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17 results on '"Aljaddawi, Abdullah A."'

4. Simple Protocol for immunoglobulin G Purification from Camel 'Camelus dromedarius' Serum

Author

Shawki Asmaa, Abd El-Baky Nawal, Ahmed Mohammed, Linjawi Mustafa H., Aljaddawi Abdullah A., and Redwan Elrashdy M.

Subjects
camelus dromedarius, camel igg purification, caprylic acid, temperature, stirring, Biology (General), QH301-705.5
Abstract

The present study aimed to describe and standardize a simple and efficient protocol for purification of camel IgG from serum, which can be applied for Camilidae antibody production in research laboratories, the preindustrial stage. Camel serum IgG was separated with caprylic acid and ammonium sulfate, then the effect of four variables studied: caprylic acid concentration, pH, stirring time, and stirring intensity. Camel IgG prepared by standardized caprylic acid fractionation method for camel serum was compared with commercial anti-sera products. Camel IgG purification from undiluted sera using caprylic acid at concentration of 8% v/v gave the best results. Purification at different pH values using caprylic acid at 8% v/v revealed that pH 5.5 was optimal. Investigating purification at different stirring time intervals using 8% v/v caprylic acid at pH 5.5 demonstrated that stirring for 90 min gave the optimum results. Finally, studying purification at different stirring intensities using 8% v/v caprylic acid at pH 5.5 for 90 min, the best stirring intensity was at 450 rpm. Overall, the results suggest that caprylic acid purification of camel serum IgG is more effective and safe than ammonium sulfate method in simplicity, purity, and lower non-IgG proteins in the final preparation with lower protein aggregates.

Published
2017
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8. Assaying for antiviral activity of the folkloric medicinal desert plant Rhazya stricta on coronavirus SARS-CoV-2

Author

Baeshen, Mohammed N., primary, Attar, Roba, additional, Bouback, Thamer A., additional, Albeshri, Abdulaziz O., additional, Baeshen, Naseebh N., additional, Karkashan, Alaa, additional, Abbas, Basma, additional, Aljaddawi, Abdullah A., additional, Almulaiky, Yaaser Q., additional, Mahmoud, Sara H., additional, Abo Shama, Noura M., additional, Ali, Mohamed A., additional, Baadhaim, Moayad, additional, Zakri, Samer, additional, Alsayegh, Khaled, additional, Mohammed, Arif, additional, and Baeshen, Nabih A., additional

Published
2022
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10. In silico Screening of Some Compounds Derived from the Desert Medicinal Plant Rhazya stricta for Potential Treatment of COVID -19

Author

Baeshen, Nabih A., primary, Albeshri, Abdulaziz O., additional, Baeshen, Naseebh N., additional, Attar, Roba, additional, Karkashan, Alaa, additional, Abbas, Basma, additional, Bouback, Thamer A., additional, Aljaddawi, Abdullah A., additional, Refai, Mohammed Y., additional, Abdelkader, Hayam S., additional, Tamim, Abdullah Al, additional, Alowaifeer, Abdullah, additional, and Baeshen, Mohammed N., additional

Published
2021
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11. Assaying for antiviral activity of the folkloric medicinal desert plant Rhazya strictaon coronavirus SARS-CoV-2

Author

Baeshen, Mohammed N., Attar, Roba, Bouback, Thamer A., Albeshri, Abdulaziz O., Baeshen, Naseebh N., Karkashan, Alaa, Abbas, Basma, Aljaddawi, Abdullah A., Almulaiky, Yaaser Q., Mahmoud, Sara H., Abo Shama, Noura M., Ali, Mohamed A., Baadhaim, Moayad, Zakri, Samer, Alsayegh, Khaled, Mohammed, Arif, and Baeshen, Nabih A.

Abstract

AbstractThe emergence of superbugs and resistant pathogens poses a challenge in scientific and medical research as they threaten public health worldwide. Many herbal natural products currently used in therapies have been suggested to exert antimicrobial, antiviral and even virucidal activities against a vast majority of impervious pathogens. Rhazya stricta, a folk medicinal desert plant from Saudi Arabia was recently revealed to exhibit bactericidal activity against multidrug-resistant (MDR) microorganisms. The pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a threat to public health worldwide. Hence, we examined the antiviral activity of R. strictaagainst the virus. The R. strictawater extract was prepared at the traditional dose. The antagonistic effects of this extract against pathogens have been proven in previous studies, and those against SARS-CoV-2 were shown in the present study. Therefore, we explored the effects of the plant extracts and fractions against the virus for future drug development. All plant extracts showed antiviral effects against SARS-CoV-2 in the Vero E6 cell lines. Non-alkaloids showed the strongest effect against the virus, followed by weak base alkaloids and finally strong base alkaloids. A cytotoxicity assay was performed to explore the safest dose with the strongest antiviral effects. The non-alkaloid extract derived from R. strictaleaves is a promising antiviral candidate for the development of potential drugs with appropriate activity against COVID-19 and other life-threatening diseases.

Published
2022
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