Search

This article highlights an ongoing academic-community partnership between university researchers and City Year Miami, the local site of a national education non-profit serving the nation's third-largest school district. AmeriCorps Members (ACMs) serve as small-group interventionists and behavior/attendance coaches for the county's lowest performing students. Collaboration with City Year Miami supplemented their routine workforce support with trainings (n = 18) for City Year Miami Team Leaders (TLs) and ACMs focused on youth mental health. Trainings emphasized the Cognitive Triangle by highlighting how to bring compassion and intentionality to their work with students, school partners (e.g., teachers, teammates, and administrators), and their own self-care. We present our collaboration, the training model, and process data representing three layers of organizational voice that informed iterative revisions and refinement to the training model. Data sources (n = 45 TLs and ACMs) highlight what was learned from each group (TLs, ACMs, and leadership) and include: (1) pre-training survey data, (2) training-generated data such as attendance and exit slips, (3) post-training survey data measuring intent to use training content, and facilitators and barriers to use, and (4) meeting-generated data from formal (planned, agenda-driven) and informal (impromptu) partner discussions. Emphasis is placed on the role of City Year Miami organizational leaders and providers at all stages of research and implementation, as well as lessons learned in this community-partnered, school-engaged work, including takeaways related to positionality, partnership, and research.

The aim of this study is to determine how students believe their learning-related experiences (i.e., attention, affect, and time perception) have changed over the course of the pandemic. This study documented students' (N[subscript analyzed] = 191) relative judgments of change between their "current" experiences (measured April 2022) and their remembered experiences from three different timepoints: (1) before the pandemic-related restrictions (before March 2020; pre-restriction); (2) immediately after the restrictions were implemented (spring 2020; early restriction); and (3) immediately after they were lifted (~ winter/spring 2022; post-restriction). This study also captured how students predicted their experiences would change in the future. Roughly 2 years after pandemic-related restrictions were introduced, students reported perceiving reductions in their attention, affect, and time-perception compared to their remembered pre- and early restriction learning-related experiences. They also reported perceived reductions in their attention and affect even as pandemic-related restrictions were beginning to lift, though these declines were slightly attenuated. Regarding the future, students were optimistic that their learning-related experiences would improve in the coming months. These findings can support the creation of future approaches targeting the improvement of attention, affect, and productivity in learning and performance-based environments.

Abstract Fossils preserving soft tissues and lightly biomineralized structures are essential for the reconstruction of past ecosystems and their evolution. Understanding fossilization processes, including decay and mineralisation, is crucial for accurately interpreting ancient morphologies. Here we investigate the decay of marine and freshwater shrimps deposited on the surface of three different clay beds. In experimental set ups containing kaolinite, cryogenic scanning electron microscopy shows a black film comprised of newly formed anhedral and cryptocrystalline aluminosilicates on marine shrimp cuticles, which stabilise the overall morphology. This is the first experimental evidence for the replication of arthropod lightly biomineralized structures in aluminosilicates shortly after death, while carcasses are not buried by sediments. The preservation of morphology through aluminosilicates could result in carcasses persisting on the seafloor for weeks without losing much external anatomical information. In this context, instantaneous burial capturing animals alive may not be a prerequisite for exceptional preservation as usually thought.

Objectives: Despite clinical relevance, commercially available molecular tools for accurate β-lactamase detection are limited. In this study, we evaluated the performance of the ARM-DⓇ Kit, β-Lactamase, a commercially available multiplex PCR assay designed to detect nine β-lactamase genes, including the five major plasmid-mediated carbapenemases, ESBL and AmpC genes circulating in the United States. Methods: A diverse collection of 113 Gram-negative isolates, including 42 with multiple β-lactamases genes, was selected from the U.S. Centers for Disease Control and Prevention (CDC) & Food and Drug Administration (FDA) Antimicrobial Resistance Isolate Bank, to represent the most frequently detected bacterial species carrying plasmid-mediated β-lactam resistance genes. Results: Results were compared with whole genome sequence data. Of 164 β-lactamase gene targets with 49 unique variants, all were detected correctly without any cross-reactivity. The sensitivity and specificity were 100% (164/164) and 99.9% (852/853), respectively. Conclusion: The ARM-DⓇ Kit, β-Lactamase detected a wide range of β-lactamase genotypes at a low upfront cost. The Streck assay represents a suitable, comprehensive tool for the detection of key β-lactamase resistance genes of public health concern in the United States.

Abstract The miniaturization of Global Positioning Systems (GPS) transmitters is providing insights into the ecology and management of migratory bird species at biologically‐relevant spatial scales. However, transmitters and their attachment methods could bias inferred behaviors, demographic rates, and resulting management decisions. We evaluated the effects of external harness‐style GPS transmitters by comparing direct harvest rates, winter dispersal distances, and subsequent harvest distributions of mallards (Anas platyrhynchos) equipped with GPS transmitters (n = 443) to a tarsal banded‐only control group (n = 1,123) captured in western Tennessee during winters 2019–2022. We found that transmitter‐marked mallards had similar harvest rates, winter dispersal distances, and harvest distributions as banded‐only mallards. Time between capture and harvest predicted dispersal distances but there was no effect of marker type. Specifically, the average time from capture to harvest was 30 (SE = 2) and 31 (SE = 3) days for banded‐only and transmitter‐marked mallards, respectively. Harvest rate ( h) was only 2.2% greater for transmitter‐marked mallards compared to banded‐only mallards overall, but GPS transmitters affected harvest susceptibility of juveniles ( ∆ h juv = 14.5%). Based on harvest rates and dispersal distances between transmitter‐marked and banded‐only cohorts, we suggest 7‐ to 10‐day data censoring periods may be warranted, especially for juveniles, as mallards acclimate to GPS transmitters. Overall, we concluded that modern harness‐style GPS transmitters provided reliable information of wintering mallard space use, movements, and harvest mortality and can be used to inform ecology and management of wintering mallards and other dabbling ducks. Future studies should evaluate effects of harness‐style GPS transmitters for other species and during different portions of dabbling ducks' life cycle, such as migration or nesting, when they may experience greater adverse effects.

Cotton is an important agricultural crop to many regions across the globe but is sensitive to low-temperature exposure. The activity of the enzyme SENSITIVE TO FREEZING 2 (SFR2) improves cold tolerance of plants and produces trigalactosylsyldiacylglycerol (TGDG), but its role in cold sensitive plants, such as cotton remains unknown. Recently, it was reported that cotton SFR2 produced very little TGDG under normal and cold conditions. Here, we investigate cotton SFR2 activation and TGDG production. Using multiple approaches in the native system and transformation into Arabidopsis thaliana, as well as heterologous yeast expression, we provide evidence that cotton SFR2 activates differently than previously found among other plant species. We conclude with the hypothesis that SFR2 in cotton is not activated in a similar manner regarding acidification or freezing like Arabidopsis and that other regions of SFR2 protein are critical for activation of the enzyme than previously reported.

Quiescence is a reversible cell cycle exit traditionally thought to be associated with a metabolically inactive state. Recent work in muscle cells indicates that metabolic reprogramming is associated with quiescence. Whether metabolic changes occur in cancer to drive quiescence is unclear. Using a multi-omics approach, we found that the metabolic enzyme ACSS2, which converts acetate into acetyl-CoA, is both highly upregulated in quiescent ovarian cancer cells and required for their survival. Indeed, quiescent ovarian cancer cells have increased levels of acetate-derived acetyl-CoA, confirming increased ACSS2 activity in these cells. Furthermore, either inducing ACSS2 expression or supplementing cells with acetate was sufficient to induce a reversible quiescent cell cycle exit. RNA-Seq of acetate treated cells confirmed negative enrichment in multiple cell cycle pathways as well as enrichment of genes in a published G0 gene signature. Finally, analysis of patient data showed that ACSS2 expression is upregulated in tumor cells from ascites, which are thought to be more quiescent, compared to matched primary tumors. Additionally, high ACSS2 expression is associated with platinum resistance and worse outcomes. Together, this study points to a previously unrecognized ACSS2-mediated metabolic reprogramming that drives quiescence in ovarian cancer. As chemotherapies to treat ovarian cancer, such as platinum, have increased efficacy in highly proliferative cells, our data give rise to the intriguing question that metabolically-driven quiescence may affect therapeutic response.

The immunopathogenesis of psoriasis, a common chronic inflammatory disease of the skin, is incompletely understood. Here we demonstrate, using a combination of single cell and spatial RNA sequencing, IL-36 dependent amplification of IL-17A and TNF inflammatory responses in the absence of neutrophil proteases, which primarily occur within the supraspinous layer of the psoriatic epidermis. We further show that a subset of SFRP2+ fibroblasts in psoriasis contribute to amplification of the immune network through transition to a pro-inflammatory state. The SFRP2+ fibroblast communication network involves production of CCL13, CCL19 and CXCL12, connected by ligand-receptor interactions to other spatially proximate cell types: CCR2+ myeloid cells, CCR7+ LAMP3+ dendritic cells, and CXCR4 expressed on both CD8+ Tc17 cells and keratinocytes, respectively. The SFRP2+ fibroblasts also express cathepsin S, further amplifying inflammatory responses by activating IL-36G in keratinocytes. These data provide an in-depth view of psoriasis pathogenesis, which expands our understanding of the critical cellular participants to include inflammatory fibroblasts and their cellular interactions.

Qualitative accounts indicate there are sensory and communication related barriers to adequate childbirth and postnatal healthcare for autistic people. However, little quantitative work has explored the topic. This online survey study explored childbirth and postnatal experiences among 384 autistic and 492 non-autistic people. Compared with non-autistic people, autistic people were more likely to find the sensory aspects of birth overwhelming, and experienced lower satisfaction with birth-related and postnatal healthcare. Autistic people were more likely to experience postnatal depression and anxiety. The findings highlight that sensory and communication adjustments should be made to birth and postnatal healthcare for autistic people. The findings indicate the need for greater autism understanding among professionals and greater postnatal mental health support for autistic people.

Public administration educators in the United States have renewed their interest in incorporating an international perspective into their teaching. Why is this perspective important for students and how can educators integrate it meaningfully? In this article, I provide an argument for the internationalization of public administration curricula, generate nine "principles of practice" to help guide internationalization efforts, and specify two broad strategies through which an international dimension can be integrated into public administration curricula--one for leveraging comparative material in domestically-focused curricula and another for developing a distinct and standalone internationally-focused specialization.