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1. Optimization of process conditions for the production of a prolylendopeptidase by Aspergillus niger ATCC 11414 in solid state fermentation

Author

Luz Neira, Alejandro Huaiquil, Carolina Shene, Mónica Rubilar, Yussef Esparza, Luis A. Salazar, and Allison Leyton

Subjects
Chromatography, Protease, biology, Chemistry, medicine.medical_treatment, Aspergillus niger, Substrate (chemistry), biology.organism_classification, Applied Microbiology and Biotechnology, Spore, Prolyl endopeptidase, Solid-state fermentation, Protein purification, medicine, Ammonium sulfate precipitation, Food Science, Biotechnology, medicine.drug
Abstract

The effect of 8 factors [(with/without) daily mixing and moisture control, incubation time (t), temperature, ratio between dry substrate mass and bed’s cross section area (MA), inoculum size (spores/g), wheat germ content (WG), initial pH, and moisture content (M)] in the production of a prolyl endopeptidase (PEP) by Aspergillus niger ATCC 11414 in solid state fermentation (SSF) was tested. Contribution of all the factors was significant (p

Published
2011

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