22 results on '"Ali, Ibne K."'
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2. List of Contributors
- Author
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Acosta, Anna M., primary, Acuin, Jose M., additional, Adam, Rodney D., additional, Afroze, Farzana, additional, Ahmed, Nadia, additional, Ahmed, Sabeena, additional, Ahmed, Tahmeed, additional, Ahmed, A.M. Shamsir, additional, Ali, S. Asad, additional, Ali, Ibne K., additional, Alroy, Karen A., additional, Ananthakrishnan, Ashwin N., additional, Ansong, Daniel, additional, Anstead, Gregory M., additional, Appleby, Laura J., additional, Armah, George E., additional, Aronson, Naomi E., additional, Aston, Stephen J., additional, Barnett, Elizabeth D., additional, Bartelt, Luther A., additional, Bates, Imelda, additional, Bausch, Daniel G., additional, Beadling, Charles W., additional, Beeching, Nicholas J., additional, Bennish, Michael L., additional, Bern, Caryn, additional, Bernstein, Wendy B., additional, Bird, Brian H., additional, Bloom, Allyson K., additional, Bodeker, Gerard, additional, Boyer Chammard, Timothée, additional, Bradsher, Robert W., additional, Brooker, Simon J., additional, Brooks, W. Abdullah, additional, Brouqui, Philippe, additional, Brown, Michael, additional, Brown, Michael R., additional, Broyles, Laura N., additional, Bruschi, Fabrizio, additional, Bundy, Donald A.P., additional, Burton, Matthew, additional, Cabrera-Sosa, Luis, additional, Callahan, Michael V., additional, Carapetis, Jonathan R., additional, Cardemil, Cristina V., additional, Carrol, Enitan D., additional, Caswell, Rachel, additional, Caumes, Eric, additional, Cavalheiro, Ana P., additional, Chan, Abner L., additional, Charunwatthana, Prakaykaew, additional, Checkley, Anna M., additional, Chen, Lin H., additional, Chher, Tepirou, additional, Chiong, Charlotte M., additional, Chisti, M. Jobayer, additional, Christiani, David C., additional, Clark, Taryn N., additional, Connor, Bradley A., additional, Conway, Devin J., additional, Cooper, Philip J., additional, Cope, Jennifer R., additional, Coughlin, R. Richard, additional, Coulibaly, Yaya I., additional, Coyle, Christina M., additional, Crozier, Ian, additional, Cunliffe, Nigel A., additional, Cupido, Blanche, additional, Curren, Emily J., additional, Danta, Mark, additional, Day, Nicholas P.J., additional, Debboun, Mustapha, additional, DeFraites, Robert F., additional, Dekumyoy, Paron, additional, del Castillo, Maria, additional, den Hoed, Caroline M., additional, de Silva, Nilanthi, additional, Deye, Gregory, additional, Dillingham, Rebecca A., additional, Drancourt, Michel, additional, Durward, Callum S., additional, Eddleston, Michael, additional, El-Kamary, Samer S., additional, Elshaboury, Ramy H., additional, Endtz, Hubert P., additional, Endy, Timothy P., additional, Fang, Shona C., additional, Fawzi, Wafaie, additional, Feasey, Nicholas A., additional, Field, Vanessa K., additional, Fischer, Marc, additional, Forsyth, Kevin, additional, Fournier, Pierre-Edouard, additional, Friedlander, Arthur M., additional, Furin, Jennifer J., additional, Gandhi, Ronak G., additional, Garcia, Hector H., additional, Garcia, Lynne S., additional, Geretti, Anna Maria, additional, Gikas, Achilleas, additional, Gilman, Robert H., additional, Giri, Sidhartha, additional, Gkika, Meropi, additional, Gordon, Melita A., additional, Gosselin, Richard A., additional, Gotuzzo, Eduardo, additional, Gould, Carolyn V., additional, Graeff-Teixeira, Carlos, additional, Graham, Stephen M., additional, Grant, Alison D., additional, Graybill, John R., additional, Graz, Bertrand, additional, Green, Stephen T., additional, Griffiths, Jeffrey K., additional, Griffiths, Michael J., additional, Gryseels, Bruno, additional, Gubler, Duane J., additional, Guhadasan, Rathi, additional, Hall, Aron J., additional, Hamer, Davidson H., additional, Hand, Robert M., additional, Harley, David, additional, Harris, Jason B., additional, Hassall, Oliver, additional, Hay, Roderick J., additional, Hickey, Patrick, additional, Hill, David R., additional, Hills, Susan L., additional, Hobdell, Martin H., additional, Hochberg, Natasha S., additional, Hopkins, Donald R., additional, Hossain, M. Iqbal, additional, Hotez, Peter J., additional, Howard, Cynthia R., additional, Hu, Victor, additional, Hung, Chien-Ching, additional, Islam, Munirul, additional, Iturriza-Gómara, Miren, additional, Joekes, Elizabeth, additional, Johnston, Victoria, additional, Jose, Jo-Ann, additional, Junghanss, Thomas, additional, Kamgno, Joseph, additional, Kampondeni, Sam, additional, Kang, Gagandeep, additional, Kazanjian, Powel, additional, Keshtkar-Jahromi, Maryam, additional, Keshtkar-Jahromi, Marzieh, additional, Keystone, Jay S., additional, Kim, Arthur Y., additional, Kim, Sung-Han, additional, King, Christopher L., additional, Kittitrakul, Chatporn, additional, Kleine, Christian, additional, Klion, Amy D., additional, Knight, Richard, additional, Koren, Michael, additional, Kottilil, Shyamasundaran, additional, Krause, Peter J., additional, Krishna, Sanjeev, additional, Kuhn, Jens H., additional, Kuipers, Ernst J., additional, LaBeaud, Angelle D., additional, Labra, Patrick John P., additional, Lalloo, David G., additional, Lambert, Saba, additional, Lanternier, Fanny, additional, LaRocque, Regina C., additional, Last, Anna, additional, Lawrenson, John, additional, Le, Thuy, additional, Lee, Keun Hwa, additional, Lewis, David A., additional, Libraty, Daniel H., additional, Lo, Nathan C., additional, Lockwood, Diana N.J., additional, Lockwood, Stephen J., additional, Lommerse, Kinke, additional, López-Vélez, Rogelio, additional, Lortholary, Olivier, additional, Mabey, David, additional, Magill, Alan J., additional, Maguiña, Ciro P., additional, Manji, Hadi, additional, Marks, Michael, additional, Maurin, Max, additional, Mayaud, Philippe, additional, Mayosi, Bongani M., additional, M'baya, Bridon, additional, McCarthy, Matthew W., additional, McCartney, Daniel, additional, McCormick, Joseph B., additional, McKew, Stephen, additional, McLellan, Susan L.F., additional, McMinn, Peter C., additional, Mertz, Gregory, additional, Milner, Danny A., additional, Molyneux, Elizabeth M., additional, Montgomery, Susan P., additional, Moonah, Shannon, additional, Moss, William J., additional, Murrell, K. Darwin, additional, Nanda, Neha, additional, Navarro, Eileen E., additional, Ndayizeye, Leonard, additional, Neafie, Ronald C., additional, Negroni, Ricardo, additional, Nelson, Ann M., additional, Newton, Paul N., additional, Nichol, Stuart T., additional, Norman, Francesca F., additional, Nunes, Marcio R.T., additional, Nutman, Thomas B., additional, Nyirenda, Tonney S., additional, Ochoa, Theresa J., additional, O'Farrell, Nigel, additional, Olayemi, Edeghonghon, additional, Oldfield, Edward C., additional, Omidian, Zahra, additional, Ordaya, Eloy E., additional, Paddock, Christopher D., additional, Paessler, Slobodan, additional, Papanikolaou, Ilias C., additional, Paris, Luc, additional, Parry, Christopher M., additional, Patel, Pragna, additional, Peacock, Sharon J., additional, Peeling, Rosanna W., additional, Persson, Hans, additional, Phillips, Jonathan J., additional, Phillips, Richard O., additional, Poovorawan, Kittiyod, additional, Powers, Ann M., additional, Qamar, Farah Naz, additional, Qureshi, Sonia, additional, Rabe, Ingrid B., additional, Rahman, Atif, additional, Rahmati, Elham, additional, Raizes, Elliot, additional, Ramalho-Ortigao, Marcelo, additional, Raoult, Didier, additional, Rein, Michael F., additional, Retik, Alan B., additional, Reynes, Jean-Marc, additional, Rhatigan, Joseph J., additional, Rickard, Jennifer, additional, Riddle, Mark S., additional, Rimoin, Anne W., additional, Riviello, Robert, additional, Robert, Leon L., additional, Rodrigues, Ema G., additional, Rodriguez, Rubens, additional, Ronald, Allan R., additional, Rosenthal, Benjamin M., additional, Rosmarin, David, additional, Ryan, Edward T., additional, Saavedra, Arturo, additional, Schiaffino, Francesca, additional, Schumacher, Sandra K., additional, Sejvar, James J., additional, Sethi, Aisha, additional, Seung, Kwonjune J., additional, Seydel, Karl B., additional, Shah, Melisa M., additional, Shakoor, Sadia, additional, Shankar, Anuraj H., additional, Sharp, Trueman W., additional, Shin, Sonya S., additional, Shirley, Debbie-Ann, additional, Silachamroon, Udomsak, additional, Smith, Catherine C., additional, Snelling, Thomas L., additional, Solomon, Tom, additional, Staat, Mary Allen, additional, Staples, J. Erin, additional, Steiger, Samantha N., additional, Stewart, Robert C., additional, Stich, August, additional, Strickman, Daniel, additional, Suh, Kathryn N., additional, Suhrbier, Andreas, additional, Sutcliffe, Catherine G., additional, Tappe, Dennis, additional, Taylor, Terrie E., additional, Thanh, Nguyen Tat, additional, Thanh, Nguyen Thi, additional, Thwaites, C. Louise, additional, Thwaites, Guy E., additional, Tiwari, Tejpratap S.P., additional, Tsenempi, Xenia A., additional, Turner, Angus W., additional, van den Broek, Nynke R., additional, van Doorn, H. Rogier, additional, Van Sickels, Nicholas J., additional, Vannier, Edouard, additional, Varda, Briony K., additional, Vasconcelos, Pedro F.C., additional, Vega-López, Francisco, additional, Vietri, Nicholas J., additional, Vinetz, Joseph M., additional, Visvesvara, Govinda S., additional, Vyas, Keyur S., additional, Walsh, Thomas J., additional, Wansbrough-Jones, Mark H., additional, Warraich, Haider J., additional, Warrell, David A., additional, Warrell, Mary J., additional, Watt, George, additional, Wattanagoon, Yupaporn, additional, Watthanakulpanich, Dorn, additional, Weaver, Scott C., additional, Weil, Ana A., additional, Weiss, Louis M., additional, White, Nicholas J., additional, Whitty, Christopher J.M., additional, Wilson, Mary E., additional, Xavier, Ramnik J., additional, Xiao, Lihua, additional, Yoon, In-Kyu, additional, Yu, Hongjie, additional, and Zaidi, Anita K.M., additional
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- 2020
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3. Pathogenic and Opportunistic Free-Living Ameba Infections
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Cope, Jennifer R., primary, Ali, Ibne K., additional, and Visvesvara, Govinda S., additional
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- 2020
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4. The Epidemiology and Clinical Features of Non-Keratitis Acanthamoeba Infections in the United States, 1956–2020
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Haston, Julia C, primary, O’Laughlin, Kevin, additional, Matteson, Kelsey, additional, Roy, Shantanu, additional, Qvarnstrom, Yvonne, additional, Ali, Ibne K M, additional, and Cope, Jennifer R, additional
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- 2023
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5. Acanthamoeba granulomatous amoebic encephalitis after pediatric hematopoietic stem cell transplant
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Coven, Scott L., Song, Eunkyung, Steward, Sarah, Pierson, Christopher R., Cope, Jennifer R., Ali, Ibne K., Ardura, Monica I., Hall, Mark W., Chung, Melissa G., and Bajwa, Rajinder P. S.
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- 2017
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6. Primary Amebic Meningoencephalitis: What Have We Learned in the Last 5 Years?
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Cope, Jennifer R. and Ali, Ibne K.
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- 2016
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7. 107 - Pathogenic and Opportunistic Free-Living Ameba Infections
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Cope, Jennifer R., Ali, Ibne K., and Visvesvara, Govinda S.
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- 2020
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8. USE OF A HUMAN INDIRECT IMMUNOFLUORESCENCE ANTIBODY ASSAY FOR BALAMUTHIA MANDRILLARIS IN A GROUP OF CAPTIVE NORTHWEST BORNEAN ORANGUTANS (PONGO PYGMAEUS PYGMAEUS)
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Ferris, Rachel L., primary, Ali, Ibne K., additional, and West, Gary, additional
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- 2021
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9. A rare cause of left-sided weakness in an elderly woman: amoebic encephalitis
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Pan, Daniel, primary, Bridges, Leslie R, additional, du Parcq, John, additional, Mahadeva, Ula, additional, Roy, Shantanu, additional, Ali, Ibne K M, additional, Cosgrove, Catherine A, additional, Chiodini, Peter L, additional, and Zhang, Liqun, additional
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- 2020
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10. Pathology in Practice
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Suepaul, Rod B., primary, Frontera-Acevedo, Karelma, additional, Gyan, Lana A., additional, Ramdoo, Charneth, additional, Ali, Ibne K., additional, Roy, Shantanu, additional, Rajh, Stacy, additional, and Gary, Joy, additional
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- 2020
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11. A case of primary amebic meningoencephalitis caused by Naegleria fowleri in Bangladesh
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Sazzad, Hossain M. S., primary, Luby, Stephen P., additional, Sejvar, James, additional, Rahman, Mahmudur, additional, Gurley, Emily S., additional, Hill, Vincent, additional, Murphy, Jennifer L., additional, Roy, Shantanu, additional, Cope, Jennifer R., additional, and Ali, Ibne K. M., additional
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- 2019
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12. Primary amebic meningoencephalomyelitis caused by Naegleria fowleri in a south-central black rhinoceros (Diceros bicornis minor)
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Yaw, Taylor J., primary, O'Neil, Pat, additional, Gary, Joy M., additional, Ali, Ibne K., additional, Cowart, Jerry R., additional, Wallace, Roberta S., additional, and Estep, J. Scot, additional
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- 2019
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13. Case Report: Cervicovaginal Co-Colonization with Entamoeba gingivalis and Entamoeba polecki in Association with an Intrauterine Device
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Bradbury, Richard S., primary, Roy, Shantanu, additional, Ali, Ibne K., additional, Morrison, Jasmin R., additional, Waldner, Dale, additional, Hebbeln, Katherine, additional, Aldous, Wade, additional, Jepson, Ryan, additional, Delavan, Haleigh R., additional, Ndubuisi, MacKevin, additional, and Bishop, Henry S., additional
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- 2019
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14. The Epidemiology and Clinical Features of Balamuthia mandrillaris Disease in the United States, 1974–2016
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Cope, Jennifer R, primary, Landa, Janet, additional, Nethercut, Hannah, additional, Collier, Sarah A, additional, Glaser, Carol, additional, Moser, Melanie, additional, Puttagunta, Raghuveer, additional, Yoder, Jonathan S, additional, Ali, Ibne K, additional, and Roy, Sharon L, additional
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- 2018
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15. Primary Amebic Meningoencephalitis: What Have We Learned in the Last Five Years?
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Cope, Jennifer R. and Ali, Ibne K.
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parasitic diseases ,Article - Abstract
Primary amebic meningoencephalitis (PAM) is a devastating infection of the brain caused by the thermophilic free-living ameba, Naegleria fowleri. Infection can occur when water containing the ameba enters the body through the nose, usually during recreational water activities such as swimming or diving. Historically, in the United States, cases were mostly reported from the warmer southern-tier states. In the last five years, several notable changes have been documented in PAM epidemiology including a northward expansion of infections and new types of water exposures. The recent reports of two PAM survivors provide hope for improved outcomes with early diagnosis and aggressive treatment. Advanced molecular laboratory tools such as genome sequencing might provide more insight into the pathogenicity of Naegleria fowleri. Clinicians treating patients with meningitis and warm freshwater exposure are encouraged to consider PAM in their differential diagnoses.
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- 2016
16. Acanthamoebagranulomatous amoebic encephalitis after pediatric hematopoietic stem cell transplant
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Coven, Scott L., primary, Song, Eunkyung, additional, Steward, Sarah, additional, Pierson, Christopher R., additional, Cope, Jennifer R., additional, Ali, Ibne K., additional, Ardura, Monica I., additional, Hall, Mark W., additional, Chung, Melissa G., additional, and Bajwa, Rajinder P. S., additional
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- 2017
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17. Epidemiology and Clinical Features of Balamuthia mandrillaris Disease in the United States, 1974–2016.
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Cope, Jennifer R, Landa, Janet, Nethercut, Hannah, Collier, Sarah A, Glaser, Carol, Moser, Melanie, Puttagunta, Raghuveer, Yoder, Jonathan S, Ali, Ibne K, and Roy, Sharon L
- Subjects
AMEBIASIS treatment ,AMEBIASIS ,REPORTING of diseases ,ENCEPHALITIS ,ETHNIC groups ,HISPANIC Americans ,PROFESSIONS ,SOILS ,SYMPTOMS ,TREATMENT effectiveness ,EARLY diagnosis ,DATA analysis software ,DESCRIPTIVE statistics - Abstract
Background Balamuthia mandrillaris is a free-living ameba that causes rare, nearly always fatal disease in humans and animals worldwide. B. mandrillaris has been isolated from soil, dust, and water. Initial entry of Balamuthia into the body is likely via the skin or lungs. To date, only individual case reports and small case series have been published. Methods The Centers for Disease Control and Prevention (CDC) maintains a free-living ameba (FLA) registry and laboratory. To be entered into the registry, a Balamuthia case must be laboratory-confirmed. Several sources were used to complete entries in the registry, including case report forms, CDC laboratory results, published case reports, and media information. SAS© version 9.3 software was used to calculate descriptive statistics and frequencies. Results We identified 109 case reports of Balamuthia disease between 1974 and 2016. Most (99%) had encephalitis. The median age was 36 years (range 4 months to 91 years). Males accounted for 68% of the case patients. California had the highest number of case reports, followed by Texas and Arizona. Hispanics constituted 55% for those with documented ethnicity. Exposure to soil was commonly reported. Among those with a known outcome, 90% of patients died. Conclusions Balamuthia disease in the United States is characterized by a highly fatal encephalitis that affects patients of all ages. Hispanics were disproportionately affected. The southwest region of the United States reported the most cases. Clinician awareness of Balamuthia as a cause of encephalitis might lead to earlier diagnosis and initiation of treatment, resulting in better outcomes. [ABSTRACT FROM AUTHOR]
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- 2019
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18. Species-Specific Immunodetection of an Entamoeba histolytica Cyst Wall Protein
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Spadafora, Lauren J., primary, Kearney, Moira R., additional, Siddique, Abdullah, additional, Ali, Ibne K., additional, Gilchrist, Carol A., additional, Arju, Tuhinur, additional, Hoffstrom, Benjamin, additional, Nguyen, Felicia K., additional, Petri, William A., additional, Haque, Rashidul, additional, and Cangelosi, Gerard A., additional
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- 2016
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19. Toward Low-Cost Affinity Reagents: Lyophilized Yeast-scFv Probes Specific for Pathogen Antigens
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Gray, Sean A., primary, Weigel, Kris M., additional, Ali, Ibne K. M., additional, Lakey, Annie A., additional, Capalungan, Jeremy, additional, Domingo, Gonzalo J., additional, and Cangelosi, Gerard A., additional
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- 2012
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20. Toward Low-Cost Affinity Reagents: Lyophilized YeastscFv Probes Specific for Pathogen Antigens.
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Gray, Sean A., Weigel, Kris M., Ali, Ibne K. M., Lakey, Annie A., Capalungan, Jeremy, Domingo, Gonzalo J., and Cangelosi, Gerard A.
- Subjects
ANTIGENS ,MONOCLONAL antibodies ,YEAST ,RECOMBINANT molecules ,PROTEINS - Abstract
The generation of affinity reagents, usually monoclonal antibodies, remains a critical bottleneck in biomedical research and diagnostic test development. Recombinant antibody-like proteins such as scFv have yet to replace traditional monoclonal antibodies in antigen detection applications, in large part because of poor performance of scFv in solution. To address this limitation, we have developed assays that use whole yeast cells expressing scFv on their surfaces (yeast-scFv) in place of soluble purified scFv or traditional monoclonal antibodies. In this study, a nonimmune library of human scFv displayed on the surfaces of yeast cells was screened for clones that bind to recombinant cyst proteins of Entamoeba histolytica, an enteric pathogen of humans. Selected yeast-scFv clones were stabilized by lyophilization and used in detection assay formats in which the yeast-scFv served as solid support-bound monoclonal antibodies. Specific binding of antigen to the yeast-scFv was detected by staining with rabbit polyclonal antibodies. In flow cytometry-based assays, lyophilized yeastscFv reagents retained full binding activity and specificity for their cognate antigens after 4 weeks of storage at room temperature in the absence of desiccants or stabilizers. Because flow cytometry is not available to all potential assay users, an immunofluorescence assay was also developed that detects antigen with similar sensitivity and specificity. Antigenspecific whole-cell yeast-scFv reagents can be selected from nonimmune libraries in 2-3 weeks, produced in vast quantities, and packaged in lyophilized form for extended shelf life. Lyophilized yeast-scFv show promise as low cost, renewable alternatives to monoclonal antibodies for diagnosis and research. [ABSTRACT FROM AUTHOR]
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- 2012
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21. The Epidemiology and Clinical Features of Balamuthia mandrillaris Disease in the United States, 1974-2016.
- Author
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Cope JR, Landa J, Nethercut H, Collier SA, Glaser C, Moser M, Puttagunta R, Yoder JS, Ali IK, and Roy SL
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Amebiasis mortality, Amebiasis physiopathology, Central Nervous System Protozoal Infections mortality, Central Nervous System Protozoal Infections physiopathology, Child, Child, Preschool, Female, Humans, Infant, Infectious Encephalitis mortality, Infectious Encephalitis physiopathology, Male, Middle Aged, Sequence Analysis, DNA, United States epidemiology, Young Adult, Amebiasis epidemiology, Balamuthia mandrillaris pathogenicity, Central Nervous System Protozoal Infections epidemiology, Infectious Encephalitis epidemiology, Registries
- Abstract
Background: Balamuthia mandrillaris is a free-living ameba that causes rare, nearly always fatal disease in humans and animals worldwide. B. mandrillaris has been isolated from soil, dust, and water. Initial entry of Balamuthia into the body is likely via the skin or lungs. To date, only individual case reports and small case series have been published., Methods: The Centers for Disease Control and Prevention (CDC) maintains a free-living ameba (FLA) registry and laboratory. To be entered into the registry, a Balamuthia case must be laboratory-confirmed. Several sources were used to complete entries in the registry, including case report forms, CDC laboratory results, published case reports, and media information. SAS© version 9.3 software was used to calculate descriptive statistics and frequencies., Results: We identified 109 case reports of Balamuthia disease between 1974 and 2016. Most (99%) had encephalitis. The median age was 36 years (range 4 months to 91 years). Males accounted for 68% of the case patients. California had the highest number of case reports, followed by Texas and Arizona. Hispanics constituted 55% for those with documented ethnicity. Exposure to soil was commonly reported. Among those with a known outcome, 90% of patients died., Conclusions: Balamuthia disease in the United States is characterized by a highly fatal encephalitis that affects patients of all ages. Hispanics were disproportionately affected. The southwest region of the United States reported the most cases. Clinician awareness of Balamuthia as a cause of encephalitis might lead to earlier diagnosis and initiation of treatment, resulting in better outcomes., (Published by Oxford University Press for the Infectious Diseases Society of America 2018. This work is written by (a) US Government employee(s) and is in the public domain in the US.)
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- 2019
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22. Diagnosing Balamuthia mandrillaris Encephalitis With Metagenomic Deep Sequencing.
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Wilson MR, Shanbhag NM, Reid MJ, Singhal NS, Gelfand JM, Sample HA, Benkli B, O'Donovan BD, Ali IK, Keating MK, Dunnebacke TH, Wood MD, Bollen A, and DeRisi JL
- Subjects
- Aged, Amebiasis cerebrospinal fluid, Animals, Brain microbiology, DNA, Protozoan genetics, Female, Genomics, Humans, Meningoencephalitis cerebrospinal fluid, Polymerase Chain Reaction, Vitreous Body microbiology, Amebiasis diagnosis, Amebiasis genetics, Balamuthia mandrillaris genetics, Meningoencephalitis diagnosis, Meningoencephalitis genetics, Sequence Analysis, RNA methods
- Abstract
Objective: Identification of a particular cause of meningoencephalitis can be challenging owing to the myriad bacteria, viruses, fungi, and parasites that can produce overlapping clinical phenotypes, frequently delaying diagnosis and therapy. Metagenomic deep sequencing (MDS) approaches to infectious disease diagnostics are known for their ability to identify unusual or novel viruses and thus are well suited for investigating possible etiologies of meningoencephalitis., Methods: We present the case of a 74-year-old woman with endophthalmitis followed by meningoencephalitis. MDS of her cerebrospinal fluid (CSF) was performed to identify an infectious agent., Results: Sequences aligning to Balamuthia mandrillaris ribosomal RNA genes were identified in the CSF by MDS. Polymerase chain reaction subsequently confirmed the presence of B. mandrillaris in CSF, brain tissue, and vitreous fluid from the patient's infected eye. B. mandrillaris serology and immunohistochemistry for free-living amoebas on the brain biopsy tissue were positive., Interpretation: The diagnosis was made using MDS after the patient had been hospitalized for several weeks and subjected to costly and invasive testing. MDS is a powerful diagnostic tool with the potential for rapid and unbiased pathogen identification leading to early therapeutic targeting., (© 2015 The Authors Annals of Neurology published by Wiley Periodicals, Inc. on behalf of American Neurological Association.)
- Published
- 2015
- Full Text
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