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Arsenic is known to have detrimental effects on living bodies when exposed to contaminated groundwater. Therefore, a cost-effective way to eliminate arsenic from aquatic sources is essential. Our study evaluated the efficacy of eight different types of easily accessible agricultural wastes for arsenic removal. The safety assessment of the post-adsorbed solution in plant and animal test models such as Allium cepa and Daphnia magna was also evaluated. The results showed that the efficiency of the adsorbents depends on the type of agricultural waste utilized. In the case of citrus agrowastes, maximum biosorption (94–95%) was attained at the lowest 15 min contact time, indicating very rapid saturation of adsorption sites for other adsorbents; with an increase of contact time, there was a gradual increase in biosorption of arsenic. Allium cepa toxicity test showed that arsenic exposure caused a significant decrease in root length (2.80 ± 0.89 cm) and a reduction in mitotic index (6.31 ± 1.79%) that can be reverted to normal after the roots were grown in a post-adsorbed medium. Similarly, solutions treated with different agricultural wastes except citrus biosorbents showed significantly higher survival rates and litter sizes of Daphnia magna post biosorption. Agricultural waste with citric characteristics removed arsenic more effectively than other chosen agricultural wastes, but the solution generated by biosorption with citric agrowaste does not support plant and animal growth. Among the other chosen adsorbents, rice husk has been found to be highly suitable for plant and animal growth by reducing the toxic effects of arsenic. [ABSTRACT FROM AUTHOR]

Onion and garlic are economically important vegetable crops cultivated worldwide. Numerous pests and diseases affect the quality and yield of these crops. In addition to diseases and pests, several physiological disorders affect onion and garlic. The physiological disorders are abnormalities caused by intercultural operations, nutrient management, environmental factors, genetic regulation, etc. These physiological disorders significantly affect the yield and quality of onion and garlic, leading to monetary losses to the farmers. The following physiological disorders are commonly reported in onion: premature bolting, sprouting in storage, watery scale, doubling/twins, basal plate split, and thick neck. Premature sprouting and rubberization are the main physiological anomalies observed in garlic. The present review described the symptoms of these physiological abnormalities, the factors responsible, and ways to avoid or minimise the occurrence of these abnormalities to subsequently reduce the losses of the growers. Further, we also performed bibliometric analysis using the SCOPUS database. This is the first review that describes the progress of research on physiological disorders in onion and garlic in detail, which will positively increase awareness about such important aspects of onion and garlic. Further, it will provide insight to researchers for developing innovative strategies, cultural practices, and varieties to control these physiological abnormalities of onion and garlic. [ABSTRACT FROM AUTHOR]

Onion (Allium cepa L.) is an important seasoning vegetable worldwide. It belongs to the Allium genus, which produces distinctive flavor compounds, allicin/isoallicin. It has been known that allicin/isoallicin is produced upon cell damage by vacuole-localized alliinase releasing. Pungent isoallicin and lachrymaroty factor (LF) are unique features of onions. To understand the isoallicin system of onions, we identified and characterized the biosynthesis-related genes by displaying transcriptional profiles and analyzing the isoallicin contents of onion plants. The DHW30006 onion genome encoded 64 alliinase (ALL) and 29 LF synthase (LFS) proteins, which are the key enzymes for producing of isoallicin and LF. Interestingly, when we analyzed the N-terminal signal peptide sequences (SP) necessary for transport to the vacuole, we found that 14 ALLs contained the SP (SP-ALL) and 50 ALLs did not (non-SP-ALL). We hypothesized that non-SP-ALLs stayed in the cytosol of onion cells, reacted with isoalliin, and generated isoallicin without cell damage. Our transcriptome and LC-MS/MS analyses reveal that isoallicin levels vary significantly across onion tissues and growth stages, with substantial production occurring in intact cells through cytosolic alliinases and an increase through vacuolar alliinases upon tissue disruption. This novel observation suggests that the isoallicin system in onions functions as a dual-defense mechanism: cytosolic alliinases maintain a constant level of defense against biotic stress in undamaged tissues, while vacuolar alliinases enhance isoallicin production in response to tissue damage by herbivory and insects. Together, these coordinated mechanisms demonstrate an adaptable and dynamic defense strategy against biotic stresses in Allium plants. [ABSTRACT FROM AUTHOR]

The bulb onion (Allium cepa) is second only to tomatoes in value of vegetable crops cultivated worldwide. Water-soaked lesions, bleaching and blight on leaves and slippery skin, sour skin, soft rot near the neck and centre rot on onion bulbs were observed in surveys of the onion fields, storage as well as fruit and vegetable markets of northwestern provinces of Iran in 2020. Thirty-four samples were collected from onion fields of East Azerbaijan, West Azerbaijan and Zanjan provinces and the central storage of Bonab city. Fifty bacterial strains were isolated from symptomatic samples. Pathogenicity of the strains was confirmed by inoculating on onion bulb cultivars Azarshahr and Ghermez Anari. Twenty-eight strains were able to induce soft rot on the inoculated sites of cut onion bulbs. Onion bulbs turned dark brown at lesion sites and became odorous over time. All pathogenic strains were Gram-negative, facultative aerobic and catalase positive. Strains mainly varied in starch and aesculin hydrolysis, potato rot and hypersensitive reaction. Pathogenic strains separated into four groups based on phenotypic assays. In the phylogenetic tree based on concatenated sequences of gyrB and infB genes, the strains were divided into four clusters containing the type strains of Enterobacter ludwigii, Klebsiella variicola, Proteus mirabilis and Pantoea agglomerans, confirming their identity as belonging to these four species. This is the first report of P. mirabilis as a plant pathogen and of K. variicola as a causal agent of onion bulb rot. Moreover, this is the first report of E. ludwigii and P. agglomerans associated with onion bulb rot in Iran. [ABSTRACT FROM AUTHOR]

Onions (Allium cepa L.) are a globally significant horticultural crop, ranking second only to tomatoes in terms of cultivation and consumption. However, due to the crop's complex genome structure, lengthy growth cycle, self-incompatibility, and susceptibility to disease, onion breeding is challenging. To address these issues, we implemented digital breeding techniques utilizing genomic data from 98 elite onion lines. We identified 51,499 high-quality variants and employed these data to construct a genomic estimated breeding value (GEBV) model and apply machine learning methods for bulb weight prediction. Validation with 260 new individuals revealed that the machine learning model achieved an accuracy of 83.2% and required only thirty-nine SNPs. Subsequent in silico crossbreeding simulations indicated that offspring from the top 5% of elite lines exhibited the highest bulb weights, aligning with traditional phenotypic selection methods. This approach demonstrates that early-stage selection based on genotypic information followed by crossbreeding can achieve economically viable breeding results. This methodology is not restricted to bulb weight and can be applied to various horticultural traits, significantly improving the efficiency of onion breeding through advanced digital technologies. The integration of genomic data, machine learning, and computer simulations provides a powerful framework for data-driven breeding strategies, accelerating the development of superior onion varieties to meet global demand. [ABSTRACT FROM AUTHOR]

Plants are sensitive to environmental pollutants and are excellent organisms for genetic and physiological testing. Plant-based test systems are often used to study aquatic, aerial, and terrestrial pollution, especially Allium cepa, but studies with Tradescatia pallida specimens have gained prominence due to their sensitivity and applicability. Among the biomarkers, cytogenetic damage and chlorophyll levels are used in stress studies due to their responses to single or combined factors. The aim of this study was to evaluate cytogenetic and photosynthetic responses in T. pallida, and cytogenetic responses in A. cepa exposed to water from three sampling stations in the Juara lagoon (Municipality of Serra, ES, Brazil), collected in two sampling campaigns. The cytotoxic, genotoxic, and mutagenic potentials were analyzed using the T. pallida root tip mitosis assay and the Allium cepa test. Chloroplast pigment levels were measured in T. pallida leaves after chronic exposure to the lagoon water. The cytogenetic tests showed cytogenetic alterations at two sampling stations in at least one sampling campaign, suggesting the presence of potential pollutants, the effects of which were maximized during the rainy season. The study of photosynthetic metabolism in T. pallida showed a relationship between the levels of chloroplast pigments and the amount of nutrients present in the water. [ABSTRACT FROM AUTHOR]

Excess phosphorus (P) loading has been identified as an important cause of poor water quality in Lake Simcoe. A small (∼4%) but important source of P is the marshland that was developed for agriculture in and near the Holland Marsh. Applying the optimum rate of P fertilizer is an economical and environmentally friendly approach to crop management. In Ontario, P fertilizer recommendations are made based on the Olsen P soil test that is accredited by the Ontario Ministry of Agriculture, Food and Rural Affairs (OMAFRA). The current study was a response to concern about high application rates of P fertilizer and grower and industry suggestions that the recommended rates for P were too low. The marketable yield of yellow cooking onions was assessed in relation to different rates of P fertilizer in high organic matter (muck) soils. Organic matter ranged from 38% to 86%. Small- (6 site-years) and large-scale (21 site-years) field trials were conducted in the Holland Marsh area from 2009 to 2011 on muck soils that varied in pre-plant P (17–312 mg L−1). The study demonstrated that there was more plant-available P in the soil than was required for optimum yield in most fields; thus, P fertilizer applications had no effect on marketable yield of the onions. These results supported the OMAFRA P recommendations for onion production on muck soils, especially for soil test P levels over 61 mg L−1 where a response to applied P is rated as "low, rare, or no response". [ABSTRACT FROM AUTHOR]

Featured Application: Low-cost method for the assessment of the presence of cytotoxic and genotoxic contaminants in water. This study employed the Allium cepa bioassay to evaluate the toxic effects of contaminants in the Yucatan aquifer. Seven monitoring wells were studied during September and October 2021. Nutrient concentrations showed significant variation between sites, with samples closer to the coast (P3 and P7) presenting higher ammonia and phosphate concentrations. The pesticides found at the highest concentration were δ-HCH and chlorpyrifos, with 141.44 and 175.92 ng/L, respectively. Heptachlor and aldrin were present in sites P4oct and P2sept. Interestingly, DDT values were highly correlated with caffeine concentrations. The PAHs acenaphthylene and the sum of B(k)fluoranthene and B(b)fluoranthene presented the highest prevalence. B(k)fluoranthene and B(b)fluoranthene were the PAHs found at the highest concentration. The results of the A. cepa bioassay indicated no nuclear abnormalities. The study also found no statistical differences in the mitotic index, root length, biomarkers of oxidative stress, and inhibition of B-esterases between sites and controls. In summary, the wells sampled in the present study had low concentrations of contaminants that can be used as a proxy of anthropogenic discharges; the lack of effect in the biomarkers used at organism, cellular, and biochemical levels indicated no toxic effect on A. cepa roots. [ABSTRACT FROM AUTHOR]

The improvement of onion (Allium cepa L.) depends on the characterization and evaluation of the germplasm to find superior genotypes with desired horticultural qualities and utilization of diversity in future breeding. The study aimed to evaluate advanced breeding lines (consisting of both red- and white-skinned types) through genetic component analyses and genetic divergence using multivariate analysis to identify genotypes that can be utilized in breeding for table and dehydration purposes, respectively. Genotypes ‘(EM-AVT-2) REC-21-48,’ ‘(RIET) RVA-21-01,’ and ‘(EM-AVT-2) REC-21-46’ were found to be high yielders. Higher estimates of the genotypic coefficient of variation, heritability and genetic advance for the percentage of the double bulb, total soluble solid, and reducing sugar contents indicate additive gene action, and selection based on these parameters would be more reliable. The plant height, polar diameter, equatorial diameter, and neck thickness emerged as important selection indices. Principal components such as the plant height, number of leaves per plant, percentage of bolting and double bulb had values of >1, accounting for 81.73% of the total variation. Based on mean values and multivariate analysis, onion genotypes ‘(EM-AVT-2) REC-21-46’ (red-skinned), ‘(EM-AVT-2) REC-21-52’ (red-skinned), ‘(WIET) WVA-21-47’ (white-skinned), ‘(WAVT-2) WVC-21-74’ (white-skinned), ‘(WIET) WVA-21-39’ (white-skinned) and ‘(WHTSS-AVT-2) WTC-21-84’ (white-skinned) could be utilized as potential genitors in future breeding for table and dehydration purpose. [ABSTRACT FROM AUTHOR]

Background: Allium cepa is one of the major vegetable crops cultivated in Saudi Arabia. This economically important crop faces several biotic stresses which adversely affect the quality and quantity of its production. Several virus groups (potyviruses, carlaviruses, tospoviruses, and allexiviruses) have been reported infecting Allium crops. Methods: During the growing season of 2021-2022, a total of 81 onion samples exhibiting virus-like symptoms were collected from two different geographical regions in Saudi Arabia. The serological technique (ELISA) was used to detect the important allexiviruses and potyviruses. RT-PCR amplification of partial genome sequence was done using degenerate primers for allexiviruses and potyviruses and the phylogenetic trees were constructed using different bioinformatic tools. Results: The results obtained from ELISA tests showed that 26% and 32% of onion samples were positive with both Garlic Virus A (GarV-A) and Onion yellow dwarf virus (OYDV) respectively. RT-PCR amplification and sequencing results showed that two allexiviruses, GarV-A, garlic virus D (GarV-D), and one Potyvirus (OYDV) were detected in both regions. Sequence data were deposited in the GenBank database with accession numbers, OQ397545, OQ397546 for GarV-A, OQ397547 for GarV-D, and OQ397548, OQ397549 for OYDV, sequentially. Phylogenetic tree analysis of these virus isolates showed making clades with closely related isolates of their respective viruses. Pairwise nucleotide sequence identity showed their similarity with GarVA, GarV-D, and OYDV isolates reported earlier in the GenBank. Conclusion: To the best of our knowledge, these two distinct allexiviruses (GarV-A, GarV-D) and one Potyvirus (OYDV) were isolated for the first time from an onion crop in Saudi Arabia. [ABSTRACT FROM AUTHOR]

O manejo fitossanitário da cultura da cebola tem como principais agentes bióticos na fase de desenvolvimento vegetativo e formação de bulbos o míldio, Peronospora destructor (Berk.) Casp. (Peronosporales: Peronosporaceae), e o tripes, Thrips tabaci Lindeman 1888 (Thysanoptera: Thripidae). O objetivo deste estudo foi avaliar altas diluições dinamizadas de cloridrato de fenazopiridina e cartilagem de tubarão no manejo de tripes, míldio, produtividade e pós-colheita de cebola produzida em sistema orgânico. Os experimentos foram conduzidos a campo na Estação Experimental de Ituporanga, Epagri, SC, entre agosto a novembro de 2019 e 2020. O estudo foi composto por dois experimentos separados, sendo utilizado em cada unidade experimental os preparados em altas diluições dinamizadas de fenazopiridina e cartilagem de tubarão, nas potências 6CH, 12CH e 30CH (CH, ordem de diluição centesimal hahnemanniana) e testemunha sem aplicação. O delineamento experimental foi o de blocos ao acaso com cinco repetições. Altas diluições dinamizadas de fenazopiridina apresentaram redução de 15,4% da severidade de míldio com menor nível para 12CH no ano de 2020. A incidência e danos de tripes, produtividade e rendimento pós-colheita não foram influenciados pelos tratamentos.

Abstract Salinity stress negatively affects the growth and yield of crops worldwide. Onion (Allium cepa L.) is moderately sensitive to salinity. Beneficial microorganisms can potentially confer salinity tolerance. This study investigated the effects of endomycorrhizal fungi (M), Pseudomonas putida (Ps) and their combination (MPs) on onion growth under control (0 ppm), moderate (2000 ppm) and high (4000 ppm) NaCl salinity levels. A pot experiment was conducted with sandy loam soil and onion cultivar Giza 20. Results showed that salinity reduced growth attributes, leaf pigments, biomass and bulb yield while increasing oxidative stress markers. However, individual or combined inoculations significantly increased plant height, bulb diameter and biomass production compared to uninoculated plants under saline conditions. MPs treatment provided the highest stimulation, followed by Pseudomonas and mycorrhizae alone. Overall, dual microbial inoculation showed synergistic interaction, conferring maximum benefits for onion growth, bulbing through integrated physiological and biochemical processes under salinity. Bulb yield showed 3.5, 36 and 83% increase over control at 0, 2000 and 4000 ppm salinity, respectively. In conclusion, combined application of mycorrhizal-Pseudomonas inoculations (MPs) effectively mitigate salinity stress. This approach serves as a promising biotechnology for ensuring sustainable onion productivity under saline conditions.

Copper oxide (CuO) and zinc oxide (ZnO) nanoparticles were synthesized by Pterocarpus santalinus leaf extract following the green method as a reducing and capping agent. Analytical methods like scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and UV-visible spectroscopy were followed for characterization of synthesized nanoparticles. Outcomes demonstrated that crystalline CuO and ZnO nanoparticles with distinct morphologies have successfully formed. The ability of metal oxide nanomaterials against the growth of Aspergillus niger fungus was tested by agar diffusion method on PDA plates. Pure culture of the isolated fungus from onions was used in this experiment. Fungal inoculum was prepared and spread on PDA plates and tested for growth inhibition with varying concentrations of CuO and ZnO nanoparticles using Disk Diffusion Method. A successful growth inhibition was identified in plates treated with CuO and ZnO.

[Objective] The study aims to explore the antibacterial activity and allelopathy of flavonoids from leaves of Eleutherococcus brachypus, and to provide the basis for the development of plant-derived antimicrobial agents and the application of their allelopathy in plant protection and agricultural production. [Methods] Leaf flavonoids was extracted by an ultrasonic-assisted method from E. brachypus leaves, then the antibacterial sphere, the minimum inhibitory concentration (MIC), the apoptosis rate, MDA content, ROS content, and the activity of antioxidant enzymes of epidermal cells from onion scaly leaves, as well as the inhibitory rate of the onion root and shoot growth were determined with different concentrations of leaf flavonoid extracts (100%, 50%, and 25%). [Results] (1) The yield of leaf flavonoids from E. brachypus was 42.3 mg/g. (2) At same concentration, the diameter of the inhibition zone was S. aureus > B. subtilis > Salmonella, respectively, and expanded with the increase in the extract concentrations with the same strain. The minimum inhibitory concentration of the three strains was also S. aureus < B. subtilis < Salmonella, respectively. (3) Each concentration of leaf flavonoids from E. brachypus induced apoptosis in the upper epidermal cells of Allium cepa. Meanwhile, as the concentration of flavonoid extracts increased, the apoptosis rate, MDA content, and H2O2 content were increased. POD activity and CAT activity were increased first and then decreased during the same time of treatment, which were higher in treatment groups than the control. (4) The growth of root and shoot of the onion scale leaves were inhibited under the treatment of flavonoids extracts, which was negatively correlated with the treatment dose. [Conclusion] The antibacterial effects of the leaf flavonoid extracts of E. brachypus on Gram-positive bacteria are better than that of Gram-negative bacteria, and the antibacterial effects are positively correlated with the concentration of flavonoid extracts. Moreover, all these changes suggest that the allelopathic mechanism of flavonoid extracts from E. brachypus may be due to the overproduction of ROS and the inhibition of antioxidant enzyme activity, which cause oxidative damage to membrane lipids and cell death.

The Allium cepa assay is a valuable tool for evaluating natural products as potential anticancer agents by monitoring mitosis progression in onion root tips. However, the traditional method of identifying and classifying cells into various mitotic stages, and categorizing them as either dividing or non-dividing cells to calculate the mitotic index, can be laborious and prone to mistakes. This study aims to streamline the process of computing for the mitotic index using a convolutional neural network to identify and classify cells into different mitotic stages, and categorize them as dividing and non-dividing cells. A dataset of 1548 high-quality images of onion root tip cells displaying different stages of mitosis was used. A pretrained YOLOv8 model achieved an 88.33% mean average precision and 83% recall. Experiment results show that this approach can reduce the time-consuming aspects of the assay and potentially accelerate laboratory experiments performed in the search for novel anticancer drugs. [ABSTRACT FROM AUTHOR]

During the 2022 growing season, commercial crops of onion (Allium cepa cv. Medusa) were found to be affected by an extensive leaf blight in south western Slovakia. The pathogen present on diseased leaves was isolated and identified as Stemphylium vesicarium through morphological and molecular analyses, utilizing three DNA markers (ITS, tef1-α, and β-tubulin). A detailed description and illustration of the fungus are provided. Although this pathogen is already present in Slovakia, this is the first record of S. vesicarium causing leaf blight on A. cepa in the country. Pathogenicity tests with a fungal isolate were performed on three local cultivars of onion plants in the laboratory, indicating varying susceptibility of the cultivars to S. vesicarium. [ABSTRACT FROM AUTHOR]

Evolution of genus is accompanied by ecological diversification. The majority of species grow in open, sunny, rather dry sites in arid and moderately humid climates. However, Allium species have adapted for many other ecological niches. Classical approaches for the identification of Allium cultivars are based on morphological traits. The assessment of these traits is difficult and their evaluation can be subjective considering that most of these cultivars are closely related. Hence, this study of Internal Transcribed Spacer (ITS) sequencing and four barcoding regions, matK, rbcL, trnH-psbA, trnL and Inter Simple Sequence Repeats (ISSR) were researched in onion, Allium cepa L. (Alliaceae) collected from three different cultivation sites. The results established noticeable hereditary divergence among the three cultivars. In ITS and matK, BDUT 1453, BDUT 1454 and BDUT 1455 were independent of each other and formed three clusters. In rbcL, BDUT 1453 formed an independent cluster from the cluster of BDUT 1454 and BDUT 1455. But in trnH-psbA, BDUT 1454 formed an independent cluster and BDUT 1453 and BDUT 1455 were closely placed whereas trnL showed all the three forming a cluster wherein BDUT 1453 and BDUT 1454 were placed closely in a sub-cluster. In ISSR, BDUT 1454 and BDUT 1455 formed a single cluster and BDUT 1453 diverged from it. Even though the tested cultivars belong to the same species they showed genetic divergence among themselves. [ABSTRACT FROM AUTHOR]

The mobile crystalline material-41 (MCM-41) has been used in various fields, such as measuring cations, dye removal, and drug delivery. Modification of MCM-41 improves some properties of mesoporous materials, including surface area, adsorption capacity, thermal stability, and mechanical stability. Considering of these, after synthesize of MCM-41@SiO2-NH-pydc, in vivo and in vitro studies of MCM-41@SiO2-NH-pydc were conducted. For in vitro toxicity, various concentrations of 75, 150, and 300 mg L−1 were used in Allium cepa test to study the toxicity and genotoxicity of mesoporous. For in vivo toxicity, 40 Wistar albino rats were collected and acute toxicity was estimated through oral administration (PO). For the sub-chronic study, four groups of Wistar rats were randomly selected and received MCM-41@SiO2-NH-pydc daily by gavage at dosages of 12.5, 25, and 50 mg kg−1 for 28 days. For hematological and biochemical tests, blood was collected, and the liver and kidney were dissected and prepared as slides to study histopathology. The lethal dose 50 (LD50) of MCM-41@SiO2-NH-pydc was found to be more than 250 mg kg−1. All hematological and biochemical parameters were reported as normal, indicating no serious toxicity. Therefore, based on the findings obtained, it is suggested to use MCM-41@SiO2-NH-pydc as a safe, appropriate, and novel carrier for drug delivery investigations. [ABSTRACT FROM AUTHOR]

Acne is a common skin condition that affects people of all ages and can lead to significant physical and psychological distress. The first line of action against acne is topical products, though the most effective are topical antibiotics. In such a scenario, the development of effective and safe herbal formulations for the treatment of acne is of great importance. Rubia cordifolia, Aloe barbadensis, and Allium cepa extracts are rich sources of bioactive metabolites and are safe compared with antibiotics, in addition to being cost effective, sustainable, and eco-friendly. Also, their combination has not been studied for treating acne, and their potential benefits need to be investigated. The present study aimed to develop an effective polyherbal gel formulation of R. cordifolia, A. barbadensis, and A. cepa combined extract for treating acne and validate its effect with reference to conventional antibiotics. Plant materials were extracted in water by the reflux method, and phytochemical analysis was done for flavonoid, anthraquinone, and phenolic contents. The combined extract (R. cordifolia, A. barbadensis, and A. cepa extracts) was formulated in gel. The selected polyherbal gel was evaluated for in vitro antibacterial activity using agar well diffusion against Cutibacterium acnes (P. acnes) culture. Phytochemical analysis of the composite extract revealed the rich presence of flavonoids, phenolics, and anthraquinones. The polyherbal gels showed good physicochemical properties; however, FCEG-4 was selected for further studies. It was found to be effective against C. acnes (MTCC 1951) in agar well diffusion, as it showed a similar zone of inhibition as that of standard. Also, during in vivo studies, FCEG-4 showed comparable efficacy with clindamycin gel. It was concluded from the study that composite extracts incorporated in an aqueous-based gel system were effective in topical therapy of mild acne vulgaris, showing similar efficacy to that of clindamycin cream. [ABSTRACT FROM AUTHOR]

Salinity stress negatively affects the growth and yield of crops worldwide. Onion (Allium cepa L.) is moderately sensitive to salinity. Beneficial microorganisms can potentially confer salinity tolerance. This study investigated the effects of endomycorrhizal fungi (M), Pseudomonas putida (Ps) and their combination (MPs) on onion growth under control (0 ppm), moderate (2000 ppm) and high (4000 ppm) NaCl salinity levels. A pot experiment was conducted with sandy loam soil and onion cultivar Giza 20. Results showed that salinity reduced growth attributes, leaf pigments, biomass and bulb yield while increasing oxidative stress markers. However, individual or combined inoculations significantly increased plant height, bulb diameter and biomass production compared to uninoculated plants under saline conditions. MPs treatment provided the highest stimulation, followed by Pseudomonas and mycorrhizae alone. Overall, dual microbial inoculation showed synergistic interaction, conferring maximum benefits for onion growth, bulbing through integrated physiological and biochemical processes under salinity. Bulb yield showed 3.5, 36 and 83% increase over control at 0, 2000 and 4000 ppm salinity, respectively. In conclusion, combined application of mycorrhizal-Pseudomonas inoculations (MPs) effectively mitigate salinity stress. This approach serves as a promising biotechnology for ensuring sustainable onion productivity under saline conditions. [ABSTRACT FROM AUTHOR]

Copper oxide (CuO) and zinc oxide (ZnO) nanoparticles were synthesized by Pterocarpus santalinus leaf extract following the green method as a reducing and capping agent. Analytical methods like scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and UV-visible spectroscopy were followed for characterization of synthesized nanoparticles. Outcomes demonstrated that crystalline CuO and ZnO nanoparticles with distinct morphologies have successfully formed. The ability of metal oxide nanomaterials against the growth of Aspergillus niger fungus was tested by agar diffusion method on PDA plates. Pure culture of the isolated fungus from onions was used in this experiment. Fungal inoculum was prepared and spread on PDA plates and tested for growth inhibition with varying concentrations of CuO and ZnO nanoparticles using Disk Diffusion Method. A successful growth inhibition was identified in plates treated with CuO and ZnO. [ABSTRACT FROM AUTHOR]

Purpose. Practice of medicinal plants for the treatment of various ailments is a history for several decades. Occurrence of active compounds in these plants makes it significant in the era of modern medicine. Garlic and onion a monocotyledonous bulbous flowering plant belonging to the genus Allium has an extensive tradition for being used as anti-inflammatory, anti-cancer, anti-hyperepidemic, antidiabetic, anti-bacterial, neuroprotective agent against various disorders. Modern day synthetic formulations have reduced efficacy and rendered with side effects that channelled the exploration for alternate source in plants for dealing with various disorders. The present study aims to explore the bioactive compounds present in Allium sativum (Garlic) and Allium cepa (Red Onion). Methods. Garlic and onion powder was soaked in Methanol, filtered, and concentrated in a vacuum in a rotary evaporator. Fractionation was carried out.LC-MS analysis of aqueous fraction was performed using a Waters Mass Q-TOF Mass Spectrometer with diode array detector. Results. LC-MS analysis revealed the occurrence of 200 compounds in garlic and 184 compounds in onion. 3.53% in garlic and 4.37% in onion were the major chemical constituents based on the number of hits. Conclusion. This study suggests that the Allium sativum and Allium cepa herbal powders being loaded with large number of active compounds would be promising alternative agents due to their richness in antioxidants. Clin Ter 2024; 175 (5):274-286 doi: 10.7417/ CT.2024.5131 [ABSTRACT FROM AUTHOR]

The olive tree is crucial to the Mediterranean agricultural economy but faces significant threats from climate change and soil-borne pathogens like Verticillium dahliae. This study assesses the dual role of an onion extract formulation, rich in organosulfur compounds, as both biostimulant and antifungal agent. Research was conducted across three settings: a controlled climatic chamber with non-stressed olive trees; an experimental farm with olive trees under abiotic stress; and two commercial olive orchards affected by V. dahliae. Results showed that in the climatic chamber, onion extract significantly reduced MDA levels in olive leaves, with a more pronounced reduction observed when the extract was applied by irrigation compared to foliar spray. The treatment also increased root length by up to 37.1% compared to controls. In field trials, irrigation with onion extract increased the number of new shoots by 148% and the length of shoots by 53.5%. In commercial orchards, treated trees exhibited reduced MDA levels, lower V. dahliae density, and a 26.7% increase in fruit fat content. These findings suggest that the onion extract effectively reduces oxidative stress and pathogen colonization, while enhancing plant development and fruit fat content. This supports the use of the onion extract formulation as a promising, sustainable alternative to chemical treatments for improving olive crop resilience. [ABSTRACT FROM AUTHOR]

Onion thrips, Thrips tabaci Lind. (Thysanoptera: Thripidae) is a major pest of onion worldwide. It damages onion, Allium cepa L., both directly and indirectly, which leads to significant output losses. In 2022, research was conducted in a private onion field in the Delta region, Egypt, to evaluate the efficacy of bioinsecticides and synthetic insecticides and to evaluate the efficacy of macro- and micronutrient in determining treatment effects on onion thrips populations. Injury caused by onion thrips is often not severe enough to warrant the application of numerous pesticides to crops in the Delta region of Egypt. Spinosad (Tracer 24% SC) and spinetoram (Radiant 12% SC) were used as bioinsecticides, and Pyridalyl (Pleo 50% EC) was used as a synthetic insecticide for managing onion thrips populations. The greatest decrease in infestation was achieved by the application of the chemical insecticide (Pyridalyl) Pleo 50% EC and the bioinsecticide (Spinosad) Tracer 24% SC. The greatest decrease in infestation was achieved by the application of the micronutrient boron. [ABSTRACT FROM AUTHOR]

Approximately 40–50% of municipal solid waste is organic and causing biogenic malodor and infections, due to inefficient treatment methods. Biorefinery-based bioremediation and valorization is in vogue against these conventional strategies since it combines unit operations for better efficiency and productivity. Deriving inspiration, the proposed strategy puts together a unique and compatible combination of processes. This novel two-step valorization workflow involves the extraction of small molecules using organic solvents, and fermentation of resulting denatured residues (increased biodegradability or decreased recalcitrance) of reduced microbial load. The extraction step also doubles up as a sterilization event, with different solvents (petroleum ether, chloroform, ethyl methyl ketone and methanol) exhibiting varied efficiency, methanol and ethyl methyl ketone being the most effective. Different recalcitrant plant organic wastes resulting from four plants (Cocos nucifera, Allium cepa, Artocarpus hirsutus and Swietenia mahagoni) were used as feedstocks in the preliminary exploratory study using chosen pathogenic bacteria. Onion peel (Allium cepa) ethyl methyl ketone extract was chosen for further studies, as it inhibits Salmonella enterica, which is associated with infection and malodour (due to biogenic H2S) in wastewater. Further, fractionation of the extract yielded quercetin and its glycoside. The onion peel residue, after solvent extraction was fortified with peptone and essential minerals to promote the growth of Bacillus clausii. Fortified post-extraction residue supported the growth better than the pre-extraction residue. The residue resultant after solvent extraction was fermented with Bacillus clausii and with release of bioactive supernatants. The concentrated supernatant showed significant inhibition of Salmonella enterica and Shigella dysenteriae. Additionally, all the exudates showed considerable inhibition in H2S production, respectively.

Onion and garlic are economically important vegetable crops cultivated worldwide. Numerous pests and diseases affect the quality and yield of these crops. In addition to diseases and pests, several physiological disorders affect onion and garlic. The physiological disorders are abnormalities caused by intercultural operations, nutrient management, environmental factors, genetic regulation, etc. These physiological disorders significantly affect the yield and quality of onion and garlic, leading to monetary losses to the farmers. The following physiological disorders are commonly reported in onion: premature bolting, sprouting in storage, watery scale, doubling/twins, basal plate split, and thick neck. Premature sprouting and rubberization are the main physiological anomalies observed in garlic. The present review described the symptoms of these physiological abnormalities, the factors responsible, and ways to avoid or minimise the occurrence of these abnormalities to subsequently reduce the losses of the growers. Further, we also performed bibliometric analysis using the SCOPUS database. This is the first review that describes the progress of research on physiological disorders in onion and garlic in detail, which will positively increase awareness about such important aspects of onion and garlic. Further, it will provide insight to researchers for developing innovative strategies, cultural practices, and varieties to control these physiological abnormalities of onion and garlic.

Onion (Allium cepa L.) is an important seasoning vegetable worldwide. It belongs to the Allium genus, which produces distinctive flavor compounds, allicin/isoallicin. It has been known that allicin/isoallicin is produced upon cell damage by vacuole-localized alliinase releasing. Pungent isoallicin and lachrymaroty factor (LF) are unique features of onions. To understand the isoallicin system of onions, we identified and characterized the biosynthesis-related genes by displaying transcriptional profiles and analyzing the isoallicin contents of onion plants. The DHW30006 onion genome encoded 64 alliinase (ALL) and 29 LF synthase (LFS) proteins, which are the key enzymes for producing of isoallicin and LF. Interestingly, when we analyzed the N-terminal signal peptide sequences (SP) necessary for transport to the vacuole, we found that 14 ALLs contained the SP (SP-ALL) and 50 ALLs did not (non-SP-ALL). We hypothesized that non-SP-ALLs stayed in the cytosol of onion cells, reacted with isoalliin, and generated isoallicin without cell damage. Our transcriptome and LC-MS/MS analyses reveal that isoallicin levels vary significantly across onion tissues and growth stages, with substantial production occurring in intact cells through cytosolic alliinases and an increase through vacuolar alliinases upon tissue disruption. This novel observation suggests that the isoallicin system in onions functions as a dual-defense mechanism: cytosolic alliinases maintain a constant level of defense against biotic stress in undamaged tissues, while vacuolar alliinases enhance isoallicin production in response to tissue damage by herbivory and insects. Together, these coordinated mechanisms demonstrate an adaptable and dynamic defense strategy against biotic stresses in Allium plants.

Organic production is driven by global demand, necessitating the exploration and implementation of novel agricultural approaches. To enhance this approach, many natural resources are available. Thus, this study aims to evaluate the effects of biochar (BiCh) and organic fertilizer (OrF) to improve the nutrient quality and productivity of onion (Allium cepa) plants. The consumption of fresh onion plants offers numerous health benefits due to their rich nutrient content. This study investigates the impact of BiCh (0, 4, and 8 ton/ha) and OrF (0, 10, and 20 L/ha) on the enhancement of root volume, morphology, yield, and biochemical attributes of onion plants. OrF at 10 L/ha significantly influences leaf weight (16.00 g), antioxidants in the bulb (4.22 μg/g), nitrogen in the leaf (2.63%), phosphorus in the leaf (0.31%), and bulbs (0.26%). Moreover, combining BiCh at 4 ton/ha with OrF at 10 L/ha positively affects most characteristics. Applying BiCh at 4 ton/ha did not impact the plant. In contrast, the control group exhibited the lowest values in key parameters, emphasizing the importance of BiCh and OrF applications in enhancing onion plant performance. These findings provide practical insights for farmers aiming to optimize organic production and contribute to economically sustainable practices in high tunnel environments.

The current study investigates how genotype by environment interaction (GEI) complicates superior cultivar selection in onion breeding. Employing a randomized block design with three replications, we assessed GEI effects on onion yield and identified adaptable, stable genotypes. The sensitivity of onion genotypes to environmental changes significantly influences yield and quality, necessitating adaptability across diverse climatic conditions. We conducted multilocation trials evaluating 28 onion genotypes across four distinct locations, each representing varying environmental factors. Through phylogenetic analysis, genotypes were categorized into four clusters: cluster I (3), cluster II (4), cluster III (15), and cluster IV (6). Rigorous assessment of yield performance, employing additive main effects and multiplicative interaction models, particularly the AMMI model, revealed five genotypes demonstrating remarkable stability and potential across diverse environmental conditions: RO-1626, RO-1623, RO-1639, RO-1625, and RO-1627. Notably, genotypes from cluster II exhibited the highest marketable yield (277.06 q/ha) and total yield (295.66 q/ha), indicating adaptability to varied environmental conditions. These findings hold promise for breeding high-yielding onion varieties resilient to diverse environments, ensuring stability, adaptability, and quality in cultivation.

Heliotropium keralense Sivar. & Manilal is an endangered medicinal plant native to the Indian state of Kerala. Cytotoxic effects of aqueous extracts of leaves, stems, and roots of H. keralense were evaluated using Allium cepa L. root tip method. Allium cepa bulbs were exposed to extracts of different parts of the plant for 24 hours. Compared to the negative control, a significant decrease in the length, root number and mitotic index of Allium cepa was observed with 5 to 25% aqueous extracts of H. keralense. Chromosomal abnormalities such as single, double, and multiple lesions in interphase, single and double lesions in prophase, diagonal metaphase, diagonal anaphase, bridged anaphase, strap-shaped nuclei, giant cells and chromosome loops are identified in positive control and treatments. The highest percentage of chromosomal aberration was observed in the (95.18±2.07%) positive control and 25% (71.76±7.46%) leaf extract. The analysis showed that the aqueous plant parts of H. keralense had antimitotic and cytotoxic effects. This study shows that Heliotropium keralense contains strong cytotoxic substances that can cause chromosomal aberrations.

Heat stress (HS) is a major physiological stressor that induces oxidative damage, inflammation, and metabolic disruptions, all of which cause harm to health. This study investigates the potential protective effects of Allium cepa L. (AC), against the physiological alterations brought on by HS. Twenty male rats were utilized in this study and divided into 4 groups: Control, HS, AC, and HS + AC. Rats were exposed to 38–39 °C for 2 h each day for three weeks to induce HS while 1.0 ml/100 g body weight of ethanolic extract of AC was administered orally for three weeks. Hematological parameters, inflammatory markers, and lipid profiles were analyzed using blood samples obtained through heart puncture.The findings demonstrated that HS significantly lowered levels of hemoglobin (HB), red blood cell (RBC) counts, and antioxidant enzyme activity. However, there was a significant rise in the levels of inflammatory markers, platelet counts, LDL-c triglyceride (TG), total cholesterol (TC), and white blood cell (WBC). Many of these alterations were reversed by AC supplementation, by increasing RBC counts, HB levels, and antioxidant enzyme activities while decreasing LDL-c, TG and TC, MDA, and TNF-α (tumor necrosis factor-α). However, the positive benefits of AC were partially diminished in the HS + AC group, perhaps due to the severe oxidative stress caused by heat stress (HS).This study highlights the probable potential of AC as a natural antioxidant in modifying heat stress-induced oxidative damage, hematological changes, and lipid metabolism disruptions; however, its protective effects are insufficient to mitigate heat stress. Therefore, further research is required to explore the other possible underlying mechanisms.

This paper aimed the evaluation of weed control in onion crop regarding spray tips and herbicide tank mixture. It was conducted in a commercial area in Imbuia municipality, in SC, Brazil. Randomized block experimental design was used, with treatments organized in factorial scheme (2 x 4) + 2, and four repetition. The treatments consisted in two spraying tips models: TT 110 015 and ADIA 110 02, and four herbicide mixtures: ioxynil + flumioxazin (IO + FL; 250 + 75 g i.a. ha-1 ), ioxynil + pendimethalin (IO + PE; 250 + 1.200 g i.a. ha-1 ), diuron + flumioxazin (DI + FL; 500 + 75 g i.a. ha-1 ) e diuron + pendimethalin (DI + PE; 500 + 1.200 g i.a. ha-1 ). Additionally, two controls, with and without deweeding were kept. Assessed variables were Amaranthus deflexus, Sonchus oleraceus, Polygonum persicaria and Coronopus didymus control; phytotoxicity, plant stand, bulb's diameter and average weight and, commercial and total yield. All treatments were effective controlling A. deflexus, S. oleraceus and P. persicaria. C. didymus controls were lower than 81% for DI + PE treatment applied with ADIA 110 02 spraying tip. The phytotoxicity was higher than 18% and 8 % at 7 and 14 Days After Spraying (DAS) respectively, involving herbicides IO + FL and DI + FL. Spraying tips and herbicide tank mixtures did not influence yield components or bulb productivity. All tank mixtures were efficient to controlling weed plants, and selective for onion crop, regardless the spraying tip. [ABSTRACT FROM AUTHOR]

The purpose of the experiment was to investigate onion (Allium cepa) root growth, cell division, and mitotic index. The onions were placed into four different water samples (Control water, Azadi bridge, Sar Razan, and Sar Baskan) for 72 hours in beakers. The results showed that the number of divided cells decreased significantly in the sewage water compared with the control water. The control water had a high percentage of divided cells (18%), whereas the Sar Razan sewage water had the least percentage of divided cells (1.3%). The percentage of divided cells in Sar Baskan sewage water was 2%, and the percentage of divided cells in Azadi Bridge was 2.6%. It was found that the mitotic index and the number of divided cells increased in the control water compared to the sewage water. The results showed that sewage water has a greater impact on root growth, cell division, and mitotic index of root tips of A. cepa. [ABSTRACT FROM AUTHOR]

The aims of this study were: to analyze the sustainability of shallot farming in Jeneponto and to analyze the sensitivity attributes of shallot farming sustainability in Jeneponto. Shallot farming sustainability analysis was performed using the Multi-Dimensional Scaling (MDS) approach. Based on analysis results, the overall sustainability status of onion farming in Jeneponto is fairly sustainable (54.68%) accumulated from the fairly sustainable score (64.31%, 52.96%, 55.18%) in social, economy, and technology dimensions respectively while on the contrary, less sustainable score (46.26%) was observed from the institution dimension. The results of the study suggested that the leverage attributes which is sensitive to shallot farming sustainability encompass: 1) labor availability; 2) farmer-to-farmer interaction; 3) industrial availability; 4) use of superior variety; 5) use of agricultural machinery; 6) organic fertilization; and 7) post-harvest processing. These leverage attributes must be prioritized by the stakeholders through the planning and implementation of appropriate policies to maintain shallot farming sustainability in Jeneponto. [ABSTRACT FROM AUTHOR]

The wastes from onion usually litter sales outlets in various markets across the country. Onion (Allium cepa) peel wastes (OPW) was assessed for its chemical content, nutritional and organoleptic quality on smoked catfish (Clarias gariepinus) stored for a duration of 8 weeks at room temperature. The OPW at concentrations of 0g, 2.5g and 5g (T0, T1 and T2 respectively) were sprinkled on the fish samples before and after smoking. Samples of OPW and fish were analyzed chemically for proximate composition, and determination of organoleptic quality according to standard method by panel of 20 on a 9-point hedonic scale. Nitrogen free extract (43.26%) constituted the highest analyte of the OPW. This was followed by ash (32.95%); crude fibre (11.45%); moisture content (6.69%); crude protein (4.40%) and ether extract (1.25%). The composition of fish preserved with OPW was significantly different when compared with the initial and final carcass analysis of the control group. The analysis revealed crude protein in T0 (49.84%), T1 (36.91%), T2 (43.13%); ash in T0 (7.70%), T1(14.05%), T2 (8.20%) while ether extract T0 (8.65), T1 (5.55%) and T2 (7.76%). In terms of organoleptic assessment, all the mean values of the fish samples were above 5 which indicated that they were not disliked. The control fish, T0 (0.0g/0.5kg) had superior taste, 7.95%; appearance, 7.60%; aroma, 7.45%; texture, 7.60% and acceptability, 7.85%. this was followed by T2 (5.0g/0.5kg) with no significant variation (p < 0.05) among the test samples. Inference from the study showed that OPW at 5g can be used to preserve smoked fish without change in quality, sensory and organoleptic assessment for 8 weeks. [ABSTRACT FROM AUTHOR]

Rapid leaching of soluble nitrogen (N) sources in soil poses a significant challenge in agricultural practices. Therefore, gaining a comprehensive understanding of crop responses to slow-release N application rates has become crucial to contributing valuable insights to optimize N management strategies in agriculture. A field study was conducted to investigate the influence of preplant calcium cyanamide fertilizer on the growth, yield, quality, and shelf life of short-day onion. Six levels of calcium cyanamide (CaCN2, 19.8% N), 0, 90, 120, 200, 400, and 600 kg.ha-1 CaCN2, which are equivalent to 0, 17.82, 23.76, 39.6, 79.2, and 118.8 kg.ha-1 N, respectively, replicated four times were broadcasted and incorporated into the top 5 to 10 cm of soil. Using 400 kg.ha-1 of CaCN2 yielded noteworthy improvements in various parameters of onion growth, such as plant height, leaf count, bulb weight per plant, bulb diameter, bulb length, and overall plant weight, as indicated by the study results. The application of different levels of CaCN2 as an N source exerted a significant influence on these growth factors. Moreover, the study revealed a direct correlation between CaCN2 application levels and the storage life of onions. Specifically, the findings demonstrated that the application of 400 kg.ha-1 CaCN2 resulted in enhanced yield and overall onion plant growth. However, the application of 600 kg.ha-1 CaCN2 increased the incidences of bulb weight loss, rots, and sprouting during the 8-week storage period at room temperature. These findings provide valuable insights for onion investors and farmers in the region and offer practical recommendations for optimizing fertilizer use and storage practices to improve onion production and minimize postharvest losses. [ABSTRACT FROM AUTHOR]

Downy mildew is a production limiting factor for Brazil's main onion producing region. In the present study we tested two management strategies, namely the utilization of a resistant cultivar and the implementation of a disease alert system. Our objective was to mitigate the frequency of systemic fungicide applications while concurrently sustaining optimal yield levels. By carrying out fungicide sprays only when the alert system indicated a severe risk and using the cultivar "SCS379 Robusta", it was possible to reduce the number of sprays from 11 (calendar) to 5 (alert system), maintaining statistically similar yields (32.58 tonnes/ha – calendar; and 30.76 tonnes/ha – alert system). The reduction in spray applications using an integrated management strategy reduced the cost of spraying by more than half from BRL 0.069/Kg to BRL 0.033/Kg. This study demonstrates that implementation of an integrated management strategy can reduce chemical use and cost without compromising the protection of onion using alert system and more susceptible varieties. [ABSTRACT FROM AUTHOR]

The hidden hunger for Se in the world population is well known. As a strategy to address this issue, this study aimed to biofortify onion (Allium cepa L.), since it is a condiment vegetable consumed by different economic classes and is widely used in the food industry. To this end, Se doses (0, 10, 25, 50, 100 and 200 g ha-1) and application forms (soil and foliar) were evaluated on the biofortification of the bulb and crop yield. The Se source was sodium selenate. Via soil, Se was applied together with NPK fertilizer in the pretransplantation of the seedlings. Via foliar application, Se was sprayed 77 d after seedlings were transplanted. The Se increased yield up to 99 g ha-1, which was 14.7% higher than the yield obtained in the crop not fertilized with Se. Foliar application of Se was more efficient than soil application in the biofortification of onion, although both were effective. With the dose that promoted the highest yield, the Se concentrations reached 0.487 and 0.317 mg Se kg-1 in onion dry mass, when the supply was made via foliar and soil applications, respectively. The increase in Se supply did not influence bulb quality, as demonstrated by the macro-and micronutrients content and pungency. [ABSTRACT FROM AUTHOR]

Background: Rabeprazole (RPZ), a widely used proton pump inhibitor, is known to have toxic effects on human beings. Objective: To evaluate the toxic effects of RPZ sodium (RPZ-Na) using plant-based eukaryotic test systems. Methods: The toxic effect of RPZ-Na (0.025-0.4 mM) was evaluated on Allium cepa, Allium sativum, and Cicer arietinum at different exposure times using CuSO4 as a reference standard. Results: RPZ-Na concentration-dependently reduced the root length of A. cepa and A. sativum, as well as the shoot and root lengths of C. arietinum. RPZ-Na at 0.1 to 0.4 µg/mL and at 48 h exposure time exerted toxic effects on the tested systems. Conclusions: RPZ-Na exerted a concentration- and time-dependent toxic effect on A. cepa, A. sativum, and C. arietinum. Therefore, it is important to take adequate precautions during its long-term use. [ABSTRACT FROM AUTHOR]

Pink root disease (PRD) caused by Setophoma terrestris is a major disease in onion‐producing regions. The fungus primarily infects onion, but occasionally causes disease on other plant species such as cereals, corn, cucurbits, pepper, spinach, and soybean. In this study, we analysed the efficacy of three commercially available fungicides at low dose in two successive years (2020–2021) in the field. These included Priaxor Xemium Brand (fluxapyroxad + pyraclostrobin) at 1.0 or 1.5 L/ha, Lamardor 400 FS (propiconazole + tebuconazole) at 2.5 kg/ha, and Rovral TS (iprodione) at 5.0 kg/ha applied via soil surface irrigation, and also Priaxor as foliar application (1.0 L/ha) against PRD on three onion cultivars: white, red, and yellow. All fungicides were applied thrice within 3‐week intervals from the onset of PRD (mid‐February), and fungicidal efficacy was determined 2 weeks prior to harvest (mid‐May). The lowest PRD severity was recorded for Priaxor 1.5 L/ha at 9.5%, followed by Lamardo at 10.1%, and the remaining treatments compared with the untreated control at 47.7% severity. The highest production (per 100 bulbs) was recorded for Priaxor 1.5 L/ha with 7.36 kg, followed by Lamardo with 6.86 kg and the remaining treatments compared with untreated at 4.33 kg. When treatment effects on soil enzymes were examined prior to harvest, there was no substantial change in urease activity, whereas soil application of Priaxor increased alkaline phosphatase activity by 50% and decreased acidic phosphatase by 42% compared with the untreated control. Our data indicated that fungicides have subtle differences in efficacy profiles, which may translate to improved management against PRD. [ABSTRACT FROM AUTHOR]

Gold nanoparticles (Au NPs) have potentially therapeutic properties as they are synthesized via biomolecules as reducing and stabilizing agent(s). The aim of this study is to develop an easy and eco-friendly method for the synthesis of Au NPs using extracts from the Allium cepa (AC) red peel (skin) extract and investigate the antibacterial and antioxidant activity and also inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. UV–Vis peak at ~ 564 nm confirmed the Au NPs absorbance. TEM images revealed the formation of Au NPs with mostly spherical shapes and sizes between 6.08 and 54.20 nm. FTIR analysis confirmed the important biological compounds responsible for the reduction of gold. The strong absorption property of Au NPs was studied by EDX. The produced Au NPs demonstrated significant antibacterial and antifungal activity against the bacterial and fungal strains tested, as well as efficient inhibitory activity against both AChE and BChE enzymes. The highest antimicrobial activities were found against Staphylococcus aureus (0.06° mg/ml) and Candida albicans (0.06° mg/ml). The antioxidant test findings revealed that AC-Au NPs had lesser activity when compared to normal antioxidants. The Au NPs showed excellent inhibitory efficacy against AChE and BChE. The proposed green technique could encourage the innocuous generation of Au NPs, implying therapeutic possibilities. [ABSTRACT FROM AUTHOR]

Among the compounds present in necro-leachate, a liquid released during the process of decomposition of the human body, are the biogenic amines cadaverine and putrescine. Although some studies on necro-leachate have indicated a potential ecotoxicological and public health risk associated with it, the research on this type of contamination is still rather limited. This study presents information about the phytotoxic and cytogenotoxic potential of cadaverine and putrescine, evaluated separately and within a mixture. Phytotoxicity was evaluated through a germination test, the initial growth of seedlings with Lactuca sativa, and cytogenotoxicity through chromosomal aberration and micronucleus tests with Allium cepa. The L. sativa results showed a phytotoxic effect for the evaluated amines, by reducing root (> 90%) and hypocotyl (> 80%) elongation. The co-exposure of cadaverine and putrescine potentiated cytogenotoxic activity by aneugenic action in the meristematic cells of A. cepa. From this result, it is possible to infer the eco-toxicogenic potential of cadaverine and putrescine. This study not only highlights the importance of the phytotoxic and cytogenotoxic effects of these amines but also emphasizes the urgent need for further investigation into contamination originating from cemetery environments. By evaluating the risks associated with necro-leachate, this research is aimed at informing global efforts to protect ecological and public health. [ABSTRACT FROM AUTHOR]

The genus Achyranthes belong to the family Amaranthaceae which constitutes an important group of herbs and shrubs with immense medicinal value. The present research work was conducted to investigate the anticancer potential of Achyranthes aspera L. leaves by focusing on the antioxidant, aniproliferative and antimitotic activities of leaf extracts. Plant extraction was carried out by soxhelt method with different solvents. Phytochemical characterization of the plants extracts using chemical methods identified the presence of cardiac glycosides, saponins, coumarins, proteins, tannins, flavonoids and triterpenes. Alkaloid was present in methanolic and ethanolic extract. High performance liquid chromatography showed presence of different concentration of myricetin, quercetin and kaempferol in different extracts with the highest concentration of myricetin (84.53 μg/mL) in n‐butanolic extract. The extracts were then tested for antioxidant activity using 2,2‐diphenylpicrylhydrazyl (DPPH) radical scavenging assay by spectrophotometric method. In DPPH radical scavenging assay, antioxidant activity of A. aspera ranged between 79.78 ± 0.034% and 58.63 ± 0.069%. Highest antioxidant activity was observed for methanolic extract and lowest for acetone. Antimitotic activity was determined by using Allium cepa assay in which microscopic investigation was carried out to observe normal and abnormal phases of mitosis. In this assay, n‐butanolic extract had highest antimitotic activity with minimum mitotic index at 2 mg/mL (57 ± 0.0351%). The plant extracts also caused chromosomal and mitotic aberrations which were clearly observed under 40× and 100× magnification of compound microscope. Antiproliferative activity was determined by using yeast cell model in which light microscope with hemocytometer was used for cell counting. In case of Antiproliferative activity, the ethyl acetate extract of A. aspera had highest antiproliferative activity with lowest cell viability (22.14 ± 0.076%) at highest extract concentration (2 mg/mL) while methanol extract of A. aspera had highest antiproliferative activity with lower cell viability (24.24 ± 0.057%) at lowest extract concentration (0.25 mg/mL). The results of the study indicated that the leaves extract of A. aspera have strong potential to be used as a source of anti‐cancer agent. Research Highlights: Achyranthes aspera L. leaves have various phytochemicals which contribute to its medicinal propertiesVarious extracts of the leaves of A. aspera L. possess antioxidant, antimitotic and antiproliferative potentialThe results of the study indicated that the leaves extract of A. aspera have strong potential to be used as a source of anti‐cancer agent. [ABSTRACT FROM AUTHOR]

O estudo teve por objetivo verificar a incidência de tripes em maiores densidades populacionais submetidas a doses de nitrogênio e seus possíveis efeitos na produção de biomassa dos bulbos de cebola. Os tratamentos foram quatro densidades de plantas (400, 600, 800 mil plantas ha-1 e 1 milhão de plantas ha-1) submetidas a duas doses de nitrogênio (150 e 200 kg N ha-1). As temperaturas mais elevadas no desenvolvimento inicial e ausência de chuvas do final de agosto ao final de setembro propiciaram de forma precoce a incidência de tripes acima do nível de dano econômico. A incidência de tripes é reduzida de forma linear com o aumento da densidade de plantas, não sendo influenciada por doses de até 200 kg N ha-1. O uso de doses de nitrogênio acima de 150 kg N ha-1, mesmo em populações de um milhão de plantas por hectare, não aumenta a biomassa dos bulbos de cebola. Densidades populacionais a partir de 600 mil plantas ha-1 reduzem a biomassa fresca e seca dos bulbos de cebola.