Search

A large number of apex predator samples are available in European research collections, environmental specimen banks and natural history museums that could be used in chemical monitoring and regulation. Apex predators bioaccumulate pollutants and integrate contaminant exposure over large spatial and temporal scales, thus providing key information for risk assessments. Still, present assessment practices under the different European chemical legislations hardly use existing chemical monitoring data from top predators. Reasons include the lack of user-specific guidance and the fragmentation of data across time and space. The European LIFE APEX project used existing sample collections and applied state-of-the-art target and non-target screening methods, resulting in the detection of > 4,560 pollutants including legacy compounds. We recommend establishing infrastructures that include apex predators as an early warning system in Europe. Chemical data of apex species from freshwater, marine and terrestrial compartments should become an essential component in future chemical assessment and management across regulations, with the purpose to (1) validate registration data with ‘real world’ measurements and evaluate the predictability of current models; (2) identify and prioritise hazardous chemicals for further assessment; (3) use data on food web magnification as one line of evidence to assess biomagnification; (4) determine the presence of (bio)transformations products and typical chemical mixtures, and (5) evaluate the effectiveness of risk management measures by trend analysis. We highlight the achievements of LIFE APEX with regard to novel trend and mixture analysis tools and prioritisation schemes. The proposed advancements complement current premarketing regulatory assessments and will allow the detection of contaminants of emerging concern at an early stage, trigger risk management measures and evaluations of their effects with the ultimate goal to protect humans and the environment. This is the second policy brief of the LIFE APEX project.

Overwhelming evidence over many decades has shown that many organic compounds have been released in the environment due to anthropogenic activities. The term “chemicals of emerging concern (CEC)” has been established for chemicals, which are not subjected to marketing restrictions and regulatory monitoring programmes but are candidates for future regulation, due to their frequent detection in environmental samples and their potential hazardous properties (Thomaidis et al, 2012; Gavrilescu et al, 2015; Dulio et al, 2018). Despite the fact that their chemical and physical properties allow them to enter marine, freshwater and/or terrestrial ecosystems, data for the potential bioaccumulation and biomagnification of CECs though the food are missing, which increases the concern about their effects on the ecosystems, biota and human health. CECs, in many studies, are determined by targeted methods, based on the analysis of reference standards (e.g., plant protection products, OCPs, PAHs, PFAS, pharmaceuticals and veterinary medicines). However, despite the high selectivity and sensitivity of targeted methods, using mainly selected reaction monitoring (SRM) mode of detection, they are limited to the determination of a restricted number of compounds. On the other hand, High Resolution Mass Spectrometry (HRMS) techniques, given the high resolution capability (R=35,000) and mass accuracy in full scan mode, have enabled the simultaneous determination of hundreds of emerging contaminants and their transformation products, even if reference standards are not commercially available (Picó et al, 2012; Du et al, 2017). Although thousands of chemicals are marketed in Europe, so far only 600 chemicals have been screened and identified as PBT (persistent, bioaccumulative, toxic), ED (endocrine disrupting) and/or CMR (carcinogenic, mutagenic, toxic for reproduction), because human and environmental hazard assessment is laborious and often obstructed due to the lack of data (ECHA’s 2016 General Report, ECHA 2017). The determination of contaminants in apex predators and their prey (AP&P) allows to identify the emergence of chemicals including substitutes of regulated compounds which may present similar levels of hazard. Apex predators play a key role in the monitoring of environmental contaminants and in risk assessment studies for a number of reasons including: their position at the top of food webs, a relatively long lifespan over which to accumulate contaminants, integration of exposure both over time and relatively large spatial areas, relative ease with which samples can be obtained, and relative ease with which populations can be quantified and monitored (Movalli et al, 2017). The EU funded LIFE APEX project (LIFE17 ENV/SK/000355, 2018-2022), demonstrates the use of AP&P in monitoring contaminants in the environment and assessing the effectiveness of chemicals risk management measures under EU regulations. The aim of LIFE APEX (https://www.lifeapex.eu) is to improve systematic use, by regulators, of chemical monitoring data from apex predators (Harbour Seal, Common Buzzard, Eurasian Otter) and their prey (freshwater fish: Bream/Roach, marine fish: Eelpout/Herring) for better chemicals management, thereby reducing exposure to harmful substances and protecting human health and the environment. LIFE APEX links biota samples from different sample collections with novel analytical methods, for a more thorough understanding of chemicals’ occurrence and bioaccumulation through the food chain. These data can be used for a better prioritization of hazardous substances, their regulation and the effectiveness evaluation of chemicals management. For revealing the presence of CECs in AP & P samples, during the first year of LIFE APEX implementation, 67 recent samples (2015-2019) of apex predators (livers) & their prey (muscles) from four Northern Europe’s countries (United Kingdom, Germany, Netherlands and Sweden), from Environmental Specimen Banks (ESB’s), Natural History Museums (NHM’s) and other scientific collections, were analyzed following state-of-the-art wide-scope target screening methodologies. All samples were lyophilized before analysis, in order to enhance extraction efficiency, improve the precision and achieve lower detection limits. The analytes were extracted from the dry matrices through generic methods of extraction, using Accelerated Solvent Extraction (ASE), followed by a clean-up step using Solid Phase Extraction. The final extracts were analyzed by different chromatographic and ionization techniques (both liquid and gas chromatography) coupled to High Resolution Mass Spectrometry (using electrospray and atmospheric pressure ionization, respectively), in order to broaden the chemical domain accessible to wide-scope target analysis. The samples were screened for the presence of more than 2,400 organic pollutants, included in the target list of the University of Athens (UoA). UoA database includes compounds of different classes (such as pharmaceuticals, personal care products, biocides, plant protection products, illicit drugs, stimulants, sweeteners, and industrial chemicals, e.g. per- and polyfluorinated compounds (PFASs), flame retardants, corrosion inhibitors, plasticizers, surfactants), as well as their transformation products and metabolites. The target list is being updated on a regular basis, since new compounds of concern are continuously being identified. Strict criteria of mass accuracy (

Monitoring data from apex predators were key drivers in the development of early chemicals legislations due to the population declines of many species during the twentieth century, which was linked to certain persistent organic pollutants (POPs). Besides triggering the development of global treaties (e.g. the Stockholm Convention), chemical monitoring data from apex predators have been particularly important for identifying compounds with bioaccumulative properties under field conditions. Many apex predators are protected species and only a few environmental specimen banks (ESBs) regularly collect samples as many ESBs were established during the 1980–1990s when apex predators were scarce. Today, many POPs have been banned, which contributed to the recovery of many apex predator populations. As a consequence, apex predator samples are now available in research collections (RCs) and natural history museums (NHMs). These samples can be used for routine analysis as well as for screening studies using novel analytical techniques and advanced data treatment workflows, such as suspect and non-target screening. The LIFE APEX project has demonstrated how these samples can be used in a cost-efficient way to generate data on legacy compounds and contaminants of emerging concern. Furthermore, it has described quality assurance/control measures to ensure high quality and comparable data, with a view to uses in chemicals risk assessment and management. To increase the visibility of available sample collections and monitoring data from apex predators we developed accessible online database systems. Additionally, the acquired high-resolution mass spectrometric data were stored in a digital sample freezing platform that allows retrospective suspect screening in previously analysed samples for substances that may be of concern/under assessment in the future. These databases provide open access to a wide range of chemical data, for use by regulators, researchers, industry and the general public, and contribute to a stronger link between science and policy.

Due to their spread, abundance and potential impact on food security and human health, microplastics (MPs) are emerging global pollutants. Metropolitan areas are among the main sources of MPs (1 μm - 5 mm); indeed, about 80% of the MPs found in the oceans come from freshwaters. In particular, impervious surfaces runoff in urban areas results in the transport of large quantities of solid wastes, comprising MPs, to the superficial water bodies. Thus, the ecological state of urban streams represents a reliable indicator to evaluate the environmental impact of a city. In this study, we report data about MPs in stream sediments and waters of a minor urban stream, the Mugnone Creek (MC), which flows across the highly urbanized city of Florence (Italy) and discharges to the Arno River.Several sites along the 17 km-long MC were chosen, including “greenfield” sites upstream of the Florence urban area, urban-impacted sites located along congested roads, and the MC outlet. The stream sediments were collected in June 2019, while stream waters were recovered via glass bottles twice a year (June and December) in 2019 and 2020, to account for seasonal variability. Stream discharge was simultaneously determined during water sampling to allow mass flow calculations of contaminants.Water samples were filtered onto glass microfiber filters (ø 47 mm) and observed by HD digital stereomicroscope; a similar method was followed for sediments after a density separation step (NaCl saturated solution) and H2O2 digestion. Fourier Transform Infrared Spectroscopy (FT-IR) was used for identification and characterization of MPs. Microparticles classification was based on polymer type, shape and colour.MPs concentration in sediments showed an increasing trend from the pre-urban site to the outlet. A maximum value (1.540 MPs/kg) was reached immediately after the Terzolle Creek confluence, which drains the large University Hospital District of Careggi. Fibers were the dominant shape class of polymers observed and blue/black items stand out among the colour classes. The highest concentrations of MPs in water samples were recorded during winter seasons (up to 16.000 items/m3), with a predominance of fibers and blue/black colours. Polymer classification by FTIR indicated the presence of (in order of abundance): PA (polyamide), PET (Polyethylene Terephthalate), SBR (butadiene-styrene rubber), PP (Polypropylene), blend PP+PE (PP+Polyethylene), PTFE (Polytetrafluoroethylene) and PU (Polyurethane). The black-SBR polymers likely related to tyre abrasion occurring during vehicles driving, since they were especially found on a site close to traffic-congested roads. In addition to synthetic particles, high concentrations of natural fibers (mainly cellulose) were found in waters at all sites. Up to 109 synthetic particles are estimated to be discharged daily by MC to the Arno River during the winter season, a load much higher than creeks with similar urbanization context worldwide. Mass loads of natural fibers were of the same order of magnitude of MPs in every season.Studies are in progress to elucidate the impact on local biota and to characterize the anthropic pressure on the Arno River, aiming to improve the knowledge about the environmental status of one of the main Italian river basins.

Indoor Air Quality monitoring in cultural institutions is of particular concern to protect these places and the cultural heritage content. An indoor monitoring campaign was performed in three museums in Florence (Italy) to determine the occurrence and levels of volatile organic compounds (VOCs). VOCs of interest included BTEX (benzene, toluene, ethylbenzene, xylenes), terpenes, aldehydes, organic acids, and cyclic volatile methyl siloxanes (cVMS). The most abundant VOCs in all samples analyzed were BTEX, which were strictly related to the traffic source, followed by siloxanes and terpenes. Among BTEX, toluene was always the most abundant followed by xylenes, ethylbenzene, and benzene. cVMS in exhibition rooms with the presence of visitors showed higher values compared to samples collected when the museums were closed. Terpenes showed not only the influence of vegetation-biogenic sources surrounding a museum but could also be related to the wood used for the construction of showcases and furniture and the use of cleaning products. Data obtained also showed the presence of organic acids and aldehydes whose source can be traced back to exhibits themselves and wood-based furniture. Assessing the levels of organic acids in museums is important because, over time, it can cause deterioration of the artifacts.

Movalli, Paola et al., The chemical industry is the leading sector in the EU in terms of added value. However, contaminants pose a major threat and significant costs to the environment and human health. While EU legislation and international conventions aim to reduce this threat, regulators struggle to assess and manage chemical risks, given the vast number of substances involved and the lack of data on exposure and hazards. The European Green Deal sets a ‘zero pollution ambition for a toxic free environment’ by 2050 and the EU Chemicals Strategy calls for increased monitoring of chemicals in the environment. Monitoring of contaminants in biota can, inter alia: provide regulators with early warning of bioaccumulation problems with chemicals of emerging concern; trigger risk assessment of persistent, bioaccumulative and toxic substances; enable risk assessment of chemical mixtures in biota; enable risk assessment of mixtures; and enable assessment of the effectiveness of risk management measures and of chemicals regulations overall. A number of these purposes are to be addressed under the recently launched European Partnership for Risk Assessment of Chemicals (PARC). Apex predators are of particular value to biomonitoring. Securing sufficient data at European scale implies large-scale, long-term monitoring and a steady supply of large numbers of fresh apex predator tissue samples from across Europe. Natural science collections are very well-placed to supply these. Pan-European monitoring requires effective coordination among field organisations, collections and analytical laboratories for the flow of required specimens, processing and storage of specimens and tissue samples, contaminant analyses delivering pan-European data sets, and provision of specimen and population contextual data. Collections are well-placed to coordinate this. The COST Action European Raptor Biomonitoring Facility provides a well-developed model showing how this can work, integrating a European Raptor Biomonitoring Scheme, Specimen Bank and Sampling Programme. Simultaneously, the EU-funded LIFE APEX has demonstrated a range of regulatory applications using cutting-edge analytical techniques. PARC plans to make best use of such sampling and biomonitoring programmes. Collections are poised to play a critical role in supporting PARC objectives and thereby contribute to delivery of the EU’s zero-pollution ambition., This paper was carried out in collaboration between ‘European Raptor Biomonitoring Facility’ (COST Action 16224), and the project ‘LIFE APEX—Systematic use of contaminant data from apex predators and their prey in chemicals management’ (LIFE17 ENV/SK/000355). European Raptor Biomonitoring Facility is supported by COST (European Cooperation in Science and Technology) and funded by the Horizon 2020 Framework Programme of the European Union. LIFE APEX is funded by the LIFE Programme, the EU’s funding instrument for the environment and climate action.

Per- and polyfluoroalkyl substances (PFAS) are a group of emerging substances that have proved to be persistent and highly bioaccumulative. They are broadly used in various applications and are known for their long-distance migration and toxicity. In this study, 65 recent specimens of a terrestrial apex predator (Common buzzard), freshwater and marine apex predators (Eurasian otter, harbour porpoise, grey seal, harbour seal) and their potential prey (bream, roach, herring, eelpout) from northern Europe (United Kingdom, Germany, the Netherlands and Sweden) were analyzed for the presence of legacy and emerging PFAS, employing a highly sensitive liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method. 56 compounds from 14 classes were measured; 13 perfluoroalkyl carboxylic acids (PFCAs), 7 perfluoroalkyl sulphonic acids (PFSAs), 3 perfluorooctane sulfonamides (FOSAs), 4 perfluoroalkylphosphonic acids (PFAPAs), 3 perfluoroalkylphosphinic acids (PFPi's), 5 telomer alcohols (FTOHs), 2 mono-substituted polyfluorinated phosphate esters (PAPs), 2 di-substituted polyfluorinated phosphate esters (diPAPs), 6 saturated fluorotelomer acids (FTAS), 3 unsaturated fluorotelomer acids (FTUAs), 2 N-Alkyl perfluorooctane sulfonamidoethanols (FOSEs), 3 fluorotelomer sulphonic acids (FTSAs), 2 perfluoroether carboxylic acids (PFECAs) and 1 chlorinated perfluoroether sulphonic acid (Cl-PFESA). All samples were lyophilized before analysis, in order to enhance extraction efficiency, improve the precision and achieve lower detection limits. The analytes were extracted from the dry matrices through generic methods of extraction, using an accelerated solvent extraction (ASE), followed by clean-up through solid phase extraction (SPE). Method detection limits and method quantification limits ranged from 0.02 to 1.25 ng/g wet weight (ww) and from 0.05 to 3.79 ng/g (ww), respectively. Recovery ranged from 40 to 137%. Method precision ranged from 3 to 20 %RSD. The sum of PFAS concentration in apex predators livers ranged from 0.2 to 20.2 μg/g (ww), whereas in the fish species muscle tissues it ranged from 16 to 325 ng/g (ww). All analyzed specimens were primarily contaminated with PFOS, while the three PFPi's included in this study exhibited frequency of appearance (FoA) 100 %. C9 to C13 PFCAs were found at high concentrations in apex predator livers, while the overall PFAS levels in fish fillets also exceeded ecotoxicological thresholds. The findings of our study show a clear association between the PFAS concentrations in apex predators and the geographical origin of the specimens, with samples that were collected in urban and agricultural zones being highly contaminated compared to samples from pristine or semi-pristine areas. The high variety of PFAS and the different PFAS composition in the apex predators and their prey (AP&P) samples is alarming and strengthens the importance of PFAS monitoring across the food chain.

Microplastics (MPs) and textile cellulose are globally pervasive pollutants in freshwater. In-situ studies assessing the ingestion of MPs by freshwater meiofauna are few. Here, we evaluated MP and textile cellulose ingestion by some meiofaunal taxa and functional guilds of a first-order stream in the city of Florence (Italy) by using a tandem microscopy approach (fluorescence microscopy and μFTIR). The study targeted five taxa (nematodes, oligochaetes, copepods, ephemeropterans and chironomids), three feeding (scrapers, deposit-feeders, and predators), and three locomotion (crawlers, burrowers, and swimmers) guilds. Fluorescent particles related to both MPs and textile cellulose resulted in high numbers in all taxa and functional guilds. We found the highest number of particles in nematodes (5200 particles/ind.) and deposit-feeders (1693 particles/ind.). Oligochaetes and chironomids (burrowers) ingested the largest particles (medium length: 28 and 48 μm, respectively), whereas deposit-feeders ingested larger particles (medium length: 26 μm) than scrapers and predators. Pellets were abundant in all taxa, except for Chironomidae. Textile cellulose fibers were present in all taxa and functional guilds, while MP polymers (EVA, PET, PA, PE, PE-PP) differed among taxa and functional guilds. In detail: EVA and PET particles were found only in chironomids, PE particles occurred in chironomids, copepods and ephemeropterans, PA particles were found in all taxa except in nematodes, whereas particles made of PE-PP blend occurred in oligochaetes and copepods. Burrowers and deposit-feeders ingested EVA, PET, PA, PE and PE-PP, while crawlers and scrapers ingested PE and PA. Swimmers and predators ingested PE, PA and PE-PP. Our findings suggest a pervasive level of plastic and textile cellulose pollution consistent with an urban stream which propagates in the meiofaunal assemblage of the stream ecosystem.

Using monitoring data from apex predators for chemicals risk assessment can provide important information on bioaccumulating as well as biomagnifying chemicals in food webs. A survey among European institutions involved in chemical risk assessment on their experiences with apex predator data in chemical risk assessment revealed great interest in using such data. However, the respondents indicated that constraints were related to expected high costs, lack of standardisation and harmonised quality criteria for exposure assessment, data access, and regulatory acceptance/application. During the Life APEX project, we demonstrated that European sample collections (i.e. environmental specimen banks (ESBs), research collection (RCs), natural history museums (NHMs)) archive a large variety of biological samples that can be readily used for chemical analysis once appropriate quality assurance/control (QA/QC) measures have been developed and implemented. We therefore issued a second survey on sampling, processing and archiving procedures in European sample collections to derive key quality QA/QC criteria for chemical analysis. The survey revealed great differences in QA/QC measures between ESBs, NHMs and RCs. Whereas basic information such as sampling location, date and biometric data were mostly available across institutions, protocols to accompany the sampling strategy with respect to chemical analysis were only available for ESBs. For RCs, the applied QA/QC measures vary with the respective research question, whereas NHMs are generally less aware of e.g. chemical cross-contamination issues. Based on the survey we derived key indicators for assessing the quality of biota samples that can be easily implemented in online databases. Furthermore, we provide a QA/QC workflow not only for sampling and processing but also for the chemical analysis of biota samples. We focussed on comprehensive analytical techniques such as non-target screening and provided insights into subsequent storage of high-resolution chromatograms in online databases (i.e. digital sample freezing platform) to ultimately support chemicals risk assessment.

The composition and distribution of microplastics (MPs) in the Brazilian Amazon Continental Shelf surface waters are described for the first time. The study was conducted during the 2018 rainy and dry seasons, using 57 water samples collected with aluminum buckets and filtered through a 64-μm mesh. The samples were vacuum-filtered in a still-air box, and the content of each filter was measured, counted, and classified. A total of 12,288 floating MPs were retrieved; particles were present at all 57 sampling points. The mean MP abundance was 3593 ± 2264 items·m

The Victoria Land (East Antarctica) is characterized by the presence of lakes and ponds where rare water is found during the Summer months. These freshwater ecosystems are an important resource for migrating seabirds that use them and leave there their droppings and feathers, contributing to enrich the water with organic matter. Persistent organic pollutants (POPs) are long-range transported to polar regions and their detection in the Antarctic ecosystems date back to the 1960s. Most studies have been related to POP concentrations in marine environment, and terrestrial ecosystems have been poorly investigated. This study reports the concentration of PBDEs (23 congeners) and PCBs (21 among non-, mono-, di-, and ortho congeners) in lake sediment, soil and vegetation mat (community of algae, cyanobacteria, bryophites) collected close to six lakes in the Victoria Land (74°31'S-74°97'S, 165°07'E-162°51'E): Edmonson Point 14 and 15A, Carezza, Enigma, Tarn Flat 20, Inexpressible Island 10B. The ∑PBDEs averaged 0.09-0.28 ng/g and BDEs 28, 47 and 154 were higher in mat and soils, while BDEs 183 and 47 in sediment samples. PCBs ranged0.003-0.807 ng/g and congeners nos. 114, 138, and 187 were the most abundant. In addition, TEQs were derived for the non-and mono-ortho PCBs detected and values were very low in each matrix (0.010 pg/g in the soil, 0.012 pg/g in sediment and mat). The long-range atmospheric transport can be confirmed as the most important POP source in Antarctica, although the scientific stations and seabird colonies may be potential local sources and contribute to contaminant release.

Water quality assessment is vital to identify existing problems and any changes that emerge in water sources over a period of time. Conventional water quality monitoring systems remain to be limited to on-site sample collection and further analysis in environmental laboratories. The progress in Arduino-based low-cost and open-source hardware has paved the way for the development of low-cost, portable, and on-site measuring platforms. In this work, we have assembled an Arduino-based open-source water testing platform out of commercially available sensors and controllers. The water testing system was powered by a 9 V battery and had the capability of measuring water turbidity, acidity, and temperature on-site in real-time. The calibration and validation studies were carried out to assess the measurement capabilities of turbidity and pH sensors in the lab using calibration samples and UV-Vis-NIR absorption spectroscopy. The water quality platform was tested in an artificial lake that is located at Sabanci University Campus (Istanbul, Turkey), which serves as a reservoir for treated wastewaters and rainwater. Untreated wastewater samples were collected from the wastewater treatment station of the university for comparison. The measurements performed on several locations along the coast of the artificial lake were also validated in the laboratory. The water testing platform showed significant potential for miniaturization and portability of such analytical platforms for on-site environmental monitoring.

The Hooghly River (HR) estuary is the first deltaic off-shoot of the perennial and transboundary river, Ganga, India. HR receives industrial and domestic waste along with storm-water run-off from Kolkata city and the adjoining districts. Organic micropollutants (OMPs) have been collectively termed for plasticizers, pharmaceuticals and personal care products, which are extensively consumed and disposed in the waste streams. Hence emerging OMPs were investigated to obtain the first baseline data from the Hooghly riverine sediment (HRS) along urban and suburban transects using gas chromatography mass spectrometry (GC-MS). The concentration range of OMPs in the HRS varied between 3 and 519 ng/g for carbamazepine, 5–407 ng/g for non-steroidal anti-inflammatory drugs (NSAIDs), 2–26 ng/g for musk ketone, 2–84 ng/g for triclosan, 2–199 ng/g for bisphenol A (BPA), 2–422 ng/g for plasticizers (phthalic acid esters (PAEs) and bis (2-ethylhexyl) adipate (DEHA)) and 87–593 ng/g for parabens. Carbamazepine concentration in sediment was an useful marker for untreated wastewater in urban waterways. High concentrations of BPA and PAEs in the suburban industrial corridor together with significant correlation between these two type of OMPs (r2 = 0.5; p

The review aimed to evaluate the sustainability of wood biomass burning for energy production and its contribution to the air quality and impact to human health in different European cities and to highlight the most important steps to lower particulate aerosol emissions. The contribution of residential wood combustion resulted to be relevant to the particulate matter (PM) mass, with great discrepancies in biomass burning contribution to PM mass in European cities due to the different investigated seasons and different degree by the use of wood as fuel for residential heating. The importance of source apportionment of atmospheric aerosol for the implementation of effective strategies to control PM emissions and assess health effects due to air quality was also evidenced. Two major issues emerged from the critical literature analysis: the need of high-quality emission inventories and European policy strategies, such as replacement of old burning devices with improved lower emission ones.

People spend more than 90% of their time indoors and a very big part of that time inside their bedrooms, suggesting that the quality of the air that people breathe during sleeping is of particular importance and can play an important role in our overall exposure to chemicals and also in our well-being. The bedrooms' air quality is, according to the authors’ opinion, rather neglected in most studies, and with this opinion article, we try to bring this research question into more attention of the scientific community and also of the society and public authorities. Even though there are always more articles dealing with indoor air quality (IAQ) and new or emerging chemicals, the number of studies that address the IAQ in bedrooms is not proportionally increasing, let alone the fact that even studies that monitor air quality in bedrooms sometimes do not focus on the IAQ during the sleeping periods to simulate exactly what are the IAQ problems during an entire night. Herein, we explain why, in our opinion, the air quality in bedrooms should be addressed in a different way than other microenvironments and why this kind of research should be further intensified to help authorities and environmental agencies correctly assess the exposure of individuals to indoor toxic pollutants.

This review provides insight into the abundance, origin, distribution and composition of MPs in the sea surface and water column of the Mediterranean Sea. Literature data on MP particles on the sea surface showed an evident heterogeneous distribution and composition, with marked geographical differences between Mediterranean sub-basins. A standardized protocol for water sampling, extraction and detection of plastic debris is strongly recommended. The heterogenicity of MPs distribution and its concentration levels could be related to several factors, such as the different methodological approaches. In addition, the influence of hydrodynamic features such as currents, up and down-welling, gyres and fronts could also be responsible for this heterogeneity in concentrations. Marine litter modelling studies have been applied to understand litter sources, fate, transport and accumulation in oceans. Recent studies focused on the “plastisphere” in order to better understand the potential risk of pathogen dispersion with plastic transport in the Mediterranean Sea.

The Mediterranean Sea is affected by one of the most significant plastic pollution worldwide. This review critically evaluates the most recent literature on the presence of microplastics in sediments, suggested to be long term sinks and have a high potential to accumulate this kind of marine debris. A picture of microplastic levels in coastal environments is given, evidencing information gaps and considering also estuary, lagoons and areas influenced by the contribution of rivers. A wide range of contamination levels has been found, with the highest in lagoon and estuary environments. The lack of homogeneity in the methods of study and the need to harmonize the latter and the expression of the results in addition to the need to obtain data on the contributions of the main tributaries of the Mediterranean and on lagoons, are other important considerations taken.

Urbanization is a striking phenomenon, responsible for the development of cities as complex and highly dynamic systems. One of the most pressing issues in urban areas is water cycle management, which directly influences the availability and the quality of this resource. Urban streams are highly vulnerable to the impacts resulting from the increasing urbanization and they have often lost most of their pristine, natural character.We hereby present the preliminary results of the multidisciplinary StrEams urbaN Ecological City plAnning (SENECA) project, which is not only but mainly devoted to characterizing the chemical features of stream waters and sediments of the Mugnone Creek (MC). MC is a typical example of an urban stream, crossing the city of Florence (Italy) and eventually discharging to the Arno River, that has suffered an intense denaturation due to the urban sprawl, such as several diversions, canalizations and rectifications, alterations of the stream bed and riparian habitat, widespread concrete revetment of the banks and burial of short stream tracts.Different sites along the 17-km long MC were investigated for water and sediment geochemistry from upstream of the Florence urban area (“blank” sites) to the MC outlet (Cascine Park), passing through variably urban-impacted areas, including sites located along traffic-congested roads, close to the new tramway construction sites and under the railway line. Stream waters were sampled twice a year (June and December 2019 and 2020) to account for seasonal variability. Stream discharge at three representative points was simultaneously determined during water sampling to allow mass load calculations of contaminants. In details, major dissolved ions (Ca, Mg, Na, K, HCO3, SO4, Cl, NO3) and reduced nitrogen (NH4), trace elements (TEs: Sb, As, Cu, Zn, Cd, Co, Ba, Li, Pb, Ni) and microplastics (MPs) in stream sediments and waters of MC were determined. Major dissolved ions and NH4 were analysed via acidimetric titration and IC. TEs were determined in both sediments and waters (0.45 μm filtered and unfiltered) by ICP-OES and ICP-MS, respectively. MPs (1 μm-5 mm) were identified in sediments and waters by HD binocular stereomicroscope.Chemical composition (major element constituents) of water reflected both the seasonality and the progress of the water-rock interaction acting in MC from its spring toward the outlet. In addition, the pattern of abundances of Cu, Zn, Sb and Pb and especially their mass loads reflect the increasing anthropogenic impact on the water and sediments, even if is important to stress that the contents are within the limits defined by law, except for stream sediments at a few sites. For MPs, up to 109 particles, coming mainly from the NW side of Florence, are estimated to be discharged daily to the Arno River during the winter season, much higher than creeks with similar urbanization contexts worldwide. Increasing data collection will make it possible to identify and better understand what type of sources and processes are responsible for the anthropogenic impacts in the MC and could help in better safeguarding the creek within the framework of the urban social-ecological systems of Florence.

Macro-, meso-and microplastic (MAP, MEP, MP) occurrence in compost is an environmental issue whose extent and effects are not yet understood. Here, we studied the occurrence of MAPs, MEPs and MPs in compost samples, and the transfer of hazardous contaminants from plastics to compost and soil. MAPs/MEPs and MPs concentrations in compost were 6.5 g/kg and 6.6 +/- 1.5 pieces/kg; from common recommendations for compost application, we estimated similar to 4-23 x 10(7) pieces MPs and 4-29 x 10(4) g MAPs/MEPs ha(-1) per year ending into agricultural soils fertilized with such compost. Regarding contaminants, bis(ethylhexyl) phthalate, acetyl tributyl citrate, dodecane and nonanal were extracted in higher concentrations from plastics and plastic-contaminated compost than from compost where MAPs/MEPs had been removed prior to extraction and analysis. However, some contaminants were present even after MAPs/MEPs removal, ascribable to short-and long-term release by MAPs/MEPs, and to the presence of MPs. DEHP concentration was higher in soils where compost was applied than in fields where it was not used. These results, along with estimations of plastic load to soil from the use of compost, show that compost application is a source of plastic pollution into agricultural fields, and that plastic might transfer hazardous contaminants to soil.

This study investigated the ingestion of microplastics and artificial cellulose particles by 103 specimens belonging to 21 reef fish species from the southwestern Atlantic. Specimens of six species had ingested microplastics and artificial cellulose particles, while those of another three species had ingested only one type of material. In our samples, man-made cellulose fibers were more common than microplastics. The tomtate grunt, Haemulon aurolineatum, ingested more particles than any of the other species. Overall, transparent particles were predominant, and polyamide was the most common plastic material. Household sewage, fishery activity, and navigation appear to be the principal sources of the artificial particles ingested by the reef fishes. Our results provide an important database on oceanic contamination by microplastics and artificial cellulose particles. Understanding this impact on tropical reef fish will contribute to the development of strategies to mitigate pollution by anthropogenic debris in reef systems.

Conversion of agricultural fields into the industrial corridor under the State Industries Promotion Corporation of Tamil Nadu Limited (SIPCOT) necessitated the investigation of soil-borne organic contaminants. This study is the first attempt to evaluate the occurrence of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in soils from Mambakkam and Cheyyar SIPCOT belt, along the residential, industrial and agricultural transects. Concentrations of Σ28PCBs, Σ16PAHs and OCPs were in the range 0.3–9 ng/g, 33–2934 ng/g and nd–81.4 ng/g, respectively. Residential areas showed higher OCP concentrations than other site types, probably due to their frequent use in vector control programmes. DDT isomers and α-isomer of endosulfan showed low concentrations indicating past usage of these OCPs. Principal component analysis indicated that high-temperature combustion and industrial processes might be the major sources of high molecular weight PAHs, while low-temperature combustion processes might be responsible for low molecular weight PAHs. PCBs in soil were probably attributed to unaccounted combustion processes of e-waste in the region. Carcinogenic PAHs and Σ28PCBs were higher in the industrial sites. Mean Σ28PCBs at Mambakkam (4.8 ng/g) was significantly higher (p

This study reports for the first time the ingestion of meso- (5.01-25 mm) and microplastics (1 μm-5 mm) by the sea anemone Bunodosoma cangicum, the most abundant actiniarian species on the Amazon coast. At three sites on the coast of Pará, Brazil, anemones were collected from beachrocks in the intertidal zone (30 at each site), measured (pedal disc diameter, mm) and weighed (wet weight, g). The contents of the gastrovascular cavity were extracted and analyzed under a stereoscope. The recovered plastic particles were characterized by Fourier Transform Infrared (FTIR) spectroscopy. Overall, 139 microplastic and 2 mesoplastic items were identified in 68 individuals (75.6%) among the 90 examined, with a mean of 1.6 (±1.5) items per individual. Plastic fibers comprised about 84% of the ingested plastics, followed by fragments (∼12%) and films (∼4%). Particle diameters ranged from 0.10 to 9.17 mm (1.57 ± 1.23 mm). A weak positive correlation was found between the weight of anemones and the number of plastic particles in the gastrovascular cavity (p = 0.03) and between the number of prey items and the number of plastic particles in the gastrovascular cavity (p 0.01). The main polymers identified by FTIR analysis were polyethylene terephthalate (PET), polypropylene (PP), polyamide (PA), polyurethane (PU), polyethylene (PE), acrylonitrile butadiene styrene (ABS), polystyrene (PS) and rayon. Sea anemones ingested significantly more plastic debris at the most urbanized and populous sampling sites. This study provides the first evidence of microplastics contamination of marine invertebrates from the Amazon coast. Abundant species such as B. cangicum have the potential to monitor the levels of plastic contamination in the region. Our results support this potential, as the species showed a high frequency of plastic ingestion and allowed detection of plastic contamination even in the best-preserved area where anemones were collected.

Co-composting is a technique that allows the aerobic degradation of organic waste mixtures, primarily aiming at obtaining compost that can be used as fertiliser or soil amendment. As compared to the typical composting activity, the main difference is not merely the use of more than one feedstock to start and sustain the biodegradation process, but also the possibility of combining various kinds of waste to obtain ‘tailored’ products with designed properties, or to reclaim and valorise natural resources, such as degraded soils or polluted soils and sediments. Set up of appropriate co-composting protocols can be a way to optimise the management of waste produced by different sectors of agriculture and industry and also from human settlements. Different formulations can not only optimise the biodegradation process through the adjustment of nutrient ratios, but also lead to the formation of products with innovative properties. Moreover, co-composting can be a technique of choice for the reclamation of soils degraded by intensive agriculture or contaminated soils and sediments. In fact, an appropriate mix of organic waste and soils can restore the soil structure and induce fertility in nutrient-depleted soils, and also remediate polluted soils and sediments through degradation of organic pollutants and stabilisation of heavy metals. While the selection of different mixes of organic waste may lead to the design of composts with specific properties and the potential valorisation of selected waste materials, there are still several factors that hamper the development of co-composting platforms, mainly insufficient knowledge of some chemical and microbiological processes, but also some legislative aspects. This chapter illustrates the progress achieved in co-composting technology worldwide, some key legislative aspects related to the co-composting process, the main scientific and technical aspects that deserve research attention to further develop co-composting technology, and successful applications of co-composting for the reclamation of soils and sediments, allowing their use for cultivation or as growing media in plant nurseries. A specific case study of the production of fertile plant-growing media from sediment co-composting with green waste is also illustrated.

Microlitter ( μ m porosity and black grid membranes and examined using a binocular microscope. Items were counted, dimensionally measured and classified according to type, shape, colour and appearance. A total of 56 torrent and sea sediment samples revealed the presence of 4,302 items. The mean concentrations were 1.5 items cm − 3 ± 1.3 standard deviation (SD) and 1.6 items cm − 3 ± 1.3 SD for torrent samples and sea sediment samples, respectively. Transparent fibres predominated, followed by fragments. The most common colour was white-cream. In total, 1.8% of items were analysed with Fourier transform infrared spectroscopy (FT-IR) 2D imaging, using a focal plane array (FPA) detector to identify their composition. Nylon was the most common plastic polymer type; however, approximately 50% of the analysed samples consisted of cotton transparent fibres. The influences of human activities (cities, port and discharges) on microlitter composition were highlighted and torrents were confirmed as a vector of microlitter from land to sea.

Plastic debris occurring in freshwater environments, which can either come from the surrounding terrestrial areas or transported from upstream, has been identified as one of the main sources and routes of plastic pollution in marine systems. The ocean is the final destination of land- based microplastic sources, but compared to marine environments, the occurrence and effects of microplastics in freshwater ecosystems remain largely unknown. A thorough examination of scientific literature on abundance, distribution patterns, and characteristics of microplastics in freshwater environments in Mediterranean tributary rivers has shown a substantial lack of information and the need to apply adequate and uniform measurement methods.

Sixteen priority polycyclic aromatic hydrocarbons (PAHs) regulated by the United States Environmental Protection Agency (USEPA) were analyzed in surface waters and riverine sediments of Brahmaputra and Hooghly Rivers, along urban-suburban-rural transects. ∑16 PAHs concentrations were higher in Hooghly riverine sediment (HRS) (Avg, 445 ng g−1) than Brahmaputra riverine sediment (BRS) (Avg, 169 ng g−1) dominated by 4-ring PAHs. In contrast, PAHs concentrations in surface water of Brahmaputra River (BRW) (Avg, 4.04 μg L−1) were comparable with Hooghly River (HRW) (Avg, 4.8 μg L−1), with dominance by 3-ring PAHs. Toxic PAHs (BaA, Chr, BbF, BkF, BaP, InP and DBA) were dominant in sub-urban transect of HRS (Avg, 387 ng g−1) and BRS (Avg, 14 ng g−1). Diagnostic ratios, principal component analysis (PCA) and ring wise composition suggested combustion as the main PAHs source in these riverine belts. In BRS, higher PAHs in suburban and rural transects were attributed to incomplete combustion of fossil fuel and biomass burning. In HRS, >85% of high molecular weight PAHs were found in the industrial areas of the suburban transect possibly associated with the discharge of industrial effluents. Harbor and port activities were other major contributors of HMW-PAHs in Hooghly riverine system. Carcinogenic potency estimated in terms of toxic equivalent (TEQ) was several folds higher in HRS (Avg, 106 ng TEQ g−1) compared with BRS (Avg, 2.5 ng TEQ g−1). Mostly low molecular weight PAHs are likely posing a risk to fishes in both the rivers. Risk on edible fish species may be a matter of concern considering the regular consumption of fishes in this region.

In the original publication, Figure 5 and the related text incorrectly referred to a polyurethane fibre; however, it was a possible PET-polyurethane blend.

Growth of informal electronic waste (e-waste) recycling sector is an emerging problem for India. The presence of halogenated compounds in e-wastes may result in the formation of persistent organic pollutants like polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) during recycling processes. We therefore investigated PCBs and PCDD/Fs in surface soils explicitly from the informal e-waste recycling sites and nearby open dumpsites of major metropolitan cities from four corners of India, viz., New Delhi (North), Kolkata (East), Mumbai (West) and Chennai (South). In the informal e-waste recycling sites, the range of Σ26PCBs (0.4–488 ng/g) and ƩPCDD/Fs (1.0–10.6 ng/g) were higher than Ʃ26PCBs (0.3–21 ng/g) and ƩPCDD/Fs (0.15–7.3 ng/g) from open dumpsites. In the e-waste sites, ƩPCDDs were found with increasing trend from ƩTetraCDD to OctaCDD, whereas ƩPCDFs showed a reverse trend. The dominance of PCDF congeners and maximum toxicity equivalents (TEQ) for both PCDDs (17 pg TEQ/g) and PCDFs (82 pg TEQ/g) at Mandoli in New Delhi has been related to intensive precious metal recovery process using acid bath. Among dumpsites, highest TEQ for PCDD/Fs was observed at Kodangaiyur dumpsite of Chennai (CNDS-02, 45 pg TEQ/g). Positive Matrix Factorization (PMF) model identified distinct congener pattern based on the functional activities, such as e-waste dismantling, shredding, precious metal recovery and open burning in dumpsites. E-waste metal recovery factor was loaded with 86–91% of PCB-77, -105, -114, -118 and 30% of PCB-126, possibly associated with the burning of wires during the copper extraction process. Almost 70% of the Ʃ26PCB concentrations was comprised of the dioxin-like PCB congeners with a maximum concentration of 437 ng/g at New Moore market in Chennai, followed by Wire Lane (102 ng/g), in Mumbai. We speculate that PCB-126 might have resulted from combustion of plastic materials in e-waste stream and dumped waste.

Microplastics pollution is progressively threatening natural parks across the world. In the framework of monitoring this concerning trend, the present study focuses on the occurrence and identification of mesoplastics (MEPs) and microplastics (MPs) in sand samples collected before and after the summer season from the beach of the Nature Park of Migliarino San Rossore Massaciuccoli (Pisa, Italy). Meso- and microplastics were identified using Fourier transform infrared spectroscopy 2D Imaging, and detected in all samples with average concentrations of 207 ± 30 MPs/kg d.w., and 100 ± 44 MEPs/kg d.w., respectively. Seasonal changes of flow of the Arno River, industrial activities, and urban footprint were considered as the major sources of plastic pollution. Our results showed the occurrence of both natural and synthetic polymers including cellulose, polyethylene, polypropylene, polyamides, polyethylene terephthalate, and acrylonitrile.

The current study determined, for the first time, the levels of titanium (Ti), strontium (Sr), barium (Ba), vanadium (V), gallium (Ga), tin (Sn), thallium (Tl), and antinomy (Sb), in deposited dust, and human hair collected from general population of different geographical areas of Pakistan. All the samples were prepared by microwave digestion and measured by ICP-MS. The results showed that on deposited dust samples, the detected elements followed the descending trend as: Ti > Sr > Ba > V > Ga > Sn > Tl > Sb similar to the upper continental crust. The deposited dust samples from low elevation areas exhibited highest levels of all studied elements (except antimony which was higher in soil samples from mountainous areas), followed by rive plains, mountainous areas, and highland valleys. In contrast, on human hair samples, the elements followed the descending trend as: Sr > Ba > Ti > Ga > V > Sn > Sb > Tl respectively. Ba, Ga, and V concentrations were higher in soil samples from lower elevation Indus plain, and Sr, Tl, Sb, and Ti were higher in samples from mountainous areas. The bioaccumulation trend of all studied elements was in descending order as follows: Sb, Ga, Sn, Ba, Sr, Ti, V, Tl, respectively. Principal component analysis (PCA) and correlation matrix evidenced both geological influences and anthropogenic activities as potential sources of these studied elements. On the other hand, the risk estimation (HI > 1) concluded that population were at higher health risk (non-carcinogenic) for Ga and Ti. All other studied rare elements were within safe limit for humans from all zones.

A sample preparation method was evaluated for the determination of polybrominated diphenyl ethers (PBDEs) in mussel samples, by using colorimetric and electrochemical immunoassay-based screening methods. Herein, a rapid procedure based on QuEChERS-like extraction approach followed by solid phase purification was optimized for PBDE extraction from mussel samples. The detection limits for colorimetric and electrochemical immunoassays, calculated as BDE-47 equivalent concentration, were 0.6 ng g−1 and 1.1 ng g−1, respectively. Real mussel samples, including a Certified Reference Material (CRM), were analyzed. The samples were measured by colorimetric and electrochemical immunoassays as well as by GC-MS. In comparison to GC-MS results, 106% and 102% relative accuracy were obtained for the colorimetric and electrochemical immunoassays, respectively. The proposed method could be useful for massive environmental campaigns, being able to rapidly detect possible polluted seafood samples.

This is the first survey to investigate the occurrence and extent of microplastic (MPs) contamination in sub surface waters collected near-shore and off-shore the coastal area of the Ross Sea (Antarctica). Moreover, a non-invasive method to analyze MPs, consisting in filtration after water sampling and analysis of the dried filter through Fourier Transform Infrared Spectroscopy (FTIR) 2D Imaging, using an FPA detector, was proposed. The non-invasiveness of analytical set-up reduces potential bias and allows subsequent analysis of the filter sample for determination of other classes of contaminants. MPs ranged from 0.0032 to 1.18 particle per m3 of seawater, with a mean value of 0.17 ± 0.34 particle m−3, showing concentrations lower than those found in the oceans worldwide. MPs included fragments (mean 71.9 ± 21.6%), fibers (mean 12.7 ± 14.3%), and others (mean 15.4 ± 12.8%). The presence of different types of MPs was confirmed by FTIR spectroscopy, with predominant abundance of polyethylene and polypropylene. The potential environmental impact arising from scientific activities, such as marine activities for scientific purposes, and from the sewage treatment plant, was also evidenced.