11 results on '"Alison E. Seline"'
Search Results
2. Depressed shiny scars and crusted erosions
- Author
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Olayemi Sokumbi, Alison E. Seline, and Christina Dai
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,Protoporphyria, Erythropoietic ,business.industry ,Biopsy ,Scars ,Dermatology ,Cicatrix ,medicine ,Humans ,Female ,medicine.symptom ,business ,Child - Published
- 2020
3. Langerhans cell histiocytosis with prominent nail involvement
- Author
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Dawn H. Siegel, Olayemi Sokumbi, Alison E. Seline, and Nicole R. Bender
- Subjects
Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Histology ,business.industry ,Dermatology ,medicine.disease ,Pathology and Forensic Medicine ,Histiocytosis, Langerhans-Cell ,Nail Diseases ,030207 dermatology & venereal diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Langerhans cell histiocytosis ,medicine ,Nail (anatomy) ,Humans ,Child ,business - Published
- 2018
4. Cutaneous metastasis of breast adenoid cystic carcinoma to the scalp
- Author
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Anthony J. Little, Brian L. Swick, Karolyn A. Wanat, and Alison E. Seline
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Histology ,business.industry ,Adenoid cystic carcinoma ,Dermatology ,Disease ,medicine.disease ,behavioral disciplines and activities ,Pathology and Forensic Medicine ,Metastasis ,stomatognathic diseases ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,nervous system ,030220 oncology & carcinogenesis ,Scalp ,Medicine ,business ,Cutaneous metastasis ,human activities ,psychological phenomena and processes - Abstract
Adenoid cystic carcinoma (ACC) is a tumor that can be of primary cutaneous origin or secondary to metastatic disease, most commonly salivary origin. Aside from primary cutaneous and salivary types, ACC of the breast is a rare, more indolent variant. Cutaneous metastases secondary to breast ACC is exceedingly uncommon and not previously reported to our knowledge. We present the case of a 67-year-old woman who developed cutaneous metastasis from primary breast ACC.
- Published
- 2016
5. Photopolymerized microfeatures guide adult spiral ganglion and dorsal root ganglion neurite growth
- Author
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Mark Ramirez, C. Allan Guymon, Braden Leigh, Marlan R. Hansen, Linjing Xu, and Alison E. Seline
- Subjects
0301 basic medicine ,Neurite ,Polymers ,Article ,03 medical and health sciences ,0302 clinical medicine ,Dorsal root ganglion ,Ganglia, Spinal ,otorhinolaryngologic diseases ,Electrode array ,Neurites ,Medicine ,Animals ,Inner ear ,Spiral ganglion ,Cells, Cultured ,business.industry ,Guided Tissue Regeneration ,Sensory Systems ,Nerve Regeneration ,030104 developmental biology ,Neurite growth ,medicine.anatomical_structure ,Cochlear Implants ,Otorhinolaryngology ,Auditory stimuli ,sense organs ,Neurology (clinical) ,Hearing perception ,business ,Spiral Ganglion ,Neuroscience ,030217 neurology & neurosurgery - Abstract
HYPOTHESIS Microtopographical patterns generated by photopolymerization of methacrylate polymer systems will direct growth of neurites from adult neurons, including spiral ganglion neurons (SGNs). BACKGROUND Cochlear implants (CIs) provide hearing perception to patients with severe to profound hearing loss. However, their ability to encode complex auditory stimuli is limited due, in part, to poor spatial resolution caused by spread of the electrical currents in the inner ear. Directing the regrowth of SGN peripheral processes towards stimulating electrodes could help reduce current spread and improve spatial resolution provided by the CI. Previous work has demonstrated that micro- and nano-scale patterned surfaces precisely guide the growth of neurites from a variety of neonatal neurons including SGNs. Here, we sought to determine the extent to which adult neurons likewise respond to these topographical surface features. METHODS Photopolymerization was used to fabricate methacrylate polymer substrates with micropatterned surfaces of varying amplitudes and periodicities. Dissociated adult dorsal root ganglion neurons (DRGNs) and SGNs were cultured on these surfaces and the alignment of the neurite processes to the micropatterns was determined. RESULTS Neurites from both adult DRGNs and SGNs significantly aligned to the patterned surfaces similar to their neonatal counterparts. Further DRGN and SGN neurite alignment increased as the amplitude of the microfeatures increased. Decreased pattern periodicity also improved neurite alignment. CONCLUSION Microscale surface topographic features direct the growth of adult SGN neurites. Topographical features could prove useful for guiding growth of SGN peripheral axons towards a CI electrode array.
- Published
- 2018
6. Neural Pathfinding on Uni- and Multidirectional Photopolymerized Micropatterns
- Author
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Andrew M. Erwood, Marlan R. Hansen, Scott P. White, Alison E. Seline, Linjing Xu, C. Allan Guymon, and Bradley W. Tuft
- Subjects
Materials science ,Neurite ,Neural Prostheses ,Retinal implant ,Nanotechnology ,Biocompatible Materials ,02 engineering and technology ,pattern ,Signal ,03 medical and health sciences ,otorhinolaryngologic diseases ,medicine ,Neurites ,Animals ,General Materials Science ,Inner ear ,Spiral ganglion ,Cells, Cultured ,030304 developmental biology ,0303 health sciences ,neurite ,Neural Prosthesis ,021001 nanoscience & nanotechnology ,Nerve Regeneration ,Rats ,contact guidance ,medicine.anatomical_structure ,photopolymerization ,microsurface topography ,Axon guidance ,sense organs ,Schwann Cells ,0210 nano-technology ,Pathfinding ,Spiral Ganglion ,neural prosthesis ,Biomedical engineering ,Research Article - Abstract
Overcoming signal resolution barriers of neural prostheses, such as the commercially available cochlear impant (CI) or the developing retinal implant, will likely require spatial control of regenerative neural elements. To rationally design materials that direct nerve growth, it is first necessary to determine pathfinding behavior of de novo neurite growth from prosthesis-relevant cells such as spiral ganglion neurons (SGNs) in the inner ear. Accordingly, in this work, repeating 90° turns were fabricated as multidirectional micropatterns to determine SGN neurite turning capability and pathfinding. Unidirectional micropatterns and unpatterned substrates are used as comparisons. Spiral ganglion Schwann cell alignment (SGSC) is also examined on each surface type. Micropatterns are fabricated using the spatial reaction control inherent to photopolymerization with photomasks that have either parallel line spacing gratings for unidirectional patterns or repeating 90° angle steps for multidirectional patterns. Feature depth is controlled by modulating UV exposure time by shuttering the light source at given time increments. Substrate topography is characterized by white light interferometry and scanning electron microscopy (SEM). Both pattern types exhibit features that are 25 μm in width and 7.4 ± 0.7 μm in depth. SGN neurites orient randomly on unpatterned photopolymer controls, align and consistently track unidirectional patterns, and are substantially influenced by, but do not consistently track, multidirectional turning cues. Neurite lengths are 20% shorter on multidirectional substrates compared to unidirectional patterns while neurite branching and microfeature crossing events are significantly higher. For both pattern types, the majority of the neurite length is located in depressed surface features. Developing methods to understand neural pathfinding and to guide de novo neurite growth to specific stimulatory elements will enable design of innovative biomaterials that improve functional outcomes of devices that interface with the nervous system.
- Published
- 2014
7. Nf2 Mutation in Schwann Cells Delays Functional Neural Recovery Following Injury
- Author
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Brian Mostaert, J. Jason Clark, Douglas J. Van Daele, Kristy Truong, Iram Ahmad, Tyler Bertroche, Marlan R. Hansen, and Alison E. Seline
- Subjects
0301 basic medicine ,Genetically modified mouse ,Time Factors ,Neural Conduction ,Mice, Transgenic ,Biology ,Motor Activity ,Article ,03 medical and health sciences ,Myelin ,0302 clinical medicine ,medicine ,otorhinolaryngologic diseases ,Animals ,Protein Isoforms ,Neurofibromatosis type 2 ,Axon ,Neurofibromin 2 ,General Neuroscience ,Regeneration (biology) ,Recovery of Function ,Nerve injury ,medicine.disease ,Sciatic Nerve ,Axons ,Cell biology ,Nerve Regeneration ,Merlin (protein) ,030104 developmental biology ,medicine.anatomical_structure ,Mutation ,Sciatic nerve ,Schwann Cells ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
Merlin is the protein product of the NF2 tumor suppressor gene. Germline NF2 mutation leads to neurofibromatosis type 2 (NF2), characterized by multiple intracranial and spinal schwannomas. Patients with NF2 also frequently develop peripheral neuropathies. While the role of merlin in SC neoplasia is well established, its role in SC homeostasis is less defined. Here we explore the role of merlin in SC responses to nerve injury and their ability to support axon regeneration. We performed sciatic nerve crush in wild-type (WT) and in P0SchΔ39–121 transgenic mice that express a dominant negative Nf2 isoform in SCs. Recovery of nerve function was assessed by measuring mean contact paw area on a pressure pad 7, 21, 60, and 90 days following nerve injury and by nerve conduction assays at 90 days following injury. After 90 days, the nerves were harvested and axon regeneration was quantified stereologically. Myelin ultrastructure was analyzed by electron microscopy. Functional studies showed delayed nerve regeneration in Nf2 mutant mice compared to the WT mice. Delayed neural recovery correlated with a reduced density of regenerated axons and increased endoneurial space in mutants compared to WT mice. Nevertheless, functional and nerve conduction measures ultimately recovered to similar levels in WT and Nf2 mutant mice, while there was a small (∼17%) reduction in the percent of regenerated axons in the Nf2 mutant mice. The data suggest that merlin function in SCs regulates neural ultrastructure and facilitates neural regeneration, in addition to its role in SC neoplasia.
- Published
- 2016
8. Postprocedural Blue Toes
- Author
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Grant K. Ghahramani, Karolyn A. Wanat, and Alison E. Seline
- Subjects
Male ,medicine.medical_specialty ,030232 urology & nephrology ,Myocardial Infarction ,030204 cardiovascular system & hematology ,Coronary Angiography ,Coronary artery disease ,03 medical and health sciences ,Coronary artery bypass surgery ,0302 clinical medicine ,Medicine ,Humans ,Myocardial infarction ,Coronary Artery Bypass ,Blood urea nitrogen ,Aged ,Embolism, Cholesterol ,business.industry ,Acute kidney injury ,General Medicine ,Toes ,medicine.disease ,Hand ,Surgery ,Stenosis ,medicine.anatomical_structure ,business ,Cholesterol embolism ,Pigmentation Disorders ,Artery - Abstract
A man in his 70s with a history of coronary artery disease, hypertension, and mild aortic stenosis presented with altered mental status, acute kidney injury requiring dialysis, and painful, dusky skin discoloration on his distal extremities. Three weeks prior, he had undergone coronary angiography and coronary artery bypass graft surgery after myocardial infarction. On physical examination, he had exquisitely tender blue macules, patches, and papules on the plantar aspect of several toes (Figure 1A) and red, tender macules on his hands (Figure 1B). Laboratory examination was notable for a white blood cell count of 9100/μL with 22% eosinophils, blood urea nitrogen level of 70 mg/dL (25 mmol/L), and creatinine level of 6.1 mg/dL (539.2 μmol/L). Urinalysis was remarkable for 1+ blood and 2+ protein.
- Published
- 2016
9. Secondary syphilis
- Author
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Alison E, Seline and Brian L, Swick
- Subjects
Humans ,Dermatitis ,Syphilis - Published
- 2016
10. Surface features of the lipid droplet mediate perilipin 2 localization
- Author
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Alison E. Seline, Arthur C. Sletten, Andrew K. Rudd, Laura Listenberger, and Michelle M. Logsdon
- Subjects
endocrine system ,Perilipin 2 ,Static Electricity ,Biophysics ,Phospholipid ,Gene Expression ,Plasma protein binding ,Biology ,Biochemistry ,complex mixtures ,Perilipin-2 ,Article ,chemistry.chemical_compound ,Lipid droplet ,Humans ,Molecular Biology ,Liposome ,Binding Sites ,Vesicle ,technology, industry, and agriculture ,Membrane Proteins ,Cell Biology ,Lipid Droplets ,eye diseases ,Cell biology ,HEK293 Cells ,chemistry ,Membrane protein ,Liposomes ,Proteolysis ,biology.protein ,Perilipin ,Phosphatidylcholines ,lipids (amino acids, peptides, and proteins) ,Hydrophobic and Hydrophilic Interactions ,Protein Binding - Abstract
All eukaryotic organisms store excess lipid in intracellular lipid droplets. These dynamic structures are associated with and regulated by numerous proteins. Perilipin 2, an abundant protein on most lipid droplets, promotes neutral lipid accumulation in lipid droplets. However, the mechanism by which perilipin 2 binds to and remains anchored on the lipid droplet surface is unknown. Here we identify features of the lipid droplet surface that influence perilipin 2 localization. We show that perilipin 2 binding to the lipid droplet surface requires both hydrophobic and electrostatic interactions. Reagents that disrupt these interactions also decrease binding. Moreover, perilipin 2 binding does not depend on other lipid droplet-associated proteins but is influenced by the lipid composition of the surface. Perilipin 2 binds to synthetic vesicles composed of dioleoylphosphatidylcholine, a phospholipid with unsaturated acyl chains. Decreasing the temperature of the binding reaction, or introducing phospholipids with saturated acyl chains, decreases binding. We therefore demonstrate a role for surface lipids and acyl chain packing in perilipin 2 binding to lipid droplets. The ability of the lipid droplet phospholipid composition to impact protein binding may link changes in nutrient availability to lipid droplet homeostasis.
- Published
- 2014
11. Hydrophobic and electrostatic interactions anchor perilipin 2 to the surface of cytosolic lipid droplets
- Author
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Arthur C. Sletten, Laura L. Listenberger, and Alison E. Seline
- Subjects
Cytosol ,biology ,Biochemistry ,Chemistry ,Lipid droplet ,Perilipin 2 ,Genetics ,Biophysics ,biology.protein ,Electrostatics ,Molecular Biology ,Biotechnology - Published
- 2012
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