1. Effectiveness of strict anti-contamination criteria for non-invasive foetal sex determination in a clinical setting
- Author
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Adolfo Allegra, Alicia González-Liñán, Lucio Trizzino, Daniela Giambelluca, and Angelo Marino
- Subjects
Male ,Sex Determination Analysis ,medicine.medical_specialty ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Fetus ,Pregnancy ,Prenatal Diagnosis ,Humans ,Medicine ,False Positive Reactions ,Good practice ,Gynecology ,business.industry ,Obstetrics ,Non invasive ,Reproducibility of Results ,Obstetrics and Gynecology ,Gestational age ,DNA Contamination ,Sex-Determining Region Y Protein ,Pregnancy Trimester, First ,Cell-free fetal DNA ,Practice Guidelines as Topic ,Pediatrics, Perinatology and Child Health ,Female ,business ,Blood Chemical Analysis - Abstract
Objective: The aim of this study is to prevent false positive results in non-invasive foetal sex determination from pregnant women’s plasma following strict anti-contamination criteria.Methods: cffDNA from 200 pregnant women (mean gestational age of 8.3 ± 0.2 weeks) was analyzed based on genetic forensic anti-contamination procedures and classical non-invasive foetal sex determination techniques.Results: No false positive or false negative was reported. The sensitivity and the specificity reached both a 100% value.Conclusions: Prevention of contamination following our anti-contamination criteria is a good practice for certain non-invasive sex determination, using cffDNA.
- Published
- 2013
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