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Abstract There is no consensus on the optimal endpoint(s) in cancer cachexia trials. Endpoint variation is an obstacle when comparing interventions and their clinical value. The aim of this systematic review was to summarize and evaluate endpoints used to assess appetite and dietary intake in cancer cachexia clinical trials. A search for studies published from 1 January 1990 until 2 June 2021 was conducted using MEDLINE, Embase and Cochrane Central Register of Controlled Trials. Eligible studies examined cancer cachexia treatment versus a comparator in adults with assessments of appetite and/or dietary intake as study endpoints, a sample size ≥40 and an intervention lasting ≥14 days. Reporting was in line with PRISMA guidance, and a protocol was published in PROSPERO (2022 CRD42022276710). This review is part of a series of systematic reviews examining cachexia endpoints. Of the 5975 articles identified, 116 were eligible for the wider review series and 80 specifically examined endpoints of appetite (65 studies) and/or dietary intake (21 studies). Six trials assessed both appetite and dietary intake. Appetite was the primary outcome in 15 trials and dietary intake in 7 trials. Median sample size was 101 patients (range 40–628). Forty‐nine studies included multiple primary tumour sites, while 31 studies involved single primary tumour sites (15 gastrointestinal, 7 lung, 7 head and neck and 2 female reproductive organs). The most frequently reported appetite endpoints were visual analogue scale (VAS) and numerical rating scale (NRS) (40%). The appetite item from the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ) C30/C15 PAL (38%) and the appetite question from North Central Cancer Treatment Group anorexia questionnaire (17%) were also frequently applied. Of the studies that assessed dietary intake, 13 (62%) used food records (prospective registrations) and 10 (48%) used retrospective methods (24‐h recall or dietary history). For VAS/NRS, a mean change of 1.3 corresponded to Hedge's g of 0.5 and can be considered a moderate change. For food records, a mean change of 231 kcal/day or 11 g of protein/day corresponded to a moderate change. Choice of endpoint in cachexia trials will depend on factors pertinent to the trial to be conducted. Nevertheless, from trials assessed and available literature, NRS or EORTC QLQ C30/C15 PAL seems suitable for appetite assessments. Appetite and dietary intake endpoints are rarely used as primary outcomes in cancer cachexia. Dietary intake assessments were used mainly to monitor compliance and are not validated in cachexia populations. Given the importance to cachexia studies, dietary intake endpoints must be validated before they are used as endpoints in clinical trials.

Background: Undergraduate dental curricula and courses containing ergonomic principles are introduced to students from the very beginning of dental education. Still, dentists present a high prevalence of musculoskeletal disorders, which are a direct burden on quality of life, leading to early retirement from the profession. This study aimed to assess the state of students’ knowledge and awareness regarding the role of ergonomics in dentistry and its practical implementation. Methods: a cross-sectional study using a predesigned self-administered questionnaire was conducted among fourth- and fifth-year dental students of the Medical University of Silesia in Katowice, Poland (response rate of 69.2%). Results: A total of 94.6% of students declared a good, fair, or moderate level of knowledge of the subject, with a significant difference in favor of the fifth-year students (p = 0.008). Moreover, 76.1% of respondents showed a good or very good attitude toward ergonomics as a university curriculum subject. A total of 72.3% of respondents declared that the practical application of ergonomic rules in clinical dentistry is very important (five in a five-point scale). Women found dental ergonomics more important than men (p < 0.001). However, 79.3% of participants admitted not following basic ergonomic principles during clinical procedures. Conclusion: The research revealed a discrepancy between theoretical knowledge and awareness and the practical application of ergonomics in a clinical environment. Only by inculcating correct occupational standards and abiding by them from the commencement of dental education can consistency between theory and practice be achieved. This will hopefully ensure the health and well-being of dental team members throughout their professional lives.

Abstract In cancer cachexia trials, measures of physical function are commonly used as endpoints. For drug trials to obtain regulatory approval, efficacy in physical function endpoints may be needed alongside other measures. However, it is not clear which physical function endpoints should be used. The aim of this systematic review was to assess the frequency and diversity of physical function endpoints in cancer cachexia trials. Following a comprehensive electronic literature search of MEDLINE, Embase and Cochrane (1990–2021), records were retrieved. Eligible trials met the following criteria: adults (≥18 years), controlled design, more than 40 participants, use of a cachexia intervention for more than 14 days and use of a physical function endpoint. Physical function measures were classified as an objective measure (hand grip strength [HGS], stair climb power [SCP], timed up and go [TUG] test, 6‐min walking test [6MWT] and short physical performance battery [SPPB]), clinician assessment of function (Karnofsky Performance Status [KPS] or Eastern Cooperative Oncology Group‐Performance Status [ECOG‐PS]) or patient‐reported outcomes (physical function subscale of the European Organisation for the Research and Treatment of Cancer Quality of Life Questionnaires [EORTC QLQ‐C30 or C15]). Data extraction was performed using Covidence and followed PRISMA guidance (PROSPERO registration: CRD42022276710). A total of 5975 potential studies were examined and 71 were eligible. Pharmacological interventions were assessed in 38 trials (54%). Of these, 11 (29%, n = 1184) examined megestrol and 5 (13%, n = 1928) examined anamorelin; nutritional interventions were assessed in 21 trials (30%); and exercise‐based interventions were assessed in 6 trials (8%). The remaining six trials (8%) assessed multimodal interventions. Among the objective measures of physical function (assessed as primary or secondary endpoints), HGS was most commonly examined (33 trials, n = 5081) and demonstrated a statistically significant finding in 12 (36%) trials (n = 2091). The 6MWT was assessed in 12 trials (n = 1074) and was statistically significant in 4 (33%) trials (n = 403), whereas SCP, TUG and SPPB were each assessed in 3 trials. KPS was more commonly assessed than the newer ECOG‐PS (16 vs. 9 trials), and patient‐reported EORTC QLQ‐C30 physical function was reported in 25 trials. HGS is the most commonly used physical function endpoint in cancer cachexia clinical trials. However, heterogeneity in study design, populations, intervention and endpoint selection make it difficult to comment on the optimal endpoint and how to measure this. We offer several recommendations/considerations to improve the design of future clinical trials in cancer cachexia.

Understanding the spatial–temporal variability of soil enzymatic activity and its relationship with nitrogen (N) resources in the soil and crop yield is crucial in rational management practices of mineral fertilization. The scarcity of comprehensive studies on geostatic analyses of agricultural soils and plant yields, which would take into account both temporal and spatial variability, was the reason for undertaking this research. The aim of this study was to determine the spatial and temporal variability of the activity of soil enzymes, such as acid (PAC) and alkaline (PAL) phosphatases, urease (URE) and protease (PROT), the content of N-NH4 (ammonium ions), N-NO3 (nitrate ions), phosphorus (P), pH, moisture, as well as crop yield on a conventionally managed farmland of 40 ha. During the two-year experiment, soil samples were collected from 37 measurement points. Wheat was the first tested crop, followed by oilseed rape. It was shown that all the tested soil parameters showed temporal and spatial variability, and a significant number of them were significantly higher in July. The creation of raster maps showing the distribution of the tested parameters allowed for the observation of the considerable activity of PAC, PAL, URE, and PROT, as well as a high application of N-NO3 in the southern part of the field during the growth of the plants. The statistical analysis revealed a negative interaction between the N-NH4 and N-NO3 and the urease in the soil under the cultivation of plants. The pH and the percentage of moisture in the soil also had higher values in the south of the field. This pointed to the existence of separate production zones in the south-central part of the field, characterized by a higher yield of wheat and rape. On the basis of the conducted research, it was unequivocally stated that the values of enzymatic and chemical parameters of the soil were reflected in the size of the yield obtained, which allows conclusions to be drawn with respect to the rational management of N in the production process, laying the foundations for precision agriculture.

The size of the microbial biomass and the activity of soil enzymes are among the most sensitive indicators of agricultural land quality. The aim of this study was to determine the spatial and temporal variability of microbial biomass, the activity of dehydrogenase (DHA) enzyme and the concentration of micro- (Na, Mg and Ca) and macroelements (Cu, Zn, Mn and Fe) in the soil, collected from 37 measurement sites (depth 0–30 cm) in a 40-hectare field during two growing seasons (wheat and oilseed rape). The percentage of nitrogen (%N) in the wheat grain and rapeseeds was also determined. Mapping the spatial distribution of the microbial biomass, the level of DHA activity and the concentration of the selected elements was used to assess the soil productivity. All tested soil parameters exhibited temporal and spatial variability. The creation of raster maps showing the distribution of the tested parameters allowed the observation of a higher nitrogen content in wheat grains in the south-western part of the field, with high values of DHA activity, bacterial biomass and soil pH. In the case of oilseed rape, plants grown in the northern part of the field were characterized by a higher nitrogen content in the grain, where the soil was characterized by a higher content of fungal biomass. On the basis of the obtained research results, a positive, statistically significant correlation was also shown between the biomass of bacteria and the level of DHA in the soil under the cultivation of both wheat and rape. The cultivation of both crops had a significant impact on the size of the microbial biomass pool and on the DHA activity level but did not affect the concentration of the nutrients in the soil. High concentrations of the analyzed elements at the measuring points correlated with a greater %N content in the grain/seeds of the crops harvested at those locations in the field. The results conclude that the mapping of the physicochemical parameters, microbial biomass and activity on the field permits the development of an effective strategy for maintaining sustainable soil productivity through the appropriate management of agricultural practices and the better approximation of mineral fertilization.

Nitrogen (N) is widely distributed in the lithosphere, hydrosphere, atmosphere and biosphere. It is a basic component of every plant cell as well as microorganisms, as a component of proteins, nucleic acids and chlorophyll. It enters soil with organic and mineral fertilizers, plant and animal residues and biological nitrogen fixation. There are various forms of nitrogen in soil, and this element is usually transformed by microorganisms. The transformation of nitrogen compounds (ammonification, nitrification and immobilization) is significantly influenced by climatic conditions and the physicochemical properties of soil. Microbial mineralization of nitrogen organic matter results in the enrichment of soil with this element, which is necessary to generate a yield. The amount of nitrogen entering soil through the mineralization of crop residues ranges from 15 to 45 kg N/ha in cereal residues and from 80 to 144 kg N/ha in winter rape residues. Biological nitrogen fixation can increase the nitrogen content in soil by 30–50 kg/ha/year. In recent decades, the mismanagement of mineral fertilizers has drastically changed the natural balance of the nitrogen cycle. Every year huge amounts of nitrogen compounds enter the aquatic ecosystems and cause their eutrophication. That is why it is important to have adequate knowledge of sustainable fertilization so as to practice integrated crop management.

The article aims to present results of research on anaerobic digestion (AD) of waste wafers (WF-control) and co-substrate system–waste wafers and cheese (WFC-control), combined with digested sewage sludge, as inoculum. The purpose of this paper is to confirm the outcome of adding silica/lignin (S/L; 4:1) material, as a microbial carrier, on the process performance and genetic diversity of microbial communities. The experiment was conducted in a laboratory under mesophilic conditions, in a periodical operation mode of bioreactors. Selected physicochemical parameters of the tested carrier, along with the microstructure and thermal stability, were determined. Substrates, batches and fermenting slurries were subjected to standard parameter analysis. As part of the conducted analysis, samples of fermented food were also tested for total bacterial count, dehydrogenase activity. Additionally, DNA extraction and next-generation sequencing (NGS) were carried out. As a result of the conducted study, an increase in the volume of produced biogas was recorded for samples fermented with S/L carrier: in the case of WF + S/L by 18.18% to a cumulative biogas yield of 833.35 m3 Mg−1 VS, and in the case of WFC + S/L by 17.49% to a yield of 950.64 m3 Mg−1 VS. The largest total bacterial count, during the process of dehydrogenase activity, was maintained in the WFC + S/L system. The largest bacterial biodiversity was recorded in samples fermented with the addition of cheese, both in the case of the control variant and in the variant when the carrier was used. In contrast, three phyla of bacteria Firmicutes, Proteobacteria and Actinobacteria predominated in all experimental facilities.

The taxonomic and functional diversity of bacteria in seven different experimental variants applied to soil under a maize plantation was determined by means of next-generation sequencing and biochemical methods. The aim of the study was to discover differences in the structure of bacteria and the level of soil enzymatic activity (BIF—biochemical index of fertility) after the application of a biofertiliser made of lignocellulosic substrate and biochar containing various microorganisms (algae, mycorrhizal fungi of the Glomus genus or the consortium of Bacillus sp. bacteria). The chemical composition and yield of crops was a measurable indicator of the effectiveness of the fertilisers. The biofertilisers influenced both the structure and the percentage share of individual bacterial operational taxonomic units (OTU). The cultivation of maize also modified qualitative and quantitative changes in the soil bacterial microbiome. A canonical variate analysis (CVA) showed that the soil pH exhibited a minimal positive correlation with the soil enzymatic activity and selected plant parameters, with the exception of the biofertiliser variant with arbuscular mycorrhiza (AM). Moreover, the AM biofertiliser significantly increased the BIF value, the yield of maize seeds and the starch content in the plants. The comprehensive nature of the research allowed for a deepening and systematization of the existing knowledge on the influence of biochar with the addition of selected microorganisms on the biochemical parameters of the soil and the bacterial biodiversity of the soil environment. Additionally, the inclusion of the chemical, sanitary composition and yield of maize in the research brought a measurable view of the changes taking place in the soil and plant environment under the influence of the discussed factor. Apart from the agronomic aspect (integrated crop cultivation—Directive 2009/128/EC) of our study, it was also closely related to environmental protection, as it proved that biochar-based biofertilisers could be an alternative to mineral fertilisation.

The aim of this article is to present the issues related to the significance of microorganisms in the mineralization of crop residues and the influence of environmental factors on the rate of this process. Crop residues play a very important role in agriculture because they can be used both as an environment-friendly waste management strategy and as a means of improving soil organic matter. The inclusion of crop residues in the soil requires appropriate management strategies that support crop production and protect the quality of surface water and groundwater. Crops need nutrients for high yields; however, they can only absorb ionic forms of elements. At this point, the microorganisms that convert organically bound nitrogen, phosphorus, and sulfur into soluble NH4+, NO3−, H2PO4−, HPO42−, and SO42− ions are helpful. Mineralization is the transformation of organic compounds into inorganic ones, which is a biological process that depends on temperature, rainfall, soil properties, the chemical composition of crop residues, the structure and composition of microbial communities, and the C:N ratio in soil after the application of plant matter. An adjustment of the values of these factors enables us to determine the rate and direction of the mineralization of crop residues in soil.

The combination of polymeric materials used in 3D printing and carbon fibers gives designers new possibilities in the design of light load-bearing structures. Such a reinforced material can be found in thin-walled structures. A good example is the wheel of a Mars rover, the structure of which is expected to be low in weight, with adequate strength and durability as well as deformations within the assumed range - ensuring vibration damping in the rigid suspension of a special vehicle. The paper presents the process of designing such a wheel within the limits of the Europen Rover Challenge competition regulations and the technological possibilities of a low-budget project. The conducted experiment confirmed the correctness of the assumptions and indicated potential directions for further work.