter, G., Moore, C., Roemer, R., Teulon, David A.J., Davidson, M.M., Perry, N.B., de Kogel, W.J., Free, Desley J., Turnia, Massimo, Tavella, L., Ullman, Diane, Verghese, Abraham, Harish, R., Watson, Andrew, Mo, J., Westmore, G., Allen, G.R., Davies, N.W., Wilson, Calum R., Poke, F.S., Wheeler, Greg S., Williams, D.A., Mound, F.A., Wilson, A.J., Yeh, Wen-Bin, Tseng, Mei-Zung, Lial, Ym-Yin, Chan, Niann-Tai, Tsai, Yi-Sheng, Zhang, Z-k, Zhang, Zhi-Jun, Wu, Q.J., Zhang, Y.J., and Lu, Y.B.

Subjects
Article
Published
2010

14. Olive seed wasps (Hymenoptera: Chalcidoidea), potentially important pests of olive crops in the Western Cape of South Africa

Author

Virgilio Caleca, Christian Giacalone, Elleunorah Allsopp, Carlo Costa, Maria Concetta Rizzo, Gabriella Lo Verde, Francesco Tortorici, Stefania Laudonia, Martina Sinno, Barbara van Asch, Gary Gibson, Caleca, Virgilio, Giacalone, Christian, Allsopp, Elleunorah, Costa, Carlo, Concetta Rizzo, Maria, Lo Verde, Gabriella, Tortorici, Francesco, Laudonia, Stefania, Sinno, Martina, van Asch, Barbara, and Gibson, Gary

Subjects
Eupelmus spermophilus, Olea europaea cuspidata, Eurytoma varicolor, Bactrocera oleae, Eupelmidae
Abstract

Most phytophagous insects on cultivated olives, Olea europaea L. subsp. europaea var. europaea, in South Africa are native and come from the local wild olive tree, Olea europaea subsp. cuspidata. This paper presents the first qualitative and comparative data regarding the phytophagous seed wasps on cultivated and wild olives. From 2009 to 2012, 62 random fruit samples were collected from untreated cultivated olive trees and 53 random fruit samples from wild olives at 13 different localities in the Western Cape. During spring 2013 and summer 2014, olives collected from the trees were dissected with a scalpel (when the pit was still soft) in search of seed wasp eggs and young larvae or cut with a pipe cutter (when the pit was hard) in search of mature larvae and pupae. Collected young and mature larvae were sequenced for the barcoding COI region (650 bp), and compared with adult seed wasp reference sequences. Seed wasp infestation was clearly higher in cultivated olives than Bactrocera oleae infestation (14% vs. 8%), reaching a peak of 66% in cultivar Koroneiki. Our results, including field data obtained from cultivated olives for the first time, demonstrate that Eupelmus spermophilus is phytophagous and in the Western Cape is virtually the only seed wasp in cultivated olives (99% in cultivated olives, 81% in wild ones). A precise assessment of the economic impact of seed wasps is being done by calculating the induced fruit drop, studying the susceptibility of olive cultivars and the influence of wild olives near olive crops.

Published
2019

15. Wild olive seed weevil in South Africa, Anchonocranus oleae Marshall (Coleoptera Curculionidae), a rediscovery after a century

Author

Virgilio Caleca, Christian Giacalone, Elleunorah Allsopp, Carlo Costa, Francesco Tortorici, Stefania Laudonia, Barbara van Asch, Enzo Colonnelli, Caleca, Virgilio, Giacalone, Christian, Allsopp, Elleunorah, Costa, Carlo, Tortorici, Francesco, Laudonia, Stefania, van Asch, Barbara, Colonnelli, Enzo, and Virgilio Caleca, Christian Giacalone, Elleunorah Allsopp, Carlo Costa, Francesco Tortorici, Stefania Laudonia, Barbara van Asch, Enzo Colonnelli

Subjects
Olea europaea cuspidata, Western Cape, Eastern Cape, olive seed wasps, classical biological control, Settore AGR/11 - Entomologia Generale E Applicata
Abstract

The wild olive seed weevil, Anchonocranus oleae Marshall, 1912 was not found after its description and the study of its biology by Silvestri in 1915. Recently, A. oleae larvae were found alive inside the kernels of wild olives (O. europaea subsp. cuspidata) collected from trees between 2009 and 2012 in the Western Cape, South Africa. No weevil larvae were found in cultivated olives collected during the same period. Only two adult weevils emerged from olives collected on the tree and on the ground. Over the sampling period 20 adults were collected directly on wild olive trees at different sites in the Western Cape and one in the Eastern Cape. All adults were shown to be conspecific with the holotype of A. oleae preserved in the Natural History Museum, London. Females and males have fully developed metathoracic wings, although they were never seen flying in limited field and laboratory observations. Adults in the laboratory immediately fed on wild olive pulp, but refused to feed on cultivated olives, even in the absence of alternative food. Oviposition sites remain visible even when the fruit pulp has dried out. The extremely low rate of adult emergence from wild olives can explain its absence in records of recent surveys. Barcoding COI sequences of young and mature larvae matched reference sequences obtained from A. oleae adults. The clear preference of A. oleae for wild olive fruit and its extremely low occurrence in cultivated olives suggest its possible use in classical biological control programs for invasive wild olive.

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results