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In this study, we prepared a potential catalyst as an electrode modifier for electrolytic water splitting. In the preparation step, the amine was decorated with copper-phosphorus. It was immobilized over the silica surface, and the surface was engineered using N -(3-(trimethoxysilyl) propyl)ethylenediamine for the synthesis of the catalysts (AS). The morphological and structural aspects of the catalyst (AFS-Cu-P) were determined using FE-SEM/EDAX, FTIR, elemental analysis, BET, TGA, and XPS. The catalyst's efficacy for the oxygen evolution reaction (OER) was assessed in an alkaline medium with and without hydrazine. The hydrazine oxidation reaction enhanced the sluggish OER and facilitated water splitting. Detailed electrochemical measurements confirmed an increase in the kinetics of the process and a reduction in the activation energy needed to complete the process. The Tafel slopes, charge transfer coefficients, exchange-specific current densities, apparent rate constants, and diffusion coefficients are provided along with their respective values. The results showed that the presence of Cu and CNT is crucial in the conversion process., Competing Interests: The authors declare no conflict of interest., (This journal is © The Royal Society of Chemistry.)

Background: Sudden death is defined as an unexpected death occurring with no observed antecedent clinical signs., Aim: The current study was performed to notice the tangible causes of sudden death among 51 out of 340 she-camels on a private farm in the eastern region of El Khafgi, Saudi Arabia., Methods: A retrospective cohort study design was conducted to investigate the sudden death of camels through microscopic examination of fecal matter to identify the gastrointestinal parasites, analysis of whole blood thin films to diagnose blood parasites, blood culturing to recognize bacterial infection as Pasteurella multicida , and macroscopic postmortem examination to identify the gastrointestinal adult worm. The quantity and composition of feed were also analyzed. Afterward, a commercial multiscreen Ag-ELISA kit technique determined the toxins of Clostridium perfringens ( C. perfringens )., Results: The results revealed that the incidence rate of sudden death was 15%. The sudden death occurred due to C. perfringens enterotoxins detected in the rumen, intestinal content, and intestinal wall. The enterotoxins and Alpha toxins were noticed, but the other toxin types, including Beta and Epsilon, could not be detected. All C. perfringens toxins were discovered to be negative in fecal matter. A significant association was reported between sudden death, she-camels age, and feeding habits as risk factors ( p = 0.020 and 0.028, respectively). Risk factor assessment by relative risk (RR) revealed that the odds of RR of sudden death occurring among she-camels aged over two years were higher than those less than two years (2.24 CI 95%, 1.093-4.591). Furthermore, the odds RR of sudden death occurring due to exposure of she-camels to a concentrated ration of 18% were higher twice than those not exposed (2.346 CI 95%, 1.039-5.296)., Conclusion: Clostridium perfringens enterotoxaemia should be listed as a cause of sudden death in camels and the alteration in diet with 18% concentration feed changes the intestinal environment, which leads to C. perfringens proliferating and yielding potent toxins. More observations and interferences like regular immunization are recommended to reduce the disease and increase the awareness of the farmers of the importance of risk factors., Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this manuscript.

Identifying indolent and aggressive prostate cancers is a critical problem for optimal treatment. The existing approaches of prostate cancer detection are facing challenges as the techniques rely on ground truth labels with limited accuracy, and histological similarity, and do not consider the disease pathology characteristics, and indefinite differences in appearance between the cancerous and healthy tissue lead to many false positive and false negative interpretations. Hence, this research introduces a comprehensive framework designed to achieve accurate identification and localization of prostate cancers, irrespective of their aggressiveness. This is accomplished through the utilization of a sophisticated multilevel bidirectional long short-term memory (Bi-LSTM) model. The pre-processed images are subjected to multilevel feature map-based U-Net segmentation, bolstered by ResNet-101 and a channel-based attention module that improves the performance. Subsequently, segmented images undergo feature extraction, encompassing various feature types, including statistical features, a global hybrid-based feature map, and a ResNet-101 feature map that enhances the detection accuracy. The extracted features are fed to the multilevel Bi-LSTM model, further optimized through channel and spatial attention mechanisms that offer the effective localization and recognition of complex structures of cancer. Further, the framework represents a promising approach for enhancing the diagnosis and localization of prostate cancers, encompassing both indolent and aggressive cases. Rigorous testing on a distinct dataset demonstrates the model's effectiveness, with performance evaluated through key metrics which are reported as 96.72%, 96.17%, and 96.17% for accuracy, sensitivity, and specificity respectively utilizing the dataset 1. For dataset 2, the model achieves the accuracy, sensitivity, and specificity values of 94.41%, 93.10%, and 94.96% respectively. These results surpass the efficiency of alternative methods., (© 2024. The Author(s) under exclusive licence to Society for Imaging Informatics in Medicine.)

Breast cancer is one of the most often diagnosed cancers in women, and identifying breast cancer histological images is an essential challenge in automated pathology analysis. According to research, the global BrC is around 12% of all cancer cases. Furthermore, around 25% of women suffer from BrC. Consequently, the prediction of BrC depends critically on the quick and precise processing of imaging data. The primary reason deep learning models are used in breast cancer detection is that they can produce findings more quickly and accurately than current machine learning-based techniques. Using a BreakHis dataset, we demonstrated in this work the viability of automatically identifying and classifying BrC. The first stage is pre-processing, which employs an Adaptive Switching Modified Decision Based Unsymmetrical Trimmed Median Filter (ASMDBUTMF) to remove high-density noise. After the image has been pre-processed, it is segmented using the Thresholding Level set approach. Next, we propose a hybrid chaotic sand cat optimization technique, together with the Remora Optimization Algorithm (ROA) for feature selection. The suggested strategy facilitates the acquisition of precise functionality attributes, hence simplifying the detection procedure. Additionally, it aids in resolving problems pertaining to global optimization. Following the selection, the best characteristics proceed to the categorization procedure. A DL classifier called the Conditional Variation Autoencoder is used to discriminate between cancerous and benign tumors while categorizing them. Consequently, a classification accuracy of 99.4%, Precision of 99.2%, Recall of 99.1%, F- score of 99%, Specificity of 99.14%, FDR of 0.54, FNR of 0.001, FPR of 0.002, MCC of 0.98 and NPV of 0.99 were obtained using the proposed approach. Furthermore, compared to other research using the current BreakHis dataset, the results of our research are more desirable., Competing Interests: The author have declared that no competing interests exist., (Copyright: © 2024 Afnan M. Alhassan. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Until the outbreak of the coronavirus disease 2019 (COVID-19) pandemic, developing countries, especially countries in the African continent, battled with the impact of climate change on the food value-chain systems and general livelihood. In this study, we discuss climate change concerns post-COVID-19 and argue that the outbreak of the COVID-19 pandemic has exacerbated the vulnerabilities of most developing and emerging economies. This has heightened political tensions and unrest among such developing nations. We suggest enhancement and intensification of efficient and effective locally engineered adaptation strategies in the post-COVID-19 era for countries that have been susceptible to the impact of climate change and other recent shocks.

Background: Neonatal sepsis is a clinical emergency that requires sound evaluation supported by accurate laboratory analysis and timely clinical intervention for its management. This study, therefore, was conducted to identify bacteria causing neonatal sepsis and their susceptibility to the commonly prescribed antibiotic at the Neonatal Intensive Care Unit of a tertiary health care facility in the Northern Region of Ghana., Methods: Neonatal biodata were collected from patient folders, after which identification, isolation, and susceptibility of isolated bacteria to prescribed anti-bacterial (Kirby-Bauer disk diffusion method) were carried out on single venipuncture blood samples aseptically drawn from 275 neonates clinically diagnosed with sepsis., Results: 275 neonates took part in the study, of which 218 (79.3%) presented with early-onset sepsis (EOS) and 57 (20.7%) with late-onset sepsis (LOS). The laboratory results confirmed a septicemia prevalence of 70.3% among neonates clinically diagnosed with sepsis. Preterm delivery ( P  = .01), hypothermia ( P  = .001), and delivery at the tertiary healthcare facility were significantly associated with EOS ( P  < .000), while low birth weight ( P  = .012), duration of hospital stay ( P  = .001), and delivery at the tertiary healthcare facility ( P  < .000) were found to be significantly associated with LOS. Gram-positive cocci constituted 54.9% (107), with Gram-negative constituting 45.1% (88) of all the bacteria isolates. Coagulase-negative staphylococcus (CoNS) 70.1% (75) and Klebsiella species 39.8% (35) were the dominant Gram-positive and Gram-negative isolates, respectively. 57.8% and 55.8% of CoNS isolates were susceptible to ampicillin and amoxicillin/clavulanic acid, respectively. 93.5% of CoNS and all the isolated Staphylococcus aureus and Klebsiella species were susceptible to amikacin., Conclusions: Coagulase-negative staphylococcus (CoNS) and Klebsiella species were the predominant Gram-positive and negative sepsis-causing agents at the NICU, respectively. Amikacin exhibited the highest sensitivity to Gram-positive and negative causative agents, making it a strong candidate for consideration in the facility's empirical treatment of neonatal sepsis., Competing Interests: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2023.)

Background and Aim: Neonatal sepsis is a systemic inflammatory response to infection during the first 4 weeks of an infant's life. It is a significant cause of neonatal morbidity and mortality in low- and middle-income countries. This study aimed to determine the predictors of the onset of sepsis at the Neonatal Intensive Care Unit of the Tamale Teaching Hospital, Ghana., Methods: A cross-sectional study was conducted among 275 mothers and their singleton neonates diagnosed clinically with sepsis. A univariate and multivariate logistic regression analysis adjusted for maternal occupational status was performed to determine the maternal and neonatal predictors of early-onset (EOS) and late-onset sepsis (LOS), respectively., Results: Single motherhood (AOR = 1.882, 95% CI = 0.926-3.822, p = .08) and home delivery (AOR = 3.667, 95% CI = 0.584-23.026, p  = .17) were predictors of EOS, with single motherhood being the predictor for LOS (AOR = 2.906, 95% CI = 0.715-11.805, p  = .14) in a univariate analysis. When maternal occupation was adjusted for in a multivariate analysis, single mother (AOR = 2.167, 95% CI = 1.010-4.648, p  = .04) was the main predictor of EOS, with low neonatal birth weight being the main predictor of LOS (AOR = 0.193, 95% CI = 0.038-0.971, p  = .04)., Conclusion: Maternal marital status is a significant predictor of both EOS and LOS, with predictors of EOS being lower gestational age and low birth weight, while for LOS, low birth weight is the main predictor. Findings from this study can serve as a commencement point for developing predictive models for the onset of sepsis in neonates in the study facility., Competing Interests: The authors declare no conflict of interest., (© 2023 The Authors. Health Science Reports published by Wiley Periodicals LLC.)

Small private irrigation (SPI) is a farmer-initiated irrigation which has the potential to increase the contribution of the overall irrigation sector to global food security. However, there is no much information about these systems for effective policies for regulation. This study compared the resource use productivities and environmental impacts of SPI systems to those of a government-led irrigation scheme (GIS) in Northern Ghana. The results showed that land productivity was higher in the SPI than in the GIS. Productivity per unit cultivated area was 2571.00 US$/ha under SPI while that of the GIS was 676.00 US$/ha. Output per unit command area was also two times higher in the SPI than in the GIS; that is 2571.00 US$/ha and 1113.00 US$/ha for SPI and GIS respectively. For water productivity, output per unit irrigation supply was 0.33 US$/m 3 and 0.08US$/m 3 for SPI and GIS respectively while output per unit water consumed by ET was 0.60 US$/m 3 for SPI and 0.06 US$/m 3 for the GIS. The results implied that the SPI schemes performed better in land and water productivities compared with the GIS which is attributed to higher yields and the selection of high valued crops by farmers under SPI. However, both irrigation system types at the time of this study did not cause significant deterioration to the water bodies and surrounding environment as the biochemical oxygen demand (BOD) values of nearby water bodies were less than 3.0-5.0 mg/l, which is considered as acceptable levels for drinking water by World Health Organisation (WHO) while salinity levels were also within acceptable limits (<750 μS/cm). With appropriate policies to regulate and provide support systems to the SPI, these systems may increase the overall agricultural productivity and improve job creation for the teeming unemployed youth and women in the savannah agroecological zone of Ghana., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

In this work, the solvothermal solidification method has been used to be prepared as a homogenous CuSn-organic nano-composite (CuSn-OC) to use as a catalyst for alkaline water electrolysis for cost-effective H 2 generation. FT-IR, XRD, and SEM techniques were used to characterize the CuSn-OC which confirmed the formation of CuSn-OC with a terephthalic acid linker as well as Cu-OC and Sn-OC. The electrochemical investigation of CuSn-OC onto a glassy carbon electrode (GCE) was evaluated using the cyclic voltammetry (CV) method in 0.1 M KOH at room temperature. The thermal stability was examined using TGA methods, and the Cu-OC recorded a 91.4% weight loss after 800 °C whereas the Sn-OC and CuSn-OC recorded 16.5 and 62.4%, respectively. The results of the electroactive surface area (ECSA) were 0.5, 0.42, and 0.33 m 2 g -1 for the CuSn-OC, Cu-OC, and Sn-OC, respectively, and the onset potentials for HER were -420, -900, and -430 mV vs. the RHE for the Cu-OC, Sn-OC, and CuSn-OC, respectively. LSV was used to evaluate the electrode kinetics, and the Tafel slope for the bimetallic catalyst CuSn-OC was 190 mV dec -1 , which was less than for both the monometallic catalysts, Cu-OC and Sn-OC, while the overpotential was -0.7 vs. the RHE at a current density of -10 mA cm -2 .

Background: Coronavirus disease 2019 (COVID-19) has emerged as an important cause of morbidity and mortality worldwide. The aim of this study is to identify the clinical predictors of mortality among patients with COVID-19 pneumonia during first and second waves in a treatment center in northwestern Nigeria., Methods: This was a retrospective cohort study of 195 patients hospitalized with COVID-19 between April 2020 to March 2021 at a designated COVID-19 isolation center in Kano State, Northwest Nigeria. Data were summarized using frequencies and percentages. Unadjusted odds ratios and 95% confidence intervals and p-values were obtained. To determine independent determinants of mortality, we performed a stepwise multivariate logistic regression model., Results: Of 195 patients studied, 21(10.77%) patients died. Males comprised 158 (81.03%) of the study population. In the adjusted stepwise logistic regression analysis, age>64 years (OR = 9.476, 95% CI: 2.181-41.165), second wave of the pandemic (OR = 49.340, 95% CI:6.222-391.247), cardiac complications (OR = 24.984, 95% CI: 3.618-172.508), hypertension (OR = 5.831, 95% CI:1.413-24.065) and lowest systolic blood pressure while on admission greater than or equal to 90mmHg were independent predictors of mortality (OR = 0.111, 95%CI: 0.021-0.581)., Conclusion: Strategies targeted to prioritize needed care to patients with identified factors that predict mortality might improve patient outcome., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Dayyab et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Background: Hepatocellular carcinoma (HCC) is associated with a high mortality rate due to early recurrence and its metastasis features. To this day, effective treatment options for metastatic HCC remain a major challenge to patient treatment. Flavokawain B (FKB) is a naturally occurring chalcone molecule capable of providing effective therapy against this life-threatening disease., Objective: This study investigated the anti-metastatic effects of FKB on the growth and development of metastatic HCC., Methods: HepG2 cells were used in this study and a neutral red assay was performed to determine the IC 50 value of FKB. Cell scratch and exclusion zone assays were performed to assess the rate of cell migration and invasion. Relative mRNA levels of UCK2, STAT3, VEGF and HIF-1α genes were quantified using RT-qPCR., Results: FKB inhibited the proliferation of HepG2 cells at an IC 50 value of 28 μM after 72 h of incubation. Its cytotoxic effect was confirmed to induce apoptosis through the phase-contrast inverted microscope. Cell migration and invasion were significantly inhibited at 7, 14, and 28 μM of FKB as compared to untreated cells. The inhibition in the cell migration significantly increased with the increasing concentrations of the bioactive compound. The relative expression levels of the UCK2 gene and its downstream genes, STAT3, VEGF and HIF-1α , were significantly downregulated after 72 h exposure to FKB treatment., Conclusion: Our data suggest that FKB inhibited HepG2 proliferation and further suppressed its metastasis partly by regulating the STAT3/Hif-1α/VEGF signalling pathway. FKB could be a potential alternative and viable strategy against HCC., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Background and Aim: Newcastle disease (ND), a major constraint to poultry production worldwide, is a highly contagious disease of many species of domestic, exotic, and wild birds caused by ND virus (NDV). Epidemiological studies are lacking regarding ND in village chickens, including the traditional and intensive production systems used in Sudan. However, it is necessary to develop appropriate strategies to control the disease. Therefore, this study aimed to estimate the flock- and bird-level seroprevalence of NDV in backyard chickens in West Kordofan State, Sudan, and to identify the risk factors associated with ND in the study area., Materials and Methods: The seroprevalence of the circulating NDV and bird-level risk factors associated with ND was determined in backyard chickens from March to October 2017, in six villages (Alnowara, Alleait, Geibaish, Baiad, Sougoh, and Alnuhoud) in the Geibaish and Elnuhoud localities of West Kordofan State., Results: Using the hemagglutination-inhibition test, the bird- and flock-level seroprevalences of antibodies to NDV were estimated as 20.6% (78/378) and 45% (18/40), respectively. Bird-level NDV seropositivity in backyard chickens was significantly associated with the reason for raising chickens, type of housing, contact with neighboring poultry, contact with wild birds, and chicken mortality caused by infectious diseases (p ≤ 0.05)., Conclusion: This study indicated that NDV is circulating in backyard chickens and may act as a potential source of infection for other birds and thus persistence of ND among local traditionally managed chickens in the areas of West Kordofan State. Risk factors contributing to ND occurrence are important for designing appropriate prevention and control strategies., Competing Interests: The authors declare that they have no competing interests., (Copyright: © Hussein, et al.)

Denitrifying bacteria produce and utilize nitrous oxide (N 2 O), a potent greenhouse gas. However, there is little information on how organic fertilization treatments affect the denitrifying communities and N 2 O emissions in the semi-arid Loess Plateau. Here, we evaluated how the denitrifying communities are responsible for potential denitrification activity (PDA) and N 2 O emissions. A field experiment was conducted with five fertilization treatments, including no fertilization (CK), mineral fertilizer (MF), mineral fertilizer plus commercial organic fertilizer (MOF), commercial organic fertilizer (OFP), and maize straw (MSP). Our result showed that soil pH, soil organic carbon (SOC), and dissolved organic nitrogen (DON) were significantly increased under MSP treatment compared to MF treatment, while nitrate nitrogen (NO 3 - -N) followed the opposite trend. Organic fertilization treatments (MOF, OFP, and MSP treatments) significantly increased the abundance and diversity of nirS - and nosZ -harboring denitrifiers, and modified the community structure compared to CK treatment. The identified potential keystone taxa within the denitrifying bacterial networks belonged to the distinct genera. Denitrification potentials were significantly positively correlated with the abundance of nirS -harboring denitrifiers, rather than that of nirK - and nosZ -harboring denitrifiers. Random forest modeling and structural equation modeling consistently determined that the abundance, community composition, and network module I of nirS -harboring denitrifiers may contribute significantly to PDA and N 2 O emissions. Collectively, our findings highlight the ecological importance of the denitrifying communities in mediating denitrification potentials and the stimulatory impact of organic fertilization treatments on nitrogen dynamics in the semi-arid Loess Plateau., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Fudjoe, Li, Jiang, Alhassan, Xie, Anwar, Wang and Xie.)

Vernonia glaberrima leaves are traditionally used to alleviate bodily pain, skin cancer, and other skin-related disorders. The purpose of the study was to investigate the acute and sub-acute toxicity of 5-methylcoumarin-4β-glucoside, a promising chemotherapeutic agent against colon cancer isolated from the leaves of Vernonia glaberrima . 5-methylcoumarin-4β-glucoside was isolated from the methanol leaf extract of Vernonia glaberrima following a previously described method. The acute toxicity study involved a two-phase 24 h observation for signs of mortality and toxicity following single oral dose administration of the isolated compound. For the sub-acute study, four groups of mice, averagely aged eight weeks, were administered graded doses of the compound (250, 500 and 1000 mg/kg) or vehicle for 28 days. On the 29th day, the mice were fasted, anesthetized, euthanized, then their blood and tissues were harvested for hematological, biochemical and histopathological evaluations. There were no signs of mortality or moribund status with an increasing dose of up to 5000 mg/kg over a 24 h period in the acute study. Also, there was no evidence of toxicity on the biochemical or hematopoietic systems in the sub-acute study (p < 0.05). At the dose of 1000 mg/kg, the mice showed some distorted histology with no corresponding alterations in serum biochemicals. Overall, the results showed that 5-methylcoumarin-4β-glucoside at dosages up to 500 mg/kg is tolerable in mice., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 The Authors.)

Cancer is a rapidly growing non-communicable disease worldwide that is responsible for high mortality rates, which account for 9.6 million death in 2018. Dihydroartemisinin (DHA) is an active metabolite of artemisinin, an active principle present in the Chinese medicinal plant Artemisia annua used for malaria treatment. Dihydroartemisinin possesses remarkable and selective anticancer properties however the underlying mechanism of the antitumor effects of DHA from the structural point of view is still not yet elucidated. In the present study, we employed molecular docking simulation techniques using Autodock suits to access the binding properties of dihydroartemisinin to multiple protein targets implicated in cancer pathogenesis. Its potential targets with comprehensive pharmacophore were predicted using a PharmMapper database. The co-crystallised structures of the protein were obtained from a Protein Data Bank and prepared for molecular docking simulation. Out of the 24 selected protein targets, DHA has shown about 29% excellent binding to the targets compared to their co-crystallised ligand. Additionally, 75% of the targets identified for dihydroartemisinin binding are protein kinases, and 25% are non-protein kinases. Hydroxyl functional group of dihydroartemisinin contributed to 58.5% of the total hydrogen interactions, while pyran (12.2%), endoperoxide (9.8%), and oxepane (19.5%) contributed to the remaining hydrogen bonding. The present findings have elucidated the possible antitumor properties of dihydroartemisinin through the structural-based virtual studies, which provides a lead to a safe and effective anticancer agent useful for cancer therapy.Communicated by Ramaswamy H. Sarma.

Background Pulmonary embolism (PE) is a known cause of morbidity and mortality. A diagnosis of PE is made by computed tomography pulmonary angiogram (CTPA) or a ventilation-perfusion (V/Q) scan. This study aimed to assess the incidence and predictors of acute kidney injury (AKI) in patients with suspected PE. Methods This study was a retrospective study including patients with suspected PE who underwent a CTPA and/or a V/Q scan from 2015 to 2020. The patients were grouped into CTPA or V/Q scan. Creatinine levels were obtained before and after the procedure. AKI was defined based on an increased serum creatinine by 0.3 mg/dL within 48 hours. Results A total of 752 patients were included in the study. The majority (n = 688) underwent a CTPA as a diagnostic modality in patients suspected to have pulmonary embolism (PE), and a V/Q scan was used in 73 patients. Of the 752 patients, there were eight patients who underwent both diagnostic modalities. PE was diagnosed in 121 (16.1%) patients. The incidence of AKI was observed in 15.8%. PE was suspected more frequently in the female group (n = 481, 64%), with a 50% reduction of AKI risk, compared with the male group (p-value = 0.004, OR = 0.522, 95% CI = 0.337-0.81). The presence of diabetes mellitus (DM) and hypertension (HTN) was associated with AKI (p-value < 0.001). Of the AKI group, 43 (36.1%) patients had malignancy. The presence of malignancy was a predictor of increased AKI risk (p-value = 0.014, OR = 1.74, 95% CI = 1.21-2.70). A small proportion (2.1%, n = 16) required dialysis. Patients who developed AKI had a 30-day mortality of 20.2% compared with 5.1% for the group without AKI. Conclusion In our sample, clinicians suspected PE more frequently in the female group. The overall incidence rate of AKI in patients suspected of having PE was 16.1%. The presence of diabetes mellitus and hypertension was associated with AKI. However, DM and HTN were not predictors of AKI. The risk of AKI requiring dialysis was relatively low (2.1%). There was no relationship between the diagnostic modalities and PE, and AKI, suggesting that clinicians overestimate the fear of contrast-induced AKI (CI-AKI)., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2022, Alhassan et al.)

Background  Incisional hernia post organ transplant increases morbidity and impacts quality of life among patients undergoing abdominal organ transplants. Objectives To estimate the incidence rate of incisional hernia and the factors associated with incisional hernia among patients who underwent liver and kidney transplants. Methods This was a retrospective cohort study in which all patients from 2015 to 2020 who underwent liver and/or kidney transplants and met inclusion criteria were involved. Results A total of 424 patients who received transplantation surgery were included. Out of them, 287 patients (67.6%) underwent kidney transplants while 132 patients (31.1%) underwent a liver transplant. Additionally, five patients (1.1%) received both liver and kidney transplantation. Fourteen patients (3.3%) experienced incisional hernia across all samples. A higher incidence rate was noticed among patients with liver transplants compared to kidney transplants (6.81% in the liver group vs 1.7% in the kidney group), which showed a statistical significance between the two groups (P-value= 0.007). In multivariate analysis, surgical site infection (SSI), donor type, acute organ rejection, mycophenolate mofetil (MMF), and diabetes were all not predictors of incisional hernia among the patients.  Conclusion Incisional hernia incidence in between the groups was within the global range of incisional hernia incidence among abdominal organ transplant patients, with a higher incidence among liver transplant patients. All factors associated with incisional hernia, such as SSI, DM, and old age, didn't show significance as predictors to incisional hernia formation among the samples., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2021, Alhassan et al.)

Context: Pseudocedrela kotschyi (Schweinf) Harms (Meliaceae) is an important medicinal plant found in tropical and subtropical countries of Africa. Traditionally, P. kotschyi is used in the treatment of various diseases including diabetes, malaria, abdominal pain and diarrhoea., Objective: To provide an overview of traditional medicinal claims, pharmacological properties, and phytochemical principles of P. kotschyi as a basis for its clinical applications and further research and development of new drugs., Methods: Through interpreting already published scientific manuscripts retrieved from different scientific search engines, namely, Medline, PubMed, EMBASE, Science Direct and Google scholar databases, an up-to-date review on the medicinal potentials of P. kotschyi from inception until September, 2020 was compiled. ' Pseudocedrela kotschyi ', 'traditional uses', 'pharmacological properties' and 'chemical constituents' were used as search words., Results: At present, more than 30 chemical constituents have been isolated and identified from the root and stem bark of P. kotschyi , among which limonoids and triterpenes are the main active constituents. Based on prior research, P. kotschyi has been reported to possess anti-inflammatory, analgesic, antipyretic, anthelminthic, antimalaria, anti-leishmaniasis, anti-trypanosomiasis, hepatoprotective, antioxidant, antidiabetic, antidiarrheal, antimicrobial, and anticancer effects., Conclusions: P. kotschyi is reported to be effective in treating a variety of diseases. Current phytochemical and pharmacological studies mainly focus on antimalaria, anti-leishmaniasis, anti-trypanosomiasis and anticancer potential of the root and stem bark of P. kotschyi . Although experimental data support the beneficial medicinal properties of this plant, there is still a paucity of information on its toxicity profile. Nonetheless, this review provides the basis for future research work.

Introduction The novel coronavirus, officially known as COVID-19, was first reported in Wuhan, China, in December of 2019. Since that time, medical services in Saudi Arabia have adapted to the situation by delivering medical care via virtual clinics. Therefore, the aim of the study is to assess physicians' perception and the level of satisfaction with telemedicine during the COVID-19 pandemic in Riyadh, Saudi Arabia. Methods This was a cross-sectional study that included family medicine consultants and fellows who had used telemedicine in primary health care centers in Riyadh, Saudi Arabia. It was conducted using an online validated questionnaire. The questionnaire was completed by 219 family medicine consultants and fellows, after obtaining their informed consent. The data that were extracted from the questionnaire included demographics, level of satisfaction, and questions related to their experience with telemedicine. Results Two hundred and nineteen participants enrolled in this study with 50.6% males and 49.4% females. The overall level of physicians' satisfaction with telemedicine was 64.3%. However, only one-third preferred telemedicine over office visits. Of these, 60% were males and 40% were females. The only factor that had a statistically significant effect on the preference of office visits or telemedicine was time efficiency (p-value < 0.001). Of those who preferred office visits over telemedicine, 52% of them cited ease of discussion and the ability to make a comprehensive physical examination as the most important reasons for choosing office visits. Technologic issues were the least important factor for choosing either clinic (4.1%). Of those who preferred telemedicine, avoiding contact with patients suspected of COVID-19 was the most commonly cited factor (27.4%). Family medicine physicians face multiple barriers while using telemedicine during the COVID-19 pandemic. The most commonly cited barrier was the inability to make a full and comprehensive assessment of the patient. Conclusions In the setting of highly transmissible disease epidemics, telemedicine has a lot of potential for providing quick and safe care that is appropriate for screening and management. Based on our findings, using telemedicine should be encouraged by improving physicians' skills in this field since telemedicine is a crucial step to reduce the risk of COVID-19 transmission and provide community-wide treatment., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2021, Altulaihi et al.)

Background Globally, less than 10% of graduating medical students select pathology as a future career. Many factors were reported from different settings to influence the choice of pathology. The aim of this study was to investigate the factors that determine medical students' preferences in choosing pathology as a future career. Methods This cross-sectional study surveyed students from three governmental medical schools in Riyadh, Saudi Arabia. A self-administered questionnaire that contained demographic questions and items that addressed perceived factors that affect the choice of pathology was distributed to medical students. Collected data were analyzed using Statistical Product and Service Solutions (SPSS) Statistics for Windows, Version 25.0 (Armonk, NY: IBM Corp). A chi-square test was used to determine the association between independent variables and interest in pathology. Results Out of the 400 questionnaires distributed, 338 students completed the survey with a response rate of 84.5%. Overall, surgery (24%) and internal medicine (20%) were the most favored, specialties with only 5% of the students selecting pathology as their first choice. Patient-doctor interaction (72.2%) was perceived as the most important factor in not choosing pathology as a future career. Taking an elective course, younger age groups, and year in medical school were all significantly associated (p<0.001) with an interest in pathology. Conclusion In this study, most of the students indicated surgery and internal medicine as their first specialty choices. Only 5% of the students chose pathology as their first choice. Two-thirds of medical students perceived pathologists do not interact with patients. A significant association was found between younger age groups, enrolling for a pathology course, and having an interest in pathology., Competing Interests: The authors have declared that no competing interests exist., (Copyright © 2021, Masuadi et al.)

Competing Interests: There are no conflicts of interest.

The effects of climate change such as dry spells, floods and erosion heavily impact agriculture especially smallholder systems on the Northwestern Loess Plateau of China. Nonetheless agriculture also contributes to global warming through the emission of greenhouse gases such as CO 2 , CH 4 and N 2 O. Yet this complex conundrum can be alleviated and mitigated through sound soil and water management practices. Despite considerable literature on Conservation Agriculture (CA) as a strategy to improve the resilience and mitigation capacity of agroecosystems, there is still paucity of information on the impacts of CA on crop production and environmental quality on the Plateau. In order to fill this gap this study examined the effects of no-till and straw mulch on crop productivity and greenhouse gas fluxes in agroecosystems on the Plateau where farmers' common practice of conventional tillage (CT) was tested against three CA practices: conventional tillage with straw mulch (CTS), no-till (NT) and no-till with straw mulch (NTS). The results indicated that all three CA practices (CTS, NT and NTS) markedly increased soil water content (SWC), soil organic carbon (SOC) and soil total nitrogen (STN) but reduced soil temperature (ST). Average grain yields were 854.46 ± 76.51, 699.30 ± 133.52 and 908.18±38.64 kg ha -1 respectively under CTS, NT and NTS indicating an increase by approximately 33%, 9% and 41% respectively compared with CT (644.61 ± 76.98 kg ha -1 ). There were significant ( p  < 0.05) reductions of Net CO 2 emissions under NT (7.37 ± 0.89 tCO2e ha -1 y -1 ) and NTS (6.65 ± 0.73 tCO2e ha -1 y -1 ) compared with CTS (10.65 ± 0.18 tCO2e ha -1 y -1 ) and CT (11.14 ± 0.58 tCO2e ha -1 y -1 ). All the treatments served as sinks of CH 4 but NTS had the highest absorption capacity (-0.27 ± 0.024 tCO2e ha -1 y -1 ) and increased absorption significantly ( p  < 0.05) compared with CT (-0.21 ± 0.017 tCO2e ha -1 y -1 ); however, CA did not reduce emissions of N 2 O. These had an influence on Global warming potential (GWP) as NT and NTS resulted in significant reduction in net GWP. Grain yield was significantly correlated positively with SOC and STN ( p  < 0.05); ecosystem respiration was also significantly correlated with SWC and ST while CH 4 flux was highly correlated with ST ( p  < 0.001). Crop yield and GHG responses to CA were controlled by soil hydrothermal and nutrient changes, thus improving these conditions through adoption of sustainable soil moisture improvement practices such as no-till, straw mulch, green manuring, contour ploughing and terracing can improve crop resilience to climate change and reduce GHG emissions in arid and semi-arid regions., Competing Interests: The authors declare there are no competing interests., (©2021 Alhassan et al.)

Background: The novel coronavirus, officially known as COVID-19, was first reported in Wuhan, China in December of 2019. Since that time, medical services in Saudi Arabia have adapted to the situation by delivering medical care via virtual clinics. Therefore, the present study aimed to assess patients' level of satisfaction with virtual clinics during the COVID-19 pandemic in Saudi Arabia., Materials and Methods: This cross-sectional study was conducted among patients who had experienced virtual clinics in primary healthcare centers in Riyadh, Saudi Arabia. An online validated questionnaire was sent to all participants who had at least one virtual visit between March 2020 to July 2020. The data sought included demographics, level of satisfaction and questions related to their experience with virtual clinics. Computed frequencies and percentages for categorical variables, and median, mean, and standard deviation for continuous variables. Satisfaction scores were compared between groups using Mann-Whitney U test and Kruskal Wallis test., Results: A total of 439 patients completed the questionnaire (response rate 97.5%); 54% were male. The participants were divided into three age groups: 18-39, 40-59, and ≥60 years. Overall level of patients' satisfaction with virtual clinic was 68.1%. Factors statistically significantly associated with satisfaction included gender, age group and level of education (post-graduate and middle school) and being well-informed on the use of telemedicine. Specific age groups that were significant were 18-39 and 40-59 years; 50.2% of the males found telemedicine very convenient, compared to only 36.1% females. Family medicine clinics were the most commonly visited virtual clinics, whereas obstetrics and gynecology clinics were the least attended virtual clinics. The inability to meet the health-care professional face-to-face was reported by 53.8% as the most important disadvantage., Conclusion: This study shows a high level of satisfaction with virtual clinics in Saudi Arabia during the COVID-19 pandemic despite the service being relatively new in healthcare service in the country. Our study demonstrated that satisfaction was linked to age, gender, education and the type of clinic used., Competing Interests: There are no conflicts of interest., (Copyright: © 2021 Journal of Family and Community Medicine.)

In recent years, there is emerging evidence that isoflavonoids, either dietary or obtained from traditional medicinal plants, could play an important role as a supplementary drug in the management of type 2 diabetes mellitus (T2DM) due to their reported pronounced biological effects in relation to multiple metabolic factors associated with diabetes. Hence, in this regard, we have comprehensively reviewed the potential biological effects of isoflavonoids, particularly biochanin A, genistein, daidzein, glycitein, and formononetin on metabolic disorders and long-term complications induced by T2DM in order to understand whether they can be future candidates as a safe antidiabetic agent. Based on in-depth in vitro and in vivo studies evaluations, isoflavonoids have been found to activate gene expression through the stimulation of peroxisome proliferator-activated receptors (PPARs) (α, γ), modulate carbohydrate metabolism, regulate hyperglycemia, induce dyslipidemia, lessen insulin resistance, and modify adipocyte differentiation and tissue metabolism. Moreover, these natural compounds have also been found to attenuate oxidative stress through the oxidative signaling process and inflammatory mechanism. Hence, isoflavonoids have been envisioned to be able to prevent and slow down the progression of long-term diabetes complications including cardiovascular disease, nephropathy, neuropathy, and retinopathy. Further thoroughgoing investigations in human clinical studies are strongly recommended to obtain the optimum and specific dose and regimen required for supplementation with isoflavonoids and derivatives in diabetic patients.

This study was designed to explore and record various medicinal plants integrated into the traditional system of medicine for the treatment of cancer. The traditional system of medicine is a routine practiced among the indigenous ethnic groups of Sokoto state. A semi-structured questionnaire was designed and used for data collection around the selected Local Government Areas. A substantial number of plant species were identified, recorded, and collected for preservation. Data collected for each specie was analysed to assess its frequent use among the medicinal plants. A total of 67 species belonging to 31 families have been identified and recorded. Out of the 473 frequency of citation (FC), Acacia nilotica was the most frequently cited specie (32 FC, 64% FC, 0.6 RFC), followed by Guiera senegalensis (27 FC, 54% FC, 0.5 RFC), Erythrina sigmoidea (17 FC, 34% FC, 0.3 RFC), and subsequently Combretum camporum (15 FC, 30% FC, 0.3 RFC). The most common parts of the plants used include the barks (55.2%), the roots (53.2%), and the leaves (41.8%). Additionally, decoction (74.6%), powdered form (49.3%), and maceration (46.3%) are the most frequently used mode of preparation. The historical knowledge of a traditional system of medicine practiced by the native traditional healers of Sokoto for the treatment of cancer has been documented. The present study further provides a baseline for future pharmacological investigations into the beneficial effects of such medicinal plants for the treatment of cancer., (© 2020 Published by Elsevier Ltd.)

The bioactivity guided fractionation of Tetracera indica leaves crude ethanolic extract has afforded the isolation and characterization of six compounds including a new natural product viz., 5,7-dihydroxyflavone-O-8-sulphate (1) and five known flavonoids (2-6). The structures of the compounds were elucidated using 1D and 2D NMR and HRESIMS spectroscopic analyses. All the isolated compounds were evaluated for their in vitro inhibitory activity against alpha-glucosidase. Compound 1, 5 and 6 showed strong alpha-glucosidase inhibitory activity, 3 and 4 displayed weak activity while compound 2 was inactive. The interactions of the active compounds with alpha-glucosidase were further investigated using molecular docking to confirm their antidiabetic potential.

Flavonoids have been reported to exert antihyperglycemic effects and have potential to enhance the current therapy options against type 2 diabetes mellitus. However, the structure activity relationships (SAR) studies of flavonoids against this disease have not been thoroughly comprehended. Hence, in the present study, 14 structurally related flavonoids viz. wogonin, techtochrysin, norwogonin, isoscutellarein, hypolaetin, kaempferol, quercetin, methyl ether of wogonin, acetate of wogonin, acetate of norwogonin, 8-hydroxy-7-methoxyflavone, chrysin, (+)-catechin and (-)-epicatechin were taken into account for in vitro antidiabetic evaluation. Cell viability of RIN-5F pancreatic cells and 3T3-L1 pre-adipocyte cells was initially tested, then an insulin secretion assay of RIN-5F as well as adipogenesis and glucose uptake measurements of adipocyte were investigated. Subsequently, protein expressions study through adipokines measurement (leptin, adiponectin, TNF-α, RBP-4) via enzyme-linked immunosorbent assay (ELISA) kit, Western blotting analysis against GLUT4 and C/EBP-α as well as molecular docking against GLUT1 were analyzed. The results from cell culture antidiabetic assays (insulin secretion, adipogenesis, and glucose uptake), protein expressions and molecular docking pointed that the methoxy group at position C-8 is responsible for antidiabetic property of selected flavonoids via glucose uptake mechanism indicated by up regulation of GLUT4 and C/EBP-α expressions. The mechanism could be enhanced by the addition of an acetate group at C-5 and C-7 of the flavone skeleton.

The study was conducted during the growing seasons of 2013, 2014, and 2015 in the wet meadows on the eastern Qinghai-Tibet plateau (QTP) in the Gansu Gahai Wetland Nature Reserve to determine the dynamics of soil organic carbon (SOC) as affected by vegetation degradation along a moisture gradient and to assess its relationship with other soil properties and biomass yield. Hence, we measured SOC at depths of 0-10, 10-20, and 20-40 cm under the influence of four categories of vegetation degradation (healthy vegetation [HV], slightly degraded [SD], moderately degraded [MD], and heavily degraded [HD]). Our results showed that SOC decreased with increased degree of vegetation degradation. Average SOC content ranged between 36.18 ± 4.06 g/kg in HD and 69.86 ± 21.78 g/kg in HV. Compared with HV, SOC content reduced by 30.49%, 42.22%, and 48.22% in SD, MD, and HD, respectively. SOC significantly correlated positively with soil water content, aboveground biomass, and belowground biomass, but significantly correlated negatively with soil temperature and bulk density ( p  < 0.05). Highly Significant positive correlations were also found between SOC and total nitrogen ( p  = 0.0036), total phosphorus ( p  = 0.0006) and total potassium ( p  < 0.0001). Our study suggests that severe vegetation and moisture loss led to approximately 50% loss in SOC content in the wet meadows, implying that under climate warming, vegetation and soil moisture loss will dramatically destabilize carbon sink capacities of wetlands. We therefore suggest wetland hydrological management, restoration of vegetation, plant species protection, regulation of grazing activities, and other anthropogenic activities to stabilize carbon sink capacities of wetlands.

The objective of the present study was to investigate the antiradical and xanthine oxidase inhibitory effects of Averrhoa bilimbi leaves. Hence, crude methanolic leaves extract and its resultant fractions, namely hexane, chloroform, and n-butanol were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and xanthine oxidase inhibitory activity. The active constituents were tentatively identified through LC-QTOF-MS/MS and molecular docking approaches. The n-butanol fraction of A. bilimbi crude methanolic leaves extract displayed significant DPPH radical scavenging effect with IC 50 (4.14 ± 0.21 μg/mL) ( p < 0.05), as well as xanthine oxidase inhibitory activity with IC 50 (64.84 ± 3.93 μg/mL) ( p < 0.05). Afzelechin 3- O -alpha-l-rhamnopyranoside and cucumerin A were tentatively identified as possible metabolites that contribute to the antioxidant activity of the n-butanol fraction.

Background: Falls among the elderly are one of the major causes of morbidity and mortality worldwide. They constitute the second leading cause of unintentional deaths after road-traffic accidents. The aim of the study was to estimate the prevalence of falls among the elderly and to investigate the factors that contribute to this phenomenon., Materials and Methods: A cross-sectional analytical study was conducted in the elderly, over the age of 60, in Riyadh. The sample under scrutiny was estimated to be 357 in total. A pilot study was conducted among 15 subjects. Two questionnaires were used for the interviews which were translated into Arabic using the "Morse Fall Scale." The questionnaires assessed: the participants' previous history of falls, whether a secondary diagnosis had been obtained, whether any ambulatory aids had been used or whether an IV connection had been fitted during convalescence. They also inquired if any gait/transferring device had been used to assist the patient at any time., Results: Out of 357 participants, 206 (57.7%) had a history of falls. Study found an association between the number of falls recorded, the age of the participants, and whether the participant was female. Furthermore, there were statistically significant associations between the history of falls and a condition of impaired health. The results also showed that environmental hazards play a significant role in the occurrence of falls with P ≤ 0.001, in which 103 (81.7%) of the individuals who were exposed to environmental hazards revealed a history of falls., Conclusion: Falls among the elderly are common. Significantly, if the health of the individuals is impaired, and there are contiguous environmental risk factors, these elements combine to play a part in the occurrence of such falls. There is, therefore, a need to design and develop a health awareness program to prevent such problems in the elderly., Competing Interests: There are no conflicts of interest.

R273H mutant p53 is a DNA-contact mutant that renders p53 dysfunctional due to a single substitution of Arg273 for His273. Rescuing R273 mutant p53 implies that a competent molecule would have to bind to the site of DNA-contact hot spots to complement the loss of contact with the DNA-binding domain. Here, curcumin, flavokawain B, and alpinetin were docked against the crystal structure of R273H mutant p53 in silico . Consequently, all the compounds bind to the cavity of R273H mutant p53 with a dissociation constant estimated to have 36.57, 70.77, and 75.11 µM for curcumin, flavokawain B, and alpinetin, respectively. Subsequently, each molecule was able to bind to the R273H mutant p53 by interacting with the DNA-contact hot spot Arg248 and mutant R273H, thereby compensating for the loss of direct contact with the DNA-binding domain. Furthermore, all the molecules were able to induce a direct contact with the consensus site of the DNA binding domain, thus maintaining DNA-contact residues with the DNA. The present findings offer preliminary indirect supporting evidence that small molecular weight compounds may certainly rescue DNA-contact mutant p53, which may lay a foundation for designing a competent and effective molecule capable of rescuing mutant p53 in tumor cells expressing R273H mutant p53.

Background: Tetracera indica Merr. (Family: Dilleniaceae), known to the Malay as 'Mempelas paya', is one of the medicinal plants used in the treatment of diabetes in Malaysia. However, no proper scientific study has been carried out to verify the traditional claim of T. indica as an antidiabetic agent. Hence, the aims of the present study were to determine the in vitro antidiabetic potential of the T. indica stems ethanol extract, subfractions and isolated compounds., Methods: The ethanol extract and its subfractions, and isolated compounds from T. indica stems were subjected to cytotoxicity test using MTT viability assay on 3T3-L1 pre-adipocytes. Then, the test groups were subjected to the in vitro antidiabetic investigation using 3T3-L1 pre-adipocytes and differentiated adipocytes to determine the insulin-like and insulin sensitizing activities. Rosiglitazone was used as a standard antidiabetic agent. All compounds were also subjected to fluorescence glucose (2-NBDG) uptake test on differentiated adipocytes. Test solutions were introduced to the cells in different safe concentrations as well as in different adipogenic cocktails, which were modified by the addition of compounds to be investigated and in the presence or absence of insulin. Isolation of bioactive compounds from the most effective subfraction (ethyl acetate) was performed through repeated silica gel and sephadex LH-20 column chromatographies and their structures were elucidated through 1 H-and 13 C-NMR spectroscopy., Results: Four monoflavonoids, namely, wogonin, norwogonin, quercetin and techtochrysin were isolated from the T. indica stems ethanol extract. Wogonin, norwogonin and techtochrysin induced significant (P < 0.05) adipogenesis like insulin and enhanced adipogenesis like rosiglitazone. Wogonin and norwogonin also exhibited significant (P < 0.05) glucose uptake activity., Conclusion: The present study demonstrated that the flavonoids isolated from the T. indica stems possess antidiabetic potential revealing insulin-like and insulin-sensitizing effects which were significant among the compounds. This also rationalizes the traditional use of T. indica in the management of diabetes in Malaysia.

The best described pharmacological property of flavonoids is their capacity to act as potent antioxidant that has been reported to play an important role in the alleviation of diabetes mellitus. Flavonoids biochemical properties are structure dependent; however, they are yet to be thoroughly understood. Hence, the main aim of this work was to investigate the antioxidant and antidiabetic properties of some structurally related flavonoids to identify key positions responsible, their correlation, and the effect of methylation and acetylation on the same properties. Antioxidant potential was evaluated through dot blot, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ABTS + radical scavenging, ferric reducing antioxidant power (FRAP), and xanthine oxidase inhibitory (XOI) assays. Antidiabetic effect was investigated through α -glucosidase and dipeptidyl peptidase-4 (DPP-4) assays. Results showed that the total number and the configuration of hydroxyl groups played an important role in regulating antioxidant and antidiabetic properties in scavenging DPPH radical, ABTS + radical, and FRAP assays and improved both α -glucosidase and DPP-4 activities. Presence of C-2-C-3 double bond and C-4 ketonic group are two essential structural features in the bioactivity of flavonoids especially for antidiabetic property. Methylation and acetylation of hydroxyl groups were found to diminish the in vitro antioxidant and antidiabetic properties of the flavonoids.

Averrhoa bilimbi Linn. is principally cultivated for medicinal purposes in many tropical and subtropical countries of the world. Literature survey about this plant shows that A. bilimbi is mainly used as a folk medicine in the treatment of diabetes mellitus, hypertension, and as an antimicrobial agent. The prime objective of this review is to accumulate and organize literature based on traditional claims and correlate those with current findings on the use of A. bilimbi in the management of different ailments. Through interpreting already published scientific manuscripts (1995 through 2015) retrieved from the different scientific search engines, namely Medline, PubMed, EMBASE, and Science Direct databases, published articles and reports covering traditional and scientific literature related to A. bilimbi 's potential role against various ailments have been thoroughly evaluated, interpreted, and discussed. Several pharmacological studies have demonstrated the ability of this plant to act as antidiabetic, antihypertensive, thrombolytic, antimicrobial, antioxidant, hepatoprotective, and hypolipidemic agent. A. bilimbi holds great value in the complementary and alternative medicine as evidenced by the substantial amount of research on it. Therefore, we aimed to compile an up-to-date and comprehensive review of A. bilimbi that covers its traditional and folk medicine uses, phytochemistry, and pharmacology. Hence, this paper presents an up-to-date and comprehensive review of the ethnomedicinal uses, different chemical constituents, and pharmacological activities of A. bilimbi . So far, the biologically active agents have not been isolated from this plant and this can be a good scientific study for the future antidiabetic, antihypertensive, and antimicrobial implications. Hence, this review targets at emphasizing the diverse traditional claims and pharmacological activities of A. bilimbi with respect to carrying out more scientific studies to isolate active principles through advanced technology., Competing Interests: There are no conflicts of interest.

Maerua angolensis DC is traditionally used for the treatment of epilepsy and insomnia. The present study was designed to investigate the anxiolytic, sedative and toxicological effect of hydromethanolic stem bark extract of M. angolensis using animal model. Sub-chronic doses of the plant extract on liver and kidney function test were investigated. Elevated plus maze (EPM) and diazepam-induced sleeping time test was used in this investigation. The possible involvement of M. angolensis with GABAA receptor was also investigated using flumazenil. The results of acute toxicity studies showed LD50 to be greater than 5000mg/kg body weight. The test extract (40 and 80mg/kg) significantly (p<0.05) increased the number of open arm entries and time spent in the open arm entries. However, flumazenil with 80mg/kg plant extract showed no significant (p >0.01) difference in the number of entries into open arm when compared to control. The stem bark extract of M. angolensis significantly (p<0.01) increased the duration of sleep induced by diazepam in a dose-dependent manner. However, flumazenil with 80mg/kg extract showed no significant (p>0.01) sedative effect when compared to normal control. In conclusion, the result of our present findings revealed that M. angolensis may apparently be safe and non toxic at therapeutic dose. However, the plant may possess anxiolytic and sedative properties, which exert their effect on GABAA receptors.

The biological properties and pathogenicity of seven Newcastle disease virus field isolates were studied. These isolates were recovered from different outbreaks in Sudan (5 from chickens and 2 from pigeons) during 2008-2013. Based on intracerebral pathogenicity index, four NDV isolates were characterized as velogenic (their ICPI ranged 2.0-1.6) and three isolates were characterized as mesogenic (ICPI ranged 1.2-1.3). The mean death time for all isolates ranged from 54 to 76.8 hours. The elution time of the viruses from chicken erythrocytes and the ability to haemagglutinate mammalian red blood cells differed considerably in their reactions.

Aim: To compare different outcomes of vitrification and slow freezing of isolated pre-antral follicles and to evaluate different cryo-devices for vitrification of isolated follicles., Methods: Pre-antral follicles were isolated from mouse ovaries and cryopreserved using vitrification and slow freezing. A preliminary experiment was carried out to select the optimal cryo-device for vitrification of isolated follicles. A total of 414 follicles were randomly distributed among four groups: control (CT) fresh (n=100), nylon mesh (n=96), electron microscopy grid (n=102), and micro-capillary tips (n=116). Subsequently, a total of 979 follicles were randomly assigned to three different groups: CT fresh (n=256), vitrification (n=399) and slow freezing (n=324). CT and cryopreserved/thawed follicles were cultured in vitro and examined daily for development. Final maturation was triggered with human chorionic gonadotrophin and rates of oocyte maturation were calculated. The ultra-structure of cryopreserved/thawed follicles was studied using electron microscopy. Meiotic spindle presence and organization in mature oocytes were examined using the Oosight imaging system., Results:   Micro-capillary tips resulted in poor immediate post-warming survival but no differences were observed in the subsequent in vitro development characteristics between different cryo-devices. Nylon mesh proved to be the easiest carrier, particularly when large numbers of follicles were to be vitrified. Compared to vitrification, slow freezing resulted in a significantly lower number of intact follicles at the end of the culture period (P<0.0001). However all other outcome measures were comparable between both techniques., Conclusions: Isolated follicles were more vulnerable to cryodamage after slow freezing as compared to vitrification., (© 2010 The Authors. Journal of Obstetrics and Gynaecology Research © 2010 Japan Society of Obstetrics and Gynecology.)