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Horses (Equus ferus caballus) have evolved over the past 300 years in response to man-made selection for particular athletic traits. Some of the selected traits were selected based on the size and horses' muscular power (eg Clydesdales), whereas other breeds were bred for peak running performance (eg Thoroughbred and Arabian). Although the physiological changes and some of the cellular adaptations responsible for athletic potential of horses have been identified, the molecular mechanisms are only just beginning to be comprehensively investigated. The purpose of this review was to outline and discuss the current understanding of the molecular mechanisms underpinning the athletic performance and cardiorespiratory fitness in athletic breeds of horses. A brief review of the biology of epigenetics is provided, including discussion on DNA methylation, histone modifications and small RNAs, followed by a summary and critical review of the current work on the exercise-induced epigenetic and transcriptional changes in horses. Important unanswered questions and currently unexplored areas that deserve attention are highlighted. Finally, a rationale for the analysis of epigenetic modifications in the context with exercise-related traits and ailments associated with athletic breeds of horses is outlined in order to help guide future research.

Qiu, J, Huang, L, Davie, AJ, and Zhou, S. Comparison of the effects of endurance training on alternate days and on consecutive 4 days each week for 8 weeks on the abundance of PGC-1α, CaMKII, NRF-1, mtTFA, and COXIV proteins in rat skeletal muscle. J Strength Cond Res 33(11): 3136-3144, 2019-The aim of this study was to compare the effects of 2 training protocols, training on alternate days (A) or on consecutive 4 days followed by 3 days of rest in each week (C) for 8 weeks, on selected proteins involved in the biogenesis and function of mitochondria in skeletal muscle. Eighty male Sprague Dawley rats were randomly allocated into 10 groups (n = 8 each), including Pre and Post control groups and A or C training groups with 8, 16, 24, and 32 training sessions, respectively. The vastus lateralis and soleus muscle samples were obtained 24 hours after the last training session, or at rest for the controls. The abundance of the proteins for PGC-1α, CaMKII, NRF-1, mtTFA, and COXIV was analyzed by Western blotting. Analysis of the results with 2-way ANOVA showed no significant effect and interaction (training protocol by duration) in abundance of the proteins by the 2 protocols. However, fold changes normalized to control showed significant increases COXIV of the soleus muscle at most time points in both A and C training as indicated by Kruskal-Wallis H tests. There were significant correlations found between the abundance of the measured proteins of the vastus lateralis. The findings suggest that the 2 training protocols with the same intensity and total volume of work would not make a significant difference in respect of the changes in the targeted proteins. Alternative regulatory factors and the responses in different types of muscles to the training programs need to be examined in future research.

This study investigated the effects of six weeks of normobaric hypoxic training on transcriptional expression of the genes associated with mitochondrial and glycolytic activities in Thoroughbred horses. Eight horses were divided into two groups of four. They completed an identical incremental, moderate intensity training program, except that one group trained in a hypoxic chamber with 15% oxygen for 30 min on alternate days except Sundays (HT), while the other group trained in normal air (NC). Prior to and post training, heart rate and blood lactate were measured during an incremental treadmill test. Muscle biopsy samples were taken prior to and 24 h post the training period for qPCR analysis of mRNA changes in VEGF, PPARγ, HIF-1α, PGC-1α, COX4, AK3, LDH, PFK, PKm and SOD-2. No significant differences between the HT and NC were detected by independent-samples t-test with Bonferroni correction for multiple comparisons (P>0.05) in relative changes of mRNA abundance. There were no significant differences between groups for heart rate and blood lactate during the treadmill test. The outcomes indicated that this hypoxia training program did not cause a significant variation in basal level expression of the selected mRNAs in Thoroughbreds as compared with normoxic training., Summary: This was one of the first studies that examined transcriptional and physiological responses of Thoroughbred racehorses to six weeks moderate intensity treadmill training in a hypoxia chamber with 15% oxygen.

Aims The aims of this study were to determine the effects of four weeks of intermittent exposure to a moderate hypoxia environment (15% oxygen), and compare with the effects of exercise in normoxia or hypoxia, on glucose homeostasis, insulin sensitivity, GLUT4 translocation, insulin receptor phosphorylation, Akt-dependent GSK3 phosphorylation and Akt activity in skeletal muscle of obese mice with type 2 diabetes. Methods C57BL/6J mice that developed type 2 diabetes with a high-fat-diet (55% fat) (fasting blood glucose, FBG = 13.9 ± 0.69 (SD) mmol/L) were randomly allocated into diabetic control (DC), rest in hypoxia (DH), exercise in normoxia (DE), and exercise in hypoxia (DHE) groups (n = 7, each), together with a normal-diet (4% fat) control group (NC, FBG = 9.1 ± 1.11 (SD) mmol/L). The exercise groups ran on a treadmill at intensities of 75–90% VO2max. The interventions were applied one hour per day, six days per week for four weeks. Venous blood samples were analysed for FBG, insulin (FBI) and insulin sensitivity (QUICKI) pre and post the intervention period. The quadriceps muscle samples were collected 72 hours post the last intervention session for analysis of GLUT4 translocation, insulin receptor phosphorylation, Akt expression and phosphorylated GSK3 fusion protein by western blot. Akt activity was determined by the ratio of the phosphorylated GSK3 fusion protein to the total Akt protein. Results The FBG of the DH, DE and DHE groups returned to normal level (FBG = 9.4 ± 1.50, 8.86 ± 0.94 and 9.0 ± 1.13 (SD) mmol/L for DH, DE and DHE respectively, P < 0.05), with improved insulin sensitivity compared to DC (P < 0.05), after the four weeks treatment, while the NC and DC showed no significant changes, as analysed by general linear model with repeated measures. All three interventions resulted in a significant increase of GLUT4 translocation to cell membrane compared to the DC group (P < 0.05). The DE and DH showed a similar level of insulin receptor phosphorylation compared with NC that was significantly lower than the DC (P < 0.05) post intervention. The DH and DHE groups showed a significantly higher Akt activity compared to the DE, DC and NC (P < 0.05) post intervention, as analysed by one-way ANOVA. Conclusions This study produced new evidence that intermittent exposure to mild hypoxia (0.15 FiO2) for four weeks resulted in normalisation of FBG, improvement in whole body insulin sensitivity, and a significant increase of GLUT4 translocation in the skeletal muscle, that were similar to the effects of exercise intervention during the same time period, in mice with diet-induced type 2 diabetes. However, exercise in hypoxia for four weeks did not have additive effects on these responses. The outcomes of the research may contribute to the development of effective, alternative and complementary interventions for management of hyperglycaemia and type 2 diabetes, particularly for individuals with limitations in participation of physical activity.

To understand the molecular mechanisms of adaptation to different training protocols, this study compared the effects of 4 sessions of 90 min treadmill exercise on alternate days or consecutive days in 1 week on messenger RNA (mRNA) and protein expression of proliferator-activated receptor-γ coactivator 1-α in rat gastrocnemius muscle. The mRNA significantly increased by 25.8-fold after alternate-day and 10.1-fold after consecutive-day training, while the protein showed no significant cumulative effect, 1.5–1.7-fold above baseline, in the 2 protocols.

This study investigated the effect of restricting nasal breathing during a series of 20-m shuttle runs. Ten male participants (mean age = 21.7 ± 2.4 years, height = 1.80 ± 0.62 m, mass = 79.2 ± 10.4 kg, sum of 4 skinfolds = 54.5 ± 7.8 mm) were required to either (a) dive on the ground and complete a rolling sequence (condition = GRD) or (b) complete the shuttles while staying on their feet and tagging the line with 1 foot, at the end of each 20-m segment (condition = STD). The shuttle runs were completed with and without a nose clip (no clip = nc; with a clip = clip) under 4 different trial conditions in a randomized order (GRDnc; GRDclip; STDnc; and STDclip), requiring the participants to return on 4 separate occasions separated by 5-7 days. Heart rate was recorded throughout each trial, and the rate of perceived exertion (RPE) was measured at the completion of each shuttle sequence. Pretrial and posttrial lactate and respiratory function measures were also recorded. The general linear model with repeated measures analysis indicated that there was a significant effect for Roll (GRD > STD) (p ≤ 0.05) but not for Clip (p > 0.05) on total time to completion in the trials. There was no significant interaction of the conditions (Roll × Clip) for RPE (p > 0.05). Similarly, there was no significant effect for blood lactate measured 3 minutes post the last shuttle for Roll (p > 0.05) and Clip (p > 0.05). There was a significant main effect on the HR across all 6 time points (i.e., pre, intervals 1-4 and 10 minutes post) (p ≤ 0.05) and for Roll (GRD > STD) (p ≤ 0.05), but not for Clip (p > 0.05). No significant effect of Roll or Clip was found for any of the recorded ventilation measures (p > 0.05). On the basis of these findings, the use of restricted nasal breathing, while performing a high-intensity shuttle sequence as a method of increasing the acute training effect on athletes, is questionable, so strength and conditioning coaches should carefully consider their rationale for using such a training strategy.

It is known that electromyostimulation (EMS) alone or superimposed over voluntary contraction (EV) can effectively improve muscle strength. However, the effect of this type of training on the ability to control force production at submaximal levels is unknown. The authors examined the effects of EV training on steadiness in force production of knee extensors and flexors in older adults. Forty participants, including 20 men and 20 women, 60-77 years of age, were randomly allocated into a control group (CG) and an electromyostimulation superimposed over voluntary contraction (EVG) group. The EVG performed 30 bilateral isometric knee extension and flexion contractions per session, 3 training sessions per week, for 6 weeks. The variations in force production, expressed in absolute (standard deviation [SD]) and relative (coefficient of variation [CV]) terms, were assessed in isometric contractions at 5%, 15% and 25% maximal voluntary contraction (MVC) levels. Results indicated that MVC increased in knee extension and flexion in EVG (p < .05) after the training; steadiness CV also improved at 15% MVC in knee flexion (p < .05) but no significant changes were found in knee extension and steadiness SD. The training-induced changes in MVC were not correlated to steadiness CV that might indicate different mechanisms underlying these adaptations.

Cycle tests for maximal oxygen uptake (VO2max) have traditionally used incremental resistance protocols (RP) at a constant cadence. The purpose of this study was to evaluate whether an incremental cadence protocol (CP) using a constant resistance relative to gross body mass was as reliable and valid in eliciting VO2max as RP in trained cyclists. Ten male recreational cyclists aged 25.2 ± 6.8 years completed two CP and one RP trials in a randomized order over a 3-week period. The CP started at a workload of 2.75 W per kg body mass, with the cadence increased by 10 rpm each minute from 70 rpm. The RP started from 125 W with workload increased 25 W each minute with a constant cadence of 90 rpm. The results showed no significant differences between the CP (mean of the two CP trials) and RP for peak VO2 (3.9 ± 0.6 vs. 4.0 ± 0.8 L·min−1), peak ventilation (140.5 ± 22.8 vs. 143.0 ± 27.1 L·min−1) and post-exercise blood lactate (11.4 ± 2.1 vs. 11.9 ± 1.6 mmol·L−1), while peak heart rate (183.9 ± 10.5 vs. 187.5 ± 11.4 beats·min−1) and peak workload (319.9 ± 60.2 vs. 375.1 ± 67.3 W) were significantly less for the CP than the RP. For the two CP trials, the intraclass correlation coefficient for test–retest reliability was 0.96, the technical error of measurement (TEM) was 0.17 L·min−1, and the relative TEM was 4.35%. The results indicate that the CP is equally effective in eliciting VO2max as the RP and is a reliable method of measuring VO2max in trained recreational cyclists.

Summary Relationships between racing performance and plasma lactate and uric acid concentrations after racing were investigated in pacing Standardbred racehorses. The study was undertaken in order to better understand factors that limit racing performance. Twenty horses were tested after races of 1760 m and 28 horses after races over 2160 m. Blood samples were taken 30–60 min before and 8 and 30 min after a race. There were no significant differences between the race distances for prerace and 8 min postrace plasma lactates. Significant low correlations were obtained for plasma lactate concentration 8 min postrace and the number of race wins (r = 0.29, P = 0.04), number of race placings (1st, 2nd or 3rd; r = 0.34, P = 0.02) and lifetime earnings (r = 0.29, P = 0.04). Net lactate was correlated with the number of race placings (r = 0.31, P = 0.03). There were no significant correlations between performance indices and plasma uric acid concentrations in races of 1760 m. For races over 2160 m, correlations were found between plasma uric acid concentration at 8 min postrace and the number of race wins (r = 0.37, P = 0.06, 95% confidence limits 0-0.65). As well, there was a significant correlation between uric acid concentration at 8 min postrace and lifetime earnings (r = 0.35, P = 0.07, 95% confidence limits 0.06-0.64). Only 10–15% of the variability in retrospective career performance in pacing Standardbreds can be explained by these metabolic markers of the muscle anaerobic response to racing. Plasma lactate and uric acid responses to maximal exercise are unlikely to be useful for evaluating racing performance in pacing Standardbreds in univariate analysis.

Introduction Resistance training (RT) has been shown to improve chronic heart failure (CHF) patients' functional ability and quality of life. Despite these benefits RT has not always been recommend as a form of exercise principally because of a concern for acceleration of the left ventricular (LV) remodeling process. This study investigated the effects of 8 weeks RT on the LV structure and function of patients with CHF. Method Fifteen men who suffered from CHF were divided into either a RT program or non-training control group. Before and after 8 weeks of training patients underwent resting echocardiography to assess their end-diastolic and end-systolic dimensions (EDD and ESD, respectively), ejection fraction (EF), fractional shortening (FS) and stroke volume (SV). Results A repeated measured ANOVA showed that 8 weeks of RT had no significant effect on the LV measurements (group×time, p >0.05). Post training comparison, however, revealed that the EF and FS of the training group was significantly higher than in the control group (40.9±10.5% vs. 30.3±4.6%, p =0.029 and 25.0±7.0% vs. 17.4±3.1%, p =0.020 respectively). Conclusion RT is a suitable method of training for CHF patients since it does not cause a reduction of LV contractility function or enhance myocardial deterioration as measured by EF and FS.

The purpose of this investigation was to identify characteristics of the muscle protein titin in different athletic populations with increased levels of strength and power relative to non-athletes. Subjects fell into one of four groups: (1) non-athletes (NA) ( n=5), (2) weightlifters (WL) (n=5), (3) powerlifters (PL) (n=5), (4) sprinters (S) (n=5). A one repetition maximum in the squat exercise was performed to assess strength. In addition, countermovement vertical jump trials were performed to assess power capabilities. Peak power (W(peak)) was calculated for the vertical jumps from force plate measurements. From gel electrophoresis analyses of muscle samples, titin-1 (T1) and titin-2 (T2) protein bands were identified, quantified and expressed relative to each other. In addition the relative mobility (R(f)) of T1 and T2 was determined as an estimate of molecular weight. The NA group [%T1=47.8 (5.1), %T2=52.2 (5.1), mean (SE)] had lower T1 and higher T2 percentages than WL [%T1=62.3 (6.6), %T2=37.7 (6.6)], PL [%T1=66.8 (5.0), %T2=33.2 (5.0)] and S [%T1=65.9 (4.9), %T2=34.1 (4.9)] groups (P< or =0.10, preliminary investigation into titin and exercise justifies more liberal alpha level). No significant differences were found in R(f) of T1 or T2 between the groups. This investigation has shown that there is a differential expression of titin protein bands in competitive athletes with increased levels of strength and power in comparison to untrained non-athletic individuals. Some relationships between titin characteristics and athletic performance were observed; however, no conclusions can be made based on these data as to the contribution of titin to strength or power capabilities.

Many studies have focused on identifying the genes or single nucleotide polymorphisms associated with the athletic ability of thoroughbreds, but few have considered differences in maternal and paternal heritability of athletic ability. Herein, we report on our association study of career race performances of 675 Australian thoroughbreds with their pedigrees. Racing performance data (prize money per start) were collected from the Bloodhound database. The performance of all horses was categorised as either poor or elite athletic achievement. Then, 675 foals were divided by their parents’ performance (elite or poor) into four groups: (1) elite dams and elite sires; (2) elite dams and poor sires; (3) poor dams and elite sires; and (4) poor dams and poor sires. The performance of foals was then compared between the four groups. The results show that the heritability of race performance between dams and foals (r = 0.141, P

The EMG power spectrum may shift towards higher frequencies with higher movement velocities. Fatigue, on the other hand, can cause a decrease in the frequency components. The purpose of this study was to examine acute effects of explosive (EE) and heavy resistance (HRE) concentric leg press exercise on muscle force, EMG and blood lactate. The EE included five sets of ten repetitions with 40+/-6% of the isometric maximum at a 100 degrees knee angle performed as explosively as possible. The same number of repetitions was performed in HRE but with a heavier load (67+/-7% of the isometric maximum at a 100 degrees knee angle). Maximal isometric and single concentric actions of different loads, and an isometric fatigue test were measured before and after both exercises. Surface EMG was recorded from the vastus medialis muscles for analyses of average EMG (aEMG) and EMG power spectrum. Muscle fiber composition of the vastus lateralis was determined and blood lactate measured throughout the exercises. Mean power frequency and median frequency were higher during EE than during HRE (P0.05). They increased during EE (P0.05) as the exercise progressed, whereas during HRE no change or even slight decreases were observed. Signs of fatigue after pure concentric work were not observed after EE, and even after HRE, possibly due to the relatively small range of motion and short duration of action time, the fatigue was not that extensive. The relative number of fast twitch fibers was correlated (r=0.87, P0.05) with the change in blood lactate in HRE. It was concluded that there may be a greater use of fast twitch motor units in explosive movements and that instead of fatigue, the present number of concentric actions in explosive exercise seems to have facilitated the neuromuscular system.

This study was conducted to assess the effects in trained cyclists of exhausting endurance cycle exercise (CE) on maximal isometric force production, surface electromyogram (EMG) and activation deficit (AD) of the knee extensors. Ten male subjects made four isometric maximal voluntary contractions (MVC) of the knee extensor muscles immediately prior (pre), 10 min after (post) and 6 h after completion of CE. The CE consisted of 30 min of exercise on a stationary cycle ergometer at an intensity corresponding to 80% of maximal oxygen uptake (VO(2max)) followed by four x 60-s periods at 120% of VO(2max). Two MVC were performed with recording of surface EMG from the knee extensors, whilst an additional two MVC were completed with percutaneous electrical muscle stimulation (EMS; 25 pulses at 100 Hz with the maximal tolerable current) superimposed over the maximal voluntary contraction force (MVF) but without EMG (to avoid interference). The MVF, integrated EMG (iEMG), and AD [calculated as the difference between MVF and the electrically stimulated force (ESF) during the EMS contractions] were statistically analysed. The MVF was significantly reduced (P < 0.05) post and 6-h post compared to pre-CE level. The iEMG was significantly reduced (P < 0.05) post and 6 h post CE. The ESF was also reduced, whilst AD was significantly increased (P < 0.05) post and 6-h post CE compared to the pre CE. These results suggest that the level of exercise stress administered in this study was sufficient to impair the central and peripheral mechanisms of force generation in knee extensors for a period of 6-h. Athletes engaged in concurrent training (strength and endurance) should consider this effect in exercise programming.

The purpose of this investigation was to determine the effect of involvement in power lifting, Olympic lifting, and sprinting on strength and power characteristics in the squat movement. A standard one repetition maximum squat test, jump squat tests, and vertical jumps with various loads were performed. The power lifters (PL, n 5 8), Olympic lifters (OL, n 5 6), and sprinters (S, n 5 6) were significantly stronger than the controls (C, n 5 8) (p # 0.05). In addition, the OL group was significantly stronger than the S group. The OL group produced significantly higher peak forces, power outputs, velocities, and jump heights in comparison to the PL and C groups for jump trials at various loads. The S group produced higher peak velocities and jump heights in comparison to the PL and C groups for jump trials at various loads. The PL group was significantly higher in peak force and peak power for jump trials at various loads in comparison to the C group. The data indicates that strength and power characteristics are specific to each group and are most likely influenced by the various training protocols utilized.

Exercise echocardiography was used to assess myocardial ischemia after non-Q-wave acute myocardial infarction in 40 consecutive patients. Resting parasternal long- and short-axis views and apical 4- and 2-chamber views were recorded, digitized, and stored. A maximal symptom-limited exercise test was performed within 21 days (mean 17.7 +/- 3) using a cycle ergometer with continuous monitoring and the echocardiogram was repeated in the same views. Resting and exercise echocardiograms were then compared. Coronary angiography was performed in all patients within 21 days of exercise echocardiography. Stenosis inor =50% of the lumen diameter was considered significant. Of the 40 patients studied, 29 (72%) had continuing angina and 11 (28%) had no angina. Eighteen patients (62%) with angina developed angina during exercise testing and 19 (65%) developed ST-segment depression. In patients without angina, 1 (9%) developed postexercise angina and 2 (18%) developed ST-segment depression. The mean wall motion score index after exercise increased from 1.2 +/- 0.3 to 1.8 +/- 0.4 in patients with continuing angina (p0.001) and from 1.2 +/- 0.3 to 1.4 +/- 0.3 in patients without angina (p = NS). Prolonged wall motion abnormalities lasting20 minutes persisted inor =1 segment in 27 of 29 patients (93%) with angina or in 2 of 1 1 patients (18%) without angina (p0.001). Patients with continued angina had predominantly 3-vessel coronary artery disease (22 of 29 [76%]) or 2-vessel disease (7 of 29 [24%]), and those without angina had 1-vessel disease (6 of 11 [55%]) or 2-vessel disease (4 of 11 [36%]). One patient had 3-vessel disease. The duration of wall motion abnormality demonstrated a significant relation to 2- and 3-vessel coronary artery disease (p0.001). Thus, patients with non-Q-wave acute myocardial infarction had a high incidence of multivessel coronary disease not necessarily detected on routine exercise testing. There was also a significant incidence of prolonged wall motion abnormality.

Background: It has been reported that intermittent exercise in hypoxia environments results inbetterweight loss effect thanexercise in normoxia conditions in individuals with obesity. There has also been a recent report that 1h of exercise in a hypoxic environment resulted in acute improvement of insulin sensitivity and short term glycaemia control in patients with Type 2 diabetes (T2DM). The aim of this study was to examine the effects of 4 weeks exposure to ahypoxic environment,withorwithout exercise, onglucose homeostasis and body weight in obese mice with T2DM. Methods: Eight-week old male C57BL/6 J mice were fed with a high-fat diet (14% protein, 21% carbohydrates and 55% fat) for approximately 12weeks to induce T2DM. Themicewere then allocated into three experimental groupswithn=7 in each: (1) hypoxia at 15%O2, no exercise, (2) hypoxia at 15%O2 and submaximal treadmill exercise and (3) normoxia and submaximal treadmill exercise. These groups were given the corresponding intervention with 1h per session, 6 sessions per week, for 4 weeks. There were also two control groups: (4) normal diet (14% protein, 72% carbohydrates and 4% fat) control group, and (5) diabetes control group (fed by the high-fat diet), both were not exposed to exercise and hypoxia. Before and after the 4 weeks intervention, mice were assessed for fasting blood glucose level (FBS) and weight change. Results: The 4 weeks intervention resulted in a significant reduction of FBS in the three intervention groups (from14.17, 13.74 and 13.87mmol/l to 9.43, 8.83 and 8.86mmol/l, respectively), but no significant difference between pre-FBS and post-FBS in control groups (9.1 vs 9.07mmol/l in the normal control group, and 13.56 vs 13.03mmol/l in the diabetes control group). The body weight of all groups increased during the intervention (an average of 20.8% of increase in the intervention groups, and 56.8% of increase in the control groups); however significant differences between preweight and post-weightwere only found in the two control groups. Discussion: The 4 weeks of intermittent hypoxia intervention reduced the FBS of the obese mice with T2DM to normal level, and sloweddown the trendof theirweight increase. The studyprovided new evidence that intermittent exposure to hypoxia environment for 4 weeks, with or without exercise, can be beneficial in management of blood glucose and body weight in mice with T2DM.

Background: Resistance training increases the skeletal muscle strength and functional ability of chronic heart failure patients. However, there is limited data regarding the effect of resistance training on the hemodynamic responses and peak oxygen consumption (peak VO2) of chronic heart failure patients treated with beta-blocker. This study examined the effect of resistance training on hemodynamics, peak aerobic capacity, muscle strength and quality of life of chronic heart failure patients on beta-blockers medication. Methods: Fifteen men diagnosed with chronic heart failure were matched to either a resistance training program or non-training control group. At baseline and after 8 weeks of resistance training patients performed both Balke incremental and maximal strength tests and completed quality of life questionnaires. Results: The resistance training group demonstrated a significant increase of walking time and peak VO2 by 11.7% (p=0.002) and ~19% (pb0.05), respectively Peak VO2 was significantly correlated with both walking time (r=0.54, p=0.038) and change in total weight lifted (r=0.55, p=0.034). Quality of life significantly increased by 87% (p=0.030). The improvement in quality of life was correlated with post training peak VO2 (r=0.58, p=0.025) and total weight lifted during the post maximal strength test (r=� 0.52, p=0.047). Conclusions: The benefits from resistance training for chronic heart failure patients on beta-blocker medication included an increased aerobic and exercise capacity, skeletal muscle strength and most importantly, an improvement in the quality of life, which is the main goal of cardiac rehabilitation programs. Furthermore, with appropriate supervision, it is recommended that resistance exercise be added to the exercise rehabilitation program of these patients when possible. D 2004 Elsevier Ireland Ltd. All rights reserved.

Introduction Adaptations to training include the accumulation of specific proteins. The ideal stimulus that alters gene transcriptive response that initiates changes in protein concentration is important for adaptations. Methods Fifty-four male Sprague-Dawley rats were randomly divided into 9 experimental groups, with six rats in each group. The groups consisted of: control (group 1); one day of training (2); continuous training groups who trained for a further two to four days followed by three days recovery (3,4,5,6); alternate-day training consisting of a further two to four sessions (7,8,9) finishing on the 7th day. On each training day, the animals ran on a motor driven treadmill at a slope of 10° and a speed of 19.3 m/min (∼76%VO2max) for 90 minutes. Gastrocnemius muscle specimens were obtained from the respective groups 1 hour after training ceased. Real-time PCR was performed to determine the changes (in fold) in mRNA of PGC1α, COXIV, CaMKIV, NRF1, AMPKα1 and α2, and mtTFA. One-way ANOVA was used in comparisons of the mean values between groups. Results The results showed that mRNA expression was significantly different between the training groups for AMPkα1 after the 3rd training session, PGC1α after the 4th training session, with CaMKIV significantly different following three days of recovery. COXIV and mtTFA were not significantly different at any point. Conclusions Training design has differing effects on expression of the selected genes. This data has provided insight into the importance of training design and recovery intervals which warrants a need for further research. Ethical Animal Research The study was approved by the Southern Cross University Animal Care and Ethics Committee. Sources of funding: None declared. Competing interests: none.

Existing literature suggests that tests for maximal oxygen uptake (VO2max) should last 8-12 minutes and that prolonged tests do not produce valid measurements. The research underlying this suggestion has been performed with non-athletic populations and trained athletes may be more tolerant of longer protocols. Eleven rowers (8 males, 3 females) each underwent four different incremental tests on a standard rowing ergometer in randomised counterbalanced order. One of the tests was continuous with workload increments each minute (IT1MIN). This test lasted an average of 12 min+/-4 s (SEM). The other three tests were discontinuous and consisted of 7 stages separated by 1-minute recovery intervals. Stage durations of 3, 4 and 5 min were used in the different tests (IT3MIN, IT4MIN and IT5MIN). Mean values for VO2max were almost identical for IT1MIN (4.56+/-0.22 L x min(-1)), IT3MIN (4.60+/-0.23 L x min(-1)) and IT4MIN (4.60+/-0.21 L x min(-1)), while IT5MIN produced a significantly lower value (4.47+/-0.21 L x min(-1), p

The purpose of this study was to investigate the recovery of muscle force generating capacity (FGC) of the lower limbs following a session of cycle exercise (CE). Fourteen male cyclists (mean +/- SD age 25 +/- 4 yrs and VO2max 65.8 +/- 5 ml x kg(-1)min(-1)) performed tests assessing lower limb muscle FGC at rest (pre-test), as well as 6 and 24 hrs following CE performed on a mechanically-braked cycle ergometer. The CE consisted of 30 min at a workload corresponding to the lactate (Dmax) threshold (+/-15 W), and four 60 s rides at 120% VO2max with one min rest between each ride. At the completion of the CE a 6 or 24 hr recovery period was initiated, after which, each subject's muscle FGC was measured. The analysis of lower limb muscle FGC included, (1) 6 s all-out cycle test; (2) a maximal isokinetic leg extension at 60, 120 and 180 degrees x s(-1); and (3) a maximal concentric squat jump. Statistical analysis showed that compared to pre-test levels, a significant reduction in both isokinetic peak torque at 60 degrees x s(-1) and isoinertial maximum force occurred after 6 hrs of recovery. Although not significant, reductions also occurred at 6 hrs of recovery in isokinetic peak torque at 120 and 180 degrees x s(-1), as well as maximum rate of force development (RFD) during the squat jumps. No significant differences were observed between isokinetic peak torque, maximum force or RFD pre-test and following the 24 hr recovery period, indicating these tests had returned to normal by this time. No significant differences were found between peak power (PP) during the 6 s cycle test, pre-test and following either 6 or 24 hrs of recovery. These findings confirm earlier research that maximal voluntary strength is reduced for at least 6 hours following exhaustive dynamic exercise. The reduction in muscle FGC should be considered when resistance training is scheduled after endurance exercise.

The purpose of this investigation was to examine the effect of an 8-week training program with heavy- vs. light-load jump squats on various physical performance measures and electromyography (EMG). Twenty-six athletic men with varying levels of resistance training experience performed sessions of jump squats with either 30% (JS30, n 5 9) or 80% (JS80, n 5 10) of their one repetition maximum in the squat (1RM) or served as a control (C, n 5 7). An agility test, 20m sprint, and jump squats with 30% (30J), 55% (55J), and 80% (80J) of their 1RM were performed before and after training. Peak force, peak velocity (PV), peak power (PP), jump height, and average EMG (concentric phase) were calculated for the jumps. There were significant increases in PP and PV in the 30J, 55J, and 80J for the JS30 group (p # 0.05). The JS30 group also significantly increased in the 1RM with a trend towards improved 20-m sprint times. In contrast, the JS80 group significantly increased both PF and PP in the 55J and 80J and significantly increased in the 1RM but ran significantly slower in the 20-m sprint. In the 30J the JS30 group’s percentage increase in EMG activity was significantly different from the C group. In the 80J the JS80 group’s percentage increase in EMG activity was significantly different from the C group. This investigation indicates that training with light-load jump squats results in increased movement velocity capabilities and that velocity-specific changes in muscle activity may play a key role in this adaptation.