Poor agricultural practices have caused eroded soils, therefore alternatives are sought for the solubilization and fixation of nutrients by mycorrhizal fungi, that grow on the plants roots forming a mutualist symbiosis. Therefore, in this work, Basidiomycetes fungi were isolated and those that are able to solubilizing phosphorus and nitrogen were selected for their inoculation in lettuce (Lactuca sativa), this is high consumption and fast growth vegetable. Five substrates (fertile soil, eroded soil, humus and horse manure) were studied with a not inoculated control, where the response variable was hypocotyl growth and the L. sativa radicle, measured every 24 hours for 30 days. It was observed that the HM3 fungi stimulated the highest growth of the plant in eroded soil, generating an alternative to agriculture and contributing to bioremediation and exploitation of damaged soils