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espanolResumen: Se presentan las evidencias arquitectonicas del sitio Cerro Blanco Sur como punto de partida para una caracterizacion de sus patrones formales y, sobretodo, para realizar un analisis de su proceso constructivo. El analisis formal de la arquitectura revela patrones comparables con los conocidos complejos arquitectonicos del valle de Casma, llevandonos a expandir las posibilidades de interpretacion de las dinamicas regionales durante el periodo Formativo Temprano. Por otro lado, el registro de los paramentos de muros de mas de 80 m de largo en la estructura del sitio central ha permitido reconstruir parte del proceso constructivo de dicho edificio, contemplando un escenario de construccion de los muros a partir del levantamiento de pequenos segmentos en forma secuenciada; patron que nos permite contemplar como hipotesis un escenario de pequenas cuadrillas de trabajadores aportando en forma alternada a la construccion final, no siendo necesario el reclutamiento de grandes contingentes de mano de obra. Se trata de un estudio pionero que abre nuevas perspectivas para entender las dinamicas socio-politicas detras de los edificios publicos del periodo Formativo. EnglishAbstract: We describe the architectural evidence of the site Cerro Blanco Sur in order to define its formal patterns and evaluate its construction process. The formal analysis reveals similarities with the well-known early architectural complexes of the Casma Valley thus expanding our perspective on regional dynamics during the Early Formative Period. Also the record of the veneers of 80 meter- long walls allows reconstructing the construction process, figuring a scenario where small segments were built in a sequential mode. Hence, it did not seem to have required large-scale recruitment of labor force, but, rather, small groups of masons who worked in a sequential mode during the construction process. Our analysis provides a pioneer study on Early Formative Period construction processes which opens new perspectives for the understanding of the socio-political dynamics behind the building of public architecture during this period.

5567 Background: Hormonal therapy has limited activity in gynecologic (Gyn) cancer treatment. mTOR inhibitors plus aromatase inhibitors (AI's) improve the response rate and response duration in breast cancer patients. We studied this combination in heavily pre-treated women having estrogen receptor positive (ER+) Gyn cancers. Methods: This phase II study combines everolimus and letrozole for ER+ Gyn cancers with disease progression following primary and salvage chemotherapy. 19 patients participated (Ovary-10, Endometrium-7, and Primary Peritoneal Cancer-2). The mean age was 64, prior lines of therapy ranged from 2-7, and median time from diagnosis to study entry was 67 months (m) (range 10-348m). Results: There were no complete responders, but 7 of 19 (37%) patients treated had clinical benefit, with 1 PR and 6 with stable disease. In responding patients, the earliest time to best response was 2m and the median time to progression was 5m (range 5-40+m). The mean number of treatment cycles was 11. Toxicities: The most common adverse events were: hyperglycemia, rash, stomatitis, fatigue, and anemia. 7 patients required dose reductions, and 2 discontinued study drugs due to pneumonitis. The therapy was generally well tolerated in both women < 65 and > 65 years old. Toxicities were less common with reduced everolimus doses. Conclusions: Recurrent Gyn cancers become refractory to chemotherapy. We tested the combination of everolimus plus letrozole in heavily pretreated patients with ER + recurrent disease. 1 patient had a PR and 6 had stable disease with a range of 5-40+m. This oral regimen was generally well tolerated and allowed time without IV chemotherapy, while providing clinical benefit to patients with refractory ER+ Gyn cancers.

We describe the isolation and characteristics of a novel thermophilic bacterium from soil. The organism is a member of the Anoxybacillus genus based on phylogenetic analysis of the 16S rRNA gene. The 16S rRNA of the organism shares >99% sequence identity with those of two species, Anoxybacillus rupiensis and A. geothermalis. We named this isolate as Anoxybacillus sp. strain UARK-01. UARK-01 grows optimally in the presence of oxygen at 55 °C and pH 8. It grew excellently in the presence of lignin as the sole carbon source. Culture supernatant from UARK-01 grown on lignin was rich in laccase activity. The laccase activity was optimal at 90 °C and pH 9, and there was comparable activity at 80 and 100 °C. The crude laccase decolorized approximately 75% of Congo Red in 7 h under optimal conditions. A single laccase gene was identified from the draft genome sequence of Anoxybacillus sp. UARK-01. The UARK-01 laccase (Anox_Lacc) was cloned and overexpressed in Escherichia coli and was partially purified. The partially purified Anox_Lacc decolorized approximately 1.64+/0.21 nanomoles of Congo Red per microgram protein in 30 min at 90 °C and pH 9. Anox_Lacc is a member of the multicopper polyphenol oxidoreductase laccase family (pfam02578 Cu-oxidase_4) and has novel characteristics. Multiple sequence analysis of Anox_Lacc with six homologs from the family revealed four conserved copper ligands and several new residues that are fully conserved. Anox_Lacc is enriched in leucine, glutamine, and lysine, and it contains fewer alanine, arginine, glycine, and serine residues. Skewed amino acid composition of Anox_Lacc likely contributes to the exceptional thermochemical properties of the laccase activity from UARK-01. Both lignin utilization and production of hyperthermostable alkaline laccase are new findings in the Anoxybacillus genus.