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Agrosilvopasture is one part of the agroforestry system that can be offered to overcome the problems of food availability, land ownership, and decreasing soil fertility. The agrosilvopasture system itself can be applied to various types of land, including dry land such as in Paradowane Village, Parado District, Bima Regency. In the village there is a forest farmer group that manages forest areas using the agrosilvopastura system, namely KTH Sorowua. This study analyzes the income and welfare of farmers by comparing the income of farmers before and after implementing the agrosilvopastura system in KTH Sorowua, Paradowane Village, Parado District, Bima Regency. The research data is then analyzed SWOT to get the right strategy to improve the welfare and income of farmers. The results of the SWOT analysis provide an overview of the application of the agrosilvopasture system in general, where the agrosilvopasture system can increase the income and welfare of KTH Sorowua, Paradowane Village, Parado District, Bima Regency, even though the agrosilvopasture system has not been implemented properly.

A polyphasic study was designed to determine the taxonomic provenance of a strain, isolate PRKS01-29T, recovered from an Indonesian sand dune and provisionally assigned to the Streptomyces violaceusniger clade. Genomic, genotypic and phenotypic data confirmed this classification. The isolate formed an extensively branched substrate mycelium which carried aerial hyphae that differentiated into spiral chains of rugose ornamented spores, contained LL-as the wall diaminopimelic acid, MK-9 (H6, H8) as predominant isoprenologues, phosphatidylethanolamine as the diagnostic phospholipid and major proportions of saturated, iso- and anteiso- fatty acids. Whole-genome sequences generated for the isolate and Streptomyces albiflaviniger DSM 41598T and Streptomyces javensis DSM 41764T were compared with phylogenetically closely related strains, the isolate formed a branch within the S. violaceusniger clade in the resultant phylogenomic tree. Whole-genome sequences data showed that isolate PRKS01-29T was most closely related to the S. albiflaviniger strain but was distinguished from the latter and from other members of the clade using combinations of phenotypic properties and average nucleotide identity and digital DNA:DNA hybridization scores. Consequently, it is proposed that isolate PRKS01-29T (= CCMM B1303T = ICEBB-02T = NCIMB 15210T) should be classified in the genus Streptomyces as Streptomyces sabulosicollis sp. nov. It is also clear that streptomycetes which produce spiral chains of rugose ornamented spores form a well-defined monophyletic clade in the Streptomyces phylogenomic tree., the taxonomic status of which requires further study. The genome of the type strain of S. sabulosicollis contains biosynthetic gene clusters predicted to produce new natural products.

Streptomyces is a filamentous bacteria genus belonging to the Streptomycetaceae family and there are a lot of different species Streptomyces of that have been discovered. Streptomyces sp. SUK 48 is an endophytic bacteria which found in a fruit of Brasilia sp. that located at UKM reserved forest known as Bangi Reserved Forest [1]. This study was conducted to determine the classification of Streptomyces sp. SUK 48 isolated from fruit of Brasilia sp. tree, in genome perspectives [2]. In specific, the objectives of this study were to construct phylogenetic tree for Streptomyces SUK 48, compare genome Streptomyces SUK 48 with other related Streptomyces species genome using bioinformatic tools that can do phylogenomic research and determine Average Nucleotide Identity (ANI) score of Streptomyces sp. SUK 48 as compared to others Streptomyces spp. Based on phylogenetic analysis, Streptomyces SUK 48 have high similarities with Streptomyces malaysiense MUSC 136 with 99.65% pairwise similarity which obtained from BLASTN analysis. While, phylogenomic analysis showed Streptomyces SUK 48 has high similarity with Streptomyces murinus that observed in phylogenomic tree GToTree (Figure 1). Based on the ANI score, it shows that Streptomyces SUK 48 have similarities of 94.14% with Streptomyces murinus from the phylogenomics analysis (Table 1). In conclusion, Streptomyces SUK 48 is a potential novel isolated species.

A polyphasic study was undertaken to establish the position of a Streptomyces strain, isolate PRKS01-65T, recovered from sand dune soil collected at Parangkusumo, Yogyakarta Province, Java, Indonesia. A combination of chemotaxonomic, cultural and morphological properties confirmed its position in the genus of Streptomyces . Comparative 16S rRNA gene sequence analyses showed that the isolate was most closely related to Streptomyces leeuwenhoekii C34T (99.9 % similarity) and loosely associated with the type strains of Streptomyces chiangmaiensis (98.7 % similarity) and Streptomyces glomeratus (98.9 % similarity). Multilocus sequence analyses based on five conserved housekeeping gene alleles confirmed the close relationship between the isolate and S. leeuwenhoekii C34T, although both strains belonged to a well-supported clade that encompassed the type strains of S. glomeratus , Streptomyces griseomycini , Streptomyces griseostramineus , Streptomyces labedae , Streptomyces lomondensis and Streptomyces spinoverrucosus . A comparison of the draft genome sequence generated for the isolate with corresponding whole genome sequences of its closest phylogenomic neighbours showed that it formed a well-separated lineage with S. leeuwenhoekii C34T. These strains can also be distinguished using a combination of phenotypic properties and by average nucleotide identity and digital DNA–DNA hybridization similarities of 94.3 and 56 %, values consistent with their classification in different species. Based on this wealth of data it is proposed that isolate PRKS01-65T (=NCIMB 15211T=CCMM B1302T=ICEBB-03T) be classified as Streptomyces harenosi sp. nov. The genome of the isolate contains several biosynthetic gene clusters with the potential to produce new natural products.

Udang merupakan salah satu komoditas unggulan dalam sektor perikanan Negara Indonesia. Produksi hasil budidaya udang diharapkan mampu untuk memenuhi kebutuhan dalam negeri. Akan tetapi yang banyak terjadi adalah masih tingginya jumlah kematian udang akibat penyakit vibriosis dimana sebagian besar disebabkan oleh serangan bakteri Vibrio sp. Penelitian ini dilakukan bertujuan untuk mengetahui potensi kombinasi antara ekstrak daun kirinyuh sebagai antibakteridan ekstrak daun sirih yang mengandung senyawa antiseptik sebagai formula obat untuk penyakit vibriosis. Metode yang digunakan dalam penelitian ini adalah dengan mengekstraksi daun kirinyuhdan sirihdengan menggunakan ekstraktor soxhlet kemudian diuji secara in vitro, dan in vivo dengan terlebih dahulu melakukan uji patogenesitas pada tubuh udang dengan metode perendaman kemudian udang dipelihara dalam sebuah akuarium mini seperti simulasi tambak yang telah diberi kombinasi ekstrak tersebut dengan beberapa perlakuan. Hasil uji in vitro, perbandingan terbaik yang menghasilkan zona hambat terluas yaitu 1:1 sebesar 23 mm sehingga termasuk dalam golongan zat aktif sangat kuat dalam menghambat aktivitas bakteri. Sedangkan dari hasil uji in vivo perbandingan terbaik yang menghasilkan jumlah udang yang bertahan hidup terbanyak dan kematian udang terkecil yaitu 1:1.Pemberian ekstrak daun kirinyuh dan sirih memberikan pengaruh dalam menghambat pertumbuhan Vibrio sp. pada kedua pengujian.

A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.

Luwak coffee is a highly-priced coffee produced exclusively by the palm civet or luwak ( Paradoxurus hermaphrodites ssp.). The purpose of this study was to determine the diversity of culturable bacteria in the gastro intestinal tract of luwak. The bacterial isolates were phenotypically characterized by their morphology and molecularly by analysis of their1,500bp 16s rDNA sequence. The results showed that Enterobacter cloacae and Lactobacillus brevis were found all over luwak’s digestive tract . Enterobacter cloacae was the most common species. The most diverse bacterial population was found in small intestine. Seven bacterial generawere successfully identified from the small intestine and colon, compared to only five genera found in the stomach.