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Purpose Dysregulation of the PI3K pathway is one of the most common events in breast cancer. Here we investigate the activity of the PI3K inhibitor MEN1611 at both molecular and phenotypic levels by dissecting and comparing its profile and efficacy in HER2 + breast cancer models with other PI3K inhibitors. Methods Models with different genetic backgrounds were used to investigate the pharmacological profile of MEN1611 against other PI3K inhibitors. In vitro studies evaluated cell viability, PI3K signaling, and cell death upon treatment with MEN1611. In vivo efficacy of the compound was investigated in cell line- and patient-derived xenografts models. Results Consistent with its biochemical selectivity, MEN1611 demonstrated lower cytotoxic activity in a p110δ-driven cellular model when compared to taselisib, and higher cytotoxic activity in the p110β-driven cellular model when compared to alpelisib. Moreover, MEN1611 selectively decreased the p110α protein levels in PIK3CA mutated breast cancer cells in a concentration- and proteasome-dependent manner. In vivo, MEN1611 monotherapy showed significant and durable antitumor activity in several trastuzumab-resistant PIK3CA-mutant HER2 + PDX models. The combination of trastuzumab and MEN1611 significantly improved the efficacy compared to single agent treatment. Conclusions The profile of MEN1611 and its antitumoral activity suggest an improved profile as compared to pan-inhibitors, which are limited by a less than ideal safety profile, and isoform selective molecules, which may potentially promote development of resistance mechanisms. The compelling antitumor activity in combination with trastuzumab in HER2 + trastuzumab-resistant, PIK3CA mutated breast cancer models is at the basis of the ongoing B-Precise clinical trial (NCT03767335).

The 3D reconstruction of upper limb anatomy plays a significant role in many biomedical fields such as ergonomics, motion rehabilitation, and prosthesis design. In the last few years, the technical advancement of consumer-grade depth cameras has supported the development of portable and low-cost optical 3D body scanners for healthcare applications. The real-time scanning of human body parts, however, still represents a complex task due to the non-stationary nature of the scanning target. This issue imposes that the scanning time must be reduced as much as possible to minimize scanning artifacts. In this regard, depth cameras can capture geometrical information at video frame rates, thus guaranteeing fast acquisition times. Furthermore, the simultaneous use of multiple sensors would minimize undercut geometries, which impair the 3D reconstruction’s completeness. In this work, a portable 3D optical scanner has been developed by rigidly assembling three Intel® RealSense™ D415 depth cameras on a lightweight circular frame. The three sensors are mutually calibrated, by using a 3D printed calibration specimen, to simultaneously align acquisitions from the three different camera viewpoints for each scanner pose. The system’s effectiveness has been assessed by acquiring the geometry of both a plaster hand and a human hand and comparing the results with those obtained by a high-end stationary structured light scanner. The developed system represents a low-cost handheld alternative to existing body scanners for collecting and storing 3D anatomical data, which can be used in the design process of bespoke medical devices.

The potential of additive manufacturing to produce optimised and customized polymeric parts is often impaired by poor surface finish, low mechanical properties, and insufficient dimensional accurac...

A single-camera stereo-digital image correlation (stereo-DIC) system to obtain 3D full-field vibration measurements is proposed. The optical setup is composed of two planar mirrors and a single low frame rate camera, thus resulting in a compact and low-cost equipment. The two mirrors are used to create pseudo-stereo images of a target surface on the camera sensor, which are then correlated by using stereo-DIC. The image acquisition process is carried out at low frame rates and the Nyquist-Shannon frequency limitation is overcome by adopting a down-sampling approach under the hypothesis that the vibration signal is characterized by a single known frequency component. The developed pseudo-stereo DIC system allows to obtain 3D full-field vibration measurements in a frequency range up to 4 kHz even with an available frame rate (at full resolution) of 178 fps. The effectiveness of the described approach has been verified by performing vibration measurements on a cantilever plate and a turbine blade.

Recent advances in Additive Manufacturing (AM) technologies have allowed a widespread diffusion of their use in different fields. 3D printing is becoming commonplace for biomedical applications requiring the custom fabrication of prostheses and appliances fitting patient-specific anatomies. In this work, the feasibility of a vat photopolymerization technology, based on Digital Light Processing (DLP), has been investigated for the manufacturing of polymeric orthodontic appliances. A custom DLP 3D printer has been developed by exploiting an off-the-shelf digital projector, with the aim at studying the influence of printing parameters on the surface roughness. The feasibility of using Dental LT Clear resin, a biocompatible photopolymer specifically designed for SLA technology, has been finally verified.

Camera calibration plays a fundamental role for 3D computer vision since it is the first step to recover reliable metric information from 2D images. The calibration of a stereo-vision system is a two-step process: firstly, the calibration of the individual cameras must be carried out, then the two individual calibrations are combined to retrieve the relative placement between the two cameras, and to refine intrinsic and extrinsic parameters. The most commonly adopted calibration methodology uses multiple images of a physical checkerboard pattern. However, the process is time-consuming since the operator must move the calibration target into different positions, typically from 15 to 20. Moreover, the calibration of different optical setups requires the use of calibration boards, which differ for size and number of target points depending on the desired working volume. This paper proposes an innovative approach to the calibration, which is based on the use of a conventional computer screen to actively display the calibration checkerboard. The potential non-planarity of the screen is compensated by an iterative approach, which also estimate the actual screen shape during the calibration process. The use of an active display greatly enhances the flexibility of the stereo-camera calibration process since the same device can be used to calibrate different optical setups by simply varying number and size of the displayed squared patterns.

3D digital models of the upper limb anatomy represent the starting point for the design process of bespoke devices, such as orthoses and prostheses, which can be modeled on the actual patient’s anatomy by using CAD (Computer Aided Design) tools. The ongoing research on optical scanning methodologies has allowed the development of technologies that allow the surface reconstruction of the upper limb anatomy through procedures characterized by minimum discomfort for the patient. However, the 3D optical scanning of upper limbs is a complex task that requires solving problematic aspects, such as the difficulty of keeping the hand in a stable position and the presence of artefacts due to involuntary movements. Scientific literature, indeed, investigated different approaches in this regard by either integrating commercial devices, to create customized sensor architectures, or by developing innovative 3D acquisition techniques. The present work is aimed at presenting an overview of the state of the art of optical technologies and sensor architectures for the surface acquisition of upper limb anatomies. The review analyzes the working principles at the basis of existing devices and proposes a categorization of the approaches based on handling, pre/post-processing effort, and potentialities in real-time scanning. An in-depth analysis of strengths and weaknesses of the approaches proposed by the research community is also provided to give valuable support in selecting the most appropriate solution for the specific application to be addressed.

In recent years, Fused Deposition Modelling (FDM) has become one of the most attractive Additive Manufacturing (AM) techniques, due to the advantages in the production of complex shapes with a wide range of materials and low investment costs. The thermoplastic polymers used for FDM technology are characterized by low mechanical properties if compared to those of composites and metals. This issue is usually overcome by reinforcing the thermoplastic polymer with chopped fibres or particles. Moreover, a second issue arises, which is represented by the water absorption with a relevant impact on mechanical properties and dimensional stability of printed models. In this paper, an experimental study is presented with the aim at evaluating the water absorption influence on mechanical properties of Carbon PA (Polyamide matrix reinforced with Carbon Fiber at 20%) specimens fabricated with the FDM technique. Two post-processing treatments, based on the use of acrylic spray and photosensitive resin, have been also proposed to improve the behaviour of Carbon PA printed parts. Results of water absorption tests and tensile tests demonstrated a significant improvement in terms of weight stability and mechanical properties by adopting the proposed post-processing treatments.

Dynamic characterization of vibrating targets represents a critical issue for many industrial fields. In this paper, a stereo-camera system integrated with a Digital Image Correlation (DIC) algorithm is proposed with the aim at performing 3D full-field vibration measurements in the range of kHz. The system exploits two industrial low-speed cameras, and the Nyquist-Shannon frequency limitation is overcome by a down-sampling approach under the hypothesis that the vibration signal is characterized by a single known frequency component. Experimental results obtained from the measurement of vibrational responses of a cantilever plate excited at three high-frequency resonance values (1121 Hz, 2956 Hz and 4010 Hz) are provided. A comparison with numerical analyses evidences the effectiveness of the proposed approach.

Moisture absorption degrades the mechanical properties of polymeric parts that are 3D-printed by fused filament fabrication (FFF). This limitation is particularly significant for short fiber-reinforced polymers because the mechanical enhancement obtained by the fiber reinforcement can be compromised by the plasticizing effect introduced by water absorption. Therefore, the present work investigates the effects of two different coatings, a UV cured acrylate resin and an acrylic varnish, on the moisture absorption of FFF 3D-printed samples consisting of polyamide reinforced by short carbon fibers. Water content (CI) and open porosity (OP) were estimated through water absorption tests in distilled water for 2, 24, and 168 h, and after reconditioning. The coating effects were evaluated by conducting tensile tests to compare the Young’s modulus, yield stress, and ultimate stress of the coated and uncoated specimens. The results demonstrated a significant reduction of CI and OP with both the acrylic and UV resin coatings, as well as considerable enhancements of these samples’ mechanical properties. Stress-strain curves evidenced a strain reduction after water immersion, which can be ascribed to a greater stability against different moisture conditions. These findings indicate the significant potential of the proposed coating processes to extend the use of FFF 3D-printed composite materials to a broader range of applications.

The digital image correlation (DIC) was used in this paper to obtain full-field measurements of a target vibrating at a frequency higher than the maximum cameras’ frame rate. The down-sampling technique was implemented to compensate for the cameras’ moderate frame rate, thus getting an accurate displacement acquisition even at 6.5 kHz. Two innovative methods to support the DIC application were introduced. The use of fringe projection (or structured light), initially applied on the sample at rest, reduced the effort and time required for the stereo matching task’s solution and improved this setting’s accuracy and reliability. Additionally, a new time-domain image filtering was proposed and tested to improve the quality of the obtained DIC maps. In combination with the down-sampling, the effect of this filtering technique was tested in this work at (approx.) 2500 and 6500 Hz by measuring the response of a bladed disk to sinusoidal excitation. Evidence of improved results was observed for both frequencies for amplitudes in the range of 10 µm.

The present paper describes the development and characterization of a structured light stereo catadioptric scanner for the omnidirectional reconstruction of internal surfaces. The proposed approach integrates two digital cameras, a multimedia projector and a spherical mirror, which is used to project the structured light patterns generated by the light emitter and, at the same time, to reflect into the cameras the modulated fringe patterns diffused from the target surface. The adopted optical setup defines a non-central catadioptric system, thus relaxing any geometrical constraint in the relative placement between optical devices. An analytical solution for the reflection on a spherical surface is proposed with the aim at modelling forward and backward projection tasks for a non-central catadioptric setup. The feasibility of the proposed active catadioptric scanner has been verified by reconstructing various target surfaces. Results demonstrated a great influence of the target surface distance from the mirror's centre on the measurement accuracy. The adopted optical configuration allows the definition of a metrological 3D scanner for surfaces disposed within 120 mm from the mirror centre.

In the computer vision field, the reconstruction of target surfaces is usually achieved by using 3D optical scanners assembled integrating digital cameras and light emitters. However, these solutions are limited by the low field of view, which requires multiple acquisition from different views to reconstruct complex free-form geometries. The combination of mirrors and lenses (catadioptric systems) can be adopted to overcome this issue. In this work, a stereo catadioptric optical scanner has been developed by assembling two digital cameras, a spherical mirror and a multimedia white light projector. The adopted configuration defines a non-single viewpoint system, thus a non-central catadioptric camera model has been developed. An analytical solution to compute the projection of a scene point onto the image plane (forward projection) and vice-versa (backward projection) is presented. The proposed optical setup allows omnidirectional stereo vision thus allowing the reconstruction of target surfaces with a single acquisition. Preliminary results, obtained measuring a hollow specimen, demonstrated the effectiveness of the described approach.

BACKGROUND. SEL24/MEN1703 is a first-in-class, orally available, dual PIM/FLT3 kinase inhibitor investigated in unselected AML patients in the First-in-Human, Dose Escalation (DE) and Cohort Expansion (CE) CLI24-001 trial (DIAMOND-01, ClinicalTrials.gov identifier: NCT03008187). The study has completed both the DE and CE part showing an acceptable safety profile up to the recommended dose (RD), with initial evidence of single agent efficacy particularly clustering in patients with IDH mutant disease. Previous studies conducted on a limited number of patients in the DE cohort indicated pS6, a downstream target in the PIM/FLT3 signaling pathway, as a biomarker of target engagement. AIM. To assess the relevance of pS6 as a pharmacodynamic (PD) biomarker in SEL24/MEN1703-treated patients. Moreover, patients were characterized on the basis of genetic profile to identify mutations which might cluster in patients' samples that demonstrated significant target engagement. Finally, longitudinal assessment of Variant Allelic Frequency (VAF) has been implemented. METHODS. S6 phosphorylation has been longitudinally monitored in the DIAMOND-01 study through an optimized assay for multiparametric analysis of phospho-protein activation. The assay allows a quantitative assessment of pS6 at the single cell level among blast cells as well as the identification of blast subpopulations in peripheral blood (PB). CD34+ Blast cells % was monitored to assess whether target engagement translated into blast reduction. Genotype analysis was performed by NGS using the Oncomine Myeloid Research Assay. RESULTS. PB samples from DE and CE patients, treated at 125 mg (RD) were analyzed, for a total of n= 27 assessable patients. At screening, we observed a heterogeneous positivity for pS6 marker in blast cells (range: 0.9%-79%), consistent with the unselected AML patient population recruited in the trial. Overall, 14/27 (51.8%) patients showed pS6 inhibition in blast cells at the end of Cycle 1 in comparison with screening (range: 70%-94%) (Figure 1). When SEL24/MEN1703 treatment continued beyond Cycle 1 (n=11 patients), pS6 inhibition was maintained and long-lasting in 6/11 (54.5%) patients. While pS6 inhibition did not translate into CD34+ blast decrease in all patients, long-lasting pS6 inhibition was observed in 2 out of 3 responding patients (including CRi as best response) evaluable for pS6 levels. Genetic characterization of these two patients treated at 125 mg (RD) showed that they harbored IDH mutations. CONCLUSIONS. The longitudinal PD assessment through the modulation of pS6 activation by flow cytometry confirmed that meaningful target engagement was achieved in more than 50% of patients treated with SEL24/MEN1703 at the RD. In most patients, including 2 out of 3 responders evaluable for pS6, its inhibition was maintained during the entire treatment. Preliminary genotyping showed that responding patients with strong pS6 inhibition harbored IDH mutations. In-depth analysis of genomic profiling and of the longitudinal assessment of VAF is ongoing in order to identify potential correlation with pS6 modulation and blast depletion in specific AML subsets, with the aim to define patient population(s) more likely to respond to SEL24/MEN1703. Figure 1. pS6 (%pS6*MFI) assessment in peripheral blast cells at screening and at C1D14-4H in CE patients treated at RD of 125 mg. Figure 1 Figure 1. Disclosures Paoli: Menarini Group: Current Employment. Bellarosa: Menarini Group: Current Employment. Bressan: Menarini Group: Current Employment. Bertolini: Gilead: Research Funding; Emercell: Research Funding. Montesinos: Teva: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Sanofi: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Stemline/Menarini: Consultancy; Celgene: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Novartis: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Pfizer: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Forma Therapeutics: Consultancy; Janssen: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Karyopharm: Membership on an entity's Board of Directors or advisory committees, Research Funding; Daiichi Sankyo: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Incyte: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Glycomimetics: Consultancy; Tolero Pharmaceutical: Consultancy; Agios: Consultancy; AbbVie: Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; Astellas Pharma, Inc.: Consultancy, Honoraria, Other: Advisory board, Research Funding, Speakers Bureau. Mukherjee: Eusa Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Teaching and Speaking; McGraw Hill: Honoraria, Other: Editor of Hematology Oncology Board Review (ongoing); Celgene Corporation: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees, Other: Research/Independent Contractor, Research Funding; AAMDS in Joint Partnership with Cleveland Clinic Taussig Cancer Institute: Honoraria; Genentech: Membership on an entity's Board of Directors or advisory committees; AbbVie: Membership on an entity's Board of Directors or advisory committees; Jazz Pharmaceuticals: Research Funding; BioPharm: Consultancy; Novartis: Consultancy, Membership on an entity's Board of Directors or advisory committees, Other: Research/Independent Contractor, Research Funding; Bristol-Myers Squibb Co.: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees; Acceleron: Membership on an entity's Board of Directors or advisory committees; Blueprint Medicines: Membership on an entity's Board of Directors or advisory committees; Partnership for Health Analytic Research: Honoraria. Strickland: AbbVie, ArcherDx, Genentech, Incyte, Kura Oncology, Novartis, Pfizer, and Syros: Consultancy; Sunesis: Research Funding. Santoro: Takeda: Speakers Bureau; BMS: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Sanofi: Consultancy; Arqule: Consultancy, Speakers Bureau; Roche: Speakers Bureau; AbbVie: Speakers Bureau; Eli-Lilly: Speakers Bureau; Pfizer: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; AstraZeneca: Speakers Bureau; Sandoz: Speakers Bureau; Eisai: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Gilead: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Servier: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Celgene: Speakers Bureau; Amgen: Speakers Bureau; Novartis: Speakers Bureau; Bayer: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; MSD: Membership on an entity's Board of Directors or advisory committees, Speakers Bureau. Walter: Jazz: Research Funding; Pfizer: Consultancy, Research Funding; Selvita: Research Funding; Amphivena: Consultancy, Other: ownership interests; Agios: Consultancy; Astellas: Consultancy; BMS: Consultancy; Genentech: Consultancy; Janssen: Consultancy; Kite: Consultancy; Macrogenics: Consultancy, Research Funding; Immunogen: Research Funding; Celgene: Consultancy, Research Funding; Aptevo: Consultancy, Research Funding; Amgen: Research Funding. Fazal: Taiho: Consultancy, Honoraria, Speakers Bureau; Stemline: Consultancy, Honoraria, Speakers Bureau; Sanofi Genzyme: Consultancy, Honoraria, Speakers Bureau; Novartis: Consultancy, Honoraria, Speakers Bureau; Karyopharm: Consultancy, Honoraria, Speakers Bureau; Jazz Pharma: Consultancy, Honoraria, Speakers Bureau; Janssen: Consultancy, Honoraria, Speakers Bureau; Incyte: Consultancy, Honoraria, Speakers Bureau; GlaxoSmithKline: Consultancy, Honoraria, Speakers Bureau; Gilead: Consultancy, Honoraria, Speakers Bureau; Celgene: Consultancy, Honoraria, Speakers Bureau; BMS: Consultancy, Honoraria, Speakers Bureau; Amgen: Consultancy, Honoraria, Speakers Bureau; Agios: Consultancy, Honoraria, Speakers Bureau; Takeda: Consultancy, Honoraria, Speakers Bureau; Tolero: Consultancy. Ravandi: Novartis: Honoraria; AstraZeneca: Honoraria; Xencor: Honoraria, Research Funding; Astex: Honoraria, Research Funding; Jazz: Honoraria, Research Funding; Taiho: Honoraria, Research Funding; Prelude: Research Funding; Agios: Honoraria, Research Funding; AbbVie: Honoraria, Research Funding; Amgen: Honoraria, Research Funding; Celgene: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Bristol Myers Squibb: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding; Syros Pharmaceuticals: Consultancy, Honoraria, Research Funding. Brzozka: Ardigen: Current Employment, Membership on an entity's Board of Directors or advisory committees; Ryvu Therapeutics: Current Employment, Current equity holder in publicly-traded company, Membership on an entity's Board of Directors or advisory committees; Selvita SA: Current Employment, Current equity holder in publicly-traded company, Membership on an entity's Board of Directors or advisory committees. Rzymski: Ryvu Therapeutics: Current Employment, Current equity holder in publicly-traded company. Mazan: Ryvu Therapeutics: Current Employment; Selvita SA: Current Employment. Baldini: Menarini Group: Current Employment. Ravenni: Menarini Group: Current Employment. Binaschi: Menarini Group: Current Employment. Laurent: Menarini Group: Current Employment. Pellacani: Menarini Group: Current Employment.

Orthodontic treatments are usually performed using fixed brackets or removable oral appliances, which are traditionally made from alginate impressions and wax registrations. Among removable devices, eruption guidance appliances are used for early orthodontic treatments in order to intercept and prevent malocclusion problems. Commercially available eruption guidance appliances, however, are symmetric devices produced using a few standard sizes. For this reason, they are not able to meet all the specific patient’s needs since the actual dental anatomies present various geometries and asymmetric conditions. In this article, a computer-aided design-based methodology for the design and manufacturing of a patient-specific eruption guidance appliances is presented. The proposed approach is based on the digitalization of several steps of the overall process: from the digital reconstruction of patients’ anatomies to the manufacturing of customized appliances. A finite element model has been developed to evaluate the temporomandibular joint disks stress level caused by using symmetric eruption guidance appliances with different teeth misalignment conditions. The developed model can then be used to guide the design of a patient-specific appliance with the aim at reducing the patient discomfort. At this purpose, two different customization levels are proposed in order to face both arches and single tooth misalignment issues. A low-cost manufacturing process, based on an additive manufacturing technique, is finally presented and discussed.

In the world of powerboats competition, the high-performance sandwich-structured composites have completely replaced traditional materials. During the competition, the structure of this kind of shi...

Background: : personality features have been repeatedly associated with depression treatment outcome in Major Depressive Disorder (MDD), however conclusive results are still lacking. Moreover, as for Bipolar Disorder (BD), results are only few and preliminary. Aim: : the aim of the present study was to perform an exploratory investigation of the influence of personality traits as assessed by the Temperament and Character Inventory (TCI), on principal depression treatment outcomes (non remission, non response and resistance). Methods: : 743 mood disorders patients (455 MDD (61.24%) and 288 BD (38.76%)) were recruited in the context of 6 European studies. Generalized logit models were performed to test the effects of TCI dimensions on treatment outcomes, considering possible confounders such as age, gender and education. Positive results were controlled for comorbidities (anxiety and substance use disorders) as well. Results: : MDD Non-Remitters showed high Harm Avoidance (HA) and Self Transcendence (ST) (p = 0.0004, d = 0.40; p = 0.007, d = 0.36 respectively) and low Persistence (P) and Self Directedness (SD) (p = 0.05; d = 0.18; p = 0.002, d = 0.40, respectively); MDD Non-Responders showed a slightly different profile with high HA and low Reward Dependence (RD) and SD; finally, MDD Resistants showed low RD, P and Cooperativeness (C). In BD patients, only higher HA in non response was observed. Limitations: : the retrospective cross-sectional design, the TCI assessment regardless of the mood state and the small number of bipolar patients represent the main limitations. Conclusion: : specific TCI personality traits are associated with depression treatment outcome in MDD patients. The inclusion of such personality traits, together with other socio-demographic and clinical predictors, could ameliorate the accuracy of the prediction models available to date.

The use of light encoding techniques is widespread in the field of 3D surface reconstruction. This paper presents a stereo-camera calibration methodology, which integrates structured light encoding with an active digital device. The structured light encoding approach is proposed to unambiguously solve the stereo matching issue for stereo-camera setups. A sequence of vertical and horizontal binary striped patterns, combined with a checkerboard pattern, is displayed by a high-resolution LCD screen, which is used as calibration board. A bundle adjustment technique is adopted to simultaneously adjust both camera parameters and screen geometry, as part of the stereo-camera calibration process, thus taking into account the possible inaccuracies of the digital display. The same structured light approach, with small variants, is projected by a multimedia digital projector to carry out 3D surface reconstruction. The proposed methodology defines a comprehensive framework for the development of a 3D optical scanner, from calibration to 3D acquisition, which has been validated by measuring primitive surfaces and reconstructing free-form shapes with different stereo-camera setups.

A 3D full-field optical system for high frequency vibration measurement is proposed. The system is composed of a single low-frame-rate camera and two planar mirrors. This compact optical setup overcomes the typical drawback of capturing synchronous acquisitions in the case of a camera pair. Moreover, planar mirrors allow for the use of the classical pinhole model and, thus, conventional stereo-calibration techniques. The use of a low-frame-rate camera provides on the one hand a high-resolution sensor with a relatively low-cost hardware but imposes, on the other, the adoption of a down-sampling approach, which is applicable only when a single (known) sinusoidal load is applied to the structure. The effectiveness of the proposed setup has been verified by the 3D vibration measurement of two different targets up to a frequency of 1 kHz, corresponding to a displacement amplitude of 0.01 mm.