1. The Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile.
- Author
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Sebastian Thieme, Alexander Holzbaur, Ralf Wiedemuth, Aline Binner, Katrin Navratiel, Konstantinos Anastassiadis, Sebastian Brenner, and Cornelia Richter
- Subjects
Medicine ,Science - Abstract
Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo. We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
- Published
- 2018
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