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1. Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

Author

Rafik Dey, Harley Mount, Alex W. Ensminger, Greg J. Tyrrell, Linda P. Ward, and Nicholas J. Ashbolt

Subjects
Legionellosis, case report, hot tub, Legionella pneumophila, ameba, coculture, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm the environmental and clinical isolates were of common origin.

Published
2019
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2. Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

Author

Harley O’Connor Mount, Nicholas J. Ashbolt, Linda P Ward, Rafik Dey, Greg J Tyrrell, and Alex W Ensminger

Subjects
Microbiology (medical), Serotype, Canada, Epidemiology, 030231 tropical medicine, lcsh:Medicine, Biology, Legionella pneumophila, Disease Outbreaks, Microbiology, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, case report, Public Health Surveillance, lcsh:RC109-216, 030212 general & internal medicine, bacteria, Amoeba, Phylogeny, Legionellosis, Whole Genome Sequencing, Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada, lcsh:R, Dispatch, Isolation (microbiology), medicine.disease, biology.organism_classification, bacterial infections and mycoses, Coculture Techniques, Infectious Diseases, hot tub, ameba, Legionnaires' disease, Legionnaires' Disease, coculture, Genome, Bacterial, Legionnaires’ disease, Legionella pneumophila serogroup
Abstract

Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm the environmental and clinical isolates were of common origin.

Published
2019

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