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1. National, longitudinal NASCITA birth cohort study: prevalence of overweight at 12 months of age in children born healthy

Author

Antonio Clavenna, Massimo Cartabia, Maurizio Bonati, Federico Marchetti, Giorgio Tamburlini, Antonio Addis, Michele Valente, Rita Campi, Stefania Manetti, Valeria Carraro, Sergio Conti Nibali, Renato Sansone, Patrizia Rogari, Annalisa Campomori, Luca De Fiore, Renata Bortolus, Sergio Cattani, Cristiana Piloni, Valeria Renzetti, Rosario Cavallo, Gherardo Rapisardi, Giacomo Toffol, Vicenza Briscioli, Carla Cafaro, Cristoforo Cocchiara, Isodiana Crupi, Patrizia Del Balzo, Laura Dell’edera, Chiara Di Francesco, Alberto Ferrando, Francesca Grassa, Chiara Guidoni, Claudio Mangialavori, Stefano Marinoni, Francesca Marongiu, Fausta Matera, Paolo Moretti, Laura Olimpi, Angela Pasinato, Ilaria Porro, Ippolita Roncoroni, Raffaella Schiro’, Patrizia Seppia, Federica Zanetto, Anna Aloisio, Elisabetta Anedda, Giuliana Apuzzo, Giovanna Argo, Anna Armenio, Emanuela Ballerini, Monica Benedetti, Daniela Bertoli, Stefano Bollettini, Chiara Bottalico, Aurora Bottiglieri, Vincenza Briscioli, Antonella Bruno, Laura Brusadin, Mariantonietta Caiazzo, Patrizia Calamita, Miriana Callegari, Rosaria Cambria, Maria Cristina Cantù, Domenico Capomolla, Anna Caracciolo, Maria Concetta Carbone, Gaetano Carrassi, Maria Laura Cartiglia, Sara Casagranda, Ornella Castiglione, Rosario Salvatore Cavallo, Teresa Cazzato, Maria Angela Cazzuffi, Jennifer Chiarolanza, Rosaria China, Nicoletta Cimadamore, Roberto Cionini, Cristina Ciuffo, Damiano Colazzo, Anna Maria Costantini, Claudio Cravidi, Marialuisa Criscione, Rita D’Agostino, Daniela Danieli, Luigi De Carlo, Marina De Sanctis, Giuseppina De Santes, Gian Piero Del Bono, Maria Elisabetta Di Pietro, Maria Chiara Dini, Paolo Fiammengo, Micaela Foco, Maria Teresa Fonte, Maria Frigeri, Andrea Galvagno, Matteo Gaudino, Stefania Genoni, Silvia Girotto, Gianluca Gornati, Marta Gozzi, Enrica Heritier, Antonella Lavagetto, Raffaele Limauro, Alessandra Magnelli, Maria Gabriella Maiolino, Monica Malventano, Silvia Marchi, Natale Maresca, Federico Marolla, Agata Martinelli, Chiara Martinez, Nicoletta Mascarello, Carla Matiotti, Elisabetta Mazzucchi, Donatella Moggia, Manuela Musetti, Paolo Nardini, Alberto Neri, Patrizia Neri, Flavia Nicoloso, Laura Maria Olimpi, Giancarlo Ottonello, Giacinta Padula, Paolo Maria Paganuzzi, Rosanna Palazzi, Alessandra Palmero, Maria Chiara Parisini, Giovannina Pastorelli, Marilena Pavoni, Lucia Peccarisi, Antonella Pellacani, Cristina Perrera, Michela Picciotti, Ivo Picotto, Tiziana Piunti, Francesca Preziosi, Giuseppe Primavera, Miriam Prodi, Maria Letizia Rabbone, Innocenza Rafele, Laura Reali, Franziska Stefanie Rempp, Ada Riundi, Paolo Rosas, Annarita Russo, Mariagrazia Saccà, Elisabetta Sala, Francesca Sala, Francesca Santus, Vittoria Sarno, Alessandra Savino, Raffaella Schirò, Giuseppa Scornavacca, Maria Francesca Siracusano, Adelisa Spalla, Gloria Sturaro, Maria Grazia Toma, Ettore Tomagra, Maria Tortorella, Fausta Trentadue, Marina Trevisan, Silvia Tulisso, Roberta Usella, Anna Valente, Mariangela Valera, Edda Vernile, Valeria Vicario, Lucia Vignutelli, Paolo Vinci, Lucia Vizziello, Rosette Zand, Marco Zanette, Graziano Zucchi, Maria Luisa Zuccolo, Eleonora Morabito, Chiara Liz Pandolfini, Chiara Segre’, Rossella Claudia Cannavo’, Melania Maria Cera, Emma Maria Giugnini, and Giovanni Giuliano Semprini

Subjects
Pediatrics, RJ1-570
Abstract

Objective To estimate the prevalence of overweight at 12 months in an Italian birth cohort and to identify factors related to an increased likelihood of being overweight.Methods The Italian NASCITA birth cohort was analysed. Infants were classified as underweight (

Published
2023
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2. Postpartum depression screening in mothers and fathers at well-child visits: a feasibility study within the NASCITA cohort

Author

Antonio Clavenna, Massimo Cartabia, Maurizio Bonati, Federico Marchetti, Giorgio Tamburlini, Antonio Addis, Michele Valente, Stefania Manetti, Valeria Carraro, Renato Sansone, Patrizia Rogari, Annalisa Campomori, Renata Bortolus, Sergio Cattani, Cristiana Piloni, Valeria Renzetti, Chiara Segré, Rosario Cavallo, Gherardo Rapisardi, Giacomo Toffol, Vicenza Briscioli, Carla Cafaro, Cristoforo Cocchiara, Isodiana Crupi, Laura Dell’edera, Alberto Ferrando, Francesca Grassa, Chiara Guidoni, Claudio Mangialavori, Stefano Marinoni, Francesca Marongiu, Fausta Matera, Paolo Moretti, Laura Olimpi, Angela Pasinato, Ilaria Porro, Ippolita Roncoroni, Raffaella Schiro’, Patrizia Seppia, Federica Zanetto, Anna Aloisio, Elisabetta Anedda, Giuliana Apuzzo, Giovanna Argo, Anna Armenio, Emanuela Ballerini, Monica Benedetti, Daniela Bertoli, Stefano Bollettini, Chiara Bottalico, Aurora Bottiglieri, Vincenza Briscioli, Antonella Bruno, Laura Brusadin, Mariantonietta Caiazzo, Patrizia Calamita, Miriana Callegari, Rosaria Cambria, Domenico Capomolla, Anna Caracciolo, Gaetano Carrassi, Sara Casagranda, Ornella Castiglione, Teresa Cazzato, Jennifer Chiarolanza, Rosaria China, Nicoletta Cimadamore, Roberto Cionini, Cristina Ciuffo, Damiano Colazzo, Claudio Cravidi, Marialuisa Criscione, Rita D’Agostino, Daniela Danieli, Paolo Fiammengo, Micaela Foco, Maria Frigeri, Andrea Galvagno, Matteo Gaudino, Stefania Genoni, Silvia Girotto, Gianluca Gornati, Marta Gozzi, Enrica Heritier, Antonella Lavagetto, Raffaele Limauro, Alessandra Magnelli, Monica Malventano, Silvia Marchi, Natale Maresca, Federico Marolla, Agata Martinelli, Chiara Martinez, Nicoletta Mascarello, Carla Matiotti, Elisabetta Mazzucchi, Donatella Moggia, Manuela Musetti, Paolo Nardini, Alberto Neri, Patrizia Neri, Flavia Nicoloso, Giancarlo Ottonello, Giacinta Padula, Rosanna Palazzi, Alessandra Palmero, Giovannina Pastorelli, Marilena Pavoni, Lucia Peccarisi, Antonella Pellacani, Cristina Perrera, Michela Picciotti, Ivo Picotto, Tiziana Piunti, Francesca Preziosi, Giuseppe Primavera, Miriam Prodi, Maria Letizia Rabbone, Innocenza Rafele, Laura Reali, Ada Riundi, Paolo Rosas, Annarita Russo, Mariagrazia Saccà, Elisabetta Sala, Francesca Sala, Francesca Santus, Vittoria Sarno, Alessandra Savino, Raffaella Schirò, Giuseppa Scornavacca, Giovanni GiulianoSemprini, Adelisa Spalla, Gloria Sturaro, Ettore Tomagra, Maria Tortorella, Fausta Trentadue, Marina Trevisan, Silvia Tulisso, Roberta Usella, Anna Valente, Mariangela Valera, Edda Vernile, Valeria Vicario, Lucia Vignutelli, Paolo Vinci, Lucia Vizziello, Rosette Zand, Marco Zanette, Graziano Zucchi, Giulia Segre, Luca DeFiore, Sergio ContiNibali, Patrizia DelBalzo, Chiara DiFrancesco, Rossella ClaudiaCannavò, Maria CristinaCantù, Maria ConcettaCarbone, Maria LauraCartiglia, Rosario SalvatoreCavallo, Maria AngelaCazzuffi, MelaniaMaria GiuseppinaCera, Anna MariaCostantini, Luigi DeCarlo, Marina DeSanctis, Giuseppina DeSantes, GianPiero DelBono, MariaElisabetta DiPietro, Maria ChiaraDini, Maria TeresaFonte, EmmaMaria LetiziaGiugnini, Maria GabriellaMaiolino, Laura MariaOlimpi, Paolo MariaPaganuzzi, Maria ChiaraParisini, Franziska StefanieRempp, Maria FrancescaSiracusano, Maria GraziaToma, and Maria LuisaZuccolo

Subjects
Medicine
Abstract

Objective To assess the feasibility of the family paediatrician’s (FP) role in identifying the signs of postpartum depression in parents in time to guarantee child well-being.Design, setting and participants Data for this observational prospective study were collected within the NASCITA (NAscere e creSCere in ITAlia) cohort. During the first visit, paediatricians collected sociodemographic data regarding the parents and information about their health status, the pregnancy and the delivery. Whooley questions were administered during the first and second visits (scheduled 60–90 days after childbirth). Moreover, on the third visit (5–7 months after childbirth) the FP was asked to answer ‘yes’ or ‘no’ to a question on the parental postpartum depression, based on his knowledge and on the acquired information.Results In 2203 couples who completed the assessment, 529 mothers (19.9%), 141 fathers (6.3%) and 110 (5%) couples reported any depressive symptomatology. Of these, 141 mothers (5.3% of the total sample) and 18 fathers (0.8% of the total sample) were classified as ‘likely depressed’. An association was found between maternal postnatal depressive symptoms and having a diagnosed psychiatric disorder during pregnancy (OR 9.49, 95% CI: 3.20 to 28.17), not exclusively breastfeeding at hospital discharge (OR 1.76, 95% CI: 1.19 to 2.61) and the presence of child sleeping disorders at 3 (OR 2.46, 95% CI: 1.41 to 4.28) and 6 months (OR 2.18, 95% CI: 1.37 to 3.47). Another significant predictor of postpartum depression was being primiparous (OR 1.99, 95% CI: 1.31 to 3.02). Concerning the fathers, a significant association was reported only between likely depressed fathers and child sleeping disorders at 3 months (OR 7.64, 95% CI: 2.92 to 19.97). Moreover, having a likely depressed partner was strongly associated with depressive symptoms in fathers (OR 85.53, 95% CI 26.83 to 272.69).Conclusions The findings of this study support the feasibility of an active screening programme for parental postnatal depression during well-child visits as an integral part of postpartum care.Trial registration number NCT03894566; Pre-results.

Published
2023
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3. National, longitudinal NASCITA birth cohort study to investigate the health of Italian children and potential influencing factors

Author

Antonio Clavenna, Massimo Cartabia, Maurizio Bonati, Federico Marchetti, Giorgio Tamburlini, Antonio Addis, Michele Valente, Rita Campi, Stefania Manetti, Valeria Carraro, Chiara Pandolfini, Sergio Conti Nibali, Renato Sansone, Patrizia Rogari, Annalisa Campomori, Luca De Fiore, Renata Bortolus, Sergio Cattani, Cristiana Piloni, Valeria Renzetti, Chiara Segré, Rosario Cavallo, Gherardo Rapisardi, Giacomo Toffol, Vicenza Briscioli, Carla Cafaro, Cristoforo Cocchiara, Isodiana Crupi, Patrizia Del Balzo, Laura Dell’edera, Chiara Di Francesco, Alberto Ferrando, Francesca Grassa, Chiara Guidoni, Claudio Mangialavori, Stefano Marinoni, Francesca Marongiu, Fausta Matera, Paolo Moretti, Laura Olimpi, Angela Pasinato, Ilaria Porro, Ippolita Roncoroni, Raffaella Schiro’, Patrizia Seppia, Federica Zanetto, Anna Aloisio, Elisabetta Anedda, Giuliana Apuzzo, Giovanna Argo, Anna Armenio, Emanuela Ballerini, Monica Benedetti, Daniela Bertoli, Stefano Bollettini, Chiara Bottalico, Aurora Bottiglieri, Vincenza Briscioli, Antonella Bruno, Laura Brusadin, Mariantonietta Caiazzo, Patrizia Calamita, Miriana Callegari, Rosaria Cambria, Rossella Claudia Cannavò, Maria Cristina Cantù, Domenico Capomolla, Anna Caracciolo, Maria Concetta Carbone, Gaetano Carrassi, Maria Laura Cartiglia, Sara Casagranda, Ornella Castiglione, Rosario Salvatore Cavallo, Teresa Cazzato, Maria Angela Cazzuffi, Melania MariaGiuseppina Cera, Jennifer Chiarolanza, Rosaria China, Nicoletta Cimadamore, Roberto Cionini, Cristina Ciuffo, Damiano Colazzo, Anna Maria Costantini, Claudio Cravidi, Marialuisa Criscione, Rita D’Agostino, Daniela Danieli, Luigi De Carlo, Marina De Sanctis, Giuseppina De Santes, Gian Piero Del Bono, Maria Elisabetta Di Pietro, Maria Chiara Dini, Paolo Fiammengo, Micaela Foco, Maria Teresa Fonte, Maria Frigeri, Andrea Galvagno, Matteo Gaudino, Stefania Genoni, Silvia Girotto, Emma MariaLetizia Giugnini, Gianluca Gornati, Marta Gozzi, Enrica Heritier, Antonella Lavagetto, Raffaele Limauro, Alessandra Magnelli, Maria Gabriella Maiolino, Monica Malventano, Silvia Marchi, Natale Maresca, Federico Marolla, Agata Martinelli, Chiara Martinez, Nicoletta Mascarello, Carla Matiotti, Elisabetta Mazzucchi, Donatella Moggia, Manuela Musetti, Paolo Nardini, Alberto Neri, Patrizia Neri, Flavia Nicoloso, Laura Maria Olimpi, Giancarlo Ottonello, Giacinta Padula, Paolo Maria Paganuzzi, Rosanna Palazzi, Alessandra Palmero, Maria Chiara Parisini, Giovannina Pastorelli, Marilena Pavoni, Lucia Peccarisi, Antonella Pellacani, Cristina Perrera, Michela Picciotti, Ivo Picotto, Tiziana Piunti, Francesca Preziosi, Giuseppe Primavera, Miriam Prodi, Maria Letizia Rabbone, Innocenza Rafele, Laura Reali, Franziska Stefanie Rempp, Ada Riundi, Paolo Rosas, Annarita Russo, Mariagrazia Saccà, Elisabetta Sala, Francesca Sala, Francesca Santus, Vittoria Sarno, Alessandra Savino, Raffaella Schirò, Giuseppa Scornavacca, Giovanni GiulianoSemprini, Maria Francesca Siracusano, Adelisa Spalla, Gloria Sturaro, Maria Grazia Toma, Ettore Tomagra, Maria Tortorella, Fausta Trentadue, Marina Trevisan, Silvia Tulisso, Roberta Usella, Anna Valente, Mariangela Valera, Edda Vernile, Valeria Vicario, Lucia Vignutelli, Paolo Vinci, Lucia Vizziello, Rosette Zand, Marco Zanette, Graziano Zucchi, and Maria Luisa Zuccolo

Subjects
Medicine
Abstract

Purpose The NASCITA Study, a national-level, population-based, prospective cohort study, was set up to better understand the early health status of Italian children, comprising their physical, cognitive and psychological development, and how it is affected by social and health determinants, including nurturing care. NASCITA will also assess geographical differences and disparities in healthcare.Participants Participating family paediatricians from throughout Italy enrolled infants born during the enrolment period (April 2019–July 2020). The 5054 newborns seen by the 139 paediatricians for at least two visits, including the first well-child visit, and for whom parental consent was given, make up the baseline population.Findings to date Mothers had a mean age at delivery of 33.0 years and tended to have a high or medium level of education (42.5% university and 41.7% high school degrees) and to be employed (69.7%). One-third (36.1%) took folic acid supplementation appropriately, and 6.5% smoked or consumed alcohol (10.0%) during pregnancy. One-third (31.7%) of deliveries were caesarean deliveries. Concerning the newborns, 5.8% had a low birth weight and 6.2% were born prematurely. The majority (87.7%) slept in the supine position, and 63.6% were exclusively breast fed at 1 month, with a decreasing north to south prevalence (χ2t 52; p

Published
2022
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4. High energy shock waves enhance the cytotoxic effect of doxorubicin and methotrexate to human osteosarcoma cell lines

Author

Ivana Ferrero, Roberto Frairia, Laura Berta, Alessandra Palmero, Franca Fagioli, Enrico Madon, Claudio Venturi, Massimo Berger, and Deborah Rustichelli

Subjects
Cancer Research, Pathology, medicine.medical_specialty, Cell, Antineoplastic Agents, Apoptosis, Bone Neoplasms, Biology, High-Energy Shock Waves, Cell Line, Tumor, medicine, Humans, Doxorubicin, HESW, shock waves, High energy shock waves, Cell Proliferation, Osteosarcoma, Cell growth, General Medicine, Cell cycle, medicine.disease, medicine.anatomical_structure, Methotrexate, Oncology, Cell culture, Cancer research, medicine.drug
Abstract

Despite recent advances, the prognosis of relapsed osteosarcoma patients remains very poor. Application of high energy shock waves may change the tumour cell growth and increase the cytotoxic effect of in vivo and in vitro chemotherapeutic agents. We studied the effect of their association with doxorubicin or methotrexate on three in vitro osteosarcoma cell lines. The effect of these combinations on SJSA-1, MG-63 and HOS human osteosarcoma cell lines were evaluated through incubation with doxorubicin or methotrexate and high energy shock wave treatment with 1000 shots at 0.22 mJ/mm(2) and an evaluation of the cell number, cell proliferation and apoptosis at days 1, 3 and 6 from the start of treatment. The combination of high energy shock waves and doxorubicin induced a statistically significant advantage in terms of a decrease in cell number on the SJSA-1 and HOS cell lines, a decrease of cell proliferation on all three cell lines and an increase of apoptosis only on the SJSA-1 cell line. The combination of high energy shock waves with methotrexate induced a decrease of the cell number only in the SJSA-1 and in the HOS cell lines, of the cell proliferation in the SJSA-1 and in the MG-63 cell lines, and an increase of apoptosis only on the SJSA-1 cell line. In conclusion, these experiments show an interesting effect of high energy shock waves on osteosarcoma cell lines, which could lead to future studies of the in vivo effects of high energy shock waves in the murine model as well.

Published
2006

5. Feasibility of cord blood stem cell manipulation with high-energy shock waves: an in vitro and in vivo study

Author

Enrico Madon, Claudio Venturi, Wanda Piacibello, Massimo Berger, Massimo Aglietta, Ymera Pignochino, Laura Berta, Fiorella Sanavio, Alessandra Palmero, Roberto Frairia, and Franca Fagioli

Subjects
Cancer Research, Population, Transplantation, Heterologous, CD34, Cell Culture Techniques, Antigens, CD34, Mice, SCID, Biology, CD38, Progenitor Cell Engraftment, High-Energy Shock Waves, Andrology, Mice, Mice, Inbred NOD, Genetics, Animals, Humans, Progenitor cell, education, Molecular Biology, Cell Proliferation, education.field_of_study, Graft Survival, Hematopoietic Stem Cell Transplantation, Dose-Response Relationship, Radiation, Cell Biology, Hematology, Fetal Blood, Hematopoietic Stem Cells, Transplantation, Cell culture, Cord blood, Immunology, Feasibility Studies
Abstract

Objective Cord blood CD34 + cells are more uncommitted than their adult counterparts as they can be more easily maintained and expanded in vitro and transduced with lentiviral vectors. The aim of this study was to evaluate whether pretreatment with high-energy shock waves (HESW) could further enhance the expansion of cord blood progenitors and the transduction efficiency with lentiviral vectors. Methods Human cord blood CD34 + cells underwent HESW treatment with a wide range of energy and number of shots (from 0.22 mJ/mm 2 to 0.43 mJ/mm 2 and from 200 to 1500 shots). Cells were then evaluated both for their in vitro expansion ability and in vivo engraftment in primary, secondary, and tertiary NOD/SCID mice. The transduction efficiency with a lentiviral vector (LV) was also evaluated in vitro and in vivo. Results Cell viability following HESW ranged from 75 to 92%. Pretreatment with HESW significantly improved early progenitor cell expansion after short-term suspension culture. Upon transplantation in primary NOD/SCID mice, the HESW treatment enhanced progenitor cell engraftment (total human CD45 + CD34 + cells were 10% in controls and 14.5% following HESW, human CD45 + CD34 + CD38 − cells were 0.87% in controls and 1.8% following HESW). HESW treatment enhanced the transduction of a GFP + lentiviral vector (e.g., at day 42 of culture 6.5% GFP + cells in LV-treated cell cultures compared to 11.4% of GFP + cells in HESW-treated cell cultures). The percentage of human GFP + cell engrafting NOD/SCID mice was similar (34% vs 26.4% in controls); however, the total number of human cells engrafted after HESW was higher (39.6% vs 15%). Conclusion The pretreatment of CD34 + cells with HESW represents a new method to manipulate the CD34 + population without interfering with their ability to both expand and engraft and it might be considered as a tool for genetic approaches.

Published
2004

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