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Your search keyword '"Alejandro Krimer"' showing total 3 results
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3 results on '"Alejandro Krimer"'

1. Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates

Author

Andris Kazaks, I-Na Lu, Sophie Farinelle, Alex Ramirez, Vincenzo Crescente, Benjamin Blaha, Olotu Ogonah, Tarit Mukhopadhyay, Mapi Perez de Obanos, Alejandro Krimer, Inara Akopjana, Janis Bogans, Velta Ose, Anna Kirsteina, Tatjana Kazaka, Nicola J. Stonehouse, David J. Rowlands, Claude P. Muller, Kaspars Tars, and William M. Rosenberg

Subjects
Virus-like particles, Tandem-core, Influenza vaccine, Long alpha helix, Yeast, Biotechnology, TP248.13-248.65
Abstract

Abstract Background The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus strains. The long alpha helix (LAH) is an attractive vaccine component since it is one of the most conserved influenza hemagglutinin (HA) stalk regions. For an improved immune response, the LAH domain from H3N2 strain has been incorporated into virus-like particles (VLPs) derived from hepatitis B virus core protein (HBc) using recently developed tandem core technology. Results Fermentation conditions for recombinant HBc-LAH were established in yeast Pichia pastoris and a rapid and efficient purification method for chimeric VLPs was developed to match the requirements for industrial scale-up. Purified VLPs induced strong antibody responses against both group 1 and group 2 HA proteins in mice. Conclusion Our results indicate that the tandem core technology is a useful tool for incorporation of highly hydrophobic LAH domain into HBc VLPs. Chimeric VLPs can be successfully produced in bioreactor using yeast expression system. Immunologic data indicate that HBc VLPs carrying the LAH antigen represent a promising universal influenza vaccine component.

Published
2017
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2. Foot and mouth disease (FMD) virus: quantification of whole virus particles during the vaccine manufacturing process by size exclusion chromatography

Author

Eliana Smitsaart, Marcelo A. Spitteler, Erika Schabes, M. Susana Levy, Emmanuel G. Régulier, Alejandro Krimer, Mariela Guinzburg, and Ignacio Fernández

Subjects
General Veterinary, General Immunology and Microbiology, biology, Foot-and-mouth disease, Viral Vaccine, Size-exclusion chromatography, Public Health, Environmental and Occupational Health, Viral Vaccines, Viral Load, biology.organism_classification, medicine.disease, Virology, Virus, Infectious Diseases, Vaccine manufacturing, Vaccines, Inactivated, Foot-and-Mouth Disease Virus, medicine, Chromatography, Gel, Molecular Medicine, Technology, Pharmaceutical, Viral disease, Foot-and-mouth disease virus, Viral load
Abstract

Foot and mouth disease (FMD) is a highly infectious viral disease that affects cattle, sheep, goats and swine causing severe economic losses worldwide. The efficacy of inactivated vaccines is critically dependent on the integrity of foot and mouth disease virus (FMDV) particles. The recommended method to quantify the active ingredient of vaccines is the 140S quantitative sucrose density gradient analysis. This method has been an immensely valuable tool over the past three decades but it is highly operator dependent and difficult to automate. We developed a method to quantify FMDV particles during the vaccine manufacturing process that is based on separation of components by size-exclusion chromatography and measurement of virus by absorption at 254nm. The method is linear in the 5-70μg/mL range, it is applicable to different FMDV strains, and has a good correlation with the 140S test. The proposed method uses standard chromatographic media and it is amenable to automation. The method has potential as a process analytical technology and for control of final product by manufacturers, international vaccine banks and regulatory agencies.

Published
2011

3. First molecular characterization of canine hepatozoonosis in Argentina: evaluation of asymptomatic Hepatozoon canis infection in dogs from Buenos Aires

Author

Diego Fernando Eiras, María L. Matos, Julia Basabe, Alejandro Krimer, Gad Baneth, Diana B. Banach, and Carla F. Scodellaro

Subjects
Male, Veterinary medicine, Argentina, Parasitemia, Asymptomatic, 18S ribosomal RNA, Dogs, Ticks, parasitic diseases, medicine, Animals, Dog Diseases, Genotyping, Hepatozoon canis, General Veterinary, biology, Coccidiosis, Ciencias Veterinarias, General Medicine, biology.organism_classification, medicine.disease, Hepatozoon sp, Virology, Coccidia, Hepatozoon, Canis, PCR, Parasitology, Female, medicine.symptom
Abstract

Canine hepatozoonosis is an expanding tick-borne disease in Argentina. Hepatozoonosis was studied during 1 year in six dogs from the same household in Buenos Aires. Blood parasitemia with Hepatozoon gamonts was found in five dogs and all six were positive by PCR for Hepatozoon sp. Although the levels of parasitemia fluctuated during the year, no clinical signs of disease were detected during the follow up period. Amplification and sequencing of a 650 bases fragment of the 18S rRNA gene from all six dogs yielded fragments that were 99% identical to H. canis. The results of the partial 18S rRNA genotyping with the sub-clinical course of infection and lack of severe hematological abnormalities are compatible with clinical and molecular descriptions of Hepatozoon canis infection from other areas of the world. This is the first molecular characterization of Hepatozoon from Argentina., Facultad de Ciencias Veterinarias

Published
2007

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