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Aims: We examined the cardiovascular effects of celiac disease (CeD) in a humanized mouse model, with a focus on vascular inflammation, endothelial dysfunction, and oxidative stress. Methods and results: NOD.DQ8 mice genetically predisposed to CeD were subjected to a diet regime and oral gavage to induce the disease (gluten group vs. control). We tested vascular function, confirmed disease indicators, and evaluated inflammation and oxidative stress in various tissues. Plasma proteome profiling was also performed.CeD markers were confirmed in the gluten group, indicating increased blood pressure and impaired vascular relaxation. Pro-inflammatory genes were upregulated in this group, with increased CD11b+ myeloid cell infiltration and oxidative stress parameters observed in aortic and heart tissue. However, heart function remained unaffected. Plasma proteomics suggested the cytokine interleukin-17A (IL-17A) as a link between gut and vascular inflammation. Cardiovascular complications were reversed by adopting a gluten-free diet. Conclusion: Our study sheds light in the heightened cardiovascular risk associated with active CeD, revealing a gut-to-cardiovascular inflammatory axis potentially mediated by immune cell infiltration and IL-17A. These findings augment our understanding of the link between CeD and cardiovascular disease providing clinically relevant insight into the underlying mechanism. Furthermore, our discovery that cardiovascular complications can be reversed by a gluten-free diet underscores a critical role for dietary interventions in mitigating cardiovascular risks associated with CeD.

Sperm have thin structures known as flagella whose motion must be regulated in order to reach the egg for fertilization. Large numbers of sperm are typically needed in this process and some species have sperm that exhibit collective or aggregate motion when swimming in groups. The purpose of this study is to model planar motion of flagella in groups to explore how collective motion may arise in three-dimensional fluid environments. We use the method of regularized Stokeslets and a three-dimensional preferred curvature model to simulate groups of undulating flagella, where flagellar waveforms are modulated via hydrodynamic coupling with other flagella and surfaces. We find that collective motion of free-swimming flagella is an unstable phenomenon in long-term simulations unless there is an external mechanism to keep flagella near each other. However, there is evidence that collective swimming can result in significant gains in velocity and efficiency. With the addition of an ability for sperm to attach and swim together as a group, velocities and efficiencies can be increased even further, which may indicate why some species have evolved mechanisms that enable collective swimming and cooperative behavior in sperm.